Lsi10G000950 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G000950
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cysteine proteinase 1
Location	chr10: 1518714 .. 1520941 (-)
RNA-Seq Expression	Lsi10G000950
Synteny	Lsi10G000950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGGTCAGAGGAGAGGGAGAGATGATTGAGAAAGAAAAGAAAAATATTTAAAATAATATAAAAAATACTTAATTAATGTAGTAGAAAAGTTTTGGCTAACTTGGCCAAATTAATACTAAGGGACCAAATATACCTAGTCCTAAAAATTCGACGACTAAAAAGATATTTTTTTTCCTAGAATTAAACTTTATGATACCAACCTAACTGAGATATTTAAACACTATGAGTATCCTAAAACTAAGGGGTATTTGGGCGTTGGGTTGGTAATTATAATCAATAGTTTTTATTGTCTATGGGTTATGATAGTTTGTAGGTTATAATAGTCTATGTTTGGGATGCATACTATTTGAATTGTTTGAGTAGGAAATAACAAACATGGTAACAAAGAGAGAAAAAGAGGATGAGTAGGAAATATTAAACACTATAACAAAAAGCAATAATCGCAGGTTATAATAGTTGAAAATACCAACTATTATAATTGGAACCTTAAACATAAAGTGAACTATAATAGTTCACTCCACCTACTTGAAGTTGAAACCCAAACACCCCCTAAATATGGTAGGTTCAACCATTTGTGCTAACCTTTGTTCAATGTCATTTTTAATTGTTAGATTTCTTATTTCTCATTTTTTGTTTAATTATTTACGGGAAAAATGTGATTATTCATTTCTCACCTTTTTTTATGAACTTTAATCATTTTACTTACAAGAACACTTGCATTATTATGTAGTACACCCAAATCATTTTCTACCACAGAAGTGAAGCACAATTGATCTATTTTCTTTTCGTGGGTATCTATCTTAAGCAGAAATGTAGTGTTTCCACCCTTTTTTGCCGTGCTGGAAAGATGAAACAACAGACTAGCCCCTACTGAAACTTCAAGGTGTGAAATACATGAATCCAAGAATTTCTTAAGTATCAAAACTTCATCCTCTTGCAGTTGGGAATGGATCAATATATAACTAGAATTGATGTGGAGAATGAAAACATAATTAGCTCGATCCGCAATCGGAAAAAGGCCATGGAAGCATATAGAATGATTTGGAATGTGGGTTTGATGTCTCTGATTCTCTGGGTTTTCTGGACACCCTCAATGGTATCCATGGCAATGGACTACCCTCCAGGATCTGATTCCAGTGACTTACAAGACAGGTACCAGAATTGGATGAGTAAATACGGTCGAGAATACAAGAGCAGAGAAGAGCGGGAGCGGAGATTCACAATTTATCAGTTGAATGTTCAGTACATTGACAACTTCAATTCTCTGAATCATTCATATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGATGAGTTCAAGACAACTTACTTGGGGTATCGAACTGATTGGCTTCCTGATACATGCTTCAGATATGGAAATATGGTAAATTTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTTACTCCAATAAAGAATCAAGGCCAATGTGGTATGAACTCATAATACTCATTCATCTAACTAATCAGAAATCAGAATGAAAATGTTCTCTTTTCGTCGGATTTTGAGTTCTTTTTCCTAACAACTACCCTACCCAACTCTATCCATTGTAAGTCATCCCTTCGTATTTGAACAAACTCACCAGTTTTGAATTTTTGACACAGGGAGCTGCTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCAACAAAATAAAAACAGGCAAATTGATGTCTCTATCAGAACAAGAGCTTGTGGACTGCGATGTGGTCTCGGGGAACCAGGGATGCAATGGTGGTTACATGTACAAAGCATTTGAGTTCGTCAAGAAAAGTGGAATCACTACCGAAACAGAATATCCATACAGGGGAACTGAATCTGTATGCAACAAACAAAAAGTGAGATACCACACTGTGACAATAACTGGATATGAAAAAGTACCTGTCAATGATGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGGGGGATATGATTTTCAGTTCTATTCTGGTGGAGTCTACTCAGGCAATTGTGGAAAGCAACTCAATCATGGAGTGTCAATAGTTGGGTATGGGGAAGCTAGTAGTAAACCTTATTGGCTTGTTAAGAATTCATGGGGCACTGACTGGGGTGAATCTGGTTATATAAGAATGAAACGTGATTCAACCGACAAGCGAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCATCAAAGACTGA

mRNA sequence

ATGACGTTGGGAATGGATCAATATATAACTAGAATTGATGTGGAGAATGAAAACATAATTAGCTCGATCCGCAATCGGAAAAAGGCCATGGAAGCATATAGAATGATTTGGAATGTGGGTTTGATGTCTCTGATTCTCTGGGTTTTCTGGACACCCTCAATGGTATCCATGGCAATGGACTACCCTCCAGGATCTGATTCCAGTGACTTACAAGACAGGTACCAGAATTGGATGAGTAAATACGGTCGAGAATACAAGAGCAGAGAAGAGCGGGAGCGGAGATTCACAATTTATCAGTTGAATGTTCAGTACATTGACAACTTCAATTCTCTGAATCATTCATATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGATGAGTTCAAGACAACTTACTTGGGGTATCGAACTGATTGGCTTCCTGATACATGCTTCAGATATGGAAATATGGTAAATTTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTTACTCCAATAAAGAATCAAGGCCAATGTGGGAGCTGCTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCAACAAAATAAAAACAGGCAAATTGATGTCTCTATCAGAACAAGAGCTTGTGGACTGCGATGTGGTCTCGGGGAACCAGGGATGCAATGGTGGTTACATGTACAAAGCATTTGAGTTCGTCAAGAAAAGTGGAATCACTACCGAAACAGAATATCCATACAGGGGAACTGAATCTGTATGCAACAAACAAAAAGTGAGATACCACACTGTGACAATAACTGGATATGAAAAAGTACCTGTCAATGATGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGGGGGATATGATTTTCAGTTCTATTCTGGTGGAGTCTACTCAGGCAATTGTGGAAAGCAACTCAATCATGGAGTGTCAATAGTTGGGTATGGGGAAGCTAGTAGTAAACCTTATTGGCTTGTTAAGAATTCATGGGGCACTGACTGGGGTGAATCTGGTTATATAAGAATGAAACGTGATTCAACCGACAAGCGAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCATCAAAGACTGA

Coding sequence (CDS)

ATGACGTTGGGAATGGATCAATATATAACTAGAATTGATGTGGAGAATGAAAACATAATTAGCTCGATCCGCAATCGGAAAAAGGCCATGGAAGCATATAGAATGATTTGGAATGTGGGTTTGATGTCTCTGATTCTCTGGGTTTTCTGGACACCCTCAATGGTATCCATGGCAATGGACTACCCTCCAGGATCTGATTCCAGTGACTTACAAGACAGGTACCAGAATTGGATGAGTAAATACGGTCGAGAATACAAGAGCAGAGAAGAGCGGGAGCGGAGATTCACAATTTATCAGTTGAATGTTCAGTACATTGACAACTTCAATTCTCTGAATCATTCATATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGATGAGTTCAAGACAACTTACTTGGGGTATCGAACTGATTGGCTTCCTGATACATGCTTCAGATATGGAAATATGGTAAATTTGCCTACTAATGTTGACTGGAGAAAGGAAGGTGCAGTTACTCCAATAAAGAATCAAGGCCAATGTGGGAGCTGCTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCAACAAAATAAAAACAGGCAAATTGATGTCTCTATCAGAACAAGAGCTTGTGGACTGCGATGTGGTCTCGGGGAACCAGGGATGCAATGGTGGTTACATGTACAAAGCATTTGAGTTCGTCAAGAAAAGTGGAATCACTACCGAAACAGAATATCCATACAGGGGAACTGAATCTGTATGCAACAAACAAAAAGTGAGATACCACACTGTGACAATAACTGGATATGAAAAAGTACCTGTCAATGATGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGGGGGATATGATTTTCAGTTCTATTCTGGTGGAGTCTACTCAGGCAATTGTGGAAAGCAACTCAATCATGGAGTGTCAATAGTTGGGTATGGGGAAGCTAGTAGTAAACCTTATTGGCTTGTTAAGAATTCATGGGGCACTGACTGGGGTGAATCTGGTTATATAAGAATGAAACGTGATTCAACCGACAAGCGAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCATCAAAGACTGA

Protein sequence

MTLGMDQYITRIDVENENIISSIRNRKKAMEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD

Homology

[Close]

BLAST of Lsi10G000950 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.5e-94
Identity = 183/346 (52.89%), Postives = 234/346 (67.63%), Query Frame = 0


Query: 42  MSLILWVFWTPSM---VSMAMDYPPGSDSSD--LQDRYQNWMSKYGREYKSREERERRFT 101
           M  +LWV  + S+   V+ + D+      S+  L D Y+ W S +    +S  E+ +RF 
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHH-TVSRSLGEKHKRFN 62

Query: 102 IYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLG--------YRTDWLPDTCFR 161
           +++ NV ++ N N ++  Y L  N FAD+TN EF++TY G        +R        F 
Sbjct: 63  VFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFM 122

Query: 162 YGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELV 221
           Y  + ++P +VDWRK+GAVT +K+QGQCGSCWAFS + AVEGIN+IKT KL+SLSEQELV
Sbjct: 123 YEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 182

Query: 222 DCDVVSGNQGCNGGYMYKAFEFVK-KSGITTETEYPYRGTESVCNKQKVRYHTVTITGYE 281
           DCD    NQGCNGG M  AFEF+K K GITTE+ YPY   E  C++ KV    V+I G+E
Sbjct: 183 DCD-KEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHE 242

Query: 282 KVPVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYG-EAS 341
            VPVNDE +L  AVANQPVSVAIDAGG DFQFYS GV++G+C   LNHGV+IVGYG    
Sbjct: 243 NVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTVD 302

Query: 342 SKPYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
              YW+V+NSWG +WGE GYIRM+R+ + K G CGIAMMASYPIK+
Sbjct: 303 GTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKN 346

[Close]

BLAST of Lsi10G000950 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 342.8 bits (878), Expect = 4.8e-93
Identity = 181/355 (50.99%), Postives = 237/355 (66.76%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPGSDSSD--LQDRYQNWMSKYGREYKS 89
           M   +++W V   SL+L        V+ + D+     +S+  L D Y+ W S +    +S
Sbjct: 1   MATKKLLWVVLSFSLVLG-------VANSFDFHDKDLASEESLWDLYERWRSHH-TVSRS 60

Query: 90  REERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLG--------YRT 149
             E+ +RF +++ N+ ++ N N ++  Y L  N FAD+TN EF++TY G        +R 
Sbjct: 61  LGEKHKRFNVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRG 120

Query: 150 DWLPDTCFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKL 209
               +  F Y  +V++P +VDWRK+GAVT +K+QGQCGSCWAFS V AVEGIN+IKT KL
Sbjct: 121 TPHENGAFMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKL 180

Query: 210 MSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVK-KSGITTETEYPYRGTESVCNKQKVRY 269
           ++LSEQELVDCD    NQGCNGG M  AFEF+K K GITTE+ YPY+  E  C+  KV  
Sbjct: 181 VALSEQELVDCD-KEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVND 240

Query: 270 HTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVS 329
             V+I G+E VP NDE +L  AVANQPVSVAIDAGG DFQFYS GV++G+C   LNHGV+
Sbjct: 241 LAVSIDGHENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVA 300

Query: 330 IVGYG-EASSKPYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           IVGYG       YW+V+NSWG +WGE GYIRM+R+ + K G CGIAM+ SYPIK+
Sbjct: 301 IVGYGTTVDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIKN 346

[Close]

BLAST of Lsi10G000950 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-92
Identity = 177/313 (56.55%), Postives = 215/313 (68.69%), Query Frame = 0


Query: 70  LQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDE 129
           L   Y  W S +    +S  ERE+RF +++ NV ++ N N  N SY L  N FADLT +E
Sbjct: 34  LSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINE 93

Query: 130 FKTTYLG-----YRTDWLP-----DTCFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSC 189
           FK  Y G     +R    P        + + N+  LP++VDWRK+GAVT IKNQG+CGSC
Sbjct: 94  FKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSC 153

Query: 190 WAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVKKS-GITT 249
           WAFS VAAVEGINKIKT KL+SLSEQELVDCD    N+GCNGG M  AFEF+KK+ GITT
Sbjct: 154 WAFSTVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCNGGLMEIAFEFIKKNGGITT 213

Query: 250 ETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQ 309
           E  YPY G +  C+  K     VTI G+E VP NDE +L  AVANQPVSVAIDAG  DFQ
Sbjct: 214 EDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQ 273

Query: 310 FYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWLVKNSWGTDWGESGYIRMKRDSTDKRG 369
           FYS GV++G+CG +LNHGV+ VGYG    K YW+V+NSWG +WGE GYI+++R+  +  G
Sbjct: 274 FYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEG 333

Query: 370 TCGIAMMASYPIK 372
            CGIAM ASYPIK
Sbjct: 334 RCGIAMEASYPIK 344

[Close]

BLAST of Lsi10G000950 vs. ExPASy Swiss-Prot
Match: Q7XWK5 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. japonica OX=39947 GN=SAG39 PE=2 SV=2)

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-92
Identity = 171/315 (54.29%), Postives = 223/315 (70.79%), Query Frame = 0


Query: 65  SDSSDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFAD 124
           SD + +  R++ WM++YGR Y+   E+ RRF +++ NV +I++FN+ NH++ L  N FAD
Sbjct: 28  SDDAAMAARHERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFAD 87

Query: 125 LTNDEFKTTYLGYRTDWLPDTC-----FRYGNMVN---LPTNVDWRKEGAVTPIKNQGQC 184
           LTNDEF+  ++     ++P T      FRY N VN   LP  VDWR +GAVTPIK+QGQC
Sbjct: 88  LTNDEFR--WMKTNKGFIPSTTRVPTGFRYEN-VNIDALPATVDWRTKGAVTPIKDQGQC 147

Query: 185 GSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEF-VKKSG 244
           G CWAFSAVAA+EGI K+ TGKL+SLSEQELVDCDV   +QGC GG M  AF+F +K  G
Sbjct: 148 GCCWAFSAVAAMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGG 207

Query: 245 ITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGY 304
           +TTE+ YPY   +  C  + V     +I GYE VP N+E +L  AVANQPVSVA+D G  
Sbjct: 208 LTTESNYPYAAADDKC--KSVSNSVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDM 267

Query: 305 DFQFYSGGVYSGNCGKQLNHGVSIVGYGEAS-SKPYWLVKNSWGTDWGESGYIRMKRDST 364
            FQFY GGV +G+CG  L+HG+  +GYG+AS    YWL+KNSWGT WGE+G++RM++D +
Sbjct: 268 TFQFYKGGVMTGSCGTDLDHGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKDIS 327

Query: 365 DKRGTCGIAMMASYP 370
           DKRG CG+AM  SYP
Sbjct: 328 DKRGMCGLAMEPSYP 337

[Close]

BLAST of Lsi10G000950 vs. ExPASy Swiss-Prot
Match: A2XQE8 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_14861 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.4e-92
Identity = 172/315 (54.60%), Postives = 222/315 (70.48%), Query Frame = 0


Query: 65  SDSSDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFAD 124
           SD + +  R++ WM++YGR Y+   E+ RRF +++ NV +I++FN+ NH++ L  N FAD
Sbjct: 28  SDDAAMAARHERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFAD 87

Query: 125 LTNDEFKTTYLGYRTDWLPDTC-----FRYGNMVN---LPTNVDWRKEGAVTPIKNQGQC 184
           LTNDEF+ T       ++P T      FRY N VN   LP  VDWR +GAVTPIK+QGQC
Sbjct: 88  LTNDEFRWTKT--NKGFIPSTTRVPTGFRYEN-VNIDALPATVDWRTKGAVTPIKDQGQC 147

Query: 185 GSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEF-VKKSG 244
           G CWAFSAVAA+EGI K+ TGKL+SLSEQELVDCDV   +QGC GG M  AF+F +K  G
Sbjct: 148 GCCWAFSAVAAMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGG 207

Query: 245 ITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGY 304
           +TTE+ YPY   +  C  + V     +I GYE VP N+E +L  AVANQPVSVA+D G  
Sbjct: 208 LTTESNYPYAAADDKC--KSVSNSVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDM 267

Query: 305 DFQFYSGGVYSGNCGKQLNHGVSIVGYGEAS-SKPYWLVKNSWGTDWGESGYIRMKRDST 364
            FQFY GGV +G+CG  L+HG+  +GYG+AS    YWL+KNSWGT WGE+G++RM++D +
Sbjct: 268 TFQFYKGGVMTGSCGTDLDHGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKDIS 327

Query: 365 DKRGTCGIAMMASYP 370
           DKRG CG+AM  SYP
Sbjct: 328 DKRGMCGLAMEPSYP 337

[Close]

BLAST of Lsi10G000950 vs. ExPASy TrEMBL
Match: A0A384S0D9 (Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.4e-172
Identity = 291/319 (91.22%), Postives = 304/319 (95.30%), Query Frame = 0


Query: 54  MVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNH 113
           M SM MDY PGS S DLQDRYQ WMSKYGREYKSREE E+RF IYQLNVQYIDNFNSLNH
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 114 SYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRYGNMVNLPTNVDWRKEGAVTPIKNQ 173
           SYTLAEN+FADLTNDEFKTTYLG++TDWLPDT FRYGNMVNLPTNVDWRKE AVTP+K+Q
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 174 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVKK 233
           GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDV SGNQGCNGGYMYKAFEF+KK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 234 SGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAG 293
           +G+TTE EYPYRG ESVCNKQKVRY TVTI+GYEKVPVNDEKSLKAAVANQPVSVAIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 294 GYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWLVKNSWGTDWGESGYIRMKRDS 353
           GYDFQFYSGGV+SGNCGKQLNHGV+IVGYGEAS+K YWLVKNSWGTDWGESGYIRMKRDS
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDS 300

Query: 354 TDKRGTCGIAMMASYPIKD 373
           TDKRGTCGIAMMASYPIKD
Sbjct: 301 TDKRGTCGIAMMASYPIKD 319

[Close]

BLAST of Lsi10G000950 vs. ExPASy TrEMBL
Match: A0A5A7SQK0 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001250 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 289/346 (83.53%), Postives = 313/346 (90.46%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPG-SDSSDLQDRYQNWMSKYGREYKSR 89
           MEAY+MIW+V L+SLILWVFWTP+ VSMAMDYP G S+S +LQDRYQ WM KYGR+YKSR
Sbjct: 1   MEAYKMIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSR 60

Query: 90  EERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLP--DTC 149
           EE E+RFTIYQ NVQYIDNFNSLNHSYTLAENNF DLTN+EF  TYLGY T  LP  DT 
Sbjct: 61  EEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTF 120

Query: 150 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQE 209
           FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIK GKLMSLSEQE
Sbjct: 121 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQE 180

Query: 210 LVDCDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGY 269
           LVDCDV SGNQGCNGGYMYKAFEF+KK+G+TTE EYPY  T S C+KQK +Y +V+I+GY
Sbjct: 181 LVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGY 240

Query: 270 EKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEAS 329
           EKVPVNDEKSL+AAVA QPVSVAIDAGG DFQFYSGG++SGNCGKQLNHGV+IVGYGE S
Sbjct: 241 EKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDS 300

Query: 330 SKPYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           ++ YWLVKNSWGT WGESGYIRM RDSTDK+GTCGIAMMASYPIKD
Sbjct: 301 NQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

[Close]

BLAST of Lsi10G000950 vs. ExPASy TrEMBL
Match: A0A1S3C828 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 289/346 (83.53%), Postives = 313/346 (90.46%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPG-SDSSDLQDRYQNWMSKYGREYKSR 89
           MEAY+MIW+V L+SLILWVFWTP+ VSMAMDYP G S+S +LQDRYQ WM KYGR+YKSR
Sbjct: 1   MEAYKMIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSR 60

Query: 90  EERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLP--DTC 149
           EE E+RFTIYQ NVQYIDNFNSLNHSYTLAENNF DLTN+EF  TYLGY T  LP  DT 
Sbjct: 61  EEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTF 120

Query: 150 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQE 209
           FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIK GKLMSLSEQE
Sbjct: 121 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQE 180

Query: 210 LVDCDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGY 269
           LVDCDV SGNQGCNGGYMYKAFEF+KK+G+TTE EYPY  T S C+KQK +Y +V+I+GY
Sbjct: 181 LVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGY 240

Query: 270 EKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEAS 329
           EKVPVNDEKSL+AAVA QPVSVAIDAGG DFQFYSGG++SGNCGKQLNHGV+IVGYGE S
Sbjct: 241 EKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDS 300

Query: 330 SKPYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           ++ YWLVKNSWGT WGESGYIRM RDSTDK+GTCGIAMMASYPIKD
Sbjct: 301 NQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

[Close]

BLAST of Lsi10G000950 vs. ExPASy TrEMBL
Match: A0A0A0LJV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.8e-166
Identity = 282/340 (82.94%), Postives = 310/340 (91.18%), Query Frame = 0


Query: 35  MIW-NVGLMSLILWVFWTPSMVSMAMDYPPGSD-SSDLQDRYQNWMSKYGREYKSREERE 94
           M W NV L+ LILWVFWTP +VSMAMDY  GS  SSD+QDRYQ WM KYGR+YKSREE E
Sbjct: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60

Query: 95  RRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRYGNM 154
           RRFTIYQ NVQYIDNFNS+NHS+TLAENNFADLTN+EFK TYLGY+T  +PDTCFRYGNM
Sbjct: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120

Query: 155 VNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDV 214
           VNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGINKIK GKL+SLSEQELVDCDV
Sbjct: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180

Query: 215 VSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVN 274
            SGNQGCNGGYMYKAFEF+K++G+TTE EYPY+G ES CN+QK +Y  V+I+GYEKVPVN
Sbjct: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVN 240

Query: 275 DEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWL 334
           DEKSLKAAVANQPVSVAIDA G +FQFYSGG++SGNCG QLNHGV+IVGYGE S++ YWL
Sbjct: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300

Query: 335 VKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           VKNSWGTDWGESGYIRMKRDSTD++GTCGIAMMASYP KD
Sbjct: 301 VKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]

BLAST of Lsi10G000950 vs. ExPASy TrEMBL
Match: A0A6J1CH04 (ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.7e-165
Identity = 282/343 (82.22%), Postives = 306/343 (89.21%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSRE 89
           MEAY MI NVG M LIL VFWT SM S+A D PPG  S D++DRYQ W+ KYGREYKS E
Sbjct: 1   MEAYGMIRNVGFMWLILCVFWTLSMASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGE 60

Query: 90  ERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRY 149
           ERE+RF IYQ NVQYID FNSLN SYTLA+N FADLTNDEFKTTYLGY TDW PDTCF+Y
Sbjct: 61  EREKRFPIYQSNVQYIDYFNSLNRSYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKY 120

Query: 150 GNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 209
           GN+VNLPTNVDWRKEGAVTPIK+QGQCGSCWAFSAVAAVEGI KIKTGKL+SLSEQEL+D
Sbjct: 121 GNIVNLPTNVDWRKEGAVTPIKDQGQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLD 180

Query: 210 CDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKV 269
           CDV+SGNQGC+GG+M KAFEF+KK GITTE EYPYRG E+VCNKQKVRYH+ TI+GYEKV
Sbjct: 181 CDVISGNQGCSGGFMPKAFEFIKKIGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKV 240

Query: 270 PVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKP 329
           P NDEKSLKAAVANQPVSVAIDAGGYDFQFYSGG++SGNCGKQLNHGV+IVGYGE   K 
Sbjct: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGKS 300

Query: 330 YWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           YWLVKNSWGT WGE GY+RMK +S+DKRGTCGIAM ASYPIKD
Sbjct: 301 YWLVKNSWGTSWGEYGYVRMKSNSSDKRGTCGIAMDASYPIKD 343

[Close]

BLAST of Lsi10G000950 vs. NCBI nr
Match: XP_038902939.1 (ervatamin-B [Benincasa hispida])

HSP 1 Score: 658.3 bits (1697), Expect = 3.9e-185
Identity = 311/343 (90.67%), Postives = 327/343 (95.34%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSRE 89
           MEAYRMIWNVGLMSLILWV WTP+MV MAMDYPPGS S DLQ RYQ WMSKYGR+YKSRE
Sbjct: 1   MEAYRMIWNVGLMSLILWVIWTPTMVFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSRE 60

Query: 90  ERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRY 149
           E ERRFTIYQLNVQYIDNFNSL+HSYTLAENNFADLTNDEFK TYLGY+TDWLPDTCFRY
Sbjct: 61  EWERRFTIYQLNVQYIDNFNSLDHSYTLAENNFADLTNDEFKETYLGYKTDWLPDTCFRY 120

Query: 150 GNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 209
           GNMV+LPTNV+WRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVD
Sbjct: 121 GNMVDLPTNVNWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 180

Query: 210 CDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKV 269
           CDV SGNQGCNGG+M KAF+F+KK+G+TTETEYPYRG ES CNKQKVR HTV I+GYEKV
Sbjct: 181 CDVASGNQGCNGGFMDKAFQFIKKTGLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKV 240

Query: 270 PVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKP 329
           P NDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGV+SGNCGKQLNHGV+IVGYG+AS+K 
Sbjct: 241 PANDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYGQASNKS 300

Query: 330 YWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           YWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD
Sbjct: 301 YWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 343

[Close]

BLAST of Lsi10G000950 vs. NCBI nr
Match: XP_038902648.1 (ervatamin-B-like [Benincasa hispida])

HSP 1 Score: 629.4 bits (1622), Expect = 2.0e-176
Identity = 299/344 (86.92%), Postives = 318/344 (92.44%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSRE 89
           MEAYRMIWNVGLMSLILWV WTP+M  MAMDYPPGS S DLQ RYQ WMSKYGR+YKSRE
Sbjct: 1   MEAYRMIWNVGLMSLILWVIWTPTMEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSRE 60

Query: 90  ERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRY 149
           E ERRFTIYQLNVQYIDNFNSL+HSYTLAENN  DLTNDEFK TYLGY+TDWLPDTCFRY
Sbjct: 61  EWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRY 120

Query: 150 GNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 209
           GNMV+LPTNV+WRKEGAVTPI NQGQCG+CWAFSAVAAVEGINKIKTGKLMSLSEQELVD
Sbjct: 121 GNMVHLPTNVNWRKEGAVTPINNQGQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVD 180

Query: 210 CDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKV 269
           CDV SGNQGCNGG+M KAF+F+KK+ +TTETEYPYRG ES CNKQKVR HTV I+GYEKV
Sbjct: 181 CDVASGNQGCNGGFMDKAFQFIKKTRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKV 240

Query: 270 PVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEAS-SK 329
           P NDEKSLKA VANQPVS+AIDAGGYDFQFYSGGV+SGNCGKQLNHGV+IVGY +A+  K
Sbjct: 241 PANDEKSLKAVVANQPVSLAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQATLHK 300

Query: 330 PYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
            YWLVKNSWGT+WGESGYIRMK DSTDKRGTCGIAMMASYPIKD
Sbjct: 301 SYWLVKNSWGTNWGESGYIRMKSDSTDKRGTCGIAMMASYPIKD 344

[Close]

BLAST of Lsi10G000950 vs. NCBI nr
Match: AKO60151.1 (cysteine proteinase 1, partial [Citrullus lanatus])

HSP 1 Score: 613.6 bits (1581), Expect = 1.1e-171
Identity = 291/319 (91.22%), Postives = 304/319 (95.30%), Query Frame = 0


Query: 54  MVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNH 113
           M SM MDY PGS S DLQDRYQ WMSKYGREYKSREE E+RF IYQLNVQYIDNFNSLNH
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 114 SYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRYGNMVNLPTNVDWRKEGAVTPIKNQ 173
           SYTLAEN+FADLTNDEFKTTYLG++TDWLPDT FRYGNMVNLPTNVDWRKE AVTP+K+Q
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 174 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVKK 233
           GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDV SGNQGCNGGYMYKAFEF+KK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 234 SGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAG 293
           +G+TTE EYPYRG ESVCNKQKVRY TVTI+GYEKVPVNDEKSLKAAVANQPVSVAIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 294 GYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWLVKNSWGTDWGESGYIRMKRDS 353
           GYDFQFYSGGV+SGNCGKQLNHGV+IVGYGEAS+K YWLVKNSWGTDWGESGYIRMKRDS
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNKTYWLVKNSWGTDWGESGYIRMKRDS 300

Query: 354 TDKRGTCGIAMMASYPIKD 373
           TDKRGTCGIAMMASYPIKD
Sbjct: 301 TDKRGTCGIAMMASYPIKD 319

[Close]

BLAST of Lsi10G000950 vs. NCBI nr
Match: XP_008458487.1 (PREDICTED: ervatamin-B-like [Cucumis melo] >KAA0033464.1 ervatamin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 601.7 bits (1550), Expect = 4.4e-168
Identity = 289/346 (83.53%), Postives = 313/346 (90.46%), Query Frame = 0


Query: 30  MEAYRMIWNVGLMSLILWVFWTPSMVSMAMDYPPG-SDSSDLQDRYQNWMSKYGREYKSR 89
           MEAY+MIW+V L+SLILWVFWTP+ VSMAMDYP G S+S +LQDRYQ WM KYGR+YKSR
Sbjct: 1   MEAYKMIWSVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSR 60

Query: 90  EERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLP--DTC 149
           EE E+RFTIYQ NVQYIDNFNSLNHSYTLAENNF DLTN+EF  TYLGY T  LP  DT 
Sbjct: 61  EEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTF 120

Query: 150 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQE 209
           FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIK GKLMSLSEQE
Sbjct: 121 FRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQE 180

Query: 210 LVDCDVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGY 269
           LVDCDV SGNQGCNGGYMYKAFEF+KK+G+TTE EYPY  T S C+KQK +Y +V+I+GY
Sbjct: 181 LVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGY 240

Query: 270 EKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEAS 329
           EKVPVNDEKSL+AAVA QPVSVAIDAGG DFQFYSGG++SGNCGKQLNHGV+IVGYGE S
Sbjct: 241 EKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDS 300

Query: 330 SKPYWLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           ++ YWLVKNSWGT WGESGYIRM RDSTDK+GTCGIAMMASYPIKD
Sbjct: 301 NQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

[Close]

BLAST of Lsi10G000950 vs. NCBI nr
Match: XP_004148072.2 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 597.0 bits (1538), Expect = 1.1e-166
Identity = 283/342 (82.75%), Postives = 312/342 (91.23%), Query Frame = 0


Query: 33  YRMIW-NVGLMSLILWVFWTPSMVSMAMDYPPGSD-SSDLQDRYQNWMSKYGREYKSREE 92
           Y+M W NV L+ LILWVFWTP +VSMAMDY  GS  SSD+QDRYQ WM KYGR+YKSREE
Sbjct: 15  YKMTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREE 74

Query: 93  RERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWLPDTCFRYG 152
            ERRFTIYQ NVQYIDNFNS+NHS+TLAENNFADLTN+EFK TYLGY+T  +PDTCFRYG
Sbjct: 75  WERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYG 134

Query: 153 NMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDC 212
           NMVNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGINKIK GKL+SLSEQELVDC
Sbjct: 135 NMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDC 194

Query: 213 DVVSGNQGCNGGYMYKAFEFVKKSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVP 272
           DV SGNQGCNGGYMYKAFEF+K++G+TTE EYPY+G ES CN+QK +Y  V+I+GYEKVP
Sbjct: 195 DVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVP 254

Query: 273 VNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKPY 332
           VNDEKSLKAAVANQPVSVAIDA G +FQFYSGG++SGNCG QLNHGV+IVGYGE S++ Y
Sbjct: 255 VNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAY 314

Query: 333 WLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIKD 373
           WLVKNSWGTDWGESGYIRMKRDSTD++GTCGIAMMASYP KD
Sbjct: 315 WLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 356

[Close]

BLAST of Lsi10G000950 vs. TAIR 10
Match: AT1G06260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 9.0e-95
Identity = 179/341 (52.49%), Postives = 227/341 (66.57%), Query Frame = 0


Query: 38  NVGLMSLILWVFWTPSMVSMAMDYPPGSDSSDLQDRYQNWMSKYGREYKSREERERRFTI 97
           N+ L  LI +V     + S  +D         L+ R++ W+  + + Y  R+E   RF I
Sbjct: 9   NLTLAVLICFVLIASKLCS--VDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGI 68

Query: 98  YQLNVQYIDNFNSLNHSYTLAENNFADLTNDEFKTTYLGYRTDWL------PDTCFRYGN 157
           YQ NVQ ID  NSL+  + L +N FAD+TN EFK  +LG  T  L         C   G 
Sbjct: 69  YQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAG- 128

Query: 158 MVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCD 217
             N+P  VDWR +GAVTPI+NQG+CG CWAFSAVAA+EGINKIKTG L+SLSEQ+L+DCD
Sbjct: 129 --NVPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCD 188

Query: 218 VVSGNQGCNGGYMYKAFEFVK-KSGITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVP 277
           V + N+GC+GG M  AFEF+K   G+ TET+YPY G E  C+++K +   VTI GY+KV 
Sbjct: 189 VGTYNKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTCDQEKSKNKVVTIQGYQKVA 248

Query: 278 VNDEKSLKAAVANQPVSVAIDAGGYDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASSKPY 337
            N E SL+ A A QPVSV IDAGG+ FQ YS GV++  CG  LNHGV++VGYG    + Y
Sbjct: 249 QN-EASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKY 308

Query: 338 WLVKNSWGTDWGESGYIRMKRDSTDKRGTCGIAMMASYPIK 372
           W+VKNSWGT WGE GYIRM+R  ++  G CGIAMMASYP++
Sbjct: 309 WIVKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMASYPLQ 343

[Close]

BLAST of Lsi10G000950 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 340.9 bits (873), Expect = 1.3e-93
Identity = 177/313 (56.55%), Postives = 215/313 (68.69%), Query Frame = 0


Query: 70  LQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTNDE 129
           L   Y  W S +    +S  ERE+RF +++ NV ++ N N  N SY L  N FADLT +E
Sbjct: 34  LSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINE 93

Query: 130 FKTTYLG-----YRTDWLP-----DTCFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSC 189
           FK  Y G     +R    P        + + N+  LP++VDWRK+GAVT IKNQG+CGSC
Sbjct: 94  FKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSC 153

Query: 190 WAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVKKS-GITT 249
           WAFS VAAVEGINKIKT KL+SLSEQELVDCD    N+GCNGG M  AFEF+KK+ GITT
Sbjct: 154 WAFSTVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCNGGLMEIAFEFIKKNGGITT 213

Query: 250 ETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQ 309
           E  YPY G +  C+  K     VTI G+E VP NDE +L  AVANQPVSVAIDAG  DFQ
Sbjct: 214 EDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQ 273

Query: 310 FYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWLVKNSWGTDWGESGYIRMKRDSTDKRG 369
           FYS GV++G+CG +LNHGV+ VGYG    K YW+V+NSWG +WGE GYI+++R+  +  G
Sbjct: 274 FYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEG 333

Query: 370 TCGIAMMASYPIK 372
            CGIAM ASYPIK
Sbjct: 334 RCGIAMEASYPIK 344

[Close]

BLAST of Lsi10G000950 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 338.2 bits (866), Expect = 8.4e-93
Identity = 167/316 (52.85%), Postives = 226/316 (71.52%), Query Frame = 0


Query: 70  LQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSL--NHSYTLAENNFADLTN 129
           +Q R+  WM+K+GR Y   +E   R+ +++ NV+ I++ NS+    ++ LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 130 DEFKTTYLGYR----------TDWLPDTCFRYGNMVN--LPTNVDWRKEGAVTPIKNQGQ 189
           DEF++ Y G++          T   P   FRY N+ +  LP +VDWRK+GAVTPIKNQG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSP---FRYQNVSSGALPVSVDWRKKGAVTPIKNQGS 153

Query: 190 CGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVKKS- 249
           CG CWAFSAVAA+EG  +IK GKL+SLSEQ+LVDCD  + + GC GG M  AFE +K + 
Sbjct: 154 CGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATG 213

Query: 250 GITTETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGG 309
           G+TTE+ YPY+G ++ CN +K      +ITGYE VPVNDE++L  AVA+QPVSV I+ GG
Sbjct: 214 GLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGG 273

Query: 310 YDFQFYSGGVYSGNCGKQLNHGVSIVGYGEASS-KPYWLVKNSWGTDWGESGYIRMKRDS 369
           +DFQFYS GV++G C   L+H V+ +GYGE+++   YW++KNSWGT WGESGY+R+++D 
Sbjct: 274 FDFQFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDV 333

[Close]

BLAST of Lsi10G000950 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.4e-92
Identity = 171/315 (54.29%), Postives = 216/315 (68.57%), Query Frame = 0


Query: 68  SDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFADLTN 127
           + L + Y+ W S +    +S EE+ +RF +++ NV++I   N  + SY L  N F D+T+
Sbjct: 32  NSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTS 91

Query: 128 DEFKTTYLG--------YRTDWLPDTCFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSC 187
           +EF+ TY G        ++ +      F Y N+  LPT+VDWRK GAVTP+KNQGQCGSC
Sbjct: 92  EEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSC 151

Query: 188 WAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEFVK-KSGITT 247
           WAFS V AVEGIN+I+T KL SLSEQELVDCD  + NQGCNGG M  AFEF+K K G+T+
Sbjct: 152 WAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTS 211

Query: 248 ETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQ 307
           E  YPY+ ++  C+  K     V+I G+E VP N E  L  AVANQPVSVAIDAGG DFQ
Sbjct: 212 ELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQ 271

Query: 308 FYSGGVYSGNCGKQLNHGVSIVGYGEA-SSKPYWLVKNSWGTDWGESGYIRMKRDSTDKR 367
           FYS GV++G CG +LNHGV++VGYG       YW+VKNSWG +WGE GYIRM+R    K 
Sbjct: 272 FYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKE 331

Query: 368 GTCGIAMMASYPIKD 373
           G CGIAM ASYP+K+
Sbjct: 332 GLCGIAMEASYPLKN 344

[Close]

BLAST of Lsi10G000950 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 333.6 bits (854), Expect = 2.1e-91
Identity = 165/313 (52.72%), Postives = 218/313 (69.65%), Query Frame = 0


Query: 65  SDSSDLQDRYQNWMSKYGREYKSREERERRFTIYQLNVQYIDNFNSLNHSYTLAENNFAD 124
           +++  L + +++WMS++ + YKS EE+  RF +++ N+ +ID  N+  +SY L  N FAD
Sbjct: 42  TNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFAD 101

Query: 125 LTNDEFKTTYLG-----YRTDWLPDTCFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSC 184
           LT++EFK  YLG     +     P   FRY ++ +LP +VDWRK+GAV P+K+QGQCGSC
Sbjct: 102 LTHEEFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSC 161

Query: 185 WAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVVSGNQGCNGGYMYKAFEF-VKKSGITT 244
           WAFS VAAVEGIN+I TG L SLSEQEL+DCD  + N GCNGG M  AF++ +   G+  
Sbjct: 162 WAFSTVAAVEGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHK 221

Query: 245 ETEYPYRGTESVCNKQKVRYHTVTITGYEKVPVNDEKSLKAAVANQPVSVAIDAGGYDFQ 304
           E +YPY   E +C +QK     VTI+GYE VP ND++SL  A+A+QPVSVAI+A G DFQ
Sbjct: 222 EDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQ 281

Query: 305 FYSGGVYSGNCGKQLNHGVSIVGYGEASSKPYWLVKNSWGTDWGESGYIRMKRDSTDKRG 364
           FY GGV++G CG  L+HGV+ VGYG +    Y +VKNSWG  WGE G+IRMKR++    G
Sbjct: 282 FYKGGVFNGKCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEG 341

Query: 365 TCGIAMMASYPIK 372
            CGI  MASYP K
Sbjct: 342 LCGINKMASYPTK 353

The following BLAST results are available for this feature:

BLAST of Lsi10G000950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P12412	1.5e-94	52.89	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
P25803	4.8e-93	50.99	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9STL4	1.8e-92	56.55	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]
Q7XWK5	1.8e-92	54.29	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. japonica OX=3...	[more]
A2XQE8	2.4e-92	54.60	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of Lsi10G000950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A384S0D9	5.4e-172	91.22	Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1	[more]
A0A5A7SQK0	2.1e-168	83.53	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001...	[more]
A0A1S3C828	2.1e-168	83.53	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1	[more]
A0A0A0LJV6	5.8e-166	82.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1	[more]
A0A6J1CH04	1.7e-165	82.22	ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1	[more]

BLAST of Lsi10G000950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902939.1	3.9e-185	90.67	ervatamin-B [Benincasa hispida]	[more]
XP_038902648.1	2.0e-176	86.92	ervatamin-B-like [Benincasa hispida]	[more]
AKO60151.1	1.1e-171	91.22	cysteine proteinase 1, partial [Citrullus lanatus]	[more]
XP_008458487.1	4.4e-168	83.53	PREDICTED: ervatamin-B-like [Cucumis melo] >KAA0033464.1 ervatamin-B-like [Cucum...	[more]
XP_004148072.2	1.1e-166	82.75	ervatamin-B [Cucumis sativus]	[more]

BLAST of Lsi10G000950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G06260.1	9.0e-95	52.49	Cysteine proteinases superfamily protein	[more]
AT3G48340.1	1.3e-93	56.55	Cysteine proteinases superfamily protein	[more]
AT5G45890.1	8.4e-93	52.85	senescence-associated gene 12	[more]
AT5G50260.1	1.4e-92	54.29	Cysteine proteinases superfamily protein	[more]
AT4G35350.1	2.1e-91	52.72	xylem cysteine peptidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 173..188 score: 67.03 coord: 330..336 score: 75.76 coord: 315..325 score: 54.04
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 155..370 e-value: 1.3E-122 score: 423.4
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 155..370 e-value: 2.0E-84 score: 283.1
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 74..130 e-value: 1.4E-21 score: 87.7
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 74..130 e-value: 7.9E-15 score: 55.0
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 58..372 e-value: 3.6E-121 score: 406.5
None	No IPR available	PANTHER	PTHR12411:SF796	ERVATAMIN-B-LIKE	coord: 59..371
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 59..371
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 330..349
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 173..184
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 156..369 e-value: 1.73568E-112 score: 324.962
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 70..371

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G000950.1	Lsi10G000950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity