Lsi10G000660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G000660
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRab-GAP TBC domain-containing protein
Locationchr10: 1108591 .. 1112345 (-)
RNA-Seq ExpressionLsi10G000660
SyntenyLsi10G000660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCTCCTTTTGGTTAATTTCAAAACCCACCTCACAGGCTGAGGCTGAGGCAGAGGCTCTATCTATTGGCTTTGCTTCCATTTATTCTCAGCTTTTTTTCCCCTTAAAAATCCCGATTTTCTTGTGCCCTTTCAACGGCTCAACTCCTCCATTTTTGTCATCCATTCCAAAAATGATTTTTAATTCAATTTTTATATCATTCAATCCCAACCCTTCATCACCTCTGCTATAATCACCATATAAATTCTCTACTTTGTGTTTCCTGGTTTCGTTTTTTACCTTAACCTTTACCTGCTTGTCTCTCTGTTTGTAATTTCTTTTTGGGGAAGAATCGATTTCTTCCCATTTTGTTTTTCTTTTTTAAAAATTAATGTAAGAGCCGACTCCAGAAGCCAATAAATTAAAGATTTTTTATGGCGGATTTGATGGCAATGGTGGGGAGTTTTTGGAGAACATTTCTTCTTGTGTAGGTGTAGCTTTTCTTCGTGGAAGTCTTGGGGATAAGACCCTTTTTTGTGAAACTCAGGTACGCAGCCTTCTAAAGCTCCAAAATAGACCAATTCTCATGGTTTGAAGCTGGTGGGGTTTCTTTATTTGACGAAATCATCTAACCCTTTTGATATTAAAGAACCAAAACCACGAAATCCCCTTCTTTTTTTCTTTTCTTGATCTAATTTGGAATAACCCCAAGAAATGTTTGGGACTCAAAGCAAAAGAGATATTGCTCTAGAATTGCAAGCTCAGATTCCAATTCTAAGGCCGAGTATCCACGCGAGAAGGGCAAATATTATAGTTAAGTTTCAAGATCTATATGGGTTCACAGTGGAAGGCAATGTGGATGATGTTAATGTGTTGAATGAAGTGAGGGAAAAGGTTAGACAACAGGGTCGTGTTTGGTGGGCATTGGAGGCTAGCAAAGGCGCTAATTGGTACTTGGAACCCTCTGTTTCTGAAGGGATCGCCCTCAAATCCTCCCTCAAATTATCAACCCTTGCTAATGCCATCACTTTGAAGAAGTTGATTAGGAAAGGAATTCCGCCGGTTCTTCGTCCCAAGGTTTGGTTCTCCTTATCCGGAGCTGCCAAGAAGAAATCCACGGTCCCCGATAGCTATTACAATGATTTGACCAAGGCCGTCGAAGGGAAAGTCACGCCGGCCACTAGGCAGATTGATCATGTATGTTCTTTTCGAATGATCCTTGATCTTCGATGACTATGCACTTTGCAGCTATAATTCGATTCTTCCTTTGAGAATTCTATTGAGGATTTTGTATGCTTTGATTGTATGAGTTTTCGATTCTCATTGAAAATGAATATCAGAATTGCATTTCATCTTGACCATTAGGAGGGGTGCATCTTTTGTGATTTAGCTTTGGATTAAATATTTGAGTAGTTAAGGGACCTCAATTGTCTTTAATCTACTTAACAGGGCTAAAATTGATGAGACTAATAAATGGTGCAGATGGGATCTGGAGTTAAATTTGTTCTTTTGGTGGATGTGAATACAAATTTAACGTTAATGAGCTTGATTGACTCTCATAATATTTGGATGATAATAAATTGTATGTTAGTTATTGTTGTTGGTTGCTATTTCTGTAATTCATAGTTTCAATATGTGTGTGTATATATATATATTTCTTTGAGTTATTGATTTGTTCTTGTAACCATAAGATGCATGCCCTCATTGTGATTTAATTTATCCTTCCGTTCATCTACATTGGGTAAAATCATTGGAAAACCTTAGGTGCAGATGGTGTCTGGTATAATTTCTCTCTTAATCTATAAATGATTGGAGGTCCGTTCACTTTATTTTATGTTGCTTTCAATCTTTTCAACTTGTTCCTGTGTTTTTTCTTATACACATGCAGGACTTGCCCCGTACTTTCCCTGGTCATCCTTGGTTAGACACTCCAGCGGGTCACGCCGCTCTTCGACGTGTTCTTGTTGGGTATTCCTTCCGCGACTCTGATGTTGGATACTGTCAGGTGGGTTTGTTTTTCTCCTTCCCTCTAACATAACTTTCTGCTCAGTAACTTAGCATAATGTTAGGACGGGTTTTAACCCCTATACTTTACTCGTAGTCTGCGTAATATGCATTCGGTATTCCACATCCTTTCTTCTCAGTCTAAAACTGGGATTCAACTCTGTTTCTCATCAGTTGCTGCAGTGGTCTATCCATGCCTAATTCATCTCTCTCCTTCTATCCCCGTCCTCCTCATCCTGTTGTTTCTCTAGAATACTGATTCAGAGTATGGAAATGATTTCTAGTTGCTATTTGTAAAAGACATGGTTATCACATACTATTGGTATTTGATCTTTTTGTGGCTGAATTAGACCTGTAATTTATAGAACACTTTGGAATTAATTTAGTTTTGGTGCAGGGCTTAAACTATGTTGCAGCATTGTTATTGCTCGTGATGAAAACAGAAGAAGACGCTTTTTGGATGCTAGCTGTCCTCTTAGAAAATGTTTTAGTCAGTGACTGTTACACGACCAACTTGTCAGGATGTCATGTCGAGCAACGGGTATTCAAAGATTTACTTACCAAAAAGTGTCCCAGGTGCCTCACTTTGTTTTGACATTCATTTATTGTCTAATAAGTACAAGCATTTTTATAAATTGGAGTGTAAATGCAGGATAGCTGCACATTTGGAAGCATTGGATTTTGATGTCTCCCTTGTCGCCACTGAGTGGTTCCTGTGTCTTTTCTCTAAGAGCCTACCTTCTGAGGTTAGAACTATGCTCTCTCTTGGTGCCCATGCTTGATTTTGTTTTGTTTTTACAAGAAAAAGAAAACTAAATTCCGTTACAACAATGTCGAACCACTTAATTCTTAAGCATTCAAACACCAGAAAATGTAGATTTTATACCCCTTAGCTGCAACGATATGATATGGAATAAGGGGAAATATCCAGTTTCTGGCCAAATGGAACCCAAACTTGAAAGTCATCCTTCAACATTGTCCATCTTTTTGAGCAGTAGTGCTAAAACAGCCGAGAGTTATACAAGTCTATCTGATGTGTATATATGCATAGATGTTTGTAGTAGAAAACTCTCTAAACCATGTTTTAGTTATTGACGAGACTGAAACATATACATGTTTGGGATCTTTTTCACAGACGACTCTACGTGTTTGGGACGTCCTTTTCTATGAGGGAGCAAAAGTTCTGTTTCATGTGGCTTTGGCTATCTTTAAGGTAATCGCTTGGAACTTGTAATCTATCGATGGTAGAGATTTCTGTTTCCACCATTTCTTGAAAGTCAGATCAATGGCCATGCTTGATCTTTTGAGCACATTGTGATTATATCTATATTTGCGATTTCAGATGAAGGAAGGGGAGTTGCTTATAAGTCATCATGTGGGAGATGTGATTAATATTCTACAAAAAACTACACATAACCTTTTCGATCCCGATGATCTACTGACGGTAACTGTCTTTCCATTACATCTTTGCAAAGTTCAATTTATATTCAACTTGACATTGTTGTAGACTGCCTCTTCACATTGTAACTTTCCGTGGTTTGAAGCTTCGACTTGCCAATTCATGTTTTTAGATATCGAATTAGTCCCACTTTTTTTCCGATGACGGTTTGAAAGAAAGCTTTTGGATTGTAAACTTGCAGGTAGCATATGACAAAATTGGATCAATGACTACCAACACTATATCAAAGCAAAGGAAAAAGCAAGAACCAGCAGTTATGGCTGAGCTTGATCAGAGATTGAGGCGACTAAATTCCCTTAAAGCGGATGACAAA

mRNA sequence

CCTCTCCTTTTGGTTAATTTCAAAACCCACCTCACAGGCTGAGGCTGAGGCAGAGGCTCTATCTATTGGCTTTGCTTCCATTTATTCTCAGCTTTTTTTCCCCTTAAAAATCCCGATTTTCTTGTGCCCTTTCAACGGCTCAACTCCTCCATTTTTGTCATCCATTCCAAAAATGATTTTTAATTCAATTTTTATATCATTCAATCCCAACCCTTCATCACCTCTGCTATAATCACCATATAAATTCTCTACTTTGTGTTTCCTGGTTTCGTTTTTTACCTTAACCTTTACCTGCTTGTCTCTCTGTTTGTAATTTCTTTTTGGGGAAGAATCGATTTCTTCCCATTTTGTTTTTCTTTTTTAAAAATTAATGTAAGAGCCGACTCCAGAAGCCAATAAATTAAAGATTTTTTATGGCGGATTTGATGGCAATGGTGGGGAGTTTTTGGAGAACATTTCTTCTTGTGTAGGTGTAGCTTTTCTTCGTGGAAGTCTTGGGGATAAGACCCTTTTTTGTGAAACTCAGGTACGCAGCCTTCTAAAGCTCCAAAATAGACCAATTCTCATGGTTTGAAGCTGGTGGGGTTTCTTTATTTGACGAAATCATCTAACCCTTTTGATATTAAAGAACCAAAACCACGAAATCCCCTTCTTTTTTTCTTTTCTTGATCTAATTTGGAATAACCCCAAGAAATGTTTGGGACTCAAAGCAAAAGAGATATTGCTCTAGAATTGCAAGCTCAGATTCCAATTCTAAGGCCGAGTATCCACGCGAGAAGGGCAAATATTATAGTTAAGTTTCAAGATCTATATGGGTTCACAGTGGAAGGCAATGTGGATGATGTTAATGTGTTGAATGAAGTGAGGGAAAAGGTTAGACAACAGGGTCGTGTTTGGTGGGCATTGGAGGCTAGCAAAGGCGCTAATTGGTACTTGGAACCCTCTGTTTCTGAAGGGATCGCCCTCAAATCCTCCCTCAAATTATCAACCCTTGCTAATGCCATCACTTTGAAGAAGTTGATTAGGAAAGGAATTCCGCCGGTTCTTCGTCCCAAGGTTTGGTTCTCCTTATCCGGAGCTGCCAAGAAGAAATCCACGGTCCCCGATAGCTATTACAATGATTTGACCAAGGCCGTCGAAGGGAAAGTCACGCCGGCCACTAGGCAGATTGATCATGACTTGCCCCGTACTTTCCCTGGTCATCCTTGGTTAGACACTCCAGCGGGTCACGCCGCTCTTCGACGTGTTCTTGTTGGGTATTCCTTCCGCGACTCTGATGTTGGATACTGTCAGCATAATGTTAGGACGGGTTTTAACCCCTATACTTTACTCGTAGTCTGCGTAATATGCATTCGGTATTCCACATCCTTTCTTCTCAGTCTAAAACTGGGATTCAACTCTGTTTCTCATCATGGTCTATCCATGCCTAATTCATCTCTCTCCTTCTATCCCCGTCCTCCTCATCCTGTTGGCTTAAACTATGTTGCAGCATTGTTATTGCTCGTGATGAAAACAGAAGAAGACGCTTTTTGGATGCTAGCTGTCCTCTTAGAAAATTCAGTGACTGTTACACGACCAACTTGTCAGGATGTCATGTCGAGCAACGGGTATTCAAAGATTTACTTACCAAAAAGTGTCCCAGGTGCCTCACTTTGTTTTGACATTCATTTATTGTCTAATAAGTACAAGCATTTTTATAAATTGGAGTGTAAATGCAGGATAGCTGCACATTTGGAAGCATTGGATTTTGATGTCTCCCTTGTCGCCACTGAGTGGTTCCTGTGTCTTTTCTCTAAGAGCCTACCTTCTGAGACGACTCTACGTGTTTGGGACGTCCTTTTCTATGAGGGAGCAAAAGTTCTGTTTCATGTGGCTTTGGCTATCTTTAAGATGAAGGAAGGGGAGTTGCTTATAAGTCATCATGTGGGAGATGTGATTAATATTCTACAAAAAACTACACATAACCTTTTCGATCCCGATGATCTACTGACGTCCCACTTTTTTTCCGATGACGGTTTGAAAGAAAGCTTTTGGATTGTAAACTTGCAGGTAGCATATGACAAAATTGGATCAATGACTACCAACACTATATCAAAGCAAAGGAAAAAGCAAGAACCAGCAGTTATGGCTGAGCTTGATCAGAGATTGAGGCGACTAAATTCCCTTAAAGCGGATGACAAA

Coding sequence (CDS)

ATGTTTGGGACTCAAAGCAAAAGAGATATTGCTCTAGAATTGCAAGCTCAGATTCCAATTCTAAGGCCGAGTATCCACGCGAGAAGGGCAAATATTATAGTTAAGTTTCAAGATCTATATGGGTTCACAGTGGAAGGCAATGTGGATGATGTTAATGTGTTGAATGAAGTGAGGGAAAAGGTTAGACAACAGGGTCGTGTTTGGTGGGCATTGGAGGCTAGCAAAGGCGCTAATTGGTACTTGGAACCCTCTGTTTCTGAAGGGATCGCCCTCAAATCCTCCCTCAAATTATCAACCCTTGCTAATGCCATCACTTTGAAGAAGTTGATTAGGAAAGGAATTCCGCCGGTTCTTCGTCCCAAGGTTTGGTTCTCCTTATCCGGAGCTGCCAAGAAGAAATCCACGGTCCCCGATAGCTATTACAATGATTTGACCAAGGCCGTCGAAGGGAAAGTCACGCCGGCCACTAGGCAGATTGATCATGACTTGCCCCGTACTTTCCCTGGTCATCCTTGGTTAGACACTCCAGCGGGTCACGCCGCTCTTCGACGTGTTCTTGTTGGGTATTCCTTCCGCGACTCTGATGTTGGATACTGTCAGCATAATGTTAGGACGGGTTTTAACCCCTATACTTTACTCGTAGTCTGCGTAATATGCATTCGGTATTCCACATCCTTTCTTCTCAGTCTAAAACTGGGATTCAACTCTGTTTCTCATCATGGTCTATCCATGCCTAATTCATCTCTCTCCTTCTATCCCCGTCCTCCTCATCCTGTTGGCTTAAACTATGTTGCAGCATTGTTATTGCTCGTGATGAAAACAGAAGAAGACGCTTTTTGGATGCTAGCTGTCCTCTTAGAAAATTCAGTGACTGTTACACGACCAACTTGTCAGGATGTCATGTCGAGCAACGGGTATTCAAAGATTTACTTACCAAAAAGTGTCCCAGGTGCCTCACTTTGTTTTGACATTCATTTATTGTCTAATAAGTACAAGCATTTTTATAAATTGGAGTGTAAATGCAGGATAGCTGCACATTTGGAAGCATTGGATTTTGATGTCTCCCTTGTCGCCACTGAGTGGTTCCTGTGTCTTTTCTCTAAGAGCCTACCTTCTGAGACGACTCTACGTGTTTGGGACGTCCTTTTCTATGAGGGAGCAAAAGTTCTGTTTCATGTGGCTTTGGCTATCTTTAAGATGAAGGAAGGGGAGTTGCTTATAAGTCATCATGTGGGAGATGTGATTAATATTCTACAAAAAACTACACATAACCTTTTCGATCCCGATGATCTACTGACGTCCCACTTTTTTTCCGATGACGGTTTGAAAGAAAGCTTTTGGATTGTAAACTTGCAGGTAGCATATGACAAAATTGGATCAATGACTACCAACACTATATCAAAGCAAAGGAAAAAGCAAGAACCAGCAGTTATGGCTGAGCTTGATCAGAGATTGAGGCGACTAAATTCCCTTAAAGCGGATGACAAA

Protein sequence

MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREKVRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQKTTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAELDQRLRRLNSLKADDK
Homology
BLAST of Lsi10G000660 vs. ExPASy Swiss-Prot
Match: Q6ZT07 (TBC1 domain family member 9 OS=Homo sapiens OX=9606 GN=TBC1D9 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 1.2e-25
Identity = 106/390 (27.18%), Postives = 164/390 (42.05%), Query Frame = 0

Query: 107 KKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRT 166
           ++L+ KGIP  +R ++W  LSGA  +K+T P  YY DL +   GK   AT +I+ DL R+
Sbjct: 509 RELVLKGIPESMRGELWLLLSGAINEKATHP-GYYEDLVEKSMGKYNLATEEIERDLHRS 568

Query: 167 FPGHPWLDTPAGHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSF 226
            P HP      G AALRRVL  Y+FR+ ++GYCQ                          
Sbjct: 569 LPEHPAFQNEMGIAALRRVLTAYAFRNPNIGYCQ-------------------------- 628

Query: 227 LLSLKLGFNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLL 286
                                             +N V ++LLL  K EE+AFW+L  L 
Sbjct: 629 ---------------------------------AMNIVTSVLLLYAK-EEEAFWLLVALC 688

Query: 287 ENSVTVTRPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAH 346
           E  +                   Y    V GA +  D  +     + +      C     
Sbjct: 689 ERML-----------------PDYYNTRVVGALV--DQGVFEELARDYVPQLYDC----- 748

Query: 347 LEALDFDVSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELL 406
           ++ L   +S ++  WFL LF   +P E+ + V D  FYEG KV+F +ALA+      +LL
Sbjct: 749 MQDLGV-ISTISLSWFLTLFLSVMPFESAVVVVDCFFYEGIKVIFQLALAVLDANVDKLL 808

Query: 407 ISHHVGDVINILQKTTHNLFDPDDLL-----TSHFFSDDGLKESFWIVN----LQVAYDK 466
                G+ + +L +   ++ + D  L          SDD   E +  V+    ++ +Y+K
Sbjct: 809 NCKDDGEAMTVLGRYLDSVTNKDSTLPPIPHLHSLLSDD--VEPYPEVDIFRLIRTSYEK 810

Query: 467 IGSMTTNTISKQRKKQEPAVMAELDQRLRR 488
            G++  + I + R KQ   V+  L+   +R
Sbjct: 869 FGTIRADLIEQMRFKQRLKVIQTLEDTTKR 810

BLAST of Lsi10G000660 vs. ExPASy Swiss-Prot
Match: Q9BYX2 (TBC1 domain family member 2A OS=Homo sapiens OX=9606 GN=TBC1D2 PE=1 SV=3)

HSP 1 Score: 118.6 bits (296), Expect = 2.0e-25
Identity = 100/342 (29.24%), Postives = 140/342 (40.94%), Query Frame = 0

Query: 97  LSTLANAITLKKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPAT 156
           L  L  +  LK+L+R G+P   RP+VW  L     +    P  Y   L++  + +  PA 
Sbjct: 609 LGDLVPSAELKQLLRAGVPREHRPRVWRWLVHLRVQHLHTPGCYQELLSRG-QAREHPAA 668

Query: 157 RQIDHDLPRTFPGHPWLDTPAGH--AALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLV 216
           RQI+ DL RTFP +     P       LRRVL+ +S+++  +GYCQ              
Sbjct: 669 RQIELDLNRTFPNNKHFTCPTSSFPDKLRRVLLAFSWQNPTIGYCQ-------------- 728

Query: 217 VCVICIRYSTSFLLSLKLGFNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKT 276
                                                        GLN +AA+ LLV++ 
Sbjct: 729 ---------------------------------------------GLNRLAAIALLVLEE 788

Query: 277 EEDAFWMLAVLLENSVTVTRPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHF 336
           EE AFW L  ++E            +M ++ Y        V    L     LLS K    
Sbjct: 789 EESAFWCLVAIVET-----------IMPADYYCNTLTASQVDQRVL---QDLLSEKLP-- 848

Query: 337 YKLECKCRIAAHLEALDFDVSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVA 396
                  R+ AHL     D+SLV   WFL +F+ SL S   LRVWD   YEG KV+F  A
Sbjct: 849 -------RLMAHLGQHHVDLSLVTFNWFLVVFADSLISNILLRVWDAFLYEGTKVVFRYA 867

Query: 397 LAIFKMKEGELLISHHVGDVINILQKTTHNLFDPDDLLTSHF 437
           LAIFK  E E+L   +  ++   L+  T  + +   L+   F
Sbjct: 909 LAIFKYNEKEILRLQNGLEIYQYLRFFTKTISNSRKLMNIAF 867

BLAST of Lsi10G000660 vs. ExPASy Swiss-Prot
Match: B1AVH7 (TBC1 domain family member 2A OS=Mus musculus OX=10090 GN=Tbc1d2 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.6e-25
Identity = 100/342 (29.24%), Postives = 139/342 (40.64%), Query Frame = 0

Query: 97  LSTLANAITLKKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPAT 156
           L+ L  +  LK+L+R G+P   RP+VW  L     +    P  Y   L +       PA 
Sbjct: 603 LTELTPSAELKQLLRAGVPREHRPRVWRWLVHRRVRHLQAPGCYQELLARG-RACEHPAA 662

Query: 157 RQIDHDLPRTFPGHPWLDTPAGH--AALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLV 216
           RQI+ DL RTFP +     P       LRRVL+ +S+++  +GYCQ              
Sbjct: 663 RQIELDLNRTFPTNKHFTCPTSSFPDKLRRVLLAFSWQNPTIGYCQ-------------- 722

Query: 217 VCVICIRYSTSFLLSLKLGFNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKT 276
                                                        GLN +AA+ LLV++ 
Sbjct: 723 ---------------------------------------------GLNRLAAIALLVLED 782

Query: 277 EEDAFWMLAVLLENSVTVTRPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHF 336
           EE AFW L  ++E            ++ +  YSK      V    L     LLS K    
Sbjct: 783 EESAFWCLVAIVET-----------ILPAEYYSKTLTASQVDQRVL---QDLLSEKLP-- 842

Query: 337 YKLECKCRIAAHLEALDFDVSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVA 396
                  R+ AHL     D+SL+   WFL +F+ SL S+  LRVWD   YEG KV+F  A
Sbjct: 843 -------RLTAHLGQHRVDLSLITFNWFLVVFADSLISDILLRVWDAFLYEGTKVVFRYA 861

Query: 397 LAIFKMKEGELLISHHVGDVINILQKTTHNLFDPDDLLTSHF 437
           LAIFK  E  +L      ++   L+  T  + D   L++  F
Sbjct: 903 LAIFKYNEEAILQLQDSLEIYQYLRFFTKTICDSRKLMSIAF 861

BLAST of Lsi10G000660 vs. ExPASy Swiss-Prot
Match: Q3UYK3 (TBC1 domain family member 9 OS=Mus musculus OX=10090 GN=Tbc1d9 PE=2 SV=2)

HSP 1 Score: 116.7 bits (291), Expect = 7.5e-25
Identity = 106/388 (27.32%), Postives = 161/388 (41.49%), Query Frame = 0

Query: 107 KKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRT 166
           ++L+ KGIP  +R ++W  LSGA  +K+T P  YY  L +   GK   AT +I+ DL R+
Sbjct: 509 RELVLKGIPESMRGELWLLLSGAINEKATHP-GYYEGLVEKSMGKYNLATEEIERDLHRS 568

Query: 167 FPGHPWLDTPAGHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSF 226
            P HP      G AALRRVL  Y+FR+ ++GYCQ                          
Sbjct: 569 LPEHPAFQNEMGIAALRRVLTAYAFRNPNIGYCQ-------------------------- 628

Query: 227 LLSLKLGFNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLL 286
                                             +N V ++LLL  K EE+AFW+L  L 
Sbjct: 629 ---------------------------------AMNIVTSVLLLYAK-EEEAFWLLVALC 688

Query: 287 ENSVTVTRPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAH 346
           E  +                   Y    V GA +  D  +     + +      C     
Sbjct: 689 ERML-----------------PDYYNTRVVGALV--DQGVFEELARDYVPQLYDC----- 748

Query: 347 LEALDFDVSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELL 406
           ++ L   +S ++  WFL LF   +P E+ + V D  FYEG KV+F +ALA+      +LL
Sbjct: 749 MQDLGV-ISTISLSWFLTLFLSVMPFESAVVVVDCFFYEGIKVIFQLALAVLDANVDKLL 808

Query: 407 ISHHVGDVINILQKTTHNLFDPDDLL-----TSHFFSDD-GLKESFWIVNL-QVAYDKIG 466
                G+ + +L +   ++ + D  L          SDD G   +  I  L   +Y+K G
Sbjct: 809 NCKDDGEAMTVLGRYLDSVTNKDSTLPPIPHLHSLLSDDVGPYPAVDIFRLIGTSYEKFG 810

Query: 467 SMTTNTISKQRKKQEPAVMAELDQRLRR 488
           ++  + I + R KQ   V+  L+   +R
Sbjct: 869 TIRADLIEQMRFKQRLKVIQTLEDTTKR 810

BLAST of Lsi10G000660 vs. ExPASy Swiss-Prot
Match: A6QP29 (TBC1 domain family member 2A OS=Bos taurus OX=9913 GN=TBC1D2 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.2e-24
Identity = 100/338 (29.59%), Postives = 138/338 (40.83%), Query Frame = 0

Query: 97  LSTLANAITLKKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPAT 156
           L  LA +  LK+L+R G+P   RP+VW  L     +    P  Y   L++    K + A 
Sbjct: 606 LGELAPSAELKQLLRAGVPHEHRPRVWRWLIRLRVQHLQAPGCYQALLSRGQACKHS-AA 665

Query: 157 RQIDHDLPRTFPGHPWLDTPAGH--AALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLV 216
           RQI+ DL RTFP +     P       LRRVL+ +S+++  +GYCQ              
Sbjct: 666 RQIELDLNRTFPNNKHFTCPTSSFPDKLRRVLLAFSWQNPTIGYCQ-------------- 725

Query: 217 VCVICIRYSTSFLLSLKLGFNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKT 276
                                                        GLN +AA+ LLV+  
Sbjct: 726 ---------------------------------------------GLNRLAAIALLVLDE 785

Query: 277 EEDAFWMLAVLLENSVTVTRPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHF 336
           EE AFW L  ++E            +M ++ YSK  L   V       D  +L +     
Sbjct: 786 EESAFWCLVAIVET-----------IMPADYYSKTLLASQV-------DQRVLQD----- 845

Query: 337 YKLECKCRIAAHLEALDFDVSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVA 396
             LE   R+ AHL     D+S +   WFL +F+ SL S   L+VWD   YEG KV+F  A
Sbjct: 846 LLLEKLPRLMAHLGQRHVDLSFITFNWFLVVFADSLISNILLQVWDAFLYEGIKVVFRYA 860

Query: 397 LAIFKMKEGELLISHHVGDVINILQKTTHNLFDPDDLL 433
           LAIFK  E  LL      ++   L   T  + D   L+
Sbjct: 906 LAIFKYNEEALLRLQDSLEIYQYLHFFTKTICDSRKLM 860

BLAST of Lsi10G000660 vs. ExPASy TrEMBL
Match: A0A5A7UC13 (TBC1 domain family member 2A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G00260 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 3.6e-187
Identity = 367/496 (73.99%), Postives = 376/496 (75.81%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVN+LNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNILNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLTKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLLTHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNSLKADDK
Sbjct: 481 LDQRLRRLNSLKADDK 395

BLAST of Lsi10G000660 vs. ExPASy TrEMBL
Match: A0A0A0LQ82 (Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G406820 PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.8e-186
Identity = 367/496 (73.99%), Postives = 375/496 (75.60%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYL+PSVSEGIALKSSLKLSTLANAITLKKLI KGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLQPSVSEGIALKSSLKLSTLANAITLKKLISKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLTKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLI+HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLITHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNSLKADDK
Sbjct: 481 LDQRLRRLNSLKADDK 395

BLAST of Lsi10G000660 vs. ExPASy TrEMBL
Match: A0A1S4E0V7 (small G protein signaling modulator 3 OS=Cucumis melo OX=3656 GN=LOC103496045 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 2.0e-185
Identity = 361/496 (72.78%), Postives = 369/496 (74.40%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVN+LNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNILNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ     GF     L        ++   +  LKL FNSVSH 
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ----VGFLSPPFL-------EHNNCSVTCLKLEFNSVSHQ 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
            L                                                          
Sbjct: 241 LLQC-------------------------------------------------------- 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
                            A LCFDIHLLS++YKHF KL  KCRIAAHLEALDFDVSLVATE
Sbjct: 301 -----------------ALLCFDIHLLSDEYKHFDKLGSKCRIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLLTHHVGDVINILQK 393

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 393

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNSLKADDK
Sbjct: 481 LDQRLRRLNSLKADDK 393

BLAST of Lsi10G000660 vs. ExPASy TrEMBL
Match: A0A0H4TH70 (TBC1 domain family member 2A OS=Momordica charantia OX=3673 GN=LOC111010987 PE=2 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.3e-184
Identity = 363/496 (73.19%), Postives = 374/496 (75.40%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           M+GTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MYGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VR+QGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRKQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGH WLDTPAGHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHTWLDTPAGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLSKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKE ELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEDELLLTHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNS+K DDK
Sbjct: 481 LDQRLRRLNSVKVDDK 395

BLAST of Lsi10G000660 vs. ExPASy TrEMBL
Match: A0A6J1G6E2 (growth hormone-regulated TBC protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451253 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 2.2e-181
Identity = 359/496 (72.38%), Postives = 372/496 (75.00%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIP+LRPSIHARRANI VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPVLRPSIHARRANITVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEA+KGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRQQGRVWWALEANKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVP+SYYNDLTKAVEGKVTPATRQIDHDLPRTFPGH WLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPESYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHAWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLTKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKE ELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEDELLVTHHVGDVINILQK 394

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 394

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNS + +DK
Sbjct: 481 LDQRLRRLNS-QVNDK 394

BLAST of Lsi10G000660 vs. NCBI nr
Match: KAA0053282.1 (TBC1 domain family member 2A [Cucumis melo var. makuwa] >TYK11875.1 TBC1 domain family member 2A [Cucumis melo var. makuwa])

HSP 1 Score: 664.5 bits (1713), Expect = 7.3e-187
Identity = 367/496 (73.99%), Postives = 376/496 (75.81%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVN+LNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNILNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLTKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLLTHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNSLKADDK
Sbjct: 481 LDQRLRRLNSLKADDK 395

BLAST of Lsi10G000660 vs. NCBI nr
Match: XP_038900967.1 (growth hormone-regulated TBC protein 1 [Benincasa hispida])

HSP 1 Score: 663.7 bits (1711), Expect = 1.3e-186
Identity = 367/496 (73.99%), Postives = 376/496 (75.81%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           M+GTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MYGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLTKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLI+HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLITHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNS+KADDK
Sbjct: 481 LDQRLRRLNSIKADDK 395

BLAST of Lsi10G000660 vs. NCBI nr
Match: XP_004138606.1 (growth hormone-regulated TBC protein 1 [Cucumis sativus] >XP_011649971.1 growth hormone-regulated TBC protein 1 [Cucumis sativus] >KGN63184.1 hypothetical protein Csa_022286 [Cucumis sativus])

HSP 1 Score: 662.1 bits (1707), Expect = 3.6e-186
Identity = 367/496 (73.99%), Postives = 375/496 (75.60%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYL+PSVSEGIALKSSLKLSTLANAITLKKLI KGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLQPSVSEGIALKSSLKLSTLANAITLKKLISKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLTKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLI+HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLITHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNSLKADDK
Sbjct: 481 LDQRLRRLNSLKADDK 395

BLAST of Lsi10G000660 vs. NCBI nr
Match: XP_016901858.1 (PREDICTED: small G protein signaling modulator 3 [Cucumis melo])

HSP 1 Score: 658.7 bits (1698), Expect = 4.0e-185
Identity = 361/496 (72.78%), Postives = 369/496 (74.40%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFGTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVN+LNEVREK
Sbjct: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNILNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTP GHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPEGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ     GF     L        ++   +  LKL FNSVSH 
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ----VGFLSPPFL-------EHNNCSVTCLKLEFNSVSHQ 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
            L                                                          
Sbjct: 241 LLQC-------------------------------------------------------- 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
                            A LCFDIHLLS++YKHF KL  KCRIAAHLEALDFDVSLVATE
Sbjct: 301 -----------------ALLCFDIHLLSDEYKHFDKLGSKCRIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLLTHHVGDVINILQK 393

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 393

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNSLKADDK
Sbjct: 481 LDQRLRRLNSLKADDK 393

BLAST of Lsi10G000660 vs. NCBI nr
Match: XP_022140274.1 (growth hormone-regulated TBC protein 1 [Momordica charantia] >AKQ06194.1 TBC1 domain family member 2A [Momordica charantia])

HSP 1 Score: 656.0 bits (1691), Expect = 2.6e-184
Identity = 363/496 (73.19%), Postives = 374/496 (75.40%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           M+GTQSKRDIALELQAQIPILRPSIHARRANI VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MYGTQSKRDIALELQAQIPILRPSIHARRANITVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120
           VR+QGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP
Sbjct: 61  VRKQGRVWWALEASKGANWYLEPSVSEGIALKSSLKLSTLANAITLKKLIRKGIPPVLRP 120

Query: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPAGHA 180
           KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGH WLDTPAGHA
Sbjct: 121 KVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHTWLDTPAGHA 180

Query: 181 ALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHH 240
           ALRRVLVGYSFRDSDVGYCQ                                        
Sbjct: 181 ALRRVLVGYSFRDSDVGYCQ---------------------------------------- 240

Query: 241 GLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDV 300
                              GLNYVAALLLLVMKTEEDAFWMLAVLLEN           V
Sbjct: 241 -------------------GLNYVAALLLLVMKTEEDAFWMLAVLLEN-----------V 300

Query: 301 MSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATE 360
           + S+ Y+           S C   H+    +K     +C  RIAAHLEALDFDVSLVATE
Sbjct: 301 LVSDCYT--------TNLSGC---HVEQRVFKDLLSKKCP-RIAAHLEALDFDVSLVATE 360

Query: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINILQK 420
           WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKE ELL++HHVGDVINILQK
Sbjct: 361 WFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEDELLLTHHVGDVINILQK 395

Query: 421 TTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAE 480
           TTH LFDPDDLLT                   VAYDKIGSMTTNTISKQRKKQEPAVMAE
Sbjct: 421 TTHYLFDPDDLLT-------------------VAYDKIGSMTTNTISKQRKKQEPAVMAE 395

Query: 481 LDQRLRRLNSLKADDK 497
           LDQRLRRLNS+K DDK
Sbjct: 481 LDQRLRRLNSVKVDDK 395

BLAST of Lsi10G000660 vs. TAIR 10
Match: AT3G07890.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 608.6 bits (1568), Expect = 4.5e-174
Identity = 335/499 (67.13%), Postives = 361/499 (72.34%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFG QS+RD+ +ELQ+QIPILRPSIHARRANI+VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MFGIQSRRDLTMELQSQIPILRPSIHARRANIVVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPS---VSEGIALKSSLKLSTLANAITLKKLIRKGIPPV 120
           VR QGRVWWALEASKGANWYL+P    + +GIALK+SLKLSTL NAITLK+LIRKGIPPV
Sbjct: 61  VRNQGRVWWALEASKGANWYLQPEILLIGDGIALKTSLKLSTLTNAITLKRLIRKGIPPV 120

Query: 121 LRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPA 180
           LRPKVWFSLSGAAKKKSTVP+SYY+DLTKAVEG VTPATRQIDHDLPRTFPGHPWLDTP 
Sbjct: 121 LRPKVWFSLSGAAKKKSTVPESYYSDLTKAVEGMVTPATRQIDHDLPRTFPGHPWLDTPE 180

Query: 181 GHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSV 240
           GHAALRRVLVGYSFRDSDVGYCQ                                     
Sbjct: 181 GHAALRRVLVGYSFRDSDVGYCQ------------------------------------- 240

Query: 241 SHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTC 300
                                 GLNYVAALLLLVMKTEEDAFWMLAVLLEN +       
Sbjct: 241 ----------------------GLNYVAALLLLVMKTEEDAFWMLAVLLENVLV------ 300

Query: 301 QDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLV 360
           +D  ++N              S C   H+    +K     +C  RIA HLE + FDVSLV
Sbjct: 301 RDCYTTN-------------LSGC---HVEQRVFKDLLAQKCS-RIATHLEDMGFDVSLV 360

Query: 361 ATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINI 420
           ATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFH ALAIFKMKE ELL++H VGDVINI
Sbjct: 361 ATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHAALAIFKMKENELLMTHQVGDVINI 398

Query: 421 LQKTTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAV 480
           LQKT+H LFDPD+LLT                   VA++KIGSMTTNTISKQRKKQEPAV
Sbjct: 421 LQKTSHQLFDPDELLT-------------------VAFEKIGSMTTNTISKQRKKQEPAV 398

Query: 481 MAELDQRLRRLNSLKADDK 497
           MAELDQRLRRLNSLK   K
Sbjct: 481 MAELDQRLRRLNSLKESGK 398

BLAST of Lsi10G000660 vs. TAIR 10
Match: AT3G07890.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 608.6 bits (1568), Expect = 4.5e-174
Identity = 335/499 (67.13%), Postives = 361/499 (72.34%), Query Frame = 0

Query: 1   MFGTQSKRDIALELQAQIPILRPSIHARRANIIVKFQDLYGFTVEGNVDDVNVLNEVREK 60
           MFG QS+RD+ +ELQ+QIPILRPSIHARRANI+VKFQDLYGFTVEGNVDDVNVLNEVREK
Sbjct: 1   MFGIQSRRDLTMELQSQIPILRPSIHARRANIVVKFQDLYGFTVEGNVDDVNVLNEVREK 60

Query: 61  VRQQGRVWWALEASKGANWYLEPS---VSEGIALKSSLKLSTLANAITLKKLIRKGIPPV 120
           VR QGRVWWALEASKGANWYL+P    + +GIALK+SLKLSTL NAITLK+LIRKGIPPV
Sbjct: 61  VRNQGRVWWALEASKGANWYLQPEILLIGDGIALKTSLKLSTLTNAITLKRLIRKGIPPV 120

Query: 121 LRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPATRQIDHDLPRTFPGHPWLDTPA 180
           LRPKVWFSLSGAAKKKSTVP+SYY+DLTKAVEG VTPATRQIDHDLPRTFPGHPWLDTP 
Sbjct: 121 LRPKVWFSLSGAAKKKSTVPESYYSDLTKAVEGMVTPATRQIDHDLPRTFPGHPWLDTPE 180

Query: 181 GHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSV 240
           GHAALRRVLVGYSFRDSDVGYCQ                                     
Sbjct: 181 GHAALRRVLVGYSFRDSDVGYCQ------------------------------------- 240

Query: 241 SHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTC 300
                                 GLNYVAALLLLVMKTEEDAFWMLAVLLEN +       
Sbjct: 241 ----------------------GLNYVAALLLLVMKTEEDAFWMLAVLLENVLV------ 300

Query: 301 QDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLV 360
           +D  ++N              S C   H+    +K     +C  RIA HLE + FDVSLV
Sbjct: 301 RDCYTTN-------------LSGC---HVEQRVFKDLLAQKCS-RIATHLEDMGFDVSLV 360

Query: 361 ATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHVALAIFKMKEGELLISHHVGDVINI 420
           ATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFH ALAIFKMKE ELL++H VGDVINI
Sbjct: 361 ATEWFLCLFSKSLPSETTLRVWDVLFYEGAKVLFHAALAIFKMKENELLMTHQVGDVINI 398

Query: 421 LQKTTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAV 480
           LQKT+H LFDPD+LLT                   VA++KIGSMTTNTISKQRKKQEPAV
Sbjct: 421 LQKTSHQLFDPDELLT-------------------VAFEKIGSMTTNTISKQRKKQEPAV 398

Query: 481 MAELDQRLRRLNSLKADDK 497
           MAELDQRLRRLNSLK   K
Sbjct: 481 MAELDQRLRRLNSLKESGK 398

BLAST of Lsi10G000660 vs. TAIR 10
Match: AT3G55020.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.2e-25
Identity = 113/419 (26.97%), Postives = 167/419 (39.86%), Query Frame = 0

Query: 97  LSTLANAITLKKLIRKGIPPVLRPKVWFSLSGAAKKKSTVPDSYYNDLTKAVEGKVTPAT 156
           LST      L+ LIR G+P  LR ++W +  G  K++      YY +L  A +G V    
Sbjct: 228 LSTCPWKEELEVLIRGGVPMALRGELWQAFVGVRKRRC---KDYYQNLL-AADGSVNTIE 287

Query: 157 R------------------------QIDHDLPRTFPGHPWLDTPAGHAALRRVLVGYSFR 216
           +                        QI+ DLPRTFPGHP LD   G  ALRR+L  Y+  
Sbjct: 288 QEDMQHVDDKGSSTESIAVVEKWKGQIEKDLPRTFPGHPALDDD-GRNALRRLLTAYARH 347

Query: 217 DSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLGFNSVSHHGLSMPNSSLSFY 276
           +  VGYCQ                                                    
Sbjct: 348 NPSVGYCQ---------------------------------------------------- 407

Query: 277 PRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVTRPTCQDVMSSNGYSKIYLP 336
                   +N+ AALLLL+M  EE+AFW L  L+++               NGY    + 
Sbjct: 408 -------AMNFFAALLLLLM-PEENAFWALIGLIDDYF-------------NGYYSEEMI 467

Query: 337 KSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFDVSLVATEWFLCLFSKSLPS 396
           +S        D  +L    +  +      ++  HL+ L   V+ V   WFL +F   LP 
Sbjct: 468 ESQ------VDQLVLEELVRERFP-----KLVHHLDYLGVQVAWVTGPWFLSIFMNMLPW 527

Query: 397 ETTLRVWDVLFYEGAKV-LFHVALAIFKMKEGELLISHHVGDVINILQKTTHNLFDPDDL 456
           E+ LRVWDVL +EG +V LF  ALA+ ++    L+ +   GD + +LQ  T + FD   L
Sbjct: 528 ESVLRVWDVLLFEGTRVMLFRTALALMELYGPALVTTKDAGDAVTLLQSLTGSTFDSSQL 538

Query: 457 LTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKKQEPAVMAELDQRLRRLNS 491
                           ++   + Y  +  +    + + R K  PAV+A L++R + L +
Sbjct: 588 ----------------VLTACMGYQNVHEI---RLQELRSKHRPAVIAALEERSKGLQA 538

BLAST of Lsi10G000660 vs. TAIR 10
Match: AT2G39280.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-24
Identity = 113/438 (25.80%), Postives = 175/438 (39.95%), Query Frame = 0

Query: 81  LEPSVSEGIALKSSLKLSTLANAIT-------LKKLIRKGIPPVLRPKVWFSLSGAAKKK 140
           ++   S+G ++ S    +  A+ ++       L+ LI  G P  LR ++W + +G  K++
Sbjct: 192 IQDGSSDGTSVSSMSAAADAASLVSACPWKDELEVLIHGGAPMALRGELWQAFAGVKKRR 251

Query: 141 -------STVPDSYYNDLTKAV------EGKVTPATR-------QIDHDLPRTFPGHPWL 200
                      DS  ND+ + +      +G  T           QI+ DLPRTFPGHP L
Sbjct: 252 VKNYYQNLLAADSLGNDIEQELMQHTDEKGSSTDPLSVVEKWKGQIEKDLPRTFPGHPAL 311

Query: 201 DTPAGHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLG 260
           D    + ALRR+L  Y+  +  VGYCQ                                 
Sbjct: 312 DDDFRN-ALRRLLTAYARHNPSVGYCQ--------------------------------- 371

Query: 261 FNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVT 320
                                      +N+ AALLLL+M  EE+AFW L  ++       
Sbjct: 372 --------------------------AMNFFAALLLLLM-PEENAFWSLTGII------- 431

Query: 321 RPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFD 380
                D    + YS+  L   V       D  +L    +  +      ++  HL+ L   
Sbjct: 432 -----DDYFHDYYSEEMLESQV-------DQRVLEELLRERFP-----KLVHHLDYLGVQ 491

Query: 381 VSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKV-LFHVALAIFKMKEGELLISHHVG 440
           V+ V   WFL +F   LP E+ LRVWDVL +EG +V LF  ALA+ +     L+ +  +G
Sbjct: 492 VACVTGPWFLTIFINMLPWESVLRVWDVLLFEGNRVMLFRTALALMEFYGPALVTTKDIG 525

Query: 441 DVINILQKTTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKK 491
           D + +LQ  T + FD   L+ +       + ES                    + + R K
Sbjct: 552 DAVTLLQSMTGSTFDSSQLVFTACMGYQNVHES-------------------KLQELRSK 525

BLAST of Lsi10G000660 vs. TAIR 10
Match: AT2G39280.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-24
Identity = 113/438 (25.80%), Postives = 175/438 (39.95%), Query Frame = 0

Query: 81  LEPSVSEGIALKSSLKLSTLANAIT-------LKKLIRKGIPPVLRPKVWFSLSGAAKKK 140
           ++   S+G ++ S    +  A+ ++       L+ LI  G P  LR ++W + +G  K++
Sbjct: 220 IQDGSSDGTSVSSMSAAADAASLVSACPWKDELEVLIHGGAPMALRGELWQAFAGVKKRR 279

Query: 141 -------STVPDSYYNDLTKAV------EGKVTPATR-------QIDHDLPRTFPGHPWL 200
                      DS  ND+ + +      +G  T           QI+ DLPRTFPGHP L
Sbjct: 280 VKNYYQNLLAADSLGNDIEQELMQHTDEKGSSTDPLSVVEKWKGQIEKDLPRTFPGHPAL 339

Query: 201 DTPAGHAALRRVLVGYSFRDSDVGYCQHNVRTGFNPYTLLVVCVICIRYSTSFLLSLKLG 260
           D    + ALRR+L  Y+  +  VGYCQ                                 
Sbjct: 340 DDDFRN-ALRRLLTAYARHNPSVGYCQ--------------------------------- 399

Query: 261 FNSVSHHGLSMPNSSLSFYPRPPHPVGLNYVAALLLLVMKTEEDAFWMLAVLLENSVTVT 320
                                      +N+ AALLLL+M  EE+AFW L  ++       
Sbjct: 400 --------------------------AMNFFAALLLLLM-PEENAFWSLTGII------- 459

Query: 321 RPTCQDVMSSNGYSKIYLPKSVPGASLCFDIHLLSNKYKHFYKLECKCRIAAHLEALDFD 380
                D    + YS+  L   V       D  +L    +  +      ++  HL+ L   
Sbjct: 460 -----DDYFHDYYSEEMLESQV-------DQRVLEELLRERFP-----KLVHHLDYLGVQ 519

Query: 381 VSLVATEWFLCLFSKSLPSETTLRVWDVLFYEGAKV-LFHVALAIFKMKEGELLISHHVG 440
           V+ V   WFL +F   LP E+ LRVWDVL +EG +V LF  ALA+ +     L+ +  +G
Sbjct: 520 VACVTGPWFLTIFINMLPWESVLRVWDVLLFEGNRVMLFRTALALMEFYGPALVTTKDIG 553

Query: 441 DVINILQKTTHNLFDPDDLLTSHFFSDDGLKESFWIVNLQVAYDKIGSMTTNTISKQRKK 491
           D + +LQ  T + FD   L+ +       + ES                    + + R K
Sbjct: 580 DAVTLLQSMTGSTFDSSQLVFTACMGYQNVHES-------------------KLQELRSK 553

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6ZT071.2e-2527.18TBC1 domain family member 9 OS=Homo sapiens OX=9606 GN=TBC1D9 PE=2 SV=2[more]
Q9BYX22.0e-2529.24TBC1 domain family member 2A OS=Homo sapiens OX=9606 GN=TBC1D2 PE=1 SV=3[more]
B1AVH72.6e-2529.24TBC1 domain family member 2A OS=Mus musculus OX=10090 GN=Tbc1d2 PE=2 SV=1[more]
Q3UYK37.5e-2527.32TBC1 domain family member 9 OS=Mus musculus OX=10090 GN=Tbc1d9 PE=2 SV=2[more]
A6QP292.2e-2429.59TBC1 domain family member 2A OS=Bos taurus OX=9913 GN=TBC1D2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UC133.6e-18773.99TBC1 domain family member 2A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0LQ821.8e-18673.99Rab-GAP TBC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G406820... [more]
A0A1S4E0V72.0e-18572.78small G protein signaling modulator 3 OS=Cucumis melo OX=3656 GN=LOC103496045 PE... [more]
A0A0H4TH701.3e-18473.19TBC1 domain family member 2A OS=Momordica charantia OX=3673 GN=LOC111010987 PE=2... [more]
A0A6J1G6E22.2e-18172.38growth hormone-regulated TBC protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
KAA0053282.17.3e-18773.99TBC1 domain family member 2A [Cucumis melo var. makuwa] >TYK11875.1 TBC1 domain ... [more]
XP_038900967.11.3e-18673.99growth hormone-regulated TBC protein 1 [Benincasa hispida][more]
XP_004138606.13.6e-18673.99growth hormone-regulated TBC protein 1 [Cucumis sativus] >XP_011649971.1 growth ... [more]
XP_016901858.14.0e-18572.78PREDICTED: small G protein signaling modulator 3 [Cucumis melo][more]
XP_022140274.12.6e-18473.19growth hormone-regulated TBC protein 1 [Momordica charantia] >AKQ06194.1 TBC1 do... [more]
Match NameE-valueIdentityDescription
AT3G07890.14.5e-17467.13Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT3G07890.24.5e-17467.13Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT3G55020.11.2e-2526.97Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT2G39280.12.2e-2425.80Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT2G39280.22.2e-2425.80Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000195Rab-GTPase-TBC domainSMARTSM00164tbc_4coord: 110..409
e-value: 9.5E-33
score: 124.8
IPR000195Rab-GTPase-TBC domainPFAMPF00566RabGAP-TBCcoord: 118..200
e-value: 1.7E-11
score: 44.2
coord: 260..406
e-value: 1.4E-20
score: 73.8
IPR000195Rab-GTPase-TBC domainPROSITEPS50086TBC_RABGAPcoord: 113..386
score: 22.372328
NoneNo IPR availableGENE3D1.10.8.270putative rabgap domain of human tbc1 domain family member 14 like domainscoord: 129..292
e-value: 1.3E-25
score: 91.6
NoneNo IPR availableGENE3D1.10.472.80coord: 326..443
e-value: 2.9E-27
score: 97.3
NoneNo IPR availableGENE3D1.10.10.750coord: 99..128
e-value: 5.4E-6
score: 28.4
NoneNo IPR availablePANTHERPTHR22957:SF589RAB-GTPASE-TBC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 333..448
coord: 1..200
NoneNo IPR availablePANTHERPTHR22957:SF589RAB-GTPASE-TBC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 260..292
NoneNo IPR availablePANTHERPTHR22957TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEINcoord: 333..448
coord: 1..200
NoneNo IPR availablePANTHERPTHR22957TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEINcoord: 260..292
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 105..200
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 260..290
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 343..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G000660.1Lsi10G000660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090630 activation of GTPase activity
biological_process GO:0006886 intracellular protein transport
molecular_function GO:0005096 GTPase activator activity