Lsi10G000120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G000120
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description3-hydroxy-3-methylglutaryl coenzyme A reductase
Locationchr10: 252497 .. 256657 (+)
RNA-Seq ExpressionLsi10G000120
SyntenyLsi10G000120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGTATCTTCTTAAATTTGGTATTTAGTTACAATAAATTTATGTTTCCTATGCTTAAACCCAACCCGTGTAATTTTTTTTCTTAATAATTTAGACCTCGTTTGGTAACTATTTTGTTTTTAGTTTTTTTATTTTTGAAAATTAAGTCTATTTCCTCCCACTTTCTTAACTGGTTTGCATCTTTCTTAAATATAAGAATTACATTCTTAACCAAATTCTAAAAACAAAAACAAATTTTTAAAAATTACTTTTTTTAGTTTTTAAAATTTGATTTGGTTTTTGAAAACATAGGTAAAAGTAGATAAGAAAACAAAAAATTTAAAAGTGAGAAAGGTGATTATAAGCTTAATTTTCAAAAATCAATTAGTTACCAAACGAGACCTTAATCTTTATAATTTAAAACATAAAGCTATTAAGTCAAACAATACTTCGATAGTAATGTTCATTTTTTCACAATTAAATTTTTTAAATGTATTTATCTAGAAAAAGAAAAAAACTTATAGTATAAACTTTTTAGTACAATAAATAGAGGAAATTCAAACTACATATATCTTAATCGCTAACATATATTCTATGTCGGTTAAGTTAAACTCACTTATATCACTATACAAAATTTAATAGACATGTTGTCAATTAATAAAGCAAATATTGTTTGGTTTGGCTAAGTCATAGTCATTAAAAAATGCGGCATTTTTTTTTTTTTTTAAAATAAAGCTATAGAGGTAAACGTGTATTTAAAGTAATTTATTTTTATAAAATTTAGAAAATTACACTTTCATTGTCTAAATTCTAAATTTAATTTTAGTATGATCTCCATACACCATGTAAAATACTCCAAATTTAAGGAACCAAAACCATAATTTACCTTAAATAAAAAAAATTAAATAATTAAATCTAATTTATGTAGATTTCTTAAACAATTAATGCCCTATTTGATAATCGTTTATAGTTTTTTAACATTGTTTTTGTTTCTTAAAATTCTTTTACCATAGTTTTCACATTTTCGAACGAAAGATATGAATTTAGTCAAATTAGAAAAAAAAAACCTAATTTATCTACTACTTTTAATTTAATAAACTTTACTTGTATTTTGATAACATTTGTGAAAAGAAAATAATAAAACAAACATAGTGTTTCATACTCTTTTCAAATATAGAAAAATGAACCAAACTATTTATAAATATAGAAATTTTTTACAGCCTATCAGTGATAGACCGCTATAGAATTCTATCGCTTTTTATTGCTCAAGCGATATAATTCATTTGCTTGAGCGATAGAATGCTTTCTATCGCGGTTTATCACTGATAGATAGTGAAATTTTTCTATATTTGTAAATAGTTTGATATTTTTTCTGTTTATAGTAATTTTTCTAGTGTTAATGTGTTTTTTTTTATTTTTAAAATCAACTAGTTATCAAAGTGCAATTAACTCTAATATTATAAAATAAAGGGGACTTTTTAAAAATAGAAAAATAAATGAAACTATTTACACAAAATAGCAAAAATTTTAGATAGTTTGATAGATGATGATAGAAGTCTATCAGGGTCTATCGGTGATAGAAATGATATAAATCTATTACTGGTAGATGCTGATAGAAGTCTATCAATATCTATCCGTGATTTTTTTGCTATTTTCTGTAAATAGTTTGATATTTTTTTTTATTTGTGAAAATTTCTCTAAAATAAATTTTCGAAAAATGATTTAAAACTATTTTATTAAAAAAAAAAAGTCCAAACCTCGTGGCTCAAAACCCAACTTACCATTTAAAAAGCATTATTAATTATTAAATAATGAAATAGTGTTAGAATTAATTAATCCACAAAAATAAAATATGATATTAGTTTCCATATTTACATGATTGCTCATTGCTAACTTACAACCCACAACCACAGAGAAGAATAATGAACCCATCCCGCTCGGTCCGGTCCGTCTGAGCTGGCCAGGTTCGGTGGGACCCACACCCCCCTTTCCACCAAAAGATTTAAAAATAAAAAATAAAACCCCTATAAATACGCCCAAATCCTCGCTTCACCCTTCAAAATTTTCTAAACCATTTCCCCCATTTGAAAGGAGGAAAGAAAAGGGCGAAAGAAAAGGAAAAATCTTCATTTCATTATAAAACTCCCTCCGCCTCCCGATCCGGCGCCGGCGTTTTCCATTTTCACTTTTCTCTTGCCCAACAATGGACGCCCGCCGCCGCCGGTCCTCTTCTCTCGTCAAGAAACTCACCGCCGACGAACCACCGGTTGAATCCATGGTTAAAAATTCTCTCAAGGTTAAGATGGTGGAACGGCAACAACATGTTCATGATGATGCCATTAAGGCGTCTGATGTTTTGCCTCTTCCCCTTTACCTCACCAATGCCGCTTTCTTCACTCTCTTCTTCTCTGTTGTTTACTTTCTTCTTACGCGGTGGCGTGAGAAGATTCGTTCATCCACTCCTCTCCATGTCGTTACGCTTTCCGAAATGGTCGCCATTTCTGCGTTTATCGCTTCCTTCATTTACCTTCTCGGATTCTTCGGAATCGACTTTGTTCAGTCGATTTTCCGTCCTTCGCATGATGTATGGACCTCTGAGGATGATGAGGTCGTTTTGATTAAGGAGGATACTCGGAAGGTTCCGTGTGGTGCTGGAATCGATTGTTCGATTCCGATTTTGGCACCTCCTTTGCAATCAGTGCCAAAAGTGGTTGATCCGGTACCTGTTAGCATCGATTTGACGGAGGAGGATGAAGAAATCGTTAAATCTGTTGTGGCTGGAACTACGCCGTCGTATTCGCTCGAATCGAAGCTTGGCGATTGTGGACGAGCGGCGGCTATCCGTCGCGAGGCCTTGCAGAGAGTTACCGGGAAATCGCTCTCGGGGCTTCCTTTGGAAGGTTTCGATTACGCTTCGATATTGGGGCAGTGCTGCGAGATGCCTATTGGATATGTACAGATTCCGGTGGGAATCGCTGGGCCATTGTTGTTAGACGGCAAGGAGTATTCTGTACCAATGGCCACTACCGAAGGTTGCTTGGTTGCTAGTACAAATAGAGGTTGTAAAGCGATTATGATGTCCGGTGGAGCTAACAGCGTATTGTTGAGAGATGCAATGACGAGAGCACCGGTTATGAGATTCGCTACGGCGAAAAGAGCAGCGGAATTGAAGATGTACGTTGAGGATCCGGTGAATTTCGATACCTTAGCCTCCGTTTTTAACAAGTCCAGTAGATTTGGAAGACTACAAAGTATCAAATGCGCCATTGCTGGTAAGAATCTCTATATGAGATTCTCTTGCAGTACTGGTGATGCTATGGGGATGAATATGGTGTCCAAAGGCGTTCAAAACGTTTTGGATTTTCTCCAGAGTGATTTCCCTGACATGGATGTAATTGGCATATCTGGTAACAGAGCAAAATTCCTTCCACACAACTCTTCCAATTTTCTCTGATTTACTTCTTTTAAACTGTATTCGTTTCTTAATCCAGGAAACTACTGTTCGGATAAGAAGCCAGCTGCTGTGAACTGGATCGAAGGGCGTGGGAAATCGGTGGTTTGTGAGGTTATAATCAAGGGTGACGTAGTTAAGAAGGTCCTCAAAACCGACGTTCAAGCCTTAGTGGAGCTTAACATGCTGAAGAATCTCACTGGTTCTGCCATGGCTGGAGCTCTTGGAGGGTTCAACGCCCATGCCAGTAACATCGTTTCAGCCATCTTTATCGCAACAGGGCAAGATCCTGCACAGAACGTGGAGAGTTCTCACTGTATTACAATGATGGAAGCTGTGAATGATGGCCAAGATCTTCACGTCTCTGTTACCATGCCTTCCATAGAGGTGGGTGAATTTACTTTTCATAATAATCTTCTTTATGATCTAATTGTTGAAATTTCTGAGTCTAAGCAGTTGGTTTAATTCATTGTTTAATGCAGGTGGGTACAGTTGGAGGAGGGACCCAGCTTGCTTCTCAATCTGCTTGTTTGAACTTACTTGGAGTTAAAGGGGCCAACAGAGAAGCCCCAGGATCTAACTCAAGGCTGCTAGCCAGCATTGTAGCTGGGTCAGTTCTTGCAGGAGAGCTTTCTCTTATGTCTGCAATTTCAGCAGGACATCTTGTGAGGAGTCATATGAAGTACAATAGATCGAGTAGAGATGTCACTAAGGCTTCTTCCTCTTAA

mRNA sequence

ATGCATGAGGAAAGAAAAGGGCGAAAGAAAAGGAAAAATCTTCATTTCATTATAAAACTCCCTCCGCCTCCCGATCCGGCGCCGGCGTTTTCCATTTTCACTTTTCTCTTGCCCAACAATGGACGCCCGCCGCCGCCGAAACTCACCGCCGACGAACCACCGGTTGAATCCATGGTTAAAAATTCTCTCAAGGTTAAGATGGTGGAACGGCAACAACATGTTCATGATGATGCCATTAAGGCGTCTGATGTTTTGCCTCTTCCCCTTTACCTCACCAATGCCGCTTTCTTCACTCTCTTCTTCTCTGTTGTTTACTTTCTTCTTACGCGGTGGCGTGAGAAGATTCGTTCATCCACTCCTCTCCATGTCGTTACGCTTTCCGAAATGGTCGCCATTTCTGCGTTTATCGCTTCCTTCATTTACCTTCTCGGATTCTTCGGAATCGACTTTGTTCAGTCGATTTTCCGTCCTTCGCATGATGTATGGACCTCTGAGGATGATGAGGTCGTTTTGATTAAGGAGGATACTCGGAAGGTTCCGTGTGGTGCTGGAATCGATTGTTCGATTCCGATTTTGGCACCTCCTTTGCAATCAGTGCCAAAAGTGGTTGATCCGGTACCTGTTAGCATCGATTTGACGGAGGAGGATGAAGAAATCGTTAAATCTGTTGTGGCTGGAACTACGCCGTCGTATTCGCTCGAATCGAAGCTTGGCGATTGTGGACGAGCGGCGGCTATCCGTCGCGAGGCCTTGCAGAGAGTTACCGGGAAATCGCTCTCGGGGCTTCCTTTGGAAGGTTTCGATTACGCTTCGATATTGGGGCAGTGCTGCGAGATGCCTATTGGATATGTACAGATTCCGGTGGGAATCGCTGGGCCATTGTTGTTAGACGGCAAGGAGTATTCTGTACCAATGGCCACTACCGAAGGTTGCTTGGTTGCTAGTACAAATAGAGGTTGTAAAGCGATTATGATGTCCGGTGGAGCTAACAGCGTATTGTTGAGAGATGCAATGACGAGAGCACCGGTTATGAGATTCGCTACGGCGAAAAGAGCAGCGGAATTGAAGATGTACGTTGAGGATCCGGTGAATTTCGATACCTTAGCCTCCGTTTTTAACAAGTCCAGTAGATTTGGAAGACTACAAAGTATCAAATGCGCCATTGCTGGTAAGAATCTCTATATGAGATTCTCTTGCAGTACTGGTGATGCTATGGGGATGAATATGGTGTCCAAAGGCGTTCAAAACGTTTTGGATTTTCTCCAGAGTGATTTCCCTGACATGGATGTAATTGGCATATCTGGAAACTACTGTTCGGATAAGAAGCCAGCTGCTGTGAACTGGATCGAAGGGCGTGGGAAATCGGTGGTTTGTGAGGTTATAATCAAGGGTGACGTAGTTAAGAAGGTCCTCAAAACCGACGTTCAAGCCTTAGTGGAGCTTAACATGCTGAAGAATCTCACTGGTTCTGCCATGGCTGGAGCTCTTGGAGGGTTCAACGCCCATGCCAGTAACATCGTTTCAGCCATCTTTATCGCAACAGGGCAAGATCCTGCACAGAACGTGGAGAGTTCTCACTGTATTACAATGATGGAAGCTGTGAATGATGGCCAAGATCTTCACGTCTCTGTTACCATGCCTTCCATAGAGGTGGGTGAATTTACTTTTCATAATAATCTTCTTTATGATCTAATTGTTGAAATTTCTGAGTCTAAGCAGTTGGTGGGTACAGTTGGAGGAGGGACCCAGCTTGCTTCTCAATCTGCTTGTTTGAACTTACTTGGAGTTAAAGGGGCCAACAGAGAAGCCCCAGGATCTAACTCAAGGCTGCTAGCCAGCATTGTAGCTGGGTCAGTTCTTGCAGGAGAGCTTTCTCTTATGTCTGCAATTTCAGCAGGACATCTTGTGAGGAGTCATATGAAGTACAATAGATCGAGTAGAGATGTCACTAAGGCTTCTTCCTCTTAA

Coding sequence (CDS)

ATGCATGAGGAAAGAAAAGGGCGAAAGAAAAGGAAAAATCTTCATTTCATTATAAAACTCCCTCCGCCTCCCGATCCGGCGCCGGCGTTTTCCATTTTCACTTTTCTCTTGCCCAACAATGGACGCCCGCCGCCGCCGAAACTCACCGCCGACGAACCACCGGTTGAATCCATGGTTAAAAATTCTCTCAAGGTTAAGATGGTGGAACGGCAACAACATGTTCATGATGATGCCATTAAGGCGTCTGATGTTTTGCCTCTTCCCCTTTACCTCACCAATGCCGCTTTCTTCACTCTCTTCTTCTCTGTTGTTTACTTTCTTCTTACGCGGTGGCGTGAGAAGATTCGTTCATCCACTCCTCTCCATGTCGTTACGCTTTCCGAAATGGTCGCCATTTCTGCGTTTATCGCTTCCTTCATTTACCTTCTCGGATTCTTCGGAATCGACTTTGTTCAGTCGATTTTCCGTCCTTCGCATGATGTATGGACCTCTGAGGATGATGAGGTCGTTTTGATTAAGGAGGATACTCGGAAGGTTCCGTGTGGTGCTGGAATCGATTGTTCGATTCCGATTTTGGCACCTCCTTTGCAATCAGTGCCAAAAGTGGTTGATCCGGTACCTGTTAGCATCGATTTGACGGAGGAGGATGAAGAAATCGTTAAATCTGTTGTGGCTGGAACTACGCCGTCGTATTCGCTCGAATCGAAGCTTGGCGATTGTGGACGAGCGGCGGCTATCCGTCGCGAGGCCTTGCAGAGAGTTACCGGGAAATCGCTCTCGGGGCTTCCTTTGGAAGGTTTCGATTACGCTTCGATATTGGGGCAGTGCTGCGAGATGCCTATTGGATATGTACAGATTCCGGTGGGAATCGCTGGGCCATTGTTGTTAGACGGCAAGGAGTATTCTGTACCAATGGCCACTACCGAAGGTTGCTTGGTTGCTAGTACAAATAGAGGTTGTAAAGCGATTATGATGTCCGGTGGAGCTAACAGCGTATTGTTGAGAGATGCAATGACGAGAGCACCGGTTATGAGATTCGCTACGGCGAAAAGAGCAGCGGAATTGAAGATGTACGTTGAGGATCCGGTGAATTTCGATACCTTAGCCTCCGTTTTTAACAAGTCCAGTAGATTTGGAAGACTACAAAGTATCAAATGCGCCATTGCTGGTAAGAATCTCTATATGAGATTCTCTTGCAGTACTGGTGATGCTATGGGGATGAATATGGTGTCCAAAGGCGTTCAAAACGTTTTGGATTTTCTCCAGAGTGATTTCCCTGACATGGATGTAATTGGCATATCTGGAAACTACTGTTCGGATAAGAAGCCAGCTGCTGTGAACTGGATCGAAGGGCGTGGGAAATCGGTGGTTTGTGAGGTTATAATCAAGGGTGACGTAGTTAAGAAGGTCCTCAAAACCGACGTTCAAGCCTTAGTGGAGCTTAACATGCTGAAGAATCTCACTGGTTCTGCCATGGCTGGAGCTCTTGGAGGGTTCAACGCCCATGCCAGTAACATCGTTTCAGCCATCTTTATCGCAACAGGGCAAGATCCTGCACAGAACGTGGAGAGTTCTCACTGTATTACAATGATGGAAGCTGTGAATGATGGCCAAGATCTTCACGTCTCTGTTACCATGCCTTCCATAGAGGTGGGTGAATTTACTTTTCATAATAATCTTCTTTATGATCTAATTGTTGAAATTTCTGAGTCTAAGCAGTTGGTGGGTACAGTTGGAGGAGGGACCCAGCTTGCTTCTCAATCTGCTTGTTTGAACTTACTTGGAGTTAAAGGGGCCAACAGAGAAGCCCCAGGATCTAACTCAAGGCTGCTAGCCAGCATTGTAGCTGGGTCAGTTCTTGCAGGAGAGCTTTCTCTTATGTCTGCAATTTCAGCAGGACATCTTGTGAGGAGTCATATGAAGTACAATAGATCGAGTAGAGATGTCACTAAGGCTTCTTCCTCTTAA

Protein sequence

MHEERKGRKKRKNLHFIIKLPPPPDPAPAFSIFTFLLPNNGRPPPPKLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSSRDVTKASSS
Homology
BLAST of Lsi10G000120 vs. ExPASy Swiss-Prot
Match: O64966 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 OS=Gossypium hirsutum OX=3635 GN=HMG1 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 6.9e-252
Identity = 462/601 (76.87%), Postives = 512/601 (85.19%), Query Frame = 0

Query: 61  NSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYFLLTRWREKIRSSTP 120
           NS++     R   + DD+ KASD LPLPLYLTNA FFTLFFS VYFLL RWREKIRSSTP
Sbjct: 10  NSIRSHKPARPIALEDDSTKASDALPLPLYLTNAVFFTLFFSAVYFLLCRWREKIRSSTP 69

Query: 121 LHVVTLSEMVAISAFIASFIYLLGFFGIDFVQS-IFRPSHDVWTSEDD----EVVLIKED 180
           LHVVT SE+VAI A +ASFIYLLGFFGIDFVQS + RPS DVW +EDD    EV+L  ED
Sbjct: 70  LHVVTFSEIVAILASVASFIYLLGFFGIDFVQSLVLRPSADVWATEDDEVESEVLLRNED 129

Query: 181 TRKVPCGAGIDCSIPILAP--PLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAGTTPSYSL 240
            R VPCG  +D SI  L P  P+ +  KV D +PV++ +TEEDEEI++SVV G TPSYSL
Sbjct: 130 ARHVPCGQALDRSIRSLQPPEPIVTAEKVFDEMPVTV-MTEEDEEIIRSVVCGMTPSYSL 189

Query: 241 ESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQIPVGIAGP 300
           ESKL DC RAAAIRREALQR+TGKSLSGLPL+GFDY SILGQCCEMP+GY QIPVGIAGP
Sbjct: 190 ESKLDDCKRAAAIRREALQRITGKSLSGLPLDGFDYESILGQCCEMPVGYEQIPVGIAGP 249

Query: 301 LLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRFATAKRAA 360
           LLL+G+EYSVPMATTEGCLVASTNRGCKAI +SGGA SVLLRD MTRAPV+RF TAKRAA
Sbjct: 250 LLLNGREYSVPMATTEGCLVASTNRGCKAIHLSGGATSVLLRDGMTRAPVVRFGTAKRAA 309

Query: 361 ELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMGMNMVSKG 420
           +LK+Y+EDP NF+TLA VFN+SSRF RLQSIKCAIAGKNLY+RFSC TGDAMGMNMVSKG
Sbjct: 310 DLKLYLEDPENFETLACVFNRSSRFARLQSIKCAIAGKNLYLRFSCFTGDAMGMNMVSKG 369

Query: 421 VQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDVVKKVLKT 480
           VQNVLDFLQ+DFPDMDVIGISGN+CSDKKPAAVNWIEGRGKSVVCE II GDVV KVLKT
Sbjct: 370 VQNVLDFLQTDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAIINGDVVTKVLKT 429

Query: 481 DVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEA 540
            V++LVELNMLKNLTGSAMAGALGGFNAHASNIV+A++IATGQDPAQNVESSHCITMMEA
Sbjct: 430 SVESLVELNMLKNLTGSAMAGALGGFNAHASNIVTAVYIATGQDPAQNVESSHCITMMEA 489

Query: 541 VNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLASQSACLNL 600
           VN G+DLHVSVTMPSIE                        VGTVGGGTQLASQSACLNL
Sbjct: 490 VNGGKDLHVSVTMPSIE------------------------VGTVGGGTQLASQSACLNL 549

Query: 601 LGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSSRDVTKAS 655
           LGVKGA++E+PG+NS LLA+IVAG+VLAGELSLMSA++AG LV+SHMKYNRSS+DV+K S
Sbjct: 550 LGVKGASKESPGANSILLATIVAGAVLAGELSLMSALAAGQLVKSHMKYNRSSKDVSKVS 585

BLAST of Lsi10G000120 vs. ExPASy Swiss-Prot
Match: P48021 (3-hydroxy-3-methylglutaryl-coenzyme A reductase OS=Camptotheca acuminata OX=16922 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 4.6e-248
Identity = 462/624 (74.04%), Postives = 519/624 (83.17%), Query Frame = 0

Query: 37  LPNNGRPPPPKLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAF 96
           +P+ G   PP L   +P          KV  V+       D+ KASD LPLPLY+TN  F
Sbjct: 14  IPSVGGTAPPMLKPKQP---------TKVDAVDL-----PDSPKASDALPLPLYITNGVF 73

Query: 97  FTLFFSVVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSI-F 156
           FTLFF+VVY+LL RWREKIR+STPLHVVTLSE+ AI  F+ASFIYLLGFFGI  VQ    
Sbjct: 74  FTLFFTVVYYLLVRWREKIRNSTPLHVVTLSEIAAIFTFVASFIYLLGFFGIGLVQPFTS 133

Query: 157 RPSH-DVWTSEDDE---VVLIKEDTRKVPCGAG-IDCSIPILAPPLQSVPKVVDPVPVSI 216
           R SH DVW  +DDE    +++KEDTR VPC A  +DC +    PP++  PKVVDPVP+S 
Sbjct: 134 RSSHDDVWGVDDDEDVDEIVLKEDTRTVPCAAAPVDCPL----PPIK--PKVVDPVPISP 193

Query: 217 DLTEEDEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYA 276
             +EEDEEI+KSVV GTTPSY+LESKLGD  RAAAIRREALQR+T KSL+GLPL+GFDY 
Sbjct: 194 PSSEEDEEIIKSVVEGTTPSYALESKLGDSHRAAAIRREALQRMTKKSLAGLPLDGFDYD 253

Query: 277 SILGQCCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGAN 336
           SILGQCCEMP+GYVQIPVGIAGPLLLDG+EYSVPMATTEGCLVASTNRGCKAI   GGA 
Sbjct: 254 SILGQCCEMPVGYVQIPVGIAGPLLLDGREYSVPMATTEGCLVASTNRGCKAIFACGGAT 313

Query: 337 SVLLRDAMTRAPVMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAG 396
           SVLLRDAMTRAPV+RF +AKRAA+LK ++E+P+NF+TLA+VFN SSRFG+LQ+IKCAIAG
Sbjct: 314 SVLLRDAMTRAPVVRFGSAKRAADLKFFLENPLNFETLAAVFNSSSRFGKLQNIKCAIAG 373

Query: 397 KNLYMRFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIE 456
           KNLYMR+SCSTGDAMGMNM+SKGVQNVLDFLQ DFPDMDVIGISGNYCSDKKPAAVNWIE
Sbjct: 374 KNLYMRYSCSTGDAMGMNMISKGVQNVLDFLQDDFPDMDVIGISGNYCSDKKPAAVNWIE 433

Query: 457 GRGKSVVCEVIIKGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAI 516
           GRGKSVVCE +IK +VVKKVLKT+V +LVELNMLKNLTGSAMAGALGGFNAHASNIVSA+
Sbjct: 434 GRGKSVVCEAVIKEEVVKKVLKTNVASLVELNMLKNLTGSAMAGALGGFNAHASNIVSAV 493

Query: 517 FIATGQDPAQNVESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISE 576
           ++ATGQDPAQNVESSHCITMMEA+NDG+DLHVSVTMPSIE                    
Sbjct: 494 YLATGQDPAQNVESSHCITMMEAINDGKDLHVSVTMPSIE-------------------- 553

Query: 577 SKQLVGTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAI 636
               VGTVGGGTQLASQSACLNLLGVKGA++EAPGSN+RLLA+IVAGSVLAGELSLMSAI
Sbjct: 554 ----VGTVGGGTQLASQSACLNLLGVKGASKEAPGSNARLLATIVAGSVLAGELSLMSAI 593

Query: 637 SAGHLVRSHMKYNRSSRDVTKASS 655
           +AG LV SHMKYNRS++DVTKASS
Sbjct: 614 AAGQLVNSHMKYNRSNKDVTKASS 593

BLAST of Lsi10G000120 vs. ExPASy Swiss-Prot
Match: O64967 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 2 OS=Gossypium hirsutum OX=3635 GN=HMG2 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.3e-245
Identity = 459/646 (71.05%), Postives = 516/646 (79.88%), Query Frame = 0

Query: 57  SMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYFLLTRWREKIR 116
           S VK  + +K  +R   V D   KASD LPLPLYLTNA FFTLFF+VVYFLL+RWREKIR
Sbjct: 7   SAVKPVIVLKPSKRFPLVEDTPGKASDALPLPLYLTNAVFFTLFFTVVYFLLSRWREKIR 66

Query: 117 SSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQS-IFRPSHDVWTSED----DEVVL 176
           +S PLH VT  E+VAI AF+AS IYLLGFFGIDFVQS I RPS DVW+ ED    +EV+L
Sbjct: 67  ASIPLHAVTFPEIVAIFAFVASLIYLLGFFGIDFVQSLIIRPSGDVWSGEDYEEENEVLL 126

Query: 177 IKEDTRKVPCGAGIDCSIPILAPPLQSV--PKVVDPVPVS-------------------- 236
            +ED R VPCG  +DCS+P L    ++V   ++ D  PV                     
Sbjct: 127 HEEDARTVPCGQALDCSVPSLPHMARNVTAQRLFDEKPVRVATEEDARKVSCGQAVDCSL 186

Query: 237 ---------------------IDLTEEDEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRR 296
                                I  TEEDEEI+KSVVAGT PSYSLESKLGDC RAAAIRR
Sbjct: 187 HSLPPRPPIVTSQKLFHEKTVIVTTEEDEEIIKSVVAGTLPSYSLESKLGDCKRAAAIRR 246

Query: 297 EALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATT 356
           EALQR+TG+SLSGLPL+GFDY SILGQCCEMP+GYVQIPVGIAGPLLL+G+EYSVPMATT
Sbjct: 247 EALQRLTGRSLSGLPLDGFDYESILGQCCEMPVGYVQIPVGIAGPLLLNGREYSVPMATT 306

Query: 357 EGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRFATAKRAAELKMYVEDPVNFDTL 416
           EGCLVASTNRGCKAI +SGGA S+LL+D MTRAPV+RF+TAKRAAELK Y+EDP NFDTL
Sbjct: 307 EGCLVASTNRGCKAIHLSGGATSILLKDGMTRAPVVRFSTAKRAAELKFYLEDPENFDTL 366

Query: 417 ASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDM 476
           A VFN+SSRFGRLQSIKCAIAGKNLY+RF+CSTGDAMGMNMVSKGVQNVLDFLQ+DFPDM
Sbjct: 367 AVVFNRSSRFGRLQSIKCAIAGKNLYLRFTCSTGDAMGMNMVSKGVQNVLDFLQTDFPDM 426

Query: 477 DVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDVVKKVLKTDVQALVELNMLKNLT 536
           DVIGISGN+CSDKKPAAVNWIEGRGKSVVCE II+GDVV+KVLKT V++LVELNMLKNLT
Sbjct: 427 DVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAIIEGDVVRKVLKTSVESLVELNMLKNLT 486

Query: 537 GSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEAVNDGQDLHVSVTMPS 596
           GSAMAGALGGFNAHASNIV+AI+IATGQDPAQNVESSHCITMMEAVNDG+DLH+SVTMPS
Sbjct: 487 GSAMAGALGGFNAHASNIVTAIYIATGQDPAQNVESSHCITMMEAVNDGKDLHISVTMPS 546

Query: 597 IEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLASQSACLNLLGVKGANREAPGSNS 655
           IE                        VGTVGGGTQLASQSACLNLLGVKGA+++  G+NS
Sbjct: 547 IE------------------------VGTVGGGTQLASQSACLNLLGVKGASKDVAGANS 606

BLAST of Lsi10G000120 vs. ExPASy Swiss-Prot
Match: U5JCC6 (3-hydroxy-3-methylglutaryl coenzyme A reductase 2-B OS=Panax ginseng OX=4054 GN=HMGR2 PE=2 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 9.9e-243
Identity = 447/623 (71.75%), Postives = 505/623 (81.06%), Query Frame = 0

Query: 42  RPPPPKLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFF 101
           RP    LTA EP                + Q+ H  ++KASD LPLPLYLTN  FFT+FF
Sbjct: 6   RPVTKTLTAGEP---------------LKSQNQHSSSLKASDALPLPLYLTNGLFFTMFF 65

Query: 102 SVVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDV 161
           SV+YFLL RWREKIR+S PLHVVTLSE+ A+   +AS IYLLGFFGI FVQS+ RPS D 
Sbjct: 66  SVMYFLLHRWREKIRNSVPLHVVTLSELAALVLLVASVIYLLGFFGIGFVQSLIRPSPDS 125

Query: 162 WTSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPK--------VVDPVPVSIDLT 221
           W   +D+  +++ED+R  PC A IDCS+P      + VP+        VV+    S   T
Sbjct: 126 WDILEDDNAILEEDSRCEPCAAAIDCSLPPNLKIARMVPQKHKSAFADVVEEQKQSASAT 185

Query: 222 --EEDEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYAS 281
             +EDEEI+ +VVAGTTPSYSLESKLGDC +AAAIRREALQR+TGKSL GLPL+GFDY S
Sbjct: 186 IIDEDEEIINAVVAGTTPSYSLESKLGDCLKAAAIRREALQRITGKSLDGLPLDGFDYES 245

Query: 282 ILGQCCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANS 341
           ILGQCCEMP+GYVQ+PVGIAGPLLL+  EYSVPMATTEGCLVASTNRGCKAI  SGGA S
Sbjct: 246 ILGQCCEMPVGYVQVPVGIAGPLLLNETEYSVPMATTEGCLVASTNRGCKAIYASGGATS 305

Query: 342 VLLRDAMTRAPVMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGK 401
           VLL+D MTRAPV+RF +AKRAAELK ++EDP+NF+TLA VFNKSSRFGRLQ IKCAIAGK
Sbjct: 306 VLLKDGMTRAPVVRFGSAKRAAELKFFLEDPMNFETLALVFNKSSRFGRLQGIKCAIAGK 365

Query: 402 NLYMRFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEG 461
           NLYMRF+CSTGDAMGMNMVSKGVQNVLDFLQ+DFPDMDV+GISGNYCSDKKPAAVNWIEG
Sbjct: 366 NLYMRFTCSTGDAMGMNMVSKGVQNVLDFLQNDFPDMDVMGISGNYCSDKKPAAVNWIEG 425

Query: 462 RGKSVVCEVIIKGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIF 521
           RGKSVVCE IIK +VVKKVLKT+V ALVELNMLKNL GSA+AGALGGFNAHASNIVSA++
Sbjct: 426 RGKSVVCEAIIKEEVVKKVLKTNVAALVELNMLKNLAGSAVAGALGGFNAHASNIVSAVY 485

Query: 522 IATGQDPAQNVESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISES 581
           +A GQDPAQN+ESSHCITMMEAVNDG+DLH+SVTMPSIE                     
Sbjct: 486 VAAGQDPAQNIESSHCITMMEAVNDGKDLHISVTMPSIE--------------------- 545

Query: 582 KQLVGTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAIS 641
              VGTVGGGTQLASQSACLNLLGVKGA++E+PG NSRLLASIVAGSVLAGELSLMSA++
Sbjct: 546 ---VGTVGGGTQLASQSACLNLLGVKGASKESPGPNSRLLASIVAGSVLAGELSLMSALA 589

Query: 642 AGHLVRSHMKYNRSSRDVTKASS 655
           AG LV+SHMK+NRSS+DV+K SS
Sbjct: 606 AGQLVKSHMKFNRSSKDVSKLSS 589

BLAST of Lsi10G000120 vs. ExPASy Swiss-Prot
Match: A0A0A1C930 (3-hydroxy-3-methylglutaryl coenzyme A reductase 2-A OS=Panax ginseng OX=4054 GN=HMGR2 PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 3.2e-241
Identity = 446/619 (72.05%), Postives = 499/619 (80.61%), Query Frame = 0

Query: 49  TADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYFLL 108
           TA EPP               +QQH      KASD LPLPLYLTN  FFT+FFSV+YFLL
Sbjct: 18  TAGEPP-------------KSQQQHP-----KASDALPLPLYLTNGLFFTMFFSVMYFLL 77

Query: 109 TRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSEDDE 168
            RWREKIR+STPLHVVTLSE+ A+   +AS IYLLGFFGI FV+S+ RPS D W   +D+
Sbjct: 78  HRWREKIRNSTPLHVVTLSELAALVLLMASVIYLLGFFGIGFVRSVIRPSPDAWDILEDD 137

Query: 169 VVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPK-------------VVDPVPVSIDLTEE 228
             + +ED+R+ PC   IDCS+P     +  VP+             V  P      LTEE
Sbjct: 138 NAINEEDSRREPCAEAIDCSLPPKPKIVHMVPQKALNPKSAFADMMVEQPALAIAPLTEE 197

Query: 229 DEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQ 288
           DEEIVKSVV G  PSYSLESKLGDC +AA+IRREALQR+TGKSL+GLPL+GFDY SILGQ
Sbjct: 198 DEEIVKSVVTGKIPSYSLESKLGDCKKAASIRREALQRITGKSLAGLPLDGFDYKSILGQ 257

Query: 289 CCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLR 348
           CCEMP+GYVQIPVGIAGPLLL+  EYSVPMATTEGCLVASTNRGCKAI  SGGA SVLLR
Sbjct: 258 CCEMPVGYVQIPVGIAGPLLLNETEYSVPMATTEGCLVASTNRGCKAIYASGGATSVLLR 317

Query: 349 DAMTRAPVMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYM 408
           D MTRAPV+RF+T KRAAELK ++E+P+N+DT A VFNKSSRFGRLQ IKCA+AGKNLY+
Sbjct: 318 DGMTRAPVVRFSTVKRAAELKFFLEEPLNYDTPAHVFNKSSRFGRLQGIKCAVAGKNLYI 377

Query: 409 RFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKS 468
           RF+CSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDV+GISGNYCSDKKPAAVNWIEG GKS
Sbjct: 378 RFTCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVMGISGNYCSDKKPAAVNWIEGCGKS 437

Query: 469 VVCEVIIKGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATG 528
           VVCE IIK +VVKKVLKT+V ALVELNMLKNL GSA+AGALGGFNAHASNIVSA++I+TG
Sbjct: 438 VVCEAIIKEEVVKKVLKTNVAALVELNMLKNLAGSAVAGALGGFNAHASNIVSAVYISTG 497

Query: 529 QDPAQNVESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLV 588
           QDPAQNVESSHCITMMEAVN+G+DLH+SVTMPSIE                        V
Sbjct: 498 QDPAQNVESSHCITMMEAVNNGKDLHISVTMPSIE------------------------V 557

Query: 589 GTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHL 648
           GTVGGGTQLASQSACLNLLGVKGA++E+PGSNSRLLASIVAGSVLAGELSLMSA++AG L
Sbjct: 558 GTVGGGTQLASQSACLNLLGVKGASKESPGSNSRLLASIVAGSVLAGELSLMSALAAGQL 594

Query: 649 VRSHMKYNRSSRDVTKASS 655
           V+SHMKYNRSS+D+TK SS
Sbjct: 618 VKSHMKYNRSSKDITKLSS 594

BLAST of Lsi10G000120 vs. ExPASy TrEMBL
Match: A0A1S3BJ20 (3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucumis melo OX=3656 GN=LOC103490442 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 7.3e-310
Identity = 562/609 (92.28%), Postives = 575/609 (94.42%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           KL +DEPPV+SM KN LKVKMVER QHVHDDA+KASDVLPLP+YLTNAAFFTLFFSVVYF
Sbjct: 14  KLPSDEPPVKSMDKNPLKVKMVER-QHVHDDAVKASDVLPLPIYLTNAAFFTLFFSVVYF 73

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 74  LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 133

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVV+IKEDTRKVPCGAGIDCSIPILAPP+ SVPKVVDP+PVSIDLTEEDE+IVKSVV G
Sbjct: 134 DEVVMIKEDTRKVPCGAGIDCSIPILAPPMPSVPKVVDPLPVSIDLTEEDEDIVKSVVDG 193

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 194 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 253

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF
Sbjct: 254 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 313

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVEDP NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 314 ATAKRAAELKFYVEDPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 373

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV +KGDV
Sbjct: 374 MNMVSKGVQNVLDFLQNDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTVKGDV 433

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           V+KVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAI+IATGQDPAQNVESSH
Sbjct: 434 VRKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIYIATGQDPAQNVESSH 493

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 494 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 553

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVKGANREAPGSNSRLLA+IVA SVLAGELSLMSAISAGHLVRSHMKYNRSS
Sbjct: 554 QSACLNLLGVKGANREAPGSNSRLLATIVAASVLAGELSLMSAISAGHLVRSHMKYNRSS 597

Query: 647 RDVTKASSS 656
           RDVTKASSS
Sbjct: 614 RDVTKASSS 597

BLAST of Lsi10G000120 vs. ExPASy TrEMBL
Match: A0A0A0KA96 (3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucumis sativus OX=3659 GN=Csa_6G117710 PE=3 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 3.1e-308
Identity = 560/609 (91.95%), Postives = 574/609 (94.25%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           KLTADEPPV+SM KN LKVKM+ER QHVHDDA+KASDVLPLP+YLTNAAFFTLFFSVVYF
Sbjct: 82  KLTADEPPVKSMDKNPLKVKMLER-QHVHDDAVKASDVLPLPIYLTNAAFFTLFFSVVYF 141

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 142 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 201

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVV+IKEDTRKVPCGAGIDCSIPILAPP+ SVPKVVDP+PVSIDLTEEDEEIVKSVV G
Sbjct: 202 DEVVIIKEDTRKVPCGAGIDCSIPILAPPMPSVPKVVDPLPVSIDLTEEDEEIVKSVVDG 261

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           +TPSYSLESKLGDCGRAAAIRR ALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 262 STPSYSLESKLGDCGRAAAIRRVALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 321

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIM+SGGANSVLLRDAMTRAPVMRF
Sbjct: 322 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMISGGANSVLLRDAMTRAPVMRF 381

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVEDP NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 382 ATAKRAAELKFYVEDPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 441

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV IKGDV
Sbjct: 442 MNMVSKGVQNVLDFLQNDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTIKGDV 501

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           V+KVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAI+IATGQDPAQNVESSH
Sbjct: 502 VRKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIYIATGQDPAQNVESSH 561

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 562 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 621

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVKGANREAPGSNSRLLA+IVA SVLAGELSLMSAISAG LVRSHMKYNRSS
Sbjct: 622 QSACLNLLGVKGANREAPGSNSRLLATIVAASVLAGELSLMSAISAGQLVRSHMKYNRSS 665

Query: 647 RDVTKASSS 656
           RD+TKASSS
Sbjct: 682 RDITKASSS 665

BLAST of Lsi10G000120 vs. ExPASy TrEMBL
Match: A0A6J1JA10 (3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucurbita maxima OX=3661 GN=LOC111483921 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 9.8e-302
Identity = 550/608 (90.46%), Postives = 566/608 (93.09%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           K+T  EP VESM KNSLKVKMVE  QH  DDA+KA DVLPLP+YLTNAAFFTLFFSVVYF
Sbjct: 14  KVTVGEPAVESMEKNSLKVKMVE--QHHDDDAVKACDVLPLPIYLTNAAFFTLFFSVVYF 73

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASF+YLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 74  LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFVYLLGFFGIDFVQSIFRPSHDVWTSED 133

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVV+IKEDTRKVPCGAGIDCSIPILAPPL SVPKVVD VPVS+DLTEEDEEIVKSVVAG
Sbjct: 134 DEVVMIKEDTRKVPCGAGIDCSIPILAPPLPSVPKVVDLVPVSVDLTEEDEEIVKSVVAG 193

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           TTPSY LESKLGDC RAAAIR EALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 194 TTPSYLLESKLGDCRRAAAIRHEALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 253

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYS+PMATTEGCLVASTNRGCKAIMMSGGANSVLLRD+MTRAPV+RF
Sbjct: 254 PVGIAGPLLLDGKEYSIPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDSMTRAPVVRF 313

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVE+P NFDTLA+VFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 314 ATAKRAAELKFYVEEPANFDTLAAVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 373

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV+IKGDV
Sbjct: 374 MNMVSKGVQNVLDFLQTDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVVIKGDV 433

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           VKKVLKT+VQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH
Sbjct: 434 VKKVLKTNVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 493

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 494 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 553

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVKGANREAPGSN+RLLA+IVAGSVLAGELSLMSAISAG LVRSHMKYNRSS
Sbjct: 554 QSACLNLLGVKGANREAPGSNARLLATIVAGSVLAGELSLMSAISAGQLVRSHMKYNRSS 595

Query: 647 RDVTKASS 655
           RDVT ASS
Sbjct: 614 RDVTSASS 595

BLAST of Lsi10G000120 vs. ExPASy TrEMBL
Match: A0A6J1G7U3 (3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucurbita moschata OX=3662 GN=LOC111451663 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 6.3e-301
Identity = 544/613 (88.74%), Postives = 569/613 (92.82%), Query Frame = 0

Query: 43  PPPPKLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFS 102
           PP    +AD+ PV+SM KN LKVKM+E++ H   DA+KASDVLPLP+YLTNAAFFTLFFS
Sbjct: 10  PPAKNFSADQQPVKSMAKNPLKVKMMEQRDH---DAVKASDVLPLPIYLTNAAFFTLFFS 69

Query: 103 VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVW 162
           VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAF+ASFIYLLGFFGIDFVQSIFRPSHDVW
Sbjct: 70  VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFVASFIYLLGFFGIDFVQSIFRPSHDVW 129

Query: 163 TSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKS 222
           TSEDDEVVL++EDTRKVPCGAGIDC++PILA P+ SVPKVVDPVPVS DLTEEDEEIVKS
Sbjct: 130 TSEDDEVVLVREDTRKVPCGAGIDCAMPILASPVPSVPKVVDPVPVSFDLTEEDEEIVKS 189

Query: 223 VVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIG 282
           VVAG TPSYSLESKLGDC RAAAIRRE+LQR+TGKSLSGLPLEGFDYASILGQCCEMP+G
Sbjct: 190 VVAGATPSYSLESKLGDCARAAAIRRESLQRITGKSLSGLPLEGFDYASILGQCCEMPVG 249

Query: 283 YVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP 342
           YVQ+PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP
Sbjct: 250 YVQVPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP 309

Query: 343 VMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG 402
           VMRFATAKRAAELK YVE+P NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG
Sbjct: 310 VMRFATAKRAAELKFYVEEPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG 369

Query: 403 DAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVII 462
           DAMGMNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV I
Sbjct: 370 DAMGMNMVSKGVQNVLDFLQTDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTI 429

Query: 463 KGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV 522
            GDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV
Sbjct: 430 TGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV 489

Query: 523 ESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGT 582
           ESSHCITMMEAVN+GQDLHVSVTMPSIE                        VGTVGGGT
Sbjct: 490 ESSHCITMMEAVNNGQDLHVSVTMPSIE------------------------VGTVGGGT 549

Query: 583 QLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKY 642
           QLASQSACLNLLGVKGA+REAPGSN+RLLA+IVAGSVLAGELSLMSAISAGHLVRSHMKY
Sbjct: 550 QLASQSACLNLLGVKGASREAPGSNARLLATIVAGSVLAGELSLMSAISAGHLVRSHMKY 595

Query: 643 NRSSRDVTKASSS 656
           NRSSRDVT+ASSS
Sbjct: 610 NRSSRDVTRASSS 595

BLAST of Lsi10G000120 vs. ExPASy TrEMBL
Match: A0A6J1I5F6 (3-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucurbita maxima OX=3661 GN=LOC111469777 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.1e-300
Identity = 544/613 (88.74%), Postives = 568/613 (92.66%), Query Frame = 0

Query: 43  PPPPKLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFS 102
           PP    +AD+ PV+SM KN LKVKM+E++ H   DA+KASDVLPLP+YLTNAAFFTLFFS
Sbjct: 10  PPAKNFSADQQPVKSMAKNPLKVKMMEQRDH---DAVKASDVLPLPIYLTNAAFFTLFFS 69

Query: 103 VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVW 162
           VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAF+ASFIYLLGFFGIDFVQSIFRPSHDVW
Sbjct: 70  VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFVASFIYLLGFFGIDFVQSIFRPSHDVW 129

Query: 163 TSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKS 222
           TSEDDEVVL++EDTRKVPCGAGIDC++PIL  P+ SVPKV DPVPVS DLTEEDEEIVKS
Sbjct: 130 TSEDDEVVLVREDTRKVPCGAGIDCAMPILTSPVPSVPKVADPVPVSFDLTEEDEEIVKS 189

Query: 223 VVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIG 282
           VVAGTTPSYSLESKLGDC RAAAIRRE+LQR+TGKSLSGLPLEGFDYASILGQCCEMP+G
Sbjct: 190 VVAGTTPSYSLESKLGDCARAAAIRRESLQRITGKSLSGLPLEGFDYASILGQCCEMPVG 249

Query: 283 YVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP 342
           YVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP
Sbjct: 250 YVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP 309

Query: 343 VMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG 402
           VMRFATAKRAAELK YVE+P NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG
Sbjct: 310 VMRFATAKRAAELKFYVEEPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG 369

Query: 403 DAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVII 462
           DAMGMNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV I
Sbjct: 370 DAMGMNMVSKGVQNVLDFLQTDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTI 429

Query: 463 KGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV 522
            GDVVKKVLKT+VQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV
Sbjct: 430 TGDVVKKVLKTNVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV 489

Query: 523 ESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGT 582
           ESSHCITMMEAVN+GQDLHVSVTMPSIE                        VGTVGGGT
Sbjct: 490 ESSHCITMMEAVNNGQDLHVSVTMPSIE------------------------VGTVGGGT 549

Query: 583 QLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKY 642
           QLASQSACLNLLGVKGANREAPGSN+RLLA+IVAGSVLAGELSLMSAISAGHLVRSHMKY
Sbjct: 550 QLASQSACLNLLGVKGANREAPGSNARLLATIVAGSVLAGELSLMSAISAGHLVRSHMKY 595

Query: 643 NRSSRDVTKASSS 656
           NRSSRDVT+ASSS
Sbjct: 610 NRSSRDVTRASSS 595

BLAST of Lsi10G000120 vs. NCBI nr
Match: XP_008448157.1 (PREDICTED: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucumis melo])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.5e-309
Identity = 562/609 (92.28%), Postives = 575/609 (94.42%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           KL +DEPPV+SM KN LKVKMVER QHVHDDA+KASDVLPLP+YLTNAAFFTLFFSVVYF
Sbjct: 14  KLPSDEPPVKSMDKNPLKVKMVER-QHVHDDAVKASDVLPLPIYLTNAAFFTLFFSVVYF 73

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 74  LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 133

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVV+IKEDTRKVPCGAGIDCSIPILAPP+ SVPKVVDP+PVSIDLTEEDE+IVKSVV G
Sbjct: 134 DEVVMIKEDTRKVPCGAGIDCSIPILAPPMPSVPKVVDPLPVSIDLTEEDEDIVKSVVDG 193

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 194 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 253

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF
Sbjct: 254 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 313

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVEDP NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 314 ATAKRAAELKFYVEDPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 373

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV +KGDV
Sbjct: 374 MNMVSKGVQNVLDFLQNDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTVKGDV 433

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           V+KVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAI+IATGQDPAQNVESSH
Sbjct: 434 VRKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIYIATGQDPAQNVESSH 493

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 494 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 553

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVKGANREAPGSNSRLLA+IVA SVLAGELSLMSAISAGHLVRSHMKYNRSS
Sbjct: 554 QSACLNLLGVKGANREAPGSNSRLLATIVAASVLAGELSLMSAISAGHLVRSHMKYNRSS 597

Query: 647 RDVTKASSS 656
           RDVTKASSS
Sbjct: 614 RDVTKASSS 597

BLAST of Lsi10G000120 vs. NCBI nr
Match: XP_038876645.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Benincasa hispida])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.5e-309
Identity = 567/609 (93.10%), Postives = 572/609 (93.92%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           KLTADEPPVESM KNSLKVKMVER QHVHDDA+K SDVLPLP+Y TNAAFFTLFFSVVYF
Sbjct: 15  KLTADEPPVESMDKNSLKVKMVER-QHVHDDAVKTSDVLPLPIYFTNAAFFTLFFSVVYF 74

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 75  LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 134

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSV KVVD VPVSIDLTEEDEEIVKSVV G
Sbjct: 135 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVTKVVDQVPVSIDLTEEDEEIVKSVVTG 194

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 195 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 254

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF
Sbjct: 255 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 314

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVEDP NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 315 ATAKRAAELKFYVEDPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 374

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV IK DV
Sbjct: 375 MNMVSKGVQNVLDFLQNDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTIKDDV 434

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           VKKVLKTDVQAL ELNMLKNLTGSAMAGALGGFNAHASNIVSAI+IATGQDPAQNVESSH
Sbjct: 435 VKKVLKTDVQALGELNMLKNLTGSAMAGALGGFNAHASNIVSAIYIATGQDPAQNVESSH 494

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 495 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 554

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVK ANREAPGSNSRLLA+IVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS
Sbjct: 555 QSACLNLLGVKEANREAPGSNSRLLATIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 598

Query: 647 RDVTKASSS 656
           RDVTKASSS
Sbjct: 615 RDVTKASSS 598

BLAST of Lsi10G000120 vs. NCBI nr
Match: XP_004139979.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 [Cucumis sativus] >KAE8646818.1 hypothetical protein Csa_005765 [Cucumis sativus])

HSP 1 Score: 1067.0 bits (2758), Expect = 6.5e-308
Identity = 560/609 (91.95%), Postives = 574/609 (94.25%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           KLTADEPPV+SM KN LKVKM+ER QHVHDDA+KASDVLPLP+YLTNAAFFTLFFSVVYF
Sbjct: 14  KLTADEPPVKSMDKNPLKVKMLER-QHVHDDAVKASDVLPLPIYLTNAAFFTLFFSVVYF 73

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 74  LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 133

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVV+IKEDTRKVPCGAGIDCSIPILAPP+ SVPKVVDP+PVSIDLTEEDEEIVKSVV G
Sbjct: 134 DEVVIIKEDTRKVPCGAGIDCSIPILAPPMPSVPKVVDPLPVSIDLTEEDEEIVKSVVDG 193

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           +TPSYSLESKLGDCGRAAAIRR ALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 194 STPSYSLESKLGDCGRAAAIRRVALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 253

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIM+SGGANSVLLRDAMTRAPVMRF
Sbjct: 254 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMISGGANSVLLRDAMTRAPVMRF 313

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVEDP NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 314 ATAKRAAELKFYVEDPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 373

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV IKGDV
Sbjct: 374 MNMVSKGVQNVLDFLQNDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTIKGDV 433

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           V+KVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAI+IATGQDPAQNVESSH
Sbjct: 434 VRKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIYIATGQDPAQNVESSH 493

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 494 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 553

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVKGANREAPGSNSRLLA+IVA SVLAGELSLMSAISAG LVRSHMKYNRSS
Sbjct: 554 QSACLNLLGVKGANREAPGSNSRLLATIVAASVLAGELSLMSAISAGQLVRSHMKYNRSS 597

Query: 647 RDVTKASSS 656
           RD+TKASSS
Sbjct: 614 RDITKASSS 597

BLAST of Lsi10G000120 vs. NCBI nr
Match: XP_022986061.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita maxima])

HSP 1 Score: 1045.4 bits (2702), Expect = 2.0e-301
Identity = 550/608 (90.46%), Postives = 566/608 (93.09%), Query Frame = 0

Query: 47  KLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFSVVYF 106
           K+T  EP VESM KNSLKVKMVE  QH  DDA+KA DVLPLP+YLTNAAFFTLFFSVVYF
Sbjct: 14  KVTVGEPAVESMEKNSLKVKMVE--QHHDDDAVKACDVLPLPIYLTNAAFFTLFFSVVYF 73

Query: 107 LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVWTSED 166
           LLTRWREKIRSSTPLHVVTLSEMVAISAFIASF+YLLGFFGIDFVQSIFRPSHDVWTSED
Sbjct: 74  LLTRWREKIRSSTPLHVVTLSEMVAISAFIASFVYLLGFFGIDFVQSIFRPSHDVWTSED 133

Query: 167 DEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKSVVAG 226
           DEVV+IKEDTRKVPCGAGIDCSIPILAPPL SVPKVVD VPVS+DLTEEDEEIVKSVVAG
Sbjct: 134 DEVVMIKEDTRKVPCGAGIDCSIPILAPPLPSVPKVVDLVPVSVDLTEEDEEIVKSVVAG 193

Query: 227 TTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 286
           TTPSY LESKLGDC RAAAIR EALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI
Sbjct: 194 TTPSYLLESKLGDCRRAAAIRHEALQRVTGKSLSGLPLEGFDYASILGQCCEMPIGYVQI 253

Query: 287 PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAPVMRF 346
           PVGIAGPLLLDGKEYS+PMATTEGCLVASTNRGCKAIMMSGGANSVLLRD+MTRAPV+RF
Sbjct: 254 PVGIAGPLLLDGKEYSIPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDSMTRAPVVRF 313

Query: 347 ATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 406
           ATAKRAAELK YVE+P NFDTLA+VFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG
Sbjct: 314 ATAKRAAELKFYVEEPANFDTLAAVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTGDAMG 373

Query: 407 MNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVIIKGDV 466
           MNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV+IKGDV
Sbjct: 374 MNMVSKGVQNVLDFLQTDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVVIKGDV 433

Query: 467 VKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 526
           VKKVLKT+VQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH
Sbjct: 434 VKKVLKTNVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNVESSH 493

Query: 527 CITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGTQLAS 586
           CITMMEAVNDGQDLHVSVTMPSIE                        VGTVGGGTQLAS
Sbjct: 494 CITMMEAVNDGQDLHVSVTMPSIE------------------------VGTVGGGTQLAS 553

Query: 587 QSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKYNRSS 646
           QSACLNLLGVKGANREAPGSN+RLLA+IVAGSVLAGELSLMSAISAG LVRSHMKYNRSS
Sbjct: 554 QSACLNLLGVKGANREAPGSNARLLATIVAGSVLAGELSLMSAISAGQLVRSHMKYNRSS 595

Query: 647 RDVTKASS 655
           RDVT ASS
Sbjct: 614 RDVTSASS 595

BLAST of Lsi10G000120 vs. NCBI nr
Match: KAG6604981.1 (3-hydroxy-3-methylglutaryl-coenzyme A reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1043.1 bits (2696), Expect = 1.0e-300
Identity = 544/613 (88.74%), Postives = 569/613 (92.82%), Query Frame = 0

Query: 43  PPPPKLTADEPPVESMVKNSLKVKMVERQQHVHDDAIKASDVLPLPLYLTNAAFFTLFFS 102
           PP    +AD+ PV+SM KN LKVKM+E++ H   DA+KASDVLPLP+YLTNAAFFTLFFS
Sbjct: 10  PPAKNFSADQQPVKSMAKNPLKVKMMEQRDH---DAVKASDVLPLPIYLTNAAFFTLFFS 69

Query: 103 VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASFIYLLGFFGIDFVQSIFRPSHDVW 162
           VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAF+ASFIYLLGFFGIDFVQSIFRPSHDVW
Sbjct: 70  VVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFVASFIYLLGFFGIDFVQSIFRPSHDVW 129

Query: 163 TSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQSVPKVVDPVPVSIDLTEEDEEIVKS 222
           TSEDDEVVL++EDTRKVPCG GIDC++PILA P+ SVPKVVDPVPVS DLTEEDEEIVKS
Sbjct: 130 TSEDDEVVLVREDTRKVPCGTGIDCAMPILASPVPSVPKVVDPVPVSFDLTEEDEEIVKS 189

Query: 223 VVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGKSLSGLPLEGFDYASILGQCCEMPIG 282
           VVAGTTPSYSLESKLGDC RAAAIRRE+LQR+TGKSLSGLPLEGFDYASILGQCCEMP+G
Sbjct: 190 VVAGTTPSYSLESKLGDCARAAAIRRESLQRITGKSLSGLPLEGFDYASILGQCCEMPVG 249

Query: 283 YVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP 342
           YVQ+PVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP
Sbjct: 250 YVQVPVGIAGPLLLDGKEYSVPMATTEGCLVASTNRGCKAIMMSGGANSVLLRDAMTRAP 309

Query: 343 VMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG 402
           VMRFATAKRAAELK YVE+P NFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG
Sbjct: 310 VMRFATAKRAAELKFYVEEPANFDTLASVFNKSSRFGRLQSIKCAIAGKNLYMRFSCSTG 369

Query: 403 DAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVII 462
           DAMGMNMVSKGVQNVLDFLQ+DFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEV I
Sbjct: 370 DAMGMNMVSKGVQNVLDFLQTDFPDMDVIGISGNYCSDKKPAAVNWIEGRGKSVVCEVTI 429

Query: 463 KGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV 522
            GDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV
Sbjct: 430 TGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALGGFNAHASNIVSAIFIATGQDPAQNV 489

Query: 523 ESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFHNNLLYDLIVEISESKQLVGTVGGGT 582
           ESSHCITMMEAVN+GQDLHVSVTMPSIE                        VGTVGGGT
Sbjct: 490 ESSHCITMMEAVNNGQDLHVSVTMPSIE------------------------VGTVGGGT 549

Query: 583 QLASQSACLNLLGVKGANREAPGSNSRLLASIVAGSVLAGELSLMSAISAGHLVRSHMKY 642
           QLASQSACLNLLGVKGA+REAPGSN+RLLA+IVAGSVLAGELSLMSAISAGHLVRSHMKY
Sbjct: 550 QLASQSACLNLLGVKGASREAPGSNARLLATIVAGSVLAGELSLMSAISAGHLVRSHMKY 595

Query: 643 NRSSRDVTKASSS 656
           NRSSRDVT+ASSS
Sbjct: 610 NRSSRDVTRASSS 595

BLAST of Lsi10G000120 vs. TAIR 10
Match: AT1G76490.1 (hydroxy methylglutaryl CoA reductase 1 )

HSP 1 Score: 792.7 bits (2046), Expect = 2.2e-229
Identity = 419/578 (72.49%), Postives = 480/578 (83.04%), Query Frame = 0

Query: 80  KASDVLPLPLYLTNAAFFTLFFSVVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASF 139
           KASD LPLPLYLTNA FFTLFFSV Y+LL RWR+KIR +TPLHVVT++E+ AI A IASF
Sbjct: 96  KASDALPLPLYLTNAVFFTLFFSVAYYLLHRWRDKIRYNTPLHVVTITELGAIIALIASF 155

Query: 140 IYLLGFFGIDFVQS-IFRPSHDVWTSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQS 199
           IYLLGFFGIDFVQS I R S D W   D     I +D  ++     + CS P    P+ S
Sbjct: 156 IYLLGFFGIDFVQSFISRASGDAWDLAD----TIDDDDHRL-----VTCSPP---TPIVS 215

Query: 200 VPKVVDPVP-VSIDLTEEDEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGK 259
           V K+ +P P V+  L EEDEEIVKSV+ G  PSYSLES+LGDC RAA+IRREALQRVTG+
Sbjct: 216 VAKLPNPEPIVTESLPEEDEEIVKSVIDGVIPSYSLESRLGDCKRAASIRREALQRVTGR 275

Query: 260 SLSGLPLEGFDYASILGQCCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTN 319
           S+ GLPL+GFDY SILGQCCEMP+GY+QIPVGIAGPLLLDG EYSVPMATTEGCLVASTN
Sbjct: 276 SIEGLPLDGFDYESILGQCCEMPVGYIQIPVGIAGPLLLDGYEYSVPMATTEGCLVASTN 335

Query: 320 RGCKAIMMSGGANSVLLRDAMTRAPVMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSR 379
           RGCKA+ +SGGA S +L+D MTRAPV+RFA+A+RA+ELK ++E+P NFDTLA VFN+SSR
Sbjct: 336 RGCKAMFISGGATSTVLKDGMTRAPVVRFASARRASELKFFLENPENFDTLAVVFNRSSR 395

Query: 380 FGRLQSIKCAIAGKNLYMRFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNY 439
           F RLQS+KC IAGKN Y+RF CSTGDAMGMNMVSKGVQNVL++L  DFPDMDVIGISGN+
Sbjct: 396 FARLQSVKCTIAGKNAYVRFCCSTGDAMGMNMVSKGVQNVLEYLTDDFPDMDVIGISGNF 455

Query: 440 CSDKKPAAVNWIEGRGKSVVCEVIIKGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALG 499
           CSDKKPAAVNWIEGRGKSVVCE +I+G++V KVLKT V ALVELNMLKNL GSA+AG+LG
Sbjct: 456 CSDKKPAAVNWIEGRGKSVVCEAVIRGEIVNKVLKTSVAALVELNMLKNLAGSAVAGSLG 515

Query: 500 GFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFH 559
           GFNAHASNIVSA+FIATGQDPAQNVESS CITMMEA+NDG+D+H+SVTMPSIE       
Sbjct: 516 GFNAHASNIVSAVFIATGQDPAQNVESSQCITMMEAINDGKDIHISVTMPSIE------- 575

Query: 560 NNLLYDLIVEISESKQLVGTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLASIVAG 619
                            VGTVGGGTQLASQSACLNLLGVKGA+ E+PG N+R LA+IVAG
Sbjct: 576 -----------------VGTVGGGTQLASQSACLNLLGVKGASTESPGMNARRLATIVAG 635

Query: 620 SVLAGELSLMSAISAGHLVRSHMKYNRSSRDVTKASSS 656
           +VLAGELSLMSAI+AG LVRSHMKYNRSSRD++ A+++
Sbjct: 636 AVLAGELSLMSAIAAGQLVRSHMKYNRSSRDISGATTT 637

BLAST of Lsi10G000120 vs. TAIR 10
Match: AT2G17370.1 (3-hydroxy-3-methylglutaryl-CoA reductase 2 )

HSP 1 Score: 741.9 bits (1914), Expect = 4.5e-214
Identity = 401/576 (69.62%), Postives = 457/576 (79.34%), Query Frame = 0

Query: 80  KASDVLPLPLYLTNAAFFTLFFSVVYFLLTRWREKIRSSTPLHVVTLSEMVAISAFIASF 139
           KASD LPLPLYLTN  F +LFF+ VYFLL+RWREKIR+STPLHVV LSE+ A+  F+ASF
Sbjct: 28  KASDALPLPLYLTNTFFLSLFFATVYFLLSRWREKIRNSTPLHVVDLSEICALIGFVASF 87

Query: 140 IYLLGFFGIDFVQSIFRPS--HDVWTSEDDEVVLIKEDTRKVPCGAGIDCSIPILAPPLQ 199
           IYLLGF GID    IFR S   DVW ++             +PC   +DC        L 
Sbjct: 88  IYLLGFCGIDL---IFRSSSDDDVWVNDG-----------MIPCNQSLDC-----REVLP 147

Query: 200 SVPKVVDPVPVSIDLTEEDEEIVKSVVAGTTPSYSLESKLGDCGRAAAIRREALQRVTGK 259
             P  VDP   S   + EDEEIVK V+ GT PSYSLE+KLGDC RAAAIRREA+QR+TGK
Sbjct: 148 IKPNSVDPPRESELDSVEDEEIVKLVIDGTIPSYSLETKLGDCKRAAAIRREAVQRITGK 207

Query: 260 SLSGLPLEGFDYASILGQCCEMPIGYVQIPVGIAGPLLLDGKEYSVPMATTEGCLVASTN 319
           SL+GLPLEGFDY SILGQCCEMP+GYVQIPVGIAGPLLLDG EYSVPMATTEGCLVASTN
Sbjct: 208 SLTGLPLEGFDYNSILGQCCEMPVGYVQIPVGIAGPLLLDGVEYSVPMATTEGCLVASTN 267

Query: 320 RGCKAIMMSGGANSVLLRDAMTRAPVMRFATAKRAAELKMYVEDPVNFDTLASVFNKSSR 379
           RG KAI +SGGA SVL++DAMTRAPV+RF +A+RAA +  Y++DP NF+ L+ +FNKSSR
Sbjct: 268 RGFKAIHLSGGAFSVLVKDAMTRAPVVRFPSARRAALVMFYLQDPSNFERLSLIFNKSSR 327

Query: 380 FGRLQSIKCAIAGKNLYMRFSCSTGDAMGMNMVSKGVQNVLDFLQSDFPDMDVIGISGNY 439
           F RLQSI C IAG+NLY RF+CSTGDAMGMNMVSKGVQNVLDF++S+FPDMDVIGISGNY
Sbjct: 328 FARLQSITCTIAGRNLYPRFACSTGDAMGMNMVSKGVQNVLDFVKSEFPDMDVIGISGNY 387

Query: 440 CSDKKPAAVNWIEGRGKSVVCEVIIKGDVVKKVLKTDVQALVELNMLKNLTGSAMAGALG 499
           CSDKK +AVNWIEGRGK VVCE  IK ++V+KVLKT V+ALVELN LKNL GSAMAG+LG
Sbjct: 388 CSDKKASAVNWIEGRGKHVVCEAFIKAEIVEKVLKTSVEALVELNTLKNLVGSAMAGSLG 447

Query: 500 GFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEAVNDGQDLHVSVTMPSIEVGEFTFH 559
           GFNAH+SNIVSA+FIATGQDPAQNVESSHC+TM+  + DG DLH+SV+MP IE       
Sbjct: 448 GFNAHSSNIVSAVFIATGQDPAQNVESSHCMTMI--LPDGDDLHISVSMPCIE------- 507

Query: 560 NNLLYDLIVEISESKQLVGTVGGGTQLASQSACLNLLGVKGANREAPGSNSRLLASIVAG 619
                            VGTVGGGTQLASQ+ACLNLLGVKG+N E PGSN++ LA IVAG
Sbjct: 508 -----------------VGTVGGGTQLASQAACLNLLGVKGSNNEKPGSNAQQLARIVAG 558

Query: 620 SVLAGELSLMSAISAGHLVRSHMKYNRSSRDVTKAS 654
           SVLAGELSLMSAI+AG LV+SHMKYNRSSRD+  +S
Sbjct: 568 SVLAGELSLMSAIAAGQLVKSHMKYNRSSRDIGPSS 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O649666.9e-25276.873-hydroxy-3-methylglutaryl-coenzyme A reductase 1 OS=Gossypium hirsutum OX=3635 ... [more]
P480214.6e-24874.043-hydroxy-3-methylglutaryl-coenzyme A reductase OS=Camptotheca acuminata OX=1692... [more]
O649671.3e-24571.053-hydroxy-3-methylglutaryl-coenzyme A reductase 2 OS=Gossypium hirsutum OX=3635 ... [more]
U5JCC69.9e-24371.753-hydroxy-3-methylglutaryl coenzyme A reductase 2-B OS=Panax ginseng OX=4054 GN=... [more]
A0A0A1C9303.2e-24172.053-hydroxy-3-methylglutaryl coenzyme A reductase 2-A OS=Panax ginseng OX=4054 GN=... [more]
Match NameE-valueIdentityDescription
A0A1S3BJ207.3e-31092.283-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KA963.1e-30891.953-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A6J1JA109.8e-30290.463-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1G7U36.3e-30188.743-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1I5F61.1e-30088.743-hydroxy-3-methylglutaryl coenzyme A reductase OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_008448157.11.5e-30992.28PREDICTED: 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucumis melo][more]
XP_038876645.11.5e-30993.103-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Benincasa hispida][more]
XP_004139979.16.5e-30891.953-hydroxy-3-methylglutaryl-coenzyme A reductase 1 [Cucumis sativus] >KAE8646818.... [more]
XP_022986061.12.0e-30190.463-hydroxy-3-methylglutaryl-coenzyme A reductase 1-like [Cucurbita maxima][more]
KAG6604981.11.0e-30088.743-hydroxy-3-methylglutaryl-coenzyme A reductase 1, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT1G76490.12.2e-22972.49hydroxy methylglutaryl CoA reductase 1 [more]
AT2G17370.14.5e-21469.623-hydroxy-3-methylglutaryl-CoA reductase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002202Hydroxymethylglutaryl-CoA reductase, class I/IIPRINTSPR00071HMGCOARDTASEcoord: 276..297
score: 77.84
coord: 303..323
score: 91.87
coord: 434..459
score: 88.38
coord: 394..412
score: 82.35
coord: 539..560
score: 56.34
coord: 485..511
score: 78.32
IPR002202Hydroxymethylglutaryl-CoA reductase, class I/IIPFAMPF00368HMG-CoA_redcoord: 574..644
e-value: 5.4E-21
score: 75.0
coord: 244..552
e-value: 2.8E-116
score: 388.5
IPR002202Hydroxymethylglutaryl-CoA reductase, class I/IIPANTHERPTHR105723-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASEcoord: 563..654
coord: 69..553
IPR002202Hydroxymethylglutaryl-CoA reductase, class I/IIPROSITEPS50065HMG_COA_REDUCTASE_4coord: 216..644
score: 135.480469
IPR023282Hydroxymethylglutaryl-CoA reductase, N-terminalGENE3D1.10.3270.10coord: 212..286
e-value: 5.0E-22
score: 79.8
IPR023074Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain superfamilyGENE3D3.90.770.10coord: 556..647
e-value: 3.4E-21
score: 77.8
IPR023074Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain superfamilyGENE3D3.90.770.10coord: 287..552
e-value: 3.4E-116
score: 389.7
IPR009023Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain superfamilyGENE3D3.30.70.420coord: 337..453
e-value: 3.4E-116
score: 389.7
IPR009023Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain superfamilySUPERFAMILY55035NAD-binding domain of HMG-CoA reductasecoord: 338..453
NoneNo IPR availablePANTHERPTHR10572:SF443-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASEcoord: 563..654
coord: 69..553
IPR023076Hydroxymethylglutaryl-CoA reductase, class I/II, conserved sitePROSITEPS01192HMG_COA_REDUCTASE_3coord: 629..642
IPR023076Hydroxymethylglutaryl-CoA reductase, class I/II, conserved sitePROSITEPS00066HMG_COA_REDUCTASE_1coord: 396..410
IPR023076Hydroxymethylglutaryl-CoA reductase, class I/II, conserved sitePROSITEPS00318HMG_COA_REDUCTASE_2coord: 575..582
IPR004554Hydroxymethylglutaryl-CoA reductase, eukaryotic/archaeal typeCDDcd00643HMG-CoA_reductase_classIcoord: 217..644
e-value: 0.0
score: 680.045
IPR009029Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding domain superfamilySUPERFAMILY56542Substrate-binding domain of HMG-CoA reductasecoord: 213..634

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G000120.1Lsi10G000120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015936 coenzyme A metabolic process
biological_process GO:0008299 isoprenoid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005778 peroxisomal membrane
molecular_function GO:0004420 hydroxymethylglutaryl-CoA reductase (NADPH) activity
molecular_function GO:0005515 protein binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor