Lsi09G017750 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G017750
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPlant protein of unknown function (DUF247)
Locationchr09: 26497321 .. 26499757 (+)
RNA-Seq ExpressionLsi09G017750
SyntenyLsi09G017750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCATTATAAGGCATTGTGGAGATGAATATGATGCAGTCAACAGTAGGCAGCGAATTCTTTGGTGGAGACGATGATGAGTTTATTATTAAAGATATGGGTAAGGTTGTAAATGAGGTGAAAGAGCTTCTGAATAAAGTTGAGGATAATGTTTTGAAGGAGGAGTTACGTAAGATGCAAGAATTATCAAGGCAAAAGGGGAAAGCTTCAATCTACAAAATACCAGAAGAAATAAGAGAAGTTAATCCCAAAGCTTTCGAGCCACAGTTGGTGTCTTTTGGGCCATACCATTATGGGAAACCCAATTTGCTTGAAATGGAATTGGAAAAACAGAATGGGTATCTCCGTTTTAAAGCTAAAAACAATTTAGAATTTGAGTCCATTATTGTGGAGAGTGTGTCGAGCACTTTGAAACAGCTGTTGGGATCTTATGATAATCTTGAGGATAAATGGAAGCAAAATCCTGCTAAGTTCTTGAAGCTCATGATTGTGGATGGCTGTTTCATGCTGGATTTGATAATCAATGGATGTCCTCAATCGCTAGCTTCTATGATTGGGAATATAAAGCGGGACATGCTGTTGCTTGAGAATCAACTACCCTTCAACCTACTTCAACACCTCTATGATTTAGTGAACAACAACGGCCATAAGGTATAATTTTTTATATATAATTATTTAATAATTTTCATCCAATGTATTTATGTTTTGATTGAAATTTCATGATTTTGAGAGTCTTGAAATCTTGATTAGGGAAAATTTTGTTTTCCCTCCAAGAATATTCCGATTCCTCCCACTTTGACGAAATTTCGAGATTTCGATTTTTTTTAATGATTAATTATGCTAGTTAAGTTCAAAATTTTCAATTTTATGGTTTGATTACACATAATTCTATAGTTTTACACGCGAATGTCATTTTCGACAAATGATTATTATACATTTTTTATGTGCTACTTTTTTTAATTTCCAACTTTTCTCAATTTTTAGCTTTATTTTCGACTCAAAACTTTCATTTATAAAGAAATTGAATTTGAAAAAATCACACACACAAAAAAAAATCTATATTGATTTTTAAGCTTAATCAAACTTTTTTTTTAAAATTAAATTTTCACATCATTTCACCTTACAATTGAATTAATCCGAGTTTTTTCTTTTACCATCCAAAACTTTCATAATTTTTACATCATTTTACTTCAAAAACTTTTTAGGAAAATTTTCACAGATAGAAAAAATGTCAAACTATTTACAGAAAATAGCAAAAAAAACACTGATAGATATTGATAGACTTCTATCAGTATCTATCAGTGATAGATTTCTATCATTTCTATCGTGATAGACTTCTACCAGCATCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAATTTTTCTTATTTTTTTATTTTTAAAAATCTCCCAACTTTTTATTATTTTATAATTATTTTTTCATTTCTTTTTTAAATTTTTGCTCTCCTGTCAACAGTTGCTCAAACATTTACAATAGACATCTAAATATTATCTTATTTAATAGAAATTATGATTAAAAATAGTAAGTTATAATCTAATTAAGTCACAATCAAATTTTTATTAATTAATTATAACAATTTCTATAAATTTCCACAATTTTTTCGGACTTGATTATAGAGTATAGATGATGTGATTACACAGGATAAAGGGAGTCTGAGCTTGCTGATTTTGTACTGGATGGGGATACCGAAGGGAGGGGTAATAACGGGAAAATACTTGCACATTTTGGATATGCACAGAGCTTTAATGTTGTATCCTTCAATAGAAAAGACAGATGATTACAATAGTAGAAGCCGAAGAAGGTACAACCAGACGGAGGTGGAGTGTGGATCGGCAACACGACTTGGTAAATTAGGGATAGAATTTAAGGAAATTATTACAGATAACCTTATGGACGTGTCCTTCAACTTCAAAACAGGAGTGCTGAGTGTGCCACAGCTGAAGCTGGATCACACCACTCTACCAAATTTATTAAATATGATGACATTTGAGAAATTCCATGACCAGATTGGAAGTGAAGTAATTTCATTTGTGGTGTTTATGACAAATCTAATTGAAGCCGAAGAAGATGTGTCGCTGCTAATCTCAAAGAAAATATTAAGGAATGAACTTGATCAAGACCGTAAAGTTGTGTTACAATTGTTCAATCAGCTGTCAAAAGGACCAACCAAATGTTTGGATACACACATTTGTGAAGTATTAAACATGTTGAATAAGCATTGTAATAATCCTTGGACTATGTGGTGGGTAAATCTCAAAGACCCTCGAACTCAAGATCCATGGGTTATTATCTCCGTTGCTGCCGTTCTCTTTGGTTTTCTGCTCCAAATTATCCAAACCGTCTACGGAATTTATGGAATTTACCGCCCAAATCATTAACCACATTGTTTTTTTTCTTTTTTTTTTTTT

mRNA sequence

GCATTATAAGGCATTGTGGAGATGAATATGATGCAGTCAACAGTAGGCAGCGAATTCTTTGGTGGAGACGATGATGAGTTTATTATTAAAGATATGGGTAAGGTTGTAAATGAGGTGAAAGAGCTTCTGAATAAAGTTGAGGATAATGTTTTGAAGGAGGAGTTACGTAAGATGCAAGAATTATCAAGGCAAAAGGGGAAAGCTTCAATCTACAAAATACCAGAAGAAATAAGAGAAGTTAATCCCAAAGCTTTCGAGCCACAGTTGGTGTCTTTTGGGCCATACCATTATGGGAAACCCAATTTGCTTGAAATGGAATTGGAAAAACAGAATGGGTATCTCCGTTTTAAAGCTAAAAACAATTTAGAATTTGAGTCCATTATTGTGGAGAGTGTGTCGAGCACTTTGAAACAGCTGTTGGGATCTTATGATAATCTTGAGGATAAATGGAAGCAAAATCCTGCTAAGTTCTTGAAGCTCATGATTGTGGATGGCTGTTTCATGCTGGATTTGATAATCAATGGATGTCCTCAATCGCTAGCTTCTATGATTGGGAATATAAAGCGGGACATGCTGTTGCTTGAGAATCAACTACCCTTCAACCTACTTCAACACCTCTATGATTTAGTGAACAACAACGGCCATAAGGATAAAGGGAGTCTGAGCTTGCTGATTTTGTACTGGATGGGGATACCGAAGGGAGGGGTAATAACGGGAAAATACTTGCACATTTTGGATATGCACAGAGCTTTAATGTTGTATCCTTCAATAGAAAAGACAGATGATTACAATAGTAGAAGCCGAAGAAGGTACAACCAGACGGAGGTGGAGTGTGGATCGGCAACACGACTTGGTAAATTAGGGATAGAATTTAAGGAAATTATTACAGATAACCTTATGGACGTGTCCTTCAACTTCAAAACAGGAGTGCTGAGTGTGCCACAGCTGAAGCTGGATCACACCACTCTACCAAATTTATTAAATATGATGACATTTGAGAAATTCCATGACCAGATTGGAAGTGAAGTAATTTCATTTGTGGTGTTTATGACAAATCTAATTGAAGCCGAAGAAGATGTGTCGCTGCTAATCTCAAAGAAAATATTAAGGAATGAACTTGATCAAGACCGTAAAGTTGTGTTACAATTGTTCAATCAGCTGTCAAAAGGACCAACCAAATGTTTGGATACACACATTTGTGAAGTATTAAACATGTTGAATAAGCATTGTAATAATCCTTGGACTATGTGGTGGGTAAATCTCAAAGACCCTCGAACTCAAGATCCATGGGTTATTATCTCCGTTGCTGCCGTTCTCTTTGGTTTTCTGCTCCAAATTATCCAAACCGTCTACGGAATTTATGGAATTTACCGCCCAAATCATTAACCACATTGTTTTTTTTCTTTTTTTTTTTTT

Coding sequence (CDS)

ATGAATATGATGCAGTCAACAGTAGGCAGCGAATTCTTTGGTGGAGACGATGATGAGTTTATTATTAAAGATATGGGTAAGGTTGTAAATGAGGTGAAAGAGCTTCTGAATAAAGTTGAGGATAATGTTTTGAAGGAGGAGTTACGTAAGATGCAAGAATTATCAAGGCAAAAGGGGAAAGCTTCAATCTACAAAATACCAGAAGAAATAAGAGAAGTTAATCCCAAAGCTTTCGAGCCACAGTTGGTGTCTTTTGGGCCATACCATTATGGGAAACCCAATTTGCTTGAAATGGAATTGGAAAAACAGAATGGGTATCTCCGTTTTAAAGCTAAAAACAATTTAGAATTTGAGTCCATTATTGTGGAGAGTGTGTCGAGCACTTTGAAACAGCTGTTGGGATCTTATGATAATCTTGAGGATAAATGGAAGCAAAATCCTGCTAAGTTCTTGAAGCTCATGATTGTGGATGGCTGTTTCATGCTGGATTTGATAATCAATGGATGTCCTCAATCGCTAGCTTCTATGATTGGGAATATAAAGCGGGACATGCTGTTGCTTGAGAATCAACTACCCTTCAACCTACTTCAACACCTCTATGATTTAGTGAACAACAACGGCCATAAGGATAAAGGGAGTCTGAGCTTGCTGATTTTGTACTGGATGGGGATACCGAAGGGAGGGGTAATAACGGGAAAATACTTGCACATTTTGGATATGCACAGAGCTTTAATGTTGTATCCTTCAATAGAAAAGACAGATGATTACAATAGTAGAAGCCGAAGAAGGTACAACCAGACGGAGGTGGAGTGTGGATCGGCAACACGACTTGGTAAATTAGGGATAGAATTTAAGGAAATTATTACAGATAACCTTATGGACGTGTCCTTCAACTTCAAAACAGGAGTGCTGAGTGTGCCACAGCTGAAGCTGGATCACACCACTCTACCAAATTTATTAAATATGATGACATTTGAGAAATTCCATGACCAGATTGGAAGTGAAGTAATTTCATTTGTGGTGTTTATGACAAATCTAATTGAAGCCGAAGAAGATGTGTCGCTGCTAATCTCAAAGAAAATATTAAGGAATGAACTTGATCAAGACCGTAAAGTTGTGTTACAATTGTTCAATCAGCTGTCAAAAGGACCAACCAAATGTTTGGATACACACATTTGTGAAGTATTAAACATGTTGAATAAGCATTGTAATAATCCTTGGACTATGTGGTGGGTAAATCTCAAAGACCCTCGAACTCAAGATCCATGGGTTATTATCTCCGTTGCTGCCGTTCTCTTTGGTTTTCTGCTCCAAATTATCCAAACCGTCTACGGAATTTATGGAATTTACCGCCCAAATCATTAA

Protein sequence

MNMMQSTVGSEFFGGDDDEFIIKDMGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSYDNLEDKWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLASMIGNIKRDMLLLENQLPFNLLQHLYDLVNNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDDYNSRSRRRYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLDHTTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVLFGFLLQIIQTVYGIYGIYRPNH
Homology
BLAST of Lsi09G017750 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 5.8e-32
Identity = 122/433 (28.18%), Postives = 205/433 (47.34%), Query Frame = 0

Query: 55  SRQKGKASIYKIPEEIREVNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRF--KAK 114
           S  K    I+++PE    +NPKA++P++VS GPYHYG+ +L  ++  K      F  +AK
Sbjct: 40  SAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAK 99

Query: 115 NNLEFESIIVESVSSTLKQLLGSYDNLEDKWKQNPAKFLK-------LMIVDGCF--MLD 174
                E+++V++V            +LEDK +++ ++ LK       +M++DGCF  M+ 
Sbjct: 100 KKDVEENVLVKAVV-----------DLEDKIRKSYSEELKTGHDLMFMMVLDGCFILMVF 159

Query: 175 LIINGCPQ-------SLASMIGNIKRDMLLLENQLPFNLLQHLY---------DLVNNNG 234
           LI++G  +       S+  ++ +I+ D+LLLENQ+PF +LQ LY         DL     
Sbjct: 160 LIMSGNIELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAF 219

Query: 235 HKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDDYNS-------RS 294
           H  K  +     YW           K  H+LD+ R   L P+  ++D  +S         
Sbjct: 220 HFFKNPIDKEGSYWEKHR-----NYKAKHLLDLIRETFL-PNTSESDKASSPHVQVQLHE 279

Query: 295 RRRYNQTEVECG------SATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLDHT 354
            +  N   V+        SA RL   GI+F+   +     ++   K   L +PQL+ D  
Sbjct: 280 GKSGNVPSVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGF 339

Query: 355 TLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKVVL 414
                LN + FE+F+    +E+ +++VFM  L+  EEDV+ L + K++          V 
Sbjct: 340 ISSFFLNCVAFEQFYTDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVS 399

Query: 415 QLFNQLSKGPTKCLDT-HICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVLF 447
           + F  +SK     +DT ++  V   +N++    +   W   +    + PW  +S  AVLF
Sbjct: 400 EFFKTISKDVVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLF 455

BLAST of Lsi09G017750 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.2e-05
Identity = 41/164 (25.00%), Postives = 80/164 (48.78%), Query Frame = 0

Query: 42  NVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQLVSFGPYHYGKPNLLEMELE 101
           NV K    +++E   ++   SI+ +P+ +   +P ++ P  VS GPYH  KP L EME  
Sbjct: 24  NVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERY 83

Query: 102 KQNGYLRFKAK-NNLEFESII--VESVSSTLKQLLGSYDNLEDKWKQNPAKFLKLMIVDG 161
           K     + + + N+  F  ++  ++S+   ++     Y         N    L +M VD 
Sbjct: 84  KLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGF------NGETLLWIMAVDS 143

Query: 162 CFMLDLIINGCPQSLASMI-----GNIKRDMLLLENQLPFNLLQ 198
            F+++ +     + + ++I       I RD++++ENQ+P  +L+
Sbjct: 144 SFLIEFLKIYSFRKVETLINRVGHNEILRDIMMIENQIPLFVLR 181

BLAST of Lsi09G017750 vs. ExPASy TrEMBL
Match: A0A5A7TA99 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001800 PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 4.1e-97
Identity = 218/434 (50.23%), Postives = 283/434 (65.21%), Query Frame = 0

Query: 25  MGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQLVS 84
           MG VVNEV ELL+K+E+++  EE    Q +     K SIYKIP+ IREVNPKA+EP+LVS
Sbjct: 1   MGGVVNEVNELLSKLEESIGMEEFLSTQHMI----KPSIYKIPQFIREVNPKAYEPKLVS 60

Query: 85  FGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSY-DNLEDKW 144
            GPYHYGKP+LL ME EK   +L FKA+NNL  ES IV+SV S L+ LLGSY DNL+D W
Sbjct: 61  LGPYHYGKPHLLPMEREKYKAFLHFKARNNLYLES-IVDSVCSILEDLLGSYDDNLDDIW 120

Query: 145 KQNPAKFLKLMIVDGCFMLDLIINGCPQSLA-SMIGNIKRDMLLLENQLPFNLLQHLYDL 204
           K++ A FLKLMIVDGCF+LDLI     +SL   MI +IKRDM+LLENQLP  LL+ L+ L
Sbjct: 121 KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL 180

Query: 205 VNNNGHKDKGSLSLLILYWMGIPKGGVITG--KYLHILDMHRALMLYPSIEKTDDYNSR- 264
                  +   L L  + W  +   G I      LHILDM+R+ +L P+I K D+ +   
Sbjct: 181 TTTTTENENEHLDLEAMIWSWMRLSGEILSMRNPLHILDMYRSSLLSPTICKQDETHENM 240

Query: 265 ----SRRRYNQTEVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLD 324
                    N+  VEC    SAT+L K GI+F++  T+NLMDVSF+FK  VL +P L +D
Sbjct: 241 IAILEWTLTNEDNVECQSIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAID 300

Query: 325 H-TTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRK 384
              T P L+N+M FEK H  +  +V SFVV M NLI  ++DVSLL SK IL + +  D  
Sbjct: 301 DILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKDVSLLASKGILSSNVIHDGN 360

Query: 385 VVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVN--LKDPRTQDPWVIISVA 444
            V+QLFN L+KG TK L+ H+CE+  MLN + +N W++W  N  LK P+T++PW IIS+A
Sbjct: 361 GVVQLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEIISIA 420

BLAST of Lsi09G017750 vs. ExPASy TrEMBL
Match: A0A1S4E2L7 (uncharacterized protein LOC103499077 OS=Cucumis melo OX=3656 GN=LOC103499077 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 2.6e-96
Identity = 219/439 (49.89%), Postives = 287/439 (65.38%), Query Frame = 0

Query: 23  KDMGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQL 82
           ++MG VVNEV ELL+K+E+++  EE    Q +     K SIYKIP+ IREVNPKA+EP+L
Sbjct: 4   EEMGGVVNEVNELLSKLEESIGMEEFLSTQHMI----KPSIYKIPQFIREVNPKAYEPKL 63

Query: 83  VSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSY-DNLED 142
           VS GPYHYGKP+LL ME EK   +L FKA+NNL  ES IV+SV S L+ LLGSY DNL+D
Sbjct: 64  VSLGPYHYGKPHLLPMEREKYKAFLHFKARNNLYLES-IVDSVCSILEDLLGSYDDNLDD 123

Query: 143 KWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLA-SMIGNIKRDMLLLENQLPFNLLQHLY 202
            WK++ A FLKLMIVDGCF+LDLI     +SL   MI +IKRDM+LLENQLP  LL+ L+
Sbjct: 124 IWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 183

Query: 203 DLV-----NNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDD 262
            L      N N H D   L  +I  WM +    +     LHILDM+R+ +L P+I K D+
Sbjct: 184 ALTTTTTENENEHLD---LEAMIWSWMRLSGEILSVRNPLHILDMYRSSLLSPTICKQDE 243

Query: 263 YNSR-----SRRRYNQTEVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVP 322
            +            N+  VEC    SAT+L K GI+F++  T+NLMDVSF+FK  VL +P
Sbjct: 244 THENMIAILEWTLTNEDNVECQSIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLP 303

Query: 323 QLKLDH-TTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNEL 382
            L +D   T P L+N+M FEK H  +  +V SFVV M NLI  +++VSLL SK IL + +
Sbjct: 304 SLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKNVSLLASKGILSSNV 363

Query: 383 DQDRKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVN--LKDPRTQDPWV 442
             D   V+QLFN L+KG TK L+ H+CE+  MLN + +N W++W  N  LK P+T++PW 
Sbjct: 364 IHDGNGVVQLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWE 423

Query: 443 IISVAAVLFGFLLQIIQTV 444
            IS+A   FGF   ++Q +
Sbjct: 424 FISIAFFTFGFTYPLVQAI 434

BLAST of Lsi09G017750 vs. ExPASy TrEMBL
Match: A0A0A0KAA3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G091270 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 5.3e-89
Identity = 210/437 (48.05%), Postives = 275/437 (62.93%), Query Frame = 0

Query: 23  KDMGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQL 82
           ++MG VVNEV  LL+K+E+++  EE      LS Q  K SIYKIP+ IREVNPKA+EP+L
Sbjct: 7   EEMGGVVNEVNALLSKLEESIGMEEF-----LSTQHIKPSIYKIPQFIREVNPKAYEPKL 66

Query: 83  VSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSY-DNLED 142
           VS GPYHYGKP+LL ME EK   +L FKA+NNL  ES IV+SV S L+ LLGSY DNL+D
Sbjct: 67  VSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLES-IVDSVCSILEDLLGSYDDNLDD 126

Query: 143 KWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLA-SMIGNIKRDMLLLENQLPFNLLQHLY 202
           +WK++ A FLKLMIVDGCF+LDLI     +SL   MI +IKRDM+LLENQLP  LL+ L+
Sbjct: 127 RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 186

Query: 203 DL-VNNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDD---- 262
            L   N   K+   L  +I  WMG+    +     LHILD++R+ +L P+  K D+    
Sbjct: 187 ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN 246

Query: 263 --------YNSRSRRRYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVP 322
                     +     Y Q+ +   SAT+L K GI+F++  T+NLMDVSF+FK  VL +P
Sbjct: 247 TITILEWTLTNEENVEYCQSNIR--SATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLP 306

Query: 323 QLKLDH-TTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNEL 382
            L +D   T P L+N+M FEK H  +  +V SFVV M NLI  E+DVSLL SK IL + +
Sbjct: 307 SLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNV 366

Query: 383 DQDRKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVII 442
             D   V+QLFN L+KG TK L++HI  + N+               LK P+T++ W II
Sbjct: 367 IHDGNGVVQLFNVLAKGQTKYLESHIIWLENL--------------KLKVPQTKNSWQII 421

Query: 443 SVAAVLFGFLLQIIQTV 444
           S+A   FGF    +Q +
Sbjct: 427 SIALFTFGFTYPFVQAI 421

BLAST of Lsi09G017750 vs. ExPASy TrEMBL
Match: A0A6J1HB25 (UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461183 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 3.7e-82
Identity = 189/441 (42.86%), Postives = 268/441 (60.77%), Query Frame = 0

Query: 15  GDDDEFIIKDMGKVVNEVKELLNKVEDN--VLKEELRKMQELSRQKGKASIYKIPEEIRE 74
           G DD   I D   +V  VK  L+++ D+  +L  E  +  EL       SIYKIP  + +
Sbjct: 12  GRDDN--IGDNADMVVRVKGTLDQLLDSPAILNMEAEQSSEL------LSIYKIPFFMTQ 71

Query: 75  VNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQL 134
            +PKA+EPQ+VS GPY++GK +L  MELEK   +  FKA+  L+ ES IV  VS+ L +L
Sbjct: 72  THPKAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKARCLLDVES-IVRGVSTILDEL 131

Query: 135 LGSYDNLEDKWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLASMIGNIKRDMLLLENQLP 194
           + SYD LE+ WK++P KFL+LMIVDGCFML  +IN CP SL ++  +IK+DMLLLENQLP
Sbjct: 132 MESYDKLEEDWKEDPGKFLQLMIVDGCFMLGFLIN-CPDSLINVSPDIKQDMLLLENQLP 191

Query: 195 FNLLQHLYDLVNNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEK 254
             LL+ LY + + N    +  L  L+  W+ IP+  V+    LHIL+M++  +L+P I++
Sbjct: 192 MLLLEKLYSIADRNVQLPQQDLKKLVSNWLNIPRNEVMK-DCLHILEMYKESLLHPPIDR 251

Query: 255 TDDYNSRSRRRYNQTEVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQL 314
           TD            ++ EC     AT+L + GI+FK   T +L DV F+ K GVL +P+L
Sbjct: 252 TD-----WSVELGHSDPECQVIPPATKLREAGIKFKRSKTGSLTDVFFDAKGGVLWLPRL 311

Query: 315 KLDHTTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQD 374
            +D  T   LLN+M FEK H + G +V SFV+ M+NLI+ E DV++L  +++L N +  D
Sbjct: 312 MVDDNTESTLLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGEQVLANAVGND 371

Query: 375 RKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVA 434
           ++    LFN+L  G    LDTH+  V   +N+HCN PW      LK    Q PW IIS+ 
Sbjct: 372 KEAA-GLFNRLGSGAAMGLDTHMAWVHKKVNEHCNQPWNERCATLKHEYFQSPWTIISLC 431

Query: 435 AVLFGFLLQIIQTVYGIYGIY 451
           A +FGF++ I+Q +Y     Y
Sbjct: 432 AAIFGFIILILQAIYQFLDYY 435

BLAST of Lsi09G017750 vs. ExPASy TrEMBL
Match: A0A6J1KYV8 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497633 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 5.4e-81
Identity = 187/441 (42.40%), Postives = 266/441 (60.32%), Query Frame = 0

Query: 15  GDDDEFIIKDMGKVVNEVKELLNKVEDN--VLKEELRKMQELSRQKGKASIYKIPEEIRE 74
           G DD   I D   +V  VK  L+++ D+  +L  E  +  EL       SIYKIP  + +
Sbjct: 12  GRDDN--IGDKADMVVRVKGTLDQLLDSPAILNMEAEQSSEL------LSIYKIPFFMTQ 71

Query: 75  VNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQL 134
            +PKA+EPQ+VS GPY++GK +L  MELEK   +  FK +  L+ ES IV  VS+ L +L
Sbjct: 72  THPKAYEPQVVSLGPYNHGKQHLSPMELEKLKLFHSFKTRCLLDVES-IVRGVSTILDEL 131

Query: 135 LGSYDNLEDKWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLASMIGNIKRDMLLLENQLP 194
           + SYD LE++W Q+P KFL+LMIVDGCFML  +I+ CP SL ++  +IK+DMLLLENQLP
Sbjct: 132 MESYDRLEEEWTQDPGKFLQLMIVDGCFMLGFLIS-CPNSLINVSPDIKQDMLLLENQLP 191

Query: 195 FNLLQHLYDLVNNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEK 254
             LL+ LY +   N    +     L+  W+ IP+  V+    LHIL+M++  +LYP I++
Sbjct: 192 MLLLEKLYSIAGRNVQLPQQDPKKLVCKWLSIPQNEVMK-DCLHILEMYKESLLYPPIDR 251

Query: 255 TDDYNSRSRRRYNQTEVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQL 314
            D          + ++ EC     AT+L + GI+FK   T++L DV F+ K GVL +PQL
Sbjct: 252 RD-----WSAELDHSDPECQVIPPATKLHEAGIKFKRSKTESLRDVWFDTKRGVLWLPQL 311

Query: 315 KLDHTTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQD 374
            +D  T   +LN+M FEK H + G +V SFV+ M+NLI+ E DV++L  +KIL N +  D
Sbjct: 312 MVDDDTESTMLNVMAFEKLHMKAGRKVTSFVILMSNLIDDERDVAVLAGEKILANAVGND 371

Query: 375 RKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVA 434
           ++    LF++L  G    LD+H+  V  M+N HCN PW      LK    Q PW IIS+ 
Sbjct: 372 KEAA-GLFSRLGSGAAMGLDSHMAGVHKMVNAHCNQPWNERCATLKHDYFQSPWTIISLC 431

Query: 435 AVLFGFLLQIIQTVYGIYGIY 451
           A +FGF++ I+Q +Y     Y
Sbjct: 432 AAIFGFIILILQAIYQFLDYY 435

BLAST of Lsi09G017750 vs. NCBI nr
Match: KAA0039868.1 (UPF0481 protein [Cucumis melo var. makuwa] >TYK24633.1 UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 365.2 bits (936), Expect = 8.4e-97
Identity = 218/434 (50.23%), Postives = 283/434 (65.21%), Query Frame = 0

Query: 25  MGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQLVS 84
           MG VVNEV ELL+K+E+++  EE    Q +     K SIYKIP+ IREVNPKA+EP+LVS
Sbjct: 1   MGGVVNEVNELLSKLEESIGMEEFLSTQHMI----KPSIYKIPQFIREVNPKAYEPKLVS 60

Query: 85  FGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSY-DNLEDKW 144
            GPYHYGKP+LL ME EK   +L FKA+NNL  ES IV+SV S L+ LLGSY DNL+D W
Sbjct: 61  LGPYHYGKPHLLPMEREKYKAFLHFKARNNLYLES-IVDSVCSILEDLLGSYDDNLDDIW 120

Query: 145 KQNPAKFLKLMIVDGCFMLDLIINGCPQSLA-SMIGNIKRDMLLLENQLPFNLLQHLYDL 204
           K++ A FLKLMIVDGCF+LDLI     +SL   MI +IKRDM+LLENQLP  LL+ L+ L
Sbjct: 121 KEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLHAL 180

Query: 205 VNNNGHKDKGSLSLLILYWMGIPKGGVITG--KYLHILDMHRALMLYPSIEKTDDYNSR- 264
                  +   L L  + W  +   G I      LHILDM+R+ +L P+I K D+ +   
Sbjct: 181 TTTTTENENEHLDLEAMIWSWMRLSGEILSMRNPLHILDMYRSSLLSPTICKQDETHENM 240

Query: 265 ----SRRRYNQTEVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLD 324
                    N+  VEC    SAT+L K GI+F++  T+NLMDVSF+FK  VL +P L +D
Sbjct: 241 IAILEWTLTNEDNVECQSIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLPSLAID 300

Query: 325 H-TTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRK 384
              T P L+N+M FEK H  +  +V SFVV M NLI  ++DVSLL SK IL + +  D  
Sbjct: 301 DILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKDVSLLASKGILSSNVIHDGN 360

Query: 385 VVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVN--LKDPRTQDPWVIISVA 444
            V+QLFN L+KG TK L+ H+CE+  MLN + +N W++W  N  LK P+T++PW IIS+A
Sbjct: 361 GVVQLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWEIISIA 420

BLAST of Lsi09G017750 vs. NCBI nr
Match: XP_016902479.1 (PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo])

HSP 1 Score: 362.5 bits (929), Expect = 5.5e-96
Identity = 219/439 (49.89%), Postives = 287/439 (65.38%), Query Frame = 0

Query: 23  KDMGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQL 82
           ++MG VVNEV ELL+K+E+++  EE    Q +     K SIYKIP+ IREVNPKA+EP+L
Sbjct: 4   EEMGGVVNEVNELLSKLEESIGMEEFLSTQHMI----KPSIYKIPQFIREVNPKAYEPKL 63

Query: 83  VSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSY-DNLED 142
           VS GPYHYGKP+LL ME EK   +L FKA+NNL  ES IV+SV S L+ LLGSY DNL+D
Sbjct: 64  VSLGPYHYGKPHLLPMEREKYKAFLHFKARNNLYLES-IVDSVCSILEDLLGSYDDNLDD 123

Query: 143 KWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLA-SMIGNIKRDMLLLENQLPFNLLQHLY 202
            WK++ A FLKLMIVDGCF+LDLI     +SL   MI +IKRDM+LLENQLP  LL+ L+
Sbjct: 124 IWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 183

Query: 203 DLV-----NNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDD 262
            L      N N H D   L  +I  WM +    +     LHILDM+R+ +L P+I K D+
Sbjct: 184 ALTTTTTENENEHLD---LEAMIWSWMRLSGEILSVRNPLHILDMYRSSLLSPTICKQDE 243

Query: 263 YNSR-----SRRRYNQTEVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVP 322
            +            N+  VEC    SAT+L K GI+F++  T+NLMDVSF+FK  VL +P
Sbjct: 244 THENMIAILEWTLTNEDNVECQSIRSATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLP 303

Query: 323 QLKLDH-TTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNEL 382
            L +D   T P L+N+M FEK H  +  +V SFVV M NLI  +++VSLL SK IL + +
Sbjct: 304 SLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGIDKNVSLLASKGILSSNV 363

Query: 383 DQDRKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVN--LKDPRTQDPWV 442
             D   V+QLFN L+KG TK L+ H+CE+  MLN + +N W++W  N  LK P+T++PW 
Sbjct: 364 IHDGNGVVQLFNLLAKGQTKYLENHMCELFKMLNCYYDNRWSIWLENLKLKVPQTKNPWE 423

Query: 443 IISVAAVLFGFLLQIIQTV 444
            IS+A   FGF   ++Q +
Sbjct: 424 FISIAFFTFGFTYPLVQAI 434

BLAST of Lsi09G017750 vs. NCBI nr
Match: XP_004140657.1 (UPF0481 protein At3g47200 [Cucumis sativus] >KAE8646751.1 hypothetical protein Csa_005047 [Cucumis sativus])

HSP 1 Score: 358.2 bits (918), Expect = 1.0e-94
Identity = 216/439 (49.20%), Postives = 285/439 (64.92%), Query Frame = 0

Query: 23  KDMGKVVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQL 82
           ++MG VVNEV  LL+K+E+++  EE      LS Q  K SIYKIP+ IREVNPKA+EP+L
Sbjct: 7   EEMGGVVNEVNALLSKLEESIGMEEF-----LSTQHIKPSIYKIPQFIREVNPKAYEPKL 66

Query: 83  VSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSY-DNLED 142
           VS GPYHYGKP+LL ME EK   +L FKA+NNL  ES IV+SV S L+ LLGSY DNL+D
Sbjct: 67  VSLGPYHYGKPHLLPMEREKHKAFLHFKARNNLYLES-IVDSVCSILEDLLGSYDDNLDD 126

Query: 143 KWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLA-SMIGNIKRDMLLLENQLPFNLLQHLY 202
           +WK++ A FLKLMIVDGCF+LDLI     +SL   MI +IKRDM+LLENQLP  LL+ L+
Sbjct: 127 RWKEDVAMFLKLMIVDGCFVLDLISELSSKSLGRCMIWDIKRDMVLLENQLPLQLLKQLH 186

Query: 203 DL-VNNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDD---- 262
            L   N   K+   L  +I  WMG+    +     LHILD++R+ +L P+  K D+    
Sbjct: 187 ALTTTNENEKENLDLEAMIWSWMGLSGEILSMRSPLHILDIYRSSLLSPTRCKQDETHEN 246

Query: 263 --------YNSRSRRRYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVP 322
                     +     Y Q+ +   SAT+L K GI+F++  T+NLMDVSF+FK  VL +P
Sbjct: 247 TITILEWTLTNEENVEYCQSNIR--SATQLRKAGIKFEKSSTNNLMDVSFDFKQRVLRLP 306

Query: 323 QLKLDH-TTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNEL 382
            L +D   T P L+N+M FEK H  +  +V SFVV M NLI  E+DVSLL SK IL + +
Sbjct: 307 SLAIDDILTEPTLINIMAFEKLHVGVERQVTSFVVLMKNLIGMEKDVSLLASKGILSSNV 366

Query: 383 DQDRKVVLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVN--LKDPRTQDPWV 442
             D   V+QLFN L+KG TK L++H+ E+  MLN + +N W++W  N  LK P+T++ W 
Sbjct: 367 IHDGNGVVQLFNVLAKGQTKYLESHMYELFRMLNCYHDNRWSIWLENLKLKVPQTKNSWQ 426

Query: 443 IISVAAVLFGFLLQIIQTV 444
           IIS+A   FGF    +Q +
Sbjct: 427 IISIALFTFGFTYPFVQAI 437

BLAST of Lsi09G017750 vs. NCBI nr
Match: XP_038875622.1 (UPF0481 protein At3g47200-like [Benincasa hispida])

HSP 1 Score: 355.5 bits (911), Expect = 6.7e-94
Identity = 207/429 (48.25%), Postives = 277/429 (64.57%), Query Frame = 0

Query: 28  VVNEVKELLNKVEDNVLKEELRKMQELSRQKGKASIYKIPEEIREVNPKAFEPQLVSFGP 87
           VV +VK  LNK+ D+   +E+  + E +    + SIYKIPE +R++  KAFEPQLVS GP
Sbjct: 24  VVIQVKNNLNKLLDSAAVDEMGPLGEST----QPSIYKIPEFMRKIQAKAFEPQLVSLGP 83

Query: 88  YHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSYDNLEDKWKQNP 147
           YH+GK +L+ ME EKQ  + RF     L  ES IVES+S+ L+ L G+YD L++KWKQ+ 
Sbjct: 84  YHHGKEHLVSMEEEKQKAFRRFVNDYGLNLES-IVESMSNNLENLWGAYDKLDEKWKQDG 143

Query: 148 AKFLKLMIVDGCFMLDLIINGCPQSLASMIGNIKRDMLLLENQLPFNLLQHLYDLVNNNG 207
           AKFL++MIVDGCFMLD     CP SL++M  +IKRDMLLLENQLP  LLQ LYD + N  
Sbjct: 144 AKFLEVMIVDGCFMLD-FFKECPYSLSTMRWDIKRDMLLLENQLPMQLLQELYDNMPNTN 203

Query: 208 HKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDDYNSRSRRRYNQT 267
            +D+ SL  LI   M I +  V+ G+ LHILDM+RA +LYP +++  D + +++  Y Q+
Sbjct: 204 QEDQ-SLRSLICQPMCINEEAVM-GRELHILDMYRASLLYPPVDR-KDRSGKTKVTYQQS 263

Query: 268 EVEC---GSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLDHTTLPNLLNMMT 327
           + EC     AT+L   GI+FK   T NL DVSFN K GVL +P + +D  T   LLN+M 
Sbjct: 264 DPECQIIPQATQLHDAGIKFKSSPTKNLTDVSFNPKQGVLELPYIVVDDDTETTLLNVMA 323

Query: 328 FEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKVVLQLFNQLSKGP 387
           FEK + + GS+V SFV+ M NLI+ + DV+LL S KIL N L  D      LF+ L KG 
Sbjct: 324 FEKLYVEAGSKVTSFVILMNNLIDVDRDVALLASDKILANALGNDESAA-DLFSLLGKGA 383

Query: 388 TKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVLFGFLLQIIQTVY 447
              LD+HI +V   +NKHC+  W  W  +LK    Q+PW IIS+ A +FGF + I+Q +Y
Sbjct: 384 AMDLDSHITDVHYKVNKHCSLSWNQWCASLKHDYFQNPWAIISLFAAVFGFAILIVQAIY 442

Query: 448 GIYGIYRPN 454
            I   +R N
Sbjct: 444 QIVDYHRGN 442

BLAST of Lsi09G017750 vs. NCBI nr
Match: XP_023513986.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 316.2 bits (809), Expect = 4.5e-82
Identity = 188/438 (42.92%), Postives = 270/438 (61.64%), Query Frame = 0

Query: 15  GDDDEFIIKDMGKVVNEVKELLNKVEDN--VLKEELRKMQELSRQKGKASIYKIPEEIRE 74
           G DD   I D   +V +VK  L+++ D+  +L  E  +  EL       SIYKIP  + +
Sbjct: 12  GRDDN--IGDKADMVVQVKGTLDQLLDSPAILNMEAEQSSEL------LSIYKIPFFMTQ 71

Query: 75  VNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQL 134
            +PKA+EP++VS GPY++GK +L  MELEK   +  FK +  L+ ES IV+ VS+ L +L
Sbjct: 72  THPKAYEPRVVSLGPYNHGKQHLSPMELEKLKLFYSFKTRCLLDVES-IVKGVSTILDEL 131

Query: 135 LGSYDNLEDKWKQNPAKFLKLMIVDGCFMLDLIINGCPQSLASMIGNIKRDMLLLENQLP 194
           + SYD LE++W ++P KFL+LMIVDGCFML  +IN CP SL ++  +IK+DMLLLENQLP
Sbjct: 132 MESYDKLEEEWTEDPGKFLQLMIVDGCFMLGFLIN-CPDSLINVSPDIKQDMLLLENQLP 191

Query: 195 FNLLQHLYDLVNNNGHKDKGSLSLLILYWMGIPKGGVITGKYLHILDMHRALMLYPSIEK 254
             LL+ LY +   N    +  L  L+  W+ IP+  V+    LHIL+M++  +L+P I++
Sbjct: 192 MLLLEKLYSIAARNVQLPQQDLQKLVSNWLNIPRNEVMK-DCLHILEMYKESLLHPPIDR 251

Query: 255 TDDYNSRSRRRYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLD 314
           T D++        +++V    AT+L + GI+FK   TD+L DV F+ K GVL +PQL +D
Sbjct: 252 T-DWSVELDHSDPESQV-IPPATKLREAGIKFKRSKTDSLTDVFFDAKGGVLWLPQLMVD 311

Query: 315 HTTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKV 374
             T   LLN+M FEK H + G  V SFV+ M+NLI+ E DV++L  +K+L N +  D++ 
Sbjct: 312 DDTESTLLNVMAFEKLHMKAGRRVTSFVILMSNLIDDERDVAVLAGEKVLANAVGNDKEA 371

Query: 375 VLQLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVL 434
              LFN+L  G    LDTH+  V   +N+HCN PW      LK    Q PW IIS+ A +
Sbjct: 372 A-GLFNRLGSGAAMGLDTHMAGVHKKVNEHCNQPWNERCATLKHDYFQSPWTIISLCAAI 431

Query: 435 FGFLLQIIQTVYGIYGIY 451
           FGF++ I+Q +Y     Y
Sbjct: 432 FGFIILILQAIYQFLDYY 435

BLAST of Lsi09G017750 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 155.6 bits (392), Expect = 9.5e-38
Identity = 125/449 (27.84%), Postives = 210/449 (46.77%), Query Frame = 0

Query: 59  GKASIYKIPEEIREVNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFE 118
           GK  IY++P  ++E + K++ PQ VS GPYH+GK  L  ME  K      ++A N     
Sbjct: 111 GKLCIYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRPMERHK------WRALN----- 170

Query: 119 SIIVESVSSTLKQLLGSYDN----LEDKWK---QNPA-----KFLKLMIVDGCFMLDLI- 178
                 V   LKQ +  Y N    LE+K +   + P      +F +++++DGCF+L+L  
Sbjct: 171 -----KVLKRLKQRIEMYTNAMRELEEKARACYEGPISLSRNEFTEMLVLDGCFVLELFR 230

Query: 179 --INGCPQ----------SLASMIGNIKRDMLLLENQLPFNLLQHLYDLVNNNGHKDKGS 238
             + G  +          ++  ++ +I+RDM++LENQLP  +L  L +L     ++    
Sbjct: 231 GTVEGFTEIGYARNDPVFAMRGLMHSIQRDMIMLENQLPLFVLDRLLELQLGTQNQTGIV 290

Query: 239 LSLLILYWMGI-PKGGVITGK--------------------YLHILDMHRALMLYP---- 298
             + + ++  + P G  +T                       LH LD+ R  +L      
Sbjct: 291 AHVAVKFFDPLMPTGEALTKPDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQSSPTP 350

Query: 299 ---SIEKTDDYNSRSRRRYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLS 358
              S+ K    N+R   +  Q  V C   T L + G++F++  TD   D+   FK G L 
Sbjct: 351 NTRSLLKRLTRNTRVVDKRQQQLVHC--VTELREAGVKFRKRKTDRFWDI--EFKNGYLE 410

Query: 359 VPQLKLDHTTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNE 418
           +P+L +   T     N++ FE+ H +  + + S+++FM NLI + EDVS L    I+ + 
Sbjct: 411 IPKLLIHDGTKSLFSNLIAFEQCHIESSNHITSYIIFMDNLINSSEDVSYLHYCGIIEHW 470

Query: 419 LDQDRKVVLQLFNQLSKGPT-KCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWV 454
           L  D +V   LFN+L +       D+H+  +   +N++ N  W +    L      +PW 
Sbjct: 471 LGSDSEVA-DLFNRLCQEVVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWA 530

BLAST of Lsi09G017750 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 154.8 bits (390), Expect = 1.6e-37
Identity = 117/438 (26.71%), Postives = 206/438 (47.03%), Query Frame = 0

Query: 59  GKASIYKIPEEIREVNPKAFEPQLVSFGPYHYGKPNLLEMELEKQNGYLRFKAKNNLEFE 118
           GK  IY++P  ++E + K++ PQ VS GPYH+GK  L  M+  K     R   + N   +
Sbjct: 101 GKLCIYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIK 160

Query: 119 SIIVESVSSTLKQLLGSYDNLEDKWKQNPAKFLKLMIVDGCFMLDLI---INGCPQ---- 178
            + ++++    ++    Y   E     +  +F++++++DGCF+L+L    + G  +    
Sbjct: 161 -MYIDAMRELEEKARACY---EGPLSLSSNEFIEMLVLDGCFVLELFRGAVEGFTELGYA 220

Query: 179 ------SLASMIGNIKRDMLLLENQLPFNLLQHLYDLVNNNGHKDKGSLSLLILYWMGI- 238
                 ++   + +I+RDM++LENQLP  +L  L +L     ++      L I ++  + 
Sbjct: 221 RNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLELQLGTRNQTGLVAQLAIRFFDPLM 280

Query: 239 PKGGVITGK----------------------YLHILDMHRALMLYPS-------IEKTDD 298
           P    +T                         LH LD+ R  +L  S         K   
Sbjct: 281 PTDEPLTKSGQSKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRKRWS 340

Query: 299 YNSRSRRRYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLDHTT 358
            N+R   +  Q  + C   T L + GI+F+   TD   D+   FK G L +P+L +   T
Sbjct: 341 RNTRVADKRRQQLIHC--VTELKEAGIKFRRRKTDRFWDM--QFKNGYLEIPRLLIHDGT 400

Query: 359 LPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKVVLQ 418
               LN++ FE+ H    +++ S+++FM NLI++ EDVS L    I+ + L  D +V   
Sbjct: 401 KSLFLNLIAFEQCHIDSSNDITSYIIFMDNLIDSHEDVSYLHYCGIIEHWLGSDSEVA-D 460

Query: 419 LFNQLSKGPT-KCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVLFG 453
           LFN+L +       D+++  +   +N++ ++ W  W   LK     +PW I+S  A +  
Sbjct: 461 LFNRLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAAVIL 520

BLAST of Lsi09G017750 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 147.5 bits (371), Expect = 2.6e-35
Identity = 116/431 (26.91%), Postives = 202/431 (46.87%), Query Frame = 0

Query: 60  KASIYKIPEEIREVNPKAFEPQLVSFGPYHYGKPNLLEMELEKQN--GYLRFKAKNNLEF 119
           K  IY++P  ++E + K++ PQ VS GPYH+GK +L  ME  K      +  + K+N+E 
Sbjct: 89  KLCIYRVPFYLQENDKKSYLPQTVSIGPYHHGKVHLRPMERHKWRAVNMIMARTKHNIE- 148

Query: 120 ESIIVESVSSTLKQLLGSYDNLEDKWKQNPAKFLKLMIVDGCFMLDLI---INGCPQ--- 179
             + ++++    ++    Y    D   +N  +F +++++DGCF+L+L    I G  +   
Sbjct: 149 --MYIDAMKELEEEARACYQGPID--MKNSNEFTEMLVLDGCFVLELFKGTIQGFQKIGY 208

Query: 180 -------SLASMIGNIKRDMLLLENQLPFNLLQHLYDLVNNNGHKDKGSLSLLILYWMGI 239
                  +   ++ +I+RDM++LENQLP  +L  L  L     ++      + + ++  +
Sbjct: 209 ARNDPVFAKRGLMHSIQRDMIMLENQLPLFVLDRLLGLQTGTPNQTGIVAEVAVRFFKTL 268

Query: 240 -PKGGVITGKY-----------------LHILDM-HRALMLYPSIEKTDD---YNSRSRR 299
            P   V+T                    LH LD+ HR+L+   S E T+    Y   S  
Sbjct: 269 MPTSEVLTKSERSLDSQEKSDELGDNGGLHCLDVFHRSLI--QSSETTNQGTPYEDMSMV 328

Query: 300 RYNQTEVECGSATRLGKLGIEFKEIITDNLMDVSFNFKTGVLSVPQLKLDHTTLPNLLNM 359
              Q  + C   T L   G+ F    T  L D+   FK G L +P+L +   T     N+
Sbjct: 329 EKQQQLIHC--VTELRGAGVNFMRKETGQLWDI--EFKNGYLKIPKLLIHDGTKSLFSNL 388

Query: 360 MTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKVVLQLFNQLSK 419
           + FE+ H Q  + + S+++FM NLI + +DVS L    I+ + L  D +V   LFN+L K
Sbjct: 389 IAFEQCHTQSSNNITSYIIFMDNLINSSQDVSYLHHDGIIEHWLGSDSEVA-DLFNRLCK 448

Query: 420 GPT-KCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVLFGFLLQIIQ 453
                  D ++ ++   +N++ +  W      L+     +PW   S +A +    L   Q
Sbjct: 449 EVIFDPKDGYLSQLSREVNRYYSRKWNSLKATLRQKYFNNPWAYFSFSAAVILLFLTFFQ 507

BLAST of Lsi09G017750 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 144.4 bits (363), Expect = 2.2e-34
Identity = 122/435 (28.05%), Postives = 208/435 (47.82%), Query Frame = 0

Query: 34  ELLNKVEDNVLKEELR-KMQELSRQKGKASIYKIPEEIREVNPKAFEPQLVSFGPYHYGK 93
           E +N+ E + L + ++ K+  LS    K  IYK+P ++R +NP A+ P+LVSFGP H GK
Sbjct: 265 ERMNQNEGDALVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGK 324

Query: 94  PNLLEMELEKQNGYLRFKAKNNLEFESIIVESVSSTLKQLLGSYDNLEDKWKQNPAKFLK 153
             L  ME +K    L F  + N   E ++   ++ T +Q   +     +  K +  +F++
Sbjct: 325 EELQAMEDQKYRYLLSFIPRTNSSLEDLV--RLARTWEQ--NARSCYAEDVKLHSDEFVE 384

Query: 154 LMIVDGCFMLDLIINGCPQSL----------ASMIGNIKRDMLLLENQLPFNLLQHLYDL 213
           +++VDG F+++L++      L          + MI ++ RDM+L+ENQLPF +++ ++ L
Sbjct: 385 MLVVDGSFLVELLLRSHYPRLRGENDRIFGNSMMITDVCRDMILIENQLPFFVVKEIFLL 444

Query: 214 VNNNGHKDKGSLSLLIL------YWMGIPKGGVITGKYLHILDMHRALMLYPSIEKTDDY 273
           + N  +  +G+ S++ L      Y++          +  H +D+ R+  L     K +  
Sbjct: 445 LLN--YYQQGTPSIIQLAQRHFSYFLSRIDDEKFITEPEHFVDLLRSCYLPQFPIKLE-- 504

Query: 274 NSRSRRRYNQTEVE-CGSATRLGKLGIEFKEIITDN-LMDVSFNFKTGVLSVPQLKLDHT 333
                  Y   +V+    AT L   G+ FK   T + L+D+S  F  GVL +P + +D  
Sbjct: 505 -------YTTVKVDNAPEATELHTAGVRFKPAETSSCLLDIS--FADGVLKIPTIVVDDL 564

Query: 334 TLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEEDVSLLISKKILRNELDQDRKVVL 393
           T     N++ FE+         + +++ +   I++  D  LLI   I+ N L      V 
Sbjct: 565 TESLYKNIIGFEQCRCS-NKNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNSVD-VS 624

Query: 394 QLFNQLSKGPTKCLDTHICEVLNMLNKHCNNPWTMWWVNLKDPRTQDPWVIISVAAVLFG 450
            LFN +SK        +   +   L  +CN PW  W   L+     +PW + SV A L  
Sbjct: 625 NLFNSISKEVIYDRRFYFSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLL 680

BLAST of Lsi09G017750 vs. TAIR 10
Match: AT3G47250.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-34
Identity = 125/462 (27.06%), Postives = 226/462 (48.92%), Query Frame = 0

Query: 35  LLNKVEDNVLKEELRKMQE------LSRQKGKAS--IYKIPEEIREVNPKAFEPQLVSFG 94
           L  + E +V+    R +Q       L    GKAS  I++IP+ + EVNPKA++P++VS G
Sbjct: 27  LRQRSERSVVSHIKRNLQSSGKPLILLESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIG 86

Query: 95  PYHYGKPNLLEMELEKQNGYLRF---KAKNNLEFESIIVESVSSTLKQLLGSYDNLEDKW 154
           PYHYG+ N L+M  + +  +L     +A      E+++  +V +   ++  SY    ++ 
Sbjct: 87  PYHYGE-NHLQMIQQHKFRFLELFVDRATKKGMDENVLYAAVGALQHKIRASY---SEEL 146

Query: 155 KQNPAKFLKLMIVDGCFML----------DLIINGCP-QSLASMIGNIKRDMLLLENQLP 214
           +   ++ + +MI+DGCF+L          DL +N  P  ++  ++ +I+ D+LLLENQ+P
Sbjct: 147 RIEKSELVSMMILDGCFILMLLLIVSRKIDLDMNKDPIFTIPWILASIQSDLLLLENQVP 206

Query: 215 FNLLQHLYDLVNNNGHKDKGSLSLLI--LYWMGIPKGGVITGKY-----LHILDMHRALM 274
           F +L+ ++D    +G    G L+ +    + + I K      K+      H+LD++R   
Sbjct: 207 FFVLRTIFD---KSGIGSPGDLNRMAFSFFNLSIDKPDTYWAKHRDRGAKHLLDLNRKTF 266

Query: 275 LYPSIEKTDDYNSRSRRRYNQTEVECG-------------SATRLGKLGIEFKEIITDNL 334
           + PS+    +  +    ++  ++ + G             SA RL   GI+F+ + +D  
Sbjct: 267 I-PSMRSMGESETTPSSKFQHSKGKSGEVSSSESTFPLILSAKRLRLQGIKFR-LRSDAE 326

Query: 335 MDVSFNFKTGVLSVPQLKLDHTTLPNLLNMMTFEKFHDQIGSEVISFVVFMTNLIEAEED 394
             +    K   L +P L+LD       LN + FE+F+ +  +++ S+VVFM  L+  +ED
Sbjct: 327 SILDIKLKKNKLQIPLLRLDGFISSIFLNCVAFEQFYTESTNDITSYVVFMGCLLNDQED 386

Query: 395 VSLLIS-KKILRNELDQDRKVVLQLFNQLSKGPTKCLDT---HICEVLNMLNKHCNNPWT 451
            + L + K+I+ N    + + V Q F  + K      DT   ++  V   +N++ +  + 
Sbjct: 387 ATFLNNDKRIIENYFGNENE-VSQFFKTICKDVV--FDTRRSYLRNVFEGVNEYTSKTYN 446

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD535.8e-3228.18UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8971.2e-0525.00Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TA994.1e-9750.23UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G0018... [more]
A0A1S4E2L72.6e-9649.89uncharacterized protein LOC103499077 OS=Cucumis melo OX=3656 GN=LOC103499077 PE=... [more]
A0A0A0KAA35.3e-8948.05Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G091270 PE=4 SV=1[more]
A0A6J1HB253.7e-8242.86UPF0481 protein At3g47200-like OS=Cucurbita moschata OX=3662 GN=LOC111461183 PE=... [more]
A0A6J1KYV85.4e-8142.40UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
KAA0039868.18.4e-9750.23UPF0481 protein [Cucumis melo var. makuwa] >TYK24633.1 UPF0481 protein [Cucumis ... [more]
XP_016902479.15.5e-9649.89PREDICTED: uncharacterized protein LOC103499077 [Cucumis melo][more]
XP_004140657.11.0e-9449.20UPF0481 protein At3g47200 [Cucumis sativus] >KAE8646751.1 hypothetical protein C... [more]
XP_038875622.16.7e-9448.25UPF0481 protein At3g47200-like [Benincasa hispida][more]
XP_023513986.14.5e-8242.92UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G50170.19.5e-3827.84Plant protein of unknown function (DUF247) [more]
AT3G50120.11.6e-3726.71Plant protein of unknown function (DUF247) [more]
AT3G50150.12.6e-3526.91Plant protein of unknown function (DUF247) [more]
AT4G31980.12.2e-3428.05unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G47250.16.3e-3427.06Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 22..57
NoneNo IPR availablePANTHERPTHR31549:SF210PROTEIN, PUTATIVE (DUF247)-RELATEDcoord: 49..448
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 63..436
e-value: 4.6E-81
score: 273.1
IPR004158Protein of unknown function DUF247, plantPANTHERPTHR31549PROTEIN, PUTATIVE (DUF247)-RELATED-RELATEDcoord: 49..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G017750.1Lsi09G017750.1mRNA