Lsi09G016990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G016990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncarotenoid cleavage dioxygenase 7, chloroplastic
Locationchr09: 25505283 .. 25512032 (+)
RNA-Seq ExpressionLsi09G016990
SyntenyLsi09G016990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATCGATTTCCACTCCAAACACTGCCCGAATATCGGTCGACAACGACGATGATTCTGTTGCAGCCTTCTGGGACTACCAATTCCTCTTCGTTTCACAGCGTACCGAGACAGTAGACCCCACCGTCCTCCGCCTAGTCGACGGTGCCATACCGCCAAATTTCCCTTCCGGCACGTATTACCTGACGGGACCAGGCATGTTCTCCGACGACCATGGGTCTACGGTGCACCCACTCGACGGCCATGGGTATTTAAGAGCCTTTGTTTTCGAGAAGAAGAAGAAGGAGGTGATTTTCATGGCGAAGTACGTGAAAACAGAGGCGAAAATGGAGGAGCATGATCCGATGACTGACACGTGGCAGTTTACGCATAGAGGACCGTTTTCGGTGCTGAAGGGAGGGAAGAAATTGGGGAATACAAAGGTGATGAAAAATGTGGCTAATACGAGTGTGCTGCGGTGGGGCAGCCGCCTTCTTTGCCTTTGGGAGGGCGGCGATCCGTACGAAATTCAGCCGGAAAATTTGGATACAGTTGGGAAGTTTTCTGTTGTTCAGGGTGGCTGTGATTACGATTTGCCGTCCCCCGGCGGACCTTACGATGCTGGGTTTTGGCGTTTTGCTGCAGACATTTTGAAGCCTGTGCTCTATGGTTGGTATTAATACTTTTAATTATTTTATTTCTTTTCACATTTTTTTTTATATAATATGTGATATGGGAGAATCAAACCTCTATTATTGTGATTGATAGTATAAACAAATATACCAATTGAGCTATCAGGGCAGAACCTCTCTCAATAAGATATGGTTCAAAGACAAACCAAGCGGAAAGTTGGTATGGATATGTGATGCTCGAGTTTAAGAATAGTACATGAATGAACCGATATTATATCTAAATGAGAGGAATTGTGAGGATATGACGGTATTCATTTTGTCATATTTAAAAACCGTCTTTATTAAAACAGTGTCGTTATTTTATTTAATAAAGTGTCCTAAAAAGCAAAGGAATTTTGCTGTAATACCAGACAGATGCCGACACCACAGTAAGAAACAAAAATATAAGACATATAATACATACATAATCATTGGTTTGAATATTTAAAAAATGAAAATAATTTTGGTGGAGTTGGCAATTGGAAATTGAATAATAATATTAAAATATTAAAGTAATTAAAAATTAGAAGGTGGGTGATAAATAAATAAATTAATTAATTAATGGGGGAATAATAATGGGCACAGGAATATTCAAGATGCCACCAAAGAGGTTGTTGTCTCATTACAAGATTGATTCTCAAAGGAAAAGACTTCTCATAATGTCATGCAATGCTGAAGATATGCTTCTACCAACTAGTCATTTCACATTTTATGGTAATTTTTCTTTTTTTTATCTTATTTCTTTTAATTCCTTATTTATTATTATTCATTATTAACAACTCTATATATTCTTTTAAAATTCCCAATTTAATTTCTTTTATTTTACTTTTTGCATTTATCATTACTGACATCAAACTTGCATGATGTCATCTCGGTTAAGCTGCAAAATTAATCGACTATTTATGAAAATTTAATCAAATTATAATAACTATTTATGAAGTTTTATCAATACCAACTTATAAAGACTAAATTTCTTATGACATAATTTGGAGACTTGTCATGATTATTTTAAAGTCATTTTTGAACCATCAATCAATTAAATGTTATCTTTAGAAAAAAATTAAATAGTTTTTTTTTTGTAAGTGTTTCTTATTTAAAAGTTTTTGGTTTTCTATTTTTTTTTTAACTTTATGTTTATTTTCTTTTAAATTTTTTTATTATATTGTCACATTTATTAAACGAAGATTTAAATTATTTAGTAAAATTTCAAAACCTAAAAAAAGTTGTCAAAATTTTTTTTTTATACCCTTTAACACTTGACTTAGTATTTGAAAACATGGTAGAAAGTTATAAATGCTTATAAACTTGAATTTCAAAAACCAAACACTAAAAATTATCATATGGAGCATAATATTTTAAAAAATGTGCTTATAGAAAAATCAAATTTATTTTAAAAATAGGGGAGAACTTTTAAAAATAGCAAAATTTTTAGATAGTTGTGATAGACGCCGATAGAAGTCTATCAGAGTCTATCAGTGATAGAAATAATAGAAGTCTATCGCTGATAGGTGCTGATAGAAGTCTATCACTGATAGGCGCTGATAGAAGTCCATCACTATTTTTTTTTTTTTTTTTTTTTGCTATTTTCTGTAAACCAAAAGTATTCCAACACATTATAAAATAATGTATAATTTCTCTAATATATATTTATAAAACATCATTCTTCTTTTTTTAGTTGTATCGTGGTAATACTCTTCGAAACTTTTATCAACTCGTATAAATTTATCATCAACTAATTAAAACATTACAGACAAAAGAGTATAATTGAAAATGCTAAAAACGCAAAGGACTAAATTGAAACTTTTGGTTTTATTATTTATTACCCTAATTTTCTTTTTGTGTGCAGAATTTGACAGTAATTTCAAGTTATTACAGAATAAAGATTTGGTTATCAATGATCACTTGATGATCCATGATTGGGCTTTTACTGATACTCATTACATCCTCTTTGCTAACCGAATTAAGCTCGATGTCATCGGTATGTTTTTATTTACTTATTTATTTTGAGAATATAATAATATAAACGTAAATATAAAAAATAAAAAAAAATCTAATTTTCAACTTTATAAGAACAATATACCATTTTGCAATTTTTTTAAAAAGTTAAACCTAAAATATTCCTGCACAATTTTTTTTATAAAAATTCTTAAATACTCCAAAGGTATAATAACTTTTTTTTTTTTTTTTTAATTAGAGTTGTTTTCTCATAAATTTTGATACGCTTGTCACTTTTAAAAACAAACTTTTAAATTCCTAGCCTAAATATTTTTTTTAAAAAATAAAGATTTTAAAAACTTTTATTTTTATATTTAAAATTTGGCTTGACTTTTAAAAATATCTTTAAGAATTATGTAATATAGGATGGAAAACTATAAATGGAAGTAATGTTAACAAACTTAATTTTCTAAAACAAAGAAAACTAAAGAGCAAAGGCCTCATTTGATAACTATTTACCCCCCTTACATAACTATTTGATTTTTTGTTTCTTATTTTTAAAATTCATTAAGCATATTTCCTCTCAATTTTTTACAACGACTTTCATTTTTCTTAAGTAAAAGAGTAAAAAACAAAAACAACTTTTTTTAGTTTTCAAAACTTATATTGATTTTTCAAAAACATTGGTAGAAAGTAGATAACAAAAAAAAAAAAAAAAAAAAAAAAAATAGAAGTGTAAGAGGTGTTTATAGACTTAATTTTCAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAGTTACCAAACGAAACCCTAATTTTTTTTTTTTTCATTTTTCGTCTTTGAAAATTAAGCTTATAAATACTAATTCTACTTATTGATTCATTAGTTTTGTTGTCTACTTTTTAGAGATGTTTTAAAAGACAAAGCCAAGTATTGAAACCTTAAAAAAAAAAAAACTTTTTAAAAAAACTTCTTTCTATTTTAGAAATTTGGCTAAAAATTCAAGGGTTTAGTTAGGAAAAATAAAAACCATAATAAAAAAGTTGTAACAAAACAAGCATAATTTTTAAAAACCAGATGATTATCAAACAGAATCAAAATGATTATCAAACCGACCTTTGATTACTTATACCTAGTTGAAAACCTTCTTCTTTTCTCAATTTACATATAAGTGATGATATACTCATGCATTGCTATACTAATCATATTCATATGAATAGAAGATCAAAGTTAAAAAAAAAGATTAACAAAAACTTATTTCAATTAATTGTTGAGTGAAATTAATTAGAACCCAAATTACATAAATTGAAGGTGCAATGGGTGCTGTTAGTGGGATATCTCCAATGATATCAGCACTAAGTGTAAACACAAATAAACCCACTTCTCCAATTTATTTGATTCCAAGATTTGGTGAAGATTCAAACAAAGATTGGAGAGAAGCCATTGTTGAAGTTCCTTCAAGGCTTTGGCTACTTCATGTTGGCAATGCCTTTGAGGTTATTCATGAAGATGGCAACTTGGACTTCCAAATCCATGCCTCTGCTTGCTCCTATCAATGGTTCAATTTTAAAAAATTGTTTGGTAAGTCATTTTTTTTAATTCCCTCTCGTATGCCCTCACCCTTCTTCAGATTTGGTTATTAGATAGATATGAAGTTTCATCTTAAAATTAATTGATAATAAGAGGAATACGACTTATATAGAATCTTTAACCTTCCTTCTCAAGATGAACTCTCTTTGAATTCATCATTCTTGGGTTGGTTTTTGATTTTTTTTTTGAATGAATAATACTTGTTTGAGCTTGATGGACTCTGATACTGTATTAGATAAACATCAAGATTAATTTAGTTGTCTATTTTTCAGGTTATAATTGGCAGACTGGAAAACTTGACCCTTCTATTATGAATATGAATGAATCTAAAAGTGAGCTATTGCCTCACCTAGTTAAGGTAATTTTTTTTTCTAACTAATGTATTTAATGTAACCAAAAAGTTCATTGATTTTATAAATGACAAAGAGTTTTTTTTTTTATTTGGATGAAAATGTTTGATCAAATTTTTAAAAATGGGTAGAAAAACATGTTTAACCCATCGTACTCTTTTAAGCAGTGCTTGAGGCAAGAAGTGTAATTGTTTGATAGTAGTTGTACAGGATAAAGAGTTAATAAATCATGAAATTGATGTTTTTAGTCGTGGAGACTGAACAATAGATTTAAGAACTTCTTAGACCAAACAAAGAGTGAAGTCGTTTAACAGTACTCCTCTCAATTCTTATTCCCCTTAGGCCAAAATATGCCTAATCTAATATACATGAAAACTTTATTAGATACGTAAAGATCATAGACAAACTTCTATAATTCAAAGCATTTAATTAGTCTTATATGAAAATTTTGTTAGATACGTAAAGATCATAGACAAACTTCTATAATTCAAAGCATTTAAGTAGTCTTATTTGTTTTTATATATATATATATATATATATATGATACTTGAGTTGTTTTTTTCATTACCAGTTAATTTTGAGATAACATCCTATGTTTAACTAATAAAATACATCCAAAAATACTGTAGTTATCAATTTAAGCCTAATTCAATTATATAAATTATACTAAAATTAGAAGGATTATTTTAAAATATAGAAAAATGAATCAAACTATTTACAAATATAAAAAAATTTAACTTTCTATTAGTGATAGATCGCTATAGACTTCTATCGCTCAAGGGATAGAAGGGAATAGAAGTCTATTGTGTTCTATCGCTAATAGATAGTGAAATTTTTCTATATTTATAAATGGTTTGATATTTTTTCTATTTGTAGTAATTTTCCAAATTAGAATAACACCTACTTAATTTTCAAAACTTTGAATAATATTTTAATGAAAAAAAAACTAATGAGAAATACATTCAAAATTAACCATTTGCAGGTTTCCATCAGTTTAAGTAAGAATGGGAAGTGTGAGAAGTGCAGTGTGGAGCCTTTGAACCAATGGACAAAAGCCTCAGATTTTCCAGTGATAAACCCTACATTCTCAGGGCTAAAACACAATTACTTATACGCTGCAACTTCCTCCGGTAATCGGCGATCGCTACCGTCGTTTCCTTTCGATATGATCGTAAAGCTCGATACTGTCACCAACACTGTCCGAACATGGTCTGCTGGTAATCGAAGATTCGTCGGAGAACCGGTTTTTGTTCCCAAAGGAGATGAAGAAGACGATGGGTATCTTCTTGTGGTTGAGGTTAGCAAACTTATAAACCATCTCTATTTATCGTCTTTTTTGTGTTTTACTATTAATTCATAATATGCAAATTTTAAGAGTAAAATTGCAATATTTTGAAACTTAAGGACTAAATAGAAATCAAATTCAAAATTTAAGAACTAAAAATATAATATTTTGAAACTTAAGGACCAAATGGAGACTAAACCCAAAATCTAGAGACCAAAAATGTAATTTTCCTTAAAAAAATGTATAGAACACTTTTAAAGTTTAGGGACCTGTAGCATACAAAAATGATGCATTGTTTGATAACTATTTAGTTTTTGAAAATTAAACTTATAATCATTACTTTTCATAAGGTTATTCTCAAACAAGTTTTATCAAAAGAGCTTAAATAAAAATAACTTTTCTGAAAAATATTTTTTCTTTTAGTCAATCCAAACCGACTCTATATTTCTAAATTCCAACTATACCCTTCAAAATGCTATTTATTAATGAAGTAAGAAAATGCAAATAGTTGATGTTATATGGAGGCTAGCATGATTTAAGAATATTTAAGTAAATGATTATTTTAATTGTTTAAAAAATACAGTATGCAGTATCAATACAAAGATGCTATCTTATTATTTTGGAGGCTCAAAGATTTGGAGAAGCTGATGGAGTTGTAGCAAGACTTGAAGTCCCAAAGCATCTGAATTTCCCTCTTGGTTTTCATGGTTTTTGGGCTGCTAATACATAAAAGGCAAATATTCATATTTGTCAAAAA

mRNA sequence

ATATCGATTTCCACTCCAAACACTGCCCGAATATCGGTCGACAACGACGATGATTCTGTTGCAGCCTTCTGGGACTACCAATTCCTCTTCGTTTCACAGCGTACCGAGACAGTAGACCCCACCGTCCTCCGCCTAGTCGACGGTGCCATACCGCCAAATTTCCCTTCCGGCACGTATTACCTGACGGGACCAGGCATGTTCTCCGACGACCATGGGTCTACGGTGCACCCACTCGACGGCCATGGGTATTTAAGAGCCTTTGTTTTCGAGAAGAAGAAGAAGGAGGTGATTTTCATGGCGAAGTACGTGAAAACAGAGGCGAAAATGGAGGAGCATGATCCGATGACTGACACGTGGCAGTTTACGCATAGAGGACCGTTTTCGGTGCTGAAGGGAGGGAAGAAATTGGGGAATACAAAGGTGATGAAAAATGTGGCTAATACGAGTGTGCTGCGGTGGGGCAGCCGCCTTCTTTGCCTTTGGGAGGGCGGCGATCCGTACGAAATTCAGCCGGAAAATTTGGATACAGTTGGGAAGTTTTCTGTTGTTCAGGGTGGCTGTGATTACGATTTGCCGTCCCCCGGCGGACCTTACGATGCTGGGTTTTGGCGAATATTCAAGATGCCACCAAAGAGGTTGTTGTCTCATTACAAGATTGATTCTCAAAGGAAAAGACTTCTCATAATGTCATGCAATGCTGAAGATATGCTTCTACCAACTAGTCATTTCACATTTTATGATTTGGTTATCAATGATCACTTGATGATCCATGATTGGGCTTTTACTGATACTCATTACATCCTCTTTGCTAACCGAATTAAGCTCGATGTCATCGGTGCAATGGGTGCTGTTAGTGGGATATCTCCAATGATATCAGCACTAAGTGTAAACACAAATAAACCCACTTCTCCAATTTATTTGATTCCAAGATTTGGTGAAGATTCAAACAAAGATTGGAGAGAAGCCATTGTTGAAGTTCCTTCAAGGCTTTGGCTACTTCATGTTGGCAATGCCTTTGAGGTTATTCATGAAGATGGCAACTTGGACTTCCAAATCCATGCCTCTGCTTGCTCCTATCAATGGTTCAATTTTAAAAAATTGTTTGGTAATGAGCTATTGCCTCACCTAGTTAAGGTTTCCATCAGTTTAAGTAAGAATGGGAAGTGTGAGAAGTGCAGTGTGGAGCCTTTGAACCAATGGACAAAAGCCTCAGATTTTCCAGTGATAAACCCTACATTCTCAGGGCTAAAACACAATTACTTATACGCTGCAACTTCCTCCGGTAATCGGCGATCGCTACCGTCGTTTCCTTTCGATATGATCGTAAAGCTCGATACTGTCACCAACACTGTCCGAACATGGTCTGCTGGTAATCGAAGATTCGTCGGAGAACCGGTTTTTGTTCCCAAAGGAGATGAAGAAGACGATGGGTATCTTCTTGTGGTTGAGTATGCAGTATCAATACAAAGATGCTATCTTATTATTTTGGAGGCTCAAAGATTTGGAGAAGCTGATGGAGTTGTAGCAAGACTTGAAGTCCCAAAGCATCTGAATTTCCCTCTTGGTTTTCATGGTTTTTGGGCTGCTAATACATAAAAGGCAAATATTCATATTTGTCAAAAA

Coding sequence (CDS)

ATATCGATTTCCACTCCAAACACTGCCCGAATATCGGTCGACAACGACGATGATTCTGTTGCAGCCTTCTGGGACTACCAATTCCTCTTCGTTTCACAGCGTACCGAGACAGTAGACCCCACCGTCCTCCGCCTAGTCGACGGTGCCATACCGCCAAATTTCCCTTCCGGCACGTATTACCTGACGGGACCAGGCATGTTCTCCGACGACCATGGGTCTACGGTGCACCCACTCGACGGCCATGGGTATTTAAGAGCCTTTGTTTTCGAGAAGAAGAAGAAGGAGGTGATTTTCATGGCGAAGTACGTGAAAACAGAGGCGAAAATGGAGGAGCATGATCCGATGACTGACACGTGGCAGTTTACGCATAGAGGACCGTTTTCGGTGCTGAAGGGAGGGAAGAAATTGGGGAATACAAAGGTGATGAAAAATGTGGCTAATACGAGTGTGCTGCGGTGGGGCAGCCGCCTTCTTTGCCTTTGGGAGGGCGGCGATCCGTACGAAATTCAGCCGGAAAATTTGGATACAGTTGGGAAGTTTTCTGTTGTTCAGGGTGGCTGTGATTACGATTTGCCGTCCCCCGGCGGACCTTACGATGCTGGGTTTTGGCGAATATTCAAGATGCCACCAAAGAGGTTGTTGTCTCATTACAAGATTGATTCTCAAAGGAAAAGACTTCTCATAATGTCATGCAATGCTGAAGATATGCTTCTACCAACTAGTCATTTCACATTTTATGATTTGGTTATCAATGATCACTTGATGATCCATGATTGGGCTTTTACTGATACTCATTACATCCTCTTTGCTAACCGAATTAAGCTCGATGTCATCGGTGCAATGGGTGCTGTTAGTGGGATATCTCCAATGATATCAGCACTAAGTGTAAACACAAATAAACCCACTTCTCCAATTTATTTGATTCCAAGATTTGGTGAAGATTCAAACAAAGATTGGAGAGAAGCCATTGTTGAAGTTCCTTCAAGGCTTTGGCTACTTCATGTTGGCAATGCCTTTGAGGTTATTCATGAAGATGGCAACTTGGACTTCCAAATCCATGCCTCTGCTTGCTCCTATCAATGGTTCAATTTTAAAAAATTGTTTGGTAATGAGCTATTGCCTCACCTAGTTAAGGTTTCCATCAGTTTAAGTAAGAATGGGAAGTGTGAGAAGTGCAGTGTGGAGCCTTTGAACCAATGGACAAAAGCCTCAGATTTTCCAGTGATAAACCCTACATTCTCAGGGCTAAAACACAATTACTTATACGCTGCAACTTCCTCCGGTAATCGGCGATCGCTACCGTCGTTTCCTTTCGATATGATCGTAAAGCTCGATACTGTCACCAACACTGTCCGAACATGGTCTGCTGGTAATCGAAGATTCGTCGGAGAACCGGTTTTTGTTCCCAAAGGAGATGAAGAAGACGATGGGTATCTTCTTGTGGTTGAGTATGCAGTATCAATACAAAGATGCTATCTTATTATTTTGGAGGCTCAAAGATTTGGAGAAGCTGATGGAGTTGTAGCAAGACTTGAAGTCCCAAAGCATCTGAATTTCCCTCTTGGTTTTCATGGTTTTTGGGCTGCTAATACATAA

Protein sequence

ISISTPNTARISVDNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVGKFSVVQGGCDYDLPSPGGPYDAGFWRIFKMPPKRLLSHYKIDSQRKRLLIMSCNAEDMLLPTSHFTFYDLVINDHLMIHDWAFTDTHYILFANRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKDWREAIVEVPSRLWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGNELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYLIILEAQRFGEADGVVARLEVPKHLNFPLGFHGFWAANT
Homology
BLAST of Lsi09G016990 vs. ExPASy Swiss-Prot
Match: Q7XJM2 (Carotenoid cleavage dioxygenase 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD7 PE=1 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.3e-203
Identity = 345/586 (58.87%), Postives = 431/586 (73.55%), Query Frame = 0

Query: 1   ISISTPNT--ARISVDNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGT 60
           ISIS P+T   R     D+ + +AF DYQ LFVSQR+ET++P V++ ++G+IP NFPSGT
Sbjct: 34  ISISIPDTGLGRTGTILDESTSSAFRDYQSLFVSQRSETIEPVVIKPIEGSIPVNFPSGT 93

Query: 61  YYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDT 120
           YYL GPG+F+DDHGSTVHPLDGHGYLRAF  +  K++  F AKYVKTEAK EEHDP+TDT
Sbjct: 94  YYLAGPGLFTDDHGSTVHPLDGHGYLRAFHIDGNKRKATFTAKYVKTEAKKEEHDPVTDT 153

Query: 121 WQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVG 180
           W+FTHRGPFSVLKGGK+ GNTKVMKNVANTSVL+W  RLLCLWEGG+PYEI+  +LDTVG
Sbjct: 154 WRFTHRGPFSVLKGGKRFGNTKVMKNVANTSVLKWAGRLLCLWEGGEPYEIESGSLDTVG 213

Query: 181 KFSVVQGG---CDYDLPSPGGPYDAGFW------------RIFKMPPKRLLSHYKIDSQR 240
           +F+V   G   CD D  S         W             +FKMPPKR LSHYK+D +R
Sbjct: 214 RFNVENNGCESCDDDDSSDRDLSGHDIWDTAADLLKPILQGVFKMPPKRFLSHYKVDGRR 273

Query: 241 KRLLIMSCNAEDMLLPTSHFTF--YD----------LVINDHLMIHDWAFTDTHYILFAN 300
           KRLL ++CNAEDMLLP S+FTF  YD            I+DH+MIHDWAFTDTHYILFAN
Sbjct: 274 KRLLTVTCNAEDMLLPRSNFTFCEYDSEFKLIQTKEFKIDDHMMIHDWAFTDTHYILFAN 333

Query: 301 RIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGED---SNKDWREAIVEVPS 360
           R+KL+ IG++ A+ G+SPM+SALS+N +  +SPIY++PRF +      +DWR   VEV S
Sbjct: 334 RVKLNPIGSIAAMCGMSPMVSALSLNPSNESSPIYILPRFSDKYSRGGRDWR-VPVEVSS 393

Query: 361 RLWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG------------------- 420
           +LWL+H GNA+E   ++G+L  QI ASACSY+WF+F+K+FG                   
Sbjct: 394 QLWLIHSGNAYETREDNGDLKIQIQASACSYRWFDFQKMFGYDWQSNKLDPSVMNLNRGD 453

Query: 421 NELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGN 480
           ++LLPHLVKVS++L   G C  C VEPLN W K SDFPVIN ++SG K+ Y+Y+A SSG 
Sbjct: 454 DKLLPHLVKVSMTLDSTGNCNSCDVEPLNGWNKPSDFPVINSSWSGKKNKYMYSAASSGT 513

Query: 481 RRSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPK-----GDEEDDGYLLVVEY 531
           R  LP FPFDM+VK D  +N VRTWS G RRFVGEP+FVPK     G+EEDDGY++VVEY
Sbjct: 514 RSELPHFPFDMVVKFDLDSNLVRTWSTGARRFVGEPMFVPKNSVEEGEEEDDGYIVVVEY 573

BLAST of Lsi09G016990 vs. ExPASy Swiss-Prot
Match: Q7XU29 (Carotenoid cleavage dioxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD7 PE=1 SV=2)

HSP 1 Score: 601.3 bits (1549), Expect = 1.1e-170
Identity = 291/562 (51.78%), Postives = 380/562 (67.62%), Query Frame = 0

Query: 17  DDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGTYYLTGPGMFSDDHGSTVH 76
           D   AAFWDY  LF SQR E +D   LR+ +GAIPP+FP+GTYYL GPG+FSDDHGSTVH
Sbjct: 47  DTLSAAFWDYNLLFRSQRDECLDSIPLRVTEGAIPPDFPAGTYYLAGPGIFSDDHGSTVH 106

Query: 77  PLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDTWQFTHRGPFSVLKGGKKL 136
           PLDGHGYLR+F F    + + + A++V+T AK EE      +W+FTHRGPFSVL+GGKK+
Sbjct: 107 PLDGHGYLRSFRFRPGDRTIHYSARFVETAAKREESRDGA-SWRFTHRGPFSVLQGGKKV 166

Query: 137 GNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVGKFSVVQGGCDYDLPSPGG 196
           GN KVMKNVANTSVLRWG RLLCLWEGG PYE+ P  L+TVG F ++      D  +   
Sbjct: 167 GNVKVMKNVANTSVLRWGGRLLCLWEGGQPYEVDPRTLETVGPFDLLGLAAADDNKATNA 226

Query: 197 PYDAGFW------------------RIFKMPPKRLLSHYKIDSQRKRLLIMSCNAEDMLL 256
                 W                   +F MP KRLL+HYKID +R RLL+++CNAEDMLL
Sbjct: 227 SAARRPWLQEAGLDAAARLLRPVLSGVFDMPGKRLLAHYKIDPRRGRLLMVACNAEDMLL 286

Query: 257 PTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRIKLDVIGAMGAVSG 316
           P SHFTFY            + V+ DHLMIHDWAFTDTHYIL  NRIKLD+ G++ A++G
Sbjct: 287 PRSHFTFYEFDAHFDLVQKREFVVPDHLMIHDWAFTDTHYILLGNRIKLDIPGSLLALTG 346

Query: 317 ISPMISALSVNTNKPTSPIYLIPRFGED--SNKDWREAIVEVPSRLWLLHVGNAFEVIHE 376
             PMI+AL+V+  + ++P+YL+PR  E     +DW    +E PS++W +HVGNAFE  + 
Sbjct: 347 THPMIAALAVDPRRQSTPVYLLPRSPETEAGGRDW-SVPIEAPSQMWSVHVGNAFEEANR 406

Query: 377 DGNLDFQIHASACSYQWFNFKKLF------------------GNELLPHLVKVSISLSKN 436
            G LD ++H S+CSYQWF+F ++F                  G E LP LV+V+I L + 
Sbjct: 407 RGGLDVRLHMSSCSYQWFHFHRMFGYNWHHKKLDPSFMNAAKGKEWLPRLVQVAIELDRT 466

Query: 437 GKCEKCSVEPL-NQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLD 496
           G+C +CSV  L +Q  + +DFP INP+++  ++ ++YA  +SG+RR LP FPFD +VK+D
Sbjct: 467 GECRRCSVRRLSDQHARPADFPAINPSYANQRNRFVYAGAASGSRRFLPYFPFDSVVKVD 526

Query: 497 TVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYLIILEAQRFGEAD 528
               + R WS   R+FVGEPVFVP G  ED GY+L+VEYAVS  RC+L++L+A++ G  +
Sbjct: 527 VSDGSARWWSTDGRKFVGEPVFVPTGGGEDGGYVLLVEYAVSKHRCHLVVLDAKKIGTEN 586

BLAST of Lsi09G016990 vs. ExPASy Swiss-Prot
Match: P74334 (Apocarotenoid-15,15'-oxygenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1541 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 1.5e-39
Identity = 142/506 (28.06%), Postives = 223/506 (44.07%), Query Frame = 0

Query: 46  VDGAIPPNFPSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKT 105
           V+G+IPP+   GT Y  GPG+         HP DG G + AF F    + V F +K+V+T
Sbjct: 38  VEGSIPPDL-QGTLYRNGPGLLEIGDRPLKHPFDGDGMVTAFKFPGDGR-VHFQSKFVRT 97

Query: 106 EAKMEEHDPMTDTWQFTHRGPFSVLKGG---KKLGNTKVMKNVANTSVLRWGSRLLCLWE 165
           +  +EE        +  +RG F     G   K + + + +KN+ANT++  WG RLL LWE
Sbjct: 98  QGYVEEQ----KAGKMIYRGVFGSQPAGGWLKTIFDLR-LKNIANTNITYWGDRLLALWE 157

Query: 166 GGDPYEIQPENLDTVGK------FSVVQGGCDYDLPSPGGPYDAGFWRIFKMPPKRLLSH 225
           GG P+ ++P NL T+G        +  Q    +    P   +D G         K  LS 
Sbjct: 158 GGQPHRLEPSNLATIGLDDLGGILAEGQPLSAHPRIDPASTFDGGQPCYVTFSIKSSLS- 217

Query: 226 YKIDSQRKRLLIMSCNAEDMLLPTSHFTFYDLVINDHLMIHDWAFTDTHYILFANRIKLD 285
                    L ++  + +  LL     TF          IHD+A T  + I   N + L+
Sbjct: 218 -------STLTLLELDPQGKLLRQKTETFPGFA-----FIHDFAITPHYAIFLQNNVTLN 277

Query: 286 VIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKDWREAIVEVPSRL-WLLHV 345
            +  +  + G       +  + +KP + I L+PR G +        I  +P +  ++ H 
Sbjct: 278 GLPYLFGLRGAG---ECVQFHPDKP-AQIILVPRDGGE--------IKRIPVQAGFVFHH 337

Query: 346 GNAFEVIHEDGNLDFQIHASAC-----------SYQWFNFKKLFGNELLPHLVKVSISLS 405
            NAFE   E+G +   I  S C            ++  NF  L   +L    +  + +  
Sbjct: 338 ANAFE---ENGKI---ILDSICYNSLPQVDTDGDFRSTNFDNLDPGQLWRFTIDPAAA-- 397

Query: 406 KNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKL 465
                   +VE     ++  +FPV++P   G  + Y+Y   +     S  + P   I+K+
Sbjct: 398 --------TVEKQLMVSRCCEFPVVHPQQVGRPYRYVYMGAA---HHSTGNAPLQAILKV 457

Query: 466 DTVTNTVRTWSAGNRRFVGEPVFVPK--GDEEDDGYLLVVEYAVSIQRCYLIILEAQRFG 525
           D  + T    S     F GEP+FVP+  G  EDDG+LL + Y   + R  L+IL+AQ   
Sbjct: 458 DLESGTETLRSFAPHGFAGEPIFVPRPGGVAEDDGWLLCLIYKADLHRSELVILDAQDI- 488

Query: 526 EADGVVARLEVPKHLNFPLGFHGFWA 529
                +A L++  H+ +PL  HG WA
Sbjct: 518 -TAPAIATLKLKHHIPYPL--HGSWA 488

BLAST of Lsi09G016990 vs. ExPASy Swiss-Prot
Match: Q9LRM7 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED6 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.2e-14
Identity = 118/511 (23.09%), Postives = 201/511 (39.33%), Query Frame = 0

Query: 52  PNFPSGTYYLTGPG-MFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKME 111
           P+   G Y   G   MF    G   H  DG G + A V      +V +  +Y KT   ++
Sbjct: 124 PSCLKGVYIRNGANPMFPPLAGH--HLFDGDGMIHA-VSIGFDNQVSYSCRYTKTNRLVQ 183

Query: 112 E-----------------HDPMTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRW 171
           E                 H  +     FT R    ++ G + +G       VAN  V+ +
Sbjct: 184 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMG-------VANAGVVFF 243

Query: 172 GSRLLCLWEGGDPYEIQPE---NLDTVGKFSVVQGGCDYDLPSPGGPYDAGFWRIFKMPP 231
             RLL + E   PY+++ +   +L+T+G+F     G D  + S                 
Sbjct: 244 NGRLLAMSEDDLPYQVKIDGQGDLETIGRF-----GFDDQIDS----------------- 303

Query: 232 KRLLSHYKIDSQRKRLLIMSCNAEDMLLPTSHFTF------------YDLVINDHLMIHD 291
             +++H K+D+    L  +S N    +L   H  +             ++ + +  MIHD
Sbjct: 304 -SVIAHPKVDATTGDLHTLSYN----VLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHD 363

Query: 292 WAFTDTHYILFANRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKD 351
           +A T+   ++   ++   V      + G SP+I                + RFG  S +D
Sbjct: 364 FAITENFVVIPDQQM---VFKLSEMIRGGSPVIYVKE-----------KMARFGVLSKQD 423

Query: 352 WREA---IVEVPSRLWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGNELLPH 411
              +    V+VP   +  H+ NA+E   E+G+    +  S  S     F +  G      
Sbjct: 424 LTGSDINWVDVPD-CFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIFSE-SGEPTRVE 483

Query: 412 LVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPS 471
           L ++ +++       K  V  +N      +   IN ++ G K  ++Y A +       P 
Sbjct: 484 LSEIRLNMRTKESNRKVIVTGVN-----LEAGHINRSYVGRKSQFVYIAIAD------PW 543

Query: 472 FPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGD-EEDDGYLLVVEYAVSIQRCYLI 526
                I K+D    TV  ++ G  RF GEP FVP+G+ EED GY++             +
Sbjct: 544 PKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPEGEGEEDKGYVMGFVRDEEKDESEFV 564

BLAST of Lsi09G016990 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 6.6e-11
Identity = 108/497 (21.73%), Postives = 199/497 (40.04%), Query Frame = 0

Query: 47  DGAIPPNFP-SGTYYLTGPGM---FSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKY 106
           +GA P + P +G ++  G GM      +HGS  +          FV E++    +F    
Sbjct: 156 NGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYAC-RFTQTNRFVQERQLGRPVF---- 215

Query: 107 VKTEAKMEEHDPMTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWE 166
            K   ++  H  +     F  R    ++      G       VAN  ++ +  RLL + E
Sbjct: 216 PKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTG-------VANAGLVYFNGRLLAMSE 275

Query: 167 GGDPYEIQ---PENLDTVGKFSVVQGGCDYDLPSPGGPYDAGFWRIFKMPPKRLLSHYKI 226
              PY++Q     +L TVG+F       D+D     G  ++            +++H K+
Sbjct: 276 DDLPYQVQITPNGDLKTVGRF-------DFD-----GQLES-----------TMIAHPKV 335

Query: 227 DSQRKRLLIMSCNAEDMLLPTSHFTF---------YDLVINDHLMIHDWAFTDTHYILFA 286
           D +   L  +S +         +F F          ++ ++   M+HD+A T+   ++  
Sbjct: 336 DPESGELFALSYDVVSKPY-LKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPD 395

Query: 287 NRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGED-SNKDWREAIVEVPSR 346
            ++   V      + G SP++     + NK  +   ++ ++ ED SN  W    ++ P  
Sbjct: 396 QQV---VFKLPEMIRGGSPVV----YDKNK-VARFGILDKYAEDSSNIKW----IDAPD- 455

Query: 347 LWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGNELLPHLVKVSISLSKNGKC 406
            +  H+ NA+    E+   D  +   +C     +        L   L ++ ++L K G+ 
Sbjct: 456 CFCFHLWNAW----EEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNL-KTGES 515

Query: 407 EKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLDTVTN 466
            +  +          +  ++N    G K  + Y A +       P        K+D  T 
Sbjct: 516 TRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAE------PWPKVSGFAKVDLTTG 575

Query: 467 TVRTWSAGNRRFVGEPVFVP-KGDEEDDGYLLVVEYAVSIQRCYLIILEAQRFGEADGVV 526
            V+    G+ R+ GEP+F+P +G EED+GY+L   +     +  L I+ A        V 
Sbjct: 576 EVKKHLYGDNRYGGEPLFLPGEGGEEDEGYILCFVHDEKTWKSELQIVNAVSL----EVE 586

BLAST of Lsi09G016990 vs. ExPASy TrEMBL
Match: A0A0A0KAN6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106700 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.0e-277
Identity = 471/578 (81.49%), Postives = 496/578 (85.81%), Query Frame = 0

Query: 1   ISISTPNTARISV---DNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSG 60
           ISISTPNT RIS+   D+DDDS+AAFWDYQFLF+SQRTET +P VLRLVDGAIP +FPSG
Sbjct: 38  ISISTPNTHRISINKNDDDDDSIAAFWDYQFLFISQRTETENPAVLRLVDGAIPSDFPSG 97

Query: 61  TYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTD 120
           TYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFE K  EV FMAKYVKTEAKMEEHDP TD
Sbjct: 98  TYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFE-KDHEVTFMAKYVKTEAKMEEHDPETD 157

Query: 121 TWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTV 180
            W+FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWG RLLCLWEGGDPYEI+ E+LDTV
Sbjct: 158 RWRFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRAEDLDTV 217

Query: 181 GKFSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQRKR 240
           GKF    GG D+D PS GG YD GFWR            +FKMPPKRLLSHYK+D+QR R
Sbjct: 218 GKFCAFHGGDDHDSPSRGGGYDGGFWRFAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNR 277

Query: 241 LLIMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRI 300
           LL+MSCNAEDMLLPTSHFTFY            D+VI+DHLMIHDWAFTD HYILFANRI
Sbjct: 278 LLVMSCNAEDMLLPTSHFTFYEFDSNFKLLQKKDMVIDDHLMIHDWAFTDNHYILFANRI 337

Query: 301 KLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNK-DWREAIVEVPSRLWL 360
           KLDVIGAM A+SGISPMISALSVNTNK TSPIYLIPRFGEDS K DW+E IVEVPSRLWL
Sbjct: 338 KLDVIGAMSAISGISPMISALSVNTNKSTSPIYLIPRFGEDSKKDDWKETIVEVPSRLWL 397

Query: 361 LHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------NELL 420
           LHVGNAFE IHEDG LDF+IHAS+CSYQWFNFKKLFG                   ++  
Sbjct: 398 LHVGNAFETIHEDGTLDFEIHASSCSYQWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQF 457

Query: 421 PHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSL 480
           PHLVKVSISLSKNGKCEKCSVEPLNQW K+SDFPVINP FSGLKHNYLYAATSSGNRRSL
Sbjct: 458 PHLVKVSISLSKNGKCEKCSVEPLNQWPKSSDFPVINPKFSGLKHNYLYAATSSGNRRSL 517

Query: 481 PSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYL 532
           PSFPFDMIVKLDTVTNTVRTW AGNRRFVGEPVFVPKGD+EDDGYLLVVEYAVSIQRCYL
Sbjct: 518 PSFPFDMIVKLDTVTNTVRTWFAGNRRFVGEPVFVPKGDKEDDGYLLVVEYAVSIQRCYL 577

BLAST of Lsi09G016990 vs. ExPASy TrEMBL
Match: T1UMH0 (Carotenoid cleavage dioxygenase OS=Cucumis melo OX=3656 GN=CCD7 PE=2 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.4e-276
Identity = 468/576 (81.25%), Postives = 496/576 (86.11%), Query Frame = 0

Query: 1   ISISTPNTARISVD-NDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGTY 60
           ISISTPNT RIS++ NDDDS+AAFWDYQFLF+SQRTET +P VLRLVDGAIPP+FPSGTY
Sbjct: 37  ISISTPNTNRISINKNDDDSIAAFWDYQFLFISQRTETENPAVLRLVDGAIPPDFPSGTY 96

Query: 61  YLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDTW 120
           YLTGPGMFSDDHGSTVHPLDGHGYLRAF FE  K EV FMAKYVKTEAKMEEHDP TD W
Sbjct: 97  YLTGPGMFSDDHGSTVHPLDGHGYLRAFAFE--KDEVAFMAKYVKTEAKMEEHDPETDRW 156

Query: 121 QFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVGK 180
           +FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWG RLLCLWEGGDPYEI+PE+LDTVGK
Sbjct: 157 RFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRPEDLDTVGK 216

Query: 181 FSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQRKRLL 240
           FS   GG D+D PS GG YD GFWR            +FKMPPKRLLSHYK+D+QR RLL
Sbjct: 217 FSAFDGGGDHDSPSRGGTYDGGFWRFAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNRLL 276

Query: 241 IMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRIKL 300
           IMSCNAEDMLLPTSHFTFY            DLVI+DHLMIHDWAFTD HYILFANRIKL
Sbjct: 277 IMSCNAEDMLLPTSHFTFYEFDSNFKLLQKKDLVIDDHLMIHDWAFTDNHYILFANRIKL 336

Query: 301 DVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKDWREAIVEVPSRLWLLHV 360
           DVIGAMGA++G+SPMISALSVNTNK TSPIYLIPRFGEDSNKD +E IVEVPSRLW+LHV
Sbjct: 337 DVIGAMGAITGVSPMISALSVNTNKSTSPIYLIPRFGEDSNKDCKETIVEVPSRLWVLHV 396

Query: 361 GNAFEVIH-EDGNLDFQIHASACSYQWFNFKKLFG-------------------NELLPH 420
           GNAFE+IH EDG LDFQIHAS+CSYQWFNFKKLFG                   ++  PH
Sbjct: 397 GNAFEIIHEEDGTLDFQIHASSCSYQWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQFPH 456

Query: 421 LVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPS 480
           LVKV ISLSKNGKCEKCSVEPL+QW K+SDFP+INP FSGLKHNYLYAATSSGNRRSLPS
Sbjct: 457 LVKVCISLSKNGKCEKCSVEPLSQWPKSSDFPIINPKFSGLKHNYLYAATSSGNRRSLPS 516

Query: 481 FPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYLII 532
           FPFDMIVKLD V +TVRTW AGNRRFVGEPVFVPKGD EDDGYLLVVEYAVSIQRCYLII
Sbjct: 517 FPFDMIVKLDAVPSTVRTWFAGNRRFVGEPVFVPKGDGEDDGYLLVVEYAVSIQRCYLII 576

BLAST of Lsi09G016990 vs. ExPASy TrEMBL
Match: A0A6J1I8R3 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472478 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.0e-260
Identity = 442/580 (76.21%), Postives = 482/580 (83.10%), Query Frame = 0

Query: 1   ISISTPNTARISVDND------DDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNF 60
           ISI +PNT++IS+++       DDS+AAFWDYQFLFVSQRTET DPTVLRLV+GAIP NF
Sbjct: 37  ISIPSPNTSQISINSPLSIPTVDDSIAAFWDYQFLFVSQRTETADPTVLRLVEGAIPENF 96

Query: 61  PSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDP 120
           PSGTYYL GPG+FSDDHGSTVHPLDGHGYLRAF+FE  KKEV FMA+YV+TEAKMEEHDP
Sbjct: 97  PSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIFE-NKKEVSFMARYVRTEAKMEEHDP 156

Query: 121 MTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENL 180
            TDTW+FTHRGPFSVLKGG+KLGNTKVMKNVANTSVL+WG RLLCLWEGGDPYEIQPE L
Sbjct: 157 ATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSVLQWGGRLLCLWEGGDPYEIQPETL 216

Query: 181 DTVGKFSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQ 240
           DTVGKFS V GGCD D PS GG + AGFWR            +FKMPP+RLLSHYK+D+Q
Sbjct: 217 DTVGKFSGVDGGCDRDSPSQGGRHGAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQ 276

Query: 241 RKRLLIMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFA 300
           R RLL+ SCNAEDMLLPTSHFTFY            + VI+DHLMIHDWAFTDTHYILFA
Sbjct: 277 RNRLLLNSCNAEDMLLPTSHFTFYEFDSNFRLLEKKEFVIDDHLMIHDWAFTDTHYILFA 336

Query: 301 NRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGE-DSNKDWREAIVEVPSR 360
           NRIKLD+ GAMGAVSG SPMIS+L VNT+K TSP+YL+PRFGE    +DWR+ I ++PS 
Sbjct: 337 NRIKLDLFGAMGAVSGASPMISSLGVNTSKSTSPVYLLPRFGEHHPTRDWRQPI-QIPSS 396

Query: 361 LWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------N 420
           LWLLHVGNAFE+    GNLDFQIHASACSYQWFNF KLFG                   +
Sbjct: 397 LWLLHVGNAFELTDPQGNLDFQIHASACSYQWFNFDKLFGYNWQTGKLDPSIMNLNGTKS 456

Query: 421 ELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNR 480
           ELLP LVKVS  LSKNG+CEKCSVEPLNQWTKASDFP INPTFSGLK+NYLYAATSSG R
Sbjct: 457 ELLPRLVKVSFRLSKNGECEKCSVEPLNQWTKASDFPTINPTFSGLKNNYLYAATSSGYR 516

Query: 481 RSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQR 531
            SLPSFPFDMIVKLD VTNT RTWSAGNRRFVGEPVFVPKGD EDDGY+LVVEYAVSIQR
Sbjct: 517 ESLPSFPFDMIVKLDMVTNTARTWSAGNRRFVGEPVFVPKGDGEDDGYVLVVEYAVSIQR 576

BLAST of Lsi09G016990 vs. ExPASy TrEMBL
Match: A0A6J1ENV6 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111436349 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.2e-259
Identity = 442/581 (76.08%), Postives = 481/581 (82.79%), Query Frame = 0

Query: 1   ISISTPNTARISVDND------DDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNF 60
           ISI + NT +IS+++       DDS+AAFWDYQFLFVSQRTET DPTVL LV+GAIP NF
Sbjct: 37  ISIPSLNTGQISINSPPSIPTVDDSIAAFWDYQFLFVSQRTETADPTVLHLVEGAIPENF 96

Query: 61  PSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDP 120
           PSGTYYL GPG+FSDDHGSTVHPLDGHGYLRAF+FE  KKEV FMAKYV+TEAKMEEHDP
Sbjct: 97  PSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIFE-NKKEVSFMAKYVRTEAKMEEHDP 156

Query: 121 MTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENL 180
            TDTW+FTHRGPFSVLKGG+KLGNTKVMKNVANTSVL+WG  LLCLWEGGDPYEIQPE L
Sbjct: 157 ATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSVLQWGGHLLCLWEGGDPYEIQPETL 216

Query: 181 DTVGKFSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQ 240
           DTVGKFS V GGCD D PS GG + AGFWR            +FKMPP+RLLSHYK+D+Q
Sbjct: 217 DTVGKFSGVDGGCDRDSPSQGGRHGAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQ 276

Query: 241 RKRLLIMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFA 300
           R RLL+ SCNAEDMLLPTSHFTFY            + VI+DHLMIHDWAFTDTHYILFA
Sbjct: 277 RNRLLLNSCNAEDMLLPTSHFTFYEFDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFA 336

Query: 301 NRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGE-DSNKDWREAIVEVPSR 360
           NRIKLD+ GAMGAVSG SPMISAL VNT+K TSP+YL+PRFGE    +DWR+ I ++PS 
Sbjct: 337 NRIKLDLFGAMGAVSGASPMISALGVNTSKSTSPVYLLPRFGEHHPTRDWRQPI-QIPSS 396

Query: 361 LWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------N 420
           LWLLHVGNAFE+ +  GNLDFQIHASACSYQWFNF KLFG                   +
Sbjct: 397 LWLLHVGNAFELTNPQGNLDFQIHASACSYQWFNFDKLFGYNWQTEKLDPSIMNLNGTKS 456

Query: 421 ELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNR 480
           ELLP LVKVSISLSKNG+CEKCSVEPLNQWTKASDFP INPTFSG+K+NYLYAATSSG R
Sbjct: 457 ELLPRLVKVSISLSKNGECEKCSVEPLNQWTKASDFPAINPTFSGMKNNYLYAATSSGYR 516

Query: 481 RSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQR 532
            SLPSFPFD IVKLDTVT T RTWSAGNRRFVGEPVFVPKGD EDDGY+LVVEYAVSIQR
Sbjct: 517 ESLPSFPFDTIVKLDTVTYTARTWSAGNRRFVGEPVFVPKGDGEDDGYVLVVEYAVSIQR 576

BLAST of Lsi09G016990 vs. ExPASy TrEMBL
Match: A0A6J1DS65 (carotenoid cleavage dioxygenase 7, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023796 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 7.1e-250
Identity = 428/570 (75.09%), Postives = 469/570 (82.28%), Query Frame = 0

Query: 14  DNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGTYYLTGPGMFSDDHGS 73
           + DDDSVAAFWDYQFLFVSQR+ET +P VLR VDGA+P +FP GTYYL GPG+F+DDHGS
Sbjct: 57  EGDDDSVAAFWDYQFLFVSQRSETAEPAVLRAVDGAVPADFPGGTYYLAGPGLFADDHGS 116

Query: 74  TVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDTWQFTHRGPFSVLKGG 133
           TVHPLDGHGYLRAF FE   KEV F AKYV+TEA+MEEH+  TDTW+FTHRGPFSVLKGG
Sbjct: 117 TVHPLDGHGYLRAFGFE--NKEVKFTAKYVRTEAQMEEHEAATDTWRFTHRGPFSVLKGG 176

Query: 134 KKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVGKFSVVQ--------G 193
           K+LGNTKVMKNVANTSVLRW  RLLCLWEGGDPYEIQP +LDT+GKFS V         G
Sbjct: 177 KRLGNTKVMKNVANTSVLRWAGRLLCLWEGGDPYEIQPGSLDTIGKFSAVDVDDDDDGGG 236

Query: 194 GCDYDLPSPGGP-YDAGFWR------------IFKMPPKRLLSHYKIDSQRKRLLIMSCN 253
           GC       G P + A  WR            +FKMPPKRLLSHYK+D+QR RLLI+SC 
Sbjct: 237 GCG----RGGSPRHGADLWRFVARLLKPLLHGVFKMPPKRLLSHYKLDAQRNRLLILSCK 296

Query: 254 AEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRIKLDVIGA 313
           AEDMLLPTS+FTFY            D VI+DHLMIHDWAFTDTHY++FANRIKLDVIGA
Sbjct: 297 AEDMLLPTSNFTFYEFDSNFKLLQKQDFVIDDHLMIHDWAFTDTHYVIFANRIKLDVIGA 356

Query: 314 MGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKDWREAIVEVPSRLWLLHVGNAFE 373
           MGAVSGISPMISALSVN +K T P+YL+PRF ED N+DWREA VEVPSRLWLLHVGNAFE
Sbjct: 357 MGAVSGISPMISALSVNGSKATCPVYLLPRFAEDGNRDWREA-VEVPSRLWLLHVGNAFE 416

Query: 374 VIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------NELLPHLVKVSI 433
           V  +DGNLD QIHASACSYQWFNFK+LFG                    +LLP LVKVSI
Sbjct: 417 VRDDDGNLDIQIHASACSYQWFNFKRLFGYNWQSGKLDPSIMNLDGTKTQLLPRLVKVSI 476

Query: 434 SLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMI 493
           SLSKNGKCEKCSVEPLNQWTKASDFP INPTFSGLK+NYLYAATSSGNRRSLPSFPFD +
Sbjct: 477 SLSKNGKCEKCSVEPLNQWTKASDFPAINPTFSGLKNNYLYAATSSGNRRSLPSFPFDTV 536

Query: 494 VKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYLIILEAQRF 532
           VKL+TVTN+VR+W AGNRRFVGEPVFVPKGDEEDDGY+LVVEYAVSIQRCYLIILEA++F
Sbjct: 537 VKLNTVTNSVRSWYAGNRRFVGEPVFVPKGDEEDDGYVLVVEYAVSIQRCYLIILEAKKF 596

BLAST of Lsi09G016990 vs. NCBI nr
Match: XP_038876141.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Benincasa hispida])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.6e-288
Identity = 486/573 (84.82%), Postives = 509/573 (88.83%), Query Frame = 0

Query: 1   ISISTPNTARISVDNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGTYY 60
           ISI TPNT RIS+ NDDDS+AAFWDYQFLFVSQRTET DPTVLRLVDGAIPP+FPSGTYY
Sbjct: 37  ISIPTPNTTRISI-NDDDSIAAFWDYQFLFVSQRTETTDPTVLRLVDGAIPPDFPSGTYY 96

Query: 61  LTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDTWQ 120
           L GPGMFSDDHGSTVHPLDGHGYLRAFVFE+KKKEV+FMAKYVKTEAKMEEHD +TDTW+
Sbjct: 97  LAGPGMFSDDHGSTVHPLDGHGYLRAFVFERKKKEVVFMAKYVKTEAKMEEHDLVTDTWR 156

Query: 121 FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVGKF 180
           FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWG RLLCLWEGGDPYEI+PENLDTVGKF
Sbjct: 157 FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRPENLDTVGKF 216

Query: 181 SVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQRKRLLI 240
           S V GGCD D PS GGPY AGFWR            +FKMPPKRLLSHYK+D+QR RLLI
Sbjct: 217 SAVHGGCDCDSPSHGGPYGAGFWRFAAELLKPVLYGVFKMPPKRLLSHYKLDAQRNRLLI 276

Query: 241 MSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRIKLD 300
           MSCNAEDMLLPTSHFTFY            DLVI+DHLMIHDWAFTDTHYILFANRIKLD
Sbjct: 277 MSCNAEDMLLPTSHFTFYEFDSNFKLLQKKDLVIDDHLMIHDWAFTDTHYILFANRIKLD 336

Query: 301 VIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKDWREAIVEVPSRLWLLHVG 360
           VIGAMGAVSGISPMISALSVNT+KPTSPIYLIPRFGEDSN++ R+A+VEVPSRLWLLHVG
Sbjct: 337 VIGAMGAVSGISPMISALSVNTSKPTSPIYLIPRFGEDSNRNLRKAVVEVPSRLWLLHVG 396

Query: 361 NAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------NELLPHLV 420
           NAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG                   +ELLP LV
Sbjct: 397 NAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGYNWQTGKLDPSIMNLNESKSELLPRLV 456

Query: 421 KVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFP 480
           K+SISL+KN KCEKCSVEPLNQWTK+SDFPVINP FSGLKHNYLYAATSSG RRSLPSFP
Sbjct: 457 KISISLNKNEKCEKCSVEPLNQWTKSSDFPVINPKFSGLKHNYLYAATSSGYRRSLPSFP 516

Query: 481 FDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYLIILE 531
           FDMIVKLD V NTVRTWSAGNRRFVGEPVFVPKG+EEDDGYLLVVEYAVSIQRCYLIILE
Sbjct: 517 FDMIVKLDMVMNTVRTWSAGNRRFVGEPVFVPKGNEEDDGYLLVVEYAVSIQRCYLIILE 576

BLAST of Lsi09G016990 vs. NCBI nr
Match: XP_004140624.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucumis sativus] >KGN46518.1 hypothetical protein Csa_005368 [Cucumis sativus])

HSP 1 Score: 963.8 bits (2490), Expect = 6.3e-277
Identity = 471/578 (81.49%), Postives = 496/578 (85.81%), Query Frame = 0

Query: 1   ISISTPNTARISV---DNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSG 60
           ISISTPNT RIS+   D+DDDS+AAFWDYQFLF+SQRTET +P VLRLVDGAIP +FPSG
Sbjct: 38  ISISTPNTHRISINKNDDDDDSIAAFWDYQFLFISQRTETENPAVLRLVDGAIPSDFPSG 97

Query: 61  TYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTD 120
           TYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFE K  EV FMAKYVKTEAKMEEHDP TD
Sbjct: 98  TYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFE-KDHEVTFMAKYVKTEAKMEEHDPETD 157

Query: 121 TWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTV 180
            W+FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWG RLLCLWEGGDPYEI+ E+LDTV
Sbjct: 158 RWRFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRAEDLDTV 217

Query: 181 GKFSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQRKR 240
           GKF    GG D+D PS GG YD GFWR            +FKMPPKRLLSHYK+D+QR R
Sbjct: 218 GKFCAFHGGDDHDSPSRGGGYDGGFWRFAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNR 277

Query: 241 LLIMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRI 300
           LL+MSCNAEDMLLPTSHFTFY            D+VI+DHLMIHDWAFTD HYILFANRI
Sbjct: 278 LLVMSCNAEDMLLPTSHFTFYEFDSNFKLLQKKDMVIDDHLMIHDWAFTDNHYILFANRI 337

Query: 301 KLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNK-DWREAIVEVPSRLWL 360
           KLDVIGAM A+SGISPMISALSVNTNK TSPIYLIPRFGEDS K DW+E IVEVPSRLWL
Sbjct: 338 KLDVIGAMSAISGISPMISALSVNTNKSTSPIYLIPRFGEDSKKDDWKETIVEVPSRLWL 397

Query: 361 LHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------NELL 420
           LHVGNAFE IHEDG LDF+IHAS+CSYQWFNFKKLFG                   ++  
Sbjct: 398 LHVGNAFETIHEDGTLDFEIHASSCSYQWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQF 457

Query: 421 PHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSL 480
           PHLVKVSISLSKNGKCEKCSVEPLNQW K+SDFPVINP FSGLKHNYLYAATSSGNRRSL
Sbjct: 458 PHLVKVSISLSKNGKCEKCSVEPLNQWPKSSDFPVINPKFSGLKHNYLYAATSSGNRRSL 517

Query: 481 PSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYL 532
           PSFPFDMIVKLDTVTNTVRTW AGNRRFVGEPVFVPKGD+EDDGYLLVVEYAVSIQRCYL
Sbjct: 518 PSFPFDMIVKLDTVTNTVRTWFAGNRRFVGEPVFVPKGDKEDDGYLLVVEYAVSIQRCYL 577

BLAST of Lsi09G016990 vs. NCBI nr
Match: NP_001284427.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucumis melo] >AGT79011.1 carotenoid cleavage dioxygenase [Cucumis melo])

HSP 1 Score: 960.3 bits (2481), Expect = 6.9e-276
Identity = 468/576 (81.25%), Postives = 496/576 (86.11%), Query Frame = 0

Query: 1   ISISTPNTARISVD-NDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGTY 60
           ISISTPNT RIS++ NDDDS+AAFWDYQFLF+SQRTET +P VLRLVDGAIPP+FPSGTY
Sbjct: 37  ISISTPNTNRISINKNDDDSIAAFWDYQFLFISQRTETENPAVLRLVDGAIPPDFPSGTY 96

Query: 61  YLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDTW 120
           YLTGPGMFSDDHGSTVHPLDGHGYLRAF FE  K EV FMAKYVKTEAKMEEHDP TD W
Sbjct: 97  YLTGPGMFSDDHGSTVHPLDGHGYLRAFAFE--KDEVAFMAKYVKTEAKMEEHDPETDRW 156

Query: 121 QFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVGK 180
           +FTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWG RLLCLWEGGDPYEI+PE+LDTVGK
Sbjct: 157 RFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGGRLLCLWEGGDPYEIRPEDLDTVGK 216

Query: 181 FSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQRKRLL 240
           FS   GG D+D PS GG YD GFWR            +FKMPPKRLLSHYK+D+QR RLL
Sbjct: 217 FSAFDGGGDHDSPSRGGTYDGGFWRFAAELLKPVLYGVFKMPPKRLLSHYKVDAQRNRLL 276

Query: 241 IMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFANRIKL 300
           IMSCNAEDMLLPTSHFTFY            DLVI+DHLMIHDWAFTD HYILFANRIKL
Sbjct: 277 IMSCNAEDMLLPTSHFTFYEFDSNFKLLQKKDLVIDDHLMIHDWAFTDNHYILFANRIKL 336

Query: 301 DVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKDWREAIVEVPSRLWLLHV 360
           DVIGAMGA++G+SPMISALSVNTNK TSPIYLIPRFGEDSNKD +E IVEVPSRLW+LHV
Sbjct: 337 DVIGAMGAITGVSPMISALSVNTNKSTSPIYLIPRFGEDSNKDCKETIVEVPSRLWVLHV 396

Query: 361 GNAFEVIH-EDGNLDFQIHASACSYQWFNFKKLFG-------------------NELLPH 420
           GNAFE+IH EDG LDFQIHAS+CSYQWFNFKKLFG                   ++  PH
Sbjct: 397 GNAFEIIHEEDGTLDFQIHASSCSYQWFNFKKLFGYNWQTGKLDPSVMNPNETKSKQFPH 456

Query: 421 LVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPS 480
           LVKV ISLSKNGKCEKCSVEPL+QW K+SDFP+INP FSGLKHNYLYAATSSGNRRSLPS
Sbjct: 457 LVKVCISLSKNGKCEKCSVEPLSQWPKSSDFPIINPKFSGLKHNYLYAATSSGNRRSLPS 516

Query: 481 FPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQRCYLII 532
           FPFDMIVKLD V +TVRTW AGNRRFVGEPVFVPKGD EDDGYLLVVEYAVSIQRCYLII
Sbjct: 517 FPFDMIVKLDAVPSTVRTWFAGNRRFVGEPVFVPKGDGEDDGYLLVVEYAVSIQRCYLII 576

BLAST of Lsi09G016990 vs. NCBI nr
Match: XP_023548386.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 910.6 bits (2352), Expect = 6.3e-261
Identity = 443/580 (76.38%), Postives = 484/580 (83.45%), Query Frame = 0

Query: 1   ISISTPNTARISVDND------DDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNF 60
           ISI +PNT +IS+++       +DS+AAFWDYQFLFVSQRTET DPTVLRLV+GAIP NF
Sbjct: 37  ISIPSPNTGQISINSPPSIPTVEDSIAAFWDYQFLFVSQRTETADPTVLRLVEGAIPENF 96

Query: 61  PSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDP 120
           PSGTYYL GPG+FSDDHGSTVHPLDGHGYLRAF+FE  KKEV FMAKYV+TEAKMEEHDP
Sbjct: 97  PSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIFE-NKKEVSFMAKYVRTEAKMEEHDP 156

Query: 121 MTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENL 180
            TDTW+FTHRGPFSVLKGG+KLGNTKVMKNVANTSVL+WG RLLCLWEGGDPYEIQPE L
Sbjct: 157 ATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSVLQWGGRLLCLWEGGDPYEIQPETL 216

Query: 181 DTVGKFSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQ 240
           DTVGKFS V GGCD D PS GG + AGFWR            +FKMPP+RLLSHYK+D+Q
Sbjct: 217 DTVGKFSGVDGGCDRDSPSQGGLHGAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQ 276

Query: 241 RKRLLIMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFA 300
           R RLL+ SCNAEDMLLPTSHFTFY            + VI+DHLMIHDWAFTDTHYILFA
Sbjct: 277 RNRLLLNSCNAEDMLLPTSHFTFYEFDSNFKLLEKKEFVIDDHLMIHDWAFTDTHYILFA 336

Query: 301 NRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGE-DSNKDWREAIVEVPSR 360
           NRIKLD+ GAMGAVSG SPMISAL VNT+K TSP+YL+PRFGE    +DWR+ I ++PS 
Sbjct: 337 NRIKLDLFGAMGAVSGASPMISALGVNTSKSTSPVYLLPRFGEHHPTRDWRQPI-QIPSS 396

Query: 361 LWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------N 420
           LWLLHVGNAFE+ +  GNLDFQIHASACSYQWFNF KLFG                   +
Sbjct: 397 LWLLHVGNAFELTNPQGNLDFQIHASACSYQWFNFDKLFGYNWQTEKLDPSIMNLNGTKS 456

Query: 421 ELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNR 480
           ELLPHLVKVSISLSKNG+CEKCSVEPLNQWTKASDFP INPTFSG+K+NYLYAATSSG R
Sbjct: 457 ELLPHLVKVSISLSKNGECEKCSVEPLNQWTKASDFPAINPTFSGMKNNYLYAATSSGYR 516

Query: 481 RSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQR 531
            SLPSFPFD IVKLDTV NT RTWSAGNRRFVGEPVFVPKGD E+DGY+LVVEYAVSIQR
Sbjct: 517 ESLPSFPFDTIVKLDTVMNTARTWSAGNRRFVGEPVFVPKGDGEEDGYVLVVEYAVSIQR 576

BLAST of Lsi09G016990 vs. NCBI nr
Match: XP_022973887.1 (carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita maxima])

HSP 1 Score: 907.9 bits (2345), Expect = 4.1e-260
Identity = 442/580 (76.21%), Postives = 482/580 (83.10%), Query Frame = 0

Query: 1   ISISTPNTARISVDND------DDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNF 60
           ISI +PNT++IS+++       DDS+AAFWDYQFLFVSQRTET DPTVLRLV+GAIP NF
Sbjct: 37  ISIPSPNTSQISINSPLSIPTVDDSIAAFWDYQFLFVSQRTETADPTVLRLVEGAIPENF 96

Query: 61  PSGTYYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDP 120
           PSGTYYL GPG+FSDDHGSTVHPLDGHGYLRAF+FE  KKEV FMA+YV+TEAKMEEHDP
Sbjct: 97  PSGTYYLAGPGLFSDDHGSTVHPLDGHGYLRAFIFE-NKKEVSFMARYVRTEAKMEEHDP 156

Query: 121 MTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENL 180
            TDTW+FTHRGPFSVLKGG+KLGNTKVMKNVANTSVL+WG RLLCLWEGGDPYEIQPE L
Sbjct: 157 ATDTWRFTHRGPFSVLKGGRKLGNTKVMKNVANTSVLQWGGRLLCLWEGGDPYEIQPETL 216

Query: 181 DTVGKFSVVQGGCDYDLPSPGGPYDAGFWR------------IFKMPPKRLLSHYKIDSQ 240
           DTVGKFS V GGCD D PS GG + AGFWR            +FKMPP+RLLSHYK+D+Q
Sbjct: 217 DTVGKFSGVDGGCDRDSPSQGGRHGAGFWRFAAELLKPVLYGVFKMPPRRLLSHYKVDAQ 276

Query: 241 RKRLLIMSCNAEDMLLPTSHFTFY------------DLVINDHLMIHDWAFTDTHYILFA 300
           R RLL+ SCNAEDMLLPTSHFTFY            + VI+DHLMIHDWAFTDTHYILFA
Sbjct: 277 RNRLLLNSCNAEDMLLPTSHFTFYEFDSNFRLLEKKEFVIDDHLMIHDWAFTDTHYILFA 336

Query: 301 NRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGE-DSNKDWREAIVEVPSR 360
           NRIKLD+ GAMGAVSG SPMIS+L VNT+K TSP+YL+PRFGE    +DWR+ I ++PS 
Sbjct: 337 NRIKLDLFGAMGAVSGASPMISSLGVNTSKSTSPVYLLPRFGEHHPTRDWRQPI-QIPSS 396

Query: 361 LWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG-------------------N 420
           LWLLHVGNAFE+    GNLDFQIHASACSYQWFNF KLFG                   +
Sbjct: 397 LWLLHVGNAFELTDPQGNLDFQIHASACSYQWFNFDKLFGYNWQTGKLDPSIMNLNGTKS 456

Query: 421 ELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNR 480
           ELLP LVKVS  LSKNG+CEKCSVEPLNQWTKASDFP INPTFSGLK+NYLYAATSSG R
Sbjct: 457 ELLPRLVKVSFRLSKNGECEKCSVEPLNQWTKASDFPTINPTFSGLKNNYLYAATSSGYR 516

Query: 481 RSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGDEEDDGYLLVVEYAVSIQR 531
            SLPSFPFDMIVKLD VTNT RTWSAGNRRFVGEPVFVPKGD EDDGY+LVVEYAVSIQR
Sbjct: 517 ESLPSFPFDMIVKLDMVTNTARTWSAGNRRFVGEPVFVPKGDGEDDGYVLVVEYAVSIQR 576

BLAST of Lsi09G016990 vs. TAIR 10
Match: AT2G44990.1 (carotenoid cleavage dioxygenase 7 )

HSP 1 Score: 677.2 bits (1746), Expect = 1.1e-194
Identity = 335/586 (57.17%), Postives = 423/586 (72.18%), Query Frame = 0

Query: 1   ISISTPNT--ARISVDNDDDSVAAFWDYQFLFVSQRTETVDPTVLRLVDGAIPPNFPSGT 60
           ISIS P+T   R     D+ + +AF DYQ LFVSQR+ET++P V++ ++G+IP NFPSGT
Sbjct: 34  ISISIPDTGLGRTGTILDESTSSAFRDYQSLFVSQRSETIEPVVIKPIEGSIPVNFPSGT 93

Query: 61  YYLTGPGMFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEEHDPMTDT 120
           YYL GPG+F+DDHGSTVHPLDGHGYLRAF  +  K++  F AKYVKTEAK EEHDP+TDT
Sbjct: 94  YYLAGPGLFTDDHGSTVHPLDGHGYLRAFHIDGNKRKATFTAKYVKTEAKKEEHDPVTDT 153

Query: 121 WQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEIQPENLDTVG 180
           W+FTHRGPFSVLKGGK+ GNTKVMKNVANTSVL+W  RLLCLWEGG+PYEI+  +LDTVG
Sbjct: 154 WRFTHRGPFSVLKGGKRFGNTKVMKNVANTSVLKWAGRLLCLWEGGEPYEIESGSLDTVG 213

Query: 181 KFSVVQGG---CDYDLPSPGGPYDAGFW------------RIFKMPPKRLLSHYKIDSQR 240
           +F+V   G   CD D  S         W             +FKMPPKR LSHYK+D +R
Sbjct: 214 RFNVENNGCESCDDDDSSDRDLSGHDIWDTAADLLKPILQGVFKMPPKRFLSHYKVDGRR 273

Query: 241 KRLLIMSCNAEDMLLPTSHFTF--YD----------LVINDHLMIHDWAFTDTHYILFAN 300
           KRLL ++CNAEDMLLP S+FTF  YD            I+DH+MIHDWAFTDTHYILFAN
Sbjct: 274 KRLLTVTCNAEDMLLPRSNFTFCEYDSEFKLIQTKEFKIDDHMMIHDWAFTDTHYILFAN 333

Query: 301 RIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGED---SNKDWREAIVEVPS 360
           R+KL+ IG++ A+ G+SPM+SALS+N +  +SPIY++PRF +      +DWR   VEV S
Sbjct: 334 RVKLNPIGSIAAMCGMSPMVSALSLNPSNESSPIYILPRFSDKYSRGGRDWR-VPVEVSS 393

Query: 361 RLWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFG------------------- 420
           +LWL+H GNA+E   ++G+L  QI ASACSY+WF+F+K+FG                   
Sbjct: 394 QLWLIHSGNAYETREDNGDLKIQIQASACSYRWFDFQKMFGYDWQSNKLDPSVMNLNRGD 453

Query: 421 NELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGN 480
           ++LLPHLVKVS++L   G C  C VEPLN W K SDFPVIN ++SG K+ Y+Y+A SSG 
Sbjct: 454 DKLLPHLVKVSMTLDSTGNCNSCDVEPLNGWNKPSDFPVINSSWSGKKNKYMYSAASSGT 513

Query: 481 RRSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPK-----GDEEDDGYLLVVEY 528
           R  LP FPFDM+VK D  +N VRTWS G RRFVGEP+FVPK     G+EEDDGY++VVEY
Sbjct: 514 RSELPHFPFDMVVKFDLDSNLVRTWSTGARRFVGEPMFVPKNSVEEGEEEDDGYIVVVEY 573

BLAST of Lsi09G016990 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-15
Identity = 118/511 (23.09%), Postives = 201/511 (39.33%), Query Frame = 0

Query: 52  PNFPSGTYYLTGPG-MFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKME 111
           P+   G Y   G   MF    G   H  DG G + A V      +V +  +Y KT   ++
Sbjct: 124 PSCLKGVYIRNGANPMFPPLAGH--HLFDGDGMIHA-VSIGFDNQVSYSCRYTKTNRLVQ 183

Query: 112 E-----------------HDPMTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRW 171
           E                 H  +     FT R    ++ G + +G       VAN  V+ +
Sbjct: 184 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMG-------VANAGVVFF 243

Query: 172 GSRLLCLWEGGDPYEIQPE---NLDTVGKFSVVQGGCDYDLPSPGGPYDAGFWRIFKMPP 231
             RLL + E   PY+++ +   +L+T+G+F     G D  + S                 
Sbjct: 244 NGRLLAMSEDDLPYQVKIDGQGDLETIGRF-----GFDDQIDS----------------- 303

Query: 232 KRLLSHYKIDSQRKRLLIMSCNAEDMLLPTSHFTF------------YDLVINDHLMIHD 291
             +++H K+D+    L  +S N    +L   H  +             ++ + +  MIHD
Sbjct: 304 -SVIAHPKVDATTGDLHTLSYN----VLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHD 363

Query: 292 WAFTDTHYILFANRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDSNKD 351
           +A T+   ++   ++   V      + G SP+I                + RFG  S +D
Sbjct: 364 FAITENFVVIPDQQM---VFKLSEMIRGGSPVIYVKE-----------KMARFGVLSKQD 423

Query: 352 WREA---IVEVPSRLWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGNELLPH 411
              +    V+VP   +  H+ NA+E   E+G+    +  S  S     F +  G      
Sbjct: 424 LTGSDINWVDVPD-CFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIFSE-SGEPTRVE 483

Query: 412 LVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPS 471
           L ++ +++       K  V  +N      +   IN ++ G K  ++Y A +       P 
Sbjct: 484 LSEIRLNMRTKESNRKVIVTGVN-----LEAGHINRSYVGRKSQFVYIAIAD------PW 543

Query: 472 FPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKGD-EEDDGYLLVVEYAVSIQRCYLI 526
                I K+D    TV  ++ G  RF GEP FVP+G+ EED GY++             +
Sbjct: 544 PKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPEGEGEEDKGYVMGFVRDEEKDESEFV 564

BLAST of Lsi09G016990 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 70.5 bits (171), Expect = 4.7e-12
Identity = 108/497 (21.73%), Postives = 199/497 (40.04%), Query Frame = 0

Query: 47  DGAIPPNFP-SGTYYLTGPGM---FSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKY 106
           +GA P + P +G ++  G GM      +HGS  +          FV E++    +F    
Sbjct: 156 NGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYAC-RFTQTNRFVQERQLGRPVF---- 215

Query: 107 VKTEAKMEEHDPMTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWE 166
            K   ++  H  +     F  R    ++      G       VAN  ++ +  RLL + E
Sbjct: 216 PKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTG-------VANAGLVYFNGRLLAMSE 275

Query: 167 GGDPYEIQ---PENLDTVGKFSVVQGGCDYDLPSPGGPYDAGFWRIFKMPPKRLLSHYKI 226
              PY++Q     +L TVG+F       D+D     G  ++            +++H K+
Sbjct: 276 DDLPYQVQITPNGDLKTVGRF-------DFD-----GQLES-----------TMIAHPKV 335

Query: 227 DSQRKRLLIMSCNAEDMLLPTSHFTF---------YDLVINDHLMIHDWAFTDTHYILFA 286
           D +   L  +S +         +F F          ++ ++   M+HD+A T+   ++  
Sbjct: 336 DPESGELFALSYDVVSKPY-LKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPD 395

Query: 287 NRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGED-SNKDWREAIVEVPSR 346
            ++   V      + G SP++     + NK  +   ++ ++ ED SN  W    ++ P  
Sbjct: 396 QQV---VFKLPEMIRGGSPVV----YDKNK-VARFGILDKYAEDSSNIKW----IDAPD- 455

Query: 347 LWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGNELLPHLVKVSISLSKNGKC 406
            +  H+ NA+    E+   D  +   +C     +        L   L ++ ++L K G+ 
Sbjct: 456 CFCFHLWNAW----EEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNL-KTGES 515

Query: 407 EKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLDTVTN 466
            +  +          +  ++N    G K  + Y A +       P        K+D  T 
Sbjct: 516 TRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAE------PWPKVSGFAKVDLTTG 575

Query: 467 TVRTWSAGNRRFVGEPVFVP-KGDEEDDGYLLVVEYAVSIQRCYLIILEAQRFGEADGVV 526
            V+    G+ R+ GEP+F+P +G EED+GY+L   +     +  L I+ A        V 
Sbjct: 576 EVKKHLYGDNRYGGEPLFLPGEGGEEDEGYILCFVHDEKTWKSELQIVNAVSL----EVE 586

BLAST of Lsi09G016990 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 69.7 bits (169), Expect = 8.0e-12
Identity = 114/513 (22.22%), Postives = 198/513 (38.60%), Query Frame = 0

Query: 46  VDGAIPPNFPSGTYYLTGPG-MFSDDHGSTVHPLDGHGYLRAFVFEKKKKEVIFMAKYVK 105
           VDG I P+   G Y   G   +F    G   H  DG G + A   +    +  +  ++ +
Sbjct: 131 VDGKI-PDCIDGVYLRNGANPLFEPVSGH--HLFDGDGMVHA--VKITNGDASYSCRFTE 190

Query: 106 TEAKMEE-----------------HDPMTDTWQFTHRGPFSVLKGGKKLGNTKVMKNVAN 165
           TE  ++E                 H  +     F  RG F +L       N K    VAN
Sbjct: 191 TERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLL-------NHKNGTGVAN 250

Query: 166 TSVLRWGSRLLCLWEGGDPYEIQPE---NLDTVGKFSVVQGGCDYDLPSPGGPYDAGFWR 225
             ++ +  RLL + E   PY+++     +L+T+G+F       D+D     G   +    
Sbjct: 251 AGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRF-------DFD-----GQLSSA--- 310

Query: 226 IFKMPPKRLLSHYKIDSQRKRLLIMSCNAEDMLLPTSHFTF---------YDLVINDHLM 285
                   +++H KID   K L  +S +         +F F          ++ +    M
Sbjct: 311 --------MIAHPKIDPVTKELFALSYDVVKKPY-LKYFKFSPEGEKSPDVEIPLASPTM 370

Query: 286 IHDWAFTDTHYILFANRIKLDVIGAMGAVSGISPMISALSVNTNKPTSPIYLIPRFGEDS 345
           +HD+A T+   ++   ++   V        G SP+         +  S   ++PR  +D+
Sbjct: 371 MHDFAITENFVVIPDQQV---VFKLSDMFLGKSPV-----KYDGEKISRFGILPRNAKDA 430

Query: 346 NKDWREAIVEVPSRLWLLHVGNAFEVIHEDGNLDFQIHASACSYQWFNFKKLFGNELLPH 405
           ++      VE P   +  H+ NA+    E    D  +   +C     +       +L   
Sbjct: 431 SE---MVWVESP-ETFCFHLWNAW----ESPETDEVVVIGSCMTPADSIFNECDEQLNSV 490

Query: 406 LVKVSISLSKNGKCEKCSVEPLNQWTKASDFPVINPTFSGLKHNYLYAATSSGNRRSLPS 465
           L ++ ++L K GK  + ++ P        +  ++N    G K  Y Y A +       P 
Sbjct: 491 LSEIRLNL-KTGKSTRRTIIP-GSVQMNLEAGMVNRNLLGRKTRYAYLAIAE------PW 550

Query: 466 FPFDMIVKLDTVTNTVRTWSAGNRRFVGEPVFVPKG---DEEDDGYLLVVEYAVSIQRCY 525
                  K+D  T  V+    G +++ GEP F+P+G   D EDDGY++   +        
Sbjct: 551 PKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESE 577

BLAST of Lsi09G016990 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 68.6 bits (166), Expect = 1.8e-11
Identity = 104/480 (21.67%), Postives = 182/480 (37.92%), Query Frame = 0

Query: 76  HPLDGHGYLRAFVFEKKKKEVIFMAKYVKTEAKMEE-----------------HDPMTDT 135
           H  DG G + A  F+     V +  ++ +T   ++E                 H  +   
Sbjct: 229 HLFDGDGMVHAVRFD--NGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKL 288

Query: 136 WQFTHRGPFSVLKGGKKLGNTKVMKNVANTSVLRWGSRLLCLWEGGDPYEI---QPENLD 195
             F  RG F ++     LG       VAN  ++ +   LL + E   PY +   Q  +L+
Sbjct: 289 MLFNTRGLFGLVDPTGGLG-------VANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLE 348

Query: 196 TVGKFSVVQGGCDYDLPSPGGPYDAGFWRIFKMPPKRLLSHYKIDSQRKRLLIMSCN-AE 255
           T G++       D+D                      +++H KID + + L  +S +   
Sbjct: 349 TSGRY-------DFD----------------GQLKSTMIAHPKIDPETRELFALSYDVVS 408

Query: 256 DMLLPTSHFTF-------YDLVINDHLMIHDWAFTDTHYILFANRIKLDVIGAMGAVSGI 315
              L    FT         ++ ++   MIHD+A T+   ++   ++   V      + G 
Sbjct: 409 KPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQV---VFRLPEMIRGG 468

Query: 316 SPMISALSVNTNKPTSPIYLIPRFGED-SNKDWREAIVEVPSRLWLLHVGNAFEVIHEDG 375
           SP+     V   K  S   ++ +  +D S+  W    +EVP   +  H+ N++    E+ 
Sbjct: 469 SPV-----VYDEKKKSRFGILNKNAKDASSIQW----IEVPD-CFCFHLWNSW----EEP 528

Query: 376 NLDFQIHASACSYQWFNFKKLFGNELLPHLVKVSISLSKNGKCEKCSVEPLNQWTKASDF 435
             D  +   +C            NE    L  V   +  N K  + +  P+       + 
Sbjct: 529 ETDEVVVIGSC----MTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVISEQVNLEA 588

Query: 436 PVINPTFSGLKHNYLYAATSSGNRRSLPSFPFDMIVKLDTVTNTVRTWSAGNRRFVGEPV 495
            ++N    G K  Y Y A +       P        K+D  T  +R +  G  ++ GEP+
Sbjct: 589 GMVNRNLLGRKTRYAYLALTE------PWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPL 643

Query: 496 FVPKGD-EEDDGYLLVVEYAVSIQRCYLIILEAQRFGEADGVVARLEVPKHLNFPLGFHG 526
           F+P GD EED GY++V  +     +  L ++ A        + A + +P  +  P GFHG
Sbjct: 649 FLPSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNM----KLEATVTLPSRV--PYGFHG 643

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XJM21.3e-20358.87Carotenoid cleavage dioxygenase 7, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q7XU291.1e-17051.78Carotenoid cleavage dioxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica... [more]
P743341.5e-3928.06Apocarotenoid-15,15'-oxygenase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) O... [more]
Q9LRM72.2e-1423.099-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9LRR76.6e-1121.739-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A0A0KAN63.0e-27781.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106700 PE=3 SV=1[more]
T1UMH03.4e-27681.25Carotenoid cleavage dioxygenase OS=Cucumis melo OX=3656 GN=CCD7 PE=2 SV=1[more]
A0A6J1I8R32.0e-26076.21carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1ENV62.2e-25976.08carotenoid cleavage dioxygenase 7, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1DS657.1e-25075.09carotenoid cleavage dioxygenase 7, chloroplastic OS=Momordica charantia OX=3673 ... [more]
Match NameE-valueIdentityDescription
XP_038876141.14.6e-28884.82carotenoid cleavage dioxygenase 7, chloroplastic [Benincasa hispida][more]
XP_004140624.16.3e-27781.49carotenoid cleavage dioxygenase 7, chloroplastic [Cucumis sativus] >KGN46518.1 h... [more]
NP_001284427.16.9e-27681.25carotenoid cleavage dioxygenase 7, chloroplastic [Cucumis melo] >AGT79011.1 caro... [more]
XP_023548386.16.3e-26176.38carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022973887.14.1e-26076.21carotenoid cleavage dioxygenase 7, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G44990.11.1e-19457.17carotenoid cleavage dioxygenase 7 [more]
AT3G24220.11.6e-1523.09nine-cis-epoxycarotenoid dioxygenase 6 [more]
AT3G14440.14.7e-1221.73nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G30100.18.0e-1222.22nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT1G78390.11.8e-1121.67nine-cis-epoxycarotenoid dioxygenase 9 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 31..528
e-value: 1.7E-92
score: 311.0
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 13..529
NoneNo IPR availablePANTHERPTHR10543:SF37CAROTENOID CLEAVAGE DIOXYGENASE 7, CHLOROPLASTICcoord: 13..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G016990.1Lsi09G016990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
biological_process GO:0009733 response to auxin
biological_process GO:0010223 secondary shoot formation
biological_process GO:1901601 strigolactone biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045549 9-cis-epoxycarotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen