Lsi09G016730 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G016730
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionkinesin light chain 1-like
Locationchr09: 25169264 .. 25173003 (+)
RNA-Seq ExpressionLsi09G016730
SyntenyLsi09G016730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTCCTCTCCCAAATTCAGAATTCTCACAAACTTCACCAATTTGCATCATCAAAATGGCAATAGAAACACAAACACCCCATCTTCACATTCAAATCCCACCCATAGAAAACGATGACACACAACATCAGAACAACAATTACAAAACCATGACACCACCATGGCTAAAACCCATTCCCTCTCCATCTCAATCTCCACAAAAGTTGGGACCGAACCAAACCTGGCTCGACAACCCAGACCACGGTCCATACCTACTAAAGCTAGCTCGGGACACAATAATTTCGGGCGAGAGTCCGACCAAGGCCTTGGACTACGCGGTCCGAGCTGCCAAGTCGTTCGAGAGATTTCCGAGCCTCGGCGTCGAGCTGCCCATGAGCTTGCACGTGGTCGCTGCCATTCACTGCCGGCTCGGGCAGTTTGATGAGGCCATTCCGGTTCTTGAACAGTCCATCGAGGTTGCGGAACCCGGTAACGGACCAGACCATGCTTTGGCGAAATATTCAGGGTACATGCAGCTTGGGGATACTTATTCCATGCTGGGGCAGTTGGATCGGTCCATTTCTTGTTATGAGGATGGTTTGATGATCCAGATTAATGCTTTTACTGATTCTGACCCTCAAGTTGCTGAGACTTGCAGGTATTAACTTGTTTACTTGCTTACTCTATATATATATATATATATATATATATATATATATATATATATATATATTTTGATCTCTTATTAGATGATTTGAGTAATTTTATTTTCCAATTTCTAATTTGATCAATCATATATGCTTTAGATTACAAGGATGACTCAGTGAAAGCGTGAAAAAAAAATGTTAAAATACTATTTCGGTTCATATAGTTTGAGTTTCATTTTCACATTTGATTTCATATACTTTTAATCAATCTTAAAATCAATCATTCGAAGTTAGAATTTATTTAAAAAATTTAAAACATTTTAAAATTTTATACAAATAAGACTAATGGAGTTTTTTTTTTAAAAAAAAATTAACAAAATAGGACCTAAACTATTGGTAAGTTTTATTAAATATACATAGACTAAAATAGAATAAGTTCCAAAATAAACTAACTAAACCGATATTACATATAATACTTCATTTGTTCATGTAATATATTATTTATCTAAAATTTAAAAGGAAAAAACTTAAGTGCAATCAGGCTGTATACATCTCTTGTGCCGCCCCTGTTGCACTCAATTAAAAATTGACACGTGTCAATTTTTTTAATGATCTTTTTTTGTGCGTATGTGTGATTAAAAGAAGGAGAACATGTGCAGTCTAATATAGTTGAAAGTTAAAATGCAGAGAATAAAGAAGGTCATTATCATTATTCCATTACTAAGAGTTAGTACAAAATAACAGGTACTTAGCGGAGGCAAATGTACAAGCAATGCAATTCGAAAAGGCAAAGAAATACTGCAAGAAAACACTAGAAATTCACAAACAACACAGTCCACCAGCATCCCCACAAGAAGCAACTGACCGTCGTCTAATGGCTTTAATTTGTGAGGCTTTAGGTGATTCCGAGTCAGCTTTAGAGCACCTCGTCCTAGCCAGCATGGCAATGCTTGCCCACGGCCACGACACCGAGGTCGCTGCCATCGACGCTAGCATTGGCGACATCTACACCTCCCTCTGCCGCTTCGACGAGGCCATATTTGCCTACCAAAAAGCCCTCACAATCTTCAACTCCACAAAAGGGGACTCTCACCTCTCTGTCGCCTCGCTCTTCATACGCCTCGCTGAGCTCTACAACCGCACCAGCAAGTCCCGAGAGGCGAAGTCGTATGCAGACAATGCATTGAGAATCTACCTAAAGCCTCCAATGGGGGCTTCATGCGAAGAGATTTCGAGTGGGCTAGTGGAAATCTCGGCCGTGTATGAAGCCGCGAACGAACCGGAGGAGGCATTTGGGGTTCTACAAAGGGCGAGAAAGATATTGGAGCGTGGCGGAGGAGGCGGACGAGGGAGCGGGAGCAGGGCGGTGGCCGGGATAGAGGCTCAAATGGGGGTGATGTTTTATATGGTGGGGAAGTATGGAGAGGCTAGGAAGGCATTTGAAGGGGCAATTGGGAAGCTAAGAGGAGGTGGGGCTAAATCAGCAATGTTTGGGGTTTTGTTGAATCAAATGGGATTGGCTTGTATTCAGCTCTACAAAATTGGGGAGGCAATTAAGCTGTTTCAAGAGGCCAAATTGGTGTTAGAGGAACAATATGGTGTCTACCATTCTGATACTCTTGCTGTTTCTAGCAATCTTGCTGCTGCTTATGATGCTATGGGAAGGTATAACATTTATATATACTATATATTTTTTTTTAGTTCAATACGCTGTCACTAATGTCATCTTTAGGAATGTTCTGAAATGGTTAAAATCACTTTTGTCATTTTAAAACTATTTAGAAATGTAACATACCTTTAATCACTCAAAAATCAATTTTAAAATTAAAGTGTAAAAATTAAATATTAAATTGATTTTGAGTGATTAAATGTACGTTTCAGAGTGATTTTAACAATTTAAAAATCACTTCTAAATATGAGAAAAGTTTAGGTACAAATATGAATCAGAATACAATACAGGGAGATAAAAAGGAAAAATTATTGCTTAAACTATATAACGTAAAAAAAGAAAGCGTAGGGAGTCTAAACGTTTGACCATTTTTGTATTATTTTTAACAACTCAAGGGTAAAAATGTAATTTCACTCCTAAAAAAAGTAGGAAAGAAACCACTTTTTAAGAATCAGGCCCTTTTTCGTAGAAGTTATTTGTTTTTAGGTTTTAGCTCGAGAAAATTTTCAGATTAAAATGAGTTCTTTCGTTATTACAAGTGTGATCCGTGAACGTTTAGTTTTGTGTTTATTTGATTCTTAAACTTTAAAAAAGTGTGTAATATACCGTCTCTAGTCTTTTACATTATTTGTTCAATAGATCTTTGACATATTCGAATTTCTTAAAAATTAAATTATTCATTAAATATAAATTTGAATTTTAAATAGAATGGAATAACTACAAACTTTTGATTTTGTATCTAGTAAAATCGTGAATAGATCTAAGATCTTATTAAATTAAAAAAAAATTAAACTCTAGAAACTATTTTACACAAACTAAACGTTTAAAAACTTGTTAGACACGTCTTAAACTTCACAAATCTATTAAACACAAAATTAAAAATTTAGAAGATACTTTAAAGTTCAAGGCTCAAACAGAAACGTTATCAAACGGACTCTAAATTTGTAGTTTTTAAAAATATTATAGTCAATAATTATTATATATGCAGGGTGGAAGAAGCAATTGAGATATTAGAAGACATACTTAAAGTGAGAGAAGAAATGCTTGGAACTGCAAATCCAGAGGTAGATGAGGAAAAAAGAAGGCTAGAGGAATTGTTAAAGGAAGCAGGAAGGGCAAGGAATAAAAAGGCAAAGTCATTACAAATTTTGCTTGGCTCTAACTCCCTAAGGATTAAGAAAGAAGTAGCAAAGAGATGGTCTGGTGGCTTTAGTTTTAGAGCTTGACAAAATTTCCCTTTATTATTCTTTCTTCACTCTAATTTCCTTTTCCCACATTAATTTACTATGAAATTCTTTAGAAAAAGAACAATTCATTCCATAATTATCATAGAACAATTAAATAATATATAATGTGAGGGGGCGATACTTTGAGTTTATAGTTTGTGTCTTTCCACATCATGTGGTTGCAACATTGTTTGGAAATTCCACATCATAAGGTTTCTTC

mRNA sequence

AATTCCTCTCCCAAATTCAGAATTCTCACAAACTTCACCAATTTGCATCATCAAAATGGCAATAGAAACACAAACACCCCATCTTCACATTCAAATCCCACCCATAGAAAACGATGACACACAACATCAGAACAACAATTACAAAACCATGACACCACCATGGCTAAAACCCATTCCCTCTCCATCTCAATCTCCACAAAAGTTGGGACCGAACCAAACCTGGCTCGACAACCCAGACCACGGTCCATACCTACTAAAGCTAGCTCGGGACACAATAATTTCGGGCGAGAGTCCGACCAAGGCCTTGGACTACGCGGTCCGAGCTGCCAAGTCGTTCGAGAGATTTCCGAGCCTCGGCGTCGAGCTGCCCATGAGCTTGCACGTGGTCGCTGCCATTCACTGCCGGCTCGGGCAGTTTGATGAGGCCATTCCGGTTCTTGAACAGTCCATCGAGGTTGCGGAACCCGGTAACGGACCAGACCATGCTTTGGCGAAATATTCAGGGTACATGCAGCTTGGGGATACTTATTCCATGCTGGGGCAGTTGGATCGGTCCATTTCTTGTTATGAGGATGGTTTGATGATCCAGATTAATGCTTTTACTGATTCTGACCCTCAAGTTGCTGAGACTTGCAGGTACTTAGCGGAGGCAAATGTACAAGCAATGCAATTCGAAAAGGCAAAGAAATACTGCAAGAAAACACTAGAAATTCACAAACAACACAGTCCACCAGCATCCCCACAAGAAGCAACTGACCGTCGTCTAATGGCTTTAATTTGTGAGGCTTTAGGTGATTCCGAGTCAGCTTTAGAGCACCTCGTCCTAGCCAGCATGGCAATGCTTGCCCACGGCCACGACACCGAGGTCGCTGCCATCGACGCTAGCATTGGCGACATCTACACCTCCCTCTGCCGCTTCGACGAGGCCATATTTGCCTACCAAAAAGCCCTCACAATCTTCAACTCCACAAAAGGGGACTCTCACCTCTCTGTCGCCTCGCTCTTCATACGCCTCGCTGAGCTCTACAACCGCACCAGCAAGTCCCGAGAGGCGAAGTCGTATGCAGACAATGCATTGAGAATCTACCTAAAGCCTCCAATGGGGGCTTCATGCGAAGAGATTTCGAGTGGGCTAGTGGAAATCTCGGCCGTGTATGAAGCCGCGAACGAACCGGAGGAGGCATTTGGGGTTCTACAAAGGGCGAGAAAGATATTGGAGCGTGGCGGAGGAGGCGGACGAGGGAGCGGGAGCAGGGCGGTGGCCGGGATAGAGGCTCAAATGGGGGTGATGTTTTATATGGTGGGGAAGTATGGAGAGGCTAGGAAGGCATTTGAAGGGGCAATTGGGAAGCTAAGAGGAGGTGGGGCTAAATCAGCAATGTTTGGGGTTTTGTTGAATCAAATGGGATTGGCTTGTATTCAGCTCTACAAAATTGGGGAGGCAATTAAGCTGTTTCAAGAGGCCAAATTGGTGTTAGAGGAACAATATGGTGTCTACCATTCTGATACTCTTGCTGTTTCTAGCAATCTTGCTGCTGCTTATGATGCTATGGGAAGGGTGGAAGAAGCAATTGAGATATTAGAAGACATACTTAAAGTGAGAGAAGAAATGCTTGGAACTGCAAATCCAGAGGTAGATGAGGAAAAAAGAAGGCTAGAGGAATTGTTAAAGGAAGCAGGAAGGGCAAGGAATAAAAAGGCAAAGTCATTACAAATTTTGCTTGGCTCTAACTCCCTAAGGATTAAGAAAGAAGTAGCAAAGAGATGGTCTGGTGGCTTTAGTTTTAGAGCTTGACAAAATTTCCCTTTATTATTCTTTCTTCACTCTAATTTCCTTTTCCCACATTAATTTACTATGAAATTCTTTAGAAAAAGAACAATTCATTCCATAATTATCATAGAACAATTAAATAATATATAATGTGAGGGGGCGATACTTTGAGTTTATAGTTTGTGTCTTTCCACATCATGTGGTTGCAACATTGTTTGGAAATTCCACATCATAAGGTTTCTTC

Coding sequence (CDS)

ATGGCAATAGAAACACAAACACCCCATCTTCACATTCAAATCCCACCCATAGAAAACGATGACACACAACATCAGAACAACAATTACAAAACCATGACACCACCATGGCTAAAACCCATTCCCTCTCCATCTCAATCTCCACAAAAGTTGGGACCGAACCAAACCTGGCTCGACAACCCAGACCACGGTCCATACCTACTAAAGCTAGCTCGGGACACAATAATTTCGGGCGAGAGTCCGACCAAGGCCTTGGACTACGCGGTCCGAGCTGCCAAGTCGTTCGAGAGATTTCCGAGCCTCGGCGTCGAGCTGCCCATGAGCTTGCACGTGGTCGCTGCCATTCACTGCCGGCTCGGGCAGTTTGATGAGGCCATTCCGGTTCTTGAACAGTCCATCGAGGTTGCGGAACCCGGTAACGGACCAGACCATGCTTTGGCGAAATATTCAGGGTACATGCAGCTTGGGGATACTTATTCCATGCTGGGGCAGTTGGATCGGTCCATTTCTTGTTATGAGGATGGTTTGATGATCCAGATTAATGCTTTTACTGATTCTGACCCTCAAGTTGCTGAGACTTGCAGGTACTTAGCGGAGGCAAATGTACAAGCAATGCAATTCGAAAAGGCAAAGAAATACTGCAAGAAAACACTAGAAATTCACAAACAACACAGTCCACCAGCATCCCCACAAGAAGCAACTGACCGTCGTCTAATGGCTTTAATTTGTGAGGCTTTAGGTGATTCCGAGTCAGCTTTAGAGCACCTCGTCCTAGCCAGCATGGCAATGCTTGCCCACGGCCACGACACCGAGGTCGCTGCCATCGACGCTAGCATTGGCGACATCTACACCTCCCTCTGCCGCTTCGACGAGGCCATATTTGCCTACCAAAAAGCCCTCACAATCTTCAACTCCACAAAAGGGGACTCTCACCTCTCTGTCGCCTCGCTCTTCATACGCCTCGCTGAGCTCTACAACCGCACCAGCAAGTCCCGAGAGGCGAAGTCGTATGCAGACAATGCATTGAGAATCTACCTAAAGCCTCCAATGGGGGCTTCATGCGAAGAGATTTCGAGTGGGCTAGTGGAAATCTCGGCCGTGTATGAAGCCGCGAACGAACCGGAGGAGGCATTTGGGGTTCTACAAAGGGCGAGAAAGATATTGGAGCGTGGCGGAGGAGGCGGACGAGGGAGCGGGAGCAGGGCGGTGGCCGGGATAGAGGCTCAAATGGGGGTGATGTTTTATATGGTGGGGAAGTATGGAGAGGCTAGGAAGGCATTTGAAGGGGCAATTGGGAAGCTAAGAGGAGGTGGGGCTAAATCAGCAATGTTTGGGGTTTTGTTGAATCAAATGGGATTGGCTTGTATTCAGCTCTACAAAATTGGGGAGGCAATTAAGCTGTTTCAAGAGGCCAAATTGGTGTTAGAGGAACAATATGGTGTCTACCATTCTGATACTCTTGCTGTTTCTAGCAATCTTGCTGCTGCTTATGATGCTATGGGAAGGGTGGAAGAAGCAATTGAGATATTAGAAGACATACTTAAAGTGAGAGAAGAAATGCTTGGAACTGCAAATCCAGAGGTAGATGAGGAAAAAAGAAGGCTAGAGGAATTGTTAAAGGAAGCAGGAAGGGCAAGGAATAAAAAGGCAAAGTCATTACAAATTTTGCTTGGCTCTAACTCCCTAAGGATTAAGAAAGAAGTAGCAAAGAGATGGTCTGGTGGCTTTAGTTTTAGAGCTTGA

Protein sequence

MAIETQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTWLDNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLGVELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKEVAKRWSGGFSFRA
Homology
BLAST of Lsi09G016730 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 2.4e-184
Identity = 359/610 (58.85%), Postives = 449/610 (73.61%), Query Frame = 0

Query: 5   TQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTW-------- 64
           + TP L + +P     DTQ  +N  +T     +K  PS + S  K  PN++         
Sbjct: 14  SDTPPLRVAVP-----DTQPLSNPPRTP----MKKTPSSTPSRSKPSPNRSTGKKDSPTV 73

Query: 65  --------------LDNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERF----- 124
                         LDNPD GP+LLKLARD I SGE P KALDYA+RA KSFER      
Sbjct: 74  SSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSFERCCAAVA 133

Query: 125 -PSLG-------VELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYS 184
            P  G       ++L MSLHV+AAI+C LG+FDEA+P LE++I+V +P  GPDH+LA +S
Sbjct: 134 PPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHSLAAFS 193

Query: 185 GYMQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKA 244
           G+MQLGDT SMLGQ+DRSI+CYE+GL IQI    D+DP+V ETCRYLAEA VQAMQF KA
Sbjct: 194 GHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEAYVQAMQFNKA 253

Query: 245 KKYCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDT 304
           ++ CKKTLEIH+ HS PAS +EA DRRLMA+ICEA GD E+ALEHLVLASMAM+A G ++
Sbjct: 254 EELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLVLASMAMIASGQES 313

Query: 305 EVAAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSK 364
           EVA+ID SIG+IY SLCRFDEA+F+YQKALT+F ++KG++H +VAS+F+RLAELY+RT K
Sbjct: 314 EVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGK 373

Query: 365 SREAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILER 424
            RE+KSY +NALRIY KP  G + EEI+ GL EISA+YE+ +EPEEA  +LQ++ K+LE 
Sbjct: 374 LRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKSMKLLED 433

Query: 425 GGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQ 484
             G        A+AG+EA+MGVM+Y VG+Y +AR AFE A+ KLR  G KSA FGV+LNQ
Sbjct: 434 KPG-----QQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAAGEKSAFFGVVLNQ 493

Query: 485 MGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEIL 544
           MGLAC+QL+KI EA +LF+EA+ +LE++ G    DTL V SNLAA YDAMGR+E+AIEIL
Sbjct: 494 MGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIEDAIEIL 553

Query: 545 EDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKE-VA 578
           E +LK+REE LGTANP+ ++EK+RL ELLKEAGR+RN KAKSLQ L+  N+   KKE  A
Sbjct: 554 EQVLKLREEKLGTANPDFEDEKKRLAELLKEAGRSRNYKAKSLQNLIDPNARPPKKESSA 609

BLAST of Lsi09G016730 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.8e-146
Identity = 282/523 (53.92%), Postives = 375/523 (71.70%), Query Frame = 0

Query: 44  SQSPQKLGPNQTWLDNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERF----PS 103
           S+    LG      ++P+ G  LLK AR+ + SGE+  KALD A+RA K FE+       
Sbjct: 135 SRKSPDLGKVSVDEESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQ 194

Query: 104 LGVELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYS 163
           LG+ L MSLH++AAI+  LG++++A+PVLE+SIE+    +G DHALAK++G MQLGD Y 
Sbjct: 195 LGLNLVMSLHILAAIYAGLGRYNDAVPVLERSIEIPMIEDGEDHALAKFAGCMQLGDMYG 254

Query: 164 MLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEI 223
           ++GQ++ SI  Y  GL IQ     +SD +V ETCRYLAEA+VQAMQFE+A + C+  L+I
Sbjct: 255 LMGQVENSIMLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDI 314

Query: 224 HKQH--SPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDAS 283
           HK++  +  AS +EA DR+LM LIC+A GD E ALEH VLASMAM +  H  +VAA+D S
Sbjct: 315 HKENGAAATASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCS 374

Query: 284 IGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYA 343
           IGD Y SL RFDEAIFAYQKAL +F   KG++H SVA +++RLA+LYN+  K+R++KSY 
Sbjct: 375 IGDAYMSLARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYC 434

Query: 344 DNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGS 403
           +NAL+IYLKP  G   EE+++G +EISA+Y++ NE ++A  +L+RA KI     G     
Sbjct: 435 ENALKIYLKPTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPG----- 494

Query: 404 GSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGG-AKSAMFGVLLNQMGLACIQ 463
               +AGIEAQMGV+ YM+G Y E+   F+ AI K R  G  K+A+FG+ LNQMGLAC+Q
Sbjct: 495 QQNTIAGIEAQMGVVTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQ 554

Query: 464 LYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVR 523
            Y I EA  LF+EAK +LE++ G YH DTLAV SNLA  YDAMGR+++AIEILE ++  R
Sbjct: 555 RYAINEAADLFEEAKTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTR 614

Query: 524 EEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSN 560
           EE LGTANPEV++EK+RL  LLKEAGR R+K+ ++L  LL +N
Sbjct: 615 EEKLGTANPEVEDEKQRLAALLKEAGRGRSKRNRALLTLLDNN 652

BLAST of Lsi09G016730 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 3.0e-142
Identity = 277/506 (54.74%), Postives = 365/506 (72.13%), Query Frame = 0

Query: 58  DNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLG---VELPMSLHVVAAI 117
           +N +   +LL  AR+ + SG+S  KAL+   RAAK FE     G   +E  M LHV AA+
Sbjct: 142 ENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHVTAAV 201

Query: 118 HCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDG 177
           HC+L +++EAIPVL++S+E+     G +HALAK++G MQLGDTY+M+GQL+ SISCY +G
Sbjct: 202 HCKLKEYNEAIPVLQRSVEIPVVEEGEEHALAKFAGLMQLGDTYAMVGQLESSISCYTEG 261

Query: 178 LMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATD 237
           L IQ     ++DP+V ETCRYLAEA VQA++F++A++ C+  L IH++   P S  EA D
Sbjct: 262 LNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGLPGSIAEAAD 321

Query: 238 RRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFA 297
           RRLM LICE  GD E+ALEHLVLASMAM A+G ++EVA +D SIGD Y SL RFDEAI A
Sbjct: 322 RRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSLSRFDEAICA 381

Query: 298 YQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCE 357
           YQK+LT   + KG++H +V S++IRLA+LYNRT K REAKSY +NALRIY    +  S E
Sbjct: 382 YQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYESHNLEISPE 441

Query: 358 EISSGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFY 417
           EI+SGL +IS + E+ NE E+A  +LQ+A KI     G         +AGIEAQMGV++Y
Sbjct: 442 EIASGLTDISVICESMNEVEQAITLLQKALKIYADSPG-----QKIMIAGIEAQMGVLYY 501

Query: 418 MVGKYGEARKAFEGAIGKLRGGGAK-SAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLV 477
           M+GKY E+   F+ AI KLR  G K S  FG+ LNQMGLACIQL  I EA++LF+EAK +
Sbjct: 502 MMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEEAKCI 561

Query: 478 LEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRR 537
           LE++ G YH +TL + SNLA AYDA+GR+++AI++L  ++ VREE LGTANP  ++EKRR
Sbjct: 562 LEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTEDEKRR 621

Query: 538 LEELLKEAGRARNKKAKSLQILLGSN 560
           L +LLKEAG    +KAKSL+ L+ S+
Sbjct: 622 LAQLLKEAGNVTGRKAKSLKTLIDSD 642

BLAST of Lsi09G016730 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.4e-06
Identity = 80/366 (21.86%), Postives = 151/366 (41.26%), Query Frame = 0

Query: 203  AMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRL--MALICEALGDSESALEHLVLASM 262
            A ++  A K  +K  E  ++ +  A   E   R L  + L+ +A+   + +LE    A  
Sbjct: 892  ASEYFDALKQYEKNCEGEEKMTSLADLYETLGRFLKDLGLLSQAVTPLQRSLEIRETA-- 951

Query: 263  AMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRL 322
              L   H +   ++    G +Y    +F  A   Y++AL I  +  G  HL VA     L
Sbjct: 952  --LDPDHPSVAQSLHQLAG-VYMQSKKFGNAEQLYKQALEISENAYGSEHLRVARELDAL 1011

Query: 323  AELYNRTSKSREAKSYADNALRIYLKP-----------------------PMGASCEEIS 382
            A LY + +K  +A+     +L+I  K                         +G    + +
Sbjct: 1012 AVLYQKQNKFEQAEQLRKKSLKIRQKSARRKGSMYGFALLRRRALQLEELTLGKDTSDNA 1071

Query: 383  SGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVG 442
              L E+  +Y   N  E A   L+R+ ++ ER  G      ++++  + A    ++    
Sbjct: 1072 RTLNELGVLYYLQNNLETAETFLKRSLEMRERVLGADHPDCAQSINNLAA----LYNEKK 1131

Query: 443  KYGEARKAFEGAIG-KLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEE 502
            +Y +A + +E A+  + R            +  + +   +  K+ +A+ L++ A  + ++
Sbjct: 1132 QYDKAEELYERALDIRRRALSPDHPSLAYTVKHLAVLYKRKGKLDKAVPLYELAVDIRQK 1191

Query: 503  QYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEE 543
             +G  H        NLA  Y  M + ++A+ + E  +K+ E+ LG  +P V E  + L  
Sbjct: 1192 SFGPKHPSVATALVNLAVLYCQMKKQDDALPLYERAMKIYEDSLGRMHPRVGETLKNLAV 1248

BLAST of Lsi09G016730 vs. ExPASy Swiss-Prot
Match: P0CI65 (Nephrocystin-3 OS=Danio rerio OX=7955 GN=nphp3 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.1e-06
Identity = 50/176 (28.41%), Postives = 82/176 (46.59%), Query Frame = 0

Query: 50   LGPNQTWLDNPDHGPYLLKLA------RDTIISGESPTKALDYAVRAAKSFERFPSLGVE 109
            LGP     D+PD    L  LA      ++   + E   +ALD   RA       PSL   
Sbjct: 1105 LGP-----DHPDCAQSLNNLAALHSERKEYESAEELYERALDIRKRALA--PDHPSLA-- 1164

Query: 110  LPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQ 169
               +L  +A ++ R G+ ++A+P+ E ++E+ E   GP H  +  +  + L   Y  L Q
Sbjct: 1165 --YTLKHLAMLYKRRGKLEKAVPLYELALEIREKSFGPKHP-SVATALVNLAVLYCQLKQ 1224

Query: 170  LDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEI 220
               ++  YE  L +  ++     P+V ET + LA  + +   FEKA +  K+ +EI
Sbjct: 1225 HSDALPLYERALKVYEDSLGRLHPRVGETLKNLAVLSYEEGDFEKAAELYKRAMEI 1268

BLAST of Lsi09G016730 vs. ExPASy TrEMBL
Match: E5GCM7 (Kinesin light chain OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.7e-269
Identity = 492/549 (89.62%), Postives = 515/549 (93.81%), Query Frame = 0

Query: 32  MTPPWLKPIPSPSQSPQKLGPNQTWLDNPDHGPYLLKLARDTIISGESPTKALDYAVRAA 91
           MTPP  K IPSPS S Q L   QTWLDNPDHGPYLLKLARDTI+S ESPTKALDYA+RAA
Sbjct: 1   MTPPCPKLIPSPSPSTQSLELKQTWLDNPDHGPYLLKLARDTIVSDESPTKALDYAIRAA 60

Query: 92  KSFERFPSLGVELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGY 151
           KSFERFP  GVELPMSLH+VAAIHCRLGQFD AIPVLE++IEV EPGNG DHALAKYSGY
Sbjct: 61  KSFERFPGTGVELPMSLHMVAAIHCRLGQFDVAIPVLERAIEVVEPGNGLDHALAKYSGY 120

Query: 152 MQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKK 211
           MQLGDTYSMLGQLD+SISCYE GLMIQI+AFTDSDP+VAETCRYLAEA+VQAMQFEKAKK
Sbjct: 121 MQLGDTYSMLGQLDQSISCYEAGLMIQIHAFTDSDPKVAETCRYLAEAHVQAMQFEKAKK 180

Query: 212 YCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEV 271
           YCKKTL+IHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAM+AHGHD EV
Sbjct: 181 YCKKTLDIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMIAHGHDAEV 240

Query: 272 AAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSR 331
           AAID SIGDIY SLCRFDEAIFAYQKALTIFNSTKG+SHLSVASLF+RLAELYNRT KSR
Sbjct: 241 AAIDVSIGDIYASLCRFDEAIFAYQKALTIFNSTKGESHLSVASLFVRLAELYNRTGKSR 300

Query: 332 EAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGG 391
           EAKSYADNALRIYLKP  GAS EEISSGLVEISAVYEAANEPEEAF VLQRA  ILERGG
Sbjct: 301 EAKSYADNALRIYLKPQAGASYEEISSGLVEISAVYEAANEPEEAFKVLQRATTILERGG 360

Query: 392 G--GGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQ 451
           G  GGR SGS ++AGIEAQMGVMFYMVGKYGEARKAFEGAI KLR  GAKSAMFG+LLNQ
Sbjct: 361 GGEGGRRSGSGSMAGIEAQMGVMFYMVGKYGEARKAFEGAIEKLR--GAKSAMFGILLNQ 420

Query: 452 MGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEIL 511
           MGLACIQLYKIGEA++LFQEAK+VLEEQYGVYHSDTLAVSSNLAAAYDAMG VEEAIEIL
Sbjct: 421 MGLACIQLYKIGEAVRLFQEAKVVLEEQYGVYHSDTLAVSSNLAAAYDAMGSVEEAIEIL 480

Query: 512 EDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKEVAK 571
           E+ILKVREEMLGTANPEVDEE+RRLEELLKEAGRARNKKAKSLQILLGSNSLR+KKEVAK
Sbjct: 481 EEILKVREEMLGTANPEVDEERRRLEELLKEAGRARNKKAKSLQILLGSNSLRMKKEVAK 540

Query: 572 RWSGGFSFR 579
           RW+GGFSFR
Sbjct: 541 RWTGGFSFR 547

BLAST of Lsi09G016730 vs. ExPASy TrEMBL
Match: A0A1S3CB06 (kinesin light chain 1-like OS=Cucumis melo OX=3656 GN=LOC103498798 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.7e-269
Identity = 492/549 (89.62%), Postives = 515/549 (93.81%), Query Frame = 0

Query: 32  MTPPWLKPIPSPSQSPQKLGPNQTWLDNPDHGPYLLKLARDTIISGESPTKALDYAVRAA 91
           MTPP  K IPSPS S Q L   QTWLDNPDHGPYLLKLARDTI+S ESPTKALDYA+RAA
Sbjct: 1   MTPPCPKLIPSPSPSTQSLELKQTWLDNPDHGPYLLKLARDTIVSDESPTKALDYAIRAA 60

Query: 92  KSFERFPSLGVELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGY 151
           KSFERFP  GVELPMSLH+VAAIHCRLGQFD AIPVLE++IEV EPGNG DHALAKYSGY
Sbjct: 61  KSFERFPGTGVELPMSLHMVAAIHCRLGQFDVAIPVLERAIEVVEPGNGLDHALAKYSGY 120

Query: 152 MQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKK 211
           MQLGDTYSMLGQLD+SISCYE GLMIQI+AFTDSDP+VAETCRYLAEA+VQAMQFEKAKK
Sbjct: 121 MQLGDTYSMLGQLDQSISCYEAGLMIQIHAFTDSDPKVAETCRYLAEAHVQAMQFEKAKK 180

Query: 212 YCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEV 271
           YCKKTL+IHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAM+AHGHD EV
Sbjct: 181 YCKKTLDIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMIAHGHDAEV 240

Query: 272 AAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSR 331
           AAID SIGDIY SLCRFDEAIFAYQKALTIFNSTKG+SHLSVASLF+RLAELYNRT KSR
Sbjct: 241 AAIDVSIGDIYASLCRFDEAIFAYQKALTIFNSTKGESHLSVASLFVRLAELYNRTGKSR 300

Query: 332 EAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGG 391
           EAKSYADNALRIYLKP  GAS EEISSGLVEISAVYEAANEPEEAF VLQRA  ILERGG
Sbjct: 301 EAKSYADNALRIYLKPQAGASYEEISSGLVEISAVYEAANEPEEAFKVLQRATTILERGG 360

Query: 392 G--GGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQ 451
           G  GGR SGS ++AGIEAQMGVMFYMVGKYGEARKAFEGAI KLR  GAKSAMFG+LLNQ
Sbjct: 361 GGEGGRRSGSGSMAGIEAQMGVMFYMVGKYGEARKAFEGAIEKLR--GAKSAMFGILLNQ 420

Query: 452 MGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEIL 511
           MGLACIQLYKIGEA++LFQEAK+VLEEQYGVYHSDTLAVSSNLAAAYDAMG VEEAIEIL
Sbjct: 421 MGLACIQLYKIGEAVRLFQEAKVVLEEQYGVYHSDTLAVSSNLAAAYDAMGSVEEAIEIL 480

Query: 512 EDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKEVAK 571
           E+ILKVREEMLGTANPEVDEE+RRLEELLKEAGRARNKKAKSLQILLGSNSLR+KKEVAK
Sbjct: 481 EEILKVREEMLGTANPEVDEERRRLEELLKEAGRARNKKAKSLQILLGSNSLRMKKEVAK 540

Query: 572 RWSGGFSFR 579
           RW+GGFSFR
Sbjct: 541 RWTGGFSFR 547

BLAST of Lsi09G016730 vs. ExPASy TrEMBL
Match: A0A6J1H7R7 (protein KINESIN LIGHT CHAIN-RELATED 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460337 PE=4 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 6.5e-241
Identity = 447/561 (79.68%), Postives = 487/561 (86.81%), Query Frame = 0

Query: 3   IETQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTWLDNPDH 62
           I+  TPHL  Q+PPI++D        +KT T                      WLDNPDH
Sbjct: 12  IKMATPHLRTQVPPIDDD--------HKTTT--------------------SRWLDNPDH 71

Query: 63  GPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLGVELPMSLHVVAAIHCRLGQFD 122
           GPYLLKLARDTIISGESPTKALDYA RAAKSFERFPS GVELPMSLH+VAAI+CRLGQFD
Sbjct: 72  GPYLLKLARDTIISGESPTKALDYAFRAAKSFERFPSPGVELPMSLHMVAAIYCRLGQFD 131

Query: 123 EAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMIQINAF 182
           +AIPVLE+SIEV E  N PDHALAKYSGYMQLGDTYSMLGQLDRSISCYE GLMIQ +A 
Sbjct: 132 QAIPVLERSIEVVERRNEPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEAGLMIQTHAL 191

Query: 183 TDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRLMALIC 242
            DSDP+VAETCRYLAEA+VQAMQFE+AKKYCKKTL+IHKQHSPPASPQEATDRRLMALIC
Sbjct: 192 PDSDPKVAETCRYLAEAHVQAMQFEEAKKYCKKTLDIHKQHSPPASPQEATDRRLMALIC 251

Query: 243 EALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKALTIF 302
           EALGDSESALEHLVLASMAM+AHGHD EVAAID SIGDIYT+LCRFDEAIFAYQKALTIF
Sbjct: 252 EALGDSESALEHLVLASMAMIAHGHDAEVAAIDVSIGDIYTTLCRFDEAIFAYQKALTIF 311

Query: 303 NSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEISSGLVE 362
           NSTKGDSHLSVASLFIRLAELYNRT K+REAKSYADNALRIYLKPP G++ EE+SSGLVE
Sbjct: 312 NSTKGDSHLSVASLFIRLAELYNRTGKTREAKSYADNALRIYLKPPEGSTDEEVSSGLVE 371

Query: 363 ISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEA 422
           I+AVYEA NE EEAFGVL+RA K++ERGGGG      R  AGIEAQMGVMFY++GKYGEA
Sbjct: 372 IAAVYEAGNEAEEAFGVLRRAVKVVERGGGG------RVAAGIEAQMGVMFYVMGKYGEA 431

Query: 423 RKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYH 482
           R+ FE AIG++R GG K A+FGV+LNQMGLAC+QLYKI EA+++FQEA++VL E YGVYH
Sbjct: 432 RRVFERAIGRVR-GGKKGALFGVVLNQMGLACVQLYKIEEAVRVFQEARVVLVEHYGVYH 491

Query: 483 SDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEELLKEAG 542
           SDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEE+RRLE+LLKEAG
Sbjct: 492 SDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEERRRLEQLLKEAG 537

Query: 543 RARNKKAKSLQILLGSNSLRI 564
           RA N KAKSLQ LL S SLR+
Sbjct: 552 RAPNNKAKSLQNLLASQSLRM 537

BLAST of Lsi09G016730 vs. ExPASy TrEMBL
Match: A0A0A0KCI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106760 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.9e-233
Identity = 434/477 (90.99%), Postives = 452/477 (94.76%), Query Frame = 0

Query: 106 MSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLD 165
           MSLHVVAAIHCRLGQFD AIPVLE+SIE  EPGNG DHALAKYSGYMQLGDTYSMLGQLD
Sbjct: 1   MSLHVVAAIHCRLGQFDVAIPVLERSIEAVEPGNGLDHALAKYSGYMQLGDTYSMLGQLD 60

Query: 166 RSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSP 225
           +SISCYE GLMIQINAFTDSDP+VAETCRYLAEA+VQAMQFEKAKKYCKKTL+IHKQHSP
Sbjct: 61  QSISCYEAGLMIQINAFTDSDPRVAETCRYLAEAHVQAMQFEKAKKYCKKTLDIHKQHSP 120

Query: 226 PASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSL 285
           PASP+EATDR LMALICEALGDSESALEHLVLASMAM+AHGHD EVAAIDASIGDIY SL
Sbjct: 121 PASPKEATDRHLMALICEALGDSESALEHLVLASMAMIAHGHDAEVAAIDASIGDIYASL 180

Query: 286 CRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYL 345
           CRFDEAIFAYQKALTIFNSTKG+SHLSVASLF+RLAELYNRT KSREAKSYADNALRIYL
Sbjct: 181 CRFDEAIFAYQKALTIFNSTKGESHLSVASLFVRLAELYNRTGKSREAKSYADNALRIYL 240

Query: 346 KPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERG--GGGGR--GSGSRA 405
           KP +GAS EEISSGLVEISAVYEA NEPEEAF VLQRA  ILE+G  GGGGR  GSGS A
Sbjct: 241 KPQVGASYEEISSGLVEISAVYEATNEPEEAFKVLQRATTILEQGGRGGGGRRSGSGSGA 300

Query: 406 VAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIG 465
           +AGIEAQMGVMFYMVGKYGEARKAFEGAIGKLR  G KSAMFGVLLNQMGL CIQLYKIG
Sbjct: 301 MAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLR--GTKSAMFGVLLNQMGLTCIQLYKIG 360

Query: 466 EAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLG 525
           EA++LFQEAK+VLEE YGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILE+ILKVREEMLG
Sbjct: 361 EAVRLFQEAKVVLEEHYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEEILKVREEMLG 420

Query: 526 TANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKEVAKRWSGGFSFR 579
           TANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLR+KKEVAKRW+GGFSFR
Sbjct: 421 TANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRMKKEVAKRWTGGFSFR 475

BLAST of Lsi09G016730 vs. ExPASy TrEMBL
Match: A0A6J1DAT9 (protein KINESIN LIGHT CHAIN-RELATED 1-like OS=Momordica charantia OX=3673 GN=LOC111018972 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 1.1e-203
Identity = 384/476 (80.67%), Postives = 421/476 (88.45%), Query Frame = 0

Query: 106 MSLHVVAAIHCRLGQFDEAIPVLEQSIEVA-EPGNGPDHALAKYSGYMQLGDTYSMLGQL 165
           MSLHVVAAI+CRLG+ +EA+PVL++SIEV  E  NG +HA+AKYSGYMQLGDTYSMLGQL
Sbjct: 1   MSLHVVAAIYCRLGRLEEAVPVLKRSIEVVREVENGLEHAVAKYSGYMQLGDTYSMLGQL 60

Query: 166 DRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHS 225
           D SI+CYE GLMIQI+ FTDSDP+VA+TCRYLAEA+VQAMQF +AKKYCKKTLEIHKQHS
Sbjct: 61  DCSIACYEAGLMIQIHTFTDSDPKVAQTCRYLAEAHVQAMQFNEAKKYCKKTLEIHKQHS 120

Query: 226 PPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTS 285
            P+SP+EATDRRLMALICEALGD ESALEHLVLASMAM+AHGHD EVAAID SIGDIY S
Sbjct: 121 APSSPEEATDRRLMALICEALGDHESALEHLVLASMAMIAHGHDAEVAAIDVSIGDIYFS 180

Query: 286 LCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIY 345
           LCRFDEAIFAYQKAL +F ST+G+SHLSVASLFIRLAELYN+T KSREAKSYADNALRIY
Sbjct: 181 LCRFDEAIFAYQKALIVFKSTRGESHLSVASLFIRLAELYNKTGKSREAKSYADNALRIY 240

Query: 346 LKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAG 405
            +P +GASCEE+SSGLVEISAVYEA  EPEEAF VLQRA K +E GGGG       A+AG
Sbjct: 241 TRPGIGASCEELSSGLVEISAVYEAVGEPEEAFRVLQRAMKAVEEGGGGQHS----AIAG 300

Query: 406 IEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAI 465
           IEA+MGVM YMVG+YGEARKAFEGAIGKLRG G KS +FG+L+NQMGLACIQLY+I EA 
Sbjct: 301 IEAEMGVMLYMVGRYGEARKAFEGAIGKLRGAG-KSVLFGILVNQMGLACIQLYRIEEAA 360

Query: 466 KLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTAN 525
           KLF+EAKLVLE++YG YHS TL V SNLAAA+DAMGRV EAIEILE ILKVREEMLGTAN
Sbjct: 361 KLFEEAKLVLEQEYGTYHSYTLGVCSNLAAAFDAMGRVGEAIEILEHILKVREEMLGTAN 420

Query: 526 PEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRI-KKEVAKRWSGGFSFRA 580
           PEVDEEKRRLEELLKEAGRARNKKA SLQ LL SNSL I KK+V   W GGFSF A
Sbjct: 421 PEVDEEKRRLEELLKEAGRARNKKANSLQNLLASNSLGIMKKQVTNTWFGGFSFTA 471

BLAST of Lsi09G016730 vs. NCBI nr
Match: XP_038906715.1 (protein KINESIN LIGHT CHAIN-RELATED 1-like [Benincasa hispida] >XP_038906716.1 protein KINESIN LIGHT CHAIN-RELATED 1-like [Benincasa hispida])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.3e-295
Identity = 533/579 (92.06%), Postives = 549/579 (94.82%), Query Frame = 0

Query: 1   MAIETQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTWLDNP 60
           M IE +TPHL IQ+PPI NDDTQHQNNNYKTMTPPWLK IPSPS SPQK  PN+TWLDNP
Sbjct: 1   MGIEPKTPHLRIQVPPIVNDDTQHQNNNYKTMTPPWLKLIPSPSPSPQKSEPNRTWLDNP 60

Query: 61  DHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLGVELPMSLHVVAAIHCRLGQ 120
           DHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFP LGVELPMSLHVVAAIHCRLGQ
Sbjct: 61  DHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPGLGVELPMSLHVVAAIHCRLGQ 120

Query: 121 FDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMIQIN 180
           FDEAI VLE+SI+VAE GNG DHALAKYSGYMQLGDTYSMLGQLDRSISCYE GLMIQIN
Sbjct: 121 FDEAISVLERSIDVAEAGNGLDHALAKYSGYMQLGDTYSMLGQLDRSISCYEAGLMIQIN 180

Query: 181 AFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRLMAL 240
           AFTDSDPQVAETCRYLAEA+VQAMQFEKAKKYCKKTL+IHKQHSPPASPQEATDRRLMAL
Sbjct: 181 AFTDSDPQVAETCRYLAEAHVQAMQFEKAKKYCKKTLDIHKQHSPPASPQEATDRRLMAL 240

Query: 241 ICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKALT 300
           ICEALGDSESALEHLVLASMAM+AHGHDTEVAAIDASIGDIY SLCRFDEAIFAYQKALT
Sbjct: 241 ICEALGDSESALEHLVLASMAMIAHGHDTEVAAIDASIGDIYASLCRFDEAIFAYQKALT 300

Query: 301 IFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEISSGL 360
           IF STKG+SHLSVASLFIRLAELYNRT KSREAKSY DNALRIYLKPP GASCEE+SSGL
Sbjct: 301 IFKSTKGESHLSVASLFIRLAELYNRTGKSREAKSYVDNALRIYLKPPSGASCEELSSGL 360

Query: 361 VEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVGKYG 420
           VEIS+VYEA NEPEEAF VLQRA  +LE+G GGGR SGS AVAGIEAQMGVMFYMVGKYG
Sbjct: 361 VEISSVYEATNEPEEAFRVLQRATMVLEQGVGGGRWSGSGAVAGIEAQMGVMFYMVGKYG 420

Query: 421 EARKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEEQYGV 480
           EARKAFEGAIGKLR  GAKSAMFGVLLNQMGLACIQLYKIGEA+KLFQEAK+VLEEQYGV
Sbjct: 421 EARKAFEGAIGKLR-AGAKSAMFGVLLNQMGLACIQLYKIGEAVKLFQEAKVVLEEQYGV 480

Query: 481 YHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEELLKE 540
           YHSDTLAVSSNLAAAYDAMG VEEAIEILE ILKVREEMLGTANPEVDEEKRRLEELLKE
Sbjct: 481 YHSDTLAVSSNLAAAYDAMGSVEEAIEILEHILKVREEMLGTANPEVDEEKRRLEELLKE 540

Query: 541 AGRARNKKAKSLQILLGSNSLRIKKEVAKRWSGGFSFRA 580
           AGRARNKKAKSLQILLGSN LR+KKEVAKRWSGGFSFRA
Sbjct: 541 AGRARNKKAKSLQILLGSNPLRMKKEVAKRWSGGFSFRA 578

BLAST of Lsi09G016730 vs. NCBI nr
Match: XP_004140507.3 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucumis sativus] >KAE8646783.1 hypothetical protein Csa_005253 [Cucumis sativus])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.5e-289
Identity = 528/584 (90.41%), Postives = 550/584 (94.18%), Query Frame = 0

Query: 1   MAIETQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPI--PSPSQSPQKLGPNQTWLD 60
           M +ETQTPHLHIQ+PPI NDDTQHQNNN+KTMTPP LK I  PSPS SPQ L PN+TWLD
Sbjct: 1   MGMETQTPHLHIQVPPIGNDDTQHQNNNFKTMTPPCLKLISSPSPSPSPQSLEPNRTWLD 60

Query: 61  NPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLGVELPMSLHVVAAIHCRL 120
           NPDHGPYLLKLARDTIIS E+PTKALDYAVRAAKSFERFPS GVELPMSLHVVAAIHCRL
Sbjct: 61  NPDHGPYLLKLARDTIISSENPTKALDYAVRAAKSFERFPSTGVELPMSLHVVAAIHCRL 120

Query: 121 GQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMIQ 180
           GQFD AIPVLE+SIE  EPGNG DHALAKYSGYMQLGDTYSMLGQLD+SISCYE GLMIQ
Sbjct: 121 GQFDVAIPVLERSIEAVEPGNGLDHALAKYSGYMQLGDTYSMLGQLDQSISCYEAGLMIQ 180

Query: 181 INAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRLM 240
           INAFTDSDP+VAETCRYLAEA+VQAMQFEKAKKYCKKTL+IHKQHSPPASPQEATDRRLM
Sbjct: 181 INAFTDSDPRVAETCRYLAEAHVQAMQFEKAKKYCKKTLDIHKQHSPPASPQEATDRRLM 240

Query: 241 ALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKA 300
           ALICEALGDSESALEHLVLASMAM+AHGHD EVAAIDASIGDIY SLCRFDEAIFAYQKA
Sbjct: 241 ALICEALGDSESALEHLVLASMAMIAHGHDAEVAAIDASIGDIYASLCRFDEAIFAYQKA 300

Query: 301 LTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEISS 360
           LTIFNSTKG+SHLSVASLF+RLAELYNRT KSREAKSYADNALRIYLKP +GAS EEISS
Sbjct: 301 LTIFNSTKGESHLSVASLFVRLAELYNRTGKSREAKSYADNALRIYLKPQVGASYEEISS 360

Query: 361 GLVEISAVYEAANEPEEAFGVLQRARKILERG--GGGGR--GSGSRAVAGIEAQMGVMFY 420
           GLVEISAVYEA NEPEEAF VLQRA  ILE+G  GGGGR  GSGS A+AGIEAQMGVMFY
Sbjct: 361 GLVEISAVYEATNEPEEAFKVLQRATTILEQGGRGGGGRRSGSGSGAMAGIEAQMGVMFY 420

Query: 421 MVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVL 480
           MVGKYGEARKAFEGAIGKLR  G KSAMFGVLLNQMGLACIQLYKIGEA++LFQEAK+VL
Sbjct: 421 MVGKYGEARKAFEGAIGKLR--GTKSAMFGVLLNQMGLACIQLYKIGEAVRLFQEAKVVL 480

Query: 481 EEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRL 540
           EE YGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILE+ILKVREEMLGTANPEVDEEKRRL
Sbjct: 481 EEHYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEEILKVREEMLGTANPEVDEEKRRL 540

Query: 541 EELLKEAGRARNKKAKSLQILLGSNSLRIKKEVAKRWSGGFSFR 579
           EELLKEAGRARNKKAKSLQILLGSNSLR+KKEVAKRW+GGFSFR
Sbjct: 541 EELLKEAGRARNKKAKSLQILLGSNSLRMKKEVAKRWTGGFSFR 582

BLAST of Lsi09G016730 vs. NCBI nr
Match: XP_008459760.1 (PREDICTED: kinesin light chain 1-like [Cucumis melo] >ADN34226.1 kinesin light chain [Cucumis melo subsp. melo])

HSP 1 Score: 935.6 bits (2417), Expect = 2.0e-268
Identity = 492/549 (89.62%), Postives = 515/549 (93.81%), Query Frame = 0

Query: 32  MTPPWLKPIPSPSQSPQKLGPNQTWLDNPDHGPYLLKLARDTIISGESPTKALDYAVRAA 91
           MTPP  K IPSPS S Q L   QTWLDNPDHGPYLLKLARDTI+S ESPTKALDYA+RAA
Sbjct: 1   MTPPCPKLIPSPSPSTQSLELKQTWLDNPDHGPYLLKLARDTIVSDESPTKALDYAIRAA 60

Query: 92  KSFERFPSLGVELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGY 151
           KSFERFP  GVELPMSLH+VAAIHCRLGQFD AIPVLE++IEV EPGNG DHALAKYSGY
Sbjct: 61  KSFERFPGTGVELPMSLHMVAAIHCRLGQFDVAIPVLERAIEVVEPGNGLDHALAKYSGY 120

Query: 152 MQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKK 211
           MQLGDTYSMLGQLD+SISCYE GLMIQI+AFTDSDP+VAETCRYLAEA+VQAMQFEKAKK
Sbjct: 121 MQLGDTYSMLGQLDQSISCYEAGLMIQIHAFTDSDPKVAETCRYLAEAHVQAMQFEKAKK 180

Query: 212 YCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEV 271
           YCKKTL+IHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAM+AHGHD EV
Sbjct: 181 YCKKTLDIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMIAHGHDAEV 240

Query: 272 AAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSR 331
           AAID SIGDIY SLCRFDEAIFAYQKALTIFNSTKG+SHLSVASLF+RLAELYNRT KSR
Sbjct: 241 AAIDVSIGDIYASLCRFDEAIFAYQKALTIFNSTKGESHLSVASLFVRLAELYNRTGKSR 300

Query: 332 EAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGG 391
           EAKSYADNALRIYLKP  GAS EEISSGLVEISAVYEAANEPEEAF VLQRA  ILERGG
Sbjct: 301 EAKSYADNALRIYLKPQAGASYEEISSGLVEISAVYEAANEPEEAFKVLQRATTILERGG 360

Query: 392 G--GGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQ 451
           G  GGR SGS ++AGIEAQMGVMFYMVGKYGEARKAFEGAI KLR  GAKSAMFG+LLNQ
Sbjct: 361 GGEGGRRSGSGSMAGIEAQMGVMFYMVGKYGEARKAFEGAIEKLR--GAKSAMFGILLNQ 420

Query: 452 MGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEIL 511
           MGLACIQLYKIGEA++LFQEAK+VLEEQYGVYHSDTLAVSSNLAAAYDAMG VEEAIEIL
Sbjct: 421 MGLACIQLYKIGEAVRLFQEAKVVLEEQYGVYHSDTLAVSSNLAAAYDAMGSVEEAIEIL 480

Query: 512 EDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKEVAK 571
           E+ILKVREEMLGTANPEVDEE+RRLEELLKEAGRARNKKAKSLQILLGSNSLR+KKEVAK
Sbjct: 481 EEILKVREEMLGTANPEVDEERRRLEELLKEAGRARNKKAKSLQILLGSNSLRMKKEVAK 540

Query: 572 RWSGGFSFR 579
           RW+GGFSFR
Sbjct: 541 RWTGGFSFR 547

BLAST of Lsi09G016730 vs. NCBI nr
Match: KAG6575259.1 (Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 864.4 bits (2232), Expect = 5.6e-247
Identity = 463/575 (80.52%), Postives = 502/575 (87.30%), Query Frame = 0

Query: 7   TPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTWLDNPDHGPYL 66
           TPHL IQ+PPI++D        +KT T                      WLDNPDHGPYL
Sbjct: 3   TPHLRIQVPPIDDD--------HKTTT--------------------SQWLDNPDHGPYL 62

Query: 67  LKLARDTIISGESPTKALDYAVRAAKSFERFPSLGVELPMSLHVVAAIHCRLGQFDEAIP 126
           LKLARDTIISGESPTKALDYAVRAAKSFERFPS GVELPMSLH+VAAI+CRLGQFDEAIP
Sbjct: 63  LKLARDTIISGESPTKALDYAVRAAKSFERFPSPGVELPMSLHMVAAIYCRLGQFDEAIP 122

Query: 127 VLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSD 186
           VLE+SI+V E  N PDHALAKYSGYMQLGDTYSMLGQLDRSISCYE GLMIQ +A +DSD
Sbjct: 123 VLERSIKVVERRNEPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEAGLMIQTHALSDSD 182

Query: 187 PQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRLMALICEALG 246
           P+VAETCRYLAEA+VQAMQFE+AKKYCKKTL+IHKQHSPPASPQEATDRRLMALICEALG
Sbjct: 183 PKVAETCRYLAEAHVQAMQFEEAKKYCKKTLDIHKQHSPPASPQEATDRRLMALICEALG 242

Query: 247 DSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTK 306
           DSESALEHLVLASMAM+AHGHD EVAAID SIG+IY +LCRFDEAIFAYQKALTIFNSTK
Sbjct: 243 DSESALEHLVLASMAMIAHGHDAEVAAIDVSIGNIYATLCRFDEAIFAYQKALTIFNSTK 302

Query: 307 GDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEISSGLVEISAV 366
           GDSHLSVASLFIRLAELYNRT K+REAKSYADNALRIYLKPP G++ EE+SSGLVEI+AV
Sbjct: 303 GDSHLSVASLFIRLAELYNRTGKTREAKSYADNALRIYLKPPEGSTDEEVSSGLVEIAAV 362

Query: 367 YEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAF 426
           YEA NE  EAFGVLQRA K++ERGGG     G  A AGIEAQMGVMFYM+GKYGEAR+ F
Sbjct: 363 YEAGNE-AEAFGVLQRAVKVVERGGG-----GRAAAAGIEAQMGVMFYMMGKYGEARRVF 422

Query: 427 EGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTL 486
           E AIG++R GG K A+FGV+LNQMGLAC+QLYKI EA+++FQEA++VL E YGVYHSDTL
Sbjct: 423 ERAIGRVR-GGKKGALFGVVLNQMGLACVQLYKIEEAVRVFQEARVVLVEHYGVYHSDTL 482

Query: 487 AVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARN 546
           AVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEE+RRLEELLKEAGRA N
Sbjct: 483 AVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEERRRLEELLKEAGRAPN 542

Query: 547 KKAKSLQILLGSNSLRIKKEVA--KRWSGGFSFRA 580
           KKAKSLQ LL S SLR+KK+VA  K WSGGFSFRA
Sbjct: 543 KKAKSLQNLLASQSLRMKKQVAATKIWSGGFSFRA 542

BLAST of Lsi09G016730 vs. NCBI nr
Match: XP_022959334.1 (protein KINESIN LIGHT CHAIN-RELATED 1-like [Cucurbita moschata])

HSP 1 Score: 843.2 bits (2177), Expect = 1.3e-240
Identity = 447/561 (79.68%), Postives = 487/561 (86.81%), Query Frame = 0

Query: 3   IETQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTWLDNPDH 62
           I+  TPHL  Q+PPI++D        +KT T                      WLDNPDH
Sbjct: 12  IKMATPHLRTQVPPIDDD--------HKTTT--------------------SRWLDNPDH 71

Query: 63  GPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLGVELPMSLHVVAAIHCRLGQFD 122
           GPYLLKLARDTIISGESPTKALDYA RAAKSFERFPS GVELPMSLH+VAAI+CRLGQFD
Sbjct: 72  GPYLLKLARDTIISGESPTKALDYAFRAAKSFERFPSPGVELPMSLHMVAAIYCRLGQFD 131

Query: 123 EAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMIQINAF 182
           +AIPVLE+SIEV E  N PDHALAKYSGYMQLGDTYSMLGQLDRSISCYE GLMIQ +A 
Sbjct: 132 QAIPVLERSIEVVERRNEPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEAGLMIQTHAL 191

Query: 183 TDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRLMALIC 242
            DSDP+VAETCRYLAEA+VQAMQFE+AKKYCKKTL+IHKQHSPPASPQEATDRRLMALIC
Sbjct: 192 PDSDPKVAETCRYLAEAHVQAMQFEEAKKYCKKTLDIHKQHSPPASPQEATDRRLMALIC 251

Query: 243 EALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQKALTIF 302
           EALGDSESALEHLVLASMAM+AHGHD EVAAID SIGDIYT+LCRFDEAIFAYQKALTIF
Sbjct: 252 EALGDSESALEHLVLASMAMIAHGHDAEVAAIDVSIGDIYTTLCRFDEAIFAYQKALTIF 311

Query: 303 NSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEISSGLVE 362
           NSTKGDSHLSVASLFIRLAELYNRT K+REAKSYADNALRIYLKPP G++ EE+SSGLVE
Sbjct: 312 NSTKGDSHLSVASLFIRLAELYNRTGKTREAKSYADNALRIYLKPPEGSTDEEVSSGLVE 371

Query: 363 ISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEA 422
           I+AVYEA NE EEAFGVL+RA K++ERGGGG      R  AGIEAQMGVMFY++GKYGEA
Sbjct: 372 IAAVYEAGNEAEEAFGVLRRAVKVVERGGGG------RVAAGIEAQMGVMFYVMGKYGEA 431

Query: 423 RKAFEGAIGKLRGGGAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYH 482
           R+ FE AIG++R GG K A+FGV+LNQMGLAC+QLYKI EA+++FQEA++VL E YGVYH
Sbjct: 432 RRVFERAIGRVR-GGKKGALFGVVLNQMGLACVQLYKIEEAVRVFQEARVVLVEHYGVYH 491

Query: 483 SDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEELLKEAG 542
           SDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEE+RRLE+LLKEAG
Sbjct: 492 SDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEERRRLEQLLKEAG 537

Query: 543 RARNKKAKSLQILLGSNSLRI 564
           RA N KAKSLQ LL S SLR+
Sbjct: 552 RAPNNKAKSLQNLLASQSLRM 537

BLAST of Lsi09G016730 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 646.7 bits (1667), Expect = 1.7e-185
Identity = 359/610 (58.85%), Postives = 449/610 (73.61%), Query Frame = 0

Query: 5   TQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTW-------- 64
           + TP L + +P     DTQ  +N  +T     +K  PS + S  K  PN++         
Sbjct: 14  SDTPPLRVAVP-----DTQPLSNPPRTP----MKKTPSSTPSRSKPSPNRSTGKKDSPTV 73

Query: 65  --------------LDNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERF----- 124
                         LDNPD GP+LLKLARD I SGE P KALDYA+RA KSFER      
Sbjct: 74  SSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSFERCCAAVA 133

Query: 125 -PSLG-------VELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYS 184
            P  G       ++L MSLHV+AAI+C LG+FDEA+P LE++I+V +P  GPDH+LA +S
Sbjct: 134 PPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHSLAAFS 193

Query: 185 GYMQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKA 244
           G+MQLGDT SMLGQ+DRSI+CYE+GL IQI    D+DP+V ETCRYLAEA VQAMQF KA
Sbjct: 194 GHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEAYVQAMQFNKA 253

Query: 245 KKYCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDT 304
           ++ CKKTLEIH+ HS PAS +EA DRRLMA+ICEA GD E+ALEHLVLASMAM+A G ++
Sbjct: 254 EELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLVLASMAMIASGQES 313

Query: 305 EVAAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSK 364
           EVA+ID SIG+IY SLCRFDEA+F+YQKALT+F ++KG++H +VAS+F+RLAELY+RT K
Sbjct: 314 EVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGK 373

Query: 365 SREAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILER 424
            RE+KSY +NALRIY KP  G + EEI+ GL EISA+YE+ +EPEEA  +LQ++ K+LE 
Sbjct: 374 LRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKSMKLLED 433

Query: 425 GGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQ 484
             G        A+AG+EA+MGVM+Y VG+Y +AR AFE A+ KLR  G KSA FGV+LNQ
Sbjct: 434 KPG-----QQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAAGEKSAFFGVVLNQ 493

Query: 485 MGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEIL 544
           MGLAC+QL+KI EA +LF+EA+ +LE++ G    DTL V SNLAA YDAMGR+E+AIEIL
Sbjct: 494 MGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIEDAIEIL 553

Query: 545 EDILKVREEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSNSLRIKKE-VA 578
           E +LK+REE LGTANP+ ++EK+RL ELLKEAGR+RN KAKSLQ L+  N+   KKE  A
Sbjct: 554 EQVLKLREEKLGTANPDFEDEKKRLAELLKEAGRSRNYKAKSLQNLIDPNARPPKKESSA 609

BLAST of Lsi09G016730 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 566.6 bits (1459), Expect = 2.3e-161
Identity = 309/532 (58.08%), Postives = 387/532 (72.74%), Query Frame = 0

Query: 5   TQTPHLHIQIPPIENDDTQHQNNNYKTMTPPWLKPIPSPSQSPQKLGPNQTW-------- 64
           + TP L + +P     DTQ  +N  +T     +K  PS + S  K  PN++         
Sbjct: 14  SDTPPLRVAVP-----DTQPLSNPPRTP----MKKTPSSTPSRSKPSPNRSTGKKDSPTV 73

Query: 65  --------------LDNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERF----- 124
                         LDNPD GP+LLKLARD I SGE P KALDYA+RA KSFER      
Sbjct: 74  SSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAIRATKSFERCCAAVA 133

Query: 125 -PSLG-------VELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYS 184
            P  G       ++L MSLHV+AAI+C LG+FDEA+P LE++I+V +P  GPDH+LA +S
Sbjct: 134 PPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVPDPTRGPDHSLAAFS 193

Query: 185 GYMQLGDTYSMLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKA 244
           G+MQLGDT SMLGQ+DRSI+CYE+GL IQI    D+DP+V ETCRYLAEA VQAMQF KA
Sbjct: 194 GHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVGETCRYLAEAYVQAMQFNKA 253

Query: 245 KKYCKKTLEIHKQHSPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDT 304
           ++ CKKTLEIH+ HS PAS +EA DRRLMA+ICEA GD E+ALEHLVLASMAM+A G ++
Sbjct: 254 EELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYENALEHLVLASMAMIASGQES 313

Query: 305 EVAAIDASIGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSK 364
           EVA+ID SIG+IY SLCRFDEA+F+YQKALT+F ++KG++H +VAS+F+RLAELY+RT K
Sbjct: 314 EVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAELYHRTGK 373

Query: 365 SREAKSYADNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILER 424
            RE+KSY +NALRIY KP  G + EEI+ GL EISA+YE+ +EPEEA  +LQ++ K+LE 
Sbjct: 374 LRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKSMKLLED 433

Query: 425 GGGGGRGSGSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGGAKSAMFGVLLNQ 484
             G        A+AG+EA+MGVM+Y VG+Y +AR AFE A+ KLR  G KSA FGV+LNQ
Sbjct: 434 KPG-----QQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRAAGEKSAFFGVVLNQ 493

Query: 485 MGLACIQLYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGR 502
           MGLAC+QL+KI EA +LF+EA+ +LE++ G    DTL V SNLAA YDAMGR
Sbjct: 494 MGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMGR 531

BLAST of Lsi09G016730 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 519.2 bits (1336), Expect = 4.2e-147
Identity = 282/523 (53.92%), Postives = 375/523 (71.70%), Query Frame = 0

Query: 44  SQSPQKLGPNQTWLDNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERF----PS 103
           S+    LG      ++P+ G  LLK AR+ + SGE+  KALD A+RA K FE+       
Sbjct: 135 SRKSPDLGKVSVDEESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQ 194

Query: 104 LGVELPMSLHVVAAIHCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYS 163
           LG+ L MSLH++AAI+  LG++++A+PVLE+SIE+    +G DHALAK++G MQLGD Y 
Sbjct: 195 LGLNLVMSLHILAAIYAGLGRYNDAVPVLERSIEIPMIEDGEDHALAKFAGCMQLGDMYG 254

Query: 164 MLGQLDRSISCYEDGLMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEI 223
           ++GQ++ SI  Y  GL IQ     +SD +V ETCRYLAEA+VQAMQFE+A + C+  L+I
Sbjct: 255 LMGQVENSIMLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDI 314

Query: 224 HKQH--SPPASPQEATDRRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDAS 283
           HK++  +  AS +EA DR+LM LIC+A GD E ALEH VLASMAM +  H  +VAA+D S
Sbjct: 315 HKENGAAATASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCS 374

Query: 284 IGDIYTSLCRFDEAIFAYQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYA 343
           IGD Y SL RFDEAIFAYQKAL +F   KG++H SVA +++RLA+LYN+  K+R++KSY 
Sbjct: 375 IGDAYMSLARFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYC 434

Query: 344 DNALRIYLKPPMGASCEEISSGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGS 403
           +NAL+IYLKP  G   EE+++G +EISA+Y++ NE ++A  +L+RA KI     G     
Sbjct: 435 ENALKIYLKPTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPG----- 494

Query: 404 GSRAVAGIEAQMGVMFYMVGKYGEARKAFEGAIGKLRGGG-AKSAMFGVLLNQMGLACIQ 463
               +AGIEAQMGV+ YM+G Y E+   F+ AI K R  G  K+A+FG+ LNQMGLAC+Q
Sbjct: 495 QQNTIAGIEAQMGVVTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQ 554

Query: 464 LYKIGEAIKLFQEAKLVLEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVR 523
            Y I EA  LF+EAK +LE++ G YH DTLAV SNLA  YDAMGR+++AIEILE ++  R
Sbjct: 555 RYAINEAADLFEEAKTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTR 614

Query: 524 EEMLGTANPEVDEEKRRLEELLKEAGRARNKKAKSLQILLGSN 560
           EE LGTANPEV++EK+RL  LLKEAGR R+K+ ++L  LL +N
Sbjct: 615 EEKLGTANPEVEDEKQRLAALLKEAGRGRSKRNRALLTLLDNN 652

BLAST of Lsi09G016730 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 506.9 bits (1304), Expect = 2.1e-143
Identity = 277/506 (54.74%), Postives = 365/506 (72.13%), Query Frame = 0

Query: 58  DNPDHGPYLLKLARDTIISGESPTKALDYAVRAAKSFERFPSLG---VELPMSLHVVAAI 117
           +N +   +LL  AR+ + SG+S  KAL+   RAAK FE     G   +E  M LHV AA+
Sbjct: 142 ENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHVTAAV 201

Query: 118 HCRLGQFDEAIPVLEQSIEVAEPGNGPDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDG 177
           HC+L +++EAIPVL++S+E+     G +HALAK++G MQLGDTY+M+GQL+ SISCY +G
Sbjct: 202 HCKLKEYNEAIPVLQRSVEIPVVEEGEEHALAKFAGLMQLGDTYAMVGQLESSISCYTEG 261

Query: 178 LMIQINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATD 237
           L IQ     ++DP+V ETCRYLAEA VQA++F++A++ C+  L IH++   P S  EA D
Sbjct: 262 LNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGLPGSIAEAAD 321

Query: 238 RRLMALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFA 297
           RRLM LICE  GD E+ALEHLVLASMAM A+G ++EVA +D SIGD Y SL RFDEAI A
Sbjct: 322 RRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSLSRFDEAICA 381

Query: 298 YQKALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCE 357
           YQK+LT   + KG++H +V S++IRLA+LYNRT K REAKSY +NALRIY    +  S E
Sbjct: 382 YQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYESHNLEISPE 441

Query: 358 EISSGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFY 417
           EI+SGL +IS + E+ NE E+A  +LQ+A KI     G         +AGIEAQMGV++Y
Sbjct: 442 EIASGLTDISVICESMNEVEQAITLLQKALKIYADSPG-----QKIMIAGIEAQMGVLYY 501

Query: 418 MVGKYGEARKAFEGAIGKLRGGGAK-SAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLV 477
           M+GKY E+   F+ AI KLR  G K S  FG+ LNQMGLACIQL  I EA++LF+EAK +
Sbjct: 502 MMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEEAKCI 561

Query: 478 LEEQYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRR 537
           LE++ G YH +TL + SNLA AYDA+GR+++AI++L  ++ VREE LGTANP  ++EKRR
Sbjct: 562 LEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTEDEKRR 621

Query: 538 LEELLKEAGRARNKKAKSLQILLGSN 560
           L +LLKEAG    +KAKSL+ L+ S+
Sbjct: 622 LAQLLKEAGNVTGRKAKSLKTLIDSD 642

BLAST of Lsi09G016730 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 133.3 bits (334), Expect = 6.4e-31
Identity = 133/493 (26.98%), Postives = 229/493 (46.45%), Query Frame = 0

Query: 67  LKLARDTIISGESPTKALDYAVRAAKSFE---RFPSLGVELPMSLHVVAAIHCRLGQFDE 126
           LKL       GE P K L YA +A KSF+     P+L V   M+  ++ + +  L +F +
Sbjct: 122 LKLGLHLDREGEDPEKVLSYADKALKSFDGDGNKPNLLV--AMASQLMGSANYGLKRFSD 181

Query: 127 AIPVLEQS--IEVAEPGNG----PDHALAKYSGYMQLGDTYSMLGQLDRSISCYEDGLMI 186
           ++  L ++  I V    +G     D     ++  ++L +  + +G+ + +I   +  L I
Sbjct: 182 SLGYLNRANRILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIENLKKSLEI 241

Query: 187 QINAFTDSDPQVAETCRYLAEANVQAMQFEKAKKYCKKTLEIHKQHSPPASPQEATDRRL 246
           +   F +   ++    R LA+A V  + F +A  Y  K LEIHK+     S + A DRRL
Sbjct: 242 KEMTFDEDSKEMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSAEVAQDRRL 301

Query: 247 MALICEALGDSESALEHLVLASMAMLAHGHDTEVAAIDASIGDIYTSLCRFDEAIFAYQK 306
           + +I   L   + ALE   L+   +   G   E+   +    ++  +L +++EAI   + 
Sbjct: 302 LGVIYSGLEQHDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYEEAIDILK- 361

Query: 307 ALTIFNSTKGDSHLSVASLFIRLAELYNRTSKSREAKSYADNALRIYLKPPMGASCEEIS 366
             ++   T  DS +  A +FI +++      K  E+K   + A  I L+    A   E++
Sbjct: 362 --SVVQQTDKDSEMR-AMVFISMSKALVNQQKFAESKRCLEFACEI-LEKKETALPVEVA 421

Query: 367 SGLVEISAVYEAANEPEEAFGVLQRARKILERGGGGGRGSGSRAVAGIEAQMGVMFYMVG 426
               E++  YE+ NE E A  +LQ+   ILE+        GS     + A++G +    G
Sbjct: 422 EAYSEVAMQYESMNEFETAISLLQKTLGILEKLPQEQHSEGS-----VSARIGWLLLFSG 481

Query: 427 KYGEARKAFEGAIGKLRGG-GAKSAMFGVLLNQMGLACIQLYKIGEAIKLFQEAKLVLEE 486
           +  +A    E A  +L+   GAK    G + N +G A ++L +   A ++F  AK +++ 
Sbjct: 482 RVSQAVPYLESAAERLKESFGAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDV 541

Query: 487 QYGVYHSDTLAVSSNLAAAYDAMGRVEEAIEILEDILKVREEMLGTANPEVDEEKRRLEE 546
             G  H D++    NL+ AY  MG    A+E  + ++   +    +A  E+ E KR LE+
Sbjct: 542 SLGPNHVDSIDACQNLSKAYAGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLED 601

Query: 547 L-LKEAGRARNKK 549
           L LK  G     K
Sbjct: 602 LRLKARGGVSTNK 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O816292.4e-18458.85Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 P... [more]
Q9LII85.8e-14653.92Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 P... [more]
F4HSX93.0e-14254.74Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 P... [more]
Q6AZT71.4e-0621.86Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
P0CI653.1e-0628.41Nephrocystin-3 OS=Danio rerio OX=7955 GN=nphp3 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
E5GCM79.7e-26989.62Kinesin light chain OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3CB069.7e-26989.62kinesin light chain 1-like OS=Cucumis melo OX=3656 GN=LOC103498798 PE=4 SV=1[more]
A0A6J1H7R76.5e-24179.68protein KINESIN LIGHT CHAIN-RELATED 1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0KCI92.9e-23390.99Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106760 PE=4 SV=1[more]
A0A6J1DAT91.1e-20380.67protein KINESIN LIGHT CHAIN-RELATED 1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_038906715.13.3e-29592.06protein KINESIN LIGHT CHAIN-RELATED 1-like [Benincasa hispida] >XP_038906716.1 p... [more]
XP_004140507.33.5e-28990.41protein KINESIN LIGHT CHAIN-RELATED 1 [Cucumis sativus] >KAE8646783.1 hypothetic... [more]
XP_008459760.12.0e-26889.62PREDICTED: kinesin light chain 1-like [Cucumis melo] >ADN34226.1 kinesin light c... [more]
KAG6575259.15.6e-24780.52Protein KINESIN LIGHT CHAIN-RELATED 1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022959334.11.3e-24079.68protein KINESIN LIGHT CHAIN-RELATED 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G10840.11.7e-18558.85Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.22.3e-16158.08Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.14.2e-14753.92Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G27500.12.1e-14354.74Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G31240.16.4e-3126.98Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 527..547
NoneNo IPR availablePFAMPF13424TPR_12coord: 446..518
e-value: 2.5E-10
score: 40.5
coord: 271..345
e-value: 2.3E-10
score: 40.6
coord: 108..177
e-value: 1.3E-9
score: 38.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR46284PROTEIN KINESIN LIGHT CHAIN-RELATED 3coord: 42..577
NoneNo IPR availablePANTHERPTHR46284:SF9KINESIN LIGHT CHAIN-LIKE PROTEINcoord: 42..577
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 190..223
e-value: 0.34
score: 20.0
coord: 105..138
e-value: 50.0
score: 8.8
coord: 272..305
e-value: 2.1
score: 17.4
coord: 444..477
e-value: 28.0
score: 11.0
coord: 314..347
e-value: 57.0
score: 8.3
coord: 486..519
e-value: 2.0
score: 17.4
coord: 148..181
e-value: 14.0
score: 13.8
coord: 403..436
e-value: 460.0
score: 0.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 101..258
e-value: 2.4E-21
score: 78.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 437..567
e-value: 6.1E-16
score: 60.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 259..434
e-value: 1.1E-34
score: 122.2
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 267..386
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 105..516

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G016730.1Lsi09G016730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0055028 cortical microtubule
molecular_function GO:0005515 protein binding