Lsi09G016560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G016560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cytochrome b5
Location	chr09: 24931374 .. 24933285 (+)
RNA-Seq Expression	Lsi09G016560
Synteny	Lsi09G016560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAAATACACCCACAAAGGGAACATTTTTTTGAAAGAGCAATCATTGAGAAGAAACAAGTAACTCTAGGCTCTCAATGTTTCCTTTTTTTCTTTTAATTTTTTTTTTTTTTATTTTTCTTCAAATTCTTCTATTTATGATCTGAAATATTAAATCTCTGTTCCAGGCAGGTGAAGAGAAAAGTTGATGGAAATGGATTCAAATTCTGAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCGGAAAGATTGCTGGCTTATAATCTCTGGATATGTATGTCCTTTTTTCCTTTATTTTTCTTCTGTTTACTCTCTCTTAGAATTGATTGGTTAATGGAGTTTTTCCAAAACAAATCAACTAAAATTTATTTTGGGTTTTATTTTTCTCACTTAATATCAATCCAATTAAAGTGTTTTAATGTACTACATATAAAAATCTCTAGATTAATGTTCATTTTTTCCTTCTTTCCTAGGATATTAAGCATCTATGATGTACATACAATATTTAAGAAAAAACAATCACTAATTTATAAAAGTGTATTTATTTGTTTTAGGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTCGGTGAGATGCTGCACTATTTCTAATCATACAACTCTCTAATTAATAGCTATTTCTAATGTTTTAAATTAGTAAAATAGATATGACATTTTTAACATGGTTTTAGTATACATTAACTCCTAGTTAAAATACCATACGTGTTTTGCATGTCATTTTAGTCTTTATTCTACTTTCTATATTTTTAATCAACTTTTAAAATTAGTCTTCATCGTTACTTTGAAGTTCATTTTTACCTCACCATTTAAAACAACAAAGTCTATAACTTTTTCTTGCGGTTGTGACTTCTCACCCATCACGAAATATACGAATCTCTAACGCGATTTTTTTAACAAAAAGAAAACTTCCCATTGACCGTTAGTGATCGCGGGCGCCCAACTACCGCGACATTCTAGTGGAGCCTGGTCACGATTCCTCAACAGTAAAAATTACAAAAAAAAAAAAATAGGCTCTTGGGATTCTATTTAATTAGTTTAAACGGGTTGTGATGGCAATTTAAAATCACAAACAGACTCCGCTAGAATTTTGCATTAGCATATTTAGGATTGAGTCACGATGCAAATACAAATCACGTTAGGCATGATCCGCATATTCCGTGATGGGTGAGAAGTCGCGACCTCGAGGAGAAATTTTAGACTCTTTAGTGCTCTGTGACGAAAGCAGAAGGTAAGTTTTCATTGGTTTATAGGATTAGGTCATTTCACATTAGCACATTTAGGATTGAGTCATCCATACAAAAACTTCCAGAACAATTATAATTTCTATTTGTTGTTAAAGAAAAAAAGCATAACATCAATATGTTAGAAGGAAAGTTAGCAGAGTAATTATTTCTAACAAAAAAAATATATATTTAAATTAAACAATTGAATCAGTGTAGAATTTATTGAAAGTATAAAGACTCAATTTAATTTGGGCATTTAAAAATAAAACAATAATGGAACTTCTAGTTTTGAGATTGATAGTACATGTATCTATATCAATTAAACTATATTCATTTTGACAACTGAAGTAGAAGTATGTTGAGGATTGAAGTGACAGATTTAAATGTGCAGGAAGAGATGCAACATTTGATTTTAAGAGTGTTGGACATAGCGAGTCAGCTCAAGAGAAGATGAAAAAGTATCGAATAGGAAAGATCGACATGTCAACTCTCCCTAGGAAACAGGCATATGTGGAAACTACACCATATCAGCCAACTCCAAGTCTATTTTCCTTCTCACCCATCCATGTTATAATCCCTATTTTGCTCTTGGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAAGCA

mRNA sequence

AAATAAATACACCCACAAAGGGAACATTTTTTTGAAAGAGCAATCATTGAGAAGAAACAAGCAGGTGAAGAGAAAAGTTGATGGAAATGGATTCAAATTCTGAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCGGAAAGATTGCTGGCTTATAATCTCTGGATATGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAAGTGTTGGACATAGCGAGTCAGCTCAAGAGAAGATGAAAAAGTATCGAATAGGAAAGATCGACATGTCAACTCTCCCTAGGAAACAGGCATATGTGGAAACTACACCATATCAGCCAACTCCAAGTCTATTTTCCTTCTCACCCATCCATGTTATAATCCCTATTTTGCTCTTGGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAAGCA

Coding sequence (CDS)

ATGGAAATGGATTCAAATTCTGAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCGGAAAGATTGCTGGCTTATAATCTCTGGATATGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAAGTGTTGGACATAGCGAGTCAGCTCAAGAGAAGATGAAAAAGTATCGAATAGGAAAGATCGACATGTCAACTCTCCCTAGGAAACAGGCATATGTGGAAACTACACCATATCAGCCAACTCCAAGTCTATTTTCCTTCTCACCCATCCATGTTATAATCCCTATTTTGCTCTTGGCCTTGGCTTTTGCATTTCCCTACTTCAAACTTAAAGCA

Protein sequence

MEMDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLASVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLALAFAFPYFKLKA

Homology

[Close]

BLAST of Lsi09G016560 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 1.0e-23
Identity = 63/136 (46.32%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M S+ +V SF+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL ++         
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP------TPSLFSFSPIHVIIP 122
            VGHS++A++ M KY IG+ID S++P  + YV   P QP      TP  F    +  ++P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

[Close]

BLAST of Lsi09G016560 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 3.9e-23
Identity = 62/136 (45.59%), Postives = 83/136 (61.03%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M S  +V  F+EV++HN  KDCWLIISG VYDVT F+ DHPGGDE+LL ++         
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP------TPSLFSFSPIHVIIP 122
            VGHS++A++ M+KY IG+ID ST+P  + YV   P QP      TP  F    +  ++P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

[Close]

BLAST of Lsi09G016560 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.1e-21
Identity = 55/132 (41.67%), Postives = 80/132 (60.61%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M   ++V++  EV++HN+ KDCWL+ISG VYDVT FL DHPGGDE+LL A+         
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP-----TPSLFSFSPIHVIIPI 120
            VGHS SA+  + +Y +G ID +T+P K  Y  T P QP       S F    +  ++P+
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

[Close]

BLAST of Lsi09G016560 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.8e-20
Identity = 51/126 (40.48%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M   +++++  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL ++         
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP-----TPSLFSFSPIHVIIPI 114
            VGHSESA+E M++Y +G+ID +T+P+K  Y  T P QP       S F    +  ++P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

[Close]

BLAST of Lsi09G016560 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 4.0e-20
Identity = 55/128 (42.97%), Postives = 77/128 (60.16%), Query Frame = 0


Query: 4   DSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS---------- 63
           + N +VY+ +EVAKHN + DCWLII G VY+V+ FL DHPGGD++LL ++          
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 64  VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP------TPSLFSFSPIHVIIPI 116
           VGHS +A+  M +Y +G ID ST+P +  YV   P QP      TP  F    +  ++P+
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYV--PPKQPHYNQDKTPE-FIIKILQFLVPL 122

[Close]

BLAST of Lsi09G016560 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 2.3e-55
Identity = 112/131 (85.50%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS+VYSFDEVAKHNH+KDCWLIISGYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSE AQEKMK YRIGKIDMSTLP+KQ YVE+TPYQPTPSLFSFSPIHV++PILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Lsi09G016560 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.7e-51
Identity = 106/131 (80.92%), Postives = 113/131 (86.26%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS+VYSFDEVAKHN +KDCWLIISGYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSE A EKMK Y+IGKIDMSTLP KQ YVE+ PYQ TPSLFSFSPIH+++PILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Lsi09G016560 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.3e-47
Identity = 96/131 (73.28%), Postives = 112/131 (85.50%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS+VY+FDEVAKHNH KDCWLIISG+VYDVTS+LPDHPGG ELL+LA          
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSESA+E+MKKY+IGKID  TLP +Q +VETTP++PTPSL SFSP+HV+IP+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Lsi09G016560 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.1e-47
Identity = 95/131 (72.52%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS++Y+F+EVAKHNH KDCWLIISGYVYDVTS+LPDHPGG ELL+L           
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSE+A+E MKKY+IGKID  TLP +Q YVETTPY+PTPSL SFSP+HV+IP+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Lsi09G016560 vs. ExPASy TrEMBL
Match: M5X125 (Cytochrome b5 heme-binding domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G194100 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.9e-28
Identity = 69/133 (51.88%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           M S+S+VY FDEVAKHNH+KDCW+IISG VYDVT FL DHPGGDE+LLLA          
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAY---VETTPYQPTPSLFSFSPIHVIIPILL 122
            VGHS+SA+E+M+K+ +GK+D ST+P + +Y    +TTP     S F    +  ++P+L+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTTPPSGQSSGFVVKLLQFLLPLLI 120

[Close]

BLAST of Lsi09G016560 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 224.6 bits (571), Expect = 4.8e-55
Identity = 112/131 (85.50%), Postives = 118/131 (90.08%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS+VYSFDEVAKHNH+KDCWLIISGYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSE AQEKMK YRIGKIDMSTLP+KQ YVE+TPYQPTPSLFSFSPIHV++PILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Lsi09G016560 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 223.4 bits (568), Expect = 1.1e-54
Identity = 110/133 (82.71%), Postives = 120/133 (90.23%), Query Frame = 0


Query: 1   MEMDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA-------- 60
           M+MDS+S++YSFDEV+KHNH +DCWLIISGYVYDVT+FLPDHPGG+ELLLLA        
Sbjct: 1   MQMDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFD 60

Query: 61  --SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLL 120
             SVGHSE AQEKMKKYRIGKIDMSTLPRKQ YVETTPYQPTPSLFSFSPIH++IPILLL
Sbjct: 61  FKSVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLL 120

Query: 121 ALAFAFPYFKLKA 124
           ALAFAFPYFKLKA
Sbjct: 121 ALAFAFPYFKLKA 133

[Close]

BLAST of Lsi09G016560 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 211.1 bits (536), Expect = 5.5e-51
Identity = 106/131 (80.92%), Postives = 113/131 (86.26%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS+VYSFDEVAKHN +KDCWLIISGYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSE A EKMK Y+IGKIDMSTLP KQ YVE+ PYQ TPSLFSFSPIH+++PILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of Lsi09G016560 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 198.0 bits (502), Expect = 4.8e-47
Identity = 96/131 (73.28%), Postives = 112/131 (85.50%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS+VY+FDEVAKHNH KDCWLIISG+VYDVTS+LPDHPGG ELL+LA          
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSESA+E+MKKY+IGKID  TLP +Q +VETTP++PTPSL SFSP+HV+IP+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Lsi09G016560 vs. NCBI nr
Match: XP_023000205.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 197.6 bits (501), Expect = 6.3e-47
Identity = 95/131 (72.52%), Postives = 110/131 (83.97%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLA---------- 62
           MDSNS++Y+F+EVAKHNH KDCWLIISGYVYDVTS+LPDHPGG ELL+L           
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 63  SVGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQPTPSLFSFSPIHVIIPILLLAL 122
           SVGHSE+A+E MKKY+IGKID  TLP +Q YVETTPY+PTPSL SFSP+HV+IP+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 123 AFAFPYFKLKA 124
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of Lsi09G016560 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 110.9 bits (276), Expect = 7.2e-25
Identity = 63/136 (46.32%), Postives = 85/136 (62.50%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M S+ +V SF+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL ++         
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP------TPSLFSFSPIHVIIP 122
            VGHS++A++ M KY IG+ID S++P  + YV   P QP      TP  F    +  ++P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

[Close]

BLAST of Lsi09G016560 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 100.1 bits (248), Expect = 1.3e-21
Identity = 51/126 (40.48%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M   +++++  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL ++         
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETTPYQP-----TPSLFSFSPIHVIIPI 114
            VGHSESA+E M++Y +G+ID +T+P+K  Y  T P QP       S F    +  ++P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

[Close]

BLAST of Lsi09G016560 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 97.8 bits (242), Expect = 6.3e-21
Identity = 53/137 (38.69%), Postives = 80/137 (58.39%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M  + +V++  EV++H+  KDCW++I G VYDVT FL DHPGGDE++L ++         
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTLPRKQAYVETT-----PYQPTPSLFSFSPIHVIIPI 122
            VGHS +A+  + +Y +G ID +T+P K  +V  T       Q   S F    +  ++P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 123 LLLALAFAFPYF-KLKA 124
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of Lsi09G016560 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 94.7 bits (234), Expect = 5.4e-20
Identity = 50/131 (38.17%), Postives = 77/131 (58.78%), Query Frame = 0


Query: 7   SEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS----------VGH 66
           + + SF +VAKH  + DCW++I G VYD+++F+ +HPGGD +LL  +          V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 67  SESAQEKMKKYRIGKIDMSTLPRKQAYV-----ETTPYQPTPSLFSFSPIHVIIPILLLA 123
           S+ A+E MKKY IG +D ST+P  Q Y+     E+T  + T        +  +IP+L+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

[Close]

BLAST of Lsi09G016560 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 91.3 bits (225), Expect = 5.9e-19
Identity = 44/126 (34.92%), Postives = 76/126 (60.32%), Query Frame = 0


Query: 3   MDSNSEVYSFDEVAKHNHRKDCWLIISGYVYDVTSFLPDHPGGDELLLLAS--------- 62
           M + +++YS +E A HN + DCW++I G VYDV+S++ +HPGGD++LL  +         
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 63  -VGHSESAQEKMKKYRIGKIDMSTL---PRKQAYVETTPYQPTPSLFSFSPIHVIIPILL 116
             GHS+ A+E M+KY IG++D S+L   P  + Y +  P      LF  +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

The following BLAST results are available for this feature:

BLAST of Lsi09G016560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	1.0e-23	46.32	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	3.9e-23	45.59	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	2.1e-21	41.67	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O48845	1.8e-20	40.48	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49100	4.0e-20	42.97	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of Lsi09G016560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CAE5	2.3e-55	85.50	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1	[more]
A0A0A0KA20	2.7e-51	80.92	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1HJ10	2.3e-47	73.28	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1	[more]
A0A6J1KHQ2	3.1e-47	72.52	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1	[more]
M5X125	1.9e-28	51.88	Cytochrome b5 heme-binding domain-containing protein OS=Prunus persica OX=3760 G...	[more]

BLAST of Lsi09G016560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459741.1	4.8e-55	85.50	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_038875836.1	1.1e-54	82.71	cytochrome b5-like [Benincasa hispida]	[more]
XP_004140502.1	5.5e-51	80.92	cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc...	[more]
XP_022964486.1	4.8e-47	73.28	cytochrome b5-like [Cucurbita moschata]	[more]
XP_023000205.1	6.3e-47	72.52	cytochrome b5-like [Cucurbita maxima]	[more]

BLAST of Lsi09G016560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	7.2e-25	46.32	cytochrome B5 isoform E	[more]
AT2G32720.1	1.3e-21	40.48	cytochrome B5 isoform B	[more]
AT5G48810.1	6.3e-21	38.69	cytochrome B5 isoform D	[more]
AT2G46650.1	5.4e-20	38.17	cytochrome B5 isoform C	[more]
AT1G26340.1	5.9e-19	34.92	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 10..73 e-value: 4.4E-19 score: 79.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 13..51 e-value: 2.1E-13 score: 50.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 7..73 score: 19.654137
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..77 e-value: 4.8E-24 score: 86.1
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 6..78
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 3..121
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 3..121
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 38..45

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G016560.1	Lsi09G016560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding