Lsi09G016010 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G016010
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Delta(8)-fatty-acid desaturase
Location	chr09: 24153292 .. 24154635 (-)
RNA-Seq Expression	Lsi09G016010
Synteny	Lsi09G016010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTGGAGAAGAAGTACATTACGTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTGTGGATCTCTATTCAGGGTAAGGTCTATAATGTCTCAGAATGGGCTAAAGAACATCCTGGTGGTGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACCGATGCATTCATCGCTTATCATCCTGGCACCGCTTGGGCGTATTTAGACCGGTTGTTCACTGGATTTCATCTCAAGGATTTTCAGGTCTCGGAGATTTCTAAGGATTACAGACGGCTTGCTTCTGAATTTTCGAAACAAGGTTTGTTTGAGAAGAAGGGGCATGTTACTATGTATTCTTTGATTTCTGTTGCTGTGATGATGTTTCTTGTGGTTTATGGGGTTTTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATCCAAAGTGCTTATGTGGGTCATGATTCTGGGCATTCTAATGTAATGTCCAGCCGCGGATTTAACAAATTGGCTCAAATTTTGTCTGGGAATTGCTTGACGGGGATTAGTATAGCCTGGTGGAAGTGGACTCATAATGCTCACCATATCTCTTGCAATAGCCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCTGTTTCTACTAGATTCTTTCGGTCTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTCGTCAGTTACCAGCATTTCACATTTTACCCTGTTATGTGTGTGGCTAGAGTTAATCTCTTTGTGCAGACATTGTTGCTCTTGTTTTCCACTAGAAGAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTGGTTTCCTATCTCCCCAATTGGCCTGAAAGGTTCATGTTTGTTCTTGCAAGTTTCGCTGTATGTTCTCTTCAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGACTGGTTTGAGAAGCAAACAAGTGGGACTTTGGATATCTCTTGTTCTACTTCGATGGACTGGTTTTTTGGTGGATTGCAGTTTCAGCTAGAGCATCATTTGTTCCCAAGGATGCCCAGATGCCAATTGAGGAAAATCTCTCCAATTGTGGTGGAACTTTGCAAGAAGCACAATCTTCCTTACACTAGTTTGTCATTTTGGGATGCTAATGTCCGCACCATTAAAACTCTTAGAACAGCTGCTTTGCAGGCTAGAGATTTGACCAACCCTGTTCCTAAGAACTTGCTCTGGGAAGCTGTGAACACCCATGGATGA

mRNA sequence

ATGGAGGTGGAGAAGAAGTACATTACGTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTGTGGATCTCTATTCAGGGTAAGGTCTATAATGTCTCAGAATGGGCTAAAGAACATCCTGGTGGTGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACCGATGCATTCATCGCTTATCATCCTGGCACCGCTTGGGCGTATTTAGACCGGTTGTTCACTGGATTTCATCTCAAGGATTTTCAGGTCTCGGAGATTTCTAAGGATTACAGACGGCTTGCTTCTGAATTTTCGAAACAAGGTTTGTTTGAGAAGAAGGGGCATGTTACTATGTATTCTTTGATTTCTGTTGCTGTGATGATGTTTCTTGTGGTTTATGGGGTTTTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATCCAAAGTGCTTATGTGGGTCATGATTCTGGGCATTCTAATGTAATGTCCAGCCGCGGATTTAACAAATTGGCTCAAATTTTGTCTGGGAATTGCTTGACGGGGATTAGTATAGCCTGGTGGAAGTGGACTCATAATGCTCACCATATCTCTTGCAATAGCCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCTGTTTCTACTAGATTCTTTCGGTCTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTCGTCAGTTACCAGCATTTCACATTTTACCCTGTTATGTGTGTGGCTAGAGTTAATCTCTTTGTGCAGACATTGTTGCTCTTGTTTTCCACTAGAAGAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTGGTTTCCTATCTCCCCAATTGGCCTGAAAGGTTCATGTTTGTTCTTGCAAGTTTCGCTGTATGTTCTCTTCAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGACTGGTTTGAGAAGCAAACAAGTGGGACTTTGGATATCTCTTGTTCTACTTCGATGGACTGGTTTTTTGGTGGATTGCAGTTTCAGCTAGAGCATCATTTGTTCCCAAGGATGCCCAGATGCCAATTGAGGAAAATCTCTCCAATTGTGGTGGAACTTTGCAAGAAGCACAATCTTCCTTACACTAGTTTGTCATTTTGGGATGCTAATGTCCGCACCATTAAAACTCTTAGAACAGCTGCTTTGCAGGCTAGAGATTTGACCAACCCTGTTCCTAAGAACTTGCTCTGGGAAGCTGTGAACACCCATGGATGA

Coding sequence (CDS)

ATGGAGGTGGAGAAGAAGTACATTACGTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTGTGGATCTCTATTCAGGGTAAGGTCTATAATGTCTCAGAATGGGCTAAAGAACATCCTGGTGGTGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACCGATGCATTCATCGCTTATCATCCTGGCACCGCTTGGGCGTATTTAGACCGGTTGTTCACTGGATTTCATCTCAAGGATTTTCAGGTCTCGGAGATTTCTAAGGATTACAGACGGCTTGCTTCTGAATTTTCGAAACAAGGTTTGTTTGAGAAGAAGGGGCATGTTACTATGTATTCTTTGATTTCTGTTGCTGTGATGATGTTTCTTGTGGTTTATGGGGTTTTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATCCAAAGTGCTTATGTGGGTCATGATTCTGGGCATTCTAATGTAATGTCCAGCCGCGGATTTAACAAATTGGCTCAAATTTTGTCTGGGAATTGCTTGACGGGGATTAGTATAGCCTGGTGGAAGTGGACTCATAATGCTCACCATATCTCTTGCAATAGCCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCTGTTTCTACTAGATTCTTTCGGTCTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTCGTCAGTTACCAGCATTTCACATTTTACCCTGTTATGTGTGTGGCTAGAGTTAATCTCTTTGTGCAGACATTGTTGCTCTTGTTTTCCACTAGAAGAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTGGTTTCCTATCTCCCCAATTGGCCTGAAAGGTTCATGTTTGTTCTTGCAAGTTTCGCTGTATGTTCTCTTCAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGACTGGTTTGAGAAGCAAACAAGTGGGACTTTGGATATCTCTTGTTCTACTTCGATGGACTGGTTTTTTGGTGGATTGCAGTTTCAGCTAGAGCATCATTTGTTCCCAAGGATGCCCAGATGCCAATTGAGGAAAATCTCTCCAATTGTGGTGGAACTTTGCAAGAAGCACAATCTTCCTTACACTAGTTTGTCATTTTGGGATGCTAATGTCCGCACCATTAAAACTCTTAGAACAGCTGCTTTGCAGGCTAGAGATTTGACCAACCCTGTTCCTAAGAACTTGCTCTGGGAAGCTGTGAACACCCATGGATGA

Protein sequence

MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIAYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAVMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSLARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSYLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTSMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLRTAALQARDLTNPVPKNLLWEAVNTHG

Homology

[Close]

BLAST of Lsi09G016010 vs. ExPASy Swiss-Prot
Match: Q3EBF7 (Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 1.2e-210
Identity = 332/446 (74.44%), Postives = 388/446 (87.00%), Query Frame = 0


Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           + +K+Y+TSE+L KHNKPGDLWISIQGKVY+VS+W K HPGG+   LNLAG DVTDAFIA
Sbjct: 4   QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAV 121
           YHPGTAW +L++L  G+H++D  VS++S+DYRRLA+EFSK+GLF+KKGHVT+Y+L  V V
Sbjct: 64  YHPGTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGV 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M+  V+YGVL   S+  HL  A++LG+LWIQSAYVGHDSGH  V S++  NKL Q+LSGN
Sbjct: 124 MLAAVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVST+FF S+TS FYGRKL FD L
Sbjct: 184 CLTGISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPL 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSY 301
           ARF +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVFWTWFPLLVS+
Sbjct: 244 ARFLISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSF 303

Query: 302 LPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTS 361
           LPNW ERF+FV  SFAV ++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDISC + 
Sbjct: 304 LPNWQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSF 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPY SLS+W+ANV TI+TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
            AA+QARD TNPV KNLLWEAVNTHG
Sbjct: 424 NAAIQARDATNPVLKNLLWEAVNTHG 449

[Close]

BLAST of Lsi09G016010 vs. ExPASy Swiss-Prot
Match: Q9ZRP7 (Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 4.6e-207
Identity = 330/446 (73.99%), Postives = 381/446 (85.43%), Query Frame = 0


Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           E EKKYIT+E+L KHNK GDLWI+IQGKVYNVS+W K HPGGDT  LNL G DVTDAFIA
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAV 121
           +HPGTAW +LD LFTG+H++DFQVSE+S+DYRR+A+EF K GLFE KGHVT+Y+L  VA 
Sbjct: 64  FHPGTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAA 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M   V+YGVL   SV  H   A +LG+LWIQSAY+GHDSGH  +MS++ +N+ AQ+LSGN
Sbjct: 124 MFLGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVST+FF SLTS FY RKL FD +
Sbjct: 184 CLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPV 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSY 301
           ARF VSYQHFT+YPVMC  R+NLF+QT LLLFS R VPDRALN  GILVFWTWFPLLVS 
Sbjct: 244 ARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSC 303

Query: 302 LPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTS 361
           LPNWPERF FV  SF V +LQHIQF LNHF+A+VYVG PTG+DWFEKQ +GT+DISC + 
Sbjct: 304 LPNWPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSY 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPY S+S+++ANV TI TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
           TAA QARD+ NPV KNL+WEA+NTHG
Sbjct: 424 TAAYQARDVANPVVKNLVWEALNTHG 449

[Close]

BLAST of Lsi09G016010 vs. ExPASy Swiss-Prot
Match: Q43469 (Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 3.8e-201
Identity = 319/443 (72.01%), Postives = 378/443 (85.33%), Query Frame = 0


Query: 5   KKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIAYHP 64
           KKYITS+EL KHN P DLWISI GKVYNV+EWAKEHPGGD P +NLAG DVTDAFIA+HP
Sbjct: 16  KKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAPLINLAGQDVTDAFIAFHP 75

Query: 65  GTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAVMMF 124
           GTAW +LD+LFTG+HLKD+QVS+IS+DYR+LASEF+K G+FEKKGH  +YSL  V++++ 
Sbjct: 76  GTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 135

Query: 125 LVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 184
             VYGVL S S  +H+    ILG+ W+Q AY+GHD+GH  +M++RG+NK A I  GNC+T
Sbjct: 136 ACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 195

Query: 185 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSLARF 244
           GISIAWWKWTHNAHHI+CNSLD+DPDLQH+P+ AVS++ F S+TS FYGR+L FD LARF
Sbjct: 196 GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSITSVFYGRQLTFDPLARF 255

Query: 245 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSYLPN 304
           FVSYQH+ +YP+MCVARVNL++QT+LLL S R++PDR LNI+G L+FWTWFPLLVS LPN
Sbjct: 256 FVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIFWTWFPLLVSRLPN 315

Query: 305 WPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTSMDW 364
           WPER  FVL SF V  +QHIQF LNHFS +VYVG P G++WFEKQT GT+DI+CS+ MDW
Sbjct: 316 WPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWFEKQTRGTIDIACSSWMDW 375

Query: 365 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLRTAA 424
           FFGGLQFQLEHHLFPR+PRC LR ISPI  ELCKK+NLPY SLSF+DANV T+KTLRTAA
Sbjct: 376 FFGGLQFQLEHHLFPRLPRCHLRSISPICRELCKKYNLPYVSLSFYDANVTTLKTLRTAA 435

Query: 425 LQARDLTNPVPKNLLWEAVNTHG 448
           LQARDLTNP P+NL WEA NTHG
Sbjct: 436 LQARDLTNPAPQNLAWEAFNTHG 458

[Close]

BLAST of Lsi09G016010 vs. ExPASy Swiss-Prot
Match: Q9FR82 (Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 6.0e-199
Identity = 328/447 (73.38%), Postives = 380/447 (85.01%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME  KKYI+  EL KHN+ GD+WISIQGKVYNV++W K+HPGGD P +NLAG D TDAFI
Sbjct: 1   MEGTKKYISVGELEKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAW  L+ LFTG+HL+D+ VSEISKDYR+LASEFSK GLFEKKGH  +Y L  +A
Sbjct: 61  AYHPGTAWKNLENLFTGYHLEDYLVSEISKDYRKLASEFSKAGLFEKKGHTVIYCLSFIA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +++   VYGVL S S+ VH+    +LGM +IQ+AY+GHDSGH  +MSS+G+NK AQ+L+G
Sbjct: 121 LLLCGCVYGVLCSNSLWVHMLSGAMLGMCFIQAAYLGHDSGHYTMMSSKGYNKFAQVLNG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHI+CNSLD+DPDLQH+PVFAV + FF+SLTS FYGR+L FD 
Sbjct: 181 NCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPVFAVPSSFFKSLTSRFYGRELTFDG 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           L+RF VSYQHFT Y VM   R+NL+VQT LLLFSTR+VPDRALNI+GILV+WTWFP LVS
Sbjct: 241 LSRFLVSYQHFTIYLVMIFGRINLYVQTFLLLFSTRKVPDRALNIIGILVYWTWFPYLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
            LPNW ER +FVL  F+V +LQHIQF LNHF+A+VYVG PTG +WFEKQ +GT+DISCS+
Sbjct: 301 CLPNWNERVLFVLTCFSVTALQHIQFTLNHFAADVYVGPPTGTNWFEKQAAGTIDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLR ISPIV + CKKHNLPY SLSF+DANV TIKTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRNISPIVQDYCKKHNLPYRSLSFFDANVATIKTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLT  VP+NLLWEA NTHG
Sbjct: 421 RTAALQARDLT-VVPQNLLWEAFNTHG 446

[Close]

BLAST of Lsi09G016010 vs. ExPASy Swiss-Prot
Match: O04353 (Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 4.5e-178
Identity = 282/443 (63.66%), Postives = 352/443 (79.46%), Query Frame = 0


Query: 5   KKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIAYHP 64
           KKYITS+EL  H+KPGDLWISIQGK Y+VS+W K+HPGG  P  +LAG +VTDAF+A+HP
Sbjct: 6   KKYITSDELKNHDKPGDLWISIQGKAYDVSDWVKDHPGGSFPLKSLAGQEVTDAFVAFHP 65

Query: 65  GTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAVMMF 124
            + W  LD+ FTG++LKD+ VSE+SKDYR+L  EFSK GL++KKGH+   +L  +A++  
Sbjct: 66  ASTWKNLDKFFTGYYLKDYSVSEVSKDYRKLVFEFSKMGLYDKKGHIMFATLCFIAMLFA 125

Query: 125 LVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 184
           + VYGVL  E VLVHL    ++G LWIQS ++GHD+GH  V+S    NK   I + NCL+
Sbjct: 126 MSVYGVLFCEGVLVHLFSGCLMGFLWIQSGWIGHDAGHYMVVSDSRLNKFMGIFAANCLS 185

Query: 185 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSLARF 244
           GISI WWKW HNAHHI+CNSL++DPDLQ+IP   VS++FF SLTSHFY ++L FDSL+RF
Sbjct: 186 GISIGWWKWNHNAHHIACNSLEYDPDLQYIPFLVVSSKFFGSLTSHFYEKRLTFDSLSRF 245

Query: 245 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSYLPN 304
           FVSYQH+TFYP+MC AR+N++VQ+L++L + R V  RA  ++G LVF  W+PLLVS LPN
Sbjct: 246 FVSYQHWTFYPIMCAARLNMYVQSLIMLLTKRNVSYRAHELLGCLVFSIWYPLLVSCLPN 305

Query: 305 WPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTSMDW 364
           W ER MFV+AS +V  +Q +QF LNHFS++VYVGKP GN+WFEKQT GTLDISC   MDW
Sbjct: 306 WGERIMFVIASLSVTGMQQVQFSLNHFSSSVYVGKPKGNNWFEKQTDGTLDISCPPWMDW 365

Query: 365 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLRTAA 424
           F GGLQFQ+EHHLFP+MPRC LRKISP V+ELCKKHNLPY   SF  AN  T++TLR  A
Sbjct: 366 FHGGLQFQIEHHLFPKMPRCNLRKISPYVIELCKKHNLPYNYASFSKANEMTLRTLRNTA 425

Query: 425 LQARDLTNPVPKNLLWEAVNTHG 448
           LQARD+T P+PKNL+WEA++THG
Sbjct: 426 LQARDITKPLPKNLVWEALHTHG 448

[Close]

BLAST of Lsi09G016010 vs. ExPASy TrEMBL
Match: A0A0A0KA29 (Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.2e-261
Identity = 435/447 (97.32%), Postives = 442/447 (98.88%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNV+EWAKEHPGGDTPF+NLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYR+LASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVST FFR+LTSHFYGRKL+FDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQ SGTLDISCST
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. ExPASy TrEMBL
Match: A0A5D3CS55 (Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00250 PE=4 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.2e-260
Identity = 434/447 (97.09%), Postives = 440/447 (98.43%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNV+EWAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS  FFR+LTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. ExPASy TrEMBL
Match: A0A1S3C7C0 (delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.2e-260
Identity = 434/447 (97.09%), Postives = 440/447 (98.43%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNV+EWAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS  FFR+LTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. ExPASy TrEMBL
Match: A0A6J1KW79 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499262 PE=4 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.3e-254
Identity = 422/447 (94.41%), Postives = 434/447 (97.09%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNV++WAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVST FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. ExPASy TrEMBL
Match: A0A6J1H485 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460269 PE=4 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.2e-254
Identity = 421/447 (94.18%), Postives = 434/447 (97.09%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNV++WAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFS+QGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVST FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTQGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. NCBI nr
Match: XP_031743128.1 (delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolipid desaturase [Cucumis sativus] >KGN45699.1 hypothetical protein Csa_005537 [Cucumis sativus])

HSP 1 Score: 911.8 bits (2355), Expect = 2.4e-261
Identity = 435/447 (97.32%), Postives = 442/447 (98.88%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNV+EWAKEHPGGDTPF+NLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYR+LASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVST FFR+LTSHFYGRKL+FDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQ SGTLDISCST
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. NCBI nr
Match: XP_008458161.1 (PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-fatty-acid desaturase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 907.5 bits (2344), Expect = 4.5e-260
Identity = 434/447 (97.09%), Postives = 440/447 (98.43%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNV+EWAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS  FFR+LTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. NCBI nr
Match: XP_038874611.1 (delta(8)-fatty-acid desaturase 2 [Benincasa hispida])

HSP 1 Score: 905.2 bits (2338), Expect = 2.2e-259
Identity = 433/447 (96.87%), Postives = 439/447 (98.21%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNV+EWAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGF+LKDFQVSE+SKDYRRLASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFYLKDFQVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHL CA+ILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLVCALILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFRSLTSHFYGRKLKFD 
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSAKFFRSLTSHFYGRKLKFDG 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGI VFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGIFVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNW ER MFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST
Sbjct: 301 YLPNWSERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTIKTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIKTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. NCBI nr
Match: XP_023006577.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima])

HSP 1 Score: 887.5 bits (2292), Expect = 4.8e-254
Identity = 422/447 (94.41%), Postives = 434/447 (97.09%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNV++WAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFSKQGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVST FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. NCBI nr
Match: XP_022959210.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >XP_022959211.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >KAG7013765.1 Delta(8)-fatty-acid desaturase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 886.3 bits (2289), Expect = 1.1e-253
Identity = 421/447 (94.18%), Postives = 434/447 (97.09%), Query Frame = 0


Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNV++WAKEHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYRRLASEFS+QGLFEKKGHVTMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVST FF+SLTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTR+VPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360
           YLPNWPER MFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTQGTLDISCSS 360

Query: 361 SMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTL 420
            MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPY SLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

[Close]

BLAST of Lsi09G016010 vs. TAIR 10
Match: AT2G46210.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 733.8 bits (1893), Expect = 8.3e-212
Identity = 332/446 (74.44%), Postives = 388/446 (87.00%), Query Frame = 0


Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           + +K+Y+TSE+L KHNKPGDLWISIQGKVY+VS+W K HPGG+   LNLAG DVTDAFIA
Sbjct: 4   QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAV 121
           YHPGTAW +L++L  G+H++D  VS++S+DYRRLA+EFSK+GLF+KKGHVT+Y+L  V V
Sbjct: 64  YHPGTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGV 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M+  V+YGVL   S+  HL  A++LG+LWIQSAYVGHDSGH  V S++  NKL Q+LSGN
Sbjct: 124 MLAAVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVST+FF S+TS FYGRKL FD L
Sbjct: 184 CLTGISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPL 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSY 301
           ARF +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVFWTWFPLLVS+
Sbjct: 244 ARFLISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSF 303

Query: 302 LPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTS 361
           LPNW ERF+FV  SFAV ++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDISC + 
Sbjct: 304 LPNWQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSF 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPY SLS+W+ANV TI+TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
            AA+QARD TNPV KNLLWEAVNTHG
Sbjct: 424 NAAIQARDATNPVLKNLLWEAVNTHG 449

[Close]

BLAST of Lsi09G016010 vs. TAIR 10
Match: AT3G61580.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 721.8 bits (1862), Expect = 3.3e-208
Identity = 330/446 (73.99%), Postives = 381/446 (85.43%), Query Frame = 0


Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAFIA 61
           E EKKYIT+E+L KHNK GDLWI+IQGKVYNVS+W K HPGGDT  LNL G DVTDAFIA
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRRLASEFSKQGLFEKKGHVTMYSLISVAV 121
           +HPGTAW +LD LFTG+H++DFQVSE+S+DYRR+A+EF K GLFE KGHVT+Y+L  VA 
Sbjct: 64  FHPGTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAA 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M   V+YGVL   SV  H   A +LG+LWIQSAY+GHDSGH  +MS++ +N+ AQ+LSGN
Sbjct: 124 MFLGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTRFFRSLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVST+FF SLTS FY RKL FD +
Sbjct: 184 CLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPV 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRRVPDRALNIMGILVFWTWFPLLVSY 301
           ARF VSYQHFT+YPVMC  R+NLF+QT LLLFS R VPDRALN  GILVFWTWFPLLVS 
Sbjct: 244 ARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSC 303

Query: 302 LPNWPERFMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSTS 361
           LPNWPERF FV  SF V +LQHIQF LNHF+A+VYVG PTG+DWFEKQ +GT+DISC + 
Sbjct: 304 LPNWPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSY 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYTSLSFWDANVRTIKTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPY S+S+++ANV TI TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
           TAA QARD+ NPV KNL+WEA+NTHG
Sbjct: 424 TAAYQARDVANPVVKNLVWEALNTHG 449

[Close]

BLAST of Lsi09G016010 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-12
Identity = 47/121 (38.84%), Postives = 66/121 (54.55%), Query Frame = 0


Query: 6   KYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAF-IAYHP 65
           K  + EE   HNK  D W+ I GKVY+VS +  EHPGGD   L +AG D TD F  A H 
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  GTAWAYLDRLFTGFHLKDFQVSEIS--KDYRRLASEFSKQGLFE--KKGHVTMYSLISVA 122
             A   +++ F G  L +  + EI   K Y++   + S Q LF+  K+  V   S+I+++
Sbjct: 66  KDARELMEKYFIG-ELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSIITIS 125

[Close]

BLAST of Lsi09G016010 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 67.8 bits (164), Expect = 2.6e-11
Identity = 32/78 (41.03%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 1  MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAF- 60
          M  ++K ++ EE+ KHNK  D W+ I GKVY+V+ +  +HPGGD   L+  G D T+ F 
Sbjct: 1  MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61 IAYHPGTAWAYLDRLFTG 78
             H  TA   +D+ F G
Sbjct: 61 DVGHSDTARDMMDKYFIG 78

[Close]

BLAST of Lsi09G016010 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 27/56 (48.21%), Postives = 36/56 (64.29%), Query Frame = 0


Query: 4  EKKYITSEELLKHNKPGDLWISIQGKVYNVSEWAKEHPGGDTPFLNLAGHDVTDAF 60
          E K  T  E+ +HN+  D WI I GKVYNV+++ ++HPGGD   L+  G D TD F
Sbjct: 4  EAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDF 59

The following BLAST results are available for this feature:

BLAST of Lsi09G016010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3EBF7	1.2e-210	74.44	Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV...	[more]
Q9ZRP7	4.6e-207	73.99	Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV...	[more]
Q43469	3.8e-201	72.01	Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1	[more]
Q9FR82	6.0e-199	73.38	Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1	[more]
O04353	4.5e-178	63.66	Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1	[more]

BLAST of Lsi09G016010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KA29	1.2e-261	97.32	Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 S...	[more]
A0A5D3CS55	2.2e-260	97.09	Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C7C0	2.2e-260	97.09	delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV...	[more]
A0A6J1KW79	2.3e-254	94.41	delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149926...	[more]
A0A6J1H485	5.2e-254	94.18	delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460...	[more]

BLAST of Lsi09G016010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031743128.1	2.4e-261	97.32	delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolip...	[more]
XP_008458161.1	4.5e-260	97.09	PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-...	[more]
XP_038874611.1	2.2e-259	96.87	delta(8)-fatty-acid desaturase 2 [Benincasa hispida]	[more]
XP_023006577.1	4.8e-254	94.41	delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8...	[more]
XP_022959210.1	1.1e-253	94.18	delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >XP_022959211.1 delta...	[more]

BLAST of Lsi09G016010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46210.1	8.3e-212	74.44	Fatty acid/sphingolipid desaturase	[more]
AT3G61580.1	3.3e-208	73.99	Fatty acid/sphingolipid desaturase	[more]
AT1G26340.1	1.0e-12	38.84	cytochrome B5 isoform A	[more]
AT5G53560.1	2.6e-11	41.03	cytochrome B5 isoform E	[more]
AT2G32720.1	4.8e-10	48.21	cytochrome B5 isoform B	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..80 e-value: 1.1E-19 score: 81.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..77 e-value: 1.4E-21 score: 76.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..89 score: 22.376854
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..78 e-value: 6.8E-21 score: 75.9
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..86
IPR012171	Fatty acid desaturase	PIRSF	PIRSF015921	FA_sphingolip_des	coord: 1..434 e-value: 4.2E-127 score: 422.7
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 137..406 e-value: 3.0E-34 score: 119.0
None	No IPR available	PANTHER	PTHR19353:SF28	DELTA(8)-FATTY-ACID DESATURASE 2	coord: 1..446
None	No IPR available	PANTHER	PTHR19353	FATTY ACID DESATURASE 2	coord: 1..446
None	No IPR available	CDD	cd03506	Delta6-FADS-like	coord: 140..402 e-value: 5.10216E-62 score: 198.249

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G016010.1	Lsi09G016010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function	GO:0016491	oxidoreductase activity