Lsi09G015940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G015940
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	sphingoid long-chain bases kinase 2, mitochondrial
Location	chr09: 24051587 .. 24054905 (-)
RNA-Seq Expression	Lsi09G015940
Synteny	Lsi09G015940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTACTCATAAGATCAGTTCGAGAAAGAAAAAAAAAAAAAAAGAGATCTTTAATCTGACCATTGTTAAGCTCTAAAGTATGGTTTTGGAAAGCCAGAGATGAGAATAACCCACACAAAAATTAATACCAAACATTATCCAAATCTACAGTTTTTTTTTCCTTCAAAATAATCTGAATCAAACGGCACCCAGCAATGAAATTCTGATTTAGTGAGATCAAATGGACATATCGCTGCAAATCTTGAAGAAGCCCGTGAGATTTGAGATTTGAAGAAGCCCGTGAGATTTGAGATTTGAAGAAGCCGCAAATCTGCGTTAATGTCGATGTGAAGCTTTACTAATCATCTCCCTTAATGTTATTTCCCATCTTCTCTTCGCATTATTATTATTATTCTCTCCAAATCACACTCTATACTTCCCTAATCTATCGGACGCGGAAATTTCAGCGATTAAGAAAATGGTTGTTTCCTTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAAAACCCTTTATCACCAGGGCCGAGCAGCCCATCGCGCCTGATCTTTATGCCGATCGGTCAATTTTTCATCGTGGATCGTCTTCTTTCTCTTCTTCTTCTGGCCGTCGCGATCTTGTTTTCGTTGTTAATCCCCGCGGTTCGTTTCTTGTCTCTTAATTTTCAGTTTCGTTCGTAGGGAGAGGAATTTAGTTGAGAAATGGAAAATTTAGGATGTGGGTTTGTTGTATTTATGTCGTAATTGAAGATTGTAATGCCTTGAGTTTATTGGAACTTCACCTGAGATTATACTTTTCTGTGTGCTTGTCAAGTTCTTGTGCTATTGGCCATTGGGTGATTTCTAGTTTTGGATTTGTCTCATATTTTAGCTCCTATGCAGGTGCAAATGGCAAGACGGTGAAGGAATGGAAGAAGCTCGTTCCATATCTACGCACTCGCCTTGATGAACATTATAATGTGAGTTATTATTATTATTTATTTTTTCTCTTGGTATCTTTAGGGAATGCGTATGGGTCCAAATTCCAATGGTGTTTCTGGGTCTCAGCTTCACTATTTCCATTGAAGTTAATCTAGCTTATTGAGTTATGTTTAAGGAGCTTGTTGGAGCAAATGATTTGCAAAAGAAATACTAGATTTAAAGACTTTGGGAGGGAGCTATTTTCTGACTTATTTTTTCGTTTTATGACAACACACAAGTTCAGTTAAACATGTTTGGCATTTGGATATATGTTTTTTAGGACTTGTTTTCCATATTTTTCTTTCTATTTGTTGGCCTGTTTCCTAGAATAACTGAATTTGTTTGTTAAGCCTCCCATTCTGAATGTTTATGCTAGAAGGGACAAAAAAAGCAATTTTGTCACAAGTAGCTAGGATAAGTAGTATTTTTTATTACTAGGATTATTAGTAGTTATTATATTTGAATTTTGTGTGTTAGGAAGTTATAACTTCCTTTGTTGGTTATGATTGTGCCCCTCTCGAATTGGTCATACCTGATAGGTTGTATGTTCTCACTAGAGAATTTTTAAGTAAATAAAGGATACCTATCATTGTTCTCCCTTAGCCTTGACATTGATGAAAGGAGAGTATTGCCAAATTTTCAAAAGGATCACAAATATGTGCTTGCTTGGACTTAAGACTGAGAAATCTAGTTTTAGTATTGATTAAGTATTCCGTTCTTTACATACAACTCACATAAAGATTGTCCAACAACCAACCATTTTAATTGAATAACCATGTGATTCTAATTAAACCAGCCAGTTGACTATTGCTTTGATCTACATGGATATCATCTTCAATGACAAAGGAAAACCTTTTTGATAGACCCCTGGATAATATACCCTTTTAGGTTCTCTCTTTGTGTAAAATTTTTTCTTATTTTCATGGCTAGCTATTGTCTATTCTCTTTTGGCCAATTGGTCCTTTTTGTAGCCTCCAAAGGCTCAGGTTTGCTGCTTTCCTGTATGTTTTATCTCATCAATGAAATGCTAGAATATTTCTCATTTTAAAAAATTATAATAAATAGATAATAAAAAGACAGTACCTTCTATTATCATTGGCATATTTGTATCTTATTTTTACATTTTCCACTGATCTGACTTTTTGTTTCTTTAAATGAACTATTATTTGCTTTTATTGAGATTTCAAACTGGTTACTGATCAGTATCTTCATGCATTCTTTTTGTTATTTCAATTTATTTTTCCAAATTTCATTGAGGAGCCTGGAAAATCATAATAGCTCGGCTCCTTATCATGTTATAATGGAGTACATAAGCGAATGGTTCCATGTGCACTAATTTCTGTATGTTGAAATTTATCTTTCAACTAAACAATTCACTTCTGTGGACAGATATGTGAATCTTTGACGTCAGGCCCTTGCCATGCTATTGATATAACTAGGGAGGTTTGTCTGGATTCTTTCTTTGTGGGATTCAAATAGTTACTATCAACCCCTTTTTGTTGACTTGATGACTTATAGGCAATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTACGACTCATTTCTGTTAGTTCATTTTTCAATGCTTGGCTCAGGCTTGGTTTTCTAAATTACCTTTTCTTTTTGTTGGTTTTGGTTTCGGTTAAGCCAGAACCAAGCCAAACTGATTGCACACCTAAGTTGAATGATGTGATTGCCTGATTGGTGTATGACCAGTGTTTACTTTTGTTTCTCTTGGACTCCTCCAAACTTCTTGAAGAAGGCAGTGGTGTATGACCAGTGTTTATTTTTATCTATATATAGTTTTTGAACCCTGAGATTCCAGTGGTAACATCTAACCATTAGTGTTGCTTGAGTATGTTTTCTTCTCTTACTACTTAGCACTTGATCAAGGTCCGTATTCTTTAAAAATAACATTTTAACTTGGCTTTGATTCTAGCAACCCTCTTGTATTGTCCATTAGATGTCCATTAGATATAAAGCAAGCATCTTTCAAGGCTTTTAGTGAAAGTTTCTAGGATTGAAGATCAGTTCTATACATTTGTCTTGATGGACGAGGGGATTAACCTGCCTTCTGATTGACAGGTGGTTAATGGCTTCTTTTGGGCTGGTAAACCTATTGTTAATAAGGATGGAGAAGCTTGTAAATCAACAGCACTTGGTGTAAGAGATTCCTGCATAGTTACAATCTTAAGCTATCTATTTCCGTTATCTACCATAACTGGTAGTTATCAGTCTGTACTGACTCGTTCAGATTATTTTCATGAACTTATGCTATTGAATTGTCGGACGACATATATTATTGTGCCTACTTGCAGATTATACCCTTAG

mRNA sequence

TTTACTCATAAGATCAGTTCGAGAAAGAAAAAAAAAAAAAAAGAGATCTTTAATCTGACCATTGTTAAGCTCTAAAGTATGGTTTTGGAAAGCCAGAGATGAGAATAACCCACACAAAAATTAATACCAAACATTATCCAAATCTACAGTTTTTTTTTCCTTCAAAATAATCTGAATCAAACGGCACCCAGCAATGAAATTCTGATTTAGTGAGATCAAATGGACATATCGCTGCAAATCTTGAAGAAGCCCGTGAGATTTGAGATTTGAAGAAGCCCGTGAGATTTGAGATTTGAAGAAGCCGCAAATCTGCGTTAATGTCGATGTGAAGCTTTACTAATCATCTCCCTTAATCGATTAAGAAAATGGTTGTTTCCTTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAAAACCCTTTATCACCAGGGCCGAGCAGCCCATCGCGCCTGATCTTTATGCCGATCGGTCAATTTTTCATCGTGGATCGTCTTCTTTCTCTTCTTCTTCTGGCCGTCGCGATCTTGTTTTCGTTGTTAATCCCCGCGGTGCAAATGGCAAGACGGTGAAGGAATGGAAGAAGCTCGTTCCATATCTACGCACTCGCCTTGATGAACATTATAATATATGTGAATCTTTGACGTCAGGCCCTTGCCATGCTATTGATATAACTAGGGAGGCAATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTGGTTAATGGCTTCTTTTGGGCTGGTAAACCTATTGTTAATAAGGATGGAGAAGCTTGTAAATCAACAGCACTTGGTGTAAGAGATTCCTGCATAGTTACAATCTTAAGCTATCTATTTCCGTTATCTACCATAACTGGTAGTTATCAGTCTGTACTGACTCGTTCAGATTATTTTCATGAACTTATGCTATTGAATTGTCGGACGACATATATTATTGTGCCTACTTGCAGATTATACCCTTAG

Coding sequence (CDS)

ATGGTTGTTTCCTTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAAAACCCTTTATCACCAGGGCCGAGCAGCCCATCGCGCCTGATCTTTATGCCGATCGGTCAATTTTTCATCGTGGATCGTCTTCTTTCTCTTCTTCTTCTGGCCGTCGCGATCTTGTTTTCGTTGTTAATCCCCGCGGTGCAAATGGCAAGACGGTGAAGGAATGGAAGAAGCTCGTTCCATATCTACGCACTCGCCTTGATGAACATTATAATATATGTGAATCTTTGACGTCAGGCCCTTGCCATGCTATTGATATAACTAGGGAGGCAATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTGGTTAATGGCTTCTTTTGGGCTGGTAAACCTATTGTTAATAAGGATGGAGAAGCTTGTAAATCAACAGCACTTGGTGTAAGAGATTCCTGCATAGTTACAATCTTAAGCTATCTATTTCCGTTATCTACCATAACTGGTAGTTATCAGTCTGTACTGACTCGTTCAGATTATTTTCATGAACTTATGCTATTGAATTGTCGGACGACATATATTATTGTGCCTACTTGCAGATTATACCCTTAG

Protein sequence

MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNGFFWAGKPIVNKDGEACKSTALGVRDSCIVTILSYLFPLSTITGSYQSVLTRSDYFHELMLLNCRTTYIIVPTCRLYP

Homology

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BLAST of Lsi09G015940 vs. ExPASy Swiss-Prot
Match: O82359 (Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LCKB2 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.9e-43
Identity = 89/143 (62.24%), Postives = 106/143 (74.13%), Query Frame = 0


Query: 11  LGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVK 70
           L P  A +P   R +Q I  D +  R      +++  SSSG RDLVFVVNP+GANG+T K
Sbjct: 10  LCPFTAKQPSFLRKQQRITSDRFTFRGGGGGDAAAVVSSSGLRDLVFVVNPQGANGRTAK 69

Query: 71  EWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNG 130
           EWKKL+P+LR+RL +  N+ E LTSGP HAIDITREAIR+GADAVIAVGGDGT HEVVNG
Sbjct: 70  EWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVVNG 129

Query: 131 FFWAGKPIVNKDGEACKSTALGV 154
           FFW GKP+    GEA +STALG+
Sbjct: 130 FFWEGKPVGYLSGEASRSTALGL 152

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BLAST of Lsi09G015940 vs. ExPASy Swiss-Prot
Match: O14159 (Sphingoid long chain base kinase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=lcb4 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.4e-06
Identity = 39/130 (30.00%), Postives = 58/130 (44.62%), Query Frame = 0


Query: 12  GPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSS----------SSGRRDLVFVVNP 71
           GP+  T  +++R+ + +A   + D   F   S  F               RR +VF +NP
Sbjct: 60  GPNSVTLSYVSRSSK-VATKCWVD---FVENSDQFCEYLLDVAYKGIKRSRRFIVF-INP 119

Query: 72  RGANGKTVKEWKKLVPYLRTRLDEHYNICE-SLTSGPCHAIDITREAIREGADAVIAVGG 131
            G  GK    W+     +       ++ICE  LT    HA  I +       D +++VGG
Sbjct: 120 HGGKGKAKHIWESEAEPV---FSSAHSICEVVLTRRKDHAKSIAKNLDVGSYDGILSVGG 179

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BLAST of Lsi09G015940 vs. ExPASy Swiss-Prot
Match: Q9LRB0 (Sphingoid long-chain bases kinase 1 OS=Arabidopsis thaliana OX=3702 GN=LCBK1 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.0e-04
Identity = 33/133 (24.81%), Postives = 62/133 (46.62%), Query Frame = 0


Query: 55  LVFVVNPRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADA 114
           ++ ++NPR  +G+++K +  +V  +         + ++  +G    +  T + I   +D 
Sbjct: 250 MLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVD-INLCSDG 309

Query: 115 VIAVGGDGTFHEVVNGFFWAGKPIVNKDGEACK--STALGVRDSCIVTILSYLFPLSTIT 174
           +I VGGDG  +EV+NG      P   K+G +        G  +S + T+L    P+S   
Sbjct: 310 IICVGGDGIINEVLNGLLTRSNP---KEGVSIPIGIVPAGSDNSLVWTVLGVRDPISAAL 369

Query: 175 GSYQSVLTRSDYF 186
              +  LT +D F
Sbjct: 370 SIVKGGLTATDVF 378

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BLAST of Lsi09G015940 vs. ExPASy Swiss-Prot
Match: O34799 (Putative lipid kinase YtlR OS=Bacillus subtilis (strain 168) OX=224308 GN=ytlR PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-04
Identity = 29/77 (37.66%), Postives = 41/77 (53.25%), Query Frame = 0


Query: 57  FVVNPRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITRE--AIRE-GAD 116
           F++NP   +   ++ WK +   L  R  EH +    LT  P HA  + R+   I+E    
Sbjct: 6   FIINPTAGHRNGLRVWKSIQKELIKRKVEHRSF---LTEHPGHAEVLARQISTIQEYKLK 65

Query: 117 AVIAVGGDGTFHEVVNG 131
            +I +GGDGT HEVVNG
Sbjct: 66  RLIVIGGDGTMHEVVNG 79

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BLAST of Lsi09G015940 vs. ExPASy TrEMBL
Match: A0A0A0KBC3 (DAGKc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006780 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.4e-84
Identity = 158/170 (92.94%), Postives = 160/170 (94.12%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALGPSMATKPFIT AEQPIAPDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT K+WKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGVRDSCIVTILSYLFPLST 171
           DGTFHEVVNGFFWAGKPIVN DGEA KSTALGVRDS IVTILS LFPLST
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGVRDSFIVTILSCLFPLST 171

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BLAST of Lsi09G015940 vs. ExPASy TrEMBL
Match: A0A6J1CZJ4 (sphingoid long-chain bases kinase 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016221 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.9e-76
Identity = 138/153 (90.20%), Postives = 145/153 (94.77%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           MVV FCYH+ALG SMATKPF+TRAEQPIAPDLYADRS+FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 1   MVVCFCYHIALGASMATKPFVTRAEQPIAPDLYADRSVFHRGSSSFSSSSGRRDLVFVVN 60

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKTVKEW KLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGA+AVIAVGG
Sbjct: 61  PRGANGKTVKEWNKLVPYLRTRLSEHYNICESLTSGPCHAIDITREAIREGAEAVIAVGG 120

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKP++N DG A K TALG+
Sbjct: 121 DGTFHEVVNGFFWAGKPVINNDGAARKPTALGL 153

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BLAST of Lsi09G015940 vs. ExPASy TrEMBL
Match: A0A5D3BVA2 (Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001350 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.2e-75
Identity = 141/153 (92.16%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALG SMATKPFIT AEQP APDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT KEWKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKPIVN DGEA KSTALG+
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGI 154

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BLAST of Lsi09G015940 vs. ExPASy TrEMBL
Match: A0A1S3C7U6 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497689 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.2e-75
Identity = 141/153 (92.16%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALG SMATKPFIT AEQP APDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT KEWKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKPIVN DGEA KSTALG+
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGI 154

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BLAST of Lsi09G015940 vs. ExPASy TrEMBL
Match: A0A6J1KFE4 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111494546 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 9.3e-73
Identity = 138/153 (90.20%), Postives = 141/153 (92.16%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           MV  FCYHLA  PSMATKPF+TRAEQPIAPDL ADRSIFHRGSSSF SSSGRRDLVFVVN
Sbjct: 1   MVECFCYHLAFRPSMATKPFVTRAEQPIAPDLNADRSIFHRGSSSF-SSSGRRDLVFVVN 60

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKTVKEWKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVI VGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIPVGG 120

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFW GKP+VN DGE  KSTALG+
Sbjct: 121 DGTFHEVVNGFFWDGKPVVNNDGEVHKSTALGL 152

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BLAST of Lsi09G015940 vs. NCBI nr
Match: XP_038876180.1 (sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 303.1 bits (775), Expect = 1.8e-78
Identity = 146/153 (95.42%), Postives = 148/153 (96.73%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           MVVSFCYHLALGPSMATKPFITRAEQP+APD YADRSIFHRGSSSFSSSSGRRDLVFVVN
Sbjct: 1   MVVSFCYHLALGPSMATKPFITRAEQPMAPDFYADRSIFHRGSSSFSSSSGRRDLVFVVN 60

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKTVKEWKKLVPYLRTRL E YNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEQYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKPIVN DGEA KSTALG+
Sbjct: 121 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGI 153

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BLAST of Lsi09G015940 vs. NCBI nr
Match: XP_031743330.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus] >KAE8646552.1 hypothetical protein Csa_005226 [Cucumis sativus])

HSP 1 Score: 296.6 bits (758), Expect = 1.7e-76
Identity = 142/153 (92.81%), Postives = 145/153 (94.77%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALGPSMATKPFIT AEQPIAPDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT K+WKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKPIVN DGEA KSTALG+
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGI 154

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BLAST of Lsi09G015940 vs. NCBI nr
Match: XP_022147219.1 (sphingoid long-chain bases kinase 2, mitochondrial [Momordica charantia] >XP_022147220.1 sphingoid long-chain bases kinase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 293.5 bits (750), Expect = 1.4e-75
Identity = 138/153 (90.20%), Postives = 145/153 (94.77%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           MVV FCYH+ALG SMATKPF+TRAEQPIAPDLYADRS+FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 1   MVVCFCYHIALGASMATKPFVTRAEQPIAPDLYADRSVFHRGSSSFSSSSGRRDLVFVVN 60

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKTVKEW KLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGA+AVIAVGG
Sbjct: 61  PRGANGKTVKEWNKLVPYLRTRLSEHYNICESLTSGPCHAIDITREAIREGAEAVIAVGG 120

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKP++N DG A K TALG+
Sbjct: 121 DGTFHEVVNGFFWAGKPVINNDGAARKPTALGL 153

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BLAST of Lsi09G015940 vs. NCBI nr
Match: XP_008458171.1 (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis melo] >TYK03040.1 sphingoid long-chain bases kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 292.7 bits (748), Expect = 2.4e-75
Identity = 141/153 (92.16%), Postives = 143/153 (93.46%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALG SMATKPFIT AEQP APDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT KEWKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFWAGKPIVN DGEA KSTALG+
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGI 154

[Close]

BLAST of Lsi09G015940 vs. NCBI nr
Match: XP_023000266.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 283.1 bits (723), Expect = 1.9e-72
Identity = 138/153 (90.20%), Postives = 141/153 (92.16%), Query Frame = 0


Query: 1   MVVSFCYHLALGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           MV  FCYHLA  PSMATKPF+TRAEQPIAPDL ADRSIFHRGSSSF SSSGRRDLVFVVN
Sbjct: 1   MVECFCYHLAFRPSMATKPFVTRAEQPIAPDLNADRSIFHRGSSSF-SSSGRRDLVFVVN 60

Query: 61  PRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKTVKEWKKLVPYLRTRL EHYNICESLTSGPCHAIDITREAIREGADAVI VGG
Sbjct: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIPVGG 120

Query: 121 DGTFHEVVNGFFWAGKPIVNKDGEACKSTALGV 154
           DGTFHEVVNGFFW GKP+VN DGE  KSTALG+
Sbjct: 121 DGTFHEVVNGFFWDGKPVVNNDGEVHKSTALGL 152

[Close]

BLAST of Lsi09G015940 vs. TAIR 10
Match: AT2G46090.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 177.2 bits (448), Expect = 1.4e-44
Identity = 89/143 (62.24%), Postives = 106/143 (74.13%), Query Frame = 0


Query: 11  LGPSMATKPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVK 70
           L P  A +P   R +Q I  D +  R      +++  SSSG RDLVFVVNP+GANG+T K
Sbjct: 10  LCPFTAKQPSFLRKQQRITSDRFTFRGGGGGDAAAVVSSSGLRDLVFVVNPQGANGRTAK 69

Query: 71  EWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNG 130
           EWKKL+P+LR+RL +  N+ E LTSGP HAIDITREAIR+GADAVIAVGGDGT HEVVNG
Sbjct: 70  EWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVVNG 129

Query: 131 FFWAGKPIVNKDGEACKSTALGV 154
           FFW GKP+    GEA +STALG+
Sbjct: 130 FFWEGKPVGYLSGEASRSTALGL 152

[Close]

BLAST of Lsi09G015940 vs. TAIR 10
Match: AT5G23450.1 (long-chain base (LCB) kinase 1 )

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-06
Identity = 33/133 (24.81%), Postives = 62/133 (46.62%), Query Frame = 0


Query: 55  LVFVVNPRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADA 114
           ++ ++NPR  +G+++K +  +V  +         + ++  +G    +  T + I   +D 
Sbjct: 250 MLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVD-INLCSDG 309

Query: 115 VIAVGGDGTFHEVVNGFFWAGKPIVNKDGEACK--STALGVRDSCIVTILSYLFPLSTIT 174
           +I VGGDG  +EV+NG      P   K+G +        G  +S + T+L    P+S   
Sbjct: 310 IICVGGDGIINEVLNGLLTRSNP---KEGVSIPIGIVPAGSDNSLVWTVLGVRDPISAAL 369

Query: 175 GSYQSVLTRSDYF 186
              +  LT +D F
Sbjct: 370 SIVKGGLTATDVF 378

[Close]

BLAST of Lsi09G015940 vs. TAIR 10
Match: AT5G23450.2 (long-chain base (LCB) kinase 1 )

HSP 1 Score: 48.5 bits (114), Expect = 7.4e-06
Identity = 33/133 (24.81%), Postives = 62/133 (46.62%), Query Frame = 0


Query: 55  LVFVVNPRGANGKTVKEWKKLVPYLRTRLDEHYNICESLTSGPCHAIDITREAIREGADA 114
           ++ ++NPR  +G+++K +  +V  +         + ++  +G    +  T + I   +D 
Sbjct: 250 MLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVD-INLCSDG 309

Query: 115 VIAVGGDGTFHEVVNGFFWAGKPIVNKDGEACK--STALGVRDSCIVTILSYLFPLSTIT 174
           +I VGGDG  +EV+NG      P   K+G +        G  +S + T+L    P+S   
Sbjct: 310 IICVGGDGIINEVLNGLLTRSNP---KEGVSIPIGIVPAGSDNSLVWTVLGVRDPISAAL 369

Query: 175 GSYQSVLTRSDYF 186
              +  LT +D F
Sbjct: 370 SIVKGGLTATDVF 378

[Close]

BLAST of Lsi09G015940 vs. TAIR 10
Match: AT5G51290.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 42.4 bits (98), Expect = 5.3e-04
Identity = 25/83 (30.12%), Postives = 43/83 (51.81%), Query Frame = 0


Query: 53  RDLVFVVNPRGANGKTVKEWKKLVP-YLRTRLDEHYNICESLTSGPCHAIDITREAIREG 112
           R+L+  V+P+   G   K W+ +   ++R ++    N    +T    HA D+      + 
Sbjct: 163 RNLLVFVHPKSGKGNGSKVWETVSKIFIRAKV----NTKVIVTERAGHAFDVMASIQNKE 222

Query: 113 A---DAVIAVGGDGTFHEVVNGF 132
               D +IAVGGDG F+E++NG+
Sbjct: 223 LHTYDGIIAVGGDGFFNEILNGY 241

The following BLAST results are available for this feature:

BLAST of Lsi09G015940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82359	1.9e-43	62.24	Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=37...	[more]
O14159	9.4e-06	30.00	Sphingoid long chain base kinase 4 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]
Q9LRB0	1.0e-04	24.81	Sphingoid long-chain bases kinase 1 OS=Arabidopsis thaliana OX=3702 GN=LCBK1 PE=...	[more]
O34799	1.8e-04	37.66	Putative lipid kinase YtlR OS=Bacillus subtilis (strain 168) OX=224308 GN=ytlR P...	[more]

BLAST of Lsi09G015940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBC3	2.4e-84	92.94	DAGKc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006780 PE=4 ...	[more]
A0A6J1CZJ4	6.9e-76	90.20	sphingoid long-chain bases kinase 2, mitochondrial OS=Momordica charantia OX=367...	[more]
A0A5D3BVA2	1.2e-75	92.16	Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3C7U6	1.2e-75	92.16	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1KFE4	9.3e-73	90.20	sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 G...	[more]

BLAST of Lsi09G015940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038876180.1	1.8e-78	95.42	sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida]	[more]
XP_031743330.1	1.7e-76	92.81	sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus] >KAE8646552...	[more]
XP_022147219.1	1.4e-75	90.20	sphingoid long-chain bases kinase 2, mitochondrial [Momordica charantia] >XP_022...	[more]
XP_008458171.1	2.4e-75	92.16	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis melo] >TY...	[more]
XP_023000266.1	1.9e-72	90.20	sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita maxima]	[more]

BLAST of Lsi09G015940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46090.1	1.4e-44	62.24	Diacylglycerol kinase family protein	[more]
AT5G23450.1	7.4e-06	24.81	long-chain base (LCB) kinase 1	[more]
AT5G23450.2	7.4e-06	24.81	long-chain base (LCB) kinase 1	[more]
AT5G51290.1	5.3e-04	30.12	Diacylglycerol kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 55..133 e-value: 4.6E-15 score: 55.4
IPR001206	Diacylglycerol kinase, catalytic domain	PROSITE	PS50146	DAGK	coord: 50..130 score: 19.113461
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, N-terminal	GENE3D	3.40.50.10330		coord: 36..154 e-value: 6.7E-20 score: 72.6
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 42..155
None	No IPR available	PANTHER	PTHR12358:SF94	BNAA04G26670D PROTEIN	coord: 42..155
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 53..138

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G015940.1	Lsi09G015940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0003951	NAD+ kinase activity
molecular_function	GO:0016301	kinase activity