Lsi09G015720 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G015720
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncytochrome P450 86A8-like
Locationchr09: 23852959 .. 23854614 (-)
RNA-Seq ExpressionLsi09G015720
SyntenyLsi09G015720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGTGGGATTCGCGTTAGTTATAGTAACGGCAGTTACGGCGTATCTTCTTTGGTTTACTTTCATTTCACGGTCGTTAAAAGGTCCTCAAATGTGGCCGTTGCTCGGCAGCCTCCCTGGCTTGATCGAAAATTGCGACCGTTTACATGACTGGATCTCCGACAACCTCCGCGCGTGTGGCGGCACGTATCAAACCTGCATCTGGGCTTTCCCTTTCTTCGCTAAAAAGCAAGGCCTCGTGACTGTCACGTGTGACCCGAAAAATCTCGAGCATATTCTCAAGACCCGATTCGATAATTACCCTAAAGGCCCCACTTGGCAAGCTGTTTTTCACGAGTTACTTGGACAAGGCATTTTTAACTCCGACGGTGACACGTGGCTTTTCCAGAGAAAAACTGCTGCTTTGGAATTTACTACTAGGACCCTTCGTCAAGCCATGGCTCGATGGGTTAGCCGAGCTATTAAGTTGAGGTTTTGTCCTATTCTTAAACAAGCCGAGCTGAATTCTGAACCGATTGATCTTCAGGATGTGTTGTTACGTCTTACTTTTGATAATATTTGTGGCTTGGCTTTTGGTAAAGATCCGATGACTTGCGCGCCGGGTTTCCCTGAAAATGGCTTTGCTATGGCTTTCGATCGAGCCACCGAGGCTTCATTGCAACGGTTTATTCTACCGGAAGTGCTTTGGAAATTCAAAAAGTGGCTTCGGCTTGGAATGGAAGTCAGCTTGAGCCGGAGCCTAGTTCATATTGAGGAGTATTTATCCAGTGTGATTGATTCACGTAAGCTCGAGTTGCTAAATCAGCCTAAAGACTCCACCCCACATGATGATTTATTGTCACGTTTCATGAAGAAAAAGGAAGGTAATTACTCTAACGCGTTCCTTCGCCACGTGGCGCTTAATTTCATCTTAGCTGGACGGGACACGTCATCCGTTGCGCTGAGCTGGTTCTTCTGGTTGGTAACATTAAACCCACTCATTGAAGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGAAATAACATCCAATCATGGCTCGAGGAGCCGTTAGGATTCGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTAAGATTGTACCCATCAGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACGTTCTTCCGGACGGAACCTTCGTTCCGGCGGGATCGTCAGTGACTTATTCGATCTATTCCACCGGAAGAATGAAGTCGACGTGGGGAGAAGACTGCCTCGAATTCCGGCCGGAGCGGTGGTTGACGGCGGACGGAAAGAAATTCATACCGTACGATTCGTACAAATTCGTAGCGTTCAATGCAGGGCCGAGGATCTGCTTAGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATAGCGGCAGCGTTGCTACTCCGGCACCGGCTGACGGTGGCAGCAGGGCATAAGGTGGAGCAAAAAATGTCGTTGACGCTGTTTATGAAGTATGGGCTGAAGGTGAACGTTCACCGGAGGGACTTGGAAGGAATTGTGGAAAGTATCAGGGGCAAATACGGAAATTCGGAAGGGAAAGATGTTCCGTTAGTCGCCGTTGTGAACTGTAACGGCGGGTGTAACGGATTTAACGAAGTACCATGCTAA

mRNA sequence

ATGGACGTGGGATTCGCGTTAGTTATAGTAACGGCAGTTACGGCGTATCTTCTTTGGTTTACTTTCATTTCACGGTCGTTAAAAGGTCCTCAAATGTGGCCGTTGCTCGGCAGCCTCCCTGGCTTGATCGAAAATTGCGACCGTTTACATGACTGGATCTCCGACAACCTCCGCGCGTGTGGCGGCACGTATCAAACCTGCATCTGGGCTTTCCCTTTCTTCGCTAAAAAGCAAGGCCTCGTGACTGTCACGTGTGACCCGAAAAATCTCGAGCATATTCTCAAGACCCGATTCGATAATTACCCTAAAGGCCCCACTTGGCAAGCTGTTTTTCACGAGTTACTTGGACAAGGCATTTTTAACTCCGACGGTGACACGTGGCTTTTCCAGAGAAAAACTGCTGCTTTGGAATTTACTACTAGGACCCTTCGTCAAGCCATGGCTCGATGGGTTAGCCGAGCTATTAAGTTGAGGTTTTGTCCTATTCTTAAACAAGCCGAGCTGAATTCTGAACCGATTGATCTTCAGGATGTGTTGTTACGTCTTACTTTTGATAATATTTGTGGCTTGGCTTTTGGTAAAGATCCGATGACTTGCGCGCCGGGTTTCCCTGAAAATGGCTTTGCTATGGCTTTCGATCGAGCCACCGAGGCTTCATTGCAACGGTTTATTCTACCGGAAGTGCTTTGGAAATTCAAAAAGTGGCTTCGGCTTGGAATGGAAGTCAGCTTGAGCCGGAGCCTAGTTCATATTGAGGAGTATTTATCCAGTGTGATTGATTCACGTAAGCTCGAGTTGCTAAATCAGCCTAAAGACTCCACCCCACATGATGATTTATTGTCACGTTTCATGAAGAAAAAGGAAGGTAATTACTCTAACGCGTTCCTTCGCCACGTGGCGCTTAATTTCATCTTAGCTGGACGGGACACGTCATCCGTTGCGCTGAGCTGGTTCTTCTGGTTGGTAACATTAAACCCACTCATTGAAGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGAAATAACATCCAATCATGGCTCGAGGAGCCGTTAGGATTCGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTAAGATTGTACCCATCAGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACGTTCTTCCGGACGGAACCTTCGTTCCGGCGGGATCGTCAGTGACTTATTCGATCTATTCCACCGGAAGAATGAAGTCGACGTGGGGAGAAGACTGCCTCGAATTCCGGCCGGAGCGGTGGTTGACGGCGGACGGAAAGAAATTCATACCGTACGATTCGTACAAATTCGTAGCGTTCAATGCAGGGCCGAGGATCTGCTTAGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATAGCGGCAGCGTTGCTACTCCGGCACCGGCTGACGGTGGCAGCAGGGCATAAGGTGGAGCAAAAAATGTCGTTGACGCTGTTTATGAAGTATGGGCTGAAGGTGAACGTTCACCGGAGGGACTTGGAAGGAATTGTGGAAAGTATCAGGGGCAAATACGGAAATTCGGAAGGGAAAGATGTTCCGTTAGTCGCCGTTGTGAACTGTAACGGCGGGTGTAACGGATTTAACGAAGTACCATGCTAA

Coding sequence (CDS)

ATGGACGTGGGATTCGCGTTAGTTATAGTAACGGCAGTTACGGCGTATCTTCTTTGGTTTACTTTCATTTCACGGTCGTTAAAAGGTCCTCAAATGTGGCCGTTGCTCGGCAGCCTCCCTGGCTTGATCGAAAATTGCGACCGTTTACATGACTGGATCTCCGACAACCTCCGCGCGTGTGGCGGCACGTATCAAACCTGCATCTGGGCTTTCCCTTTCTTCGCTAAAAAGCAAGGCCTCGTGACTGTCACGTGTGACCCGAAAAATCTCGAGCATATTCTCAAGACCCGATTCGATAATTACCCTAAAGGCCCCACTTGGCAAGCTGTTTTTCACGAGTTACTTGGACAAGGCATTTTTAACTCCGACGGTGACACGTGGCTTTTCCAGAGAAAAACTGCTGCTTTGGAATTTACTACTAGGACCCTTCGTCAAGCCATGGCTCGATGGGTTAGCCGAGCTATTAAGTTGAGGTTTTGTCCTATTCTTAAACAAGCCGAGCTGAATTCTGAACCGATTGATCTTCAGGATGTGTTGTTACGTCTTACTTTTGATAATATTTGTGGCTTGGCTTTTGGTAAAGATCCGATGACTTGCGCGCCGGGTTTCCCTGAAAATGGCTTTGCTATGGCTTTCGATCGAGCCACCGAGGCTTCATTGCAACGGTTTATTCTACCGGAAGTGCTTTGGAAATTCAAAAAGTGGCTTCGGCTTGGAATGGAAGTCAGCTTGAGCCGGAGCCTAGTTCATATTGAGGAGTATTTATCCAGTGTGATTGATTCACGTAAGCTCGAGTTGCTAAATCAGCCTAAAGACTCCACCCCACATGATGATTTATTGTCACGTTTCATGAAGAAAAAGGAAGGTAATTACTCTAACGCGTTCCTTCGCCACGTGGCGCTTAATTTCATCTTAGCTGGACGGGACACGTCATCCGTTGCGCTGAGCTGGTTCTTCTGGTTGGTAACATTAAACCCACTCATTGAAGAAAAAATCCTCCGCGAGATCTGCACCGTTCTGATTGAGACACGTGGAAATAACATCCAATCATGGCTCGAGGAGCCGTTAGGATTCGAAGAGATCGACCGTCTGATTTATCTCAAAGCCGCTCTCACCGAAACCCTAAGATTGTACCCATCAGTGCCGGAGGATTCAAAGCACGTCGTCGCCGACGACGTTCTTCCGGACGGAACCTTCGTTCCGGCGGGATCGTCAGTGACTTATTCGATCTATTCCACCGGAAGAATGAAGTCGACGTGGGGAGAAGACTGCCTCGAATTCCGGCCGGAGCGGTGGTTGACGGCGGACGGAAAGAAATTCATACCGTACGATTCGTACAAATTCGTAGCGTTCAATGCAGGGCCGAGGATCTGCTTAGGGAAGGATTTGGCGTATTTACAGATGAAGTCGATAGCGGCAGCGTTGCTACTCCGGCACCGGCTGACGGTGGCAGCAGGGCATAAGGTGGAGCAAAAAATGTCGTTGACGCTGTTTATGAAGTATGGGCTGAAGGTGAACGTTCACCGGAGGGACTTGGAAGGAATTGTGGAAAGTATCAGGGGCAAATACGGAAATTCGGAAGGGAAAGATGTTCCGTTAGTCGCCGTTGTGAACTGTAACGGCGGGTGTAACGGATTTAACGAAGTACCATGCTAA

Protein sequence

MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRACGGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLLRLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGMEVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVALNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCNGGCNGFNEVPC
Homology
BLAST of Lsi09G015720 vs. ExPASy Swiss-Prot
Match: O80823 (Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 5.1e-248
Identity = 408/521 (78.31%), Postives = 463/521 (88.87%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  AL+I++A+TAY LW TFISR LKGP++WP+LGSLPGLIENC+R+HDWISDNLRAC
Sbjct: 1   MEISTALMILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
            GTYQTCI A PF AKKQGLVTVTCDP+NLEHILK RFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  SGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWV+RAIKLRF PIL+ A L SEPIDLQD+LL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP TCAPG P N FA+AFDRATEASLQRFILPE+LWKFK+WLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSL+RSLV ++ YLS +I +RK E++ Q  +   HDDLLSRF+KKKE +YS+  L+ VA
Sbjct: 241 EVSLTRSLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKE-SYSDETLQRVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWL+T +P IE+KILREICTVL+ETRG+++  W +EPL  EE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           +DRL++LKAAL+ETLRLYPSVPEDSK  V DDVLPDGTFVPAGSS+TYSIYS GRMKSTW
Sbjct: 361 LDRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTW 420

Query: 421 GEDCLEFRPERWLT-ADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRH 480
           GEDCLEF+PERW++ +DG +FI +D +KFVAFNAGPRICLGKDLAYLQMKSIA+A+LLRH
Sbjct: 421 GEDCLEFKPERWISQSDGGRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIR 521
           RLTV  GHKVEQKMSLTLFMKYGL VNVH RDL  I   +R
Sbjct: 481 RLTVVTGHKVEQKMSLTLFMKYGLLVNVHERDLTAIAADLR 520

BLAST of Lsi09G015720 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 1.1e-247
Identity = 414/546 (75.82%), Postives = 469/546 (85.90%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDV   +++V  V AY LWF  ISR LKGP++WP+LGSLPGLIE  DR+HDWI++NLRAC
Sbjct: 1   MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI A PF AKKQGLVTVTCDPKN+EH+LKTRFDNYPKGPTWQAVFH+ LGQGIF
Sbjct: 61  GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL+ A+ N EP+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFILPE LW+ KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTP-HDDLLSRFMKKKEGNYSNAFLRHV 300
           EVSLSRSL  I+ YL +VI++RK ELL+Q +     HDDLLSRFMKKK+ +YS  FLRHV
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV 300

Query: 301 ALNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFE 360
           ALNFILAGRDTSSVALSWFFWL+T +P +E+KI+REIC+VLIETRG ++ SW  EPL F+
Sbjct: 301 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 360

Query: 361 EIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKST 420
           E+DRL+YLKAAL+ETLRLYPSVPEDSKHVV DD+LPDGTFVPAGSSVTYSIY+ GRMKST
Sbjct: 361 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 420

Query: 421 WGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRH 480
           WGEDCLEF+PERW++ D  KF+ +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAA+LLRH
Sbjct: 421 WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNC 540
           RLTVA GHKVEQKMSLTLFMK GL VNVH+RDLE +++S+  K  N          VV  
Sbjct: 481 RLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERND---------VVVL 537

Query: 541 NGGCNG 546
           NG CNG
Sbjct: 541 NGKCNG 537

BLAST of Lsi09G015720 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 1.2e-244
Identity = 410/548 (74.82%), Postives = 475/548 (86.68%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  A+++V  VT Y LWF  ISR LKGP++WPLLGSLPGLIE  DR+H+WI++NLRAC
Sbjct: 1   MEISNAMLLVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+A PF AKKQGLVTVTCDPKNLEH+LKTRFDNYPKGPTWQ+VFH+LLGQGIF
Sbjct: 61  GGTYQTCIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL  A+ N+EP+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFI+P+ +WK KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDST--PHDDLLSRFMKKKEGNYSNAFLRH 300
           EVSLSRSL  I+EYL++VI++RK EL++Q +  T   HDDLLSRFM KK  +YS+ FL+H
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTESYSDTFLQH 300

Query: 301 VALNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRG-NNIQSWLEEPLG 360
           VALNFILAGRDTSSVALSWFFWL+T++P +E+KI+REIC+VLIETRG +++ SW EEPLG
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMK 420
           F+EIDRL+YLKAA++ETLRLYPSVPEDSKHV  DDVLPDGTFVPAGSSVTYSIY+ GRMK
Sbjct: 361 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLL 480
           STWGEDCLEF PERW++    KFI +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAA+LL
Sbjct: 421 STWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLL 480

Query: 481 RHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVV 540
           RHRLTV  GHKVEQKMSLTLFMK GL VN+++RDL+GI++S+  K   S+G         
Sbjct: 481 RHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKRDLQGIIKSLVVK--KSDGVS------- 537

Query: 541 NCNGGCNG 546
             NG CNG
Sbjct: 541 --NGQCNG 537

BLAST of Lsi09G015720 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 2.3e-232
Identity = 391/530 (73.77%), Postives = 452/530 (85.28%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQ---MWPLLGSLPGLIENCDRLHDWISDNL 60
           M+V   ++IV  V AYLLWF  I++S+KGP+   MWP++GSLPGLIEN +R+H+WI+DNL
Sbjct: 1   MEVSTTMMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLIENGNRMHEWIADNL 60

Query: 61  RACGGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQ 120
           RAC GTYQTCI A PF A+KQ LVTVTCDPKNLEHILK RFDNYPKGPTWQAVFH+LLG+
Sbjct: 61  RACSGTYQTCICAIPFLARKQ-LVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGE 120

Query: 121 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQD 180
           GIFNSDGDTWLFQRKTAALEFTTRTLRQAM RWV+RAIK RFCPIL+ A++  +P+DLQD
Sbjct: 121 GIFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQD 180

Query: 181 VLLRLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLR 240
           +LLRLTFDNICGLAFGKDP T +P  PEN FA +FDRATEA+L RFI+PE +WK KK L 
Sbjct: 181 LLLRLTFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLG 240

Query: 241 LGMEVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLR 300
           LG+EVSL+ SL  ++ Y++ VI++RKLELLN       HDDLLSRFMKKKE +YS+ FL+
Sbjct: 241 LGLEVSLNNSLKQVDNYMTDVINTRKLELLNHQNGGPQHDDLLSRFMKKKE-SYSDKFLQ 300

Query: 301 HVALNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLG 360
           HVALNFILAGRDTSSVALSWFFWLV+ NP +EEKIL EICT+L ETRGN+   WLEEPL 
Sbjct: 301 HVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLV 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMK 420
           FEE+D+L+YLKAAL+ETLRLYPSVPEDSKHV++DD LPDGTFVPAGS++TYSIYSTGRMK
Sbjct: 361 FEEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLL 480
             WGEDCLEF+PERW++ DG KF   D+++FVAFNAGPRICLGKDLAYLQMKSIAAA+LL
Sbjct: 421 FIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAVLL 480

Query: 481 RHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSE 528
           RHRL VA GHKVEQKMSLTLFMK GL +NV  RDL  I+  I  K+G  E
Sbjct: 481 RHRLAVAPGHKVEQKMSLTLFMKDGLVMNVTPRDLTPILAKIE-KFGKVE 527

BLAST of Lsi09G015720 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 1.8e-229
Identity = 378/519 (72.83%), Postives = 444/519 (85.55%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MD   A +I+T +  Y++WF  + RS KGP++WPL+GSLP LI N  R+HD+I+DNLR C
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+  PF AKKQG VTVTCDPKNLEHILKTRFDNYPKGP+WQ+VFH+LLG GIF
Sbjct: 61  GGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTW FQRKTAALEFTTRTLRQAMARWV RAIK R  PIL+ A   +EPIDLQDVLL
Sbjct: 121 NSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP T +P FPENGFA+AFD ATEA+LQRFI+PE +WK +KWLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           E  +SRS+ H++ YLS +I++RKLELL Q +D + HDDLLSRFMKKKE +YS+ +L++VA
Sbjct: 241 EDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKE-SYSDKYLKYVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVA+SWFFWLV+LNP +EEKI+ EICT+LI+TR  N+  W +EPL F+E
Sbjct: 301 LNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           ID+L+YLKAAL+ETLRLYPSVPEDSK VVA+DVLPDGTFVP+GS+VTYSIYS GRMK  W
Sbjct: 361 IDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEF+PERWL  +  +    + YKFVAFNAGPRICLGKDLAYLQMKSI A++LLRHR
Sbjct: 421 GEDCLEFKPERWL--EESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESI 520
           LTVA GH+VEQKMSLTLFMK+GLK++VH+RDL   VE +
Sbjct: 481 LTVAPGHRVEQKMSLTLFMKFGLKMDVHKRDLTLPVEKV 516

BLAST of Lsi09G015720 vs. ExPASy TrEMBL
Match: A0A5D3BVJ6 (Cytochrome P450 86A8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001130 PE=3 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 529/549 (96.36%), Postives = 542/549 (98.72%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFAKKQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKK+GNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVL+ETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVH+R+LEG+VES+RGKYGNS GKD PL+AVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHKRNLEGMVESMRGKYGNSHGKDGPLIAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNG  EV
Sbjct: 541 GGCNGVKEV 549

BLAST of Lsi09G015720 vs. ExPASy TrEMBL
Match: A0A1S3C7C5 (cytochrome P450 86A8-like OS=Cucumis melo OX=3656 GN=LOC103497711 PE=3 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 529/549 (96.36%), Postives = 542/549 (98.72%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFAKKQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKK+GNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVL+ETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVH+R+LEG+VES+RGKYGNS GKD PL+AVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHKRNLEGMVESMRGKYGNSHGKDGPLIAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNG  EV
Sbjct: 541 GGCNGVKEV 549

BLAST of Lsi09G015720 vs. ExPASy TrEMBL
Match: A0A6J1H676 (cytochrome P450 86A8 OS=Cucurbita moschata OX=3662 GN=LOC111459971 PE=3 SV=1)

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 530/549 (96.54%), Postives = 538/549 (98.00%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG  LVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQ KDSTPHDDLLSRFMKKKE NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP IEEKILREICTVLIETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVESIRGK GNS+G D PLVAVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKNGNSQGNDTPLVAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNGFN+V
Sbjct: 541 GGCNGFNDV 549

BLAST of Lsi09G015720 vs. ExPASy TrEMBL
Match: A0A6J1KW55 (cytochrome P450 86A8 OS=Cucurbita maxima OX=3661 GN=LOC111499241 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 529/549 (96.36%), Postives = 539/549 (98.18%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFP+FA KQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPYFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQ KDSTPHDDLLSRFMKKKE NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP IEEKILREICTVLIETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVE +RGK GNS+GKD PLVAVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVEILRGKNGNSQGKDTPLVAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNGFN+V
Sbjct: 541 GGCNGFNDV 549

BLAST of Lsi09G015720 vs. ExPASy TrEMBL
Match: A0A0A0K9Z1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004550 PE=3 SV=1)

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 525/545 (96.33%), Postives = 538/545 (98.72%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFAKKQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKK+GNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWL+EPL FEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLDEPLEFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADG KFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGTKFIAYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVN+H+R+LEGIVES+RGKYGNS+GKD PL+ VVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNIHKRNLEGIVESMRGKYGNSQGKDSPLITVVNCN 540

Query: 541 GGCNG 546
           GGCNG
Sbjct: 541 GGCNG 545

BLAST of Lsi09G015720 vs. NCBI nr
Match: XP_038907189.1 (cytochrome P450 86A8-like [Benincasa hispida])

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 537/549 (97.81%), Postives = 540/549 (98.36%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGFALVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 23  MDVGFALVIVAAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 82

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFAKKQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 83  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 142

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAE NSEPIDLQDVLL
Sbjct: 143 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAEFNSEPIDLQDVLL 202

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 203 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 262

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSL RSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA
Sbjct: 263 EVSLGRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 322

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPL FEE
Sbjct: 323 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLAFEE 382

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 383 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 442

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 443 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 502

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNS+ KD PLV VVN N
Sbjct: 503 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSQEKDTPLVPVVNGN 562

Query: 541 GGCNGFNEV 550
           GGCNGFNEV
Sbjct: 563 GGCNGFNEV 571

BLAST of Lsi09G015720 vs. NCBI nr
Match: XP_008458223.1 (PREDICTED: cytochrome P450 86A8-like [Cucumis melo] >TYK03018.1 cytochrome P450 86A8-like [Cucumis melo var. makuwa])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 529/549 (96.36%), Postives = 542/549 (98.72%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFAKKQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILK+AELNS PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKEAELNSHPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKK+GNYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKDGNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVL+ETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLLETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFI YD YKFV+FNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIQYDPYKFVSFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVH+R+LEG+VES+RGKYGNS GKD PL+AVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHKRNLEGMVESMRGKYGNSHGKDGPLIAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNG  EV
Sbjct: 541 GGCNGVKEV 549

BLAST of Lsi09G015720 vs. NCBI nr
Match: XP_023547593.1 (cytochrome P450 86A8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 531/549 (96.72%), Postives = 539/549 (98.18%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQ KDSTPHDDLLSRFMKKKE NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP IEEKILREICTVLIETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVESIRGK GNS+G D PLVAVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKKGNSQGNDTPLVAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNGFN+V
Sbjct: 541 GGCNGFNDV 549

BLAST of Lsi09G015720 vs. NCBI nr
Match: XP_022958814.1 (cytochrome P450 86A8 [Cucurbita moschata])

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 530/549 (96.54%), Postives = 538/549 (98.00%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG  LVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLGLVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFPFFA KQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPFFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQ KDSTPHDDLLSRFMKKKE NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP IEEKILREICTVLIETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVESIRGK GNS+G D PLVAVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVESIRGKNGNSQGNDTPLVAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNGFN+V
Sbjct: 541 GGCNGFNDV 549

BLAST of Lsi09G015720 vs. NCBI nr
Match: XP_023006552.1 (cytochrome P450 86A8 [Cucurbita maxima])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 529/549 (96.36%), Postives = 539/549 (98.18%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDVG ALVIV AVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC
Sbjct: 1   MDVGLALVIVMAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCIWAFP+FA KQGLVTVTCDP+NLEHILKTRFDNYPKGPTWQAVFHELLGQGIF
Sbjct: 61  GGTYQTCIWAFPYFATKQGLVTVTCDPRNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNS+PIDLQDVLL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSQPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM
Sbjct: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSLSRSLVHIEEYLSSVIDSRKLELLNQ KDSTPHDDLLSRFMKKKE NYSNAFLRHVA
Sbjct: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQLKDSTPHDDLLSRFMKKKESNYSNAFLRHVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWLVTLNP IEEKILREICTVLIETRGNNIQSWL+EPLGFEE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLVTLNPQIEEKILREICTVLIETRGNNIQSWLDEPLGFEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           IDRLIYLKAALTETLRLYPSVPEDSKHVVADD+LPDGTFVPAGSSVTYSIYSTGRMKSTW
Sbjct: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDILPDGTFVPAGSSVTYSIYSTGRMKSTW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEFRPERWLTADGKKFIPYD YKFVAFNAGPRICLGKDLAYLQMKSIAAA+LLRHR
Sbjct: 421 GEDCLEFRPERWLTADGKKFIPYDPYKFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNCN 540
           LTVAAGHKVEQKMSLTLFMKYGLKVNVHRR+LEGIVE +RGK GNS+GKD PLVAVVNCN
Sbjct: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRELEGIVEILRGKNGNSQGKDTPLVAVVNCN 540

Query: 541 GGCNGFNEV 550
           GGCNGFN+V
Sbjct: 541 GGCNGFNDV 549

BLAST of Lsi09G015720 vs. TAIR 10
Match: AT2G45970.1 (cytochrome P450, family 86, subfamily A, polypeptide 8 )

HSP 1 Score: 858.2 bits (2216), Expect = 3.6e-249
Identity = 408/521 (78.31%), Postives = 463/521 (88.87%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  AL+I++A+TAY LW TFISR LKGP++WP+LGSLPGLIENC+R+HDWISDNLRAC
Sbjct: 1   MEISTALMILSAITAYFLWLTFISRCLKGPRVWPILGSLPGLIENCERMHDWISDNLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
            GTYQTCI A PF AKKQGLVTVTCDP+NLEHILK RFDNYPKGPTWQAVFH+LLGQGIF
Sbjct: 61  SGTYQTCICAIPFLAKKQGLVTVTCDPRNLEHILKNRFDNYPKGPTWQAVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAMARWV+RAIKLRF PIL+ A L SEPIDLQD+LL
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVNRAIKLRFLPILENARLGSEPIDLQDLLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP TCAPG P N FA+AFDRATEASLQRFILPE+LWKFK+WLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTCAPGLPVNTFAVAFDRATEASLQRFILPEILWKFKRWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           EVSL+RSLV ++ YLS +I +RK E++ Q  +   HDDLLSRF+KKKE +YS+  L+ VA
Sbjct: 241 EVSLTRSLVQVDNYLSEIITTRKEEMMTQHNNGKHHDDLLSRFIKKKE-SYSDETLQRVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVALSWFFWL+T +P IE+KILREICTVL+ETRG+++  W +EPL  EE
Sbjct: 301 LNFILAGRDTSSVALSWFFWLITQHPAIEDKILREICTVLVETRGDDVALWTDEPLSCEE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           +DRL++LKAAL+ETLRLYPSVPEDSK  V DDVLPDGTFVPAGSS+TYSIYS GRMKSTW
Sbjct: 361 LDRLVFLKAALSETLRLYPSVPEDSKRAVKDDVLPDGTFVPAGSSITYSIYSAGRMKSTW 420

Query: 421 GEDCLEFRPERWLT-ADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRH 480
           GEDCLEF+PERW++ +DG +FI +D +KFVAFNAGPRICLGKDLAYLQMKSIA+A+LLRH
Sbjct: 421 GEDCLEFKPERWISQSDGGRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIR 521
           RLTV  GHKVEQKMSLTLFMKYGL VNVH RDL  I   +R
Sbjct: 481 RLTVVTGHKVEQKMSLTLFMKYGLLVNVHERDLTAIAADLR 520

BLAST of Lsi09G015720 vs. TAIR 10
Match: AT4G00360.1 (cytochrome P450, family 86, subfamily A, polypeptide 2 )

HSP 1 Score: 857.1 bits (2213), Expect = 8.0e-249
Identity = 414/546 (75.82%), Postives = 469/546 (85.90%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MDV   +++V  V AY LWF  ISR LKGP++WP+LGSLPGLIE  DR+HDWI++NLRAC
Sbjct: 1   MDVSNTMLLVAVVAAYWLWFQRISRWLKGPRVWPVLGSLPGLIEQRDRMHDWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI A PF AKKQGLVTVTCDPKN+EH+LKTRFDNYPKGPTWQAVFH+ LGQGIF
Sbjct: 61  GGTYQTCICAVPFLAKKQGLVTVTCDPKNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL+ A+ N EP+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILETAQNNYEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFILPE LW+ KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFILPEFLWRLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTP-HDDLLSRFMKKKEGNYSNAFLRHV 300
           EVSLSRSL  I+ YL +VI++RK ELL+Q +     HDDLLSRFMKKK+ +YS  FLRHV
Sbjct: 241 EVSLSRSLGEIDGYLDAVINTRKQELLSQRESGVQRHDDLLSRFMKKKDQSYSETFLRHV 300

Query: 301 ALNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFE 360
           ALNFILAGRDTSSVALSWFFWL+T +P +E+KI+REIC+VLIETRG ++ SW  EPL F+
Sbjct: 301 ALNFILAGRDTSSVALSWFFWLITTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEFD 360

Query: 361 EIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKST 420
           E+DRL+YLKAAL+ETLRLYPSVPEDSKHVV DD+LPDGTFVPAGSSVTYSIY+ GRMKST
Sbjct: 361 EVDRLVYLKAALSETLRLYPSVPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKST 420

Query: 421 WGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRH 480
           WGEDCLEF+PERW++ D  KF+ +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAA+LLRH
Sbjct: 421 WGEDCLEFKPERWISPDDGKFVNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRH 480

Query: 481 RLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVVNC 540
           RLTVA GHKVEQKMSLTLFMK GL VNVH+RDLE +++S+  K  N          VV  
Sbjct: 481 RLTVAPGHKVEQKMSLTLFMKNGLLVNVHKRDLEVMMKSLVPKERND---------VVVL 537

Query: 541 NGGCNG 546
           NG CNG
Sbjct: 541 NGKCNG 537

BLAST of Lsi09G015720 vs. TAIR 10
Match: AT1G01600.1 (cytochrome P450, family 86, subfamily A, polypeptide 4 )

HSP 1 Score: 847.0 bits (2187), Expect = 8.3e-246
Identity = 410/548 (74.82%), Postives = 475/548 (86.68%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           M++  A+++V  VT Y LWF  ISR LKGP++WPLLGSLPGLIE  DR+H+WI++NLRAC
Sbjct: 1   MEISNAMLLVAIVTGYWLWFKRISRWLKGPRVWPLLGSLPGLIEQRDRMHEWITENLRAC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+A PF AKKQGLVTVTCDPKNLEH+LKTRFDNYPKGPTWQ+VFH+LLGQGIF
Sbjct: 61  GGTYQTCIFAVPFLAKKQGLVTVTCDPKNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTWLFQRKTAALEFTTRTLRQAM RWV+R IKLRFCPIL  A+ N+EP+DLQD++L
Sbjct: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRGIKLRFCPILATAQDNAEPVDLQDLIL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGLAFGKD  TCAPG PENGFA AFDRATEASLQRFI+P+ +WK KKWL LG+
Sbjct: 181 RLTFDNICGLAFGKDTRTCAPGLPENGFASAFDRATEASLQRFIIPKFMWKLKKWLGLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDST--PHDDLLSRFMKKKEGNYSNAFLRH 300
           EVSLSRSL  I+EYL++VI++RK EL++Q +  T   HDDLLSRFM KK  +YS+ FL+H
Sbjct: 241 EVSLSRSLGEIDEYLAAVINTRKQELMSQQESGTHQRHDDLLSRFMMKKTESYSDTFLQH 300

Query: 301 VALNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRG-NNIQSWLEEPLG 360
           VALNFILAGRDTSSVALSWFFWL+T++P +E+KI+REIC+VLIETRG +++ SW EEPLG
Sbjct: 301 VALNFILAGRDTSSVALSWFFWLITMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLG 360

Query: 361 FEEIDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMK 420
           F+EIDRL+YLKAA++ETLRLYPSVPEDSKHV  DDVLPDGTFVPAGSSVTYSIY+ GRMK
Sbjct: 361 FDEIDRLVYLKAAISETLRLYPSVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMK 420

Query: 421 STWGEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLL 480
           STWGEDCLEF PERW++    KFI +D Y+FVAFNAGPRICLGKDLAYLQMK+IAAA+LL
Sbjct: 421 STWGEDCLEFNPERWISPIDGKFINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLL 480

Query: 481 RHRLTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESIRGKYGNSEGKDVPLVAVV 540
           RHRLTV  GHKVEQKMSLTLFMK GL VN+++RDL+GI++S+  K   S+G         
Sbjct: 481 RHRLTVVPGHKVEQKMSLTLFMKNGLLVNLYKRDLQGIIKSLVVK--KSDGVS------- 537

Query: 541 NCNGGCNG 546
             NG CNG
Sbjct: 541 --NGQCNG 537

BLAST of Lsi09G015720 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 796.6 bits (2056), Expect = 1.3e-230
Identity = 378/519 (72.83%), Postives = 444/519 (85.55%), Query Frame = 0

Query: 1   MDVGFALVIVTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRAC 60
           MD   A +I+T +  Y++WF  + RS KGP++WPL+GSLP LI N  R+HD+I+DNLR C
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMC 60

Query: 61  GGTYQTCIWAFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIF 120
           GGTYQTCI+  PF AKKQG VTVTCDPKNLEHILKTRFDNYPKGP+WQ+VFH+LLG GIF
Sbjct: 61  GGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIF 120

Query: 121 NSDGDTWLFQRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLL 180
           NSDGDTW FQRKTAALEFTTRTLRQAMARWV RAIK R  PIL+ A   +EPIDLQDVLL
Sbjct: 121 NSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLL 180

Query: 181 RLTFDNICGLAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGM 240
           RLTFDNICGL FGKDP T +P FPENGFA+AFD ATEA+LQRFI+PE +WK +KWLRLG+
Sbjct: 181 RLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 240

Query: 241 EVSLSRSLVHIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGNYSNAFLRHVA 300
           E  +SRS+ H++ YLS +I++RKLELL Q +D + HDDLLSRFMKKKE +YS+ +L++VA
Sbjct: 241 EDDMSRSISHVDNYLSEIINTRKLELLGQQQDESRHDDLLSRFMKKKE-SYSDKYLKYVA 300

Query: 301 LNFILAGRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEE 360
           LNFILAGRDTSSVA+SWFFWLV+LNP +EEKI+ EICT+LI+TR  N+  W +EPL F+E
Sbjct: 301 LNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFDE 360

Query: 361 IDRLIYLKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTW 420
           ID+L+YLKAAL+ETLRLYPSVPEDSK VVA+DVLPDGTFVP+GS+VTYSIYS GRMK  W
Sbjct: 361 IDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIW 420

Query: 421 GEDCLEFRPERWLTADGKKFIPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHR 480
           GEDCLEF+PERWL  +  +    + YKFVAFNAGPRICLGKDLAYLQMKSI A++LLRHR
Sbjct: 421 GEDCLEFKPERWL--EESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHR 480

Query: 481 LTVAAGHKVEQKMSLTLFMKYGLKVNVHRRDLEGIVESI 520
           LTVA GH+VEQKMSLTLFMK+GLK++VH+RDL   VE +
Sbjct: 481 LTVAPGHRVEQKMSLTLFMKFGLKMDVHKRDLTLPVEKV 516

BLAST of Lsi09G015720 vs. TAIR 10
Match: AT5G58860.1 (cytochrome P450, family 86, subfamily A, polypeptide 1 )

HSP 1 Score: 668.7 bits (1724), Expect = 4.0e-192
Identity = 329/505 (65.15%), Postives = 391/505 (77.43%), Query Frame = 0

Query: 10  VTAVTAYLLWFTFISRSLKGPQMWPLLGSLPGLIENCDRLHDWISDNLRACGGTYQTCIW 69
           V A++ Y LWF F+SR L GP++ P +GSLP LI N  R+HDWI+DNLRA GGTYQTC  
Sbjct: 13  VAALSVYALWFYFLSRRLTGPKVLPFVGSLPYLIANRSRIHDWIADNLRATGGTYQTCTM 72

Query: 70  AFPFFAKKQGLVTVTCDPKNLEHILKTRFDNYPKGPTWQAVFHELLGQGIFNSDGDTWLF 129
             PF AK QG  TVTC PKN+EHILKTRFDNYPKGP W+A FH+LLGQGIFNSDGDTWL 
Sbjct: 73  VIPFVAKAQGFYTVTCHPKNVEHILKTRFDNYPKGPMWRAAFHDLLGQGIFNSDGDTWLM 132

Query: 130 QRKTAALEFTTRTLRQAMARWVSRAIKLRFCPILKQAELNSEPIDLQDVLLRLTFDNICG 189
           QRKTAALEFTTRTLRQAMARWV+  IK R   IL +A  N++P+DLQD+ LRLTFDNICG
Sbjct: 133 QRKTAALEFTTRTLRQAMARWVNGTIKNRLWLILDRAVQNNKPVDLQDLFLRLTFDNICG 192

Query: 190 LAFGKDPMTCAPGFPENGFAMAFDRATEASLQRFILPEVLWKFKKWLRLGMEVSLSRSLV 249
           L FGKDP T +   P+N F++AFD ATEA+L+R +    LW+ +K + +G E  L +SL 
Sbjct: 193 LTFGKDPETLSLDLPDNPFSVAFDTATEATLKRLLYTGFLWRIQKAMGIGSEDKLKKSLE 252

Query: 250 HIEEYLSSVIDSRKLELLNQPKDSTPHDDLLSRFMKKKEGN---YSNAFLRHVALNFILA 309
            +E Y++  ID+RK         ++P DDLLSRF+KK++ N        L+ +ALNF+LA
Sbjct: 253 VVETYMNDAIDARK---------NSPSDDLLSRFLKKRDVNGNVLPTDVLQRIALNFVLA 312

Query: 310 GRDTSSVALSWFFWLVTLNPLIEEKILREICTVLIETRGNNIQSWLEEPLGFEEIDRLIY 369
           GRDTSSVALSWFFWLV  N  +E KI+ E+  VL ETRGN+ + W EEPL F+E DRL+Y
Sbjct: 313 GRDTSSVALSWFFWLVMNNREVETKIVNELSMVLKETRGNDQEKWTEEPLEFDEADRLVY 372

Query: 370 LKAALTETLRLYPSVPEDSKHVVADDVLPDGTFVPAGSSVTYSIYSTGRMKSTWGEDCLE 429
           LKAAL ETLRLYPSVP+D K+VV DDVLPDGTFVP GS+VTYSIYS GRMK+ WGEDCLE
Sbjct: 373 LKAALAETLRLYPSVPQDFKYVVDDDVLPDGTFVPRGSTVTYSIYSIGRMKTIWGEDCLE 432

Query: 430 FRPERWLTADGKKF-IPYDSYKFVAFNAGPRICLGKDLAYLQMKSIAAALLLRHRLTVAA 489
           FRPERWLTADG++F  P D YKFVAFNAGPR CLGKDLAY QMKS+A+A+LLR+R+    
Sbjct: 433 FRPERWLTADGERFETPKDGYKFVAFNAGPRTCLGKDLAYNQMKSVASAVLLRYRVFPVP 492

Query: 490 GHKVEQKMSLTLFMKYGLKVNVHRR 511
           GH+VEQKMSLTLFMK GL+V +  R
Sbjct: 493 GHRVEQKMSLTLFMKNGLRVYLQPR 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O808235.1e-24878.31Cytochrome P450 86A8 OS=Arabidopsis thaliana OX=3702 GN=CYP86A8 PE=2 SV=1[more]
O230661.1e-24775.82Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Q9LMM11.2e-24474.82Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
B3RFJ62.3e-23273.77Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9CAD61.8e-22972.83Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BVJ60.0e+0096.36Cytochrome P450 86A8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3C7C50.0e+0096.36cytochrome P450 86A8-like OS=Cucumis melo OX=3656 GN=LOC103497711 PE=3 SV=1[more]
A0A6J1H6760.0e+0096.54cytochrome P450 86A8 OS=Cucurbita moschata OX=3662 GN=LOC111459971 PE=3 SV=1[more]
A0A6J1KW550.0e+0096.36cytochrome P450 86A8 OS=Cucurbita maxima OX=3661 GN=LOC111499241 PE=3 SV=1[more]
A0A0A0K9Z10.0e+0096.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004550 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038907189.10.0e+0097.81cytochrome P450 86A8-like [Benincasa hispida][more]
XP_008458223.10.0e+0096.36PREDICTED: cytochrome P450 86A8-like [Cucumis melo] >TYK03018.1 cytochrome P450 ... [more]
XP_023547593.10.0e+0096.72cytochrome P450 86A8 [Cucurbita pepo subsp. pepo][more]
XP_022958814.10.0e+0096.54cytochrome P450 86A8 [Cucurbita moschata][more]
XP_023006552.10.0e+0096.36cytochrome P450 86A8 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G45970.13.6e-24978.31cytochrome P450, family 86, subfamily A, polypeptide 8 [more]
AT4G00360.18.0e-24975.82cytochrome P450, family 86, subfamily A, polypeptide 2 [more]
AT1G01600.18.3e-24674.82cytochrome P450, family 86, subfamily A, polypeptide 4 [more]
AT1G63710.11.3e-23072.83cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
AT5G58860.14.0e-19265.15cytochrome P450, family 86, subfamily A, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 369..380
score: 43.62
coord: 449..458
score: 49.59
coord: 306..323
score: 34.14
coord: 458..469
score: 38.77
IPR001128Cytochrome P450PFAMPF00067p450coord: 28..497
e-value: 1.1E-60
score: 205.7
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 368..386
score: 27.97
coord: 315..341
score: 27.69
coord: 295..312
score: 29.22
coord: 458..481
score: 27.26
coord: 448..458
score: 46.92
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..513
e-value: 1.2E-95
score: 322.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 21..510
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 7..523
NoneNo IPR availablePANTHERPTHR24296:SF117CYTOCHROME P450 86A2coord: 7..523
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 451..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G015720.1Lsi09G015720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen