Lsi09G015380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G015380
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr09: 23511637 .. 23513395 (-)
RNA-Seq Expression	Lsi09G015380
Synteny	Lsi09G015380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCCCCAAGGACCCAAAATGGGAAGAATATTGCAAGCCATTGTCTGTTTAACATTCGTCTTTATGTTATCATCCCCGTTAAGTTATTCCTTACCCTTGTCAACTCAAGGAAGATGGATCGTTGATTCTAAAACAGGACGTCGAGTAAAGCTTGTATGTGTGAATTGGCCTTCCCATACCCAAAGCATGTTGGCAGAAGGCCTAAACCACAGGCCACTAAAAGAACTTGCTGATGAGGCAATCAAGTTGAAGTTCAATTGTGTGTGTCTCACATATGCAACTCACATGTTCACTCGCTAAGCTAATAGGACAATTGAAGAGAACTTTGAAGTTGTTGTTGATGTGCTTGGGGCAAGTGGTTTAATGGTGATTGCTGACAACCACATCAGCCAACCGAGATGGTGTTGTTCTCTTGACGACGGCAATGGATTCTTTGGAAACCGCAATTTTGACCCTCAAGAATGGTTACAAGGTTTGAGCTTAGTTGCTCGACGTTTTATCAACAAATCAACGATATGTAACTTTAAAAGATTTTTCATACACTTTTTTAAACTAACTCCAACCATTCGAGGACTACAAACTGTGTGGCAGTATTTCATTAATATTGAAACAAGATTCTATGACTTATAGGTGATAGCAATGAGCTTACGAAATGAGATACGAGGAACAATGGAAAATGCAAATGATTGGAACAAGTATGTAACTCAAGGAGTAACAACAATCCACAACATAAACCCAAATGTTTTAGTGATTGTTTCAGGCCTAAATTATGATAACGATCTTAGATGCCTAAAGGAGAAGCCCTTGACTGTAAACGCTTTAGACAATAAATTGGTTTTTGAGGTACACTTGTATTCTTTTAGTGGAGATGAGAGCAGGTACGTACAACAACCATTGAACAACATTTGTGCAAATGTCATAAACGGGTTTGTAGACCATGCAGGGTTTATAATGGAAGGACCAAATCCATTTCCTTTGTTTGTAAGTGAATATGGGTACGATCAAAGAGGGACCAACGATGCTGAAAATCGATACATGAGTTGCTTTACAGCTCATCTTGCTCTAAAAGATATGGATTGGGCACTATGGACTTGGCAGGGAAGTTATTATTATAGGGAAGGTGAAGCAGAGCCTACAGAAGTATTTGGAGTTCTCAACTCCAATTGGACACAGATTCAAAACCCTAATTTTACTAAGAAGTTTCAGCTATTGCAGACCATGTTGCAAGGTAATTGTATTAATCATTACACATGCACAAACTATAAATTTGTAAAGCAATTTGTAAGTTTAAAATAGAATTTAATTGTGTTGCATTTTTGTGGCATGTAGATCCGAGTTCCAATGCATCATCTTCTTATGTTATGTATCATCCACAAAGTGGCCAATGTGCCCAAGCTTCAAATGACAATACTGCAATATTTTTGAGTAATTGTTCCACCCCAAGTCAATGGAGTCATGGTGATGGTGGCACTCCAATCAAAATGACGACAAATGGTTTGTGTTTAAAGGCTAATGGTGAAGGTATTGGAGTATCCCTCTCAAGTGATTGCTTGGGTCAACAATGCGTTTGGAGAGCAATTTCTAACAGTAATCTTCATTTGGCCACTGTCACTCAAGATGGGAAGAGTCTTTGCTTGGAGGTTGAAAGCTCCACTTCTTCAAAAATTGTGACCAACTCTTGTATTTGTACCAATGGCGAGCCAAACTGCCTTCAAGACACCCAAAGTCAATGGTTTGAACTTGTTGCAACCAACACATTG

mRNA sequence

TCCCCCAAGGACCCAAAATGGGAAGAATATTGCAAGCCATTGTCTGTTTAACATTCGTCTTTATGTTATCATCCCCGTTAAGTTATTCCTTACCCTTGTCAACTCAAGGAAGATGGATCGTTGATTCTAAAACAGGACGTCGAGTAAAGCTTGTATGTGTGAATTGGCCTTCCCATACCCAAAGCATGTTGGCAGAAGGCCTAAACCACAGGCCACTAAAAGAACTTGCTGATGAGGCAATCAAGTTGAAGTTCAATTGTGTGTGTCTCACATATGCAACTCACATGACAATTGAAGAGAACTTTGAAGTTGTTGTTGATGTGCTTGGGGCAAGTGGTTTAATGGTGATTGCTGACAACCACATCAGCCAACCGAGATGGTGTTGTTCTCTTGACGACGGCAATGGATTCTTTGGAAACCGCAATTTTGACCCTCAAGAATGGTTACAAGGTTTGAGCTTAGTTGCTCGACGTTTTATCAACAAATCAACGATATGTAACTTTAAAAGATTTTTCATACACTTTTTTAAACTAACTCCAACCATTCGAGGACTACAAACTGTGATAGCAATGAGCTTACGAAATGAGATACGAGGAACAATGGAAAATGCAAATGATTGGAACAAGTATGTAACTCAAGGAGTAACAACAATCCACAACATAAACCCAAATGTTTTAGTGATTGTTTCAGGCCTAAATTATGATAACGATCTTAGATGCCTAAAGGAGAAGCCCTTGACTGTAAACGCTTTAGACAATAAATTGGTTTTTGAGGTACACTTGTATTCTTTTAGTGGAGATGAGAGCAGGTACGTACAACAACCATTGAACAACATTTGTGCAAATGTCATAAACGGGTTTGTAGACCATGCAGGGTTTATAATGGAAGGACCAAATCCATTTCCTTTGTTTGTAAGTGAATATGGGTACGATCAAAGAGGGACCAACGATGCTGAAAATCGATACATGAGTTGCTTTACAGCTCATCTTGCTCTAAAAGATATGGATTGGGCACTATGGACTTGGCAGGGAAGTTATTATTATAGGGAAGGTGAAGCAGAGCCTACAGAAGTATTTGGAGTTCTCAACTCCAATTGGACACAGATTCAAAACCCTAATTTTACTAAGAAGTTTCAGCTATTGCAGACCATGTTGCAAGATCCGAGTTCCAATGCATCATCTTCTTATGTTATGTATCATCCACAAAGTGGCCAATGTGCCCAAGCTTCAAATGACAATACTGCAATATTTTTGAGTAATTGTTCCACCCCAAGTCAATGGAGTCATGGTGATGGTGGCACTCCAATCAAAATGACGACAAATGGTTTGTGTTTAAAGGCTAATGGTGAAGGTATTGGAGTATCCCTCTCAAGTGATTGCTTGGGTCAACAATGCGTTTGGAGAGCAATTTCTAACAGTAATCTTCATTTGGCCACTGTCACTCAAGATGGGAAGAGTCTTTGCTTGGAGGTTGAAAGCTCCACTTCTTCAAAAATTGTGACCAACTCTTGTATTTGTACCAATGGCGAGCCAAACTGCCTTCAAGACACCCAAAGTCAATGGTTTGAACTTGTTGCAACCAACACATTG

Coding sequence (CDS)

ATGGGAAGAATATTGCAAGCCATTGTCTGTTTAACATTCGTCTTTATGTTATCATCCCCGTTAAGTTATTCCTTACCCTTGTCAACTCAAGGAAGATGGATCGTTGATTCTAAAACAGGACGTCGAGTAAAGCTTGTATGTGTGAATTGGCCTTCCCATACCCAAAGCATGTTGGCAGAAGGCCTAAACCACAGGCCACTAAAAGAACTTGCTGATGAGGCAATCAAGTTGAAGTTCAATTGTGTGTGTCTCACATATGCAACTCACATGACAATTGAAGAGAACTTTGAAGTTGTTGTTGATGTGCTTGGGGCAAGTGGTTTAATGGTGATTGCTGACAACCACATCAGCCAACCGAGATGGTGTTGTTCTCTTGACGACGGCAATGGATTCTTTGGAAACCGCAATTTTGACCCTCAAGAATGGTTACAAGGTTTGAGCTTAGTTGCTCGACGTTTTATCAACAAATCAACGATATGTAACTTTAAAAGATTTTTCATACACTTTTTTAAACTAACTCCAACCATTCGAGGACTACAAACTGTGATAGCAATGAGCTTACGAAATGAGATACGAGGAACAATGGAAAATGCAAATGATTGGAACAAGTATGTAACTCAAGGAGTAACAACAATCCACAACATAAACCCAAATGTTTTAGTGATTGTTTCAGGCCTAAATTATGATAACGATCTTAGATGCCTAAAGGAGAAGCCCTTGACTGTAAACGCTTTAGACAATAAATTGGTTTTTGAGGTACACTTGTATTCTTTTAGTGGAGATGAGAGCAGGTACGTACAACAACCATTGAACAACATTTGTGCAAATGTCATAAACGGGTTTGTAGACCATGCAGGGTTTATAATGGAAGGACCAAATCCATTTCCTTTGTTTGTAAGTGAATATGGGTACGATCAAAGAGGGACCAACGATGCTGAAAATCGATACATGAGTTGCTTTACAGCTCATCTTGCTCTAAAAGATATGGATTGGGCACTATGGACTTGGCAGGGAAGTTATTATTATAGGGAAGGTGAAGCAGAGCCTACAGAAGTATTTGGAGTTCTCAACTCCAATTGGACACAGATTCAAAACCCTAATTTTACTAAGAAGTTTCAGCTATTGCAGACCATGTTGCAAGATCCGAGTTCCAATGCATCATCTTCTTATGTTATGTATCATCCACAAAGTGGCCAATGTGCCCAAGCTTCAAATGACAATACTGCAATATTTTTGAGTAATTGTTCCACCCCAAGTCAATGGAGTCATGGTGATGGTGGCACTCCAATCAAAATGACGACAAATGGTTTGTGTTTAAAGGCTAATGGTGAAGGTATTGGAGTATCCCTCTCAAGTGATTGCTTGGGTCAACAATGCGTTTGGAGAGCAATTTCTAACAGTAATCTTCATTTGGCCACTGTCACTCAAGATGGGAAGAGTCTTTGCTTGGAGGTTGAAAGCTCCACTTCTTCAAAAATTGTGACCAACTCTTGTATTTGTACCAATGGCGAGCCAAACTGCCTTCAAGACACCCAAAGTCAATGGTTTGAACTTGTTGCAACCAACACATTG

Protein sequence

MGRILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGLNHRPLKELADEAIKLKFNCVCLTYATHMTIEENFEVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDESRYVQQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLALKDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNASSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGIGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPNCLQDTQSQWFELVATNTL

Homology

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BLAST of Lsi09G015380 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 9.1e-98
Identity = 212/571 (37.13%), Postives = 308/571 (53.94%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R+L A++ L    ++++P S+SLPL T+GRWIVD  TG RVKL CVNW  H +  L EGL
Sbjct: 12  RLLTALLLL----LVAAP-SHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGL 71

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHMT-------------------------IEEN-- 122
           N  P+  +A     L FNCV LTY+ HM                          IE N  
Sbjct: 72  NRLPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNP 131

Query: 123 ----------FEVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 182
                     +  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+R F+P  W++G
Sbjct: 132 ELLDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEG 191

Query: 183 LSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYV 242
           L L+A  F N                 TP       V+AMSLRNE+RG       W++++
Sbjct: 192 LGLMATYFNN-----------------TP------NVVAMSLRNELRGNRSTPISWSRHM 251

Query: 243 TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDESRY 302
             G  T+H  NP VLVI+SGL +D DL  L   P+T+     K+V+E H YSF       
Sbjct: 252 QWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL-PFKEKIVYEGHWYSFGVPWRTG 311

Query: 303 VQQPLNNICANVINGFVDHAGFIMEGPN--PFPLFVSEYGYDQRGTNDAENRYMSCFTAH 362
           +    N++C N    F  + GF+    N    PLF+SE+G DQR  ND +NRY++C  A+
Sbjct: 312 LP---NDVCKNETGRFKSNVGFVTSSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAY 371

Query: 363 LALKDMDWALWTWQGSYYYREGEAEPT----EVFGVLNSNWTQIQNPNFTKKFQLLQTML 422
           LA +D+DWALWT  GSYYYR  + +P     E +G  N +W++I+NP+F  + + +Q  +
Sbjct: 372 LAEEDLDWALWTMGGSYYYR-SDKQPVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPI 431

Query: 423 QDPSSNASSSY-VMYHPQSGQCAQASNDNTAIFLSNC-STPSQWSH-GDGGTPIKMTTNG 482
           QDP       Y ++YHP SG C ++   NT + L +C S  S+W++      PI +  + 
Sbjct: 432 QDPYLAPGPYYQIIYHPASGLCVESGIGNT-VHLGSCQSVRSRWNYDASVKGPIGLMGSS 491

Query: 483 LCLKANGEGIGVSLSSDCLG-QQCVWRAISNSNLHLAT--VTQDGKSLCLEVESSTSSKI 523
            C+   G G+   ++ +C      +W  +S++ L L T  + +DGK   + ++ S S  I
Sbjct: 492 SCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLI 547

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BLAST of Lsi09G015380 vs. ExPASy TrEMBL
Match: A0A6J1H6A6 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459997 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.4e-245
Identity = 422/558 (75.63%), Postives = 458/558 (82.08%), Query Frame = 0


Query: 1   MGRILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAE 60
           M R LQAIVCL  VF+ SS  + SLPLST+GRWI+DSKTGRRVKLVCVNWPSHTQSML E
Sbjct: 1   MRRTLQAIVCLALVFIFSSLSTCSLPLSTRGRWIIDSKTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVCLTYATHM-------TIEEN------------------ 120
           GLNHRPLKELADEAIKLKFNCV LTYATHM       TIEEN                  
Sbjct: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKPAKAGLVQYNP 120

Query: 121 ----------FEVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
                     FE VVDVLG SGLMVIADNHISQPRWCCSLDDGNGFFG+R FDPQEWLQG
Sbjct: 121 FVLNKTIAEAFEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYV 240
           L LVA RFINK                         +IAMSLRNEIRGT ENANDWN Y+
Sbjct: 181 LQLVAWRFINK-----------------------PAMIAMSLRNEIRGTRENANDWNNYI 240

Query: 241 TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESR 300
           TQGVT +HN NP++LVIVSGLN+DNDLRCLKEKPL V  LDNKLVFEVHLYS SGD ES+
Sbjct: 241 TQGVTIVHNTNPDILVIVSGLNFDNDLRCLKEKPLNVTNLDNKLVFEVHLYSLSGDPESK 300

Query: 301 YVQQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHL 360
           +VQQPLNNICAN+INGFVDHAGF+M+GPNPFPLFVSEYGYDQR +NDAENR+MSCFT+HL
Sbjct: 301 FVQQPLNNICANIINGFVDHAGFVMDGPNPFPLFVSEYGYDQRESNDAENRFMSCFTSHL 360

Query: 361 ALKDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSS 420
           A KD+DWALWTWQGSYYYREG+AEPTEVFGVLNSNWT+IQNPNF+KKF LLQ+MLQDP+S
Sbjct: 361 AQKDLDWALWTWQGSYYYREGQAEPTEVFGVLNSNWTKIQNPNFSKKFHLLQSMLQDPNS 420

Query: 421 NASSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGE 480
           NAS SY++YHPQSGQCAQ SN++T IFLS+CST S+WSHGDGG  IKM T GLCLKANGE
Sbjct: 421 NASFSYILYHPQSGQCAQTSNNDTQIFLSDCSTSSRWSHGDGGNSIKMATTGLCLKANGE 480

Query: 481 GIGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGE 523
           G+GVSLSSDCL QQ VWR ISN+NLHLATVTQDGK LCL+VESS+SSKIVTN CICTNGE
Sbjct: 481 GLGVSLSSDCLSQQSVWRTISNTNLHLATVTQDGKYLCLQVESSSSSKIVTNPCICTNGE 535

[Close]

BLAST of Lsi09G015380 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.2e-233
Identity = 399/558 (71.51%), Postives = 448/558 (80.29%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R    I+ +  VF+ S   + SLPLST+GRWI+DS+TGRRVKLVC+NWPSHTQSML EGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF------------------- 122
           NHRPLK+LADEAIKL+FNCV LTYATHM       TIEENF                   
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ---------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLS 182
                    E VVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQ 242
           LVA+RF NKS                       TV+ MSLRNEIRG MENANDWNKY+TQ
Sbjct: 182 LVAQRFRNKS-----------------------TVVGMSLRNEIRGFMENANDWNKYITQ 241

Query: 243 GVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESRYV 302
           GVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V  LDNKLVFEVHLYSFSGD ES++V
Sbjct: 242 GVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV 301

Query: 303 QQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLAL 362
           +QPLN+ICAN++NGF+DHAGF+M+G NPFPLFVSE+GYDQR  NDAENR+MSCFTAHLAL
Sbjct: 302 KQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLAL 361

Query: 363 KDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNA 422
           +D+DWALW WQGSYY+REG+AEP E FGVL+SNWTQ++NPNF KKFQLLQTMLQDP+SN 
Sbjct: 362 RDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNE 421

Query: 423 SSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGI 482
           S SYV+YHPQS QC Q SNDN  IFL++CSTP++WSH +  TPIKM++ GLCLKA+GEG+
Sbjct: 422 SFSYVIYHPQSSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGL 481

Query: 483 GVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPN 525
             SLS+DCL QQ VW AISNS LHLATVTQ GKSLCL+++SS SSK+VTNSCICTNG+PN
Sbjct: 482 EASLSTDCLSQQSVWSAISNSKLHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPN 536

[Close]

BLAST of Lsi09G015380 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 1.0e-232
Identity = 398/558 (71.33%), Postives = 447/558 (80.11%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R    I+ L  VF+ S   + SLPLST+GRWI+D +TGRRVKLVC+NWPSHTQSML EGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF------------------- 122
           NHRPLK+LADEAIKL+FNCV LTYATHM       TIEENF                   
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ---------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLS 182
                    E VVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQ 242
           LVA+RF NKS                       TV+ MSLRNEIRG MENANDWNKY+TQ
Sbjct: 182 LVAQRFRNKS-----------------------TVVGMSLRNEIRGFMENANDWNKYITQ 241

Query: 243 GVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESRYV 302
           GVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V  LDNKLVFEVHLYSFSGD ES++V
Sbjct: 242 GVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV 301

Query: 303 QQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLAL 362
           +QPLNNICAN++NGF+DHAGF+M+G NPFPLFVSE+GYDQR  NDAENR+MSCFTAHLAL
Sbjct: 302 KQPLNNICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLAL 361

Query: 363 KDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNA 422
           +D+DWALW WQGSYY+REG+AEP E FGVL+SNWTQ++NPNF KKFQLLQTMLQDP+SNA
Sbjct: 362 RDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNA 421

Query: 423 SSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGI 482
           S SYV+YHPQ+ QC Q SNDN  IFL++CSTP++WSH +  TPIKM++ GLCLKA+GEG+
Sbjct: 422 SFSYVIYHPQTSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGL 481

Query: 483 GVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPN 525
             SLS+DCL QQ VW AISNS LHLATV Q G SLCL+++SS SSK+VTNSCICTNG+PN
Sbjct: 482 EASLSTDCLIQQSVWSAISNSKLHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPN 536

[Close]

BLAST of Lsi09G015380 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.7e-232
Identity = 398/559 (71.20%), Postives = 448/559 (80.14%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R    I+ L  VF+ S   + SLPLST+GRWI+DS+TGRRVKLVC+NWPSHTQSML EGL
Sbjct: 5   RTFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 64

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF------------------- 122
           NHRPLK+LADEAIKL+FNCV LTYATHM       TIEENF                   
Sbjct: 65  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFV 124

Query: 123 ---------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLS 182
                    E VVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGLS
Sbjct: 125 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 184

Query: 183 LVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQ 242
           LVA+RF NKS                       TV+ MSLRNEIRG MENANDWNKY+TQ
Sbjct: 185 LVAQRFRNKS-----------------------TVVGMSLRNEIRGFMENANDWNKYITQ 244

Query: 243 GVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESRYV 302
           GVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V  LDNKLVFEVHLYSFSGD ES++V
Sbjct: 245 GVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV 304

Query: 303 QQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLAL 362
           +QPLN+ICAN++NGF+DHAGF+M+G NPFPLFVSE+GYDQR  NDAENR+MSCFTAHLAL
Sbjct: 305 KQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLAL 364

Query: 363 KDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNA 422
           +D+DWALW WQGSYY+REG+AEP E FGVL+SNWTQ++NPNF KKFQLLQTMLQDP+SNA
Sbjct: 365 RDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNA 424

Query: 423 SSSYVMYHPQSGQCAQASNDNTAIFLSNC-STPSQWSHGDGGTPIKMTTNGLCLKANGEG 482
           S SYV+YHPQS QC Q SNDN  IFL++C STP++WSH +  TPIKM++ GLCLKA+GEG
Sbjct: 425 SFSYVIYHPQSSQCIQVSNDNKEIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEG 484

Query: 483 IGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEP 525
           +  SLS+DCL QQ VW AISNS LHLAT+TQ GKSLCL+++SS  SK+VTNSCICTNG+P
Sbjct: 485 LEASLSTDCLSQQSVWSAISNSKLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDP 540

[Close]

BLAST of Lsi09G015380 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 6.5e-232
Identity = 403/560 (71.96%), Postives = 442/560 (78.93%), Query Frame = 0


Query: 1   MGRILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAE 60
           MGRI Q +  L  +F+  SP  YSLPLST+ +WI+DS TGRRVKLVCVNWPSHTQSML E
Sbjct: 1   MGRIFQVVFFLA-LFVFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF----------------- 120
           GLNHRPLKELADEAIKL+FNCV LTYAT M       T+EENF                 
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNP 120

Query: 121 -----------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
                      E VVDVLG SGLMVIADNHISQPRWCCSLDDGNGFFG+R FDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYV 240
           LSLVA+RF  KS                       TV+ MSLRNEIRGT ENANDWN YV
Sbjct: 181 LSLVAQRFSKKS-----------------------TVVGMSLRNEIRGTNENANDWNNYV 240

Query: 241 TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESR 300
           TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPL V+ LDNKLVFEVHLYSFSGD ES+
Sbjct: 241 TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESK 300

Query: 301 YVQQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHL 360
           ++ QPLNNICAN+INGFVDHA F+ EG NPFPLFVSEYGYDQR  NDAENR+MSCFTAHL
Sbjct: 301 FINQPLNNICANIINGFVDHAEFVTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHL 360

Query: 361 ALKDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSS 420
           A KD+DWALWTWQGSYYYREG+AEP E FGVL+SNWTQI+NPNF +KFQLLQTMLQDP+S
Sbjct: 361 AQKDLDWALWTWQGSYYYREGQAEPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNS 420

Query: 421 NASSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGE 480
           NAS SYV+YHPQSGQC Q SNDN  IF+ NCS  S+W+H +  TPI+M++ GLCLK  GE
Sbjct: 421 NASFSYVIYHPQSGQCIQVSNDNKDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGE 480

Query: 481 GIGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGE 525
           G+  SLS+DCLG Q  W AISN+ LHLAT++QDGKSLCL+VESS SSKIVTNSCICTNG 
Sbjct: 481 GLTPSLSTDCLGPQSSWSAISNTKLHLATISQDGKSLCLQVESSNSSKIVTNSCICTNGA 536

[Close]

BLAST of Lsi09G015380 vs. NCBI nr
Match: XP_022958844.1 (uncharacterized protein LOC111459997 [Cucurbita moschata])

HSP 1 Score: 858.6 bits (2217), Expect = 2.8e-245
Identity = 422/558 (75.63%), Postives = 458/558 (82.08%), Query Frame = 0


Query: 1   MGRILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAE 60
           M R LQAIVCL  VF+ SS  + SLPLST+GRWI+DSKTGRRVKLVCVNWPSHTQSML E
Sbjct: 1   MRRTLQAIVCLALVFIFSSLSTCSLPLSTRGRWIIDSKTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVCLTYATHM-------TIEEN------------------ 120
           GLNHRPLKELADEAIKLKFNCV LTYATHM       TIEEN                  
Sbjct: 61  GLNHRPLKELADEAIKLKFNCVRLTYATHMFTRYANRTIEENFDLLDLKPAKAGLVQYNP 120

Query: 121 ----------FEVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
                     FE VVDVLG SGLMVIADNHISQPRWCCSLDDGNGFFG+R FDPQEWLQG
Sbjct: 121 FVLNKTIAEAFEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 181 LSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYV 240
           L LVA RFINK                         +IAMSLRNEIRGT ENANDWN Y+
Sbjct: 181 LQLVAWRFINK-----------------------PAMIAMSLRNEIRGTRENANDWNNYI 240

Query: 241 TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESR 300
           TQGVT +HN NP++LVIVSGLN+DNDLRCLKEKPL V  LDNKLVFEVHLYS SGD ES+
Sbjct: 241 TQGVTIVHNTNPDILVIVSGLNFDNDLRCLKEKPLNVTNLDNKLVFEVHLYSLSGDPESK 300

Query: 301 YVQQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHL 360
           +VQQPLNNICAN+INGFVDHAGF+M+GPNPFPLFVSEYGYDQR +NDAENR+MSCFT+HL
Sbjct: 301 FVQQPLNNICANIINGFVDHAGFVMDGPNPFPLFVSEYGYDQRESNDAENRFMSCFTSHL 360

Query: 361 ALKDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSS 420
           A KD+DWALWTWQGSYYYREG+AEPTEVFGVLNSNWT+IQNPNF+KKF LLQ+MLQDP+S
Sbjct: 361 AQKDLDWALWTWQGSYYYREGQAEPTEVFGVLNSNWTKIQNPNFSKKFHLLQSMLQDPNS 420

Query: 421 NASSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGE 480
           NAS SY++YHPQSGQCAQ SN++T IFLS+CST S+WSHGDGG  IKM T GLCLKANGE
Sbjct: 421 NASFSYILYHPQSGQCAQTSNNDTQIFLSDCSTSSRWSHGDGGNSIKMATTGLCLKANGE 480

Query: 481 GIGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGE 523
           G+GVSLSSDCL QQ VWR ISN+NLHLATVTQDGK LCL+VESS+SSKIVTN CICTNGE
Sbjct: 481 GLGVSLSSDCLSQQSVWRTISNTNLHLATVTQDGKYLCLQVESSSSSKIVTNPCICTNGE 535

[Close]

BLAST of Lsi09G015380 vs. NCBI nr
Match: XP_038900453.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 845.5 bits (2183), Expect = 2.5e-241
Identity = 416/560 (74.29%), Postives = 450/560 (80.36%), Query Frame = 0


Query: 1   MGRILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAE 60
           MGR LQ IV L  VF      +YSLPLST+GRWIVDSKTG RVK VC NWPSHTQSMLAE
Sbjct: 1   MGRNLQTIVFLVLVFEFLFFSAYSLPLSTKGRWIVDSKTGHRVKFVCANWPSHTQSMLAE 60

Query: 61  GLNHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF----------------- 120
           GLNHRPLKELADEAIKL+FNCV LTYATHM       TIEENF                 
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQHNP 120

Query: 121 -----------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180
                      E VVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG
Sbjct: 121 FLLNKTIVEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQG 180

Query: 181 LSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYV 240
           L LVA+RF+NKS                       TV+AMSLRNEIRGTMENANDWN YV
Sbjct: 181 LRLVAKRFVNKS-----------------------TVVAMSLRNEIRGTMENANDWNNYV 240

Query: 241 TQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESR 300
           TQGVTTIHNINP VLVIVSGLNYDNDLRCLKEKPL VN LDNKLVFEVHLYSFSGD ES+
Sbjct: 241 TQGVTTIHNINPEVLVIVSGLNYDNDLRCLKEKPLNVNTLDNKLVFEVHLYSFSGDSESK 300

Query: 301 YVQQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHL 360
           +V QPLN+ICA ++NGFVDHAGF++EG NPFPLFVSEYGYDQR  N+AENR+MSCFTAHL
Sbjct: 301 FVTQPLNDICATIMNGFVDHAGFVIEGSNPFPLFVSEYGYDQREVNEAENRFMSCFTAHL 360

Query: 361 ALKDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSS 420
           A KD+DWALWTWQGSYYYREG+AEP E FGVL+SNWTQI+NPNF KKFQLLQTMLQDP+S
Sbjct: 361 AQKDLDWALWTWQGSYYYREGQAEPAETFGVLDSNWTQIKNPNFPKKFQLLQTMLQDPNS 420

Query: 421 NASSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGE 480
           NAS+SYVMYHPQSGQC QA +DN  +FL+NCSTPS+WSH   G+PI++ + GLCLKANGE
Sbjct: 421 NASTSYVMYHPQSGQCVQALDDNKGMFLNNCSTPSRWSHNGDGSPIRLRSTGLCLKANGE 480

Query: 481 GIGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGE 525
           G+G SLSSDCL QQ  WRAISNS LHLAT  QDGKSLCL++E+S SSKIV NSCICTNG+
Sbjct: 481 GLGASLSSDCLSQQSAWRAISNSKLHLATFAQDGKSLCLQLENSNSSKIVVNSCICTNGD 537

[Close]

BLAST of Lsi09G015380 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 818.9 bits (2114), Expect = 2.5e-233
Identity = 399/558 (71.51%), Postives = 448/558 (80.29%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R    I+ +  VF+ S   + SLPLST+GRWI+DS+TGRRVKLVC+NWPSHTQSML EGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF------------------- 122
           NHRPLK+LADEAIKL+FNCV LTYATHM       TIEENF                   
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ---------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLS 182
                    E VVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQ 242
           LVA+RF NKS                       TV+ MSLRNEIRG MENANDWNKY+TQ
Sbjct: 182 LVAQRFRNKS-----------------------TVVGMSLRNEIRGFMENANDWNKYITQ 241

Query: 243 GVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESRYV 302
           GVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V  LDNKLVFEVHLYSFSGD ES++V
Sbjct: 242 GVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV 301

Query: 303 QQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLAL 362
           +QPLN+ICAN++NGF+DHAGF+M+G NPFPLFVSE+GYDQR  NDAENR+MSCFTAHLAL
Sbjct: 302 KQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLAL 361

Query: 363 KDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNA 422
           +D+DWALW WQGSYY+REG+AEP E FGVL+SNWTQ++NPNF KKFQLLQTMLQDP+SN 
Sbjct: 362 RDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNE 421

Query: 423 SSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGI 482
           S SYV+YHPQS QC Q SNDN  IFL++CSTP++WSH +  TPIKM++ GLCLKA+GEG+
Sbjct: 422 SFSYVIYHPQSSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGL 481

Query: 483 GVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPN 525
             SLS+DCL QQ VW AISNS LHLATVTQ GKSLCL+++SS SSK+VTNSCICTNG+PN
Sbjct: 482 EASLSTDCLSQQSVWSAISNSKLHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPN 536

[Close]

BLAST of Lsi09G015380 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 815.8 bits (2106), Expect = 2.1e-232
Identity = 398/558 (71.33%), Postives = 447/558 (80.11%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R    I+ L  VF+ S   + SLPLST+GRWI+D +TGRRVKLVC+NWPSHTQSML EGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF------------------- 122
           NHRPLK+LADEAIKL+FNCV LTYATHM       TIEENF                   
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 123 ---------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLS 182
                    E VVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 183 LVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQ 242
           LVA+RF NKS                       TV+ MSLRNEIRG MENANDWNKY+TQ
Sbjct: 182 LVAQRFRNKS-----------------------TVVGMSLRNEIRGFMENANDWNKYITQ 241

Query: 243 GVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESRYV 302
           GVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V  LDNKLVFEVHLYSFSGD ES++V
Sbjct: 242 GVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV 301

Query: 303 QQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLAL 362
           +QPLNNICAN++NGF+DHAGF+M+G NPFPLFVSE+GYDQR  NDAENR+MSCFTAHLAL
Sbjct: 302 KQPLNNICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLAL 361

Query: 363 KDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNA 422
           +D+DWALW WQGSYY+REG+AEP E FGVL+SNWTQ++NPNF KKFQLLQTMLQDP+SNA
Sbjct: 362 RDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNA 421

Query: 423 SSSYVMYHPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGI 482
           S SYV+YHPQ+ QC Q SNDN  IFL++CSTP++WSH +  TPIKM++ GLCLKA+GEG+
Sbjct: 422 SFSYVIYHPQTSQCIQVSNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGL 481

Query: 483 GVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPN 525
             SLS+DCL QQ VW AISNS LHLATV Q G SLCL+++SS SSK+VTNSCICTNG+PN
Sbjct: 482 EASLSTDCLIQQSVWSAISNSKLHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPN 536

[Close]

BLAST of Lsi09G015380 vs. NCBI nr
Match: KAA0042709.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 815.1 bits (2104), Expect = 3.6e-232
Identity = 398/559 (71.20%), Postives = 448/559 (80.14%), Query Frame = 0


Query: 3   RILQAIVCLTFVFMLSSPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGL 62
           R    I+ L  VF+ S   + SLPLST+GRWI+DS+TGRRVKLVC+NWPSHTQSML EGL
Sbjct: 5   RTFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 64

Query: 63  NHRPLKELADEAIKLKFNCVCLTYATHM-------TIEENF------------------- 122
           NHRPLK+LADEAIKL+FNCV LTYATHM       TIEENF                   
Sbjct: 65  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALYNPFV 124

Query: 123 ---------EVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLS 182
                    E VVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQEWLQGLS
Sbjct: 125 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 184

Query: 183 LVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQ 242
           LVA+RF NKS                       TV+ MSLRNEIRG MENANDWNKY+TQ
Sbjct: 185 LVAQRFRNKS-----------------------TVVGMSLRNEIRGFMENANDWNKYITQ 244

Query: 243 GVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGD-ESRYV 302
           GVTTIHNIN  VLVIVSGLNYDNDLRCLKEKPL V  LDNKLVFEVHLYSFSGD ES++V
Sbjct: 245 GVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFV 304

Query: 303 QQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLAL 362
           +QPLN+ICAN++NGF+DHAGF+M+G NPFPLFVSE+GYDQR  NDAENR+MSCFTAHLAL
Sbjct: 305 KQPLNDICANIMNGFIDHAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLAL 364

Query: 363 KDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNA 422
           +D+DWALW WQGSYY+REG+AEP E FGVL+SNWTQ++NPNF KKFQLLQTMLQDP+SNA
Sbjct: 365 RDLDWALWAWQGSYYFREGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNA 424

Query: 423 SSSYVMYHPQSGQCAQASNDNTAIFLSNC-STPSQWSHGDGGTPIKMTTNGLCLKANGEG 482
           S SYV+YHPQS QC Q SNDN  IFL++C STP++WSH +  TPIKM++ GLCLKA+GEG
Sbjct: 425 SFSYVIYHPQSSQCIQVSNDNKEIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEG 484

Query: 483 IGVSLSSDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEP 525
           +  SLS+DCL QQ VW AISNS LHLAT+TQ GKSLCL+++SS  SK+VTNSCICTNG+P
Sbjct: 485 LEASLSTDCLSQQSVWSAISNSKLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDP 540

[Close]

BLAST of Lsi09G015380 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 374.0 bits (959), Expect = 2.0e-103
Identity = 202/551 (36.66%), Postives = 308/551 (55.90%), Query Frame = 0


Query: 10  CLTFVFMLSSPL-SYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGLNHRPLK 69
           C  F F+  + + + S PLST  RWIVD + G RVKLVC NWPSH Q ++AEGL+ +P+ 
Sbjct: 17  CFFFSFIAQNTVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVD 76

Query: 70  ELADEAIKLKFNCVCLTY----------ATHMTIEENFE--------------------- 129
            +A + +++ FNCV LT+          A ++T+ ++F+                     
Sbjct: 77  AVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDL 136

Query: 130 -------VVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLSLVA 189
                   VV  LG + +MVI DNH+++P WCC+ DDGNGFFG++ FDP  W+  L    
Sbjct: 137 PLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALK--- 196

Query: 190 RRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQGVT 249
                               K+  T  G+  V+ MSLRNE+RG  +N NDW KY+ QG  
Sbjct: 197 --------------------KMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAE 256

Query: 250 TIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDESRYVQQPL 309
            +H+ N  VLVI+SGL++D DL  ++ +P+ + +   KLVFE+H YSFS   S     P 
Sbjct: 257 AVHSANNKVLVILSGLSFDADLSFVRSRPVKL-SFTGKLVFELHWYSFSDGNSWAANNP- 316

Query: 310 NNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLALKDMD 369
           N+IC  V+N   +  G+++     FPLF+SE+G D+RG N  +NRY  C T   A  D+D
Sbjct: 317 NDICGRVLNRIGNGGGYLLN--QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVD 376

Query: 370 WALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNASSSY 429
           W+LW   GSYY R+G+    E +GVL+S+W  ++N +F +K   LQ+ LQ P     +  
Sbjct: 377 WSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYN 436

Query: 430 VMYHPQSGQC-AQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGIGVS 489
           +++HP +G C  ++ +D   + L  C++   WS+      IK     LCL++NG    V+
Sbjct: 437 LVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKKALRIK--DQQLCLQSNGPKNPVT 496

Query: 490 LS-SDCLGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPNCL 520
           ++ + C      W+ IS S +HLA+ T +  SLCL+V+  T++ +V N+C C + + +C 
Sbjct: 497 MTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDVD--TANNVVANACKCLSKDKSC- 533

[Close]

BLAST of Lsi09G015380 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.9e-98
Identity = 204/549 (37.16%), Postives = 293/549 (53.37%), Query Frame = 0


Query: 14  VFMLSSPL-SYSLPLSTQGRWIV-DSKTGRRVKLVCVNWPSHTQSMLAEGLNHRPLKELA 73
           VF+L   + +++ P ST  RWIV D   GRRVKL CVNWPSH ++ +AEGL+ +PL  +A
Sbjct: 9   VFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIA 68

Query: 74  DEAIKLKFNCVCLTYATHMTIEEN------------------------------------ 133
           ++ + + FNCV LT+  ++  +E+                                    
Sbjct: 69  EKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLI 128

Query: 134 --FEVVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLSLVARRF 193
             F+ VV  L    +MVI DNHISQP WCCS +DGNGFFG+++ +PQ W++GL  +A  F
Sbjct: 129 KAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMF 188

Query: 194 INKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQGVTTIH 253
            N S+                       V+ MSLRNE+RG  +N  DW KY+ +G   +H
Sbjct: 189 ANVSS----------------------NVVGMSLRNELRGPKQNIKDWYKYMREGAEAVH 248

Query: 254 NINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDESRYVQQPLNNI 313
           ++NPNVLVIVSGLNY  DL  L+E+P  V +   K+VFE+H Y F    + +    LN I
Sbjct: 249 SVNPNVLVIVSGLNYATDLSFLRERPFEV-SFRRKVVFEIHWYGF---WNTWEGDNLNKI 308

Query: 314 CANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLALKDMDWAL 373
           C       +  +GF++E     PLFVSE+G DQRG N  +N+++SCF A  A +D+DW+L
Sbjct: 309 CGKETEKMMKMSGFLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSL 368

Query: 374 WTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNASSSYVMY 433
           WT  GSYY RE      E +GVL+ NW+ I+N    +    +QT             +M+
Sbjct: 369 WTLAGSYYIREKSIGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIG-LMETQPKKIMF 428

Query: 434 HPQSGQCAQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGIGVSLSSD 493
           HP +G C         + L +C+    W               LCLKA  +G  V L   
Sbjct: 429 HPSTGLCI-VRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLF 488

Query: 494 CLGQQC-VWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPNCLQDTQ 522
                C  W+  S+S + L+++T++G S+CL+V++  ++ IVTNSC C  G  +C  D +
Sbjct: 489 FSESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTE-NNNIVTNSCKCLRGNSSC--DPR 524

[Close]

BLAST of Lsi09G015380 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 346.7 bits (888), Expect = 3.3e-95
Identity = 191/535 (35.70%), Postives = 291/535 (54.39%), Query Frame = 0


Query: 26  PLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGLNHRPLKELADEAIKLKFNCVCLT 85
           PLST  RWI+D K G+RVKL CVNWPSH Q ++AEGL+ + + +LA + + + FNCV  T
Sbjct: 4   PLSTNSRWIIDEK-GQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  Y----------ATHMTIEENFE----------------------------VVVDVLGASG 145
           +          A ++T+ ++F+                             VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLSLVARRFINKSTICNFKRFFI 205
           +MVI DNH+++P WCC  +DGNGFFG+  FDP  W+ GL+                    
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLT-------------------- 183

Query: 206 HFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQGVTTIHNINPNVLVIVSGLN 265
              K+  T +G   V+ MSLRNE+RG  +N +DW KY+ QG   +H  NPNVLVI+SGL+
Sbjct: 184 ---KIAMTFKGATNVVGMSLRNELRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLS 243

Query: 266 YDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDESRYVQQPLNNICANVINGFVDHAGF 325
           YD DL  ++ + + +     KLVFE+H YSF+   +   + P N  C  ++    +  GF
Sbjct: 244 YDTDLSFVRSRHVNL-TFTRKLVFELHRYSFTNTNTWSSKNP-NEACGEILKSIENGGGF 303

Query: 326 IMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLALKDMDWALWTWQGSYYYREGEA 385
            +     FP+F+SE+G D RG N  +NRY+ C     A  D+DW++WT QGSYY REG  
Sbjct: 304 NL---RDFPVFLSEFGIDLRGKNVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVV 363

Query: 386 EPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNASSSYVMYHPQSGQC-AQASND 445
             +E +G+L+S+W ++++ +F ++  L+ + LQ P S +    +++HP +G C  Q+  D
Sbjct: 364 GMSEFYGILDSDWVRVRSQSFLQRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILD 423

Query: 446 NTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKANGEGIGVSLSSDCLGQQCV--WRAI 505
            T + L  C+    WS+    T + +    LCL++ G    V LS        +  W  I
Sbjct: 424 PTKVTLGLCNESQPWSYTPQNT-LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETI 483

Query: 506 SNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNGEPNCLQDTQSQWFELV 520
           S SN+ LA  + +  SLCL+V+   ++ ++ ++C C  GE +   D  SQWF++V
Sbjct: 484 SASNMLLAAKSTN-NSLCLDVDE--TNNLMASNCKCVKGEDSSC-DPISQWFKIV 504

[Close]

BLAST of Lsi09G015380 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.6e-89
Identity = 190/558 (34.05%), Postives = 295/558 (52.87%), Query Frame = 0


Query: 6   QAIVCLTFVFMLSSPLSYSL----PLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEG 65
           +AI    F+F+  S +S +L    PL T+ RWIV++K G RVKL C NWPSH + ++AEG
Sbjct: 3   KAIALTVFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEG 62

Query: 66  LNHRPLKELADEAIKLKFNCVCLTY----------ATHMTIEENFE-------------- 125
           L+ +P+  ++ +   + FNCV LT+          A ++T++++FE              
Sbjct: 63  LSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTH 122

Query: 126 --------------VVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWL 185
                          VV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  W+
Sbjct: 123 NPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWM 182

Query: 186 QGLSLVARRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNK 245
            GL  +A  F+N                       ++ V+ MSLRNE+RG    + DW K
Sbjct: 183 LGLKKMATIFMN-----------------------VKNVVGMSLRNELRGYNHTSKDWYK 242

Query: 246 YVTQGVTTIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDES 305
           Y+ +G   +H  NPNVLVI+SGLN+D DL  LK++P+ + +   KLV E+H YSF+    
Sbjct: 243 YMQKGAEAVHTSNPNVLVILSGLNFDADLSFLKDRPVNL-SFKKKLVLELHWYSFTDGTG 302

Query: 306 RYVQQPLNNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAH 365
           ++    +N+ C+ + +      GF+++    FPLF+SE+G DQRG +   NRYM+C  A 
Sbjct: 303 QWKSHNVNDFCSQMFSKERRTGGFVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAW 362

Query: 366 LALKDMDWALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPS 425
            A KD+DWA+W   G YY+REG+    E +G+L++NW  + N  + ++  ++Q     P 
Sbjct: 363 AAEKDLDWAVWAVTGVYYFREGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPG 422

Query: 426 SNASSSYVMYHPQSGQC--AQASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKA 485
              +    ++HP +G C   ++    + + L  C+    WS+  GG       +  CL+ 
Sbjct: 423 VKHNHHKKIFHPLTGLCLVRKSHCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEG 482

Query: 486 NGEGIGVSLSSDCLGQQCV-WRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCIC 519
               +G S+    LG+ C     IS + +HL+  T DG  +CL+V+S   + +V NSC C
Sbjct: 483 E-TAVGKSVK---LGRICTKIEQISATKMHLSFNTSDGSLVCLDVDS--DNNVVANSCNC 525

[Close]

BLAST of Lsi09G015380 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 7.2e-74
Identity = 182/556 (32.73%), Postives = 275/556 (49.46%), Query Frame = 0


Query: 10  CLTFVFMLS-SPLSYSLPLSTQGRWIVDSKTGRRVKLVCVNWPSHTQSMLAEGLNHRPLK 69
           CL F+F+ S S L+ S PLST+ RWIVD K G+RVKL CVNWP+H Q  +AEGL+ +PL 
Sbjct: 8   CLFFLFISSTSKLTTSYPLSTKSRWIVDEK-GQRVKLACVNWPAHLQPTVAEGLSKQPLD 67

Query: 70  ELADEAIKLKFNCVCLTY----------ATHMTIEENFE--------------------- 129
            ++ + + + FNCV LT+          A  +T++++FE                     
Sbjct: 68  SISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHL 127

Query: 130 -------VVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLSLVA 189
                   VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GL  +A
Sbjct: 128 PLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMA 187

Query: 190 RRFINKSTICNFKRFFIHFFKLTPTIRGLQTVIAMSLRNEIRGTMENANDWNKYVTQGVT 249
             F N          F H             VI MSLRNE RG  +  + W +++ QG  
Sbjct: 188 TLFRN----------FTH-------------VIGMSLRNEPRGARDYPDLWFRHMPQGAE 247

Query: 250 TIHNINPNVLVIVSGLNYDNDLRCLKEKPLTVNALDNKLVFEVHLYSFSGDESRYVQQPL 309
            +H  NP +LVI+SG+++D +L  L+++ + V+  D KLVFE+H YSFS     + +   
Sbjct: 248 AVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTD-KLVFELHWYSFSDGRDSWRKHNS 307

Query: 310 NNICANVINGFVDHAGFIMEGPNPFPLFVSEYGYDQRGTNDAENRYMSCFTAHLALKDMD 369
           N+ C  +I     + GF++     FPL +SE+G DQRG + + NRYM+C  A  A  D+D
Sbjct: 308 NDFCVKIIEKVTHNGGFLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLD 367

Query: 370 WALWTWQGSYYYREGEAEPTEVFGVLNSNWTQIQNPNFTKKFQLLQTMLQDPSSNASSSY 429
           WA+W   G YY R G                                    P+ N     
Sbjct: 368 WAVWALTGDYYLRTGPG--------------------------------LRPNKN----- 427

Query: 430 VMYHPQSGQCA--QASNDNTAIFLSNCSTPSQWSHGDGGTPIKMTTNGLCLKAN---GEG 489
           +++HP +G C     S++   + L  C     W+       + +  N +C++A    G+ 
Sbjct: 428 LLFHPSTGLCVTNNPSDNIPTLRLGPCPKSDPWTFNPSEGILWI--NKMCVEAPNVVGQK 487

Query: 490 IGVSLSSDC--LGQQCVWRAISNSNLHLATVTQDGKSLCLEVESSTSSKIVTNSCICTNG 520
           + + + + C  LGQ      IS + +HL+  T +G  LCL+V+   +S +V N C     
Sbjct: 488 VKLGVGTKCSKLGQ------ISATKMHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTM 488

The following BLAST results are available for this feature:

BLAST of Lsi09G015380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	9.1e-98	37.13	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of Lsi09G015380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6A6	1.4e-245	75.63	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459997 P...	[more]
A0A5A7TMC9	1.2e-233	71.51	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7THG1	1.0e-232	71.33	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7TMX0	1.7e-232	71.20	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1JZU8	6.5e-232	71.96	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=...	[more]

BLAST of Lsi09G015380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022958844.1	2.8e-245	75.63	uncharacterized protein LOC111459997 [Cucurbita moschata]	[more]
XP_038900453.1	2.5e-241	74.29	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
KAA0042711.1	2.5e-233	71.51	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
KAA0042713.1	2.1e-232	71.33	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
KAA0042709.1	3.6e-232	71.20	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]

BLAST of Lsi09G015380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13130.1	2.0e-103	36.66	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26130.1	1.9e-98	37.16	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	3.3e-95	35.70	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	1.6e-89	34.05	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	7.2e-74	32.73	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..381 e-value: 1.1E-57 score: 197.9
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 94..159 coord: 175..522 coord: 8..95
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 66..337 e-value: 3.4E-17 score: 62.8
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..367
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 389..499

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G015380.1	Lsi09G015380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds