Lsi09G015370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G015370
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr09: 23507004 .. 23509511 (-)
RNA-Seq ExpressionLsi09G015370
SyntenyLsi09G015370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGGACAACAATGGAAAGATATTGCTTTTGTTTGTTTTTTTGCATTGTTAACATTTAAGGCATATTCATTGCCTCTTTCAACCAGTGGAAGATGGATCATCGATGCTACAACTGGCCATCGCATGAAATTGATGTGCGTAAATTGGCCCGGACACATGCAAGGCATGTTGGCAGAGGGTCTTCATCTCAGGCCACTTGATGATATCGTTGCCTCAGTGGCAAAGTTTAGATTCAGTTGCGTGCGTTTGACATACTCAATACATATGTTCACACGCCATGCCAATTTGACTGTTCAACAATCTTTTGAGAACTTTGATTTGAAAGAAGCCATGACAGGCATAGCTCAAAACAATCCTTCTATAATGAACATGACACTCGTTGAGGCTTATGAAGCAGTAGTTGATTCACTTGTTGCACATGGAGTCATGGTAGTTTCTGACAATCATATAAGTCAACCAAGATGGTGTTGTAGTGATGATGATGGCAATGGCTTCTTCGGAGATCGCTATTTCGATCCTCAAGAATGGCTACAAGGAATCAGTTTGGCAGCTCAAAGACTAAAAAACAAACCCCAAGTATGTTTTATTTTTTAGAAAAATTTATTTATTTCATGATCATGATTGAGAACAATTATTATTTAAAATTTTTCATCTTAGACGGTTAAGTTATGTCCAAAAATGTTTTTTCTAAGACTTTTTTCAAACCCTAGCATTAATTCTATTCAACAAAAACCATTTATATATTTTGTCCACGAGGTTACAAACGACAACATGAATCCAACTGTGCAATTGTACAATCTTCTTCTCCTTTGATGATATTTAAGATGAGTTACTGTGTTATTACTTGACATTTTTTGAAGTCATAATCTCGTAGAGAACATATAAGACACAGTCAGAGTTCCTCAAGGTGCAACGTCAGAACTCATTCTATTTTCATTTTCATACTTTTAAACATAAAAAAATAGTCGTATTGCTAGTTTGAAGTTCATATTTATCAAACTTGGTAAAATAAAAATAGTTTCCATTTAAGAAAAGGCATCGTATATGTGATGTCTATGTGTTCTAAAAATTCATAGATGGAAGACTAATATAGACTATTTATTTTTAAAGAGTTAACCATAAACTAACGGTCGCAACTAATATTAATTAAGATTTGTTTAAATACACGAATTAAATTTGGGTAATAGTGACTGAAATGGAACACAATTCCCAAAATATAGGGACCAAATTAGTATTATCCAACATATAATTTATCATTGTTTTATATGATATATATTTTGACCATTTGCATGATAATATATATGCTAAAGGTGGTGGCAATGAGTTTGAGAAATGAACTGCGAGGACCAAACCAAAACGTGGAGATGTGGTTTCAATACATGAGCCAAGGAGCTAAGCTTATTCACCAAATTAACCCAAATGCTTTAGTCGTGGTTTCTGGGCTAAGCTACGACACCGATCTAAGCTTCCTGAAGAACAGGTCCATGGGCTTCAACTTAGACAACAAGCTTGTATTTGAGGCTCATTTGTACTCCTTTACAAACAACATGGGAGATTCTTGGACGTCGAAGCCACTAAACACATTTTGTTCTAATGTCAACCAAGGATTTGAAGACCGGGCTGGGTTTCTTGTAAGAGGACAAAACCCAATGCCTCTCTTTGTGAGTGAGTTTGGGATCGATCAAACAGGCGCAAATGAAGGCCAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACCGAGAATGATTTTGATTGGGGCCTATGGGCGTTGCAAGGGAGCTACTACTATAGGGAAGGTGTGAAAAATGCTGAAGAAACTTTTGGTGTCTTAGATTCCAATTTTGCACATGTCAAAAACCCTAAATTCCTCCAGAGGTTTCAACTGATGCAAACCAAACTTCAAGGTATAACTTTTAAAGGGATATGTTTCTTTTCTACCCTTACTTTTTTTTAATTATTTTTGTTTAATGCTTTAAAAAATTAAGAGGATTCGTTTCTACCCATTTTTTTAGATTCTATTTTTTGTCTCCTTACTAATTGAAGAATCAAAACGGCTGCTCTTTAACACAGATCCAAGCTCAAACCTCACAACATCATTCATAATGTACCATCCTCTTAGTGGCGAATGTGTGCGAATGAACAAAAAGTATCAATTAGGAGTCAGTAGCTGTAAAACCTCTAATCGTTGGAGCCATGAAGAAGATGGTGCTCCAATTAAGTTGGCAGGATCTATATTGTGCTTGAAAGCTGTTGGAGATGGGCTCCCTCCAATTCTCTCTAAAGAATGCTCGAGCCAACAAAGTGCTTGGAAATATGCTTCAAATGCCAAGCTTCAACTAGCCACTATTGATGAACAAGGACAAGTATTGTGCTTGCAAAGGACTTCTCATTCCCATCAAATTTTGACTAACAAGTGCATATGTCCGAATGATTCTCAATGCCTAGAAGATCCACAAAGCCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAATTAA

mRNA sequence

ATGACAGGACAACAATGGAAAGATATTGCTTTTGTTTGTTTTTTTGCATTGTTAACATTTAAGGCATATTCATTGCCTCTTTCAACCAGTGGAAGATGGATCATCGATGCTACAACTGGCCATCGCATGAAATTGATGTGCGTAAATTGGCCCGGACACATGCAAGGCATGTTGGCAGAGGGTCTTCATCTCAGGCCACTTGATGATATCGTTGCCTCAGTGGCAAAGTTTAGATTCAGTTGCGTGCGTTTGACATACTCAATACATATGTTCACACGCCATGCCAATTTGACTGTTCAACAATCTTTTGAGAACTTTGATTTGAAAGAAGCCATGACAGGCATAGCTCAAAACAATCCTTCTATAATGAACATGACACTCGTTGAGGCTTATGAAGCAGTAGTTGATTCACTTGTTGCACATGGAGTCATGGTAGTTTCTGACAATCATATAAGTCAACCAAGATGGTGTTGTAGTGATGATGATGGCAATGGCTTCTTCGGAGATCGCTATTTCGATCCTCAAGAATGGCTACAAGGAATCAGTTTGGCAGCTCAAAGACTAAAAAACAAACCCCAAGTGGTGGCAATGAGTTTGAGAAATGAACTGCGAGGACCAAACCAAAACGTGGAGATGTGGTTTCAATACATGAGCCAAGGAGCTAAGCTTATTCACCAAATTAACCCAAATGCTTTAGTCGTGGTTTCTGGGCTAAGCTACGACACCGATCTAAGCTTCCTGAAGAACAGGTCCATGGGCTTCAACTTAGACAACAAGCTTGTATTTGAGGCTCATTTGTACTCCTTTACAAACAACATGGGAGATTCTTGGACGTCGAAGCCACTAAACACATTTTGTTCTAATGTCAACCAAGGATTTGAAGACCGGGCTGGGTTTCTTGTAAGAGGACAAAACCCAATGCCTCTCTTTGTGAGTGAGTTTGGGATCGATCAAACAGGCGCAAATGAAGGCCAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACCGAGAATGATTTTGATTGGGGCCTATGGGCGTTGCAAGGGAGCTACTACTATAGGGAAGGTGTGAAAAATGCTGAAGAAACTTTTGGTGTCTTAGATTCCAATTTTGCACATGTCAAAAACCCTAAATTCCTCCAGAGGTTTCAACTGATGCAAACCAAACTTCAAGATCCAAGCTCAAACCTCACAACATCATTCATAATGTACCATCCTCTTAGTGGCGAATGTGTGCGAATGAACAAAAAGTATCAATTAGGAGTCAGTAGCTGTAAAACCTCTAATCGTTGGAGCCATGAAGAAGATGGTGCTCCAATTAAGTTGGCAGGATCTATATTGTGCTTGAAAGCTGTTGGAGATGGGCTCCCTCCAATTCTCTCTAAAGAATGCTCGAGCCAACAAAGTGCTTGGAAATATGCTTCAAATGCCAAGCTTCAACTAGCCACTATTGATGAACAAGGACAAGTATTGTGCTTGCAAAGGACTTCTCATTCCCATCAAATTTTGACTAACAAGTGCATATGTCCGAATGATTCTCAATGCCTAGAAGATCCACAAAGCCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAATTAA

Coding sequence (CDS)

ATGACAGGACAACAATGGAAAGATATTGCTTTTGTTTGTTTTTTTGCATTGTTAACATTTAAGGCATATTCATTGCCTCTTTCAACCAGTGGAAGATGGATCATCGATGCTACAACTGGCCATCGCATGAAATTGATGTGCGTAAATTGGCCCGGACACATGCAAGGCATGTTGGCAGAGGGTCTTCATCTCAGGCCACTTGATGATATCGTTGCCTCAGTGGCAAAGTTTAGATTCAGTTGCGTGCGTTTGACATACTCAATACATATGTTCACACGCCATGCCAATTTGACTGTTCAACAATCTTTTGAGAACTTTGATTTGAAAGAAGCCATGACAGGCATAGCTCAAAACAATCCTTCTATAATGAACATGACACTCGTTGAGGCTTATGAAGCAGTAGTTGATTCACTTGTTGCACATGGAGTCATGGTAGTTTCTGACAATCATATAAGTCAACCAAGATGGTGTTGTAGTGATGATGATGGCAATGGCTTCTTCGGAGATCGCTATTTCGATCCTCAAGAATGGCTACAAGGAATCAGTTTGGCAGCTCAAAGACTAAAAAACAAACCCCAAGTGGTGGCAATGAGTTTGAGAAATGAACTGCGAGGACCAAACCAAAACGTGGAGATGTGGTTTCAATACATGAGCCAAGGAGCTAAGCTTATTCACCAAATTAACCCAAATGCTTTAGTCGTGGTTTCTGGGCTAAGCTACGACACCGATCTAAGCTTCCTGAAGAACAGGTCCATGGGCTTCAACTTAGACAACAAGCTTGTATTTGAGGCTCATTTGTACTCCTTTACAAACAACATGGGAGATTCTTGGACGTCGAAGCCACTAAACACATTTTGTTCTAATGTCAACCAAGGATTTGAAGACCGGGCTGGGTTTCTTGTAAGAGGACAAAACCCAATGCCTCTCTTTGTGAGTGAGTTTGGGATCGATCAAACAGGCGCAAATGAAGGCCAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACCGAGAATGATTTTGATTGGGGCCTATGGGCGTTGCAAGGGAGCTACTACTATAGGGAAGGTGTGAAAAATGCTGAAGAAACTTTTGGTGTCTTAGATTCCAATTTTGCACATGTCAAAAACCCTAAATTCCTCCAGAGGTTTCAACTGATGCAAACCAAACTTCAAGATCCAAGCTCAAACCTCACAACATCATTCATAATGTACCATCCTCTTAGTGGCGAATGTGTGCGAATGAACAAAAAGTATCAATTAGGAGTCAGTAGCTGTAAAACCTCTAATCGTTGGAGCCATGAAGAAGATGGTGCTCCAATTAAGTTGGCAGGATCTATATTGTGCTTGAAAGCTGTTGGAGATGGGCTCCCTCCAATTCTCTCTAAAGAATGCTCGAGCCAACAAAGTGCTTGGAAATATGCTTCAAATGCCAAGCTTCAACTAGCCACTATTGATGAACAAGGACAAGTATTGTGCTTGCAAAGGACTTCTCATTCCCATCAAATTTTGACTAACAAGTGCATATGTCCGAATGATTCTCAATGCCTAGAAGATCCACAAAGCCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAATTAA

Protein sequence

MTGQQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNPSIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKLQLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHLN
Homology
BLAST of Lsi09G015370 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 3.4e-124
Identity = 232/535 (43.36%), Postives = 323/535 (60.37%), Query Frame = 0

Query: 17  LLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLHLRPLDDIVASVAK 76
           L+   ++SLPL T GRWI+D  TG R+KL CVNW GH++  L EGL+  P+  +  +++ 
Sbjct: 21  LVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISS 80

Query: 77  FRFSCVRLTYSIHMFTR--HANLTVQQSFENFDLKEAMTGIAQNNPSIMNMTLVEAYEAV 136
             F+CVRLTYSIHM TR  + N TV Q+F   +L EA +GI  NNP ++++  V AY  V
Sbjct: 81  LGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHV 140

Query: 137 VDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQRLKNKPQV 196
           V +L   GVMV+ DNH+S+P+WCC+ DDGNGFFGDRYF+P  W++G+ L A    N P V
Sbjct: 141 VAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNV 200

Query: 197 VAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGF 256
           VAMSLRNELRG       W ++M  GA  +H+ NP  LV++SGL +DTDLSFL    +  
Sbjct: 201 VAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL 260

Query: 257 NLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRGQN--PMPLFVS 316
               K+V+E H YSF    G  W +   N  C N    F+   GF+    N    PLF+S
Sbjct: 261 PFKEKIVYEGHWYSF----GVPWRTGLPNDVCKNETGRFKSNVGFVTSSANATAAPLFMS 320

Query: 317 EFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYR---EGVKNAEETFGVLD 376
           EFGIDQ   N+  NR+L+C   YL E D DW LW + GSYYYR   + VK+ EET+G  +
Sbjct: 321 EFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFN 380

Query: 377 SNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSF-IMYHPLSGECVRMNKKYQLGVSSCKT 436
            +++ ++NP F+ R + +Q  +QDP       + I+YHP SG CV       + + SC++
Sbjct: 381 HDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVESGIGNTVHLGSCQS 440

Query: 437 -SNRWSHEED-GAPIKLAGSILCLKAVGDGLPPILSKECSS-QQSAWKYASNAKLQLAT- 496
             +RW+++     PI L GS  C+   G+GLP I+++ CS+   + W   S+A+LQL T 
Sbjct: 441 VRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTR 500

Query: 497 ---IDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLE--DPQSQWFTLVPSNVHL 535
               D + + +CL   S S  I TN+CIC  DS C    +P+ QWF ++ +N  L
Sbjct: 501 VLGKDGKEKWMCLD-GSKSPLISTNECICITDSHCYPKLNPEKQWFKVITTNKQL 550

BLAST of Lsi09G015370 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-14
Identity = 78/373 (20.91%), Postives = 151/373 (40.48%), Query Frame = 0

Query: 29  TSGRWIIDATTGHRMKLMCVNWPG-HMQGMLAEGLHLRPLDDIVASVAKFRFSCVRLTYS 88
           T G  I+D  +G       +NW G         GL  R +DD++  V K  ++ +RL YS
Sbjct: 41  TQGNKIVD-ESGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 89  IHMFTRHANLTVQQSFENFDLKEAMTGIAQNNPSIMNMTLVEAYEAVVDSLVAHGVMVVS 148
                        Q F++    +++      NP ++ +  ++  + +++     G+ ++ 
Sbjct: 101 ------------NQLFDSSSRPDSID--YHKNPDLVGLNPIQIMDKLIEKAGQRGIQIIL 160

Query: 149 DNHISQPRWCCSDDDGNGFFGDRYFDPQ----EWLQGISLAAQRLKNKPQVVAMSLRNEL 208
           D H            G+G   + ++  Q     W+    + A R KN P V+   L NE 
Sbjct: 161 DRH----------RPGSGGQSELWYTSQYPESRWISDWKMLADRYKNNPTVIGADLHNEP 220

Query: 209 RGP------NQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDT-----------DLSF 268
            G       N + + W     +    I  +NPN L++V G+ ++            +L+ 
Sbjct: 221 HGQASWGTGNASTD-WRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTG 280

Query: 269 LKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRGQNP 328
           + N  +  ++ N++V+  H Y         W + P   F SN+   ++   G++ + QN 
Sbjct: 281 VANYPVVLDVPNRVVYSPHDYG-PGVSSQPWFNDP--AFPSNLPAIWDQTWGYISK-QNI 340

Query: 329 MPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFG 380
            P+ V EFG      +  + ++ +    Y+  N+  +  W+L           N+ +T G
Sbjct: 341 APVLVGEFGGRNVDLSCPEGKWQNALVHYIGANNLYFTYWSLN---------PNSGDTGG 374

BLAST of Lsi09G015370 vs. ExPASy TrEMBL
Match: A0A0A0KL32 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171770 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.7e-281
Identity = 462/533 (86.68%), Postives = 495/533 (92.87%), Query Frame = 0

Query: 4   QQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLH 63
           QQWK+IA VC F LLTFKAYSLPLST+GRWI+DATTG R+KLMCVNWPGHMQGMLAEGLH
Sbjct: 5   QQWKNIALVCVFVLLTFKAYSLPLSTNGRWIVDATTGQRVKLMCVNWPGHMQGMLAEGLH 64

Query: 64  LRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNPSIM 123
            RPLDDI++ VAK RF+CVRLTYSIHMFTRHANLTVQQSFENFD+K+AM GIAQNNPS++
Sbjct: 65  RRPLDDIISLVAKLRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDAMAGIAQNNPSLV 124

Query: 124 NMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISL 183
           N+TLVEAY AVVDSL AHGVMVVSDNHISQPRWCC++DDGNGFFGDRYFDP+EWLQGISL
Sbjct: 125 NLTLVEAYGAVVDSLAAHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPEEWLQGISL 184

Query: 184 AAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 243
           AAQ LK+K +VVAMS+RNE RGPNQNVE WFQYMSQGAKLIHQINPNALVVVSGLSYDTD
Sbjct: 185 AAQSLKSKAEVVAMSMRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 244

Query: 244 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRG 303
           LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGD W SKPLNTFC++VNQGFEDRAGFLVRG
Sbjct: 245 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRG 304

Query: 304 QNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEE 363
           QNPMPLFVSEFGIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE
Sbjct: 305 QNPMPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEE 364

Query: 364 TFGVLDSNFAHVKNPK-FLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQLG 423
            FGVLDS FA  KN K FLQRFQLMQTKLQDPSSN TTS IMYHPLSG CVRMNKKYQLG
Sbjct: 365 NFGVLDSTFAKAKNSKLFLQRFQLMQTKLQDPSSNFTTSLIMYHPLSGGCVRMNKKYQLG 424

Query: 424 VSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKLQL 483
           +SSCKTSNRW HE+D +PIKLAGS+LCLKA+G GLPPILS++CSSQQS WKY S+AKLQL
Sbjct: 425 ISSCKTSNRWIHEQDSSPIKLAGSVLCLKAIGVGLPPILSQDCSSQQSIWKYGSSAKLQL 484

Query: 484 ATIDEQGQVLCLQR-TSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHL 535
           AT+DEQGQ LCLQR  SHSHQI+TNKC+C NDSQC EDPQSQWFTLVPSN+ L
Sbjct: 485 ATVDEQGQALCLQRAASHSHQIVTNKCLCSNDSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G015370 vs. ExPASy TrEMBL
Match: A0A5A7TI19 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00580 PE=3 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 3.9e-280
Identity = 461/533 (86.49%), Postives = 492/533 (92.31%), Query Frame = 0

Query: 4   QQWKDIAFVCFFA-LLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGL 63
           QQWK+IA VC F  LLTFKAYSLPLST+GRWIIDATTG R+KLMCVNW GHMQGML EGL
Sbjct: 5   QQWKNIALVCVFVLLLTFKAYSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGMLVEGL 64

Query: 64  HLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNPSI 123
           H RPLDDI A VAK RF+CVRLTYSIHMFTRHAN+TV+QSFENFD+K+A+ GIAQNNPSI
Sbjct: 65  HRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANVTVKQSFENFDMKDALVGIAQNNPSI 124

Query: 124 MNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGIS 183
           +N+TLVEAY AVVDSL AHG+MVVSDNHISQPRWCC +DDGNGFFGDRYFDPQEW QGIS
Sbjct: 125 LNLTLVEAYGAVVDSLAAHGIMVVSDNHISQPRWCCDNDDGNGFFGDRYFDPQEWFQGIS 184

Query: 184 LAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDT 243
           LAAQ LK+K QVVAMSLRNE RGPNQNVE WFQYMSQGAKLIHQINPNALVVVSGLSYDT
Sbjct: 185 LAAQSLKSKAQVVAMSLRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDT 244

Query: 244 DLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVR 303
           DLSFL+NRSMGFNLDNKLVFEAHLYSFTNNM D W SKPLNTFC+++NQGFEDRAGFLVR
Sbjct: 245 DLSFLRNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVR 304

Query: 304 GQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAE 363
           GQNP+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAE
Sbjct: 305 GQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAE 364

Query: 364 ETFGVLDSNFAHVKNPK-FLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQL 423
           E FGVLDSNF   KN K FLQRFQLMQTKLQDPSSN TTSFIMYHPLSG C+RMNKKYQL
Sbjct: 365 ENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPSSNFTTSFIMYHPLSGGCMRMNKKYQL 424

Query: 424 GVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKLQ 483
           G+SSCKTSNRWSHE+DGAPIKLAGSILCLKA+G GLPPILS++CSSQQS WKY SNAKLQ
Sbjct: 425 GISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQDCSSQQSIWKYGSNAKLQ 484

Query: 484 LATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHL 535
           LAT+DEQGQ LCLQR SHSHQI+TNKC+C NDSQC EDPQSQWFTLVPSN+ L
Sbjct: 485 LATVDEQGQALCLQRASHSHQIVTNKCLCSNDSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G015370 vs. ExPASy TrEMBL
Match: A0A1S3CT43 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504654 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 9.5e-279
Identity = 464/535 (86.73%), Postives = 495/535 (92.52%), Query Frame = 0

Query: 1   MTGQ-QWKDIAFVCFFA-LLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGML 60
           M GQ Q K+IA VC F  LLTFKA+SLPLST+GRWIIDATTG R+KLMCVNW GHMQGML
Sbjct: 1   MKGQHQRKNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGML 60

Query: 61  AEGLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQN 120
            EGLH RPLDDI A VAK RF+CVRLTYSIHMFTRHANLTV+QSFENFD+K+AM GIAQN
Sbjct: 61  VEGLHRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQN 120

Query: 121 NPSIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWL 180
           NPSI+N+TLVEAY AVVDSLVAHG+MVVSDNHISQPRWCC ++DGNGFFGDRYFDPQEWL
Sbjct: 121 NPSILNLTLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWL 180

Query: 181 QGISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGL 240
           QGISLAAQ LK+K QVVAMSLRNELRGPNQNVE WFQYMSQGAKLIHQINPNALVVVSGL
Sbjct: 181 QGISLAAQSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGL 240

Query: 241 SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAG 300
           SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D W SKPLNTFC+++NQGFEDRAG
Sbjct: 241 SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAG 300

Query: 301 FLVRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGV 360
           FLVRGQNP+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GV
Sbjct: 301 FLVRGQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGV 360

Query: 361 KNAEETFGVLDSNFAHVKNPK-FLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNK 420
           KNAEE FGVLDSNF   KN K FLQRFQLMQTKLQDPSSN TT+FIMYHPLSG CVRMNK
Sbjct: 361 KNAEENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPSSNFTTTFIMYHPLSGGCVRMNK 420

Query: 421 KYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASN 480
           KYQLG+SSCKTSNRWSHE+DGAPIKLAGSILCLKA+G GLPPILS++CSSQQS W+YASN
Sbjct: 421 KYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKAIGVGLPPILSQDCSSQQSIWRYASN 480

Query: 481 AKLQLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNV 533
           AKLQLAT+DEQGQ LCLQR SHSHQI+TNKC+C  DSQC EDPQSQWFTLVPSN+
Sbjct: 481 AKLQLATVDEQGQALCLQRASHSHQIVTNKCLCTIDSQCQEDPQSQWFTLVPSNL 535

BLAST of Lsi09G015370 vs. ExPASy TrEMBL
Match: A0A5D3BM14 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00610 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 461/535 (86.17%), Postives = 493/535 (92.15%), Query Frame = 0

Query: 1   MTGQ-QWKDIAFVCFFA-LLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGML 60
           M GQ Q K+IA VC F  LLTFKA+SLPLST+GRWIIDATTG R+KLMCVNW GHMQGML
Sbjct: 1   MKGQHQRKNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGQRVKLMCVNWAGHMQGML 60

Query: 61  AEGLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQN 120
            EGLH RPLDDI A VAK RF+CVRLTYSIHMFTRHANLTV+QSFENFD+K+AM GIAQN
Sbjct: 61  VEGLHRRPLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQN 120

Query: 121 NPSIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWL 180
           NPSI+N+TLVEAY AVVDSLVAHG+MVVSDNHISQPRWCC ++DGNGFFGDRYFDPQEW 
Sbjct: 121 NPSILNLTLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWF 180

Query: 181 QGISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGL 240
           QGISLAAQ LK+K QVVAMSLRNELRGPNQNVE WFQYMSQGAKLIHQINPNALVVVSGL
Sbjct: 181 QGISLAAQSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGL 240

Query: 241 SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAG 300
           SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D W SKPLNTFC+++NQGFEDRAG
Sbjct: 241 SYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAG 300

Query: 301 FLVRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGV 360
           FLVRGQNP+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GV
Sbjct: 301 FLVRGQNPIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGV 360

Query: 361 KNAEETFGVLDSNFAHVKNPK-FLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNK 420
           KNAEE FGVLDSNF   KN K FLQRFQLMQTKLQDP+SN TT+FIMYHPLSG CVRMNK
Sbjct: 361 KNAEENFGVLDSNFTKAKNSKLFLQRFQLMQTKLQDPNSNFTTTFIMYHPLSGGCVRMNK 420

Query: 421 KYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASN 480
           KYQLG+SSCKTSNRWSHE+D APIKLAGSILCLKA+G GLPPILS++CSSQQS W+YASN
Sbjct: 421 KYQLGISSCKTSNRWSHEQDSAPIKLAGSILCLKAIGVGLPPILSQDCSSQQSIWRYASN 480

Query: 481 AKLQLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNV 533
           AKLQLAT+DEQGQ LCLQR SHSHQI+TNKC+C  DSQC EDPQSQWFTLVPSN+
Sbjct: 481 AKLQLATVDEQGQALCLQRASHSHQIVTNKCLCTIDSQCQEDPQSQWFTLVPSNL 535

BLAST of Lsi09G015370 vs. ExPASy TrEMBL
Match: A0A0A0KNB6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171760 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.9e-277
Identity = 457/533 (85.74%), Postives = 490/533 (91.93%), Query Frame = 0

Query: 4   QQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLH 63
           QQW++IA VC F  L  KA SLPLST+GRWI+DATTG+R+KLMCVNW GHMQGMLAEGLH
Sbjct: 5   QQWRNIALVCVFVFLISKACSLPLSTNGRWIVDATTGNRVKLMCVNWAGHMQGMLAEGLH 64

Query: 64  LRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNPSIM 123
           LRPLDDI A V K RF+CVRLTYSIHMFTRHANLTVQQSFENFD+K+A+ GIAQNNPSI+
Sbjct: 65  LRPLDDIAALVVKSRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDALAGIAQNNPSIL 124

Query: 124 NMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISL 183
           NMT+V+AY AV+DSL AH VMVVSDNHISQPRWCC++DDGNGFFGDRYFDPQEWLQGISL
Sbjct: 125 NMTVVQAYGAVIDSLAAHRVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPQEWLQGISL 184

Query: 184 AAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 243
           AAQ LK+K QVVAMSLRNE RGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTD
Sbjct: 185 AAQNLKSKSQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 244

Query: 244 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRG 303
           LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGD W SKPLNTFC++VNQGFEDRAGFLVRG
Sbjct: 245 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRG 304

Query: 304 QNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEE 363
           QNP+PLFVSEFGIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE
Sbjct: 305 QNPIPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEE 364

Query: 364 TFGVLDSNFAHVKNPK-FLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQLG 423
            FGVLDS FA  KN K FLQRFQLMQTKLQDPSSN TTS IMYHPLSG CVRMNKKYQLG
Sbjct: 365 NFGVLDSTFAKAKNSKLFLQRFQLMQTKLQDPSSNFTTSLIMYHPLSGGCVRMNKKYQLG 424

Query: 424 VSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKLQL 483
           +SSCKTSNRW HE+D +PIKLAGS+LCLKA+G GLPPILS++CSSQQS WKY SNAKLQL
Sbjct: 425 ISSCKTSNRWIHEQDSSPIKLAGSVLCLKAIGVGLPPILSQDCSSQQSIWKYGSNAKLQL 484

Query: 484 ATIDEQGQVLCLQR-TSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHL 535
           ATIDEQGQ LCLQR  SHSHQ++TNKC+C +DSQC EDPQSQWFTLVPSN+ L
Sbjct: 485 ATIDEQGQALCLQRAASHSHQLVTNKCLCSSDSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G015370 vs. NCBI nr
Match: KAE8648503.1 (hypothetical protein Csa_009407 [Cucumis sativus])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.1e-289
Identity = 470/535 (87.85%), Postives = 505/535 (94.39%), Query Frame = 0

Query: 1   MTGQQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAE 60
           MTGQ WK++A  C F LLTFKAYSLPLST+GRWI++ATTG R+KLMCVNWPGHMQ M+AE
Sbjct: 1   MTGQPWKNLALACVFVLLTFKAYSLPLSTNGRWIVEATTGQRVKLMCVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNP 120
           GLHLRPLDDI   VA  RF+CVRLTYSIHMFTR+ANLTV+QSFENFD+KEA+ GIAQNNP
Sbjct: 61  GLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNP 120

Query: 121 SIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQG 180
           +I+NM +VEAYEAVVDSL AHGVMVVSDNHISQPRWCCS+DDGNGFFGDRYF+ QEWL+G
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEG 180

Query: 181 ISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSY 240
           +SLAAQ LKNKPQVVAMSLRNE RGPNQNVEMWFQYMSQGAKL+HQINPNALVVVSGLSY
Sbjct: 181 LSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFL 300
           DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D WTSKPLNTFC+NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 VRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
           VRGQNP+PLFVSEFGI+Q GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420
            EETFGVLD+ F +VKNPKFLQ+FQLMQTKLQDPSSNLTTSFIMYHPLSGECV++NKKYQ
Sbjct: 361 DEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMYHPLSGECVQVNKKYQ 420

Query: 421 LGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKL 480
           LGVSSCKTSNRWSHE+DG PIKL GSILCL+AVGDGLPPILSK+CSSQQSAWKYASNAKL
Sbjct: 421 LGVSSCKTSNRWSHEQDGTPIKLVGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKL 480

Query: 481 QLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHLN 536
           QLAT+DE+GQ LCLQR SHSHQILTNKC+CPNDS+C  DPQSQWFTLVPSNVHLN
Sbjct: 481 QLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWFTLVPSNVHLN 535

BLAST of Lsi09G015370 vs. NCBI nr
Match: KAE8648505.1 (hypothetical protein Csa_007897 [Cucumis sativus])

HSP 1 Score: 1002.3 bits (2590), Expect = 1.6e-288
Identity = 469/532 (88.16%), Postives = 502/532 (94.36%), Query Frame = 0

Query: 1   MTGQQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAE 60
           MTGQ WK++A  C F LLTFKAYSLPLST+GRWI++ATTG R+KLMCVNWPGHMQ M+AE
Sbjct: 1   MTGQPWKNLALACVFVLLTFKAYSLPLSTNGRWIVEATTGQRVKLMCVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNP 120
           GLHLRPLDDI   VA  RF+CVRLTYSIHMFTR+ANLTV+QSFENFD+KEA+ GIAQNNP
Sbjct: 61  GLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNP 120

Query: 121 SIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQG 180
           +I+NM +VEAYEAVVDSL AHGVMVVSDNHISQPRWCCS+DDGNGFFGDRYF+ QEWL+G
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEG 180

Query: 181 ISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSY 240
           +SLAAQ LKNKPQVVAMSLRNE RGPNQNVEMWFQYMSQGAKL+HQINPNALVVVSGLSY
Sbjct: 181 LSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFL 300
           DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM D WTSKPLNTFC+NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 VRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
           VRGQNP+PLFVSEFGI+Q GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420
            EETFGVLDS F +VKNPKFLQ+FQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ
Sbjct: 361 DEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420

Query: 421 LGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKL 480
           LGVSSCKTSNRWSHE+DG PIKL GSILCL+AVGDGLPPILSK+CSSQQSAWKYASNAKL
Sbjct: 421 LGVSSCKTSNRWSHEQDGTPIKLVGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKL 480

Query: 481 QLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNV 533
           QLAT+DE+GQ LCLQR SHSHQILTNKC+CPNDS+C  DPQSQWFTLVPSN+
Sbjct: 481 QLATVDEEGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWFTLVPSNL 532

BLAST of Lsi09G015370 vs. NCBI nr
Match: XP_031739082.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical protein Csa_010408 [Cucumis sativus])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.1e-288
Identity = 468/535 (87.48%), Postives = 504/535 (94.21%), Query Frame = 0

Query: 1   MTGQQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAE 60
           M+GQQWK+++  C F LLTF+AYSLPLST+GRWI++ATTG R+KL+CVNWPGHMQ M+AE
Sbjct: 1   MSGQQWKNVSLACVFVLLTFEAYSLPLSTNGRWIVEATTGQRVKLICVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNP 120
           GLHL+PLDDI A V K RF+CVRLTYSIHMFTR+ANLTV+QSFENFDLKEA+ GIAQNNP
Sbjct: 61  GLHLKPLDDIAAMVVKLRFNCVRLTYSIHMFTRYANLTVKQSFENFDLKEAIVGIAQNNP 120

Query: 121 SIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQG 180
           +I+NM +VEAYEAVVDSL AHGVMVVSDNHISQPRWCCS+DDGNGFFGDRYF+ QEWLQG
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLQG 180

Query: 181 ISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSY 240
           +SLA Q LK KPQVVAMSLRNE RGPNQNVEMWFQYMSQG KL+HQINPNALVVVSGLSY
Sbjct: 181 LSLATQSLKTKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGTKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFL 300
           DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGD WTSKPLNTFC+NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 VRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
           VRGQNP+PLFVSEFGI+Q G NEGQNRFLSCFFTYLT+NDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGVNEGQNRFLSCFFTYLTKNDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420
            EETFGVLDS F +VKNPKFLQ+FQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ
Sbjct: 361 DEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420

Query: 421 LGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKL 480
           LGVSSCKTSNRWSHE+D  PIKLAGSILCL+AVGDGLPPILSK+CSSQQSAWKYASNAKL
Sbjct: 421 LGVSSCKTSNRWSHEQDDTPIKLAGSILCLQAVGDGLPPILSKDCSSQQSAWKYASNAKL 480

Query: 481 QLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHLN 536
           QLAT+DEQGQ LCLQR SHSHQILTNKC+CPNDS+C  DPQSQWFTLVPSNVHL+
Sbjct: 481 QLATVDEQGQALCLQRASHSHQILTNKCMCPNDSECQGDPQSQWFTLVPSNVHLS 535

BLAST of Lsi09G015370 vs. NCBI nr
Match: XP_038874277.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 978.0 bits (2527), Expect = 3.2e-281
Identity = 462/535 (86.36%), Postives = 495/535 (92.52%), Query Frame = 0

Query: 1   MTGQQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAE 60
           MT  QWK+IA VC F LLTFKAYSLPLST+GRWI+DA TG RMKLMCVNWPGHMQ MLAE
Sbjct: 1   MTKHQWKNIALVCIFVLLTFKAYSLPLSTNGRWIVDAATGQRMKLMCVNWPGHMQVMLAE 60

Query: 61  GLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNP 120
           GLHLRPLDDI   VAK RF+CVRLTYSIHMFTR+ANLTVQQSFENFDLKEAM GIA+NNP
Sbjct: 61  GLHLRPLDDIAVLVAKLRFNCVRLTYSIHMFTRYANLTVQQSFENFDLKEAMAGIARNNP 120

Query: 121 SIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQG 180
           SI+NMTL+EAY+AVVDSL  HGVMVVSDNHISQPRWCC++DDGNGFFGDRYFDPQEWLQG
Sbjct: 121 SILNMTLIEAYKAVVDSLAVHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPQEWLQG 180

Query: 181 ISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSY 240
           ISLAAQ LK+K QVVAMSLRNE RGPNQNVEMWFQYMSQGAKLIHQ NP+ALVV+SGLSY
Sbjct: 181 ISLAAQSLKSKFQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLIHQNNPDALVVISGLSY 240

Query: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFL 300
           DTDLSFLKNRS+GFNL+NKLVFEAHLYSFTNNM DSWTSKPLNTFC+NVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSLGFNLNNKLVFEAHLYSFTNNMSDSWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 VRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
           VRGQNP PLFVSEFGIDQTG N+GQNR+LSCFFTYL ENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPTPLFVSEFGIDQTGKNDGQNRYLSCFFTYLVENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ 420
           AEE+FGV D NF H KNPKFLQRFQLMQTKLQDPSSNLT SFIMYH LSGECVRM+KKYQ
Sbjct: 361 AEESFGVSDFNFRHAKNPKFLQRFQLMQTKLQDPSSNLTASFIMYHSLSGECVRMDKKYQ 420

Query: 421 LGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKL 480
           L VSSCKTSNRW+H+EDGAPIKL  S+LCLKA+G+GLPPILSK+CSSQQS WKYASNAKL
Sbjct: 421 LRVSSCKTSNRWNHKEDGAPIKLVDSLLCLKALGEGLPPILSKDCSSQQSVWKYASNAKL 480

Query: 481 QLATIDEQGQVLCLQRTSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHLN 536
           QLAT+D+QGQ LCLQ  SHSHQI+TNKC+C +DSQC  DPQSQWFTLV SNV+LN
Sbjct: 481 QLATVDKQGQALCLQSVSHSHQIVTNKCMCLDDSQCQGDPQSQWFTLVFSNVNLN 535

BLAST of Lsi09G015370 vs. NCBI nr
Match: KGN50395.1 (hypothetical protein Csa_000438 [Cucumis sativus])

HSP 1 Score: 977.2 bits (2525), Expect = 5.5e-281
Identity = 462/533 (86.68%), Postives = 495/533 (92.87%), Query Frame = 0

Query: 4   QQWKDIAFVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLH 63
           QQWK+IA VC F LLTFKAYSLPLST+GRWI+DATTG R+KLMCVNWPGHMQGMLAEGLH
Sbjct: 5   QQWKNIALVCVFVLLTFKAYSLPLSTNGRWIVDATTGQRVKLMCVNWPGHMQGMLAEGLH 64

Query: 64  LRPLDDIVASVAKFRFSCVRLTYSIHMFTRHANLTVQQSFENFDLKEAMTGIAQNNPSIM 123
            RPLDDI++ VAK RF+CVRLTYSIHMFTRHANLTVQQSFENFD+K+AM GIAQNNPS++
Sbjct: 65  RRPLDDIISLVAKLRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDAMAGIAQNNPSLV 124

Query: 124 NMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISL 183
           N+TLVEAY AVVDSL AHGVMVVSDNHISQPRWCC++DDGNGFFGDRYFDP+EWLQGISL
Sbjct: 125 NLTLVEAYGAVVDSLAAHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPEEWLQGISL 184

Query: 184 AAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 243
           AAQ LK+K +VVAMS+RNE RGPNQNVE WFQYMSQGAKLIHQINPNALVVVSGLSYDTD
Sbjct: 185 AAQSLKSKAEVVAMSMRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTD 244

Query: 244 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRG 303
           LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGD W SKPLNTFC++VNQGFEDRAGFLVRG
Sbjct: 245 LSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRG 304

Query: 304 QNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEE 363
           QNPMPLFVSEFGIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE
Sbjct: 305 QNPMPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEE 364

Query: 364 TFGVLDSNFAHVKNPK-FLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQLG 423
            FGVLDS FA  KN K FLQRFQLMQTKLQDPSSN TTS IMYHPLSG CVRMNKKYQLG
Sbjct: 365 NFGVLDSTFAKAKNSKLFLQRFQLMQTKLQDPSSNFTTSLIMYHPLSGGCVRMNKKYQLG 424

Query: 424 VSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECSSQQSAWKYASNAKLQL 483
           +SSCKTSNRW HE+D +PIKLAGS+LCLKA+G GLPPILS++CSSQQS WKY S+AKLQL
Sbjct: 425 ISSCKTSNRWIHEQDSSPIKLAGSVLCLKAIGVGLPPILSQDCSSQQSIWKYGSSAKLQL 484

Query: 484 ATIDEQGQVLCLQR-TSHSHQILTNKCICPNDSQCLEDPQSQWFTLVPSNVHL 535
           AT+DEQGQ LCLQR  SHSHQI+TNKC+C NDSQC EDPQSQWFTLVPSN+ L
Sbjct: 485 ATVDEQGQALCLQRAASHSHQIVTNKCLCSNDSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G015370 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 433.7 bits (1114), Expect = 2.1e-121
Identity = 223/513 (43.47%), Postives = 312/513 (60.82%), Query Frame = 0

Query: 26  PLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLHLRPLDDIVASVAKFRFSCVRLT 85
           PLST+ RWIID   G R+KL CVNWP H+Q ++AEGL  + +DD+   +    F+CVR T
Sbjct: 4   PLSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YSIHMFTRHA---NLTVQQSFENFDLKEAMTGIAQNNPSIMNMTLVEAYEAVVDSLVAHG 145
           + + + T      N+TV+QSF++  L + ++G    NPS++++ L+EAY+ VV  L  + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 VMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQRLKNKPQVVAMSLRNE 205
           VMV+ DNH+++P WCC  +DGNGFFGD +FDP  W+ G++  A   K    VV MSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 LRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVF 265
           LRGP QNV+ WF+YM QGA+ +H+ NPN LV++SGLSYDTDLSF+++R +      KLVF
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVF 243

Query: 266 EAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRGQNPMPLFVSEFGIDQTGAN 325
           E H YSFTN   ++W+SK  N  C  + +  E+  GF +R     P+F+SEFGID  G N
Sbjct: 244 ELHRYSFTNT--NTWSSKNPNEACGEILKSIENGGGFNLR---DFPVFLSEFGIDLRGKN 303

Query: 326 EGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSNFAHVKNPKFLQ 385
              NR++ C   +  END DW +W LQGSYY REGV    E +G+LDS++  V++  FLQ
Sbjct: 304 VNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQ 363

Query: 386 RFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMN----KKYQLGVSSCKTSNRWSHEEDG 445
           R  L+ + LQ P S      +++HPL+G C+  +     K  LG+  C  S  WS+    
Sbjct: 364 RLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGL--CNESQPWSYTPQN 423

Query: 446 APIKLAGSILCLKAVGDGLPPILSK-ECSSQQ-SAWKYASNAKLQLATIDEQGQVLCLQR 505
             + L    LCL++ G   P  LS+  CSS   S W+  S + + LA        LCL  
Sbjct: 424 T-LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAA-KSTNNSLCLDV 483

Query: 506 TSHSHQILTN-KCICPNDSQCLEDPQSQWFTLV 529
              ++ + +N KC+   DS C  DP SQWF +V
Sbjct: 484 DETNNLMASNCKCVKGEDSSC--DPISQWFKIV 504

BLAST of Lsi09G015370 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 430.3 bits (1105), Expect = 2.3e-120
Identity = 220/543 (40.52%), Postives = 321/543 (59.12%), Query Frame = 0

Query: 1   MTGQQWKDIAFV----CFF----ALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPG 60
           MT ++ K + F+    CFF    A  T    S PLSTS RWI+D   G R+KL+C NWP 
Sbjct: 1   MTSKEPKHMFFIFPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPS 60

Query: 61  HMQGMLAEGLHLRPLDDIVASVAKFRFSCVRLTYSIHMFTRHA---NLTVQQSFENFDLK 120
           H+Q ++AEGL  +P+D +   + +  F+CVRLT+ + + T      N+TV+QSF++  L 
Sbjct: 61  HLQPVVAEGLSKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLN 120

Query: 121 EAMTGIAQNNPSIMNMTLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGD 180
           + + G   NNPSI+++ L+EAY+ VV +L  + VMV+ DNH+++P WCC++DDGNGFFGD
Sbjct: 121 DDIVGFQTNNPSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGD 180

Query: 181 RYFDPQEWLQGISLAAQRLKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINP 240
           ++FDP  W+  +   A        VV MSLRNELRGP QNV  WF+YM QGA+ +H  N 
Sbjct: 181 QFFDPTVWVAALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANN 240

Query: 241 NALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNV 300
             LV++SGLS+D DLSF+++R +  +   KLVFE H YSF++  G+SW +   N  C  V
Sbjct: 241 KVLVILSGLSFDADLSFVRSRPVKLSFTGKLVFELHWYSFSD--GNSWAANNPNDICGRV 300

Query: 301 NQGFEDRAGFLVRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQ 360
                +  G+L+      PLF+SEFGID+ G N   NR+  C   +  END DW LWAL 
Sbjct: 301 LNRIGNGGGYLL--NQGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALT 360

Query: 361 GSYYYREGVKNAEETFGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLS 420
           GSYY R+G     E +GVLDS++  V+N  FLQ+   +Q+ LQ P        +++HPL+
Sbjct: 361 GSYYLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLT 420

Query: 421 GECV--RMNKKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSK-ECS 480
           G C+   ++    L +  C +S  WS+ +    IK     LCL++ G   P  +++  CS
Sbjct: 421 GLCIVRSLDDPKMLTLGPCNSSEPWSYTKKALRIK--DQQLCLQSNGPKNPVTMTRTSCS 480

Query: 481 SQQSAWKYASNAKLQLATIDEQGQVLCLQRTSHSHQILTNKCIC-PNDSQCLEDPQSQWF 529
           +  S W+  S +++ LA+       LCL     ++ ++ N C C   D  C  +P SQWF
Sbjct: 481 TSGSKWQTISASRMHLASTTSNKTSLCLD-VDTANNVVANACKCLSKDKSC--EPMSQWF 533

BLAST of Lsi09G015370 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 429.1 bits (1102), Expect = 5.2e-120
Identity = 226/528 (42.80%), Postives = 310/528 (58.71%), Query Frame = 0

Query: 11  FVCFFAL-LTFKAYSLPLSTSGRWII-DATTGHRMKLMCVNWPGHMQGMLAEGLHLRPLD 70
           F+  F L      ++ P ST  RWI+ D   G R+KL CVNWP H++  +AEGL  +PLD
Sbjct: 6   FISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLD 65

Query: 71  DIVASVAKFRFSCVRLTYSIHMFTRH---ANLTVQQSFENFDLKEAMTGIAQNNPSIMNM 130
            I   +    F+CVRLT+ +++ T     A +TV+QS   F L EA++G   +NP+I+++
Sbjct: 66  AIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDL 125

Query: 131 TLVEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAA 190
            L++A++ VV  L  H VMV+ DNHISQP WCCSD+DGNGFFGD++ +PQ W++G+   A
Sbjct: 126 PLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMA 185

Query: 191 QRLKN-KPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTDL 250
               N    VV MSLRNELRGP QN++ W++YM +GA+ +H +NPN LV+VSGL+Y TDL
Sbjct: 186 SMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDL 245

Query: 251 SFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRGQ 310
           SFL+ R    +   K+VFE H Y F N    +W    LN  C    +     +GFL+  +
Sbjct: 246 SFLRERPFEVSFRRKVVFEIHWYGFWN----TWEGDNLNKICGKETEKMMKMSGFLL--E 305

Query: 311 NPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEET 370
             +PLFVSEFGIDQ G N   N+FLSCF     + D DW LW L GSYY RE    ++E+
Sbjct: 306 KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDES 365

Query: 371 FGVLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQLGVS 430
           +GVLD N++ ++N   LQ    +QT             IM+HP +G C+     +QL + 
Sbjct: 366 YGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK-IMFHPSTGLCIVRKSLFQLKLG 425

Query: 431 SCKTSNRWSHEEDGAPIKLAGSILCLKAVGDGLPPILSKECS-SQQSAWKYASNAKLQLA 490
           SC  S  W              ILCLKA   G    L    S S  S WK  S++K+QL+
Sbjct: 426 SCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFSDSKMQLS 485

Query: 491 TIDEQGQVLCLQRTSHSHQILTNKCIC-PNDSQCLEDPQSQWFTLVPS 531
           +I + G  +CL   + ++ I+TN C C   +S C  DP+SQWF LV S
Sbjct: 486 SITKNGFSVCLDVDTENNNIVTNSCKCLRGNSSC--DPRSQWFKLVTS 524

BLAST of Lsi09G015370 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 369.0 bits (946), Expect = 6.4e-102
Identity = 202/525 (38.48%), Postives = 295/525 (56.19%), Query Frame = 0

Query: 14  FFALLTFK-AYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLHLRPLDDIVA 73
           F +L++   A   PL T  RWI++   GHR+KL C NWP H++ ++AEGL  +P+D I  
Sbjct: 14  FLSLISLTLATDYPLFTKSRWIVN-NKGHRVKLACANWPSHLKPVVAEGLSSQPMDSISK 73

Query: 74  SVAKFRFSCVRLTYSIHMF---TRHANLTVQQSFENFDLKEAMTGIAQNNPSIMNMTLVE 133
            +    F+CVRLT+ + +    T   N+TV+QSFE + L   + GI  +NP I+N  L+ 
Sbjct: 74  KIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNTPLIN 133

Query: 134 AYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQRLK 193
            ++AVV SL  H VMV+ DNH + P WCCS+DD + FFGD  F+P  W+ G+   A    
Sbjct: 134 VFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMATIFM 193

Query: 194 NKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKN 253
           N   VV MSLRNELRG N   + W++YM +GA+ +H  NPN LV++SGL++D DLSFLK+
Sbjct: 194 NVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLSFLKD 253

Query: 254 RSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLVRGQNPMPL 313
           R +  +   KLV E H YSFT+  G  W S  +N FCS +        GF++      PL
Sbjct: 254 RPVNLSFKKKLVLELHWYSFTDGTG-QWKSHNVNDFCSQMFSKERRTGGFVL--DQGFPL 313

Query: 314 FVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLD 373
           F+SEFG DQ G +   NR+++C   +  E D DW +WA+ G YY+REG +   E +G+LD
Sbjct: 314 FLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEAYGMLD 373

Query: 374 SNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGEC-VRMN--KKYQLGVSSC 433
           +N+ +V N  +L+R  ++Q     P         ++HPL+G C VR +   + +L +  C
Sbjct: 374 ANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESELTLGPC 433

Query: 434 KTSNRWSHEEDGAPIKLAGSILCLK---AVGDGLPPILSKECSSQQSAWKYASNAKLQLA 493
                WS+   G      G   CL+   AVG  +   L + C+  +      S  K+ L+
Sbjct: 434 TKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVK--LGRICTKIEQ----ISATKMHLS 493

Query: 494 TIDEQGQVLCLQRTSHSHQILTNKCIC-PNDSQCLEDPQSQWFTL 528
                G ++CL   S  + ++ N C C   D+ C  +P SQWF +
Sbjct: 494 FNTSDGSLVCLDVDS-DNNVVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of Lsi09G015370 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 329.3 bits (843), Expect = 5.6e-90
Identity = 188/529 (35.54%), Postives = 277/529 (52.36%), Query Frame = 0

Query: 11  FVCFFALLTFKAYSLPLSTSGRWIIDATTGHRMKLMCVNWPGHMQGMLAEGLHLRPLDDI 70
           F  F +  +    S PLST  RWI+D   G R+KL CVNWP H+Q  +AEGL  +PLD I
Sbjct: 10  FFLFISSTSKLTTSYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSI 69

Query: 71  VASVAKFRFSCVRLTYSIHMFTRHA---NLTVQQSFENFDLKEAMTGIAQNNPSIMNMTL 130
              +    F+CVRLT+ + + T       +TV+QSFE+  L E + GI  +NP ++++ L
Sbjct: 70  SKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPL 129

Query: 131 VEAYEAVVDSLVAHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQR 190
             A++ VV +L  +GVMV+ DNH++ P WCC D+D + FFG  +FDP  W +G+   A  
Sbjct: 130 FNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATL 189

Query: 191 LKNKPQVVAMSLRNELRGPNQNVEMWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFL 250
            +N   V+ MSLRNE RG     ++WF++M QGA+ +H  NP  LV++SG+ +DT+LSFL
Sbjct: 190 FRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFL 249

Query: 251 KNRSMGFNLDNKLVFEAHLYSFTNNMGDSWTSKPLNTFCSNVNQGFEDRAGFLV-RGQNP 310
           ++RS+  +  +KLVFE H YSF++   DSW     N FC  + +      GFL+ RG   
Sbjct: 250 RDRSVNVSFTDKLVFELHWYSFSDGR-DSWRKHNSNDFCVKIIEKVTHNGGFLLGRG--- 309

Query: 311 MPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFG 370
            PL +SEFG DQ G +   NR+++C   +  END DW +WAL G YY R G         
Sbjct: 310 FPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTGPG------- 369

Query: 371 VLDSNFAHVKNPKFLQRFQLMQTKLQDPSSNLTTSFIMYHPLSGECVRMNKKYQ---LGV 430
                                      P+ NL     ++HP +G CV  N       L +
Sbjct: 370 -------------------------LRPNKNL-----LFHPSTGLCVTNNPSDNIPTLRL 429

Query: 431 SSCKTSNRWSHEEDGAPIKLAGSILCLKA---VGDGLPPILSKECSSQQSAWKYASNAKL 490
             C  S+ W+       + +  + +C++A   VG  +   +  +CS         S  K+
Sbjct: 430 GPCPKSDPWTFNPSEGILWI--NKMCVEAPNVVGQKVKLGVGTKCSKLGQ----ISATKM 488

Query: 491 QLATIDEQGQVLCLQRTSHSHQILTNKC-ICPNDSQCLEDPQSQWFTLV 529
            L+     G +LCL      + ++ N+C     D+ C  DP SQWF ++
Sbjct: 490 HLSFKTSNGLLLCLDVDERDNSVVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA03.4e-12443.36Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P235481.7e-1420.91Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KL322.7e-28186.68Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171770 PE=3... [more]
A0A5A7TI193.9e-28086.49Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3CT439.5e-27986.73Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504654 PE=3 SV... [more]
A0A5D3BM143.1e-27786.17Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A0A0KNB68.9e-27785.74Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171760 PE=3... [more]
Match NameE-valueIdentityDescription
KAE8648503.11.1e-28987.85hypothetical protein Csa_009407 [Cucumis sativus][more]
KAE8648505.11.6e-28888.16hypothetical protein Csa_007897 [Cucumis sativus][more]
XP_031739082.12.1e-28887.48glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical... [more]
XP_038874277.13.2e-28186.36glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
KGN50395.15.5e-28186.68hypothetical protein Csa_000438 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G26140.12.1e-12143.47Cellulase (glycosyl hydrolase family 5) protein [more]
AT1G13130.12.3e-12040.52Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.15.2e-12042.80Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.16.4e-10238.48Glycosyl hydrolase superfamily protein [more]
AT5G16700.15.6e-9035.54Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 67..349
e-value: 9.1E-23
score: 81.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 25..392
e-value: 2.3E-69
score: 236.3
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 9..529
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 26..378
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 399..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G015370.1Lsi09G015370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds