Lsi09G015310 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi09G015310 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.2e-40
Identity = 89/238 (37.39%), Postives = 143/238 (60.08%), Query Frame = 0

Query: 58  QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVH 117
           Q +  +IQ  I+EAL     L+++ + LVG+ RTD GVHA GQVAHF    +Y  L  +H
Sbjct: 21  QPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQVAHFHFQ-DYIDLSRLH 80

Query: 118 AALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLN 177
            +LNGLLP DIR++ + P +P FH+++S + K YHY ++   ++DPFQR Y++H   K++
Sbjct: 81  VSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKVMDPFQRLYSWHFQRKID 140

Query: 178 YAAMREAANYFVGKHDFSAFTNAAHNDRV--GPL--------------VQLEVEGSGFLY 237
              +++AA  F G HDF++F N AH       P+              ++LE EG GFLY
Sbjct: 141 VNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIKPNEGGLRLEFEGDGFLY 200

Query: 238 RQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDH 280
           + VRN+VG L+ +       + + +I A+++R++ +  A   PP+GL L+ + Y+ ++
Sbjct: 201 KMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---PPEGLFLIQVFYENEN 252

BLAST of Lsi09G015310 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.5e-40
Identity = 95/227 (41.85%), Postives = 124/227 (54.63%), Query Frame = 0

Query: 62  PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVHAALN 121
           PTIQ  +E  L    +L    L + GASRTD GVHA GQV +F+T       E + AALN
Sbjct: 27  PTIQETLERCL---AELSGAALKVTGASRTDAGVHARGQVVNFVTGRWGIPTERIPAALN 86

Query: 122 GLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLNYAAM 181
           G+LP DI   +      DFHAR+S ++K Y Y +YN  +  PFQR Y+ H    L+  AM
Sbjct: 87  GVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHPVRSPFQRLYSLHVPRALDLRAM 146

Query: 182 REAANYFVGKHDFSAFTNA-------------AHNDRVGPLVQLEVEGSGFLYRQVRNMV 241
           R AA + +G+HDFS F  A             A  +   PLV+L     GFLY  VR M 
Sbjct: 147 RLAARHLMGRHDFSTFQAAGRPVQSAVRTLTRADIEAQAPLVRLVFRADGFLYNMVRIMA 206

Query: 242 GLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINY 276
           G L+++G E + P+ +P I+AS DR   A+    VPP GLCL  + Y
Sbjct: 207 GTLLEVGLERLDPEALPDIIASGDR---ARAGPTVPPHGLCLEEVEY 247

BLAST of Lsi09G015310 vs. ExPASy Swiss-Prot
Match: A3DJK8 (tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=truA PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 3.9e-37
Identity = 92/231 (39.83%), Postives = 129/231 (55.84%), Query Frame = 0

Query: 58  QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVH 117
           Q++  T+Q  IE A+ G T    + + L+GASRTD GVHA GQVA+FIT       +   
Sbjct: 21  QKNAKTVQDTIERAIKGLT---GEKVDLIGASRTDFGVHALGQVANFITNSGIPG-DRFS 80

Query: 118 AALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLN 177
            ALN +LP DI ++E      DFHAR+    K Y Y IYN        R  TYH  +KL+
Sbjct: 81  YALNRMLPDDIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFPSALLRHRTYHVSHKLD 140

Query: 178 YAAMREAANYFVGKHDFSAFTNAAHN-------------DRVGPLVQLEVEGSGFLYRQV 237
           + +M+ AA+YF+G HDFSAF ++  +             +R   +V+ E+ G GFLY  V
Sbjct: 141 FESMQRAASYFLGTHDFSAFRSSGSSAKTSVRTITDVSLEREDKIVKFEIAGDGFLYNMV 200

Query: 238 RNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINY 276
           R +VG L+++G   I  D +P+I+ SR RK   + A   P +GL LV + Y
Sbjct: 201 RIIVGTLMEVGIGKIRADEIPQIIESRKRKRAGRTA---PAEGLYLVEVYY 244

BLAST of Lsi09G015310 vs. ExPASy Swiss-Prot
Match: A5VLH4 (tRNA pseudouridine synthase A OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=truA PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-34
Identity = 86/233 (36.91%), Postives = 129/233 (55.36%), Query Frame = 0

Query: 58  QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVH 117
           Q +  T++  ++ A+    K     + ++G+ RTD GVHA  QVAHF  P++  S E++ 
Sbjct: 21  QPNKRTVEQTLKNAVNKIAKHPTPAIPVIGSGRTDAGVHALNQVAHFDIPYHL-SNESMR 80

Query: 118 AALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLN 177
            ALN +LP+DI +++      DFHAR+S   K Y Y++  G  ++PF+R YT H  Y LN
Sbjct: 81  KALNSILPLDILIKKAELVDNDFHARYSAHRKTYRYRVDQGEFVNPFKRNYTSHFKYPLN 140

Query: 178 YAAMREAANYFVGKHDFSAFTNAA---------------HNDRVGPLVQLEVEGSGFLYR 237
              MR+AA+  VG HDF++F  +                  D V   V  +  G+GFLY 
Sbjct: 141 LEKMRKAADDLVGTHDFTSFVASGSQAKSNVRTIENITIKRDEVRNEVVFDFTGNGFLYN 200

Query: 238 QVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINY 276
           QVR MV  L++IG    P D V ++L ++DR  LA+  +  P  GL LV+++Y
Sbjct: 201 QVRIMVAFLLEIGSNQRPVDDVSRVLKAKDR-TLARMTA--PASGLYLVNVDY 249

BLAST of Lsi09G015310 vs. ExPASy Swiss-Prot
Match: B2G8U7 (tRNA pseudouridine synthase A OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=truA PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.4e-34
Identity = 86/233 (36.91%), Postives = 129/233 (55.36%), Query Frame = 0

Query: 58  QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVH 117
           Q +  T++  ++ A+    K     + ++G+ RTD GVHA  QVAHF  P++  S E++ 
Sbjct: 21  QPNKRTVEQTLKNAVNKIAKHPTPAIPVIGSGRTDAGVHALNQVAHFDIPYHL-SNESMR 80

Query: 118 AALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLN 177
            ALN +LP+DI +++      DFHAR+S   K Y Y++  G  ++PF+R YT H  Y LN
Sbjct: 81  KALNSILPLDILIKKAELVDNDFHARYSAHRKTYRYRVDQGEFVNPFKRNYTSHFKYPLN 140

Query: 178 YAAMREAANYFVGKHDFSAFTNAA---------------HNDRVGPLVQLEVEGSGFLYR 237
              MR+AA+  VG HDF++F  +                  D V   V  +  G+GFLY 
Sbjct: 141 LEKMRKAADDLVGTHDFTSFVASGSQAKSNVRTIENITIKRDEVRNEVVFDFTGNGFLYN 200

Query: 238 QVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINY 276
           QVR MV  L++IG    P D V ++L ++DR  LA+  +  P  GL LV+++Y
Sbjct: 201 QVRIMVAFLLEIGSNQRPVDDVSRVLKAKDR-TLARMTA--PASGLYLVNVDY 249

BLAST of Lsi09G015310 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.0e-145
Identity = 268/329 (81.46%), Postives = 282/329 (85.71%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSPLSPAS-----NGGCSS--LMM 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRGFP+    AS     +  C    L++
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  VPD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120
             D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180
           TPFNYDSLE+VHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV---------------GPLV 240
           RCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRV               G LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCL 300
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

BLAST of Lsi09G015310 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.4e-141
Identity = 262/327 (80.12%), Postives = 276/327 (84.40%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSP------LSPASNGGCSSLMMV 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFP+         P        L++ 
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  PD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
            D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV---------------GPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RV               G LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 299
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

BLAST of Lsi09G015310 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.2e-140
Identity = 260/321 (81.00%), Postives = 272/321 (84.74%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSP------LSPASNGGCSSLMMV 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFP+         P        L++ 
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  PD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
            D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV---------------GPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RV               G LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 293
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

BLAST of Lsi09G015310 vs. ExPASy TrEMBL
Match: A0A1S3C7H4 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.6e-136
Identity = 242/276 (87.68%), Postives = 253/276 (91.67%), Query Frame = 0

Query: 38  SPLSPASNGGCSSLMMVPDTQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHA 97
           SPLSPASNGGCSSLM V D QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHA
Sbjct: 22  SPLSPASNGGCSSLMTVLDMQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHA 81

Query: 98  WGQVAHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYN 157
           WGQVAHF+TPFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYN
Sbjct: 82  WGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYN 141

Query: 158 GAILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV----------- 217
           G I+DPFQRCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RV           
Sbjct: 142 GTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFD 201

Query: 218 ----GPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASG 277
               G LVQLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASG
Sbjct: 202 VIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASG 261

Query: 278 VPPQGLCLVSINYKEDHLKLPSDCPGSSFGCSNNLG 299
           VPPQGLCLVSINYKEDHLKLPSDCPG+S G S ++G
Sbjct: 262 VPPQGLCLVSINYKEDHLKLPSDCPGTSLGRSRSVG 297

BLAST of Lsi09G015310 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.0e-128
Identity = 243/333 (72.97%), Postives = 258/333 (77.48%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSPLSPASNGGCSSLMMVPD---- 60
           M CWAVGS VLP +  PLSPSIP   G  SS+ RGFP       N   SS +  PD    
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIP-SRGTLSSTGRGFP-------NSEDSSCVPEPDFPSF 60

Query: 61  -----------------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 120
                             QRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWG
Sbjct: 61  KWRLVIAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWG 120

Query: 121 QVAHFITPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGA 180
           QVAHFITPFNYDSL+ +HAALNGLLP DIRVREISPA PDFHARFSTLSKVYHYKIYN A
Sbjct: 121 QVAHFITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDA 180

Query: 181 ILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV------------- 240
           ILDPFQRCYTYHCVYKLNYAAM+EAANYFVGKHDFSAF N AHNDRV             
Sbjct: 181 ILDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVI 240

Query: 241 --GPLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVP 298
             G L+QLEVEGSGFLYRQVRNMV LL+QIGKEAIPPDVVPKILASRDRK LAKY + +P
Sbjct: 241 EMGALIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIP 300

BLAST of Lsi09G015310 vs. NCBI nr
Match: XP_038875518.1 (tRNA pseudouridine synthase A 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 531.6 bits (1368), Expect = 4.5e-147
Identity = 267/306 (87.25%), Postives = 278/306 (90.85%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSP------LSPASNGGCSSLMMV 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG P+       ++P        L++ 
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  PD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
            D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVGPLVQLEVEGSGFLYRQVRN 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVGPLVQLEVEGSGFLYRQVRN
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVGPLVQLEVEGSGFLYRQVRN 240

Query: 241 MVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDHLKLPSDC 293
           MVGLLVQIGKEAIPPDVVPKILAS+DR+ELAKYASGVPPQGLCLVSINYKEDHLKLPSDC
Sbjct: 241 MVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLVSINYKEDHLKLPSDC 300

BLAST of Lsi09G015310 vs. NCBI nr
Match: XP_038875517.1 (tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 525.4 bits (1352), Expect = 3.2e-145
Identity = 267/311 (85.85%), Postives = 278/311 (89.39%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSP------LSPASNGGCSSLMMV 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG P+       ++P        L++ 
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  PD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
            D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV-----GPLVQLEVEGSGFLY 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRV     GPLVQLEVEGSGFLY
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKGPLVQLEVEGSGFLY 240

Query: 241 RQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLVSINYKEDHLK 293
           RQVRNMVGLLVQIGKEAIPPDVVPKILAS+DR+ELAKYASGVPPQGLCLVSINYKEDHLK
Sbjct: 241 RQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLVSINYKEDHLK 300

BLAST of Lsi09G015310 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 521.5 bits (1342), Expect = 4.6e-144
Identity = 267/321 (83.18%), Postives = 278/321 (86.60%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSP------LSPASNGGCSSLMMV 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG P+       ++P        L++ 
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  PD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
            D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV---------------GPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRV               GPLVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 293
           LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILAS+DR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

BLAST of Lsi09G015310 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 512.3 bits (1318), Expect = 2.8e-141
Identity = 262/327 (80.12%), Postives = 276/327 (84.40%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSP------LSPASNGGCSSLMMV 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFP+         P        L++ 
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  PD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
            D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180
           PFNYDSLE+VHAALNGLLPVDIRVRE+SPAAPDFHARFSTLSKVYHYKIYNG I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV---------------GPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RV               G LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCLV 299
           LEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRKELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

BLAST of Lsi09G015310 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 511.9 bits (1317), Expect = 3.7e-141
Identity = 262/322 (81.37%), Postives = 276/322 (85.71%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPSPLSPAS-----NGGCSS--LMM 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRGFP+    AS     +  C    L++
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  VPD--------TQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120
             D         QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFNYDSLENVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180
           TPFNYDSLE+VHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRV---------------GPLV 240
           RCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRV               G LV
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYASGVPPQGLCL 293
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

BLAST of Lsi09G015310 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 334.3 bits (856), Expect = 9.8e-92
Identity = 164/251 (65.34%), Postives = 190/251 (75.70%), Query Frame = 0

Query: 58  QRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLENVH 117
           Q SPPTIQ ++E+ALI  T+L RK+L L+GA RTD GVHAWGQVAHF+TPFNY SL++ H
Sbjct: 60  QESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSLDSFH 119

Query: 118 AALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQRCYTYHCVYKLN 177
           AALNGLLP DIRVRE+S A P+FHARFS  SKVY Y+IYN   +DPFQR + YHC YKLN
Sbjct: 120 AALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKLN 179

Query: 178 YAAMREAANYFVGKHDFSAFTNAAHND---------------RVGPLVQLEVEGSGFLYR 237
            + MREAAN FVGKHDFSAF NA   D               ++G L+QLEVEGSGFLYR
Sbjct: 180 ASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSGFLYR 239

Query: 238 QVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKYAS-GVPPQGLCLVSINYKEDHLK 293
           QVRNMV LL+QIGKEA+  D+VP IL ++DR+ LAKY S    P GLCLVS+ YKEDHLK
Sbjct: 240 QVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKEDHLK 299

BLAST of Lsi09G015310 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 73.2 bits (178), Expect = 4.1e-13
Identity = 71/244 (29.10%), Postives = 97/244 (39.75%), Query Frame = 0

Query: 62  PTIQCLIEEALIGATKL--ERKDLHLVGASRTDKGVHAWGQVAHFITPFNY-----DSLE 121
           P+I+  + +AL     L  ++KD       RTDKGV + GQV              DS  
Sbjct: 114 PSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSPPGDSKA 173

Query: 122 NVHA------------ALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILD 181
            V+              LN  LP DIRV   SPA  DFHARFS  ++ Y Y  +      
Sbjct: 174 QVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWR----- 233

Query: 182 PFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAF-------------------TNAAHN 241
                        LN +AM  A   F+G+HDF  F                    +   N
Sbjct: 234 -----------QNLNLSAMDFAGKKFIGEHDFRNFCKMDVANVHCYTRRVTFFEVSPCQN 293

Query: 242 DRVG-PLVQLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRKELAKY--A 265
              G  L    + GS FL+ Q+R MV +L  IG+     DV+  +L ++      +Y  A
Sbjct: 294 SHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVESVDVIDTLLDTKKTPRKPQYLLA 341