Lsi09G014880 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G014880
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	pectinesterase inhibitor-like
Location	chr09: 22937179 .. 22940665 (+)
RNA-Seq Expression	Lsi09G014880
Synteny	Lsi09G014880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCTTCCTTGAGCGAAATGAAGCCTTTATTTCTTGCTCATCAGAATCAGATCTTAGCTGCAAACTTGGAGCCCGAGCGAGCGCTTAACCGCCGTAGAAAGCTTTCAATGCACGGTAAGATTATTTGATAATCATTTGATTTTTACTTATAAATATTACTTTCATGAGTTCTTTTTTGTTTTTTTTTCACATATTTACATTCGTAGGCAAATTCTAAAAAAAAATAAGTTTACAAAAATTATTAATATTATTTTGAAAAACACTTAATTATGAAAGGTAGAAAACAGAATAAAAGTAGTGTTCATAAGCCTAATTTTCAAAAACCAAAAACTAAGGTTTTTGTTTGGTAACTATTTCGTTTTTGGTATTTAGTTTTTTAAAATCAAACCTATTTTCTCACGTTTTCTTACTCTAGTTTGCATATTTCTTGAATACAAGAGTTGAGTTTTTAACCAAATTCCAAAAACAAAAACAAATTTTTAAAAATTACTTTTTTTAGTTTTCAAATTTTGACTTAATTTTTTAAAACATGAATAAAAAGTAGATAAGAAAACAAGATATTTAAGATGAAAGAGATGTTTACATGCTTAATTTTCAAAAACTAAAAATCATAAACTAAATGGTTACAAAACAGACCCAAACAACATCAAACAACGCCTTAAAAACGTAGTTTTTTTTTTTCCGTAATTTGGCTAATAAGTCAAACAAACACATTTAAGAAAAATGAAAAAGAAAATTAAAAGCAAGAATTTAAAAATTAGGAAGATAAATTGGAATGTATAGTACTTCTATGACCAATAATTAAGTATATAACAACATTTTAAAAGAATAAAAAATATAGCAAAATATCACTGATAGACTCCTATCAGCGATATAGTCCATCATGGATAGATTCCGAGAGCTGAATTTAAATTTTTCTATATTTATAAACTCTTTTATATTTTATTATATCTACTAATACTTTGGATCTAATTGCTATATTTGCAATTGTTGAATGATTATAATTATTCGGTATATTTTGTCGAATCAGAAAGATGCAGCAACAGAGACATAAGCATTTCACAGAGCAAAGATTCAACATCAGGAATTCCAGAGTTCATAGTTCAAATTACAAACACTTGTCTCTCACTTGATTGTGCTCCGTCGGATATCCATCTCCATTGCGGCTGGTTTGCCTCTGCAAAAATCGTTAACCCTTCAATTTTCAAGAGAATGTTTTACGATGATTGTTTGGTCAATGGAGGGAAGCCATTGAAGATCAGCCAAACCATTAGATTCACTTACTCTAACTCTTTTATGTATCCTCTCACTTTCAAATCCGCCAACTTTTGTTGACGAGACTAAATTTAAGATTTATTGAAAATACACATGAAACTAAAATTAAACAATTGAATTTATTACGTAGGACCTTCCTCTTTGGCAAATCACTCTCGTCATGCCTCGTATTAGAAATTAAATGTTGATGTTTCTTAGGAGGGTTTTTTTTGGACAAAAAGAGTGTGTGGGTTGGGTAGTGCGTGATTTTGTTAGGTTGTCGATTTGCGTTGGGTGGAAGCAATTCACTTGTAAATGGTGGCTAGTCAAATTGCCTTGGAGGCTCTTGCAATAAAGGAAGAATTGAAGATGTTGTTCTTGAATCTAATGCATTTGAGGTCAAAACTCTTAACCAAAAAGGAGAATAACACATTGTCTCGAAAAACATCTTTCCAGCTGACCATACTTAAAAGAGGCCAAAACTGCGATGTCAAGATCTTGAGAGATGTGACAAAATCCAACAATATTAACAATGTTTTCAAGAAAAAGAATATCTTTAATAAAGACCCCAATTTTAGCCGAAGGAAAGTCCTCTCAATTCAAAAGTTGTTTTTTAATATTTATTTTGAATATTATTATTATTATTATTATTATTATTATTTATTTTTTTAAATTTTTTTTTTGTGAAGGAGAATTAGGGGTGAAGCAAGGTTTTGTAGTGCTAGCAATATGGCTGCTTTTTCAGAAGAGGAAAGAAAAAAGGTTGGTGGCGCAAAGCTGCAAACTTTTGTGTTTTTGTGTGCAATAGGAGCCTACGTATTGGTAAACCCTTATAGCATTTCTCTTTTGTACCGAAAGTGGCTTTTATTTTTTTTTATTTTTATAGTGGAGTATGAAAACTAAATTTAAATAGGATTATTTTCAAATATAGAAAAATAAGATAAATTATTTACAAATATAAAATTTTTTTACTGTCTATCAGCGATAGGCCGCGATAGAATTCAGTCGCTTGAGTGATAGTAATTTGATTTTTTTTTGTTTATAGTAATTTCTCACATAAGCGATAGAATTCTATCGCGGTCTATCGCTGATAACAGTGAAATTTTTATATATTTATAAATAGTTTGATATTTTTTTTGTTTATAATAATTTCTCACACACGAGCGCACACACATACATTGAATTACTATCGCGATCTATCACTAGTAGGCTGTAAAAATTTTCTATATTTGTAAATAGTTTGATATTTTGATGTTTATAGTAATTTCCCATTATTATTTCTAGTCTTCACGAAGGTCTAGCTCCTACTCTGCAATGGGGGTTGAAATGTTGGAACTCAATAAATAAAATTTTTAAAAAAAGACTGCACTGTTTTTTCTGTTAATGTTTTTAAGATAAAAAAAGAAAAACAAAAAACAAAAAACAAACAAAACCCACTACACAAACAGAACTCACATTCCAAAAGGTCAAAATTTCTCTACAAAACTACCTCTCCAACTTCTCCCATCTCCTCCATTAATTTCTTTCATACTTTGAAGTAAAACAAATATAAACAAAAACAAAAATCCTCCAAATTTCAATTATATATATATATATATATATAAATAACCCATTATTTCATTATTTTGAAACAAAGGAAGAATAATAATAATGGGGAATTTGAATCCCAATTCCAGTTTCAATTCAAATCTTGGAGTTGTTAGCTCAATCTTAATTTTCCTTTTTGGTTTATTTTTTTTCAGTGTAATTCTTTTACACGCAGATACAGGCTCTCCAAATGTCATTACAACCACAATTTGCCCAAAAACTAGAAACTCTTCTTTATGTCAAACCGTCTTGAAACCCGCCGGCAAATCTGACACCCTTCTCAAAGTGGCTAATTACACTCTTACCCTTGCCCATACCACCGCCGCCGAAGGCCTCCGTCACGCTCAGCTGCTGGCGGCGGAGGCCACCAATCCATTGCTTAAACAACGCTATTCCGAGTGCTCACGGCGGTTTGATTTTGTGGTGAAGGGTTTAGAAAAGGCCAAAGTGGCGTTGGCTAAAGGTGGGTATATTCCGCTTAGTCATGCCACAGGCGCCGCCGTGGTGGAGGCCGATAGATGTGTAAATATGTTTAAAAAGCCGCCGCCGGAACCGTCGAAGCTGCCGGAGAAGGCTAAGAAATTAGGAGATATTTGTGACATTGCTGTTTCTGTGTCCGCTATTCTCACTGAAGATTACTAA

mRNA sequence

ATGGCGTCTTCCTTGAGCGAAATGAAGCCTTTATTTCTTGCTCATCAGAATCAGATCTTAGCTGCAAACTTGGAGCCCGAGCGAGCGCTTAACCGCCGTAGAAAGCTTTCAATGCACGGAGCCTACGTATTGGTAAACCCTTATAGCATTTCTCTTTTTGTAATTCTTTTACACGCAGATACAGGCTCTCCAAATGTCATTACAACCACAATTTGCCCAAAAACTAGAAACTCTTCTTTATGTCAAACCGTCTTGAAACCCGCCGGCAAATCTGACACCCTTCTCAAAGTGGCTAATTACACTCTTACCCTTGCCCATACCACCGCCGCCGAAGGCCTCCGTCACGCTCAGCTGCTGGCGGCGGAGGCCACCAATCCATTGCTTAAACAACGCTATTCCGAGTGCTCACGGCGGTTTGATTTTGTGGTGAAGGGTTTAGAAAAGGCCAAAGTGGCGTTGGCTAAAGGTGGGTATATTCCGCTTAGTCATGCCACAGGCGCCGCCGTGGTGGAGGCCGATAGATGTGTAAATATGTTTAAAAAGCCGCCGCCGGAACCGTCGAAGCTGCCGGAGAAGGCTAAGAAATTAGGAGATATTTGTGACATTGCTGTTTCTGTGTCCGCTATTCTCACTGAAGATTACTAA

Coding sequence (CDS)

ATGGCGTCTTCCTTGAGCGAAATGAAGCCTTTATTTCTTGCTCATCAGAATCAGATCTTAGCTGCAAACTTGGAGCCCGAGCGAGCGCTTAACCGCCGTAGAAAGCTTTCAATGCACGGAGCCTACGTATTGGTAAACCCTTATAGCATTTCTCTTTTTGTAATTCTTTTACACGCAGATACAGGCTCTCCAAATGTCATTACAACCACAATTTGCCCAAAAACTAGAAACTCTTCTTTATGTCAAACCGTCTTGAAACCCGCCGGCAAATCTGACACCCTTCTCAAAGTGGCTAATTACACTCTTACCCTTGCCCATACCACCGCCGCCGAAGGCCTCCGTCACGCTCAGCTGCTGGCGGCGGAGGCCACCAATCCATTGCTTAAACAACGCTATTCCGAGTGCTCACGGCGGTTTGATTTTGTGGTGAAGGGTTTAGAAAAGGCCAAAGTGGCGTTGGCTAAAGGTGGGTATATTCCGCTTAGTCATGCCACAGGCGCCGCCGTGGTGGAGGCCGATAGATGTGTAAATATGTTTAAAAAGCCGCCGCCGGAACCGTCGAAGCTGCCGGAGAAGGCTAAGAAATTAGGAGATATTTGTGACATTGCTGTTTCTGTGTCCGCTATTCTCACTGAAGATTACTAA

Protein sequence

MASSLSEMKPLFLAHQNQILAANLEPERALNRRRKLSMHGAYVLVNPYSISLFVILLHADTGSPNVITTTICPKTRNSSLCQTVLKPAGKSDTLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAILTEDY

Homology

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BLAST of Lsi09G014880 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 6.7e-15
Identity = 58/175 (33.14%), Postives = 85/175 (48.57%), Query Frame = 0


Query: 49  SISLFVILL-----------HADTGSPNVITTTICPKTRNSSLCQTVLK--PAGKSDTLL 108
           S SLFV LL                + N + + ICPKTRN SLC   L+  P   S  L 
Sbjct: 7   SSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLK 66

Query: 109 KVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAK 168
            +  +++ +A  +A +  +    L  +AT+P LK RY  CS  +   +  L +AK  L  
Sbjct: 67  GLGQFSIDIAQASAKQTSKIIASLTNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTS 126

Query: 169 GGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           G Y  L+    AA   A  C + F+ PP  P++L +   KL D+CDI + +S +L
Sbjct: 127 GDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLL 181

[Close]

BLAST of Lsi09G014880 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.6e-08
Identity = 49/169 (28.99%), Postives = 76/169 (44.97%), Query Frame = 0


Query: 46  NPYSISLFVILLHADTGSPNVIT----TTICPKTRNSSLCQTVLKPAGKSDTLLKVANYT 105
           N +  SL  +LL    GS   IT    +TIC KT N S C   L     S  L  +A  T
Sbjct: 8   NAFLSSLMFLLL---IGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTT 67

Query: 106 LTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGGYIPL 165
           L      A + L+  Q +     +P  K  Y  C   ++  +  LE+A   LA G  + +
Sbjct: 68  LDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGM 127

Query: 166 SHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           +    AA+  AD C++  K+     S +   +K + ++C IA+ +S +L
Sbjct: 128 NMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

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BLAST of Lsi09G014880 vs. ExPASy TrEMBL
Match: A0A0A0KHY0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307370 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 4.0e-71
Identity = 146/194 (75.26%), Postives = 160/194 (82.47%), Query Frame = 0


Query: 23  NLEPERALNRRRKLSMHGAYVLVNPYSISLF-VILLHADTGSPNVITTTICPKTRNSSLC 82
           NL P  + N    +    + +L+  + +  F V+LLHADTGSPNV+TTTICPKTRNS+ C
Sbjct: 3   NLNPNSSFNSNLGVV---SSILIFLFGLFFFSVVLLHADTGSPNVLTTTICPKTRNSTFC 62

Query: 83  QTVLKPAGKSD-TLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFD 142
           +TVLKP GKSD  LLKVANYTLT AHTT  EGL HAQ LA EAT+PLLKQRYSECSRRFD
Sbjct: 63  KTVLKPVGKSDAALLKVANYTLTFAHTTTVEGLHHAQRLATEATDPLLKQRYSECSRRFD 122

Query: 143 FVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDIC 202
           FV KGLE+A  ALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAK +GDIC
Sbjct: 123 FVTKGLEEAIEALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKNIGDIC 182

Query: 203 DIAVSVSAILTEDY 215
           DIAVSVS ILTEDY
Sbjct: 183 DIAVSVSNILTEDY 193

[Close]

BLAST of Lsi09G014880 vs. ExPASy TrEMBL
Match: A0A5D3BT93 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00330 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.8e-66
Identity = 139/197 (70.56%), Postives = 161/197 (81.73%), Query Frame = 0


Query: 20  LAANLEPERALNRRRKLSMHGAYVLVNPYSISLF-VILLHADTGSPNVITTTICPKTRNS 79
           + +NL P  + N    +    + +L+  + +  F V+LLHADTGSPNVIT+TICPKTRNS
Sbjct: 1   MGSNLNPNSSFNSNLGVV---SSILIFLFGLFFFSVVLLHADTGSPNVITSTICPKTRNS 60

Query: 80  SLCQTVLKPAGKSD-TLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSR 139
           +LC+TVLKP GKSD  LLKVANY+LTLAHTT  EGL+HAQ LA +AT+PLLKQRYSECSR
Sbjct: 61  TLCKTVLKPVGKSDAALLKVANYSLTLAHTTTVEGLQHAQRLANDATDPLLKQRYSECSR 120

Query: 140 RFDFVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLG 199
           RFD + KGLE+A  ALAKG YIPLSHATGAAVV+AD CV+MFKK PPEPSKLPEKAK +G
Sbjct: 121 RFDSMTKGLEEAIAALAKGEYIPLSHATGAAVVDADGCVDMFKKRPPEPSKLPEKAKNIG 180

Query: 200 DICDIAVSVSAILTEDY 215
           DICDIAVSVS ILTEDY
Sbjct: 181 DICDIAVSVSNILTEDY 194

[Close]

BLAST of Lsi09G014880 vs. ExPASy TrEMBL
Match: A0A1S3C7Z4 (pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103498015 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.8e-66
Identity = 139/197 (70.56%), Postives = 161/197 (81.73%), Query Frame = 0


Query: 20  LAANLEPERALNRRRKLSMHGAYVLVNPYSISLF-VILLHADTGSPNVITTTICPKTRNS 79
           + +NL P  + N    +    + +L+  + +  F V+LLHADTGSPNVIT+TICPKTRNS
Sbjct: 1   MGSNLNPNSSFNSNLGVV---SSILIFLFGLFFFSVVLLHADTGSPNVITSTICPKTRNS 60

Query: 80  SLCQTVLKPAGKSD-TLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSR 139
           +LC+TVLKP GKSD  LLKVANY+LTLAHTT  EGL+HAQ LA +AT+PLLKQRYSECSR
Sbjct: 61  TLCKTVLKPVGKSDAALLKVANYSLTLAHTTTVEGLQHAQRLANDATDPLLKQRYSECSR 120

Query: 140 RFDFVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLG 199
           RFD + KGLE+A  ALAKG YIPLSHATGAAVV+AD CV+MFKK PPEPSKLPEKAK +G
Sbjct: 121 RFDSMTKGLEEAIAALAKGEYIPLSHATGAAVVDADGCVDMFKKRPPEPSKLPEKAKNIG 180

Query: 200 DICDIAVSVSAILTEDY 215
           DICDIAVSVS ILTEDY
Sbjct: 181 DICDIAVSVSNILTEDY 194

[Close]

BLAST of Lsi09G014880 vs. ExPASy TrEMBL
Match: A0A6J1JS95 (pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111487936 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 2.0e-25
Identity = 74/173 (42.77%), Postives = 98/173 (56.65%), Query Frame = 0


Query: 38  MHGAYVLVNPYSISLFVILLHADTGSPNVITTTICPKTRNSSLCQTVLKPAGKSDTLLKV 97
           M  + VLV      L V+  +    SPN + +TICPKTRN S C  VLK AG SD L  +
Sbjct: 1   MANSIVLVVSSLFVLSVVFSNGYAASPNDVVSTICPKTRNPSFCSNVLKSAGTSD-LKGL 60

Query: 98  ANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGG 157
           A +TL LAH  A +    AQ LA++AT+P LK+ Y  C  RFD  V  +E AK  L  G 
Sbjct: 61  ATFTLNLAHDKATDSRALAQSLASKATDPKLKEHYDTCGERFDDAVDDIEGAKSNLGNGD 120

Query: 158 YIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           Y  ++ +  AA+ EAD C++   KPP + S L +  K + DIC I + VS +L
Sbjct: 121 YNGVNTSASAAMTEADDCLDSLTKPPKDSSALSDNGKTVEDICSIILVVSNLL 172

[Close]

BLAST of Lsi09G014880 vs. ExPASy TrEMBL
Match: A0A6J1GPI8 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111456295 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.8e-24
Identity = 71/173 (41.04%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 38  MHGAYVLVNPYSISLFVILLHADTGSPNVITTTICPKTRNSSLCQTVLKPAGKSDTLLKV 97
           M  + VL       L V+  + D  SPN + +TICPKTRN S C  VLK AG SD L ++
Sbjct: 1   MANSIVLAVSSLFVLSVLFSNGDAASPNDVASTICPKTRNPSFCLDVLKFAGTSD-LKEL 60

Query: 98  ANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGG 157
           A +TL LAH  AAE    AQ LA++A +P LK+RY  C+  +     G+E  K  L +G 
Sbjct: 61  ATFTLNLAHDKAAESHALAQSLASKAADPKLKERYDTCAEFYGNAADGIEDGKNYLGEGD 120

Query: 158 YIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           Y  ++    AA+ EA  C++ F +PP +PS LP   K + DIC I + ++ +L
Sbjct: 121 YNGVNIKASAAMTEAGDCLDSFTQPPKDPSALPGNGKAVEDICSIILVIANLL 172

[Close]

BLAST of Lsi09G014880 vs. NCBI nr
Match: XP_004148232.1 (pectinesterase inhibitor [Cucumis sativus])

HSP 1 Score: 277.7 bits (709), Expect = 8.3e-71
Identity = 146/194 (75.26%), Postives = 160/194 (82.47%), Query Frame = 0


Query: 23  NLEPERALNRRRKLSMHGAYVLVNPYSISLF-VILLHADTGSPNVITTTICPKTRNSSLC 82
           NL P  + N    +    + +L+  + +  F V+LLHADTGSPNV+TTTICPKTRNS+ C
Sbjct: 3   NLNPNSSFNSNLGVV---SSILIFLFGLFFFSVVLLHADTGSPNVLTTTICPKTRNSTFC 62

Query: 83  QTVLKPAGKSD-TLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFD 142
           +TVLKP GKSD  LLKVANYTLT AHTT  EGL HAQ LA EAT+PLLKQRYSECSRRFD
Sbjct: 63  KTVLKPVGKSDAALLKVANYTLTFAHTTTVEGLHHAQRLATEATDPLLKQRYSECSRRFD 122

Query: 143 FVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDIC 202
           FV KGLE+A  ALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAK +GDIC
Sbjct: 123 FVTKGLEEAIEALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKNIGDIC 182

Query: 203 DIAVSVSAILTEDY 215
           DIAVSVS ILTEDY
Sbjct: 183 DIAVSVSNILTEDY 193

[Close]

BLAST of Lsi09G014880 vs. NCBI nr
Match: KAE8647076.1 (hypothetical protein Csa_023043 [Cucumis sativus])

HSP 1 Score: 267.7 bits (683), Expect = 8.6e-68
Identity = 135/156 (86.54%), Postives = 141/156 (90.38%), Query Frame = 0


Query: 60  DTGSPNVITTTICPKTRNSSLCQTVLKPAGKSD-TLLKVANYTLTLAHTTAAEGLRHAQL 119
           DTGSPNV+TTTICPKTRNS+ C+TVLKP GKSD  LLKVANYTLT AHTT  EGL HAQ 
Sbjct: 2   DTGSPNVLTTTICPKTRNSTFCKTVLKPVGKSDAALLKVANYTLTFAHTTTVEGLHHAQR 61

Query: 120 LAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNM 179
           LA EAT+PLLKQRYSECSRRFDFV KGLE+A  ALAKGGYIPLSHATGAAVVEADRCVNM
Sbjct: 62  LATEATDPLLKQRYSECSRRFDFVTKGLEEAIEALAKGGYIPLSHATGAAVVEADRCVNM 121

Query: 180 FKKPPPEPSKLPEKAKKLGDICDIAVSVSAILTEDY 215
           FKKPPPEPSKLPEKAK +GDICDIAVSVS ILTEDY
Sbjct: 122 FKKPPPEPSKLPEKAKNIGDICDIAVSVSNILTEDY 157

[Close]

BLAST of Lsi09G014880 vs. NCBI nr
Match: XP_008458686.1 (PREDICTED: pectinesterase inhibitor-like [Cucumis melo] >TYK02951.1 pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 262.3 bits (669), Expect = 3.6e-66
Identity = 139/197 (70.56%), Postives = 161/197 (81.73%), Query Frame = 0


Query: 20  LAANLEPERALNRRRKLSMHGAYVLVNPYSISLF-VILLHADTGSPNVITTTICPKTRNS 79
           + +NL P  + N    +    + +L+  + +  F V+LLHADTGSPNVIT+TICPKTRNS
Sbjct: 1   MGSNLNPNSSFNSNLGVV---SSILIFLFGLFFFSVVLLHADTGSPNVITSTICPKTRNS 60

Query: 80  SLCQTVLKPAGKSD-TLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSR 139
           +LC+TVLKP GKSD  LLKVANY+LTLAHTT  EGL+HAQ LA +AT+PLLKQRYSECSR
Sbjct: 61  TLCKTVLKPVGKSDAALLKVANYSLTLAHTTTVEGLQHAQRLANDATDPLLKQRYSECSR 120

Query: 140 RFDFVVKGLEKAKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLG 199
           RFD + KGLE+A  ALAKG YIPLSHATGAAVV+AD CV+MFKK PPEPSKLPEKAK +G
Sbjct: 121 RFDSMTKGLEEAIAALAKGEYIPLSHATGAAVVDADGCVDMFKKRPPEPSKLPEKAKNIG 180

Query: 200 DICDIAVSVSAILTEDY 215
           DICDIAVSVS ILTEDY
Sbjct: 181 DICDIAVSVSNILTEDY 194

[Close]

BLAST of Lsi09G014880 vs. NCBI nr
Match: XP_022991215.1 (pectinesterase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 125.9 bits (315), Expect = 4.1e-25
Identity = 74/173 (42.77%), Postives = 98/173 (56.65%), Query Frame = 0


Query: 38  MHGAYVLVNPYSISLFVILLHADTGSPNVITTTICPKTRNSSLCQTVLKPAGKSDTLLKV 97
           M  + VLV      L V+  +    SPN + +TICPKTRN S C  VLK AG SD L  +
Sbjct: 1   MANSIVLVVSSLFVLSVVFSNGYAASPNDVVSTICPKTRNPSFCSNVLKSAGTSD-LKGL 60

Query: 98  ANYTLTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGG 157
           A +TL LAH  A +    AQ LA++AT+P LK+ Y  C  RFD  V  +E AK  L  G 
Sbjct: 61  ATFTLNLAHDKATDSRALAQSLASKATDPKLKEHYDTCGERFDDAVDDIEGAKSNLGNGD 120

Query: 158 YIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           Y  ++ +  AA+ EAD C++   KPP + S L +  K + DIC I + VS +L
Sbjct: 121 YNGVNTSASAAMTEADDCLDSLTKPPKDSSALSDNGKTVEDICSIILVVSNLL 172

[Close]

BLAST of Lsi09G014880 vs. NCBI nr
Match: XP_023549425.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 125.2 bits (313), Expect = 6.9e-25
Identity = 68/159 (42.77%), Postives = 95/159 (59.75%), Query Frame = 0


Query: 52  LFVILLHADTGSPNVITTTICPKTRNSSLCQTVLKPAGKSDTLLKVANYTLTLAHTTAAE 111
           L V+  + D  SPN + +TICPKTRN S C  VLK AG SD L ++A +TL LAH  AAE
Sbjct: 15  LSVLFSNGDAASPNDVVSTICPKTRNPSFCSDVLKSAGTSD-LKELATFTLNLAHDKAAE 74

Query: 112 GLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGGYIPLSHATGAAVVE 171
               AQ LA++A +P L  RY+ C+  +D    G+E  K  L +G Y  ++    AA+ E
Sbjct: 75  SRALAQSLASKAADPKLNDRYATCAELYDNAADGIEDGKKYLGEGDYNGVNIKASAAMTE 134

Query: 172 ADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           A  C++ F +PP +PS LP   K + DIC I + ++ +L
Sbjct: 135 AGDCLDSFTQPPEDPSALPGNGKAVEDICSIILVIANLL 172

[Close]

BLAST of Lsi09G014880 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 60.1 bits (144), Expect = 2.5e-09
Identity = 49/169 (28.99%), Postives = 76/169 (44.97%), Query Frame = 0


Query: 46  NPYSISLFVILLHADTGSPNVIT----TTICPKTRNSSLCQTVLKPAGKSDTLLKVANYT 105
           N +  SL  +LL    GS   IT    +TIC KT N S C   L     S  L  +A  T
Sbjct: 8   NAFLSSLMFLLL---IGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTT 67

Query: 106 LTLAHTTAAEGLRHAQLLAAEATNPLLKQRYSECSRRFDFVVKGLEKAKVALAKGGYIPL 165
           L      A + L+  Q +     +P  K  Y  C   ++  +  LE+A   LA G  + +
Sbjct: 68  LDSTQARATQTLKKLQSIIDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGM 127

Query: 166 SHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVSAIL 211
           +    AA+  AD C++  K+     S +   +K + ++C IA+ +S +L
Sbjct: 128 NMKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Lsi09G014880 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 3.2e-04
Identity = 30/119 (25.21%), Postives = 55/119 (46.22%), Query Frame = 0


Query: 90  KSDTLLKVANYTLTLAHTTAAEGLRHAQLLAAEATNPL-LKQRYSECSRRFDFVVKGLEK 149
           ++++LLK AN TL            +A+ +  +   P+ +K+  S C   +D ++ GLE 
Sbjct: 169 EAESLLKKANATLD-----------YAKRVVEDRKTPMPVKEAMSVCVENYDSLISGLED 228

Query: 150 AKVALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKKLGDICDIAVSVS 208
           AK+A+ +G Y  L     AA+ +   C + F   P   S        +  +C   +++S
Sbjct: 229 AKMAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDSPTASLDDLMKKLCSNVLAMS 276

The following BLAST results are available for this feature:

BLAST of Lsi09G014880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	6.7e-15	33.14	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LNF2	3.6e-08	28.99	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]

BLAST of Lsi09G014880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHY0	4.0e-71	75.26	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307370 PE=4 S...	[more]
A0A5D3BT93	1.8e-66	70.56	Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3C7Z4	1.8e-66	70.56	pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103498015 PE=4 SV=1	[more]
A0A6J1JS95	2.0e-25	42.77	pectinesterase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111487936 PE=4 S...	[more]
A0A6J1GPI8	2.8e-24	41.04	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111456295 PE=4...	[more]

BLAST of Lsi09G014880 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148232.1	8.3e-71	75.26	pectinesterase inhibitor [Cucumis sativus]	[more]
KAE8647076.1	8.6e-68	86.54	hypothetical protein Csa_023043 [Cucumis sativus]	[more]
XP_008458686.1	3.6e-66	70.56	PREDICTED: pectinesterase inhibitor-like [Cucumis melo] >TYK02951.1 pectinestera...	[more]
XP_022991215.1	4.1e-25	42.77	pectinesterase inhibitor-like [Cucurbita maxima]	[more]
XP_023549425.1	6.9e-25	42.77	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi09G014880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48020.1	2.5e-09	28.99	pectin methylesterase inhibitor 1	[more]
AT2G47340.1	3.2e-04	25.21	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 63..206 e-value: 1.0E-9 score: 48.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 52..210 e-value: 2.9E-16 score: 57.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 70..204 e-value: 1.2E-8 score: 35.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 65..210 e-value: 9.2E-20 score: 73.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 70..210
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 37..210
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 37..210
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 70..210 e-value: 6.26707E-36 score: 121.76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G014880.1	Lsi09G014880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity