Lsi09G012960 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G012960
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Chlorophyllase
Location	chr09: 20805162 .. 20808391 (-)
RNA-Seq Expression	Lsi09G012960
Synteny	Lsi09G012960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTACATAAAACTTGAAAGGGCTTATCGCCATACGCGTGAAAGTGTCACTGACATACACAAACGCAAAAAGGGGATGAAAAATTGAAATTGAAACTCCAACAAACCTCACTCGCCACCGCCACCGCCATGGCTATGGCTACTGCTTCTTTTCCTCCGCCGTCGGCCACCGCCGGCTCTGCAATTTCGTCAAATGTCTTCGAGATCGGAAAATTCAGCGCGGTGCTTGAAAAGTTCGAACCGGCTGGATGTTGCTCATCTGGCCGATTTCTTCCGGCTCCCCCGCCGAAGCCGCTGCTGATCGGCCGGCCTTCCGAAGCCGGAGAGTTCCCGGTGCTGCTGCTCATCCATGGCTATCTCCTTTACAACACTTTCTACTCTCAGCTCATCCATCACATCGCCTCTCATGGCTTCATCGTCATCGCCCCTCAGGTTCTCACTTTCTTTTTATTTTTTACTTATTTTTAGATTACTATATCATTTTAGTCTCTACTTTGAATTTTGCTTAATGGTTGAATTTTAGTCTAGCATTTTTAATAAATCTTTTTTTTTAAAACATTATTTCAATTAATCTTAAATTTATTTTCTATTGATAGTTTATGAATGATTTTTTTTAAAAAAAAATTGTTATTTATTAGCATCTTTATAGTAAATTTTAAAAATATATTCATATATTGTATTAAATTATTATGATTATTTAACCAATTTAAGTGAAAAATAACTATAAAGACTAAAATGGTATTTTAATCAATTATTTTTCTTAACTTAAACTATAGGTTTAGTTTTCTTAATAGATTTTACCATGTTGGCTTGTTGGTCAAGTTGCTTAACATGTATGGTGTTATAGAGATTTTGTACGAATGACTCAATTTTTACGCTTAATAAATCATTTGATTTAGGTTTTGAAAACAATTAGGAATAACTTTTTGTTGATGTCACAAACCACTTGCCCAATGCTTTTAAAAAAAAGTGGCAAGCATGAATACAGGCACAAATAAGAGAGTGAGAAAGAGTGCAATGCAAAATAGTAAGGGGTTGAAGAGCCTAATCATTGTGTGAAAACACTATTACATTTAATTAGTTGAGCGCAAGGATAACGAAGAAAGAAAAACATACGAGAGAGAGTGACGCATACAAAATGTTATATATCTTTATTTTCCAAAGCTTGATCAAGCACAGTAAAATTTGGATCATTTGAAAAAGAAAAAAAAAAACTCAACTATATGAGTGCTAATTTAAAATTTTAGATTTATTGTATATATAATTAAAACAAACCATTATAGAAGATTTTTTAGAAAAAAAATAAAATTAAATCATTATAAATGTTGAAATTATAAAAAAAAGTTGTAGAAAATATCATTGTTAAAAGCACTAAAATATCTAATGTGTTAATGCTTGATAATTGTTGATTTTAAGTGGAATCTATAGCTCCACGCCATTGCTTTATTACACAGACTTGTCAAGTGTTGCTTTGTCAACGCAACGAAAATAAAGACATCCCCACTTCTCAAACCCAGTGTGCTTCCACAAAGATCAAAAAGATTTTTTTTCCCTCTCTTTCTTTTTTATGAAACATGTTTCAATCAATTGACTTATCCTTAAATTTGATCATTAAATTATAATTTAAACTTAAATAAATATTATCTAAAAGTATTATATATGTAGGAAAATTTTCACGAATAAAAAAAATGTCAAACTATTTACAGAAAATAACAAAAAAAAACATTGATAAACATGGATAGACTTCTATCAGCATCTATTAGTGATAAACTTTTATCATTTCTATCACGAATAGACTCTGAGAGATTTCTATCGACGTCTATCATAACTGTCTAAAATTTTTGCTATTTTGTATAAATAATATTTAATAATTTTTTTTTATTATTATTATTTTATGAGTTCAATATACAGAAGAGAAAAGTAACTACTAGAATTGAATTTTTAGTAAGATTTGTCCCGTGATTCATCTTGTGCATTAATCAAACTTTAAGTTTATGTTGAACTTGAACAAATAATACAAATTCAAATTTAATTTCAAAGAAACTAATGGCTAAATTTTATATAACTTTATCATCTCTTTCGAAAACAAAAGTTGTTGTAATGGGACACATTAGAATGAAACAAACATTCATTAAAATTAGTCTGGAATGATGTGGAACTTCTATATCTATTTCGTTCTAATTTCTATCCAAATTTTAAAGAATTTTTACTGTAGCTTTAAAATATTTATGAAATGTCACGTTTTAAAAGAATTGTGAATCTTTTAAAATAATGGGCAAAAGTTAATTTTTTTTCTTTTTTGTAATTTAATTTAGGAAAATTTTTAAAACTAGAAAAATAAAATTATTAAAACTTTTAGATAGTTTAATTGACTTTTTTTTTCGTATAGATAGTTTGTCATTTCTTCTCTCGGTTAAAATTTCCCTTTAATTTAGCCCAAATAAACACTCTGTACAGTTGTACTCTGTGGCCGGACCAGATACAAGCGAAGAAATCAAAGCAACAGCCGCCATAATAAACTGGCTGCCGAAAGGCCTCCGGCACCTCCTTCCACCGCACGTGAACCCAAACCTAACCAAAATAGCCCTAGCCGGCCACAGCCGCGGCGGCAAAACTTCCTTCGCCTTAGCCCAACAAAAATCCCCAAAATTGTCAGCCGTAATCGGGCTCGATCCCGTCGACGGAACAGGGTCCGGCAAGCAAACCCATCCCCCAGTTCTGAAATACATCCCCCACTCGTTGGATCTAGGGCTCCCGGTTCTGGTAATCGGGTCGAGTCTGGGAGAATTGAAAAGAAATCCACTGTTTCCCCCTTGCGCCCCAAAAGGAATCAACCACGAGGAGTTCTTTAAGGAGTGCCGGAATCCGGCGTACTATTTTGTTGTGAAGGATTACGGGCATTTGGATTTGTTGGATGATGAAACTGGAGGGATTAGAGGGAAGGTGAGTTACTGTTTGTGTAAAAATGGGGAATCTAGAGAGCCGATGAGGAGGTTTGTTGGTGGGGCTGTGGTTGCGTTTTTGAAGGCTTATTTTGATGGGGAAGAAGGAGATTTGAGAGCCATTGAAGATGGGGATTTGAGCTTGCCTGTTCAACTTCAAACTGTTGAATCTTTTCTCTGAAAATGGCTTACTGCTGAGTAATTTCTTATGGAAAAATGTATGTATCATTTTGGGATTTAAACGGTGGGGGTTGGATTAAGAATACAGTCAAAATTAAGCTTACCTA

mRNA sequence

ATGGGGGATGAAAAATTGAAATTGAAACTCCAACAAACCTCACTCGCCACCGCCACCGCCATGGCTATGGCTACTGCTTCTTTTCCTCCGCCGTCGGCCACCGCCGGCTCTGCAATTTCGTCAAATGTCTTCGAGATCGGAAAATTCAGCGCGGTGCTTGAAAAGTTCGAACCGGCTGGATGTTGCTCATCTGGCCGATTTCTTCCGGCTCCCCCGCCGAAGCCGCTGCTGATCGGCCGGCCTTCCGAAGCCGGAGAGTTCCCGGTGCTGCTGCTCATCCATGGCTATCTCCTTTACAACACTTTCTACTCTCAGCTCATCCATCACATCGCCTCTCATGGCTTCATCTTGTACTCTGTGGCCGGACCAGATACAAGCGAAGAAATCAAAGCAACAGCCGCCATAATAAACTGGCTGCCGAAAGGCCTCCGGCACCTCCTTCCACCGCACGTGAACCCAAACCTAACCAAAATAGCCCTAGCCGGCCACAGCCGCGGCGGCAAAACTTCCTTCGCCTTAGCCCAACAAAAATCCCCAAAATTGTCAGCCGTAATCGGGCTCGATCCCGTCGACGGAACAGGGTCCGGCAAGCAAACCCATCCCCCAGTTCTGAAATACATCCCCCACTCGTTGGATCTAGGGCTCCCGGTTCTGGTAATCGGGTCGAGTCTGGGAGAATTGAAAAGAAATCCACTGTTTCCCCCTTGCGCCCCAAAAGGAATCAACCACGAGGAGTTCTTTAAGGAGTGCCGGAATCCGGCGTACTATTTTGTTGTGAAGGATTACGGGCATTTGGATTTGTTGGATGATGAAACTGGAGGGATTAGAGGGAAGGTGAGTTACTGTTTGTGTAAAAATGGGGAATCTAGAGAGCCGATGAGGAGGTTTGTTGGTGGGGCTGTGGTTGCGTTTTTGAAGGCTTATTTTGATGGGGAAGAAGGAGATTTGAGAGCCATTGAAGATGGGGATTTGAGCTTGCCTGTTCAACTTCAAACTGTTGAATCTTTTCTCTGAAAATGGCTTACTGCTGAGTAATTTCTTATGGAAAAATGTATGTATCATTTTGGGATTTAAACGGTGGGGGTTGGATTAAGAATACAGTCAAAATTAAGCTTACCTA

Coding sequence (CDS)

ATGGGGGATGAAAAATTGAAATTGAAACTCCAACAAACCTCACTCGCCACCGCCACCGCCATGGCTATGGCTACTGCTTCTTTTCCTCCGCCGTCGGCCACCGCCGGCTCTGCAATTTCGTCAAATGTCTTCGAGATCGGAAAATTCAGCGCGGTGCTTGAAAAGTTCGAACCGGCTGGATGTTGCTCATCTGGCCGATTTCTTCCGGCTCCCCCGCCGAAGCCGCTGCTGATCGGCCGGCCTTCCGAAGCCGGAGAGTTCCCGGTGCTGCTGCTCATCCATGGCTATCTCCTTTACAACACTTTCTACTCTCAGCTCATCCATCACATCGCCTCTCATGGCTTCATCTTGTACTCTGTGGCCGGACCAGATACAAGCGAAGAAATCAAAGCAACAGCCGCCATAATAAACTGGCTGCCGAAAGGCCTCCGGCACCTCCTTCCACCGCACGTGAACCCAAACCTAACCAAAATAGCCCTAGCCGGCCACAGCCGCGGCGGCAAAACTTCCTTCGCCTTAGCCCAACAAAAATCCCCAAAATTGTCAGCCGTAATCGGGCTCGATCCCGTCGACGGAACAGGGTCCGGCAAGCAAACCCATCCCCCAGTTCTGAAATACATCCCCCACTCGTTGGATCTAGGGCTCCCGGTTCTGGTAATCGGGTCGAGTCTGGGAGAATTGAAAAGAAATCCACTGTTTCCCCCTTGCGCCCCAAAAGGAATCAACCACGAGGAGTTCTTTAAGGAGTGCCGGAATCCGGCGTACTATTTTGTTGTGAAGGATTACGGGCATTTGGATTTGTTGGATGATGAAACTGGAGGGATTAGAGGGAAGGTGAGTTACTGTTTGTGTAAAAATGGGGAATCTAGAGAGCCGATGAGGAGGTTTGTTGGTGGGGCTGTGGTTGCGTTTTTGAAGGCTTATTTTGATGGGGAAGAAGGAGATTTGAGAGCCATTGAAGATGGGGATTTGAGCTTGCCTGTTCAACTTCAAACTGTTGAATCTTTTCTCTGA

Protein sequence

MGDEKLKLKLQQTSLATATAMAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGRPSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFILYSVAGPDTSEEIKATAAIINWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKLSAVIGLDPVDGTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDGEEGDLRAIEDGDLSLPVQLQTVESFL

Homology

[Close]

BLAST of Lsi09G012960 vs. ExPASy Swiss-Prot
Match: Q9M7I7 (Chlorophyllase-2 OS=Arabidopsis thaliana OX=3702 GN=CLH2 PE=1 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.9e-104
Identity = 187/314 (59.55%), Postives = 236/314 (75.16%), Query Frame = 0


Query: 37  SAISSNVFEIGKFSAVLEKFEPAG-CCS---SGRFLPAPPPKPLLIGRPSEAGEFPVLLL 96
           S+ S N FE GK+ + L   + +  CC    S R  P+ PPK LL+  P E G++PV++L
Sbjct: 3   SSSSRNAFEDGKYKSNLLTLDSSSRCCKITPSSRASPS-PPKQLLVATPVEEGDYPVVML 62

Query: 97  IHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAIINWLPKGLRHLL 156
           +HGYLLYN+FYSQL+ H++SHGFI     LYS+AGPDT +EIK+TA I++WL  GL H L
Sbjct: 63  LHGYLLYNSFYSQLMLHVSSHGFILIAPQLYSIAGPDTMDEIKSTAEIMDWLSVGLNHFL 122

Query: 157 PPHVNPNLTKIALAGHSRGGKTSFALAQQK-----SPKLSAVIGLDPVDGTGSGKQTHPP 216
           P  V PNL+K AL+GHSRGGKT+FA+A +K     + K+S +IG+DPVDGTG GKQT PP
Sbjct: 123 PAQVTPNLSKFALSGHSRGGKTAFAVALKKFGYSSNLKISTLIGIDPVDGTGKGKQTPPP 182

Query: 217 VLKYIPHSLDLG-LPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAYYFVVKD 276
           VL Y+P+S DL   P+LVIGS LGE  RNPLFPPCAP G+NH EFF+EC+ PA++FV KD
Sbjct: 183 VLAYLPNSFDLDKTPILVIGSGLGETARNPLFPPCAPPGVNHREFFRECQGPAWHFVAKD 242

Query: 277 YGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDGEEGDLRAIED 335
           YGHLD+LDD+T GIRGK SYCLCKNGE R PMRRFVGG VV+FLKAY +G++ +L  I+D
Sbjct: 243 YGHLDMLDDDTKGIRGKSSYCLCKNGEERRPMRRFVGGLVVSFLKAYLEGDDRELVKIKD 302

[Close]

BLAST of Lsi09G012960 vs. ExPASy Swiss-Prot
Match: O22527 (Chlorophyllase-1 OS=Arabidopsis thaliana OX=3702 GN=CLH1 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.9e-62
Identity = 131/311 (42.12%), Postives = 180/311 (57.88%), Query Frame = 0


Query: 32  SATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGRPSEAGEFPVLL 91
           S T  S ++   FEIG         +P    S+       PPKP+ I  P+ AG +PV+L
Sbjct: 7   SPTFSSVVTPAAFEIGSLPTTEIPVDPVENDSTA------PPKPVRITCPTVAGTYPVVL 66

Query: 92  LIHGYLLYNTFYSQLIHHIASHGFILYS-----VAGPDTSEEIKATAAIINWLPKGLRHL 151
             HG+ L N FYS +++HIASHG+IL +     +  P    E+    ++INW  + L+  
Sbjct: 67  FFHGFYLRNYFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAH 126

Query: 152 LPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKL------SAVIGLDPVDGTGSGKQTH 211
           LP  VN N    +L GHSRGGKT+FA+A   +  L      SA+IG+DPV GT    +T 
Sbjct: 127 LPTSVNANGKYTSLVGHSRGGKTAFAVALGHAATLDPSITFSALIGIDPVAGTNKYIRTD 186

Query: 212 PPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAYYFVVK 271
           P +L Y P S +L +PV V+G+ LG  K N + PPCAP  +NHEEF+KEC+    +FV  
Sbjct: 187 PHILTYKPESFELDIPVAVVGTGLGP-KWNNVMPPCAPTDLNHEEFYKECKATKAHFVAA 246

Query: 272 DYGHLDLLDDETGGIRGKVSYCLCKNGE-SREPMRRFVGGAVVAFLKAYFDGEEGDLRAI 331
           DYGH+D+LDD+  G  G ++ C+CKNG+  +  MR FVGG VVAFLK    GE+ ++R I
Sbjct: 247 DYGHMDMLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLI 306

[Close]

BLAST of Lsi09G012960 vs. ExPASy Swiss-Prot
Match: Q94LX1 (Chlorophyllase-1, chloroplastic OS=Citrus unshiu OX=55188 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.9e-60
Identity = 126/273 (46.15%), Postives = 168/273 (61.54%), Query Frame = 0


Query: 71  PPPKPLLIGRPSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LY-SVAGPD 130
           PPPKPL+I  P+E G F V+L +HG  L N  YS++  HIASHGFI     LY S+  P 
Sbjct: 48  PPPKPLIIVTPAEKGTFNVILFLHGTSLSNKSYSKIFDHIASHGFIVVAPQLYTSIPPPS 107

Query: 131 TSEEIKATAAIINWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKLSAV 190
            + E+ + A +  WLP+GL+  LP +   N++ +A+ GHSRGG+T+FAL+ +      AV
Sbjct: 108 ATNELNSAAEVAEWLPQGLQQNLPENTEANVSLVAVMGHSRGGQTAFALSLRYG--FGAV 167

Query: 191 IGLDPVDGTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHE 250
           IGLDPV GT       P +L +   S D  +PV VIG+ LG + R      CAP+G NHE
Sbjct: 168 IGLDPVAGTSKTTGLDPSILSF--DSFDFSIPVTVIGTGLGGVAR--CITACAPEGANHE 227

Query: 251 EFFKECRNPA-YYFVVKDYGHLDLLDDETGGIRG-KVSYCLCKNG-ESREPMRRFVGGAV 310
           EFF  C+N +  +FV  DYGH+D+LDD    ++   +S   CKNG ESR+PMRR V G V
Sbjct: 228 EFFNRCKNSSRAHFVATDYGHMDILDDNPSDVKSWALSKYFCKNGNESRDPMRRCVSGIV 287

Query: 311 VAFLKAYFDGEEGDLRAIEDGDLSLPVQLQTVE 335
           VAFLK +FDG+  D R I       P++L +VE
Sbjct: 288 VAFLKDFFDGDAEDFRQILKDPSFAPIKLDSVE 314

[Close]

BLAST of Lsi09G012960 vs. ExPASy Swiss-Prot
Match: Q9MV14 (Chlorophyllase-1, chloroplastic OS=Citrus sinensis OX=2711 GN=CHLASE1 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-58
Identity = 124/273 (45.42%), Postives = 166/273 (60.81%), Query Frame = 0


Query: 71  PPPKPLLIGRPSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LY-SVAGPD 130
           PPPKPL+I  P+  G F V+L +HG  L N  YS++  HIASHGFI     LY S+  P 
Sbjct: 48  PPPKPLIIVTPAGKGTFNVILFLHGTSLSNKSYSKIFDHIASHGFIVVAPQLYTSIPPPS 107

Query: 131 TSEEIKATAAIINWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKLSAV 190
            + E+ + A +  WLP+GL+  LP +   N++ +A+ GHSRGG+T+FAL+ +      AV
Sbjct: 108 ATNELNSAAEVAEWLPQGLQQNLPENTEANVSLVAVMGHSRGGQTAFALSLRYG--FGAV 167

Query: 191 IGLDPVDGTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHE 250
           IGLDPV GT       P +L +   S D  +PV VIG+ LG + R      CAP+G NHE
Sbjct: 168 IGLDPVAGTSKTTGLDPSILSF--DSFDFSIPVTVIGTGLGGVAR--CITACAPEGANHE 227

Query: 251 EFFKECRNPA-YYFVVKDYGHLDLLDDETGGIRG-KVSYCLCKNG-ESREPMRRFVGGAV 310
           EFF  C+N +  +FV  DYGH+D+LDD    ++   +S   CKNG ESR+PMRR V G V
Sbjct: 228 EFFNRCKNSSRAHFVATDYGHMDILDDNPSDVKSWALSKYFCKNGNESRDPMRRCVSGIV 287

Query: 311 VAFLKAYFDGEEGDLRAIEDGDLSLPVQLQTVE 335
           VAFLK +F G+  D R I       P++L +VE
Sbjct: 288 VAFLKDFFYGDAEDFRQILKDPSFAPIKLDSVE 314

[Close]

BLAST of Lsi09G012960 vs. ExPASy Swiss-Prot
Match: Q9LE89 (Chlorophyllase type 0 OS=Chenopodium album OX=3559 GN=CACLH PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.4e-45
Identity = 105/260 (40.38%), Postives = 153/260 (58.85%), Query Frame = 0


Query: 73  PKPLLIGRPSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI--------LYSVAGPD 132
           P+PL+I  P EAG +PVLL IHG +L N  YS   ++IASHGFI        L+    P 
Sbjct: 60  PEPLIIISPKEAGVYPVLLFIHGTMLSNEDYSLFFNYIASHGFIVVAPKLFRLFPPKLPS 119

Query: 133 TSEEIKATAAIINWLPKGLRHLLPPH---VNPNLTKIALAGHSRGGKTSFALAQ-----Q 192
             +EI   A++ NW+P  L+ +L  +   V  +L K+A++GHSRGGK++FALA      +
Sbjct: 120 QQDEIDMAASVANWMPLYLQVVLQRYVTGVEGDLEKLAISGHSRGGKSAFALALGFSNIK 179

Query: 193 KSPKLSAVIGLDPVDGTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPC 252
                SA+IG+DPV G     +T P VL Y P+S +L +PV VIGS LG    +     C
Sbjct: 180 LDVTFSALIGVDPVAGRSVDDRTLPHVLTYKPNSFNLSIPVTVIGSGLGNHTIS-----C 239

Query: 253 APKGINHEEFFKECRNPAYYFVVKDYGHLDLLDD-ETGGIRGKVSYCLCKNGESREPMRR 312
           AP  ++H++F+ EC+  + +FV+  YGH+D+L++     I   +S    ++   +  MRR
Sbjct: 240 APNHVSHQQFYDECKENSSHFVITKYGHMDMLNEFRLSPIAVTMSLMCAQSFRPKATMRR 299

Query: 313 FVGGAVVAFLKAYF--DGEE 314
            +GG +VAFL AYF  DG +
Sbjct: 300 TLGGIMVAFLNAYFRDDGRQ 314

[Close]

BLAST of Lsi09G012960 vs. ExPASy TrEMBL
Match: A0A6J1KYL8 (Chlorophyllase OS=Cucurbita maxima OX=3661 GN=LOC111499382 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.9e-161
Identity = 286/326 (87.73%), Postives = 297/326 (91.10%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPPSATA SA SS VFEIGKF+AVLEK E AGCCSSGR LPAPPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAFSATSSIVFEIGKFNAVLEKVESAGCCSSGRHLPAPPPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS AGEFPVLLLIHGYLLYNTFYSQLIH IASHGFI     LYSVAGPDTSEEIKATAA+
Sbjct: 61  PSIAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSF----ALAQQKSPKLSAVIGLDPVD 200
           INWLP+GLR  LPPHVNPNL KIAL+GHSRGGKTSF    ALA+QKSPKLSA+IGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 201 GTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECR 260
           GTGSGKQTHPPVLKYIP S DLGLPVLVIGS LGELKRNPLFPPCAPKGINHEEFFKECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGLPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFKECR 240

Query: 261 NPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDG 320
           NPA+YFV KDYGHLDLLDDETGG+RGK SYCLCKNG+SREPMRRFVGGAVVAFLKAY +G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGDSREPMRRFVGGAVVAFLKAYLNG 300

Query: 321 EEGDLRAIEDGDLSLPVQLQTVESFL 338
           E+GDLRAIEDGDL LPV LQTVES L
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

[Close]

BLAST of Lsi09G012960 vs. ExPASy TrEMBL
Match: A0A5A7UQQ1 (Chlorophyllase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001310 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.3e-160
Identity = 282/322 (87.58%), Postives = 294/322 (91.30%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPP  TAGSA  SNVFEIGKF+AVLEK EP GCCSSGRFL   PPKPLLIGR
Sbjct: 1   MAMATASFPPPLPTAGSATLSNVFEIGKFNAVLEKVEPGGCCSSGRFL---PPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PSEAGEFPVLLL+HGYLLYNTFYSQLIHHIASHGFI     LY+VAGPD SEEIKATAA+
Sbjct: 61  PSEAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVVAPQLYTVAGPDASEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKLSAVIGLDPVDGTGS 200
           INWLP GLR  LPPHVNPNLTKIALAGHSRGGKTSFALA QKSPKLSA+IGLDPVDGTGS
Sbjct: 121 INWLPTGLRQHLPPHVNPNLTKIALAGHSRGGKTSFALALQKSPKLSALIGLDPVDGTGS 180

Query: 201 GKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAY 260
           GKQTHPPVLKYIP SLDLG+PVLVIGS LGELK NPLFPPCAPKGINHEEFFKECRNPAY
Sbjct: 181 GKQTHPPVLKYIPQSLDLGIPVLVIGSGLGELKMNPLFPPCAPKGINHEEFFKECRNPAY 240

Query: 261 YFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDGEEGD 320
           +FVVKDYGH+DLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLK+  +GEEGD
Sbjct: 241 HFVVKDYGHMDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKSCLNGEEGD 300

Query: 321 LRAIEDGDLSLPVQLQTVESFL 338
           L+AIE GDL LPV LQT+ES L
Sbjct: 301 LKAIEGGDLILPVHLQTIESLL 319

[Close]

BLAST of Lsi09G012960 vs. ExPASy TrEMBL
Match: A0A1S3BME1 (Chlorophyllase OS=Cucumis melo OX=3656 GN=LOC103491233 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.3e-160
Identity = 282/322 (87.58%), Postives = 294/322 (91.30%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPP  TAGSA  SNVFEIGKF+AVLEK EP GCCSSGRFL   PPKPLLIGR
Sbjct: 1   MAMATASFPPPLPTAGSATLSNVFEIGKFNAVLEKVEPGGCCSSGRFL---PPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PSEAGEFPVLLL+HGYLLYNTFYSQLIHHIASHGFI     LY+VAGPD SEEIKATAA+
Sbjct: 61  PSEAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVVAPQLYTVAGPDASEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKLSAVIGLDPVDGTGS 200
           INWLP GLR  LPPHVNPNLTKIALAGHSRGGKTSFALA QKSPKLSA+IGLDPVDGTGS
Sbjct: 121 INWLPTGLRQHLPPHVNPNLTKIALAGHSRGGKTSFALALQKSPKLSALIGLDPVDGTGS 180

Query: 201 GKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAY 260
           GKQTHPPVLKYIP SLDLG+PVLVIGS LGELK NPLFPPCAPKGINHEEFFKECRNPAY
Sbjct: 181 GKQTHPPVLKYIPQSLDLGIPVLVIGSGLGELKMNPLFPPCAPKGINHEEFFKECRNPAY 240

Query: 261 YFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDGEEGD 320
           +FVVKDYGH+DLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLK+  +GEEGD
Sbjct: 241 HFVVKDYGHMDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKSCLNGEEGD 300

Query: 321 LRAIEDGDLSLPVQLQTVESFL 338
           L+AIE GDL LPV LQT+ES L
Sbjct: 301 LKAIEGGDLILPVHLQTIESLL 319

[Close]

BLAST of Lsi09G012960 vs. ExPASy TrEMBL
Match: A0A6J1H5Z8 (Chlorophyllase OS=Cucurbita moschata OX=3662 GN=LOC111459924 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.6e-160
Identity = 282/326 (86.50%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPPSATA SA S  VFEIGKF+AVLEK E AGCCSSGR LP PPPKPLLI R
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIAR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS+AGEFPVLLLIHGYLLYNTFYSQLIH IASHGFI     LYSVAGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSF----ALAQQKSPKLSAVIGLDPVD 200
           INWLP+GLR  LPPHVNPNL KIAL+GHSRGGKTSF    ALA+QKSPKLSA+IGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 201 GTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECR 260
           GTGSGKQTHPPVLKYIP S DLG+PVLVIGS LGELKRNPLFPPCAPKGINHEEFF+ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 261 NPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDG 320
           NPA+YFV KDYGHLDLLDDETGG+RGK SYCLCKNGESREPMRRFVGGAVVAFLKAY +G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 321 EEGDLRAIEDGDLSLPVQLQTVESFL 338
           E+GDLRAIEDGDL LPV LQTVES L
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

[Close]

BLAST of Lsi09G012960 vs. ExPASy TrEMBL
Match: A0A0A0LNT6 (Chlorophyllase OS=Cucumis sativus OX=3659 GN=Csa_2G222090 PE=3 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 4.2e-155
Identity = 276/322 (85.71%), Postives = 290/322 (90.06%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPP AT   AISSNVFEIGKF+AV+EK E  GCCSSGRFL   PPKPLLIGR
Sbjct: 1   MAMATASFPPPLAT---AISSNVFEIGKFNAVIEKVEAGGCCSSGRFL---PPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS+AGEFPVLLL+HGYLLYNTFYSQLIHHIASHGFI     LY+VAGPD SEEI ATAA+
Sbjct: 61  PSDAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVVAPQLYTVAGPDASEEINATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKLSAVIGLDPVDGTGS 200
           +NWLP  LR  LPPHVNPNLTKIALAGHSRGGKTSFALA QKS KLSA+IGLDPVDGTGS
Sbjct: 121 MNWLPTALRQHLPPHVNPNLTKIALAGHSRGGKTSFALALQKSSKLSALIGLDPVDGTGS 180

Query: 201 GKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAY 260
           GKQTHPPVLKYIP S DLG+PVLVIGS LGELKRNPLFPPCAPKGINHEEFFKECR+PAY
Sbjct: 181 GKQTHPPVLKYIPQSFDLGIPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFKECRSPAY 240

Query: 261 YFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDGEEGD 320
           +FVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLK+  +GEEGD
Sbjct: 241 HFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKSRLNGEEGD 300

Query: 321 LRAIEDGDLSLPVQLQTVESFL 338
           L+AIEDGDL LPV LQT ES L
Sbjct: 301 LKAIEDGDLILPVHLQTSESLL 316

[Close]

BLAST of Lsi09G012960 vs. NCBI nr
Match: XP_038906521.1 (chlorophyllase-2 [Benincasa hispida])

HSP 1 Score: 621.3 bits (1601), Expect = 4.8e-174
Identity = 310/341 (90.91%), Postives = 319/341 (93.55%), Query Frame = 0


Query: 2   GDEKLKLKLQQTSLATATAMAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGC 61
           GDEKLKLKL +TSL    AMAMATASF PPS TAGSAISSNVFEIGKF+AVLEK E AGC
Sbjct: 34  GDEKLKLKLPETSL--PIAMAMATASFLPPSTTAGSAISSNVFEIGKFNAVLEKVESAGC 93

Query: 62  CSSGRFLPAPPPKPLLIGRPSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI----- 121
           CSSGRF+PAPPPKPLLIGRPS+AGEFPVLLL+HGYLLYNTFYSQLIHHIASHGFI     
Sbjct: 94  CSSGRFVPAPPPKPLLIGRPSDAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVIAPQ 153

Query: 122 LYSVAGPDTSEEIKATAAIINWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSFALAQQ 181
           LYSVAGPDTSEEIKATAAIINWLP+GLR  LPPHVNPNLTKIALAGHSRGGKTSFALAQQ
Sbjct: 154 LYSVAGPDTSEEIKATAAIINWLPEGLRQHLPPHVNPNLTKIALAGHSRGGKTSFALAQQ 213

Query: 182 KSPKLSAVIGLDPVDGTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPC 241
           KSPKLSA+IGLDPVDGTGSGKQTHPPVLK+IP SLDLGLPVLVIGS LGELKRNPLFPPC
Sbjct: 214 KSPKLSALIGLDPVDGTGSGKQTHPPVLKFIPQSLDLGLPVLVIGSGLGELKRNPLFPPC 273

Query: 242 APKGINHEEFFKECRNPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRF 301
           APKGINHEEFFKECRNPAYYFVVKDYGHLDLLDDETGGIRGK SYCLCKNGESREPMRRF
Sbjct: 274 APKGINHEEFFKECRNPAYYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRF 333

Query: 302 VGGAVVAFLKAYFDGEEGDLRAIEDGDLSLPVQLQTVESFL 338
           VGGAVVAFLKA FDGEEGDL+AIEDGDLSLPV LQTVES L
Sbjct: 334 VGGAVVAFLKACFDGEEGDLKAIEDGDLSLPVLLQTVESLL 372

[Close]

BLAST of Lsi09G012960 vs. NCBI nr
Match: KAG7013529.1 (Chlorophyllase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 578.2 bits (1489), Expect = 4.7e-161
Identity = 284/326 (87.12%), Postives = 297/326 (91.10%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPPSATA SA S  VFEIGKF+AVLEK E AGCCSSGR LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS+AGEFPVLLLIHGYLLYNTFYSQLIH IASHGFI     LYSVAGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSF----ALAQQKSPKLSAVIGLDPVD 200
           INWLP+GLR  LPPHVNPNL KIAL+GHSRGGKTSF    ALA+QKSPKLSA+IGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 201 GTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECR 260
           GTGSGKQTHPPVLKYIPHS DLG+PVLVIGS LGELKRNPLFPPCAPKGINHEEFF+ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPHSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 261 NPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDG 320
           NPA+YFV KDYGHLDLLDDETGG+RGK SYCLCKNGESREPMRRFVGGAVVAFLKAY +G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 321 EEGDLRAIEDGDLSLPVQLQTVESFL 338
           E+GDLRAIEDGDL LPV LQTVES L
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

[Close]

BLAST of Lsi09G012960 vs. NCBI nr
Match: XP_023006741.1 (chlorophyllase-2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 577.4 bits (1487), Expect = 8.0e-161
Identity = 286/326 (87.73%), Postives = 297/326 (91.10%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPPSATA SA SS VFEIGKF+AVLEK E AGCCSSGR LPAPPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAFSATSSIVFEIGKFNAVLEKVESAGCCSSGRHLPAPPPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS AGEFPVLLLIHGYLLYNTFYSQLIH IASHGFI     LYSVAGPDTSEEIKATAA+
Sbjct: 61  PSIAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSF----ALAQQKSPKLSAVIGLDPVD 200
           INWLP+GLR  LPPHVNPNL KIAL+GHSRGGKTSF    ALA+QKSPKLSA+IGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 201 GTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECR 260
           GTGSGKQTHPPVLKYIP S DLGLPVLVIGS LGELKRNPLFPPCAPKGINHEEFFKECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGLPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFKECR 240

Query: 261 NPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDG 320
           NPA+YFV KDYGHLDLLDDETGG+RGK SYCLCKNG+SREPMRRFVGGAVVAFLKAY +G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGDSREPMRRFVGGAVVAFLKAYLNG 300

Query: 321 EEGDLRAIEDGDLSLPVQLQTVESFL 338
           E+GDLRAIEDGDL LPV LQTVES L
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

[Close]

BLAST of Lsi09G012960 vs. NCBI nr
Match: KAG6574961.1 (Chlorophyllase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-160
Identity = 283/326 (86.81%), Postives = 296/326 (90.80%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPPSATA SA S  VFEIGKF+AVLEK E AGCCSSGR LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS+AGEFPVLLLIHGYLLYNTFYSQLIH IASHGFI     LYSVAGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSF----ALAQQKSPKLSAVIGLDPVD 200
           INWLP+GLR  LPPHVNPNL KIAL+GHSRGGKTSF    ALA+QKSPKLSA+IGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 201 GTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECR 260
           GTGSGKQTHPPVLKYIPHS DLG+PVLVIGS LGELKRNPLFPPCAPKGINHEEFF+ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPHSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 261 NPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDG 320
           NPA+YFV KDYGHLDLLDDETGG+RGK SYCLCKNGESREPMRRFVGGAVVAFLKAY +G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 321 EEGDLRAIEDGDLSLPVQLQTVESFL 338
           E+GDLRA EDGDL LPV LQTVES L
Sbjct: 301 EDGDLRAFEDGDLCLPVHLQTVESLL 326

[Close]

BLAST of Lsi09G012960 vs. NCBI nr
Match: XP_023548652.1 (chlorophyllase-2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.9 bits (1483), Expect = 2.3e-160
Identity = 284/326 (87.12%), Postives = 297/326 (91.10%), Query Frame = 0


Query: 21  MAMATASFPPPSATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGR 80
           MAMATASFPPPSATA SA SS VFEIGKF+AVLEK E AGCCSSGR LPAPPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAASATSSIVFEIGKFNAVLEKVESAGCCSSGRHLPAPPPKPLLIGR 60

Query: 81  PSEAGEFPVLLLIHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAI 140
           PS+AGEF VLLLIHGYLLYNTFYSQLIH IASHGFI     LYSVAGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFQVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 141 INWLPKGLRHLLPPHVNPNLTKIALAGHSRGGKTSF----ALAQQKSPKLSAVIGLDPVD 200
           INWLP+GLR  LPPHVNPNL KIAL+GHSRGGKTSF    ALA+QKSPKLSA+IGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 201 GTGSGKQTHPPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECR 260
           GTGSGKQTHPPVLKYIP S DLG+PVLVIGS LGELKRNPLFPPCAPKGINHEEFF+ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 261 NPAYYFVVKDYGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDG 320
           NPA+YFV KDYGHLDLLDDETGG+RGK SYCLCKNGESREPMRRFVGGAVVAFLKAY +G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 321 EEGDLRAIEDGDLSLPVQLQTVESFL 338
           E+GDLRAIEDGDL LPV LQTVES L
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

[Close]

BLAST of Lsi09G012960 vs. TAIR 10
Match: AT5G43860.1 (chlorophyllase 2 )

HSP 1 Score: 380.6 bits (976), Expect = 1.3e-105
Identity = 187/314 (59.55%), Postives = 236/314 (75.16%), Query Frame = 0


Query: 37  SAISSNVFEIGKFSAVLEKFEPAG-CCS---SGRFLPAPPPKPLLIGRPSEAGEFPVLLL 96
           S+ S N FE GK+ + L   + +  CC    S R  P+ PPK LL+  P E G++PV++L
Sbjct: 3   SSSSRNAFEDGKYKSNLLTLDSSSRCCKITPSSRASPS-PPKQLLVATPVEEGDYPVVML 62

Query: 97  IHGYLLYNTFYSQLIHHIASHGFI-----LYSVAGPDTSEEIKATAAIINWLPKGLRHLL 156
           +HGYLLYN+FYSQL+ H++SHGFI     LYS+AGPDT +EIK+TA I++WL  GL H L
Sbjct: 63  LHGYLLYNSFYSQLMLHVSSHGFILIAPQLYSIAGPDTMDEIKSTAEIMDWLSVGLNHFL 122

Query: 157 PPHVNPNLTKIALAGHSRGGKTSFALAQQK-----SPKLSAVIGLDPVDGTGSGKQTHPP 216
           P  V PNL+K AL+GHSRGGKT+FA+A +K     + K+S +IG+DPVDGTG GKQT PP
Sbjct: 123 PAQVTPNLSKFALSGHSRGGKTAFAVALKKFGYSSNLKISTLIGIDPVDGTGKGKQTPPP 182

Query: 217 VLKYIPHSLDLG-LPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAYYFVVKD 276
           VL Y+P+S DL   P+LVIGS LGE  RNPLFPPCAP G+NH EFF+EC+ PA++FV KD
Sbjct: 183 VLAYLPNSFDLDKTPILVIGSGLGETARNPLFPPCAPPGVNHREFFRECQGPAWHFVAKD 242

Query: 277 YGHLDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKAYFDGEEGDLRAIED 335
           YGHLD+LDD+T GIRGK SYCLCKNGE R PMRRFVGG VV+FLKAY +G++ +L  I+D
Sbjct: 243 YGHLDMLDDDTKGIRGKSSYCLCKNGEERRPMRRFVGGLVVSFLKAYLEGDDRELVKIKD 302

[Close]

BLAST of Lsi09G012960 vs. TAIR 10
Match: AT1G19670.1 (chlorophyllase 1 )

HSP 1 Score: 238.8 bits (608), Expect = 6.3e-63
Identity = 131/311 (42.12%), Postives = 180/311 (57.88%), Query Frame = 0


Query: 32  SATAGSAISSNVFEIGKFSAVLEKFEPAGCCSSGRFLPAPPPKPLLIGRPSEAGEFPVLL 91
           S T  S ++   FEIG         +P    S+       PPKP+ I  P+ AG +PV+L
Sbjct: 7   SPTFSSVVTPAAFEIGSLPTTEIPVDPVENDSTA------PPKPVRITCPTVAGTYPVVL 66

Query: 92  LIHGYLLYNTFYSQLIHHIASHGFILYS-----VAGPDTSEEIKATAAIINWLPKGLRHL 151
             HG+ L N FYS +++HIASHG+IL +     +  P    E+    ++INW  + L+  
Sbjct: 67  FFHGFYLRNYFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAH 126

Query: 152 LPPHVNPNLTKIALAGHSRGGKTSFALAQQKSPKL------SAVIGLDPVDGTGSGKQTH 211
           LP  VN N    +L GHSRGGKT+FA+A   +  L      SA+IG+DPV GT    +T 
Sbjct: 127 LPTSVNANGKYTSLVGHSRGGKTAFAVALGHAATLDPSITFSALIGIDPVAGTNKYIRTD 186

Query: 212 PPVLKYIPHSLDLGLPVLVIGSSLGELKRNPLFPPCAPKGINHEEFFKECRNPAYYFVVK 271
           P +L Y P S +L +PV V+G+ LG  K N + PPCAP  +NHEEF+KEC+    +FV  
Sbjct: 187 PHILTYKPESFELDIPVAVVGTGLGP-KWNNVMPPCAPTDLNHEEFYKECKATKAHFVAA 246

Query: 272 DYGHLDLLDDETGGIRGKVSYCLCKNGE-SREPMRRFVGGAVVAFLKAYFDGEEGDLRAI 331
           DYGH+D+LDD+  G  G ++ C+CKNG+  +  MR FVGG VVAFLK    GE+ ++R I
Sbjct: 247 DYGHMDMLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLI 306

The following BLAST results are available for this feature:

BLAST of Lsi09G012960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M7I7	1.9e-104	59.55	Chlorophyllase-2 OS=Arabidopsis thaliana OX=3702 GN=CLH2 PE=1 SV=1	[more]
O22527	8.9e-62	42.12	Chlorophyllase-1 OS=Arabidopsis thaliana OX=3702 GN=CLH1 PE=1 SV=1	[more]
Q94LX1	4.9e-60	46.15	Chlorophyllase-1, chloroplastic OS=Citrus unshiu OX=55188 PE=2 SV=1	[more]
Q9MV14	3.5e-58	45.42	Chlorophyllase-1, chloroplastic OS=Citrus sinensis OX=2711 GN=CHLASE1 PE=1 SV=1	[more]
Q9LE89	4.4e-45	40.38	Chlorophyllase type 0 OS=Chenopodium album OX=3559 GN=CACLH PE=1 SV=1	[more]

BLAST of Lsi09G012960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KYL8	3.9e-161	87.73	Chlorophyllase OS=Cucurbita maxima OX=3661 GN=LOC111499382 PE=3 SV=1	[more]
A0A5A7UQQ1	3.3e-160	87.58	Chlorophyllase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00131...	[more]
A0A1S3BME1	3.3e-160	87.58	Chlorophyllase OS=Cucumis melo OX=3656 GN=LOC103491233 PE=3 SV=1	[more]
A0A6J1H5Z8	9.6e-160	86.50	Chlorophyllase OS=Cucurbita moschata OX=3662 GN=LOC111459924 PE=3 SV=1	[more]
A0A0A0LNT6	4.2e-155	85.71	Chlorophyllase OS=Cucumis sativus OX=3659 GN=Csa_2G222090 PE=3 SV=1	[more]

BLAST of Lsi09G012960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906521.1	4.8e-174	90.91	chlorophyllase-2 [Benincasa hispida]	[more]
KAG7013529.1	4.7e-161	87.12	Chlorophyllase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023006741.1	8.0e-161	87.73	chlorophyllase-2, chloroplastic [Cucurbita maxima]	[more]
KAG6574961.1	1.4e-160	86.81	Chlorophyllase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023548652.1	2.3e-160	87.12	chlorophyllase-2, chloroplastic [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi09G012960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43860.1	1.3e-105	59.55	chlorophyllase 2	[more]
AT1G19670.1	6.3e-63	42.12	chlorophyllase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017395	Chlorophyllase	PFAM	PF07224	Chlorophyllase	coord: 38..330 e-value: 3.9E-110 score: 367.7
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 67..326 e-value: 1.7E-25 score: 91.8
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 78..310
None	No IPR available	PANTHER	PTHR33428:SF13	CHLOROPHYLLASE-2, CHLOROPLASTIC	coord: 35..334
None	No IPR available	PANTHER	PTHR33428	CHLOROPHYLLASE-2, CHLOROPLASTIC	coord: 35..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G012960.1	Lsi09G012960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015996	chlorophyll catabolic process
molecular_function	GO:0047746	chlorophyllase activity
molecular_function	GO:0102293	pheophytinase b activity