Lsi09G012660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G012660
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein SIEVE ELEMENT OCCLUSION B-like
Locationchr09: 20336658 .. 20339231 (-)
RNA-Seq ExpressionLsi09G012660
SyntenyLsi09G012660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGGTATACTTCCTCGAGCAATACAGCTAGAAGAAAACTTAGTTGGTACTTCATACCTATTCTAATTAAGTTACTAACGTGAAAATCATATGTTTCATATATAATATGTTGCAGATGAAATTGATCTCTATAGATGGCTTGTGGATCACATTGATAATTTTCCCACTGAAATAACATTAGTTGTGCCTAAGCTTATTAAAGGCAGGGCTAGTACAAAACCTTTCATTGATGGTTCTACAAGATTGGAGGTCCTTGCCATTCATACTTAATTTGTTTTTTATATATTATTGATTTATTAAATCTATTTAATATGTATATGCCTATACAGGTTAGTGTTGAAGATGCTTTGGAAGGCAAGAATGTGATCTTGATAATTTCTCGATTAGATATATCTGAGGATGATATTAGAGCTCTTCATTCAATTTACAATGAAGTGAAAAAGGGAGATAAATACAAGATGGTTTGGATCCCTGTAATTACTGTAGAATCTCAAGAAGAAGAAGAAGAAGAAGTAGCTAAAAAGAAGTATGAATATTTAAGCTCTTTAATGAAATGGTATATTGTGCCATACACTACAGAAATTGCAGGTTTGAGATATCTTGAGGAGAATTGGCAACTTAGACAAGATCCATTGGTTATTGTTATAGATTCAACATCAGGAGTTAAATTCACAAATGCAATCCATTTGATTCGAGTTTGGGGAACTGAAGCTATTCCATTCACCCAGGGAAGAACTAATGAATTGTTGAGAAAGAATTGGCCTGAATCCACCCTTTTTAAATTTACTAAGCAACCAAGATTAAACAGTTGGGTAAGTGGTTTTATCTTTCCTTTTTCATTCCCACATATTTATGCTTTTTAAATGTTTTTTTCCATGAATTTTGAGTATAGTTTTGTTTGGTTTTTAGGTTTTAAAATGTTATCTTTTGAGCTTAGTTTTCATTTGGTTTCTAGATTTCTAGATTTTACACTTTTGCCTTTGAGTTTTTACTAATATTTATTTTTTTTTATTTGATTTTAGTAGTGATGGAAAATTTAATGAAAACTGATTTAATTTTAGTTAATATAATTCTTTTAAATTAATTATTACAATATTTAACATAAATGACTAAAAAGTGAGCATTAATAAAAAAAAAAAATCAGAGATAAAATATAAAATGTTAAAATGTAGTCTAAATGGAAATTTATAAACTCACAATTCGAAGGTAAAAATACAACCTTTTGAAATTAAAGGTGCAAATTAAAACTAAACTATTTACTAGGCGTAAAAGTGCAACACTTTTTGAAACCTGACCAAACAAAAATAATTAAATTCAAGAACTAAAAATGTATTTTTTTCCTTTAAACTTACGATAACCCATTACCTTTGTCTCCATTTAGTATTGAAATCGTTTTTAATTTTCTATTTTTAAAATTTATGTTTGTTTCTTCTCAAATTTTCCAGTTATGATTTTCATGTTTTTGAAAGAATCAATTAATCCCTAGAGAAATTCTAAAAACAAAAACAAGTTTTTTGAAAACATACTTTTTTTTTAGTTTTCTAAAGTTGATTTGTTGTTGAATAAAGCATTGATAGAAAAAATTGATAACAAAACATAGAAACTTATGAACGGAATTAGTATTTATAAGCTTAACTTTCAAAAATCAAATGGTTATCAAACTGGAGACCTTAACTATTTGAGTTCACTTAATAGTGATTTAATATGAATATCATAGCAATGTTCCATCGGTTTAATTAAGCTTTTATATTCAGTAGTGATTTAACATGCATTGATGAAACATCTTCTATTTCGTTTTTGTTAGGTCAATCAAGAGAGAAACATCATATTTTATGGTGGAAAAGATCCAAATTGGATCCAACAATTCGAAAAGAAAGTAGTAGAGATTAAGAATGACCCCTACATAAAGAAAAAAGGAAACACATTCGAAATCATACGAGTGGGACAAAACATCAAAGGAGAGAACAATTATTTTACACTTACACCTCATTTTTGGATAACCCAATGGAGTTACTTCGTGATCAAGAGTCAATTACGAGGTTCAAATGCAACTGAAACAACTGATGATATCCTACGACTCATATCGTACGAAAACGAAAATGGTTGGGCAATCTTAGCCGTTGGATTAACTCCATTGCTTGTTGGGCGTGGCAATTTGATTTTGGGAGTTCTCCAAGATTTCAACGAATGGAAAAGAGATATAAACATAAAAACTTTCTCTAAGGCATTTATAGATTACTTCACTGAGTTGAATCTCAAATTTCATACTTGTGACAGAATGACACTTCCTGGATTTAGTGGATGGATTCCTATGATTATAAATTGTCCTGAATGTCCTCGCTTCATGGAGGCTGGCATAAGCTTCAAGTGTAACCATGGGCGCCCTGAATTGACATGAAGATGATGACGATATCAAAGACACAAATGTTAGGTATATAATATATACTATATATATTATATATGTACTACTCTACTACTAAATAATATAAGGTTCCTCTGACTACATCTATTTTACTATTTCATCAAGTTTGAGCCTATTAGGCTACAATGAATTATTCCATATTGATGATA

mRNA sequence

ATGAAAGATGAAATTGATCTCTATAGATGGCTTGTGGATCACATTGATAATTTTCCCACTGAAATAACATTAGTTGTGCCTAAGCTTATTAAAGGCAGGGCTAGTACAAAACCTTTCATTGATGGTTCTACAAGATTGGAGGTTAGTGTTGAAGATGCTTTGGAAGGCAAGAATGTGATCTTGATAATTTCTCGATTAGATATATCTGAGGATGATATTAGAGCTCTTCATTCAATTTACAATGAAGTGAAAAAGGGAGATAAATACAAGATGGTTTGGATCCCTGTAATTACTGTAGAATCTCAAGAAGAAGAAGAAGAAGAAGTAGCTAAAAAGAAGTATGAATATTTAAGCTCTTTAATGAAATGGTATATTGTGCCATACACTACAGAAATTGCAGGTTTGAGATATCTTGAGGAGAATTGGCAACTTAGACAAGATCCATTGGTTATTGTTATAGATTCAACATCAGGAGTTAAATTCACAAATGCAATCCATTTGATTCGAGTTTGGGGAACTGAAGCTATTCCATTCACCCAGGGAAGAACTAATGAATTGTTGAGAAAGAATTGGCCTGAATCCACCCTTTTTAAATTTACTAAGCAACCAAGATTAAACAGTTGGGTCAATCAAGAGAGAAACATCATATTTTATGGTGGAAAAGATCCAAATTGGATCCAACAATTCGAAAAGAAAGTAGTAGAGATTAAGAATGACCCCTACATAAAGAAAAAAGGAAACACATTCGAAATCATACGAGTGGGACAAAACATCAAAGGAGAGAACAATTATTTTACACTTACACCTCATTTTTGGATAACCCAATGGAGTTACTTCGTGATCAAGAGTCAATTACGAGGTTCAAATGCAACTGAAACAACTGATGATATCCTACGACTCATATCGTACGAAAACGAAAATGGTTGGGCAATCTTAGCCGTTGGATTAACTCCATTGCTTGTTGGGCGTGGCAATTTGATTTTGGGAGTTCTCCAAGATTTCAACGAATGGAAAAGAGATATAAACATAAAAACTTTCTCTAAGGCATTTATAGATTACTTCACTGAGTTGAATCTCAAATTTCATACTTGTGACAGAATGACACTTCCTGGATTTAGTGGATGGATTCCTATGATTATAAATTGTCCTGAATGTCCTCGCTTCATGGAGGCTGGCATAAGCTTCAAGTGTAACCATGGGCGCCCTGAATTGACATGAAGATGATGACGATATCAAAGACACAAATGTTAGGTATATAATATATACTATATATATTATATATGTACTACTCTACTACTAAATAATATAAGGTTCCTCTGACTACATCTATTTTACTATTTCATCAAGTTTGAGCCTATTAGGCTACAATGAATTATTCCATATTGATGATA

Coding sequence (CDS)

ATGAAAGATGAAATTGATCTCTATAGATGGCTTGTGGATCACATTGATAATTTTCCCACTGAAATAACATTAGTTGTGCCTAAGCTTATTAAAGGCAGGGCTAGTACAAAACCTTTCATTGATGGTTCTACAAGATTGGAGGTTAGTGTTGAAGATGCTTTGGAAGGCAAGAATGTGATCTTGATAATTTCTCGATTAGATATATCTGAGGATGATATTAGAGCTCTTCATTCAATTTACAATGAAGTGAAAAAGGGAGATAAATACAAGATGGTTTGGATCCCTGTAATTACTGTAGAATCTCAAGAAGAAGAAGAAGAAGAAGTAGCTAAAAAGAAGTATGAATATTTAAGCTCTTTAATGAAATGGTATATTGTGCCATACACTACAGAAATTGCAGGTTTGAGATATCTTGAGGAGAATTGGCAACTTAGACAAGATCCATTGGTTATTGTTATAGATTCAACATCAGGAGTTAAATTCACAAATGCAATCCATTTGATTCGAGTTTGGGGAACTGAAGCTATTCCATTCACCCAGGGAAGAACTAATGAATTGTTGAGAAAGAATTGGCCTGAATCCACCCTTTTTAAATTTACTAAGCAACCAAGATTAAACAGTTGGGTCAATCAAGAGAGAAACATCATATTTTATGGTGGAAAAGATCCAAATTGGATCCAACAATTCGAAAAGAAAGTAGTAGAGATTAAGAATGACCCCTACATAAAGAAAAAAGGAAACACATTCGAAATCATACGAGTGGGACAAAACATCAAAGGAGAGAACAATTATTTTACACTTACACCTCATTTTTGGATAACCCAATGGAGTTACTTCGTGATCAAGAGTCAATTACGAGGTTCAAATGCAACTGAAACAACTGATGATATCCTACGACTCATATCGTACGAAAACGAAAATGGTTGGGCAATCTTAGCCGTTGGATTAACTCCATTGCTTGTTGGGCGTGGCAATTTGATTTTGGGAGTTCTCCAAGATTTCAACGAATGGAAAAGAGATATAAACATAAAAACTTTCTCTAAGGCATTTATAGATTACTTCACTGAGTTGAATCTCAAATTTCATACTTGTGACAGAATGACACTTCCTGGATTTAGTGGATGGATTCCTATGATTATAAATTGTCCTGAATGTCCTCGCTTCATGGAGGCTGGCATAAGCTTCAAGTGTAACCATGGGCGCCCTGAATTGACATGA

Protein sequence

MKDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVILIISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLMKWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQGRTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPYIKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLISYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKFHTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT
Homology
BLAST of Lsi09G012660 vs. ExPASy Swiss-Prot
Match: Q9SS87 (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.8e-40
Identity = 122/422 (28.91%), Postives = 191/422 (45.26%), Query Frame = 0

Query: 14  HIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVILIISRLDISEDDI 73
           HIDN      LV PK         P  DG T+ +V + D L  K V+L+IS L+I +D++
Sbjct: 320 HIDNMKILTALVHPK-----PHITPLQDGLTKRKVHL-DVLRRKTVLLLISDLNILQDEL 379

Query: 74  RALHSIYNEVKKG---------DKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLMKWY 133
                IY E ++            Y++VW+PV+    ++ E   + +KK+E L   M WY
Sbjct: 380 SIFEQIYTESRRNLVGVDGKSHMPYEVVWVPVVD-PIEDFERSPILQKKFEDLRDPMPWY 439

Query: 134 IV--PYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQGR 193
            V  P   E   + ++   W     P+++VID        NA+H+I +WGTEA PFT+ R
Sbjct: 440 SVDSPKLIERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSR 499

Query: 194 TNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKND--- 253
             EL R+      L        + +W+  +  I  YGG D +WI++F         D   
Sbjct: 500 EEELWRRETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNV 559

Query: 254 ----PYIKKKGNTF--EIIRVGQNIKGENNYFT-----LTPHFWITQWSYFVIKSQL-RG 313
                Y+ K+ ++   +I R+ + I+ EN   +     L   FW    S    K QL + 
Sbjct: 560 NLEMAYVGKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKA 619

Query: 314 SNATETTDDILRLISYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFS 373
            +  +    I +++SY+   GWA+L+ G   +++  G +   +      WK  +  K ++
Sbjct: 620 DDHDDVMQGIKKILSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYT 679

Query: 374 KAFIDYFTELNLK----------FHTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKC 400
           KA  D+  +  L+          FH   R      SG IP  +NC EC R ME  +SF C
Sbjct: 680 KAMSDHHHDEVLRETGKPCGHFDFHITAR------SGRIPEKMNCFECQRPMEKYMSFSC 728

BLAST of Lsi09G012660 vs. ExPASy Swiss-Prot
Match: Q93XX2 (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-30
Identity = 101/374 (27.01%), Postives = 171/374 (45.72%), Query Frame = 0

Query: 52  DALEGKNVILIISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAK 111
           + L  K+V+L+IS L+  E ++  L S+Y E  +   ++++W+PV      ++   E   
Sbjct: 455 NVLTQKHVLLLISDLENIEKELYILESLYTEAWQ-QSFEILWVPV------QDFWTEADD 514

Query: 112 KKYEYLSSLMKWYIV--PYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIR 171
            K+E L   M+WY++  P     A +R++ E W  +  P+++ +D    V  TNA  ++ 
Sbjct: 515 AKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVW 574

Query: 172 VWGTEAIPFTQGRTNEL-LRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQ 231
           +W   A PFT  R  +L   + W    L   T    LN  V+  + I  YGG+D  WI+ 
Sbjct: 575 IWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVD-GKYICLYGGEDMQWIKN 634

Query: 232 FEK------KVVEIKND-PYIKKKGNTFEIIRVGQNIKGENNYFTLTP-----HFWITQW 291
           F        K   I+ +  Y+ K+     I  +   I+ EN   TL        FW    
Sbjct: 635 FTSLWRNVAKAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVE 694

Query: 292 SYFVIKSQLRGSNATE------------TTDDILRLISYENE-NGWAILAVGLTPLLVGR 351
           S +  K ++  ++  +               +++ ++ Y  E +GW +++     ++  +
Sbjct: 695 SMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAK 754

Query: 352 GNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKFHTCDRMTLPGFSGWIPMIINCP 398
           GNL    L +FNEW+ +I  K F  A  D+   + L  H C R  LP  +G IP  + C 
Sbjct: 755 GNLFSRGLAEFNEWEVNIPTKGFLTALNDHLL-MRLPPHHCTRFMLPETAGIIPNEVECT 814

BLAST of Lsi09G012660 vs. ExPASy Swiss-Prot
Match: Q9FXE2 (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 1.5e-10
Identity = 62/292 (21.23%), Postives = 130/292 (44.52%), Query Frame = 0

Query: 47  EVSVEDALEGKNVILIISRLDISEDDIRALHSIY---NEVKKGDKYKMVWIPVITVESQE 106
           ++S+ + ++ K  +L++S+  + E     L  +Y   +       Y+++W+P+ + +   
Sbjct: 317 QISITE-VQDKVTLLLLSKPPV-EPLFFLLQQLYDHPSNTNTEQNYEIIWVPIPSSQKWT 376

Query: 107 EEEEEVAKKKYEYLSSLMKWYIV--PYTTEIAGLRYLEENWQLR-QDPLVIVIDSTSGVK 166
           +EE+E+    +++ S+ + W  V  P+      L + ++ W  +  + +++VIDS     
Sbjct: 377 DEEKEI----FDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDNEAMLVVIDSNGRFV 436

Query: 167 FTNAIHLIRVWGTEAIPFTQGRTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGG 226
             NA+ ++ +WG +A PF+  R +EL +++     L      P       + R I  +G 
Sbjct: 437 NMNAMDMVLIWGVKAYPFSVSREDELWKEHGWSINLLLDGIHPTF-----EGREICIFGS 496

Query: 227 KDPNWIQQFEKKVVEIKNDPYIKKKGNTFEIIRVGQNIKGENNYF--------TLTPHFW 286
           ++ +WI +F     +I+N       G   E+I +    + E            TL   FW
Sbjct: 497 ENLDWIDEFVSLARKIQN------LGFQLELIYLSNQRRDERAMEESSILFSPTLQQLFW 556

Query: 287 ITQWSYFVIKSQLR-----GSNATETTDDILRLI--SYENENGWAILAVGLT 318
           +   S  + +S+L+      S      +++  L+   Y    GW I+  G T
Sbjct: 557 LRLES--IERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGWGIIGNGST 589

BLAST of Lsi09G012660 vs. ExPASy Swiss-Prot
Match: Q7Y0E8 (Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405500 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 1.6e-04
Identity = 32/109 (29.36%), Postives = 62/109 (56.88%), Query Frame = 0

Query: 80  YNE-VKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLMKWYIVPYTTEIAGLRYL 139
           YNE V +G  +++V++      S ++++E      ++   + M W  VP++      + L
Sbjct: 60  YNELVSQGKNFEVVFV------SGDKDQE-----AFDAYFAKMPWLAVPFSDSECRAK-L 119

Query: 140 EENWQLRQDPLVIVIDSTSGVKFT-NAIHLIRVWGTEAIPFTQGRTNEL 187
            + +++R  P ++++++TSG  +T + + L+ V GTEA PFT  R NEL
Sbjct: 120 NKRFKVRGIPHLVILNATSGEVYTEDGVELVTVHGTEAYPFTTERINEL 156

BLAST of Lsi09G012660 vs. ExPASy Swiss-Prot
Match: Q7Y0F2 (Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405900 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 5.9e-04
Identity = 32/109 (29.36%), Postives = 59/109 (54.13%), Query Frame = 0

Query: 80  YNE-VKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLMKWYIVPYTTEIAGLRYL 139
           YNE V +G  +++V++      S + +++      +    + M W  VP++   A L  L
Sbjct: 67  YNELVSQGKSFEVVFV------SGDSDQD-----AFNAYFAKMPWLAVPFSDSEA-LAKL 126

Query: 140 EENWQLRQDPLVIVIDSTSGVKFT-NAIHLIRVWGTEAIPFTQGRTNEL 187
            E +++   P ++++D+ SG  +T + + L+  +GTEA PFT  R NEL
Sbjct: 127 NERYKVMGIPHLVILDAKSGEIYTEDGVELVHEYGTEAYPFTTERINEL 163

BLAST of Lsi09G012660 vs. ExPASy TrEMBL
Match: A0A0A0LNQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G193350 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.1e-197
Identity = 326/404 (80.69%), Postives = 365/404 (90.35%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           +DEIDLYRWLVDHIDNFPTEIT VVPKLI+G+   KPFIDGST+L+VSVED L  KNVIL
Sbjct: 289 QDEIDLYRWLVDHIDNFPTEITAVVPKLIEGKFDAKPFIDGSTKLQVSVEDGLRDKNVIL 348

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           +IS LDISEDDIRALHSIYNEVK+ DKYK+VWIPVITVE+++EEEE  A+KKYEY SSLM
Sbjct: 349 VISGLDISEDDIRALHSIYNEVKREDKYKIVWIPVITVETEDEEEE--ARKKYEYASSLM 408

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYT +IAG RYLEENWQLRQDPL++V++S S V+F NAIHLIRVWG +AIPFT G
Sbjct: 409 KWYIVPYTRKIAGWRYLEENWQLRQDPLIVVMNSKSRVEFNNAIHLIRVWGIDAIPFTNG 468

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RTN LL KNWPESTLFKF  QPRL +WVNQERNIIFYGGK+P WIQQFE ++VEIKNDPY
Sbjct: 469 RTNALLAKNWPESTLFKFIDQPRLMNWVNQERNIIFYGGKEPKWIQQFEDRIVEIKNDPY 528

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           +K+KGNTFEIIRVGQNIKG++N FTLTP FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 529 LKEKGNTFEIIRVGQNIKGDSNDFTLTPQFWLTQWGYFVIKSQLKGSSATETTEDILRLI 588

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAI+AVG TPLLVGRGNLI+GVLQDFN+WKR++NIK F  AF DYF ELNL F
Sbjct: 589 SYENENGWAIVAVGSTPLLVGRGNLIMGVLQDFNKWKRNMNIKAFPDAFRDYFNELNLNF 648

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           H C+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGR EL+
Sbjct: 649 HICERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRYELS 690

BLAST of Lsi09G012660 vs. ExPASy TrEMBL
Match: A0A6J1KK25 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111494729 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.2e-194
Identity = 326/405 (80.49%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EIDLYRWLVDHIDNFPTEITLV+PKL++G+   KPFIDGSTRL+VSVED L  KNVIL
Sbjct: 290 QEEIDLYRWLVDHIDNFPTEITLVLPKLVEGKVEAKPFIDGSTRLQVSVEDGLRDKNVIL 349

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQE--EEEEEVAKKKYEYLSS 121
           +IS LDISEDDIRALH +YNEV+K DKYK+VWIPVI   S E  +EEEE A+KKYEYLSS
Sbjct: 350 LISGLDISEDDIRALHLVYNEVRKEDKYKIVWIPVIHEHSPEHSQEEEEEARKKYEYLSS 409

Query: 122 LMKWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFT 181
           LMKWYIVPYTT+IAG RYLEENWQLRQDPLV+V++S S V+FTNAIHLIRVWGTEAIPFT
Sbjct: 410 LMKWYIVPYTTKIAGWRYLEENWQLRQDPLVVVMNSQSRVEFTNAIHLIRVWGTEAIPFT 469

Query: 182 QGRTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKND 241
            GRT+ LL KNWPESTL KF +QPRL SWVNQ+R IIFYGGKDPNWIQQFE+KVVEIKND
Sbjct: 470 NGRTHALLEKNWPESTLLKFIEQPRLRSWVNQDRAIIFYGGKDPNWIQQFEEKVVEIKND 529

Query: 242 PYIKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILR 301
           PYIK KGNTFEI+RVG+ I  E N F LTP FWITQW YFVIKSQL+GS+A ETT+DILR
Sbjct: 530 PYIKDKGNTFEIVRVGKKI-NEVNDFALTPLFWITQWGYFVIKSQLKGSSAAETTEDILR 589

Query: 302 LISYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNL 361
           LISYENENGWA+LAVG  PLLVGRGNLILGVL+DFN+WKR++NI+ F  AF DYF ELNL
Sbjct: 590 LISYENENGWAVLAVGSAPLLVGRGNLILGVLEDFNKWKRNLNIEAFPDAFKDYFNELNL 649

Query: 362 KFHTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPEL 405
           KFHTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL
Sbjct: 650 KFHTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPEL 693

BLAST of Lsi09G012660 vs. ExPASy TrEMBL
Match: A0A5A7UNT2 (Protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00080 PE=4 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 2.1e-193
Identity = 318/404 (78.71%), Postives = 364/404 (90.10%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EI+LY+WLVDHIDNFPTEITLVVPKLI+G+   KPFIDGST+L+VS+ED L  KNVIL
Sbjct: 272 REEIELYKWLVDHIDNFPTEITLVVPKLIEGKFDAKPFIDGSTKLQVSIEDGLRDKNVIL 331

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           +IS LDISEDDIRALHSIY+EVKK DKYK+VWIPVITVE+Q+EEE+  A+KKYEY+SSLM
Sbjct: 332 VISGLDISEDDIRALHSIYDEVKKEDKYKIVWIPVITVETQDEEEK--ARKKYEYVSSLM 391

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYT++IAG RYLEENWQLRQDPLV+V++S S V+F NAIHLIRVWG +A PFT G
Sbjct: 392 KWYIVPYTSKIAGWRYLEENWQLRQDPLVVVMNSKSRVEFNNAIHLIRVWGIDAFPFTNG 451

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RTN LL KNWPESTLFKF  QPRL +WVNQ+RNIIFYGGKDPNWIQQFE+++ EIKNDPY
Sbjct: 452 RTNALLAKNWPESTLFKFIDQPRLMNWVNQDRNIIFYGGKDPNWIQQFEERIEEIKNDPY 511

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           IK++ NTFEIIRVGQ +K ++N  TLT  FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 512 IKERRNTFEIIRVGQKLKEDSNDITLTARFWLTQWGYFVIKSQLKGSSATETTEDILRLI 571

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAI+AVG  PLLVGRGNLI+GVLQDFN+WKR +N+K F  AF DYF ELNLKF
Sbjct: 572 SYENENGWAIVAVGSAPLLVGRGNLIMGVLQDFNKWKRSMNMKIFPDAFRDYFNELNLKF 631

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           HTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL+
Sbjct: 632 HTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPELS 673

BLAST of Lsi09G012660 vs. ExPASy TrEMBL
Match: A0A1S3CN79 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo OX=3656 GN=LOC103502853 PE=4 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 2.1e-193
Identity = 318/404 (78.71%), Postives = 364/404 (90.10%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EI+LY+WLVDHIDNFPTEITLVVPKLI+G+   KPFIDGST+L+VS+ED L  KNVIL
Sbjct: 275 REEIELYKWLVDHIDNFPTEITLVVPKLIEGKFDAKPFIDGSTKLQVSIEDGLRDKNVIL 334

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           +IS LDISEDDIRALHSIY+EVKK DKYK+VWIPVITVE+Q+EEE+  A+KKYEY+SSLM
Sbjct: 335 VISGLDISEDDIRALHSIYDEVKKEDKYKIVWIPVITVETQDEEEK--ARKKYEYVSSLM 394

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYT++IAG RYLEENWQLRQDPLV+V++S S V+F NAIHLIRVWG +A PFT G
Sbjct: 395 KWYIVPYTSKIAGWRYLEENWQLRQDPLVVVMNSKSRVEFNNAIHLIRVWGIDAFPFTNG 454

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RTN LL KNWPESTLFKF  QPRL +WVNQ+RNIIFYGGKDPNWIQQFE+++ EIKNDPY
Sbjct: 455 RTNALLAKNWPESTLFKFIDQPRLMNWVNQDRNIIFYGGKDPNWIQQFEERIEEIKNDPY 514

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           IK++ NTFEIIRVGQ +K ++N  TLT  FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 515 IKERRNTFEIIRVGQKLKEDSNDITLTARFWLTQWGYFVIKSQLKGSSATETTEDILRLI 574

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAI+AVG  PLLVGRGNLI+GVLQDFN+WKR +N+K F  AF DYF ELNLKF
Sbjct: 575 SYENENGWAIVAVGSAPLLVGRGNLIMGVLQDFNKWKRSMNMKIFPDAFRDYFNELNLKF 634

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           HTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL+
Sbjct: 635 HTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPELS 676

BLAST of Lsi09G012660 vs. ExPASy TrEMBL
Match: A0A6J1EX23 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111436942 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.0e-192
Identity = 321/405 (79.26%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EIDLYRWLVDHIDNFPTE+TLVVPKLI+G+   KPFIDGSTRL+VSVEDAL  KNVIL
Sbjct: 290 QEEIDLYRWLVDHIDNFPTELTLVVPKLIEGKVEAKPFIDGSTRLQVSVEDALRDKNVIL 349

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQE--EEEEEVAKKKYEYLSS 121
           +IS LDIS+DDIRA+  +YNEV+K DKYK+VWIPV+   S E  +EEE+ A+KKYEYLSS
Sbjct: 350 LISGLDISDDDIRAVDLVYNEVRKEDKYKIVWIPVVPEHSLEHSQEEEKEARKKYEYLSS 409

Query: 122 LMKWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFT 181
           LMKWYIVPYTT+IAG RYLEENWQLRQDPLV+V++S S V+FTNAIHLIRVWGTEAIPFT
Sbjct: 410 LMKWYIVPYTTKIAGWRYLEENWQLRQDPLVVVMNSQSRVEFTNAIHLIRVWGTEAIPFT 469

Query: 182 QGRTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKND 241
            GRT+ LL KNWPESTL KF +QPRL SWVNQ+R IIFYGGKDPNWIQQFE+KVVEIKND
Sbjct: 470 NGRTHALLEKNWPESTLLKFIEQPRLRSWVNQDRTIIFYGGKDPNWIQQFEEKVVEIKND 529

Query: 242 PYIKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILR 301
           PYI +KGNTFEI+RVG+ I  E N FTLTP FWITQW YFVIKS+++GS+A ETT+DILR
Sbjct: 530 PYIIEKGNTFEIVRVGKKI-NEVNDFTLTPRFWITQWGYFVIKSRIKGSSAAETTEDILR 589

Query: 302 LISYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNL 361
           LISYENENGWA+L+VG  PLLVGRGNLILGVL+DFN+WKR +NIK F  AF DYF ELNL
Sbjct: 590 LISYENENGWAVLSVGSAPLLVGRGNLILGVLEDFNQWKRTLNIKAFPDAFKDYFNELNL 649

Query: 362 KFHTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPEL 405
           KFHTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL
Sbjct: 650 KFHTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPEL 693

BLAST of Lsi09G012660 vs. NCBI nr
Match: XP_038906603.1 (protein SIEVE ELEMENT OCCLUSION B-like [Benincasa hispida])

HSP 1 Score: 711.1 bits (1834), Expect = 5.6e-201
Identity = 333/404 (82.43%), Postives = 374/404 (92.57%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EIDLYRWLVDHIDNFPTEITLVVPKLI+G+   KPFIDGSTR++VSVEDAL  KNVIL
Sbjct: 288 QEEIDLYRWLVDHIDNFPTEITLVVPKLIEGKVDAKPFIDGSTRMQVSVEDALRDKNVIL 347

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           IIS LDISEDDIRALHSIY+EV K D+YK+VWIPVITVESQEEEEE  A+KKYEY+SS M
Sbjct: 348 IISGLDISEDDIRALHSIYDEVNKKDEYKIVWIPVITVESQEEEEE--ARKKYEYISSSM 407

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYTT+IAG RYLEENWQLRQDPLV+V++S S V+FTNAIHLIRVWGTEA+PFT G
Sbjct: 408 KWYIVPYTTKIAGWRYLEENWQLRQDPLVVVMNSKSRVEFTNAIHLIRVWGTEALPFTNG 467

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RT+ LL K+WPESTLFKF +QPRL +WVNQER+IIFYGGKDPNWIQ+FE+KVVEIKNDPY
Sbjct: 468 RTHALLGKHWPESTLFKFIEQPRLQTWVNQERSIIFYGGKDPNWIQEFEEKVVEIKNDPY 527

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           +K+KGNTFEIIRVG+NIKGEN+ FTLTP FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 528 VKEKGNTFEIIRVGRNIKGENHDFTLTPRFWMTQWGYFVIKSQLKGSSATETTEDILRLI 587

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAILA+G  PLLVGRGNLILGVL+DFN+WKR++NI+ F  AF DYF ELNLKF
Sbjct: 588 SYENENGWAILAIGSAPLLVGRGNLILGVLKDFNKWKRNMNIQAFPDAFRDYFNELNLKF 647

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           HTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL+
Sbjct: 648 HTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPELS 689

BLAST of Lsi09G012660 vs. NCBI nr
Match: XP_004150407.1 (protein SIEVE ELEMENT OCCLUSION B [Cucumis sativus])

HSP 1 Score: 697.6 bits (1799), Expect = 6.4e-197
Identity = 326/404 (80.69%), Postives = 365/404 (90.35%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           +DEIDLYRWLVDHIDNFPTEIT VVPKLI+G+   KPFIDGST+L+VSVED L  KNVIL
Sbjct: 289 QDEIDLYRWLVDHIDNFPTEITAVVPKLIEGKFDAKPFIDGSTKLQVSVEDGLRDKNVIL 348

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           +IS LDISEDDIRALHSIYNEVK+ DKYK+VWIPVITVE+++EEEE  A+KKYEY SSLM
Sbjct: 349 VISGLDISEDDIRALHSIYNEVKREDKYKIVWIPVITVETEDEEEE--ARKKYEYASSLM 408

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYT +IAG RYLEENWQLRQDPL++V++S S V+F NAIHLIRVWG +AIPFT G
Sbjct: 409 KWYIVPYTRKIAGWRYLEENWQLRQDPLIVVMNSKSRVEFNNAIHLIRVWGIDAIPFTNG 468

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RTN LL KNWPESTLFKF  QPRL +WVNQERNIIFYGGK+P WIQQFE ++VEIKNDPY
Sbjct: 469 RTNALLAKNWPESTLFKFIDQPRLMNWVNQERNIIFYGGKEPKWIQQFEDRIVEIKNDPY 528

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           +K+KGNTFEIIRVGQNIKG++N FTLTP FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 529 LKEKGNTFEIIRVGQNIKGDSNDFTLTPQFWLTQWGYFVIKSQLKGSSATETTEDILRLI 588

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAI+AVG TPLLVGRGNLI+GVLQDFN+WKR++NIK F  AF DYF ELNL F
Sbjct: 589 SYENENGWAIVAVGSTPLLVGRGNLIMGVLQDFNKWKRNMNIKAFPDAFRDYFNELNLNF 648

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           H C+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGR EL+
Sbjct: 649 HICERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRYELS 690

BLAST of Lsi09G012660 vs. NCBI nr
Match: KAE8651827.1 (hypothetical protein Csa_006561 [Cucumis sativus])

HSP 1 Score: 697.6 bits (1799), Expect = 6.4e-197
Identity = 326/404 (80.69%), Postives = 365/404 (90.35%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           +DEIDLYRWLVDHIDNFPTEIT VVPKLI+G+   KPFIDGST+L+VSVED L  KNVIL
Sbjct: 324 QDEIDLYRWLVDHIDNFPTEITAVVPKLIEGKFDAKPFIDGSTKLQVSVEDGLRDKNVIL 383

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           +IS LDISEDDIRALHSIYNEVK+ DKYK+VWIPVITVE+++EEEE  A+KKYEY SSLM
Sbjct: 384 VISGLDISEDDIRALHSIYNEVKREDKYKIVWIPVITVETEDEEEE--ARKKYEYASSLM 443

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYT +IAG RYLEENWQLRQDPL++V++S S V+F NAIHLIRVWG +AIPFT G
Sbjct: 444 KWYIVPYTRKIAGWRYLEENWQLRQDPLIVVMNSKSRVEFNNAIHLIRVWGIDAIPFTNG 503

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RTN LL KNWPESTLFKF  QPRL +WVNQERNIIFYGGK+P WIQQFE ++VEIKNDPY
Sbjct: 504 RTNALLAKNWPESTLFKFIDQPRLMNWVNQERNIIFYGGKEPKWIQQFEDRIVEIKNDPY 563

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           +K+KGNTFEIIRVGQNIKG++N FTLTP FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 564 LKEKGNTFEIIRVGQNIKGDSNDFTLTPQFWLTQWGYFVIKSQLKGSSATETTEDILRLI 623

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAI+AVG TPLLVGRGNLI+GVLQDFN+WKR++NIK F  AF DYF ELNL F
Sbjct: 624 SYENENGWAIVAVGSTPLLVGRGNLIMGVLQDFNKWKRNMNIKAFPDAFRDYFNELNLNF 683

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           H C+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGR EL+
Sbjct: 684 HICERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRYELS 725

BLAST of Lsi09G012660 vs. NCBI nr
Match: XP_023000469.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita maxima])

HSP 1 Score: 687.2 bits (1772), Expect = 8.6e-194
Identity = 326/405 (80.49%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EIDLYRWLVDHIDNFPTEITLV+PKL++G+   KPFIDGSTRL+VSVED L  KNVIL
Sbjct: 290 QEEIDLYRWLVDHIDNFPTEITLVLPKLVEGKVEAKPFIDGSTRLQVSVEDGLRDKNVIL 349

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQE--EEEEEVAKKKYEYLSS 121
           +IS LDISEDDIRALH +YNEV+K DKYK+VWIPVI   S E  +EEEE A+KKYEYLSS
Sbjct: 350 LISGLDISEDDIRALHLVYNEVRKEDKYKIVWIPVIHEHSPEHSQEEEEEARKKYEYLSS 409

Query: 122 LMKWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFT 181
           LMKWYIVPYTT+IAG RYLEENWQLRQDPLV+V++S S V+FTNAIHLIRVWGTEAIPFT
Sbjct: 410 LMKWYIVPYTTKIAGWRYLEENWQLRQDPLVVVMNSQSRVEFTNAIHLIRVWGTEAIPFT 469

Query: 182 QGRTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKND 241
            GRT+ LL KNWPESTL KF +QPRL SWVNQ+R IIFYGGKDPNWIQQFE+KVVEIKND
Sbjct: 470 NGRTHALLEKNWPESTLLKFIEQPRLRSWVNQDRAIIFYGGKDPNWIQQFEEKVVEIKND 529

Query: 242 PYIKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILR 301
           PYIK KGNTFEI+RVG+ I  E N F LTP FWITQW YFVIKSQL+GS+A ETT+DILR
Sbjct: 530 PYIKDKGNTFEIVRVGKKI-NEVNDFALTPLFWITQWGYFVIKSQLKGSSAAETTEDILR 589

Query: 302 LISYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNL 361
           LISYENENGWA+LAVG  PLLVGRGNLILGVL+DFN+WKR++NI+ F  AF DYF ELNL
Sbjct: 590 LISYENENGWAVLAVGSAPLLVGRGNLILGVLEDFNKWKRNLNIEAFPDAFKDYFNELNL 649

Query: 362 KFHTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPEL 405
           KFHTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL
Sbjct: 650 KFHTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPEL 693

BLAST of Lsi09G012660 vs. NCBI nr
Match: KAA0057512.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucumis melo var. makuwa] >TYK08193.1 protein SIEVE ELEMENT OCCLUSION B-like [Cucumis melo var. makuwa])

HSP 1 Score: 684.9 bits (1766), Expect = 4.3e-193
Identity = 318/404 (78.71%), Postives = 364/404 (90.10%), Query Frame = 0

Query: 2   KDEIDLYRWLVDHIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVIL 61
           ++EI+LY+WLVDHIDNFPTEITLVVPKLI+G+   KPFIDGST+L+VS+ED L  KNVIL
Sbjct: 272 REEIELYKWLVDHIDNFPTEITLVVPKLIEGKFDAKPFIDGSTKLQVSIEDGLRDKNVIL 331

Query: 62  IISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLM 121
           +IS LDISEDDIRALHSIY+EVKK DKYK+VWIPVITVE+Q+EEE+  A+KKYEY+SSLM
Sbjct: 332 VISGLDISEDDIRALHSIYDEVKKEDKYKIVWIPVITVETQDEEEK--ARKKYEYVSSLM 391

Query: 122 KWYIVPYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQG 181
           KWYIVPYT++IAG RYLEENWQLRQDPLV+V++S S V+F NAIHLIRVWG +A PFT G
Sbjct: 392 KWYIVPYTSKIAGWRYLEENWQLRQDPLVVVMNSKSRVEFNNAIHLIRVWGIDAFPFTNG 451

Query: 182 RTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKNDPY 241
           RTN LL KNWPESTLFKF  QPRL +WVNQ+RNIIFYGGKDPNWIQQFE+++ EIKNDPY
Sbjct: 452 RTNALLAKNWPESTLFKFIDQPRLMNWVNQDRNIIFYGGKDPNWIQQFEERIEEIKNDPY 511

Query: 242 IKKKGNTFEIIRVGQNIKGENNYFTLTPHFWITQWSYFVIKSQLRGSNATETTDDILRLI 301
           IK++ NTFEIIRVGQ +K ++N  TLT  FW+TQW YFVIKSQL+GS+ATETT+DILRLI
Sbjct: 512 IKERRNTFEIIRVGQKLKEDSNDITLTARFWLTQWGYFVIKSQLKGSSATETTEDILRLI 571

Query: 302 SYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKF 361
           SYENENGWAI+AVG  PLLVGRGNLI+GVLQDFN+WKR +N+K F  AF DYF ELNLKF
Sbjct: 572 SYENENGWAIVAVGSAPLLVGRGNLIMGVLQDFNKWKRSMNMKIFPDAFRDYFNELNLKF 631

Query: 362 HTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKCNHGRPELT 406
           HTC+RMTLPGFSGWIPMI+NCPECPRFME GISFKCNHGRPEL+
Sbjct: 632 HTCERMTLPGFSGWIPMIVNCPECPRFMETGISFKCNHGRPELS 673

BLAST of Lsi09G012660 vs. TAIR 10
Match: AT3G01680.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01670.1); Has 122 Blast hits to 112 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-41
Identity = 122/422 (28.91%), Postives = 191/422 (45.26%), Query Frame = 0

Query: 14  HIDNFPTEITLVVPKLIKGRASTKPFIDGSTRLEVSVEDALEGKNVILIISRLDISEDDI 73
           HIDN      LV PK         P  DG T+ +V + D L  K V+L+IS L+I +D++
Sbjct: 320 HIDNMKILTALVHPK-----PHITPLQDGLTKRKVHL-DVLRRKTVLLLISDLNILQDEL 379

Query: 74  RALHSIYNEVKKG---------DKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLMKWY 133
                IY E ++            Y++VW+PV+    ++ E   + +KK+E L   M WY
Sbjct: 380 SIFEQIYTESRRNLVGVDGKSHMPYEVVWVPVVD-PIEDFERSPILQKKFEDLRDPMPWY 439

Query: 134 IV--PYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQGR 193
            V  P   E   + ++   W     P+++VID        NA+H+I +WGTEA PFT+ R
Sbjct: 440 SVDSPKLIERHVVEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSR 499

Query: 194 TNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQFEKKVVEIKND--- 253
             EL R+      L        + +W+  +  I  YGG D +WI++F         D   
Sbjct: 500 EEELWRRETFSLNLIVDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRFTMAAKATAKDSNV 559

Query: 254 ----PYIKKKGNTF--EIIRVGQNIKGENNYFT-----LTPHFWITQWSYFVIKSQL-RG 313
                Y+ K+ ++   +I R+ + I+ EN   +     L   FW    S    K QL + 
Sbjct: 560 NLEMAYVGKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKA 619

Query: 314 SNATETTDDILRLISYENENGWAILAVGLTPLLVGRGNLILGVLQDFNEWKRDINIKTFS 373
            +  +    I +++SY+   GWA+L+ G   +++  G +   +      WK  +  K ++
Sbjct: 620 DDHDDVMQGIKKILSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYT 679

Query: 374 KAFIDYFTELNLK----------FHTCDRMTLPGFSGWIPMIINCPECPRFMEAGISFKC 400
           KA  D+  +  L+          FH   R      SG IP  +NC EC R ME  +SF C
Sbjct: 680 KAMSDHHHDEVLRETGKPCGHFDFHITAR------SGRIPEKMNCFECQRPMEKYMSFSC 728

BLAST of Lsi09G012660 vs. TAIR 10
Match: AT3G01670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 121 Blast hits to 111 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 121; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-31
Identity = 101/374 (27.01%), Postives = 171/374 (45.72%), Query Frame = 0

Query: 52  DALEGKNVILIISRLDISEDDIRALHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAK 111
           + L  K+V+L+IS L+  E ++  L S+Y E  +   ++++W+PV      ++   E   
Sbjct: 455 NVLTQKHVLLLISDLENIEKELYILESLYTEAWQ-QSFEILWVPV------QDFWTEADD 514

Query: 112 KKYEYLSSLMKWYIV--PYTTEIAGLRYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIR 171
            K+E L   M+WY++  P     A +R++ E W  +  P+++ +D    V  TNA  ++ 
Sbjct: 515 AKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVW 574

Query: 172 VWGTEAIPFTQGRTNEL-LRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGGKDPNWIQQ 231
           +W   A PFT  R  +L   + W    L   T    LN  V+  + I  YGG+D  WI+ 
Sbjct: 575 IWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLVD-GKYICLYGGEDMQWIKN 634

Query: 232 FEK------KVVEIKND-PYIKKKGNTFEIIRVGQNIKGENNYFTLTP-----HFWITQW 291
           F        K   I+ +  Y+ K+     I  +   I+ EN   TL        FW    
Sbjct: 635 FTSLWRNVAKAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVE 694

Query: 292 SYFVIKSQLRGSNATE------------TTDDILRLISYENE-NGWAILAVGLTPLLVGR 351
           S +  K ++  ++  +               +++ ++ Y  E +GW +++     ++  +
Sbjct: 695 SMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAK 754

Query: 352 GNLILGVLQDFNEWKRDINIKTFSKAFIDYFTELNLKFHTCDRMTLPGFSGWIPMIINCP 398
           GNL    L +FNEW+ +I  K F  A  D+   + L  H C R  LP  +G IP  + C 
Sbjct: 755 GNLFSRGLAEFNEWEVNIPTKGFLTALNDHLL-MRLPPHHCTRFMLPETAGIIPNEVECT 814

BLAST of Lsi09G012660 vs. TAIR 10
Match: AT1G67790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 208 Blast hits to 125 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-11
Identity = 62/292 (21.23%), Postives = 130/292 (44.52%), Query Frame = 0

Query: 47  EVSVEDALEGKNVILIISRLDISEDDIRALHSIY---NEVKKGDKYKMVWIPVITVESQE 106
           ++S+ + ++ K  +L++S+  + E     L  +Y   +       Y+++W+P+ + +   
Sbjct: 229 QISITE-VQDKVTLLLLSKPPV-EPLFFLLQQLYDHPSNTNTEQNYEIIWVPIPSSQKWT 288

Query: 107 EEEEEVAKKKYEYLSSLMKWYIV--PYTTEIAGLRYLEENWQLR-QDPLVIVIDSTSGVK 166
           +EE+E+    +++ S+ + W  V  P+      L + ++ W  +  + +++VIDS     
Sbjct: 289 DEEKEI----FDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDNEAMLVVIDSNGRFV 348

Query: 167 FTNAIHLIRVWGTEAIPFTQGRTNELLRKNWPESTLFKFTKQPRLNSWVNQERNIIFYGG 226
             NA+ ++ +WG +A PF+  R +EL +++     L      P       + R I  +G 
Sbjct: 349 NMNAMDMVLIWGVKAYPFSVSREDELWKEHGWSINLLLDGIHPTF-----EGREICIFGS 408

Query: 227 KDPNWIQQFEKKVVEIKNDPYIKKKGNTFEIIRVGQNIKGENNYF--------TLTPHFW 286
           ++ +WI +F     +I+N       G   E+I +    + E            TL   FW
Sbjct: 409 ENLDWIDEFVSLARKIQN------LGFQLELIYLSNQRRDERAMEESSILFSPTLQQLFW 468

Query: 287 ITQWSYFVIKSQLR-----GSNATETTDDILRLI--SYENENGWAILAVGLT 318
           +   S  + +S+L+      S      +++  L+   Y    GW I+  G T
Sbjct: 469 LRLES--IERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGWGIIGNGST 501

BLAST of Lsi09G012660 vs. TAIR 10
Match: AT1G60420.1 (DC1 domain-containing protein )

HSP 1 Score: 43.1 bits (100), Expect = 6.1e-04
Identity = 30/111 (27.03%), Postives = 56/111 (50.45%), Query Frame = 0

Query: 76  LHSIYNEVKKGDKYKMVWIPVITVESQEEEEEEVAKKKYEYLSSLMKWYIVPYTTEIAGL 135
           L  +YNE+     +++V++      S +E+EE       +Y    M W  VP+T      
Sbjct: 65  LVEVYNELSSKVGFEIVFV------SGDEDEESFG----DYFRK-MPWLAVPFTDSETRD 124

Query: 136 RYLEENWQLRQDPLVIVIDSTSGVKFTNAIHLIRVWGTEAIPFTQGRTNEL 187
           R L+E +++R  P ++++D    +   N + +IR +G +A PFT  +  E+
Sbjct: 125 R-LDELFKVRGIPNLVMVDDHGKLVNENGVGVIRSYGADAYPFTPEKMKEI 163

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SS871.8e-4028.91Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 S... [more]
Q93XX21.7e-3027.01Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 S... [more]
Q9FXE21.5e-1021.23Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 S... [more]
Q7Y0E81.6e-0429.36Probable nucleoredoxin 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Q7Y0F25.9e-0429.36Probable nucleoredoxin 1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0405... [more]
Match NameE-valueIdentityDescription
A0A0A0LNQ73.1e-19780.69Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G193350 PE=4 SV=1[more]
A0A6J1KK254.2e-19480.49protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC1114947... [more]
A0A5A7UNT22.1e-19378.71Protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CN792.1e-19378.71protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo OX=3656 GN=LOC103502853 P... [more]
A0A6J1EX233.0e-19279.26protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC11143... [more]
Match NameE-valueIdentityDescription
XP_038906603.15.6e-20182.43protein SIEVE ELEMENT OCCLUSION B-like [Benincasa hispida][more]
XP_004150407.16.4e-19780.69protein SIEVE ELEMENT OCCLUSION B [Cucumis sativus][more]
KAE8651827.16.4e-19780.69hypothetical protein Csa_006561 [Cucumis sativus][more]
XP_023000469.18.6e-19480.49protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita maxima][more]
KAA0057512.14.3e-19378.71protein SIEVE ELEMENT OCCLUSION B-like [Cucumis melo var. makuwa] >TYK08193.1 pr... [more]
Match NameE-valueIdentityDescription
AT3G01680.11.3e-4128.91CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640);... [more]
AT3G01670.11.2e-3127.01unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G67790.11.0e-1121.23unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G60420.16.1e-0427.03DC1 domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027944Sieve element occlusion, C-terminalPFAMPF14577SEO_Ccoord: 202..399
e-value: 1.0E-31
score: 110.2
IPR039299Protein SIEVE ELEMENT OCCLUSIONPANTHERPTHR33232PROTEIN SIEVE ELEMENT OCCLUSION B-LIKEcoord: 3..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G012660.1Lsi09G012660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010088 phloem development