Lsi09G012650 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi09G012650 vs. ExPASy Swiss-Prot
Match: Q9SS87 (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.1e-55
Identity = 177/647 (27.36%), Postives = 289/647 (44.67%), Query Frame = 0

Query: 96  SQIDPPICTLHQISTEMTCKDPGVETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGV 155
           S +D     + +++ E+  K      +H+ T+ +   L+ + WD K VL   AFA NYG 
Sbjct: 101 SVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQWDGKLVLTLAAFALNYGE 160

Query: 156 LWHLDNYSHSDPLAKSLATIKRVALL-RKELDSVKYGQVFFNPNSMIYNCLKVIKYINEF 215
            W L  +   + LAKSLA +K V +  R  L+SV  G      N +I     V   + E 
Sbjct: 161 FWLLVQFYSKNQLAKSLAMLKLVPVQNRVTLESVSQGL-----NDLIREMKSVTACVVEL 220

Query: 216 RNL-SKYDTKDVPELSAALRQIPLVSYWVIHTLVASSIELHCYLSGVQGHTLKYLN-DLS 275
             L  +Y T DVP+LS  L  IP+  YW I +++A   +++  +    GH +     DL 
Sbjct: 221 SELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQIN--MITAMGHEMMNTQMDLW 280

Query: 276 E--KVGSVLLTLENHL-HFIR------EQQEEVELYRWLVDQTDHFPTDVTLFLSKLIDG 335
           E   + + L  + +HL   +R      E+Q   E  + L    D    D    L+ L+  
Sbjct: 281 ETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTTHIDNMKILTALVHP 340

Query: 336 KHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDISKEDLEILHSIYNEV-------- 395
           K    PL +  T+ + ++ D ++ K ++L++S  L+I +++L I   IY E         
Sbjct: 341 KPHITPLQDGLTKRKVHL-DVLRRKTVLLLISD-LNILQDELSIFEQIYTESRRNLVGVD 400

Query: 396 -KKENKFEMVWIPVITDGPNEGDEEA------YEYLKSTMKWYIV--PFSTQIAGVRFLE 455
            K    +E+VW+PV+   P E  E +      +E L+  M WY V  P   +   V F+ 
Sbjct: 401 GKSHMPYEVVWVPVV--DPIEDFERSPILQKKFEDLRDPMPWYSVDSPKLIERHVVEFMR 460

Query: 456 ERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWIDSTVVKF 515
            RW      ++VV++ Q      NA+H+  +W  EA PFT  R + L  +      ++  
Sbjct: 461 GRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRRETFSLNLI-- 520

Query: 516 TDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFELVPVGMN 575
            D         I  +  +  YGG +  WI++F     TM    T +D  +  E+  VG  
Sbjct: 521 VDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRF-----TMAAKATAKDSNVNLEMAYVGKR 580

Query: 576 NKGE--------------------LDPTIMFRFWMAQRSYFILKHQLHGSTAAED----I 635
           N                        +P +M+ FW    S    K QL  +   +D    I
Sbjct: 581 NHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVMQGI 640

Query: 636 SRLISYENEYGWAIIAKGPTVTFVGGGDLILKAMNEF-QIWKKNLRRVGFTGSFKDYYDE 685
            +++SY+   GWA+++KGP +  +  G  I + M+ + + WK ++   G+T +  D++ +
Sbjct: 641 KKILSYDKLGGWALLSKGPEIVMIAHG-AIERTMSVYDRTWKTHVPTKGYTKAMSDHHHD 700

BLAST of Lsi09G012650 vs. ExPASy Swiss-Prot
Match: Q93XX2 (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.8e-40
Identity = 186/733 (25.38%), Postives = 311/733 (42.43%), Query Frame = 0

Query: 31  LSDD-VITNRIFTINSDDETIKIDIDNYILFIESVIKTSDQIAVASNWAKGSKGHFVLAD 90
           LSDD V+ +R+   +S D  I  D+ + +  +  + K+     V S  +   K   V  D
Sbjct: 136 LSDDRVMADRVLKTHSPD-MIFFDVTSLLSVVNDIFKSH----VPSIDSSAPKPSLVFKD 195

Query: 91  --DSLKYPSQIDPPICTLHQISTEMTCK------DPGVETT-------HQTTLDILNKLA 150
             D   + +  D     + QIS E+ CK        G+ T+       + TT  +L+ ++
Sbjct: 196 YADHTSFETFAD----LIDQISCEIDCKCLHGGESHGMMTSGLHLDSRNTTTFSVLSLVS 255

Query: 151 RYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVALLRKELDSVKYGQVF 210
           +Y WDAK VL+ +A A  YGV   L     ++ L KSLA IK++              +F
Sbjct: 256 KYRWDAKLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQLP------------SIF 315

Query: 211 FNPNSMIYNCLKVIKYINEFRNLSK--YDTKDVP--ELSAALR-QIPLVSYWVI------ 270
              N++     K    + +  +L+    D   +P   ++AA    IP   YW++      
Sbjct: 316 SRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHITAAFTDHIPTAVYWIVRCVLIC 375

Query: 271 --HTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLL--------TLENHLHFIREQQ 330
             H   AS  +    +S ++   +   ++   K+ + LL        T+E  +    E Q
Sbjct: 376 VSHISGASGFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMTIEEGI-IEEEYQ 435

Query: 331 EEVELYRWLVDQTDHFPTDVTLFLSKLID------GKHKARPLINCSTQLEEYIEDFMKE 390
           E ++ +  ++   D  P    L L + ID      G  K R  IN  TQ           
Sbjct: 436 ELIQTFTTII-HVDVVPP--LLRLLRPIDFLYHGAGVSKRRVGINVLTQ----------- 495

Query: 391 KKLILIVSKRLDISKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKST 450
           K ++L++S   +I KE L IL S+Y E  +++ FE++W+PV  D   E D+  +E L   
Sbjct: 496 KHVLLLISDLENIEKE-LYILESLYTEAWQQS-FEILWVPV-QDFWTEADDAKFEALHMN 555

Query: 451 MKWYIV--PFSTQIAGVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPF 510
           M+WY++  P   + A +RF+ E W  +   ++V L+ + +V  +NA  +  +W+  A PF
Sbjct: 556 MRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVWIWQPFAHPF 615

Query: 511 TYDRAKALLN-KNWIDSTVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAET 570
           T  R + L + + W    ++  TD   L  LV     + +  YGG + +WIK F     T
Sbjct: 616 TTARERDLWSEQEWNLEFLIDGTDPHSLNQLV---DGKYICLYGGEDMQWIKNF-----T 675

Query: 571 MKRDPTTRDEGITFELVPVGMNN-KGELDPTI-----------------MFRFWMAQRSY 630
                  +   I  E+V VG  N K  + P I                 ++ FW    S 
Sbjct: 676 SLWRNVAKAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVESM 735

Query: 631 FILKHQL---HGSTAAE------------DISRLISYENE-YGWAIIAKGPTVTFVGGGD 684
           +  K ++   HG    E            ++  ++ Y  E  GW +++K   +     G+
Sbjct: 736 WESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAKGN 795

BLAST of Lsi09G012650 vs. ExPASy Swiss-Prot
Match: Q9FXE2 (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 9.7e-23
Identity = 128/608 (21.05%), Postives = 246/608 (40.46%), Query Frame = 0

Query: 105 LHQISTEMTCKDPGVETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSH 164
           + +IS +M C   G     + T+ + + L  Y WDAKAVL+    A  YG L    + + 
Sbjct: 77  IFRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAVLVLGVLAATYGGLLLPVHLAI 136

Query: 165 SDPLAKSLATIKRVALLRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTK- 224
            DP+A S+A + ++ + R      K+     + N +I   + V K I +F  +     K 
Sbjct: 137 CDPVAASIAKLNQLPIER-----TKFRPWLESLNLLIKAMVDVTKCIIKFEKIPFKQAKL 196

Query: 225 DVPELSAALRQIPLVSYWVIHTLVASSIELHCYLSGVQG-HTLKYLNDLS---------- 284
           D   L   L  I L +Y V+ + +    ++  +    Q   + K   +LS          
Sbjct: 197 DNNILGETLSNIYLTTYRVVKSALTCMQQIPYFKQTQQAKKSRKTAAELSIESRRAAGEL 256

Query: 285 EKVGSVLLTLENHLHFIREQ-----QEEV-ELYRWLVDQTDHFPTDVTLFLSKLIDGKHK 344
             +G  LL +   L+   E      +EE+ +  R +  +T     DV   L  L D    
Sbjct: 257 SSLGYQLLNIHTRLNKQVEDCSTQIEEEINQRLRNINIETHQDNQDVLHLLFSLQDD--- 316

Query: 345 ARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDISKEDLEILHSIYNEVKKENKFEMVWI 404
             PL   S Q+   I +   +  L+L+    ++     L+ L+   +    E  +E++W+
Sbjct: 317 -LPLQQYSRQIS--ITEVQDKVTLLLLSKPPVEPLFFLLQQLYDHPSNTNTEQNYEIIWV 376

Query: 405 PVITDGP-NEGDEEAYEYLKSTMKWYIV--PFSTQIAGVRFLEERWELRE-DILMVVLNT 464
           P+ +     + ++E +++  +++ W  V  P+      + F ++ W  ++ + ++VV+++
Sbjct: 377 PIPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDNEAMLVVIDS 436

Query: 465 QSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWIDSTVVKFTDQPRLRTLVVINQER 524
             +    NA+ +  +W  +A PF+  R   L  ++     ++     P         + R
Sbjct: 437 NGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEHGWSINLLLDGIHPTF-------EGR 496

Query: 525 NVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFELVPVGMNNKGE---------LDP 584
            +  +G  N  WI +F   A  +      ++ G   EL+ +    + E           P
Sbjct: 497 EICIFGSENLDWIDEFVSLARKI------QNLGFQLELIYLSNQRRDERAMEESSILFSP 556

Query: 585 TIMFRFWMAQRSY------FILKHQLHGSTAAEDISRLI--SYENEYGWAIIAKGPTVTF 644
           T+   FW+   S        I+          E++  L+   Y    GW II  G T   
Sbjct: 557 TLQQLFWLRLESIERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGWGIIGNGSTAET 616

Query: 645 VGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATS-LHCTHVNIIGFSGWIPL- 672
           V  G+ + + M +   W +  + +GFT +      E+ A      +H  ++ F   + + 
Sbjct: 617 V-DGEKMTERMRKIVRWGEYAKGLGFTEAI-----EIAAEKPCELSHTAVVPFEEALTMK 654

BLAST of Lsi09G012650 vs. ExPASy TrEMBL
Match: A0A5A7UTQ8 (Protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00130 PE=4 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 608/691 (87.99%), Postives = 646/691 (93.49%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQ-SLKHLSDDVITNRIFTINSDDETIKIDIDNYIL 60
           MS FRPKTSATALALAL QE DKEE+ SLKHLSDD ITNRIFTINSD ET+KIDID+YIL
Sbjct: 1   MSIFRPKTSATALALALPQEIDKEEEKSLKHLSDDFITNRIFTINSDAETMKIDIDSYIL 60

Query: 61  FIESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGV 120
           FIESVIK+SD+IAVAS+WAKGSKGHF LAD++LKYP+QIDPPICTLHQISTEM CKDPG 
Sbjct: 61  FIESVIKSSDKIAVASHWAKGSKGHFELADETLKYPTQIDPPICTLHQISTEMICKDPGA 120

Query: 121 ETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVA 180
           ET HQTTLDIL KLA+Y WDAKAVLIFTAFAT YGVLWHLDNYSHSD LAKSLATIKRVA
Sbjct: 121 ETAHQTTLDILQKLAKYSWDAKAVLIFTAFATTYGVLWHLDNYSHSDTLAKSLATIKRVA 180

Query: 181 LLRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVS 240
           LLRKELDSVKYGQVFFNPNSMIYNC+K IKYINEFRNLSKYDTKDVPELSAALRQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNPNSMIYNCMKAIKYINEFRNLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELY 300
           YW+IHTLVASSIELHCYLSGVQG T KYLN+LSEK  SVLLTLENHL  IREQ EEVELY
Sbjct: 241 YWIIHTLVASSIELHCYLSGVQGQTHKYLNELSEKSESVLLTLENHLQLIREQIEEVELY 300

Query: 301 RWLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLD 360
           RWLVDQTDHFPTD+TLFLSKLIDGKHKARPLINCSTQLEEYIEDF+KEKKLILIVSKRLD
Sbjct: 301 RWLVDQTDHFPTDITLFLSKLIDGKHKARPLINCSTQLEEYIEDFLKEKKLILIVSKRLD 360

Query: 361 ISKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQI 420
           IS EDLEIL+SIYNEVKKENKFEMVWIPVI D P +GDEE YEYLKS MKWY+VPF+T+I
Sbjct: 361 ISTEDLEILYSIYNEVKKENKFEMVWIPVIPDPPMDGDEEGYEYLKSVMKWYMVPFNTKI 420

Query: 421 AGVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWI 480
           AG+RFLEERWELREDILMVVLNTQSKVEFSN IHL R+WEKEALPFTYDRAKALL KNWI
Sbjct: 421 AGMRFLEERWELREDILMVVLNTQSKVEFSNGIHLARIWEKEALPFTYDRAKALLKKNWI 480

Query: 481 DSTVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFE 540
           DSTVVKFTDQPRLR+LVVINQERNVIFYGGHN RWIKKFEESAE MKRDPTTR+EGITFE
Sbjct: 481 DSTVVKFTDQPRLRSLVVINQERNVIFYGGHNLRWIKKFEESAEVMKRDPTTREEGITFE 540

Query: 541 LVPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAK 600
           L PVG+ NKGE DP +MFRFWMAQRSYFILKHQL GSTA EDISRLISYE E GWAII K
Sbjct: 541 LAPVGL-NKGEQDPALMFRFWMAQRSYFILKHQLQGSTATEDISRLISYETEDGWAIITK 600

Query: 601 GPTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSG 660
           GPTV FV GGDLILKAM+EF +WKKN+RR+GF+GSFK+++DELTATSLHCT+VN+IGFSG
Sbjct: 601 GPTVVFVAGGDLILKAMDEFHLWKKNMRRLGFSGSFKEHFDELTATSLHCTNVNLIGFSG 660

Query: 661 WIPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           WIPLF+TCP+CRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLFLTCPMCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lsi09G012650 vs. ExPASy TrEMBL
Match: A0A6J1H3Z4 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111459939 PE=4 SV=1)

HSP 1 Score: 1212.6 bits (3136), Expect = 0.0e+00
Identity = 572/690 (82.90%), Postives = 638/690 (92.46%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILF 60
           MS  RPK+SA ALALALSQ+TDKEE  LKHLSDDVITNRIF   +D+E  KID+DNY+LF
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFTTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGVE 120
           IE+V+K SDQI VAS+WA+GSKGHFVLA+D++ YPS IDPPICTLHQIS+EM CK PGVE
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVAL 180
           T H+TTLDIL+KL RYPW+AKAVLIFTAF TNYGVLWHLDN+SHSDPLAKSLA IKRV L
Sbjct: 121 TVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLAMIKRVGL 180

Query: 181 LRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVSY 240
           LRKEL+SVKYGQVFFN NSMIYNCLK +KYINEF++LSKYDTKDVPELSAALRQIPLVSY
Sbjct: 181 LRKELESVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVSY 240

Query: 241 WVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELYR 300
           W+IH LVASSIELHCYLSGV+G T KYLN+++EKV S++LTLENH+ FIREQQEEVELYR
Sbjct: 241 WIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELYR 300

Query: 301 WLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDI 360
           WLVDQTDHFPTDVTLFL+KLI+GKHKARPLINCSTQLEE+IE+FMKEKKLILIVS+ L+ 
Sbjct: 301 WLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLNF 360

Query: 361 SKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQIA 420
           S+EDLEIL S+Y EVKKENKFEMVWIPVI+D PN+GDEEAYE LKS MKW++VPF+ +IA
Sbjct: 361 SREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKIA 420

Query: 421 GVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWID 480
           GVRFLEERWELRED+L+VVL+TQSKVEFSNAIHLTRVWEKEA+PF+YDR KAL+ KNWID
Sbjct: 421 GVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWID 480

Query: 481 STVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFEL 540
           STV+K+TDQPRL++LVVINQERNVIFYGGHN RW+K FE+SAE MKRDP TR+EGITFEL
Sbjct: 481 STVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFEL 540

Query: 541 VPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAKG 600
           VPVG NNKGE DP +MF FWMAQRSYFILKHQLHGSTA+EDISRLISYE E GWAII KG
Sbjct: 541 VPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITKG 600

Query: 601 PTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSGW 660
           P V F+GGGDLILKA++EFQ+WKKNLRRVGF+GSFKDY+DELTA+SLHCTHVNIIGFSGW
Sbjct: 601 PIVVFIGGGDLILKAIDEFQVWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSGW 660

Query: 661 IPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           IPL ITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 IPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lsi09G012650 vs. ExPASy TrEMBL
Match: A0A6J1KWJ8 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111499359 PE=4 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 567/691 (82.05%), Postives = 637/691 (92.19%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILF 60
           MS  R K+SATALALALSQ+TDKEE  LKHLSDDVITNRIF   +D+E +KID+DNY++F
Sbjct: 1   MSLLRSKSSATALALALSQQTDKEEPDLKHLSDDVITNRIFNFTTDEEMMKIDLDNYVVF 60

Query: 61  IESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQ-IDPPICTLHQISTEMTCKDPGV 120
           IE+V+K SDQI VAS+WA+GSKG+FVLA+D++ YPS  IDPPICTLHQIS+EM CK PGV
Sbjct: 61  IENVLKASDQICVASHWAQGSKGNFVLAEDTMNYPSSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 ETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVA 180
           ET H+TTLDIL+KL RYPW+AKA+LIF AF TNYGVLWHLDN+SHSDPLAKSLA IKRV 
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAMLIFAAFMTNYGVLWHLDNHSHSDPLAKSLAMIKRVG 180

Query: 181 LLRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVS 240
           LLRKEL+SVKYGQVFFN NSMIYNCLK +KYI EF++LSKYDTKDVPELSAALR+IPLVS
Sbjct: 181 LLRKELESVKYGQVFFNQNSMIYNCLKAVKYIIEFKSLSKYDTKDVPELSAALRRIPLVS 240

Query: 241 YWVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELY 300
           YW+IH LVASSIELHCYLSGV+G T KYLN+++EKV +++LTLENH+ FIREQQEEVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSTLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLD 360
           RWLVDQTDHFPTDVTLFL+KLI+GKHKARPLINCSTQLEE+IE+FMKEKKLILIVS+ L+
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 ISKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQI 420
            S+EDLEIL SIY EVKKENKFEMVWIPVI+D PN+GDEEAYE LKS MKW++VPF+T+I
Sbjct: 361 FSREDLEILQSIYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFATKI 420

Query: 421 AGVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWI 480
           AGVRFLEERWELRED+L+VVL+TQSKVEFSNAIHLTRVWEKEA+P +YDR K L+ KNWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPLSYDRVKVLMKKNWI 480

Query: 481 DSTVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFE 540
           DSTV+KFTDQPRL++LVVINQERNVIFYGGHN RW+K FEESAE MKRDP TR+EGITFE
Sbjct: 481 DSTVLKFTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEESAEAMKRDPVTREEGITFE 540

Query: 541 LVPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAK 600
           LVPVG NNKGE DP +MF FWMAQRSYFILKHQLHGSTA+EDISR+ISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRMISYETEKGWAIITK 600

Query: 601 GPTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSG 660
           GPTV F+GGGDLILKA++EFQIWKKNLR+VGF+GSFKDY+DELTA+SLHCTHVNIIGFSG
Sbjct: 601 GPTVVFIGGGDLILKAIDEFQIWKKNLRQVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           WIPL ITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 691

BLAST of Lsi09G012650 vs. ExPASy TrEMBL
Match: A0A6J1EQ44 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111436801 PE=4 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 510/686 (74.34%), Postives = 595/686 (86.73%), Query Frame = 0

Query: 5   RPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILFIESV 64
           RPK S   LALAL Q+TDKE+ +LKHLSDDVI N +FT + DDETIKID+++YI FIE+V
Sbjct: 8   RPKISVVPLALALMQKTDKEQTNLKHLSDDVIMNHLFTKHGDDETIKIDLNDYISFIENV 67

Query: 65  IKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGVETTHQ 124
           IK+SDQI+ AS+WA+GSK H  L+DDS  Y S I+PP+CTLH IS +M CK  G++  H+
Sbjct: 68  IKSSDQISAASHWAQGSKVHVELSDDSFTYLSSIEPPVCTLHDISKQMGCKAAGIDIAHK 127

Query: 125 TTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVALLRKE 184
           TTLDIL+KL +Y W+AKAVLIF AFA NYG LWHLDNYS SDPLAKSLA IKRV  LRKE
Sbjct: 128 TTLDILSKLTKYSWEAKAVLIFAAFAMNYGALWHLDNYSRSDPLAKSLAMIKRVGSLRKE 187

Query: 185 LDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVSYWVIH 244
           LDSV+YGQVFF PNS+IY+CLK IKY+NEF+NLSKYD K+VPELSAALR+IPLVSYW++H
Sbjct: 188 LDSVQYGQVFFGPNSLIYSCLKAIKYMNEFKNLSKYDIKEVPELSAALRRIPLVSYWIVH 247

Query: 245 TLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELYRWLVD 304
            LVASSI+LHCYLSG +G T KYLN+LSEK  S+L+TLE H  FI++QQEEVELYRWLVD
Sbjct: 248 ALVASSIDLHCYLSGTEGQTHKYLNELSEKKRSILVTLEKHHQFIQKQQEEVELYRWLVD 307

Query: 305 QTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDISKED 364
           QTDHFP D+TLFLSKLI GKHKARPLINCST+LEEYIEDF+KEK LILIVSKRLD+SKED
Sbjct: 308 QTDHFPIDITLFLSKLIHGKHKARPLINCSTRLEEYIEDFLKEKNLILIVSKRLDVSKED 367

Query: 365 LEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQIAGVRF 424
           L+ L+ +YNEVKK NK E+VW+PVI+D P EGDEEAYE L S MKWY VPF T++AG+RF
Sbjct: 368 LDSLNVVYNEVKKGNKHEIVWVPVISDPPAEGDEEAYEALISKMKWYAVPFDTKVAGLRF 427

Query: 425 LEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWIDSTVV 484
           LEE+WE+RED+L+VVLNTQSK+EF NA+HLTR+WEKEA+PFTY+RA ALL ++WIDSTVV
Sbjct: 428 LEEKWEVREDLLIVVLNTQSKIEFPNAVHLTRIWEKEAIPFTYERANALLKRSWIDSTVV 487

Query: 485 KFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFELVPVG 544
           KFTDQPRL +L +IN+ER +IFYGGHN  WIK+FE++AE +KRD   R+EGITFELVP+G
Sbjct: 488 KFTDQPRLNSLAMINKERTMIFYGGHNQGWIKRFEDTAEAIKRDLMLREEGITFELVPLG 547

Query: 545 MNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAKGPTVT 604
           +N KGE DPTIM RFW AQRS+FILKHQL GSTA EDISRLISYENE GWAI+ KG TV 
Sbjct: 548 LNQKGEPDPTIMSRFWTAQRSFFILKHQLQGSTATEDISRLISYENEKGWAIVTKGQTVV 607

Query: 605 FVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSGWIPLF 664
            VGGGDLI+KA+ EFQ WKKNLRRVGF+GSFKDY+DELT+ SL CTHVNI+G+SGWIPL 
Sbjct: 608 MVGGGDLIVKALEEFQTWKKNLRRVGFSGSFKDYFDELTSKSLECTHVNIMGYSGWIPLT 667

Query: 665 ITCPVCRRYMGSGIRFTCCHGGPDVL 691
           + CPVCRRYMGSGIRFTCCHG P+VL
Sbjct: 668 VMCPVCRRYMGSGIRFTCCHGRPNVL 693

BLAST of Lsi09G012650 vs. ExPASy TrEMBL
Match: A0A6J1KIF3 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111494730 PE=4 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 5.1e-309
Identity = 503/686 (73.32%), Postives = 593/686 (86.44%), Query Frame = 0

Query: 5   RPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILFIESV 64
           RPK SA  LALAL Q+TDKE+ +LK LSDDVI N +FT + +DETI I++++YI FIE+V
Sbjct: 8   RPKISAVPLALALMQKTDKEQTNLKQLSDDVIMNHLFTKHGEDETININLNDYISFIENV 67

Query: 65  IKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGVETTHQ 124
           IK+SDQI+ AS+WA+GSK H  L+DDS  Y S I+PP+CTLH IS +M CK  G++  H+
Sbjct: 68  IKSSDQISAASHWAQGSKVHVELSDDSFTYSSSIEPPVCTLHDISKQMGCKAAGIDIAHK 127

Query: 125 TTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVALLRKE 184
           TTLDIL+KL +Y W+AKAVLIFTAFA NYG LWHLDNYS SDPLAKSLA IKRV  LRKE
Sbjct: 128 TTLDILSKLTKYSWEAKAVLIFTAFAMNYGALWHLDNYSRSDPLAKSLAMIKRVGSLRKE 187

Query: 185 LDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVSYWVIH 244
           LDSV+YGQVFF PNS+IY+CLK IKY+NEF+NLSKYD K+VPELSAALR IPLVSYW++H
Sbjct: 188 LDSVQYGQVFFGPNSLIYSCLKAIKYMNEFKNLSKYDIKEVPELSAALRGIPLVSYWIVH 247

Query: 245 TLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELYRWLVD 304
            LVASSI+LHCYLSG +G T KYL++L+EK  S+L+TLE H  FI++QQEEVELYRWLVD
Sbjct: 248 ALVASSIDLHCYLSGTEGQTHKYLSELTEKKRSILVTLEKHRQFIQKQQEEVELYRWLVD 307

Query: 305 QTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDISKED 364
           QTDHFPTD+TLFLSKLI GKHKARPLINCST+LEEYIEDF+KEK LILIVSKRLD+SKED
Sbjct: 308 QTDHFPTDITLFLSKLIHGKHKARPLINCSTRLEEYIEDFLKEKNLILIVSKRLDVSKED 367

Query: 365 LEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQIAGVRF 424
           L+ L+ +Y+EVKK NK E+VW+ VI+D P EGDEEAYE L S MKWY VPF+T++AG+RF
Sbjct: 368 LDSLNVVYSEVKKGNKHEIVWVSVISDPPAEGDEEAYEALISKMKWYAVPFATKVAGLRF 427

Query: 425 LEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWIDSTVV 484
           LEE+WE+RED+L+VVLNTQSK+EF NA+HLTR+WEKEA+PFTY+RA  LL ++WIDSTVV
Sbjct: 428 LEEKWEVREDLLVVVLNTQSKIEFPNAVHLTRIWEKEAIPFTYERANTLLKRSWIDSTVV 487

Query: 485 KFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFELVPVG 544
           KFTDQPRL +L +IN+ER +IFYGGH   WIK+FE++AE +KRDP  R+EGITFELVP+G
Sbjct: 488 KFTDQPRLNSLAMINKERTMIFYGGHTQGWIKRFEDTAEAIKRDPMLREEGITFELVPLG 547

Query: 545 MNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAKGPTVT 604
           +N KGE DPTIM RFW AQRS+FILKHQL GST  EDISRLISYENE GWAII KG TV 
Sbjct: 548 LNQKGEPDPTIMSRFWTAQRSFFILKHQLQGSTETEDISRLISYENEKGWAIITKGQTVV 607

Query: 605 FVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSGWIPLF 664
            VGGGDLI+KA+ EFQ WKKNLRRVGF+GSFKDY+DELT+ SL CTHVNI+G+SGWIPL 
Sbjct: 608 MVGGGDLIIKALEEFQTWKKNLRRVGFSGSFKDYFDELTSKSLECTHVNIVGYSGWIPLT 667

Query: 665 ITCPVCRRYMGSGIRFTCCHGGPDVL 691
           + CPVCR+YMGSGIRFTCCHG P+VL
Sbjct: 668 VMCPVCRQYMGSGIRFTCCHGRPNVL 693

BLAST of Lsi09G012650 vs. NCBI nr
Match: XP_038875655.1 (protein SIEVE ELEMENT OCCLUSION B-like [Benincasa hispida])

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 629/690 (91.16%), Postives = 656/690 (95.07%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILF 60
           MS FRPKTSATALALAL +ETDKEEQSLK+LSDDVITNRIFTINS+DE +KIDIDNYILF
Sbjct: 1   MSVFRPKTSATALALALPRETDKEEQSLKNLSDDVITNRIFTINSEDEPMKIDIDNYILF 60

Query: 61  IESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGVE 120
           I+SVIK+SD+IAVAS+WAKG+KGHF L DDSLKYPSQIDPPIC LHQISTEMTCKDPGVE
Sbjct: 61  IDSVIKSSDKIAVASHWAKGNKGHFALEDDSLKYPSQIDPPICILHQISTEMTCKDPGVE 120

Query: 121 TTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVAL 180
           T HQTTL+ILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSD LAKSLATIKRVAL
Sbjct: 121 TAHQTTLEILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDALAKSLATIKRVAL 180

Query: 181 LRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVSY 240
           LRKELDSVKYGQVFFNPNSMIY  LK IKYINEFR LS+YDTKDVPELSAALRQIPLVSY
Sbjct: 181 LRKELDSVKYGQVFFNPNSMIYKSLKAIKYINEFRKLSRYDTKDVPELSAALRQIPLVSY 240

Query: 241 WVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELYR 300
           WVIHTLVASSIELHCYLSGVQG T KYLNDLSEKVGS ++TLENHL  IREQQEEVELYR
Sbjct: 241 WVIHTLVASSIELHCYLSGVQGQTHKYLNDLSEKVGSTVVTLENHLQIIREQQEEVELYR 300

Query: 301 WLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDI 360
           WLVDQTDHFPTD+TLFLSKLIDGKHKARPLINCSTQLEEYIEDF+KEK LILIVSKRLDI
Sbjct: 301 WLVDQTDHFPTDITLFLSKLIDGKHKARPLINCSTQLEEYIEDFLKEKNLILIVSKRLDI 360

Query: 361 SKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQIA 420
           SKEDLEILHSIYNEVKKENKFEMVWIPVI D P EGDEEAYEYLKSTMKWYIVP  TQIA
Sbjct: 361 SKEDLEILHSIYNEVKKENKFEMVWIPVIPDPPIEGDEEAYEYLKSTMKWYIVPLGTQIA 420

Query: 421 GVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWID 480
           GVRFLEERWELRED+LMVVLNTQSKVEFSNA+HLTRVWEKEALPFTYDRAKALL KNW D
Sbjct: 421 GVRFLEERWELREDLLMVVLNTQSKVEFSNAVHLTRVWEKEALPFTYDRAKALLKKNWTD 480

Query: 481 STVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFEL 540
           STVVKFTDQPRLR+LVVINQERNVIFYGGHN RWIK+FEESAE MKRDPTTRDEGITFEL
Sbjct: 481 STVVKFTDQPRLRSLVVINQERNVIFYGGHNPRWIKRFEESAEAMKRDPTTRDEGITFEL 540

Query: 541 VPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAKG 600
           VPV M NKGELDP IMFRFWMAQRSYFILKHQLHGSTAA+DISRLISYENE GWAIIAKG
Sbjct: 541 VPVAM-NKGELDPEIMFRFWMAQRSYFILKHQLHGSTAADDISRLISYENEDGWAIIAKG 600

Query: 601 PTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSGW 660
            TV FVGGGDLILKAM+E+Q+WKKN+RRVGF+GSFKDY+D+LTATSLHCTHVNIIGFSGW
Sbjct: 601 LTVVFVGGGDLILKAMDEYQLWKKNIRRVGFSGSFKDYFDQLTATSLHCTHVNIIGFSGW 660

Query: 661 IPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           IPL ITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 IPLIITCPVCRRYMGSGIRFTCCHGGPDVL 689

BLAST of Lsi09G012650 vs. NCBI nr
Match: KAA0057516.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucumis melo var. makuwa] >TYK08197.1 protein SIEVE ELEMENT OCCLUSION B-like [Cucumis melo var. makuwa])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 608/691 (87.99%), Postives = 646/691 (93.49%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQ-SLKHLSDDVITNRIFTINSDDETIKIDIDNYIL 60
           MS FRPKTSATALALAL QE DKEE+ SLKHLSDD ITNRIFTINSD ET+KIDID+YIL
Sbjct: 1   MSIFRPKTSATALALALPQEIDKEEEKSLKHLSDDFITNRIFTINSDAETMKIDIDSYIL 60

Query: 61  FIESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGV 120
           FIESVIK+SD+IAVAS+WAKGSKGHF LAD++LKYP+QIDPPICTLHQISTEM CKDPG 
Sbjct: 61  FIESVIKSSDKIAVASHWAKGSKGHFELADETLKYPTQIDPPICTLHQISTEMICKDPGA 120

Query: 121 ETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVA 180
           ET HQTTLDIL KLA+Y WDAKAVLIFTAFAT YGVLWHLDNYSHSD LAKSLATIKRVA
Sbjct: 121 ETAHQTTLDILQKLAKYSWDAKAVLIFTAFATTYGVLWHLDNYSHSDTLAKSLATIKRVA 180

Query: 181 LLRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVS 240
           LLRKELDSVKYGQVFFNPNSMIYNC+K IKYINEFRNLSKYDTKDVPELSAALRQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNPNSMIYNCMKAIKYINEFRNLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELY 300
           YW+IHTLVASSIELHCYLSGVQG T KYLN+LSEK  SVLLTLENHL  IREQ EEVELY
Sbjct: 241 YWIIHTLVASSIELHCYLSGVQGQTHKYLNELSEKSESVLLTLENHLQLIREQIEEVELY 300

Query: 301 RWLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLD 360
           RWLVDQTDHFPTD+TLFLSKLIDGKHKARPLINCSTQLEEYIEDF+KEKKLILIVSKRLD
Sbjct: 301 RWLVDQTDHFPTDITLFLSKLIDGKHKARPLINCSTQLEEYIEDFLKEKKLILIVSKRLD 360

Query: 361 ISKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQI 420
           IS EDLEIL+SIYNEVKKENKFEMVWIPVI D P +GDEE YEYLKS MKWY+VPF+T+I
Sbjct: 361 ISTEDLEILYSIYNEVKKENKFEMVWIPVIPDPPMDGDEEGYEYLKSVMKWYMVPFNTKI 420

Query: 421 AGVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWI 480
           AG+RFLEERWELREDILMVVLNTQSKVEFSN IHL R+WEKEALPFTYDRAKALL KNWI
Sbjct: 421 AGMRFLEERWELREDILMVVLNTQSKVEFSNGIHLARIWEKEALPFTYDRAKALLKKNWI 480

Query: 481 DSTVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFE 540
           DSTVVKFTDQPRLR+LVVINQERNVIFYGGHN RWIKKFEESAE MKRDPTTR+EGITFE
Sbjct: 481 DSTVVKFTDQPRLRSLVVINQERNVIFYGGHNLRWIKKFEESAEVMKRDPTTREEGITFE 540

Query: 541 LVPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAK 600
           L PVG+ NKGE DP +MFRFWMAQRSYFILKHQL GSTA EDISRLISYE E GWAII K
Sbjct: 541 LAPVGL-NKGEQDPALMFRFWMAQRSYFILKHQLQGSTATEDISRLISYETEDGWAIITK 600

Query: 601 GPTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSG 660
           GPTV FV GGDLILKAM+EF +WKKN+RR+GF+GSFK+++DELTATSLHCT+VN+IGFSG
Sbjct: 601 GPTVVFVAGGDLILKAMDEFHLWKKNMRRLGFSGSFKEHFDELTATSLHCTNVNLIGFSG 660

Query: 661 WIPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           WIPLF+TCP+CRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLFLTCPMCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lsi09G012650 vs. NCBI nr
Match: XP_004150408.1 (protein SIEVE ELEMENT OCCLUSION B [Cucumis sativus] >KAE8651826.1 hypothetical protein Csa_006103 [Cucumis sativus])

HSP 1 Score: 1240.3 bits (3208), Expect = 0.0e+00
Identity = 606/691 (87.70%), Postives = 648/691 (93.78%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQ-SLKHLSDDVITNRIFTINSDDETIKIDIDNYIL 60
           MS FRPKTSATALALAL QE +KEE+ +LKHLSDDVITNRIFTINSDDET+KIDID+YIL
Sbjct: 1   MSMFRPKTSATALALALPQEINKEEEKNLKHLSDDVITNRIFTINSDDETMKIDIDSYIL 60

Query: 61  FIESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGV 120
           FIESVIK+SD+IAVAS+WAKGSKG+FVLAD+SLKYP+QIDPPICTLHQISTEMTCKDPGV
Sbjct: 61  FIESVIKSSDKIAVASHWAKGSKGNFVLADESLKYPTQIDPPICTLHQISTEMTCKDPGV 120

Query: 121 ETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVA 180
           ET  QTTL+IL KL RY WDAKAVLIFTAFATNYGVLWHLDNYSHSD LAKSLATIKRV+
Sbjct: 121 ETADQTTLNILRKLTRYSWDAKAVLIFTAFATNYGVLWHLDNYSHSDTLAKSLATIKRVS 180

Query: 181 LLRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVS 240
           LLRKELDSVKYGQVFFN NSMIYNC+K IKYINEFR LSKYDTKDVPELSAALRQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCMKAIKYINEFRTLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELY 300
           YW+IHTLVASSIELHCYLSGVQG   KYLN+L EK  S+LLTLENHL  IREQ EEVELY
Sbjct: 241 YWIIHTLVASSIELHCYLSGVQGQAHKYLNELFEKSESILLTLENHLQLIREQIEEVELY 300

Query: 301 RWLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLD 360
           RWLVDQTDHFPTD+TLFLSKLIDGKHKARPLINCSTQLEEYIEDF+KEKKLILIVSKRLD
Sbjct: 301 RWLVDQTDHFPTDITLFLSKLIDGKHKARPLINCSTQLEEYIEDFLKEKKLILIVSKRLD 360

Query: 361 ISKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQI 420
           IS EDLEIL+SIYNEVKKENKFEMVWIPVI D P +GDEEAYEYL S MKWY+VPF+T+I
Sbjct: 361 ISTEDLEILYSIYNEVKKENKFEMVWIPVIPDPPMDGDEEAYEYLISVMKWYVVPFNTKI 420

Query: 421 AGVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWI 480
           AG+RFLEERWELREDILMVVLNTQSKVEFSNAIHLTR+WEKEALPFTYDRAKALL KNWI
Sbjct: 421 AGMRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRIWEKEALPFTYDRAKALLKKNWI 480

Query: 481 DSTVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFE 540
           +STVVKFTDQPRLR+LVVINQERNVIFYGGHN RWIKKFEESAETMKRDPTTR+EGITFE
Sbjct: 481 ESTVVKFTDQPRLRSLVVINQERNVIFYGGHNPRWIKKFEESAETMKRDPTTREEGITFE 540

Query: 541 LVPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAK 600
           L PVGM NKGE DP I FRFWMAQRSYFILKHQL GSTA EDISRLISYE E GWAII K
Sbjct: 541 LAPVGM-NKGEQDPVITFRFWMAQRSYFILKHQLQGSTATEDISRLISYETEDGWAIITK 600

Query: 601 GPTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSG 660
           GPTV FV GGDLILKAM++F +WKKN+RR+GF+GSFK+++DELTATSLHCT+VN+IG+SG
Sbjct: 601 GPTVVFVAGGDLILKAMDQFNLWKKNMRRLGFSGSFKEHFDELTATSLHCTNVNLIGYSG 660

Query: 661 WIPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           WIPLFITCP+CRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLFITCPMCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lsi09G012650 vs. NCBI nr
Match: XP_023548586.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 578/690 (83.77%), Postives = 641/690 (92.90%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILF 60
           MS  RPK+SA ALALALSQ+TDKEE  LKHLSDDVITNRIF   +D+E  KID+DNY+LF
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGVE 120
           IE+V+K SDQI VAS+WA+GSKGHFVLA+D++ YPS IDPPICTLHQIS+EM CK PGVE
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVAL 180
           T H+TTLDIL+KL RYPW+AKAVLIFTAF TNYGVLWHLDN+SHSDPLAKSLATIKRV L
Sbjct: 121 TVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLATIKRVGL 180

Query: 181 LRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVSY 240
           LRKELDSVKYGQVFFN NSMIYNCLK +KYINEF++LSKYDTKDVPELSAALRQIPLVSY
Sbjct: 181 LRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVSY 240

Query: 241 WVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELYR 300
           W+IH LVASSIELHCYLSGV+G T KYLN+++EKV S++LTLENH+ FIREQQEEVELYR
Sbjct: 241 WIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELYR 300

Query: 301 WLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDI 360
           WLVDQTDHFPTDVTLFL+KLI+GKHKARPLINCSTQLEE+IE+FMKEKKLILIVS+ L+ 
Sbjct: 301 WLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLNF 360

Query: 361 SKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQIA 420
           S+EDLEIL S+Y EVKKENKFEMVWIPVI+D PN+GDEEAYE LKS MKW++VPF T+IA
Sbjct: 361 SREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFDTKIA 420

Query: 421 GVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWID 480
           GVRFLEERWELRED+L+VVL+TQSKVEFSNAIHLTRVWEKEA+PF+YDR KAL+ KNWID
Sbjct: 421 GVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWID 480

Query: 481 STVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFEL 540
           STV+KFTDQPRL++LVVINQERNVIFYGGHN RW+K FE+SAE MKRDP TR+EGITFEL
Sbjct: 481 STVLKFTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFEL 540

Query: 541 VPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAKG 600
           VPVG NNKGE DPT+MF FWMAQRSYFILKHQLHGSTA+EDISRLISYE E GWAII KG
Sbjct: 541 VPVGRNNKGEHDPTVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITKG 600

Query: 601 PTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSGW 660
           PTV F+GGGDLILKA++EFQIWKKNLRRVGF+GS+KDY+DELTA+SLHCTHVNIIGFSGW
Sbjct: 601 PTVVFIGGGDLILKAIDEFQIWKKNLRRVGFSGSYKDYFDELTASSLHCTHVNIIGFSGW 660

Query: 661 IPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           IPL ITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 IPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lsi09G012650 vs. NCBI nr
Match: KAG7013505.1 (Protein SIEVE ELEMENT OCCLUSION B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 576/690 (83.48%), Postives = 641/690 (92.90%), Query Frame = 0

Query: 1   MSAFRPKTSATALALALSQETDKEEQSLKHLSDDVITNRIFTINSDDETIKIDIDNYILF 60
           MS  RPK+SATALALALSQ+TDKEE  LKHLSDDVITNRIF   +D+E  KID+DNY+LF
Sbjct: 1   MSLLRPKSSATALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTSDQIAVASNWAKGSKGHFVLADDSLKYPSQIDPPICTLHQISTEMTCKDPGVE 120
           IE+V+K SDQI VAS+WA+GSKGHFVLA+D++ YPS IDPPICTLHQIS+EM CK PGVE
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVAL 180
           T H+TTL+IL+KL RYPW+AKAVLIFTAF TNYGVLWHLDN+SHSDPLAKSLATIKRVAL
Sbjct: 121 TVHKTTLNILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHSDPLAKSLATIKRVAL 180

Query: 181 LRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTKDVPELSAALRQIPLVSY 240
           LRKELDSVKYGQVFFN NSMIYNCLK +KYINEF++LSKYDTKDVPELSAALRQIPLVSY
Sbjct: 181 LRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVSY 240

Query: 241 WVIHTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLLTLENHLHFIREQQEEVELYR 300
           W+IH LVASSIELHCYLSGV+G T KYLN+++EKV S++LTLENH+ FIREQQEEVELYR
Sbjct: 241 WIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELYR 300

Query: 301 WLVDQTDHFPTDVTLFLSKLIDGKHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDI 360
           WLVDQTDHFPTDVTLFL+KLI+GKHKARPLINCSTQLEE+IE+FMKEKKLILIVS+ L+ 
Sbjct: 301 WLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLNF 360

Query: 361 SKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKSTMKWYIVPFSTQIA 420
           S+EDLEIL S+Y EVKKENKFEMVWIPVI+D PN+GDEEAYE LKS MKW++VPF+ +IA
Sbjct: 361 SREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKIA 420

Query: 421 GVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWID 480
           GVRFLEERWELRED+L+VVL+TQSKVEFSNAIHLTRVWEKEA+PF+YDR KAL+ KNWID
Sbjct: 421 GVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWID 480

Query: 481 STVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFEL 540
           STV+K+TDQPRL++LVVINQERNVIFYGGHN RW+K FE+SAE MKRDP TR+EGITFEL
Sbjct: 481 STVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFEL 540

Query: 541 VPVGMNNKGELDPTIMFRFWMAQRSYFILKHQLHGSTAAEDISRLISYENEYGWAIIAKG 600
           VPVG NNKGE DP +MF FWMAQRSYFILKHQLHGSTA+EDISRLISYE E GWAII KG
Sbjct: 541 VPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITKG 600

Query: 601 PTVTFVGGGDLILKAMNEFQIWKKNLRRVGFTGSFKDYYDELTATSLHCTHVNIIGFSGW 660
           PTV F+GGGDLILKA++EFQIWKKNLRRVGF+GSFKDY+DELTA+ LHCTHVNIIGFSGW
Sbjct: 601 PTVVFIGGGDLILKAIDEFQIWKKNLRRVGFSGSFKDYFDELTASGLHCTHVNIIGFSGW 660

Query: 661 IPLFITCPVCRRYMGSGIRFTCCHGGPDVL 691
           IPL ITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 IPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lsi09G012650 vs. TAIR 10
Match: AT3G01680.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01670.1); Has 122 Blast hits to 112 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 219.5 bits (558), Expect = 8.1e-57
Identity = 177/647 (27.36%), Postives = 289/647 (44.67%), Query Frame = 0

Query: 96  SQIDPPICTLHQISTEMTCKDPGVETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGV 155
           S +D     + +++ E+  K      +H+ T+ +   L+ + WD K VL   AFA NYG 
Sbjct: 101 SVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQWDGKLVLTLAAFALNYGE 160

Query: 156 LWHLDNYSHSDPLAKSLATIKRVALL-RKELDSVKYGQVFFNPNSMIYNCLKVIKYINEF 215
            W L  +   + LAKSLA +K V +  R  L+SV  G      N +I     V   + E 
Sbjct: 161 FWLLVQFYSKNQLAKSLAMLKLVPVQNRVTLESVSQGL-----NDLIREMKSVTACVVEL 220

Query: 216 RNL-SKYDTKDVPELSAALRQIPLVSYWVIHTLVASSIELHCYLSGVQGHTLKYLN-DLS 275
             L  +Y T DVP+LS  L  IP+  YW I +++A   +++  +    GH +     DL 
Sbjct: 221 SELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQIN--MITAMGHEMMNTQMDLW 280

Query: 276 E--KVGSVLLTLENHL-HFIR------EQQEEVELYRWLVDQTDHFPTDVTLFLSKLIDG 335
           E   + + L  + +HL   +R      E+Q   E  + L    D    D    L+ L+  
Sbjct: 281 ETSMLANKLKNIHDHLAETLRLCYRHIEKQRSSESLKVLHSLFDTTHIDNMKILTALVHP 340

Query: 336 KHKARPLINCSTQLEEYIEDFMKEKKLILIVSKRLDISKEDLEILHSIYNEV-------- 395
           K    PL +  T+ + ++ D ++ K ++L++S  L+I +++L I   IY E         
Sbjct: 341 KPHITPLQDGLTKRKVHL-DVLRRKTVLLLISD-LNILQDELSIFEQIYTESRRNLVGVD 400

Query: 396 -KKENKFEMVWIPVITDGPNEGDEEA------YEYLKSTMKWYIV--PFSTQIAGVRFLE 455
            K    +E+VW+PV+   P E  E +      +E L+  M WY V  P   +   V F+ 
Sbjct: 401 GKSHMPYEVVWVPVV--DPIEDFERSPILQKKFEDLRDPMPWYSVDSPKLIERHVVEFMR 460

Query: 456 ERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPFTYDRAKALLNKNWIDSTVVKF 515
            RW      ++VV++ Q      NA+H+  +W  EA PFT  R + L  +      ++  
Sbjct: 461 GRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRRETFSLNLI-- 520

Query: 516 TDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAETMKRDPTTRDEGITFELVPVGMN 575
            D         I  +  +  YGG +  WI++F     TM    T +D  +  E+  VG  
Sbjct: 521 VDGIDSVIFNWIKPDNYIFLYGGDDLDWIRRF-----TMAAKATAKDSNVNLEMAYVGKR 580

Query: 576 NKGE--------------------LDPTIMFRFWMAQRSYFILKHQLHGSTAAED----I 635
           N                        +P +M+ FW    S    K QL  +   +D    I
Sbjct: 581 NHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHDDVMQGI 640

Query: 636 SRLISYENEYGWAIIAKGPTVTFVGGGDLILKAMNEF-QIWKKNLRRVGFTGSFKDYYDE 685
            +++SY+   GWA+++KGP +  +  G  I + M+ + + WK ++   G+T +  D++ +
Sbjct: 641 KKILSYDKLGGWALLSKGPEIVMIAHG-AIERTMSVYDRTWKTHVPTKGYTKAMSDHHHD 700

BLAST of Lsi09G012650 vs. TAIR 10
Match: AT3G01670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 121 Blast hits to 111 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 121; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 169.1 bits (427), Expect = 1.3e-41
Identity = 186/733 (25.38%), Postives = 311/733 (42.43%), Query Frame = 0

Query: 31  LSDD-VITNRIFTINSDDETIKIDIDNYILFIESVIKTSDQIAVASNWAKGSKGHFVLAD 90
           LSDD V+ +R+   +S D  I  D+ + +  +  + K+     V S  +   K   V  D
Sbjct: 136 LSDDRVMADRVLKTHSPD-MIFFDVTSLLSVVNDIFKSH----VPSIDSSAPKPSLVFKD 195

Query: 91  --DSLKYPSQIDPPICTLHQISTEMTCK------DPGVETT-------HQTTLDILNKLA 150
             D   + +  D     + QIS E+ CK        G+ T+       + TT  +L+ ++
Sbjct: 196 YADHTSFETFAD----LIDQISCEIDCKCLHGGESHGMMTSGLHLDSRNTTTFSVLSLVS 255

Query: 151 RYPWDAKAVLIFTAFATNYGVLWHLDNYSHSDPLAKSLATIKRVALLRKELDSVKYGQVF 210
           +Y WDAK VL+ +A A  YGV   L     ++ L KSLA IK++              +F
Sbjct: 256 KYRWDAKLVLVLSALAVKYGVFLLLAETHATNQLTKSLALIKQLP------------SIF 315

Query: 211 FNPNSMIYNCLKVIKYINEFRNLSK--YDTKDVP--ELSAALR-QIPLVSYWVI------ 270
              N++     K    + +  +L+    D   +P   ++AA    IP   YW++      
Sbjct: 316 SRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPPNHITAAFTDHIPTAVYWIVRCVLIC 375

Query: 271 --HTLVASSIELHCYLSGVQGHTLKYLNDLSEKVGSVLL--------TLENHLHFIREQQ 330
             H   AS  +    +S ++   +   ++   K+ + LL        T+E  +    E Q
Sbjct: 376 VSHISGASGFKQDQIMSFMEVSEIHENSERLRKINAYLLEQFKKSKMTIEEGI-IEEEYQ 435

Query: 331 EEVELYRWLVDQTDHFPTDVTLFLSKLID------GKHKARPLINCSTQLEEYIEDFMKE 390
           E ++ +  ++   D  P    L L + ID      G  K R  IN  TQ           
Sbjct: 436 ELIQTFTTII-HVDVVPP--LLRLLRPIDFLYHGAGVSKRRVGINVLTQ----------- 495

Query: 391 KKLILIVSKRLDISKEDLEILHSIYNEVKKENKFEMVWIPVITDGPNEGDEEAYEYLKST 450
           K ++L++S   +I KE L IL S+Y E  +++ FE++W+PV  D   E D+  +E L   
Sbjct: 496 KHVLLLISDLENIEKE-LYILESLYTEAWQQS-FEILWVPV-QDFWTEADDAKFEALHMN 555

Query: 451 MKWYIV--PFSTQIAGVRFLEERWELREDILMVVLNTQSKVEFSNAIHLTRVWEKEALPF 510
           M+WY++  P   + A +RF+ E W  +   ++V L+ + +V  +NA  +  +W+  A PF
Sbjct: 556 MRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVALDPKGQVMSTNAFPMVWIWQPFAHPF 615

Query: 511 TYDRAKALLN-KNWIDSTVVKFTDQPRLRTLVVINQERNVIFYGGHNSRWIKKFEESAET 570
           T  R + L + + W    ++  TD   L  LV     + +  YGG + +WIK F     T
Sbjct: 616 TTARERDLWSEQEWNLEFLIDGTDPHSLNQLV---DGKYICLYGGEDMQWIKNF-----T 675

Query: 571 MKRDPTTRDEGITFELVPVGMNN-KGELDPTI-----------------MFRFWMAQRSY 630
                  +   I  E+V VG  N K  + P I                 ++ FW    S 
Sbjct: 676 SLWRNVAKAANIQLEMVYVGKRNPKNGIQPIINTIREENLSHTLPDLFQIWFFWTRVESM 735

Query: 631 FILKHQL---HGSTAAE------------DISRLISYENE-YGWAIIAKGPTVTFVGGGD 684
           +  K ++   HG    E            ++  ++ Y  E  GW +++K   +     G+
Sbjct: 736 WESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAMLGYGGEGDGWGLVSKASDMMVRAKGN 795

BLAST of Lsi09G012650 vs. TAIR 10
Match: AT1G67790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 208 Blast hits to 125 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 1.3e-06
Identity = 40/140 (28.57%), Postives = 64/140 (45.71%), Query Frame = 0

Query: 105 LHQISTEMTCKDPGVETTHQTTLDILNKLARYPWDAKAVLIFTAFATNYGVLWHLDNYSH 164
           + +IS +M C   G     + T+ + + L  Y WDAKAVL+    A  YG L    + + 
Sbjct: 77  IFRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAVLVLGVLAATYGGLLLPVHLAI 136

Query: 165 SDPLAKSLATIKRVALLRKELDSVKYGQVFFNPNSMIYNCLKVIKYINEFRNLSKYDTK- 224
            DP+A S+A + ++ + R      K+     + N +I   + V K I +F  +     K 
Sbjct: 137 CDPVAASIAKLNQLPIER-----TKFRPWLESLNLLIKAMVDVTKCIIKFEKIPFKQAKL 196

Query: 225 DVPELSAALRQIPLVSYWVI 244
           D   L   L  I L +Y V+
Sbjct: 197 DNNILGETLSNIYLTTYRVV 211