Lsi09G012170 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G012170
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	splicing factor U2af large subunit B-like
Location	chr09: 19593606 .. 19596123 (+)
RNA-Seq Expression	Lsi09G012170
Synteny	Lsi09G012170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATCGTTCCACGACTACGAGTTACTACAGAGTAAAATTGCAGAGGGAAAAAAAAAGAAAACCCTCAATTTAGGTTTTTTTTCCTCCTCCTTCATCGCCATCGCCATTCCCATCTTCTCTCTCTTTCTCTGCAGGTTGCCTCAGACACTGCAACGCCGCTCTGCCTCATCGCCGTTGCATTCCAACTCTCATTTCTGCCTTCTATCCAAGGTTACACAGCCACATCTTCTCCCTCATCGCTTTTTTTTATTCTTCTGTTGCTGCTGTTAATTTCATCCTTCCGAGCCCTCATTGACTCTCTAATTGTGGAGAATAGCGTTACATGGGAGATTATTCATTATTAGTGCTTTGTACGTTTTTTTTTTTTTTTTTTTTTTAAAATTTCTTTAGGCTAATTAGAGGTTTTGGGGTTGCAGCATGCTAGTTAACTAGCAAAAGAAAAAAGAAAAAAGGGGACAATTCTTCTTTAAGTGGTTCAAGCTTTGGGTTATGTCTTATTGTAGTCAACTTGAGTAATAAGCTATTAGGAAGTGGGAAAATAAGAGGCTCGATGTAAACCGTTCTCTTGAAGTAAATTTAGTCTGCATTTTAGCTTGCATGGTTAGGTTTTGACTCATTCAATGTTTGGTTTTGGTAATTGATGTTATCTTTAGTTTTCTTCTTAATATGCGTATTTATTGTGTTGTAGGATTATTGAATGTGCAAGTGACTGCGTAGCACTGAGGATTTCCACCTTGACCAGTTATTGATCTTTTAACATGGTCAATCCGGTCTTTGTTTGAGCATTAATTGTCACATTATATCGTTCGTTTAAGTAGGGACCGATGAGCACGCATAGTTGCTCAAAACAATACAGTAGAAGGAGTAAAAAGCAGACCCAGAGCAACTCTAATGATGAAAGTGCAGCTCGGACTAGGCCACTCAGCTTTGAAGATATTATGCTTAGAAGGAAGAACAAAGAGTCAGCTGCTACGGTTGAGGTGGGTGCCAGTGCCACTGGTAGCCATTCAAGAAGGGAAAGCATAGATAAGCACAGTACTGATAATCGTGCATCTGAAAGGCATTTTCATCACAGTAAAGGTTCTTCCCTTGATGTGCAAAATCTTTCATTTGAGGAATCAGCCAAAGATAGTTCAAGAAGGAAAAAAGAGGAGACATTGTTGAAGGACAATATGGTGGTTAGGAGTGATAGAAATAGTTATGAATCTGGATTGTCACTGATGAGTAAGCTTAAGTACGACAGGAATGGAAAGGATAAAAGACAGAAATATGGCCACGAAAATCTTGGTTGGGGAAAAAATGACCAGAGTTCTAGAATTGATATTGAAACTGAAACTGGAAAGAGGCATTCAAGAGATACAGCTGGCAAGGATAGACGTGAAGATCATGGTAGGGGGAAATTTGAAAGAGAAAGTAAGAGAAAATCTCAAAATGGTGATGATGACAGGAATAGAGACAAGTATATTGCAAAGAGACATGATCGTGGTAAACATCATGACCTAGAAAATAGAGAAAGAAAGGAAGCTAAAGTATCATCGACTTCATATTATGAAGATTCTAGATTGAAAAGAAGACGAAAAAGAAGCCCAGATCGTGAAAGTAAACATAGAAGATCTGTTTCACTTTCTCCAAGGCCACACAAGCACTCAACTAAGTTAGATAGGCAGAAGGAGTTGCCATTAGAGTCTCATGTAAAGAAGTCTGGAAGATGGCGTTCTGATAGTGATAGAACAGGGGATTTTACCAATACTTCCAATAGCCAATACCGGCGACATTCTGGGTCAACAAGTGGGCTTGGTGGCTATTCACCTAGAAAGAGAAGAACTGAATCTGCTGTCAAGACTCCTTCACCTGTTCGATCACCAGAGAAGAAAAATGAAGTGTTGGATCTTCCTCCAACAGAAAAGGTTGGATTATTTTCTGGCTCAGTTACTTCCAACTTCCAGCTGCCAAATCCTACTGTTTCCTTGGGCATTAGTAATGATCAATCTGGTGGTGCACTCTTTTCTTCTGCTATGGGGAAATCTTTATCAGGGGTTTCTTCAAATAATATAGCAATGAAGACAAAGGTTTCTCTTGATTTGATTCAGCTGACCCAAGCTACTCGGCCAATGAGGAGGCTTTATATTGAAAACTTACCACATTCTGCATCTGAGAAAGCAATCATTGATTGCCTGAATGGTTTTCTTACGTCTTCAGGTGTTAATCACATCGAAGGAACCCAGCCATGTATTAGTTGTATTGTAAGTGCTTTATTCTATGTTCTAAATGTGTGTCGCTATTTTGTTATGATATTGTCTGATGGGAGTTTTGGTGCACAACACCACTTGTGCAGATACACAAAGATAGGGGGCAAGCTCTTGTTGAATTTCTTACTCCTGAGGATGCTTCAGCAGCTCTTTCGTTTGATGGAAGTGACTTCTATGGCTCTATTCTAAAGATTCGGCGACCAAAAGATTATATTGAAATTGTAGTAAGAATAAATATCCAGAATTCTTGGCCATCTCCATTCTCTTGA

mRNA sequence

AAATCGTTCCACGACTACGAGTTACTACAGAGTAAAATTGCAGAGGGAAAAAAAAAGAAAACCCTCAATTTAGGTTTTTTTTCCTCCTCCTTCATCGCCATCGCCATTCCCATCTTCTCTCTCTTTCTCTGCAGGTTGCCTCAGACACTGCAACGCCGCTCTGCCTCATCGCCGTTGCATTCCAACTCTCATTTCTGCCTTCTATCCAAGGATTATTGAATGTGCAAGTGACTGCGTAGCACTGAGGATTTCCACCTTGACCAGTTATTGATCTTTTAACATGGTCAATCCGGTCTTTGTTTGAGCATTAATTGTCACATTATATCGTTCGTTTAAGTAGGGACCGATGAGCACGCATAGTTGCTCAAAACAATACAGTAGAAGGAGTAAAAAGCAGACCCAGAGCAACTCTAATGATGAAAGTGCAGCTCGGACTAGGCCACTCAGCTTTGAAGATATTATGCTTAGAAGGAAGAACAAAGAGTCAGCTGCTACGGTTGAGGTGGGTGCCAGTGCCACTGGTAGCCATTCAAGAAGGGAAAGCATAGATAAGCACAGTACTGATAATCGTGCATCTGAAAGGCATTTTCATCACAGTAAAGGTTCTTCCCTTGATGTGCAAAATCTTTCATTTGAGGAATCAGCCAAAGATAGTTCAAGAAGGAAAAAAGAGGAGACATTGTTGAAGGACAATATGGTGGTTAGGAGTGATAGAAATAGTTATGAATCTGGATTGTCACTGATGAGTAAGCTTAAGTACGACAGGAATGGAAAGGATAAAAGACAGAAATATGGCCACGAAAATCTTGGTTGGGGAAAAAATGACCAGAGTTCTAGAATTGATATTGAAACTGAAACTGGAAAGAGGCATTCAAGAGATACAGCTGGCAAGGATAGACGTGAAGATCATGGTAGGGGGAAATTTGAAAGAGAAAGTAAGAGAAAATCTCAAAATGGTGATGATGACAGGAATAGAGACAAGTATATTGCAAAGAGACATGATCGTGGTAAACATCATGACCTAGAAAATAGAGAAAGAAAGGAAGCTAAAGTATCATCGACTTCATATTATGAAGATTCTAGATTGAAAAGAAGACGAAAAAGAAGCCCAGATCGTGAAAGTAAACATAGAAGATCTGTTTCACTTTCTCCAAGGCCACACAAGCACTCAACTAAGTTAGATAGGCAGAAGGAGTTGCCATTAGAGTCTCATGTAAAGAAGTCTGGAAGATGGCGTTCTGATAGTGATAGAACAGGGGATTTTACCAATACTTCCAATAGCCAATACCGGCGACATTCTGGGTCAACAAGTGGGCTTGGTGGCTATTCACCTAGAAAGAGAAGAACTGAATCTGCTGTCAAGACTCCTTCACCTGTTCGATCACCAGAGAAGAAAAATGAAGTGTTGGATCTTCCTCCAACAGAAAAGGTTGGATTATTTTCTGGCTCAGTTACTTCCAACTTCCAGCTGCCAAATCCTACTGTTTCCTTGGGCATTAGTAATGATCAATCTGGTGGTGCACTCTTTTCTTCTGCTATGGGGAAATCTTTATCAGGGGTTTCTTCAAATAATATAGCAATGAAGACAAAGGTTTCTCTTGATTTGATTCAGCTGACCCAAGCTACTCGGCCAATGAGGAGGCTTTATATTGAAAACTTACCACATTCTGCATCTGAGAAAGCAATCATTGATTGCCTGAATGGTTTTCTTACGTCTTCAGGTGTTAATCACATCGAAGGAACCCAGCCATGTATTAGTTGTATTATACACAAAGATAGGGGGCAAGCTCTTGTTGAATTTCTTACTCCTGAGGATGCTTCAGCAGCTCTTTCGTTTGATGGAAGTGACTTCTATGGCTCTATTCTAAAGATTCGGCGACCAAAAGATTATATTGAAATTGTAGTAAGAATAAATATCCAGAATTCTTGGCCATCTCCATTCTCTTGA

Coding sequence (CDS)

ATGAGCACGCATAGTTGCTCAAAACAATACAGTAGAAGGAGTAAAAAGCAGACCCAGAGCAACTCTAATGATGAAAGTGCAGCTCGGACTAGGCCACTCAGCTTTGAAGATATTATGCTTAGAAGGAAGAACAAAGAGTCAGCTGCTACGGTTGAGGTGGGTGCCAGTGCCACTGGTAGCCATTCAAGAAGGGAAAGCATAGATAAGCACAGTACTGATAATCGTGCATCTGAAAGGCATTTTCATCACAGTAAAGGTTCTTCCCTTGATGTGCAAAATCTTTCATTTGAGGAATCAGCCAAAGATAGTTCAAGAAGGAAAAAAGAGGAGACATTGTTGAAGGACAATATGGTGGTTAGGAGTGATAGAAATAGTTATGAATCTGGATTGTCACTGATGAGTAAGCTTAAGTACGACAGGAATGGAAAGGATAAAAGACAGAAATATGGCCACGAAAATCTTGGTTGGGGAAAAAATGACCAGAGTTCTAGAATTGATATTGAAACTGAAACTGGAAAGAGGCATTCAAGAGATACAGCTGGCAAGGATAGACGTGAAGATCATGGTAGGGGGAAATTTGAAAGAGAAAGTAAGAGAAAATCTCAAAATGGTGATGATGACAGGAATAGAGACAAGTATATTGCAAAGAGACATGATCGTGGTAAACATCATGACCTAGAAAATAGAGAAAGAAAGGAAGCTAAAGTATCATCGACTTCATATTATGAAGATTCTAGATTGAAAAGAAGACGAAAAAGAAGCCCAGATCGTGAAAGTAAACATAGAAGATCTGTTTCACTTTCTCCAAGGCCACACAAGCACTCAACTAAGTTAGATAGGCAGAAGGAGTTGCCATTAGAGTCTCATGTAAAGAAGTCTGGAAGATGGCGTTCTGATAGTGATAGAACAGGGGATTTTACCAATACTTCCAATAGCCAATACCGGCGACATTCTGGGTCAACAAGTGGGCTTGGTGGCTATTCACCTAGAAAGAGAAGAACTGAATCTGCTGTCAAGACTCCTTCACCTGTTCGATCACCAGAGAAGAAAAATGAAGTGTTGGATCTTCCTCCAACAGAAAAGGTTGGATTATTTTCTGGCTCAGTTACTTCCAACTTCCAGCTGCCAAATCCTACTGTTTCCTTGGGCATTAGTAATGATCAATCTGGTGGTGCACTCTTTTCTTCTGCTATGGGGAAATCTTTATCAGGGGTTTCTTCAAATAATATAGCAATGAAGACAAAGGTTTCTCTTGATTTGATTCAGCTGACCCAAGCTACTCGGCCAATGAGGAGGCTTTATATTGAAAACTTACCACATTCTGCATCTGAGAAAGCAATCATTGATTGCCTGAATGGTTTTCTTACGTCTTCAGGTGTTAATCACATCGAAGGAACCCAGCCATGTATTAGTTGTATTATACACAAAGATAGGGGGCAAGCTCTTGTTGAATTTCTTACTCCTGAGGATGCTTCAGCAGCTCTTTCGTTTGATGGAAGTGACTTCTATGGCTCTATTCTAAAGATTCGGCGACCAAAAGATTATATTGAAATTGTAGTAAGAATAAATATCCAGAATTCTTGGCCATCTCCATTCTCTTGA

Protein sequence

MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGSHSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVRSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIVVRINIQNSWPSPFS

Homology

[Close]

BLAST of Lsi09G012170 vs. ExPASy Swiss-Prot
Match: P90978 (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 PE=2 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 8.1e-09
Identity = 78/295 (26.44%), Postives = 126/295 (42.71%), Query Frame = 0


Query: 226 LENRERKEAKVSSTSYYEDS-RLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKEL 285
           LEN E  ++        +D+ R KR+R RS DR+++ R            S   DR +  
Sbjct: 37  LENEENLKSSTGGGGGEDDNDRKKRKRSRSRDRDTRRR------------SRSRDRGE-- 96

Query: 286 PLESHVKKSGRWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKT---P 345
                 ++ G    D DR       S S+ RR  G     G   PR+R  +   ++   P
Sbjct: 97  ------RRGGGGGGDRDR-------SRSRERRRGGG----GRDEPRRRGGDDEARSRREP 156

Query: 346 SPVRSPE-KKNEVLDLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGK 405
            P +  E KK    D+PPT             F+   P   +   N Q+ G +   ++  
Sbjct: 157 EPQKPREPKKYRFWDVPPT------------GFETTTP---MEYKNMQAAGQVPRGSVQS 216

Query: 406 SLSGVSSNNIAMKTKVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGV 465
           ++  V  +                  T   RRLY+ N+P   +E+A++D  N  +   G+
Sbjct: 217 AVPVVGPS-----------------VTCQSRRLYVGNIPFGCNEEAMLDFFNQQMHLCGL 267

Query: 466 NHIEGTQPCISCIIHKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
               G  P + C I+ D+  A +EF + ++ +A ++FDG +F G  LK+RRP+DY
Sbjct: 277 AQAPG-NPILLCQINLDKNFAFIEFRSIDETTAGMAFDGINFMGQQLKVRRPRDY 267

[Close]

BLAST of Lsi09G012170 vs. ExPASy Swiss-Prot
Match: O23212 (Splicing factor U2af large subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF65A PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 3.1e-08
Identity = 85/341 (24.93%), Postives = 149/341 (43.70%), Query Frame = 0


Query: 175 HSRDTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEA 234
           +  +  G+D     G  + + +SK K ++ D +R   +   +  ++G+  D E    +++
Sbjct: 18  YDEENGGRD-----GEIEDQLDSKPKRESRDHERETSRSKDREREKGRDKDRE----RDS 77

Query: 235 KVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSG 294
           +VS  S   D    + R R  DR+  HR     S R   HS +   ++E        + G
Sbjct: 78  EVSRRSRDRDGEKSKERSRDKDRD--HRERHHRSSRHRDHSRERGERRE--------RGG 137

Query: 295 RWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVL 354
           R   D  R+ D     + + R   G        S  K R+E   ++ SP +S ++ +   
Sbjct: 138 RDDDDYRRSRD---RDHDRRRDDRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSG-F 197

Query: 355 DLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT 414
           D+ P     L +G+  +    P P    G     +   +F    G+S  G+S   I   T
Sbjct: 198 DMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPLPTGQSFGGLSMMPIQAMT 257

Query: 415 KVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCII 474
           +         QATR  RR+Y+  L  +A+E+++    +  + + G N        ++  I
Sbjct: 258 Q---------QATRHARRVYVGGLSPTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYI 317

Query: 475 HKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
           + ++  A VE  + E+AS A+S DG  F G+ +K+RRP DY
Sbjct: 318 NHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY 323

[Close]

BLAST of Lsi09G012170 vs. ExPASy Swiss-Prot
Match: P26368 (Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4)

HSP 1 Score: 58.5 bits (140), Expect = 2.6e-07
Identity = 71/277 (25.63%), Postives = 125/277 (45.13%), Query Frame = 0


Query: 239 TSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRS 298
           + + E  R     K+  D+E++HR+      R H  S   DR++            R RS
Sbjct: 2   SDFDEFERQLNENKQERDKENRHRK------RSHSRSRSRDRKR------------RSRS 61

Query: 299 DSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPP 358
              R  D  + S  + RR    T G       K      +++P   +  +K  +  D+PP
Sbjct: 62  RDRRNRDQRSASRDRRRRSKPLTRG------AKEEHGGLIRSPRHEKK-KKVRKYWDVPP 121

Query: 359 TEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSL 418
                         F+   P   +     Q+ G + ++A+   L  ++ + +A+ T   +
Sbjct: 122 ------------PGFEHITP---MQYKAMQAAGQIPATAL---LPTMTPDGLAV-TPTPV 181

Query: 419 DLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDR 478
            ++  +Q TR  RRLY+ N+P   +E+A++D  N  +   G+    G  P ++  I++D+
Sbjct: 182 PVVG-SQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPG-NPVLAVQINQDK 232

Query: 479 GQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
             A +EF + ++ + A++FDG  F G  LKIRRP DY
Sbjct: 242 NFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDY 232

[Close]

BLAST of Lsi09G012170 vs. ExPASy Swiss-Prot
Match: P26369 (Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3)

HSP 1 Score: 58.5 bits (140), Expect = 2.6e-07
Identity = 71/277 (25.63%), Postives = 125/277 (45.13%), Query Frame = 0


Query: 239 TSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRS 298
           + + E  R     K+  D+E++HR+      R H  S   DR++            R RS
Sbjct: 2   SDFDEFERQLNENKQERDKENRHRK------RSHSRSRSRDRKR------------RSRS 61

Query: 299 DSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPP 358
              R  D  + S  + RR    T G       K      +++P   +  +K  +  D+PP
Sbjct: 62  RDRRNRDQRSASRDRRRRSKPLTRG------AKEEHGGLIRSPRHEKK-KKVRKYWDVPP 121

Query: 359 TEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSL 418
                         F+   P   +     Q+ G + ++A+   L  ++ + +A+ T   +
Sbjct: 122 ------------PGFEHITP---MQYKAMQAAGQIPATAL---LPTMTPDGLAV-TPTPV 181

Query: 419 DLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDR 478
            ++  +Q TR  RRLY+ N+P   +E+A++D  N  +   G+    G  P ++  I++D+
Sbjct: 182 PVVG-SQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPG-NPVLAVQINQDK 232

Query: 479 GQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
             A +EF + ++ + A++FDG  F G  LKIRRP DY
Sbjct: 242 NFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDY 232

[Close]

BLAST of Lsi09G012170 vs. ExPASy Swiss-Prot
Match: Q8L716 (Splicing factor U2af large subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF65B PE=1 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 3.4e-07
Identity = 103/401 (25.69%), Postives = 166/401 (41.40%), Query Frame = 0


Query: 118 VVRSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSR 177
           V  S  N  E+ +SL S   ++    D + +  H+     K D S   + + E G R   
Sbjct: 11  VAISTENHNENYISLESSPFHE----DSKSRESHDL----KKDSSKISEKDNENG-RDKD 70

Query: 178 DTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAK-RHDRGKHHDLENRERKEAKV 237
               +DR +D  R K     + KS++ D DR R K   + RH R +H D  +RER E + 
Sbjct: 71  GNKDRDREKDRDREKSRDRDREKSRDRDRDRERSKDRQRDRHHRDRHRD-RSRERSEKRD 130

Query: 238 S-STSYYEDSRLK-RRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSG 297
                ++  SR + RRR R  DRE +HRR                           +   
Sbjct: 131 DLDDDHHRRSRDRDRRRSRDRDREVRHRR---------------------------RSRS 190

Query: 298 RWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVL 357
           R RS S+R     +   S++R  S S S                      RS  K+    
Sbjct: 191 RSRSRSERRSRSEHRHKSEHRSRSRSRS----------------------RSKSKRRSGF 250

Query: 358 DLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT 417
           D+ P +   L + +V +  Q+P+   +  I    S   +F    G+ L  +    +   T
Sbjct: 251 DMAPPDM--LAATAVAAAGQVPSVPTTATIPGMFSN--MFPMVPGQQLGALPVLPVQAMT 310

Query: 418 KVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCII 477
           +         QATR  RR+Y+  LP +A+E+++    +  +++ G N        ++  I
Sbjct: 311 Q---------QATRHARRVYVGGLPPTANEQSVSTFFSQVMSAIGGNTAGPGDAVVNVYI 339

Query: 478 HKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
           + ++  A VE  + E+AS A++ DG    G  +K+RRP DY
Sbjct: 371 NHEKKFAFVEMRSVEEASNAMALDGIILEGVPVKVRRPTDY 339

[Close]

BLAST of Lsi09G012170 vs. ExPASy TrEMBL
Match: A0A6J1H6S0 (splicing factor U2af large subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111460135 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.6e-225
Identity = 435/519 (83.82%), Postives = 463/519 (89.21%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MS++S SK YSRRSKKQT SNSNDESAARTRPLSFEDIMLRRKNK SA TVEVG   TGS
Sbjct: 1   MSSYSFSKHYSRRSKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVG--VTGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
           HSRRESIDK STDN ASERHF H KG+SLDVQNLS EES KDSSRRKKEETLLKDNM VR
Sbjct: 61  HSRRESIDKLSTDNCASERHFRHGKGASLDVQNLSLEESGKDSSRRKKEETLLKDNMEVR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+Y+S L+LM KLKYD NG DK+QKYG EN+G GK +QSSR+DIE ETGKRHSRD+ 
Sbjct: 121 SDRNNYDSELTLMGKLKYDANGNDKKQKYGQENVGRGKKNQSSRVDIEKETGKRHSRDS- 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
            K +RED GRG FER  KRK QNGDD+ NRDKY AKRHD GKHHD ENRERKEAK S TS
Sbjct: 181 -KAKREDLGRGNFERGGKRKDQNGDDEGNRDKYAAKRHDHGKHHDPENRERKEAKASLTS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
            YEDSRL+RRRKRS DRESKHRRSVSLSPRPHKHS+KL RQKELPL+SHVKKSGRWRSDS
Sbjct: 241 NYEDSRLQRRRKRSQDRESKHRRSVSLSPRPHKHSSKLVRQKELPLDSHVKKSGRWRSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTE 360
           DRTGD TN+SNSQYRRH GSTSGLGGYSPRKRRTESA+KTPSPV+SPEKK+E LD+PPTE
Sbjct: 301 DRTGDLTNSSNSQYRRHFGSTSGLGGYSPRKRRTESAIKTPSPVQSPEKKDEGLDVPPTE 360

Query: 361 KVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDL 420
           K+GLFS S+ SNFQ  N TVS GI N QSGGA+FSS +GKSL+GVSSNNI MKT VSLDL
Sbjct: 361 KIGLFSSSINSNFQPSNATVSSGIINVQSGGAIFSSVIGKSLTGVSSNNIEMKTNVSLDL 420

Query: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ 480
           IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHI+GT PCISCIIHKDRGQ
Sbjct: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIKGTLPCISCIIHKDRGQ 480

Query: 481 ALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           ALVEFLTPEDASAALSFDGSDF GSILKIRRPKDY+EIV
Sbjct: 481 ALVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIV 515

[Close]

BLAST of Lsi09G012170 vs. ExPASy TrEMBL
Match: A0A1S3CCY4 (splicing factor U2af large subunit B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499435 PE=4 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.8e-224
Identity = 453/610 (74.26%), Postives = 474/610 (77.70%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MSTHSCSKQYSRRSKKQT+SNS+DE+AARTRP SFEDIMLRRK K S+ATVEV       
Sbjct: 1   MSTHSCSKQYSRRSKKQTRSNSDDETAARTRPFSFEDIMLRRKTKGSSATVEV------- 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSF-EESAKDSSRRKKEETLLKDNMVV 120
                  D  STDNRASERHF HSKGSSLDVQN+S  EESAKDSSRRKKEET+LK+N+VV
Sbjct: 61  -------DVTSTDNRASERHFRHSKGSSLDVQNVSLEEESAKDSSRRKKEETVLKNNIVV 120

Query: 121 RSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDT 180
           R+DRN+YESGLSLMSKLK+DRN KD+R+KYG ENLGWGKNDQS RIDIETET KRHSRDT
Sbjct: 121 RNDRNNYESGLSLMSKLKHDRNEKDEREKYGQENLGWGKNDQSCRIDIETETAKRHSRDT 180

Query: 181 AGKDRREDHGRGKFERE------------------------------------------- 240
           A KDRR+DHGRG+FERE                                           
Sbjct: 181 AFKDRRQDHGRGEFERESKRKSQNGDDDRNRDSKRKSQNGDDDRNRDSKRKSQNGDDDRS 240

Query: 241 -----------------------------------------------SKRKSQNGDDDRN 300
                                                          SKRKSQNGDDDRN
Sbjct: 241 RDSKRKSQNGDDDRNRDSKRKSQNGDDERNRDGKRKSQNGDDDRNRDSKRKSQNGDDDRN 300

Query: 301 RDKYIAKRHDRGKHHDLENRERKEAKVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSP 360
           RDKYIAKRHD GKHHDLENRE+KEA VS TS Y+DSRLKR RKRS DRESKHRRSVSLSP
Sbjct: 301 RDKYIAKRHDHGKHHDLENREKKEAIVSLTSRYQDSRLKRTRKRSSDRESKHRRSVSLSP 360

Query: 361 RPHKHSTKLDRQKELPLESHVKKSGRWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSP 420
           R HKHSTKL RQKELPL+SHVKKSGRWRSDS+RTGDFTNTSNSQYRRHSGSTSGLGGYSP
Sbjct: 361 RSHKHSTKLARQKELPLDSHVKKSGRWRSDSERTGDFTNTSNSQYRRHSGSTSGLGGYSP 420

Query: 421 RKRRTESAVKTPSPVRSPEKKNEVLDLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQS 480
           RKRRTESAVKTPSP+ SPEKKNEVLDLPPTEKVGLFSGSV SNF   NPTVSLGISNDQS
Sbjct: 421 RKRRTESAVKTPSPLLSPEKKNEVLDLPPTEKVGLFSGSVASNFSPSNPTVSLGISNDQS 480

Query: 481 GGALFSSAMGKSLSGVSSNNIAMKTKVSLDLIQLTQATRPMRRLYIENLPHSASEKAIID 520
           GGA FSSAMGKSLS VSSNNIAMKTKVS DL+QLTQATRPMRRLYIENLPHSASEKAIID
Sbjct: 481 GGAFFSSAMGKSLSVVSSNNIAMKTKVSFDLVQLTQATRPMRRLYIENLPHSASEKAIID 540

[Close]

BLAST of Lsi09G012170 vs. ExPASy TrEMBL
Match: A0A6J1C9Y6 (splicing factor U2af large subunit A OS=Momordica charantia OX=3673 GN=LOC111009561 PE=4 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.5e-223
Identity = 430/520 (82.69%), Postives = 462/520 (88.85%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MSTHSCSK+YSR S K T  N+NDESAARTRP SFEDIMLRRKNK SAA VE GA+  GS
Sbjct: 1   MSTHSCSKKYSRSSVKHTLYNTNDESAARTRPFSFEDIMLRRKNKGSAANVEGGATVAGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
            SRR+SID   TDNR SER F HS+GSSLDVQNL  EESAK SSRRKKEETLLKD+ VVR
Sbjct: 61  RSRRDSIDNDITDNRESERLFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDSTVVR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+YESGL+LM K+KY+ NGKDK QKYG ENLGWGK+DQ +RIDIE E+GKRHSRD A
Sbjct: 121 SDRNNYESGLTLMGKVKYETNGKDKGQKYGQENLGWGKSDQRTRIDIENESGKRHSRDLA 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
           GKDRRED GRGKFERESKRK QNGDDD+NRD+Y  K+HD  KH DLENR+RKEAKVS +S
Sbjct: 181 GKDRREDRGRGKFERESKRKYQNGDDDKNRDRYSEKKHDHSKHRDLENRDRKEAKVSLSS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
           +YEDSR KRRRKRS DRESKHRRSVSLSPR HKHSTKL R KELP +SH+KKSGRWRSDS
Sbjct: 241 HYEDSRFKRRRKRSQDRESKHRRSVSLSPRAHKHSTKLVRHKELP-DSHLKKSGRWRSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTE 360
           DRTGDFTN+S SQYRRHSGSTSGLGGYSPRKRRTESA+KTPSPVRSPEKKNE LDL P +
Sbjct: 301 DRTGDFTNSSTSQYRRHSGSTSGLGGYSPRKRRTESAIKTPSPVRSPEKKNEALDLLPAD 360

Query: 361 KVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT-KVSLD 420
           KVGLFSGSVTS FQ  NPTVS  IS DQS GALFSSAMGKSLSGVSSNN+ MKT  VSLD
Sbjct: 361 KVGLFSGSVTSIFQPSNPTVSSVISTDQSSGALFSSAMGKSLSGVSSNNLPMKTANVSLD 420

Query: 421 LIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRG 480
           LIQLTQATRPMRRLYIEN+PHSASEKA+I+C+NGFLTSSGVNHIEGTQPCISCIIHKDRG
Sbjct: 421 LIQLTQATRPMRRLYIENVPHSASEKAVIECMNGFLTSSGVNHIEGTQPCISCIIHKDRG 480

Query: 481 QALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           QALVEFLTPEDASAALSFDG++F GSILKIRRPKDY+EIV
Sbjct: 481 QALVEFLTPEDASAALSFDGTNFSGSILKIRRPKDYVEIV 519

[Close]

BLAST of Lsi09G012170 vs. ExPASy TrEMBL
Match: A0A1S3CD15 (splicing factor U2af large subunit B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499435 PE=4 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 9.6e-223
Identity = 453/625 (72.48%), Postives = 474/625 (75.84%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MSTHSCSKQYSRRSKKQT+SNS+DE+AARTRP SFEDIMLRRK K S+ATVEV       
Sbjct: 1   MSTHSCSKQYSRRSKKQTRSNSDDETAARTRPFSFEDIMLRRKTKGSSATVEV------- 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSF-EESAKDSSRRKKEETLLKDNMVV 120
                  D  STDNRASERHF HSKGSSLDVQN+S  EESAKDSSRRKKEET+LK+N+VV
Sbjct: 61  -------DVTSTDNRASERHFRHSKGSSLDVQNVSLEEESAKDSSRRKKEETVLKNNIVV 120

Query: 121 RSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDT 180
           R+DRN+YESGLSLMSKLK+DRN KD+R+KYG ENLGWGKNDQS RIDIETET KRHSRDT
Sbjct: 121 RNDRNNYESGLSLMSKLKHDRNEKDEREKYGQENLGWGKNDQSCRIDIETETAKRHSRDT 180

Query: 181 AGKDRREDHGRGKFERE------------------------------------------- 240
           A KDRR+DHGRG+FERE                                           
Sbjct: 181 AFKDRRQDHGRGEFERESKRKSQNGDDDRNRDSKRKSQNCVDESNKDGRRKSQNGDDDRN 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 RDSKRKSQNGDDDRSRDSKRKSQNGDDDRNRDSKRKSQNGDDERNRDGKRKSQNGDDDRN 300

Query: 301 --SKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTSYYEDSRLKRRRKRS 360
             SKRKSQNGDDDRNRDKYIAKRHD GKHHDLENRE+KEA VS TS Y+DSRLKR RKRS
Sbjct: 301 RDSKRKSQNGDDDRNRDKYIAKRHDHGKHHDLENREKKEAIVSLTSRYQDSRLKRTRKRS 360

Query: 361 PDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDSDRTGDFTNTSNSQY 420
            DRESKHRRSVSLSPR HKHSTKL RQKELPL+SHVKKSGRWRSDS+RTGDFTNTSNSQY
Sbjct: 361 SDRESKHRRSVSLSPRSHKHSTKLARQKELPLDSHVKKSGRWRSDSERTGDFTNTSNSQY 420

Query: 421 RRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTEKVGLFSGSVTSNFQ 480
           RRHSGSTSGLGGYSPRKRRTESAVKTPSP+ SPEKKNEVLDLPPTEKVGLFSGSV SNF 
Sbjct: 421 RRHSGSTSGLGGYSPRKRRTESAVKTPSPLLSPEKKNEVLDLPPTEKVGLFSGSVASNFS 480

Query: 481 LPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDLIQLTQATRPMRRLY 520
             NPTVSLGISNDQSGGA FSSAMGKSLS VSSNNIAMKTKVS DL+QLTQATRPMRRLY
Sbjct: 481 PSNPTVSLGISNDQSGGAFFSSAMGKSLSVVSSNNIAMKTKVSFDLVQLTQATRPMRRLY 540

[Close]

BLAST of Lsi09G012170 vs. ExPASy TrEMBL
Match: A0A6J1HJZ8 (splicing factor U2af large subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC111464254 PE=4 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 4.0e-221
Identity = 428/520 (82.31%), Postives = 460/520 (88.46%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MST+SCSKQYSR S KQ+  NSNDESAARTRP SFEDIMLRRKNKESAA        TGS
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN---DGGDTGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
           HSRRESIDKH T N  SERHF HS+GSSLD+QNL  EESAK SSRRKKEETLLKDNMV+R
Sbjct: 61  HSRRESIDKHITANLESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+YESGL+L+ KLK D NGKDKRQKYG ENLGWGKND+ SRIDIE ETGKR SRD A
Sbjct: 121 SDRNNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRRSRDAA 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
           GKDRRED G+GK ERESKRK Q+GDDDRNRD++I K+HD GKHHDLE++ERKEAK+S +S
Sbjct: 181 GKDRREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
           ++EDSRLK+RRKRS D +SKH++S SLSPRP K STKL RQKELPL+SHVKKSGRW SDS
Sbjct: 241 HHEDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGST-SGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPT 360
           DR GDFTN+S+SQYRRHSGST SGLGGYSPRKRRTESAVKTP PVRSPEKKNE LDL P 
Sbjct: 301 DRIGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPA 360

Query: 361 EKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLD 420
           E VGLFSGSVTSNFQ  NPTVS GISND SGGALFS AMGKSLSGVSSNN+A KT  S+D
Sbjct: 361 ETVGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASID 420

Query: 421 LIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRG 480
            IQLTQATRPMRRLYIENLPHS SEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRG
Sbjct: 421 SIQLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRG 480

Query: 481 QALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           QALVEFLTPEDASAALSFDGSDF GSIL+IRRPKDY+E+V
Sbjct: 481 QALVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV 516

[Close]

BLAST of Lsi09G012170 vs. NCBI nr
Match: XP_038906743.1 (splicing factor U2af large subunit B [Benincasa hispida])

HSP 1 Score: 912.5 bits (2357), Expect = 1.7e-261
Identity = 493/519 (94.99%), Postives = 501/519 (96.53%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MST+SCSKQYSRRSKKQT SNSNDESAARTRPLSFEDIMLRRKNK SAATVEVG  ATGS
Sbjct: 1   MSTYSCSKQYSRRSKKQTPSNSNDESAARTRPLSFEDIMLRRKNKGSAATVEVG--ATGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
           HSRRESIDKHS DNRASERH  HSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR
Sbjct: 61  HSRRESIDKHSIDNRASERHVRHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+YESGLSLMSKLKYD+NGKDKRQKYG ENLGW K DQSSRIDI+TETGKRHSRDTA
Sbjct: 121 SDRNNYESGLSLMSKLKYDKNGKDKRQKYGQENLGWEKIDQSSRIDIDTETGKRHSRDTA 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
           GKDRREDH RGKFERESKRKSQNGDDDRNRDKY AKRHD GKHHDLENRERKEAKVS TS
Sbjct: 181 GKDRREDHDRGKFERESKRKSQNGDDDRNRDKYNAKRHDHGKHHDLENRERKEAKVSLTS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
           +YEDSRLKRRRKRSPDRES+HRRSVSLSPRPHKHSTKL RQKELPLESHVKKSGRWRSDS
Sbjct: 241 HYEDSRLKRRRKRSPDRESRHRRSVSLSPRPHKHSTKLTRQKELPLESHVKKSGRWRSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTE 360
           DRTGDF+NTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPV+SPEKKNEVLDLPPTE
Sbjct: 301 DRTGDFSNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVQSPEKKNEVLDLPPTE 360

Query: 361 KVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDL 420
           KVGLFSGSVTSNFQ PNPTVSLGISNDQSGG LFSSAMGKSLSGVSSNNIAMKTKVSLDL
Sbjct: 361 KVGLFSGSVTSNFQPPNPTVSLGISNDQSGGTLFSSAMGKSLSGVSSNNIAMKTKVSLDL 420

Query: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ 480
           IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ
Sbjct: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ 480

Query: 481 ALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           ALVEFLTPEDASAALSFDGSDF GSILKIRRPKDYIEIV
Sbjct: 481 ALVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYIEIV 517

[Close]

BLAST of Lsi09G012170 vs. NCBI nr
Match: XP_022959019.1 (splicing factor U2af large subunit B-like [Cucurbita moschata])

HSP 1 Score: 790.4 bits (2040), Expect = 9.5e-225
Identity = 435/519 (83.82%), Postives = 463/519 (89.21%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MS++S SK YSRRSKKQT SNSNDESAARTRPLSFEDIMLRRKNK SA TVEVG   TGS
Sbjct: 1   MSSYSFSKHYSRRSKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVG--VTGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
           HSRRESIDK STDN ASERHF H KG+SLDVQNLS EES KDSSRRKKEETLLKDNM VR
Sbjct: 61  HSRRESIDKLSTDNCASERHFRHGKGASLDVQNLSLEESGKDSSRRKKEETLLKDNMEVR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+Y+S L+LM KLKYD NG DK+QKYG EN+G GK +QSSR+DIE ETGKRHSRD+ 
Sbjct: 121 SDRNNYDSELTLMGKLKYDANGNDKKQKYGQENVGRGKKNQSSRVDIEKETGKRHSRDS- 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
            K +RED GRG FER  KRK QNGDD+ NRDKY AKRHD GKHHD ENRERKEAK S TS
Sbjct: 181 -KAKREDLGRGNFERGGKRKDQNGDDEGNRDKYAAKRHDHGKHHDPENRERKEAKASLTS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
            YEDSRL+RRRKRS DRESKHRRSVSLSPRPHKHS+KL RQKELPL+SHVKKSGRWRSDS
Sbjct: 241 NYEDSRLQRRRKRSQDRESKHRRSVSLSPRPHKHSSKLVRQKELPLDSHVKKSGRWRSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTE 360
           DRTGD TN+SNSQYRRH GSTSGLGGYSPRKRRTESA+KTPSPV+SPEKK+E LD+PPTE
Sbjct: 301 DRTGDLTNSSNSQYRRHFGSTSGLGGYSPRKRRTESAIKTPSPVQSPEKKDEGLDVPPTE 360

Query: 361 KVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDL 420
           K+GLFS S+ SNFQ  N TVS GI N QSGGA+FSS +GKSL+GVSSNNI MKT VSLDL
Sbjct: 361 KIGLFSSSINSNFQPSNATVSSGIINVQSGGAIFSSVIGKSLTGVSSNNIEMKTNVSLDL 420

Query: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ 480
           IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHI+GT PCISCIIHKDRGQ
Sbjct: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIKGTLPCISCIIHKDRGQ 480

Query: 481 ALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           ALVEFLTPEDASAALSFDGSDF GSILKIRRPKDY+EIV
Sbjct: 481 ALVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIV 515

[Close]

BLAST of Lsi09G012170 vs. NCBI nr
Match: XP_023548141.1 (splicing factor U2af large subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.9 bits (2036), Expect = 2.8e-224
Identity = 436/519 (84.01%), Postives = 463/519 (89.21%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MS++S SK YSRRSKKQT SNSNDESAARTRPLSFEDIMLRRKNK SA TVEVG   TGS
Sbjct: 1   MSSYSFSKHYSRRSKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVG--VTGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
           HSRRESIDK STDN ASERHF H KG+SLDVQNLS EES KDSSRRKKEETLLKDNM VR
Sbjct: 61  HSRRESIDKLSTDNCASERHFRHGKGASLDVQNLSLEESGKDSSRRKKEETLLKDNMEVR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+Y+S L+LM KLKYD NG DK+QKYG EN+G GKN+QSSR+DIE ETGKRHSRD+ 
Sbjct: 121 SDRNNYDSELTLMGKLKYDANGNDKKQKYGQENVGRGKNNQSSRVDIEKETGKRHSRDS- 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
            K +RED  RGKFER  KRK QNGDD+ NRDKY AKRHD  KHHD ENR+RKEAK   TS
Sbjct: 181 -KAKREDLDRGKFERGGKRKDQNGDDEGNRDKYAAKRHDHCKHHDPENRKRKEAKSLLTS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
            YEDSRLKRRRKRS DRESKHRRSVSLSPRPHKHS+KL RQKELPL+SHVKKSGRWRSDS
Sbjct: 241 NYEDSRLKRRRKRSQDRESKHRRSVSLSPRPHKHSSKLVRQKELPLDSHVKKSGRWRSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTE 360
           DRTGD TN+SNSQYRRHSGSTSGLGGYSPRKRRTESA+KTPSPV+SPEKK+E LDLPPTE
Sbjct: 301 DRTGDLTNSSNSQYRRHSGSTSGLGGYSPRKRRTESAIKTPSPVQSPEKKDEGLDLPPTE 360

Query: 361 KVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDL 420
           K+GLFS S+ SNFQ  N TVS GI N QSGGA+FSS +GKSL+GVSSNNI MKT VSLDL
Sbjct: 361 KIGLFSSSINSNFQPSNATVSSGIINVQSGGAIFSSVIGKSLTGVSSNNIEMKTNVSLDL 420

Query: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ 480
           IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHI+GT PCISCIIHKDRGQ
Sbjct: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIKGTLPCISCIIHKDRGQ 480

Query: 481 ALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           ALVEFLTPEDASAALSFDGSDF GSILKIRRPKDY+EIV
Sbjct: 481 ALVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIV 515

[Close]

BLAST of Lsi09G012170 vs. NCBI nr
Match: KAG6574901.1 (Splicing factor U2AF 65 kDa subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 788.5 bits (2035), Expect = 3.6e-224
Identity = 433/519 (83.43%), Postives = 464/519 (89.40%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MS++S SK YSRR+KKQT SNSNDESAARTRPLSFEDIMLRRKNK SA TVEVG   TGS
Sbjct: 1   MSSYSFSKHYSRRNKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVG--VTGS 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVVR 120
           HSRRES+DK STDN ASERHF H KG+SLDVQNLS EES KDSSRRKKEETLLKDNM VR
Sbjct: 61  HSRRESVDKLSTDNCASERHFRHGKGASLDVQNLSLEESGKDSSRRKKEETLLKDNMEVR 120

Query: 121 SDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDTA 180
           SDRN+Y+S L+LM KLKYD NG DK+QKYG EN+G GK +QSSR+DIE ETGKRHSRD+ 
Sbjct: 121 SDRNNYDSELTLMGKLKYDANGNDKKQKYGQENVGRGKKNQSSRVDIEKETGKRHSRDS- 180

Query: 181 GKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEAKVSSTS 240
            K +RED GRG FER  KRK QNGD++ NRDKY AKRHD GKHHD ENRERKEAK S TS
Sbjct: 181 -KAKREDLGRGNFERGGKRKDQNGDNEGNRDKYAAKRHDHGKHHDPENRERKEAKASLTS 240

Query: 241 YYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSGRWRSDS 300
            YEDSRL+RRRKRS DRESKHRRSVSLSPRPHKHS+KL RQKELPL+SHVKKSGRWRSDS
Sbjct: 241 NYEDSRLQRRRKRSQDRESKHRRSVSLSPRPHKHSSKLVRQKELPLDSHVKKSGRWRSDS 300

Query: 301 DRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVLDLPPTE 360
           DRTGD TN+SNSQYRRHSGSTSGLGGYSPRKRRTESA+KTPSPV+SPEKK+E L+LPPTE
Sbjct: 301 DRTGDLTNSSNSQYRRHSGSTSGLGGYSPRKRRTESAIKTPSPVQSPEKKDEGLNLPPTE 360

Query: 361 KVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKTKVSLDL 420
           K+GLFS S+ SNFQ  N TVS GI N QSGGA+FSS +GKSL+GVSSNNI MKT VSLDL
Sbjct: 361 KIGLFSSSINSNFQPSNATVSSGIINVQSGGAIFSSVIGKSLTGVSSNNIEMKTNVSLDL 420

Query: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQ 480
           IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHI+GT PCISCIIHKDRGQ
Sbjct: 421 IQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIKGTLPCISCIIHKDRGQ 480

Query: 481 ALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIV 520
           ALVEFLTPEDASAALSFDGSDF GSILKIRRPKDY+EIV
Sbjct: 481 ALVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIV 515

[Close]

BLAST of Lsi09G012170 vs. NCBI nr
Match: XP_008460665.1 (PREDICTED: splicing factor U2af large subunit B isoform X2 [Cucumis melo])

HSP 1 Score: 788.5 bits (2035), Expect = 3.6e-224
Identity = 453/610 (74.26%), Postives = 474/610 (77.70%), Query Frame = 0


Query: 1   MSTHSCSKQYSRRSKKQTQSNSNDESAARTRPLSFEDIMLRRKNKESAATVEVGASATGS 60
           MSTHSCSKQYSRRSKKQT+SNS+DE+AARTRP SFEDIMLRRK K S+ATVEV       
Sbjct: 1   MSTHSCSKQYSRRSKKQTRSNSDDETAARTRPFSFEDIMLRRKTKGSSATVEV------- 60

Query: 61  HSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSF-EESAKDSSRRKKEETLLKDNMVV 120
                  D  STDNRASERHF HSKGSSLDVQN+S  EESAKDSSRRKKEET+LK+N+VV
Sbjct: 61  -------DVTSTDNRASERHFRHSKGSSLDVQNVSLEEESAKDSSRRKKEETVLKNNIVV 120

Query: 121 RSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSRDT 180
           R+DRN+YESGLSLMSKLK+DRN KD+R+KYG ENLGWGKNDQS RIDIETET KRHSRDT
Sbjct: 121 RNDRNNYESGLSLMSKLKHDRNEKDEREKYGQENLGWGKNDQSCRIDIETETAKRHSRDT 180

Query: 181 AGKDRREDHGRGKFERE------------------------------------------- 240
           A KDRR+DHGRG+FERE                                           
Sbjct: 181 AFKDRRQDHGRGEFERESKRKSQNGDDDRNRDSKRKSQNGDDDRNRDSKRKSQNGDDDRS 240

Query: 241 -----------------------------------------------SKRKSQNGDDDRN 300
                                                          SKRKSQNGDDDRN
Sbjct: 241 RDSKRKSQNGDDDRNRDSKRKSQNGDDERNRDGKRKSQNGDDDRNRDSKRKSQNGDDDRN 300

Query: 301 RDKYIAKRHDRGKHHDLENRERKEAKVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSP 360
           RDKYIAKRHD GKHHDLENRE+KEA VS TS Y+DSRLKR RKRS DRESKHRRSVSLSP
Sbjct: 301 RDKYIAKRHDHGKHHDLENREKKEAIVSLTSRYQDSRLKRTRKRSSDRESKHRRSVSLSP 360

Query: 361 RPHKHSTKLDRQKELPLESHVKKSGRWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSP 420
           R HKHSTKL RQKELPL+SHVKKSGRWRSDS+RTGDFTNTSNSQYRRHSGSTSGLGGYSP
Sbjct: 361 RSHKHSTKLARQKELPLDSHVKKSGRWRSDSERTGDFTNTSNSQYRRHSGSTSGLGGYSP 420

Query: 421 RKRRTESAVKTPSPVRSPEKKNEVLDLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQS 480
           RKRRTESAVKTPSP+ SPEKKNEVLDLPPTEKVGLFSGSV SNF   NPTVSLGISNDQS
Sbjct: 421 RKRRTESAVKTPSPLLSPEKKNEVLDLPPTEKVGLFSGSVASNFSPSNPTVSLGISNDQS 480

Query: 481 GGALFSSAMGKSLSGVSSNNIAMKTKVSLDLIQLTQATRPMRRLYIENLPHSASEKAIID 520
           GGA FSSAMGKSLS VSSNNIAMKTKVS DL+QLTQATRPMRRLYIENLPHSASEKAIID
Sbjct: 481 GGAFFSSAMGKSLSVVSSNNIAMKTKVSFDLVQLTQATRPMRRLYIENLPHSASEKAIID 540

[Close]

BLAST of Lsi09G012170 vs. TAIR 10
Match: AT2G33435.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 220.7 bits (561), Expect = 2.8e-57
Identity = 184/470 (39.15%), Postives = 245/470 (52.13%), Query Frame = 0


Query: 60  SHSRRESIDKHSTDNRASERHFHHSKGSSLDVQNLSFEESAKDSSRRKKEETLLKDNMVV 119
           S SR E++DK   D   S R    ++G     +  S EE +KD  R KKEET    N   
Sbjct: 530 SSSREENLDKQKKDESTSNRK-RKAEGECSTAETESIEEHSKD-RRGKKEET----NSNC 589

Query: 120 RSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETET-------- 179
           R +R                   +DK+ K   E+L   +  +      ET+T        
Sbjct: 590 REER-------------------RDKKMK--KEDLASNRKIEGEIPTTETKTMTDRDGLC 649

Query: 180 GKRHSRDTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRER 239
            K+  R     D   D    K +   KRK+QNG+  +NR+  ++KRHD GK H +E  ER
Sbjct: 650 SKKRLRSLVVADVPRDESSIKPDNGDKRKNQNGNHKKNREINMSKRHDPGKVHSVEVSER 709

Query: 240 KEAKVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVK 299
            E +    S   D R KRRR RS D     ++  S  PR  K +++  R  E   E+ VK
Sbjct: 710 WERREQPKSRQRDLREKRRRSRSRDHGQDRQKRSSPLPRAEKATSRHKRNHEERSENVVK 769

Query: 300 KSGRWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVR-SPEKK 359
                   +D     T+T +++ RR+S S S LGGYSPRKRR +++ K  SP   S EKK
Sbjct: 770 DRSGKHHCNDNEDKVTSTVSNKSRRYSASKSELGGYSPRKRREQASTKAASPPNLSSEKK 829

Query: 360 NEVLDLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNI 419
           +    L  T   G+FS SV S  Q    T    IS  ++   L    M   +        
Sbjct: 830 SAKWGLAATVTAGMFSDSVFSGLQAATQTAYPTIS--EASLTLLKPLM---VMDAPFRTP 889

Query: 420 AMKTKVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCI 479
             +   S D +QLT++TR MRRLY EN+P SASEK++I+C NG++ SSG NHI+G++PCI
Sbjct: 890 PARQTTSFDSVQLTESTRRMRRLYAENVPDSASEKSLIECFNGYMLSSGSNHIKGSEPCI 949

Query: 480 SCIIHKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDYIEIVV 521
           SCII+K++ QALVEFLTP+DASAALS DG  F GS LKIRRPKDY+   V
Sbjct: 950 SCIINKEKSQALVEFLTPQDASAALSLDGCSFAGSNLKIRRPKDYVRTTV 967

[Close]

BLAST of Lsi09G012170 vs. TAIR 10
Match: AT4G36690.1 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-09
Identity = 85/341 (24.93%), Postives = 149/341 (43.70%), Query Frame = 0


Query: 175 HSRDTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEA 234
           +  +  G+D     G  + + +SK K ++ D +R   +   +  ++G+  D E    +++
Sbjct: 18  YDEENGGRD-----GEIEDQLDSKPKRESRDHERETSRSKDREREKGRDKDRE----RDS 77

Query: 235 KVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSG 294
           +VS  S   D    + R R  DR+  HR     S R   HS +   ++E        + G
Sbjct: 78  EVSRRSRDRDGEKSKERSRDKDRD--HRERHHRSSRHRDHSRERGERRE--------RGG 137

Query: 295 RWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVL 354
           R   D  R+ D     + + R   G        S  K R+E   ++ SP +S ++ +   
Sbjct: 138 RDDDDYRRSRD---RDHDRRRDDRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSG-F 197

Query: 355 DLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT 414
           D+ P     L +G+  +    P P    G     +   +F    G+S  G+S   I   T
Sbjct: 198 DMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPLPTGQSFGGLSMMPIQAMT 257

Query: 415 KVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCII 474
           +         QATR  RR+Y+  L  +A+E+++    +  + + G N        ++  I
Sbjct: 258 Q---------QATRHARRVYVGGLSPTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYI 317

Query: 475 HKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
           + ++  A VE  + E+AS A+S DG  F G+ +K+RRP DY
Sbjct: 318 NHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY 323

[Close]

BLAST of Lsi09G012170 vs. TAIR 10
Match: AT4G36690.3 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-09
Identity = 85/341 (24.93%), Postives = 149/341 (43.70%), Query Frame = 0


Query: 175 HSRDTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEA 234
           +  +  G+D     G  + + +SK K ++ D +R   +   +  ++G+  D E    +++
Sbjct: 18  YDEENGGRD-----GEIEDQLDSKPKRESRDHERETSRSKDREREKGRDKDRE----RDS 77

Query: 235 KVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSG 294
           +VS  S   D    + R R  DR+  HR     S R   HS +   ++E        + G
Sbjct: 78  EVSRRSRDRDGEKSKERSRDKDRD--HRERHHRSSRHRDHSRERGERRE--------RGG 137

Query: 295 RWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVL 354
           R   D  R+ D     + + R   G        S  K R+E   ++ SP +S ++ +   
Sbjct: 138 RDDDDYRRSRD---RDHDRRRDDRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSG-F 197

Query: 355 DLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT 414
           D+ P     L +G+  +    P P    G     +   +F    G+S  G+S   I   T
Sbjct: 198 DMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPLPTGQSFGGLSMMPIQAMT 257

Query: 415 KVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCII 474
           +         QATR  RR+Y+  L  +A+E+++    +  + + G N        ++  I
Sbjct: 258 Q---------QATRHARRVYVGGLSPTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYI 317

Query: 475 HKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
           + ++  A VE  + E+AS A+S DG  F G+ +K+RRP DY
Sbjct: 318 NHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY 323

[Close]

BLAST of Lsi09G012170 vs. TAIR 10
Match: AT4G36690.2 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-09
Identity = 85/341 (24.93%), Postives = 149/341 (43.70%), Query Frame = 0


Query: 175 HSRDTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAKRHDRGKHHDLENRERKEA 234
           +  +  G+D     G  + + +SK K ++ D +R   +   +  ++G+  D E    +++
Sbjct: 18  YDEENGGRD-----GEIEDQLDSKPKRESRDHERETSRSKDREREKGRDKDRE----RDS 77

Query: 235 KVSSTSYYEDSRLKRRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSG 294
           +VS  S   D    + R R  DR+  HR     S R   HS +   ++E        + G
Sbjct: 78  EVSRRSRDRDGEKSKERSRDKDRD--HRERHHRSSRHRDHSRERGERRE--------RGG 137

Query: 295 RWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVL 354
           R   D  R+ D     + + R   G        S  K R+E   ++ SP +S ++ +   
Sbjct: 138 RDDDDYRRSRD---RDHDRRRDDRGGRRSRRSRSRSKDRSERRTRSRSPSKSKQRVSG-F 197

Query: 355 DLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT 414
           D+ P     L +G+  +    P P    G     +   +F    G+S  G+S   I   T
Sbjct: 198 DMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPLPTGQSFGGLSMMPIQAMT 257

Query: 415 KVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCII 474
           +         QATR  RR+Y+  L  +A+E+++    +  + + G N        ++  I
Sbjct: 258 Q---------QATRHARRVYVGGLSPTANEQSVATFFSQVMAAVGGNTAGPGDAVVNVYI 317

Query: 475 HKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
           + ++  A VE  + E+AS A+S DG  F G+ +K+RRP DY
Sbjct: 318 NHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY 323

[Close]

BLAST of Lsi09G012170 vs. TAIR 10
Match: AT1G60900.1 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 58.2 bits (139), Expect = 2.4e-08
Identity = 103/401 (25.69%), Postives = 166/401 (41.40%), Query Frame = 0


Query: 118 VVRSDRNSYESGLSLMSKLKYDRNGKDKRQKYGHENLGWGKNDQSSRIDIETETGKRHSR 177
           V  S  N  E+ +SL S   ++    D + +  H+     K D S   + + E G R   
Sbjct: 11  VAISTENHNENYISLESSPFHE----DSKSRESHDL----KKDSSKISEKDNENG-RDKD 70

Query: 178 DTAGKDRREDHGRGKFERESKRKSQNGDDDRNRDKYIAK-RHDRGKHHDLENRERKEAKV 237
               +DR +D  R K     + KS++ D DR R K   + RH R +H D  +RER E + 
Sbjct: 71  GNKDRDREKDRDREKSRDRDREKSRDRDRDRERSKDRQRDRHHRDRHRD-RSRERSEKRD 130

Query: 238 S-STSYYEDSRLK-RRRKRSPDRESKHRRSVSLSPRPHKHSTKLDRQKELPLESHVKKSG 297
                ++  SR + RRR R  DRE +HRR                           +   
Sbjct: 131 DLDDDHHRRSRDRDRRRSRDRDREVRHRR---------------------------RSRS 190

Query: 298 RWRSDSDRTGDFTNTSNSQYRRHSGSTSGLGGYSPRKRRTESAVKTPSPVRSPEKKNEVL 357
           R RS S+R     +   S++R  S S S                      RS  K+    
Sbjct: 191 RSRSRSERRSRSEHRHKSEHRSRSRSRS----------------------RSKSKRRSGF 250

Query: 358 DLPPTEKVGLFSGSVTSNFQLPNPTVSLGISNDQSGGALFSSAMGKSLSGVSSNNIAMKT 417
           D+ P +   L + +V +  Q+P+   +  I    S   +F    G+ L  +    +   T
Sbjct: 251 DMAPPDM--LAATAVAAAGQVPSVPTTATIPGMFSN--MFPMVPGQQLGALPVLPVQAMT 310

Query: 418 KVSLDLIQLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIEGTQPCISCII 477
           +         QATR  RR+Y+  LP +A+E+++    +  +++ G N        ++  I
Sbjct: 311 Q---------QATRHARRVYVGGLPPTANEQSVSTFFSQVMSAIGGNTAGPGDAVVNVYI 339

Query: 478 HKDRGQALVEFLTPEDASAALSFDGSDFYGSILKIRRPKDY 516
           + ++  A VE  + E+AS A++ DG    G  +K+RRP DY
Sbjct: 371 NHEKKFAFVEMRSVEEASNAMALDGIILEGVPVKVRRPTDY 339

The following BLAST results are available for this feature:

BLAST of Lsi09G012170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P90978	8.1e-09	26.44	Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 P...	[more]
O23212	3.1e-08	24.93	Splicing factor U2af large subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF65A ...	[more]
P26368	2.6e-07	25.63	Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4	[more]
P26369	2.6e-07	25.63	Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3	[more]
Q8L716	3.4e-07	25.69	Splicing factor U2af large subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF65B ...	[more]

BLAST of Lsi09G012170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6S0	4.6e-225	83.82	splicing factor U2af large subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A1S3CCY4	1.8e-224	74.26	splicing factor U2af large subunit B isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1C9Y6	1.5e-223	82.69	splicing factor U2af large subunit A OS=Momordica charantia OX=3673 GN=LOC111009...	[more]
A0A1S3CD15	9.6e-223	72.48	splicing factor U2af large subunit B isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A6J1HJZ8	4.0e-221	82.31	splicing factor U2af large subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Lsi09G012170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906743.1	1.7e-261	94.99	splicing factor U2af large subunit B [Benincasa hispida]	[more]
XP_022959019.1	9.5e-225	83.82	splicing factor U2af large subunit B-like [Cucurbita moschata]	[more]
XP_023548141.1	2.8e-224	84.01	splicing factor U2af large subunit B-like [Cucurbita pepo subsp. pepo]	[more]
KAG6574901.1	3.6e-224	83.43	Splicing factor U2AF 65 kDa subunit, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_008460665.1	3.6e-224	74.26	PREDICTED: splicing factor U2af large subunit B isoform X2 [Cucumis melo]	[more]

BLAST of Lsi09G012170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33435.1	2.8e-57	39.15	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT4G36690.1	2.2e-09	24.93	U2 snRNP auxilliary factor, large subunit, splicing factor	[more]
AT4G36690.3	2.2e-09	24.93	U2 snRNP auxilliary factor, large subunit, splicing factor	[more]
AT4G36690.2	2.2e-09	24.93	U2 snRNP auxilliary factor, large subunit, splicing factor	[more]
AT1G60900.1	2.4e-08	25.69	U2 snRNP auxilliary factor, large subunit, splicing factor	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 432..510 e-value: 2.0E-7 score: 40.6
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 431..514 score: 10.888906
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 426..526 e-value: 7.2E-20 score: 73.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 99..121
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 163..251
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..351
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 272..302
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 137..153
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 303..327
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..86
None	No IPR available	PANTHER	PTHR23139	RNA-BINDING PROTEIN	coord: 1..519
None	No IPR available	PANTHER	PTHR23139:SF56	RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN	coord: 1..519
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 418..515

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G012170.1	Lsi09G012170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding