Lsi09G011450 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G011450
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionubiquitin-conjugating enzyme E2 E2-like
Locationchr09: 18356277 .. 18360965 (-)
RNA-Seq ExpressionLsi09G011450
SyntenyLsi09G011450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAAGTCAAGTAAACGCTCAATAATTTTTTTTATTTCATTCCATTTCTTCACTCTTCTTCTCAAATCCTTCCTTCACTCTTCCGATCCAATTTCCTTCTTTCCGCCATGCTTCTTTCCCGTCTCCGCCGTTGCTTCTCTTCAAAACCCTCCTCTTCCTCCGCCTACCGCCGCCTCTACACTCCTCCTCGTCCGATCGCCGACACACTCTGTCCCCGCCCAAATCCTTCCCCGCCGCCCTCTCCTCTTCCAATCTACGACGATGATCGTAACCGGCCAGAGTCTCCGATCGGCGACGGCGGAAGCGGAATTGAAGAAAAAACTAGTGCGATGTATCGGATTGAGAACAAAGAGGAGGTAGCAACGGACTGCAGCTTCGGGCCGGTTGCCGCCGATATTAGCGGAAATGAAGAAAAAACTAGTGCGATGTATCGGATTGAGAAGGAGGAGGAGGTGGCGACGGACTGCAGCTTCGGGCCGGTTGCCGCCGATATTAGCGGAAATGAAAAAAAAACTAGTGCGATGTATCGGATTGAGAACGAGTTGAGAGTGATGAACGATGAGGCGGCGTCGCACTGCAGTTTCGGGCCGGTGGGCGGCGATATTTTCCGGTGGGAAGGGGTTGTGATTGGGCCGGCCTGTTCTTGCTATGAAGGGGGTATTTTTCATCTTTCAATTCAATTTCCCTCCGACTACCCCTTATATCCTCCCTACGTCAAGTTTCTAACCAAGGTTAGTTATGTCCATTTTCCTCATTTTATTAATAATATTATTATTAATTTTCTTTTAACTGTTTGAGAATTGCATTTTGGAAAGCCTGACCTATTCTGAAATTGTAAATTTGTAATTGTATGTATGCCTAAATTATAGGTATTTTGGTAATATCTTATAACATTTTAAGAAAGGTTAAAAAATGTTTTTACTAGAGGTGATGTAATTTAGGTTTTTGAACTGACTGATTTATTAATAATAAGAAATTAAATCGATGTCCAATAAATGATAAAACCAACGATTTGATTTTTTAAAAAAGTTTTGTTCCATGTCGGTTTGGTTTTTGATTTTAGATTCAATTAATTTTTTTTCTCAACTTTTATTTTGAATTGGATTAAAATGGGGTGGGTGGTTGTTTAATAAAGTTTCATCATATATGACAACATACTAAAAGTTTCAAATAATACATGGTTCATTTTAAGGTTTATATATACTATCAATTTAATTTTGGTGAGTATTTCAATAAAAAAATAAAGCCAAACCAATAATTTTTTTATTTACACTAAATACAAACCAATGTCCAATTTAGTAGTGGAGGCTTGGATGGATTTGATTTTTCTAGTTTTTCAAGTTTTTTTTTTTTGTTGCACTCCTAATGAAAACTATTAGTATATTATTTAAAAAATACCCGTAAAATATTTTATCTATACTACAAAATACTCTTGAAAAAGTTGCATTAATATTCTTAATATTTTTTTAAAGAATAAATAAAAAGTTATACTAGTGTTAGTATTTGGATGGAAACCATTTATTTACACTTTATCACCTCTCTCTTTCTTTCTTCTCTCTCCTCTACTTCAAACTATGGTCTAACACATTTTCACATAGATACATCATATTTTCTCCCATTTGTTCAATTCTTACACTAATCTCAACAACCAAACTAGACCAAAAATTTAAAGTGAAAATATAAAATCCCACAAAATTCTACGGAGTCCAAAAATAAATAACTCCCATCAAAACTAAGCAAAGCAATGAATCACATTATGGAAAACATTCTTAATTGATCACAATAAATAGACTAATTGGAATGCAAAATGGATCAACAAAATCTTCATCGCTACAAATTTGGTAATGCTCATGTTTTCTTTCTCTTCACTCCTAGTTGTCTTCAAAACTATGATACCAATTTTTAGCTTGTCTCTCATCTGATGTATTAAATGTCATATTAACTCATACTTAATTACTAAGATACAATTTTGTTAAAACATTTAAGTTTTAAGGTTTTTTGGTGGGTTACCCGTTTTATTATTCTCTTTTCTTCTTGTTATTGCCAATATACGTTGATTGAAGGGGTAGTTTTAGATATAGGAGAATGGAGGAAGGGGAAAAAGAGGGTGTCAAAGAAGAGTGGAAAAAACAGAGGAAAAGTTGGAAGTGTCATTGAAACAGAAAAAAGAGAGTGAAATGAGAGGGAGAGCTGAGGGTGATAAATGATTTCCATCCATATGGTAATAGTAACATTTTTCCTTTTTCTTTTTTCCTTTTTTTAAGAATAAGACTTTTAATATCATTTTTGAAAGTTTAAGGATATTTTTCAAATCTATCCATATATTAACGGTAAGAATATTTATTTATTTTTTTAAGAAAATTAAGAGTATTATTGAAACTTTTGAAAATTTAGGGATATTTTTTAAATAAATTAGTAATGTGAGTAGTTCTATCTAAAATAAGTAGTAAGAATATTTTTAATTTTTTTTTTAAATTGTTATTAAAACTTTTGAAAGTTTATGAGTATTATCGTGTTCAAAAATATTTTTTTATACCTTAGCCAAAAGTATATCTTTTCATGGTAGGTTAAGTTGAGGATTTTATCTAATTTCTATTATGTTAGTAGATTTTGTTATCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAACAATGCACTCTCAACGTGTTGTAAATATATTTTTCTAAATAACTTCTCGAAAAATTTAAATGGTACCTTCATTTATTAATTTTTGTAACAATGTTTAAAGAGAGAATAAAATACAAGTATTTGCAACCGTGATAAATATTTCTCTAAACGTTATAAACCATTGATTAATATAATTAGGGTATTGTCATATTTTATTTTAAAGTGTTAATTGATATAATTATTAGTTTAAAATTTTAATTTACTCATAACCGACAAGGTTTAAGGGGAATAAATCGATTTCCCCCTAAAATATTTTAAGTCTGTTTTTCTTTGTTCTTCGTTTTTCTTCTGTTTACGCCTCTATTTCTTCATTTTTCTTCCGTTTACATCTCTATTTCGTAGAAGGCATTTTGATGTGTAAGACTTAGATTTTAAAATTTTAAAATAAGATGATTGAGTTTAAAGTATAAAACCATGAATGTTTTGATGTATTATGTATTTTTATTTTAGTCGGTTACTGATTCTTTCAGTTTAGTTAGTTACTTAGTTGGTTCTTTATTTAATCCTTGTAATTCTTTAGTTCTTTAGTTACGGTTAGTAACTAATTCTTTTAGGTTATTACTCTAAAGTTTAGTATTTAAACTAAACTTCTCTTTCATTTTGAATACAATTTGAAGTATTTCAGAATTTAGACTTGTCTTTCAACTTAGTGTTCCTATATTAACAGACATATTGCAAAGTTTTTTTACACATATTTAATTTGTATACGTAAGAGATTTGTGTTTAATTCTATCTCTGATTGTCTCTACTAAAAAGAAGTTCCATGTTATGTGTGTTGATTGAAACAGATATTCCATCCAAATGTAGGGTTAGATGGAAGCATTTACTTGGACATATTGAATGAAAATTGGTCTTCAGTATTGACTATTGAGAAAGTTCTAATTTCAATATGCTCAGTCCTTTCAAATCCAATTCCTCCAACCTCCATTAATGAAGCTTCAAGAATGTACCTCAATAATTGGCTCAATTTCAATGGGATTGCAAGAGAATGGACCAAAATATATGCAATGCCAAATTGATCTCATCACTAAACAAACACCATCATATTGTAAATAGTATGTATTGAATGATATCATTCAATTGGTTTTAGGGCTCCTAAAAAGGTTGGATTTTGATCTTGGTGAATGTTTTCTGTTATTTTTGCCAAGCCCAACATTGTATTTGATTGTATAATGATCTTAGTTTGTGTAATTATTTATAGTTTCTAATTATAACGAGTATATTCAGTTGAATGATAATTAATT

mRNA sequence

GTCAAGTCAAGTAAACGCTCAATAATTTTTTTTATTTCATTCCATTTCTTCACTCTTCTTCTCAAATCCTTCCTTCACTCTTCCGATCCAATTTCCTTCTTTCCGCCATGCTTCTTTCCCGTCTCCGCCGTTGCTTCTCTTCAAAACCCTCCTCTTCCTCCGCCTACCGCCGCCTCTACACTCCTCCTCGTCCGATCGCCGACACACTCTGTCCCCGCCCAAATCCTTCCCCGCCGCCCTCTCCTCTTCCAATCTACGACGATGATCGTAACCGGCCAGAGTCTCCGATCGGCGACGGCGGAAGCGGAATTGAAGAAAAAACTAGTGCGATGTATCGGATTGAGAACAAAGAGGAGGTAGCAACGGACTGCAGCTTCGGGCCGGTTGCCGCCGATATTAGCGGAAATGAAGAAAAAACTAGTGCGATGTATCGGATTGAGAAGGAGGAGGAGGTGGCGACGGACTGCAGCTTCGGGCCGGTTGCCGCCGATATTAGCGGAAATGAAAAAAAAACTAGTGCGATGTATCGGATTGAGAACGAGTTGAGAGTGATGAACGATGAGGCGGCGTCGCACTGCAGTTTCGGGCCGGTGGGCGGCGATATTTTCCGGTGGGAAGGGGTTGTGATTGGGCCGGCCTGTTCTTGCTATGAAGGGGGTATTTTTCATCTTTCAATTCAATTTCCCTCCGACTACCCCTTATATCCTCCCTACGTCAAGTTTCTAACCAAGATATTCCATCCAAATGTAGGGTTAGATGGAAGCATTTACTTGGACATATTGAATGAAAATTGGTCTTCAGTATTGACTATTGAGAAAGTTCTAATTTCAATATGCTCAGTCCTTTCAAATCCAATTCCTCCAACCTCCATTAATGAAGCTTCAAGAATGTACCTCAATAATTGGCTCAATTTCAATGGGATTGCAAGAGAATGGACCAAAATATATGCAATGCCAAATTGATCTCATCACTAAACAAACACCATCATATTGTAAATAGTATGTATTGAATGATATCATTCAATTGGTTTTAGGGCTCCTAAAAAGGTTGGATTTTGATCTTGGTGAATGTTTTCTGTTATTTTTGCCAAGCCCAACATTGTATTTGATTGTATAATGATCTTAGTTTGTGTAATTATTTATAGTTTCTAATTATAACGAGTATATTCAGTTGAATGATAATTAATT

Coding sequence (CDS)

ATGCTTCTTTCCCGTCTCCGCCGTTGCTTCTCTTCAAAACCCTCCTCTTCCTCCGCCTACCGCCGCCTCTACACTCCTCCTCGTCCGATCGCCGACACACTCTGTCCCCGCCCAAATCCTTCCCCGCCGCCCTCTCCTCTTCCAATCTACGACGATGATCGTAACCGGCCAGAGTCTCCGATCGGCGACGGCGGAAGCGGAATTGAAGAAAAAACTAGTGCGATGTATCGGATTGAGAACAAAGAGGAGGTAGCAACGGACTGCAGCTTCGGGCCGGTTGCCGCCGATATTAGCGGAAATGAAGAAAAAACTAGTGCGATGTATCGGATTGAGAAGGAGGAGGAGGTGGCGACGGACTGCAGCTTCGGGCCGGTTGCCGCCGATATTAGCGGAAATGAAAAAAAAACTAGTGCGATGTATCGGATTGAGAACGAGTTGAGAGTGATGAACGATGAGGCGGCGTCGCACTGCAGTTTCGGGCCGGTGGGCGGCGATATTTTCCGGTGGGAAGGGGTTGTGATTGGGCCGGCCTGTTCTTGCTATGAAGGGGGTATTTTTCATCTTTCAATTCAATTTCCCTCCGACTACCCCTTATATCCTCCCTACGTCAAGTTTCTAACCAAGATATTCCATCCAAATGTAGGGTTAGATGGAAGCATTTACTTGGACATATTGAATGAAAATTGGTCTTCAGTATTGACTATTGAGAAAGTTCTAATTTCAATATGCTCAGTCCTTTCAAATCCAATTCCTCCAACCTCCATTAATGAAGCTTCAAGAATGTACCTCAATAATTGGCTCAATTTCAATGGGATTGCAAGAGAATGGACCAAAATATATGCAATGCCAAATTGA

Protein sequence

MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTLCPRPNPSPPPSPLPIYDDDRNRPESPIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADISGNEEKTSAMYRIEKEEEVATDCSFGPVAADISGNEKKTSAMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTSINEASRMYLNNWLNFNGIAREWTKIYAMPN
Homology
BLAST of Lsi09G011450 vs. ExPASy Swiss-Prot
Match: P35129 (Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditis elegans OX=6239 GN=let-70 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.4e-36
Identity = 71/146 (48.63%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A+ RI+ EL+ +  +  + CS GPVG D+F W+  ++GP  S Y+GG+F L+I FP+DYP
Sbjct: 2   ALKRIQKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPESPYQGGVFFLTIHFPTDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F T+I+HPN+  +GSI LDIL   WS  LTI KVL+SICS+L +P P    + 
Sbjct: 62  FKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E +R+Y  +   +N +AREWT+ YAM
Sbjct: 122 EIARIYKTDRERYNQLAREWTQKYAM 147

BLAST of Lsi09G011450 vs. ExPASy Swiss-Prot
Match: P25867 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster OX=7227 GN=eff PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.4e-36
Identity = 71/146 (48.63%), Postives = 98/146 (67.12%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A+ RI  EL+ +  +  + CS GPVG D+F W+  ++GP  S Y+GG+F L+I FP+DYP
Sbjct: 2   ALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F T+I+HPN+  +GSI LDIL   WS  LTI KVL+SICS+L +P P    + 
Sbjct: 62  FKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E +R+Y  +   +N +AREWT+ YAM
Sbjct: 122 EIARIYKTDREKYNELAREWTRKYAM 147

BLAST of Lsi09G011450 vs. ExPASy Swiss-Prot
Match: Q2TA10 (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus OX=9913 GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.2e-36
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A+ RI+ EL  +  +  +HCS GPVG D+F W+  ++GP  S Y+GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP + F TKI+HPN+  +GSI LDIL   WS  LT+ KVL+SICS+L +P P    + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           + +++Y ++   +N  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Lsi09G011450 vs. ExPASy Swiss-Prot
Match: P51668 (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens OX=9606 GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.2e-36
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A+ RI+ EL  +  +  +HCS GPVG D+F W+  ++GP  S Y+GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP + F TKI+HPN+  +GSI LDIL   WS  LT+ KVL+SICS+L +P P    + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           + +++Y ++   +N  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Lsi09G011450 vs. ExPASy Swiss-Prot
Match: P61080 (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus OX=10090 GN=Ube2d1 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 3.2e-36
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A+ RI+ EL  +  +  +HCS GPVG D+F W+  ++GP  S Y+GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP + F TKI+HPN+  +GSI LDIL   WS  LT+ KVL+SICS+L +P P    + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           + +++Y ++   +N  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Lsi09G011450 vs. ExPASy TrEMBL
Match: A0A6J1CDR5 (ubiquitin-conjugating enzyme E2 5A-like OS=Momordica charantia OX=3673 GN=LOC111009786 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 2.9e-69
Identity = 153/287 (53.31%), Postives = 179/287 (62.37%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTLCP---RPNPSPPPSPLPIYDDDRNRP 60
           M+LSRLRRCFSSK SSSS+Y RL +PP P    +     RP P PPP P+PI      +P
Sbjct: 1   MVLSRLRRCFSSKSSSSSSYSRLRSPPPPPPLPIYHGHYRPPPIPPPRPVPIAAGAYRQP 60

Query: 61  ESPIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADISGNEEKTSAMYRIEKEEEVA 120
           E+  GDG  G         R+E            P    I G                  
Sbjct: 61  ETTTGDGACG---------RVEEAP---------PRRVSIGGG----------------- 120

Query: 121 TDCSFGPVAADISGNEKKTSAMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPA 180
                       SG+E+KTSAMYRIENEL+ MN+E+A+HCSFGPVGGDIFRWEG+VIGPA
Sbjct: 121 ------------SGDEEKTSAMYRIENELKAMNEESATHCSFGPVGGDIFRWEGLVIGPA 180

Query: 181 CSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEK 240
            SCYE GIF LSI  PSDYP  PP +KF TKIFHPN+  DG+I++DIL ENWS  LTIEK
Sbjct: 181 HSCYEEGIFGLSIDLPSDYPSTPPQIKFQTKIFHPNIEEDGTIHIDILKENWSPALTIEK 240

Query: 241 VLISICSVLSNPIPPTSINEASRMYLNNWLNFNGIAREWTKIYAMPN 285
           +L+SICS+LSNP    SINEAS MY N +  F  +AREWTKIYAMP+
Sbjct: 241 LLLSICSILSNPEAQNSINEASMMYKNEYFRFCEVAREWTKIYAMPS 240

BLAST of Lsi09G011450 vs. ExPASy TrEMBL
Match: A0A6J1KCF2 (ubiquitin-conjugating enzyme E2 E3-like OS=Cucurbita maxima OX=3661 GN=LOC111494344 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 9.5e-68
Identity = 161/305 (52.79%), Postives = 194/305 (63.61%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYT---PPRPIADTLCPRPNPSPPPSPLPIYDDDRNRP 60
           M+L RL  CFS+K SSS+ Y RL+T   PP PI      +P P+    P+P  D+   +P
Sbjct: 1   MVLFRLCCCFSTKSSSSTVYTRLHTPPPPPLPIIHGDYAQPKPA-QQLPIPFVDEGFKQP 60

Query: 61  ES-----PIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPV-AADISGN--EEKTSAMYR 120
           E+     PI  G  G     +    I   +EV       P    +I G+  + K +    
Sbjct: 61  EASPPPLPIIHGDYG-HPNPAQQLPIPIVDEVFKQPEDSPPRVPNIHGDFGQPKPAQKMP 120

Query: 121 IEKEEEVATDCSFGPVAA---------DISGNEKKTSAMYRIENELRVMNDEAASHCSFG 180
           I   +E        P  A         D SGNE+KTS+MYRIENELR MN+ AA+HCSFG
Sbjct: 121 IPTADEGFKQPEASPPRAGTNSEGDGGDGSGNEEKTSSMYRIENELRAMNEVAATHCSFG 180

Query: 181 PVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSI 240
           PVG DIFRWEGVVIGPA SCY+GGIFHLSIQFPS+YPL PP +KFLTKIFHPNV  DG+I
Sbjct: 181 PVGADIFRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTKIFHPNVEEDGTI 240

Query: 241 YLDILNENWSSVLTIEKVLISICSVLSNPIPPT-SINEASRMYLNNWLNFNGIAREWTKI 285
            +DIL +NWS  LTIE +++SICS+LSNP P   SINEASRM+LNNWLN+  IAREWTK 
Sbjct: 241 LIDILMDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNYTKIAREWTKK 300

BLAST of Lsi09G011450 vs. ExPASy TrEMBL
Match: A0A6J1HIF1 (ubiquitin-conjugating enzyme E2 E2-like OS=Cucurbita moschata OX=3662 GN=LOC111464331 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 3.0e-66
Identity = 156/305 (51.15%), Postives = 189/305 (61.97%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTLCPRPNPSPPPS---PLPIYDDDRNRP 60
           M+LSRL  CFS+K SSS+ Y R +TPP P    +       P P+   P+PI D+   +P
Sbjct: 1   MVLSRLCCCFSTKSSSSTVYTRFHTPPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  ES-----PIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADI---SGNEEKTSAMYR 120
           E+     PI  G  G     +    I   +EV+      P    I      +   +    
Sbjct: 61  EASPPRVPIIHGDYG-HLNPAQQLPIPIVDEVSKQPEDSPPRVPIIHGDFGQPMPAQHLP 120

Query: 121 IEKEEEVATDCSFGPVAA---------DISGNEKKTSAMYRIENELRVMNDEAASHCSFG 180
           I   +E        P  A         D SGNE+KTSAMYRIENELR MN+ AA+HCSFG
Sbjct: 121 IPTGDEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAMYRIENELRAMNEAAATHCSFG 180

Query: 181 PVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSI 240
           PVG DI R EG+VIGPA SCY+GGIFHLSIQFPS+YPL PP +KFLTKIFHPN+  DG+I
Sbjct: 181 PVGADILRCEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTKIFHPNIEEDGTI 240

Query: 241 YLDILNENWSSVLTIEKVLISICSVLSNPIPPT-SINEASRMYLNNWLNFNGIAREWTKI 285
            +DIL +NWS  LTIE +++SICS+LSNP P   SINEASRM+LNNWLN+  IAREWTK 
Sbjct: 241 LIDILTDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNYTKIAREWTKQ 300

BLAST of Lsi09G011450 vs. ExPASy TrEMBL
Match: A0A0A0LI75 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138770 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 4.0e-66
Identity = 127/184 (69.02%), Postives = 148/184 (80.43%), Query Frame = 0

Query: 101 EEKTSAMYRIEKEEEVATDCSFGPVAADISGNEKKTSAMYRIENELRVMNDEAASHCSFG 160
           EEKTS    I+ ++E   D S+G      +  EK + AMYRIENEL+ MN+E A+HCSFG
Sbjct: 27  EEKTSWTRGIDHQKE---DISWG------NNEEKSSVAMYRIENELKSMNEEVATHCSFG 86

Query: 161 PVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSI 220
           PVG DIFRWEGVVIGPA SCYEGGIFH+SIQFPSDYP  PP + FLTKIFHPNV +DGSI
Sbjct: 87  PVGDDIFRWEGVVIGPAGSCYEGGIFHVSIQFPSDYPFTPPSINFLTKIFHPNVEVDGSI 146

Query: 221 YLDILNENWSSVLTIEKVLISICSVLSNPIPPTSINEASRMYLNNWLNFNGIAREWTKIY 280
            +DILNENWSS LTIEK+L+SICS+LSNPIP  S NEAS M+LNNWL+FN  A++WTK+Y
Sbjct: 147 GMDILNENWSSALTIEKLLLSICSILSNPIPHGSTNEASTMFLNNWLDFNDTAKQWTKLY 201

Query: 281 AMPN 285
           AMPN
Sbjct: 207 AMPN 201

BLAST of Lsi09G011450 vs. ExPASy TrEMBL
Match: A0A1S3C6C4 (ubiquitin-conjugating enzyme E2 D1-like OS=Cucumis melo OX=3656 GN=LOC103496968 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 5.2e-66
Identity = 122/157 (77.71%), Postives = 135/157 (85.99%), Query Frame = 0

Query: 129 ISGN--EKKTSAMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIF 188
           I GN  EK + AMYRIENEL+ MN E A+HCSFGPVG DIFRWEG+VIGPACSCYEGGIF
Sbjct: 47  IRGNNEEKSSVAMYRIENELKAMNKEVATHCSFGPVGDDIFRWEGIVIGPACSCYEGGIF 106

Query: 189 HLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVL 248
           H+SIQFPSDYP  PP + FLTKIFHPNV LDGSI +DIL ENWSS LTIEK+L+SICSVL
Sbjct: 107 HVSIQFPSDYPFTPPSIHFLTKIFHPNVELDGSIKIDILYENWSSALTIEKLLLSICSVL 166

Query: 249 SNPIPPTSINEASRMYLNNWLNFNGIAREWTKIYAMP 284
           SNPIP  S NEAS M+LN+WLNFN IAR+WTK+YAMP
Sbjct: 167 SNPIPQGSTNEASTMFLNDWLNFNDIARQWTKLYAMP 203

BLAST of Lsi09G011450 vs. NCBI nr
Match: XP_038907075.1 (ubiquitin-conjugating enzyme E2 D1-like [Benincasa hispida])

HSP 1 Score: 322.4 bits (825), Expect = 3.9e-84
Identity = 172/284 (60.56%), Postives = 191/284 (67.25%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTLCPRPNPSPPPSPLPIYDDDRNRPESP 60
           M  SRLRRCFSS PSSSSAYRRLY+PP+PI+DTL P+PNPSP P   PI           
Sbjct: 1   MFPSRLRRCFSSNPSSSSAYRRLYSPPQPISDTLSPQPNPSPSPI-WPI----------S 60

Query: 61  IGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADISGNEEKTSAMYRIEKEEEVATDC 120
           IGDGG                                                       
Sbjct: 61  IGDGG------------------------------------------------------- 120

Query: 121 SFGPVAADISGNEKKTSAMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSC 180
                    SGNE+K+SAMYRIENELR MN+EAA+HCSFGPVG DIFRWEGVVIGPACSC
Sbjct: 121 ---------SGNEEKSSAMYRIENELRAMNEEAATHCSFGPVGDDIFRWEGVVIGPACSC 180

Query: 181 YEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLI 240
           YEGGIF LSIQFPS+YPL PPY+KFLTKIFHPNVG DG+I++DILNENWSS LTIEK+L+
Sbjct: 181 YEGGIFQLSIQFPSNYPLRPPYIKFLTKIFHPNVGSDGTIFMDILNENWSSALTIEKLLL 209

Query: 241 SICSVLSNPIPPTSINEASRMYLNNWLNFNGIAREWTKIYAMPN 285
           SICS+LSNPIP  SINEAS MYL+NWLN++ IAREWTKIYAMPN
Sbjct: 241 SICSILSNPIPQGSINEASTMYLDNWLNYSKIAREWTKIYAMPN 209

BLAST of Lsi09G011450 vs. NCBI nr
Match: XP_022138687.1 (ubiquitin-conjugating enzyme E2 5A-like [Momordica charantia] >XP_022138688.1 ubiquitin-conjugating enzyme E2 5A-like [Momordica charantia])

HSP 1 Score: 271.9 bits (694), Expect = 6.1e-69
Identity = 153/287 (53.31%), Postives = 179/287 (62.37%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTLCP---RPNPSPPPSPLPIYDDDRNRP 60
           M+LSRLRRCFSSK SSSS+Y RL +PP P    +     RP P PPP P+PI      +P
Sbjct: 1   MVLSRLRRCFSSKSSSSSSYSRLRSPPPPPPLPIYHGHYRPPPIPPPRPVPIAAGAYRQP 60

Query: 61  ESPIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADISGNEEKTSAMYRIEKEEEVA 120
           E+  GDG  G         R+E            P    I G                  
Sbjct: 61  ETTTGDGACG---------RVEEAP---------PRRVSIGGG----------------- 120

Query: 121 TDCSFGPVAADISGNEKKTSAMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPA 180
                       SG+E+KTSAMYRIENEL+ MN+E+A+HCSFGPVGGDIFRWEG+VIGPA
Sbjct: 121 ------------SGDEEKTSAMYRIENELKAMNEESATHCSFGPVGGDIFRWEGLVIGPA 180

Query: 181 CSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEK 240
            SCYE GIF LSI  PSDYP  PP +KF TKIFHPN+  DG+I++DIL ENWS  LTIEK
Sbjct: 181 HSCYEEGIFGLSIDLPSDYPSTPPQIKFQTKIFHPNIEEDGTIHIDILKENWSPALTIEK 240

Query: 241 VLISICSVLSNPIPPTSINEASRMYLNNWLNFNGIAREWTKIYAMPN 285
           +L+SICS+LSNP    SINEAS MY N +  F  +AREWTKIYAMP+
Sbjct: 241 LLLSICSILSNPEAQNSINEASMMYKNEYFRFCEVAREWTKIYAMPS 240

BLAST of Lsi09G011450 vs. NCBI nr
Match: XP_023000037.1 (ubiquitin-conjugating enzyme E2 E3-like [Cucurbita maxima])

HSP 1 Score: 266.9 bits (681), Expect = 2.0e-67
Identity = 161/305 (52.79%), Postives = 194/305 (63.61%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYT---PPRPIADTLCPRPNPSPPPSPLPIYDDDRNRP 60
           M+L RL  CFS+K SSS+ Y RL+T   PP PI      +P P+    P+P  D+   +P
Sbjct: 1   MVLFRLCCCFSTKSSSSTVYTRLHTPPPPPLPIIHGDYAQPKPA-QQLPIPFVDEGFKQP 60

Query: 61  ES-----PIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPV-AADISGN--EEKTSAMYR 120
           E+     PI  G  G     +    I   +EV       P    +I G+  + K +    
Sbjct: 61  EASPPPLPIIHGDYG-HPNPAQQLPIPIVDEVFKQPEDSPPRVPNIHGDFGQPKPAQKMP 120

Query: 121 IEKEEEVATDCSFGPVAA---------DISGNEKKTSAMYRIENELRVMNDEAASHCSFG 180
           I   +E        P  A         D SGNE+KTS+MYRIENELR MN+ AA+HCSFG
Sbjct: 121 IPTADEGFKQPEASPPRAGTNSEGDGGDGSGNEEKTSSMYRIENELRAMNEVAATHCSFG 180

Query: 181 PVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSI 240
           PVG DIFRWEGVVIGPA SCY+GGIFHLSIQFPS+YPL PP +KFLTKIFHPNV  DG+I
Sbjct: 181 PVGADIFRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTKIFHPNVEEDGTI 240

Query: 241 YLDILNENWSSVLTIEKVLISICSVLSNPIPPT-SINEASRMYLNNWLNFNGIAREWTKI 285
            +DIL +NWS  LTIE +++SICS+LSNP P   SINEASRM+LNNWLN+  IAREWTK 
Sbjct: 241 LIDILMDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNYTKIAREWTKK 300

BLAST of Lsi09G011450 vs. NCBI nr
Match: KAG6593782.1 (Ubiquitin-conjugating enzyme E2 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 265.4 bits (677), Expect = 5.7e-67
Identity = 157/305 (51.48%), Postives = 192/305 (62.95%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTL---CPRPNPSPPPSPLPIYDDDRNRP 60
           M+LSRL  CFS+K SSS+ Y R +TPP P    +     +P P+    P+PI D+   +P
Sbjct: 1   MVLSRLCCCFSTKFSSSTVYTRFHTPPPPPVPIIHGDYAQPKPA-QQLPIPIVDEGFKQP 60

Query: 61  ES-----PIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADISG---NEEKTSAMYR 120
           E+     PI  G  G     +    I   +EV+      P    I      +   +    
Sbjct: 61  EASPPRVPIIHGDYG-HPNPAQQLPIPIVDEVSKQPEDSPPRVPIMHGDLGQPMPAQHLP 120

Query: 121 IEKEEEVATDCSFGPVAA---------DISGNEKKTSAMYRIENELRVMNDEAASHCSFG 180
           I   +E        P  A         D SGNE+KTSA+YRIENELR MN+ AA+HCSFG
Sbjct: 121 IPTADEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAIYRIENELRAMNEAAATHCSFG 180

Query: 181 PVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSI 240
           PVG DI RWEG+VIGPA SCY+GGIFHLSIQFPS+YPL PP +KFLTKIFHPNV  DG+I
Sbjct: 181 PVGADILRWEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTKIFHPNVEEDGTI 240

Query: 241 YLDILNENWSSVLTIEKVLISICSVLSNPIPPT-SINEASRMYLNNWLNFNGIAREWTKI 285
            +DIL++NWS  LTIE +++SICS+LSNP P   SINEASRM+LNNWLN+  IAREWTK 
Sbjct: 241 LIDILSDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNYTKIAREWTKK 300

BLAST of Lsi09G011450 vs. NCBI nr
Match: XP_022964261.1 (ubiquitin-conjugating enzyme E2 E2-like [Cucurbita moschata])

HSP 1 Score: 261.9 bits (668), Expect = 6.3e-66
Identity = 156/305 (51.15%), Postives = 189/305 (61.97%), Query Frame = 0

Query: 1   MLLSRLRRCFSSKPSSSSAYRRLYTPPRPIADTLCPRPNPSPPPS---PLPIYDDDRNRP 60
           M+LSRL  CFS+K SSS+ Y R +TPP P    +       P P+   P+PI D+   +P
Sbjct: 1   MVLSRLCCCFSTKSSSSTVYTRFHTPPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  ES-----PIGDGGSGIEEKTSAMYRIENKEEVATDCSFGPVAADI---SGNEEKTSAMYR 120
           E+     PI  G  G     +    I   +EV+      P    I      +   +    
Sbjct: 61  EASPPRVPIIHGDYG-HLNPAQQLPIPIVDEVSKQPEDSPPRVPIIHGDFGQPMPAQHLP 120

Query: 121 IEKEEEVATDCSFGPVAA---------DISGNEKKTSAMYRIENELRVMNDEAASHCSFG 180
           I   +E        P  A         D SGNE+KTSAMYRIENELR MN+ AA+HCSFG
Sbjct: 121 IPTGDEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAMYRIENELRAMNEAAATHCSFG 180

Query: 181 PVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYPLYPPYVKFLTKIFHPNVGLDGSI 240
           PVG DI R EG+VIGPA SCY+GGIFHLSIQFPS+YPL PP +KFLTKIFHPN+  DG+I
Sbjct: 181 PVGADILRCEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTKIFHPNIEEDGTI 240

Query: 241 YLDILNENWSSVLTIEKVLISICSVLSNPIPPT-SINEASRMYLNNWLNFNGIAREWTKI 285
            +DIL +NWS  LTIE +++SICS+LSNP P   SINEASRM+LNNWLN+  IAREWTK 
Sbjct: 241 LIDILTDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWLNYTKIAREWTKQ 300

BLAST of Lsi09G011450 vs. TAIR 10
Match: AT5G41700.2 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 146.7 bits (369), Expect = 2.7e-35
Identity = 70/146 (47.95%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A  RI  EL+ +  +  + CS GPV  D+F W+  ++GPA S Y GG+F ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F TK+FHPN+  +GSI LDIL E WS  LTI KVL+SICS+L++P P    + 
Sbjct: 62  FKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E + MY  +   +   AR WT+ YAM
Sbjct: 122 EIAHMYKTDRAKYEATARNWTQKYAM 147

BLAST of Lsi09G011450 vs. TAIR 10
Match: AT5G41700.1 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 146.7 bits (369), Expect = 2.7e-35
Identity = 70/146 (47.95%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A  RI  EL+ +  +  + CS GPV  D+F W+  ++GPA S Y GG+F ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F TK+FHPN+  +GSI LDIL E WS  LTI KVL+SICS+L++P P    + 
Sbjct: 62  FKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E + MY  +   +   AR WT+ YAM
Sbjct: 122 EIAHMYKTDRAKYEATARNWTQKYAM 147

BLAST of Lsi09G011450 vs. TAIR 10
Match: AT5G41700.5 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 146.7 bits (369), Expect = 2.7e-35
Identity = 70/146 (47.95%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A  RI  EL+ +  +  + CS GPV  D+F W+  ++GPA S Y GG+F ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F TK+FHPN+  +GSI LDIL E WS  LTI KVL+SICS+L++P P    + 
Sbjct: 62  FKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E + MY  +   +   AR WT+ YAM
Sbjct: 122 EIAHMYKTDRAKYEATARNWTQKYAM 147

BLAST of Lsi09G011450 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 146.4 bits (368), Expect = 3.6e-35
Identity = 70/146 (47.95%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A  RI  EL+ +  +  + CS GPV  D+F W+  ++GP+ S Y GG+F ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F TK+FHPNV  +GSI LDIL E WS  LTI KVL+SICS+L++P P    + 
Sbjct: 62  FKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E + MY  +   +   AR WT+ YAM
Sbjct: 122 EIAHMYKTDRAKYESTARSWTQKYAM 147

BLAST of Lsi09G011450 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 146.4 bits (368), Expect = 3.6e-35
Identity = 70/146 (47.95%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 138 AMYRIENELRVMNDEAASHCSFGPVGGDIFRWEGVVIGPACSCYEGGIFHLSIQFPSDYP 197
           A  RI  EL+ +  +  + CS GPV  D+F W+  ++GP+ S Y GG+F ++I FP DYP
Sbjct: 2   ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 61

Query: 198 LYPPYVKFLTKIFHPNVGLDGSIYLDILNENWSSVLTIEKVLISICSVLSNPIPPTS-IN 257
             PP V F TK+FHPNV  +GSI LDIL E WS  LTI KVL+SICS+L++P P    + 
Sbjct: 62  FKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVP 121

Query: 258 EASRMYLNNWLNFNGIAREWTKIYAM 283
           E + MY  +   +   AR WT+ YAM
Sbjct: 122 EIAHMYKTDRAKYESTARSWTQKYAM 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P351292.4e-3648.63Ubiquitin-conjugating enzyme E2 2 OS=Caenorhabditis elegans OX=6239 GN=let-70 PE... [more]
P258672.4e-3648.63Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster OX=7227 GN=eff... [more]
Q2TA103.2e-3646.58Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus OX=9913 GN=UBE2D1 PE=2 SV=1[more]
P516683.2e-3646.58Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens OX=9606 GN=UBE2D1 PE=1 SV=1[more]
P610803.2e-3646.58Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus OX=10090 GN=Ube2d1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CDR52.9e-6953.31ubiquitin-conjugating enzyme E2 5A-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1KCF29.5e-6852.79ubiquitin-conjugating enzyme E2 E3-like OS=Cucurbita maxima OX=3661 GN=LOC111494... [more]
A0A6J1HIF13.0e-6651.15ubiquitin-conjugating enzyme E2 E2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A0A0LI754.0e-6669.02UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138770 PE... [more]
A0A1S3C6C45.2e-6677.71ubiquitin-conjugating enzyme E2 D1-like OS=Cucumis melo OX=3656 GN=LOC103496968 ... [more]
Match NameE-valueIdentityDescription
XP_038907075.13.9e-8460.56ubiquitin-conjugating enzyme E2 D1-like [Benincasa hispida][more]
XP_022138687.16.1e-6953.31ubiquitin-conjugating enzyme E2 5A-like [Momordica charantia] >XP_022138688.1 ub... [more]
XP_023000037.12.0e-6752.79ubiquitin-conjugating enzyme E2 E3-like [Cucurbita maxima][more]
KAG6593782.15.7e-6751.48Ubiquitin-conjugating enzyme E2 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022964261.16.3e-6651.15ubiquitin-conjugating enzyme E2 E2-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G41700.22.7e-3547.95ubiquitin conjugating enzyme 8 [more]
AT5G41700.12.7e-3547.95ubiquitin conjugating enzyme 8 [more]
AT5G41700.52.7e-3547.95ubiquitin conjugating enzyme 8 [more]
AT1G64230.13.6e-3547.95ubiquitin-conjugating enzyme 28 [more]
AT1G64230.23.6e-3547.95ubiquitin-conjugating enzyme 28 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 140..282
e-value: 1.3E-44
score: 164.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..50
NoneNo IPR availablePANTHERPTHR24068:SF242UBIQUITIN CARRIER PROTEINcoord: 140..282
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 140..282
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 141..274
e-value: 3.7E-36
score: 123.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 137..282
score: 41.23307
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 141..277
e-value: 1.2106E-46
score: 151.201
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 128..284
e-value: 9.0E-49
score: 167.1
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 130..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G011450.1Lsi09G011450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity