Lsi09G010700 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G010700
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr09: 16630100 .. 16631527 (+)
RNA-Seq ExpressionLsi09G010700
SyntenyLsi09G010700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTCTTTCGGATCTCTTACCCCCGATCTTCATCATTTCGTCTCAAGATCTCTACCTTATCTACCTTGCAGCTAAGTACAGTCTCTTCTGCTGATTTATTCTATGATCATCTGCAGAAAAACAATGGTAATGTGGAGAAAACCCTTGCTACGGTAAAGACCAAGTTGGATTCTAGATGTGTCAACCAAGTATTACATAAATGTTCCTTCGAACTATCTCAAATGGGTCTTCGATTTTTTATATGGGCTGGTCGACAGCCTAATTATAGGCATAGCTCTTTTATATATAGTAGAGCTTGTGAACTGATTGGAATTAATGGAAGCCCATGTTTGCTTTCTAATGTTATTGAAGATTATAGAAGAGAGGGTTGCCTTGTTGATATTAGGATGTTTAAGATTATTTTAAACTTGTGTAAAGAAGCTAAGCTTGCCAAAGAGGCTTTGTCCATTTTAGGGAAAATGCCTGAATTTCATTTGCATGCTGACACTACAATGTATAATCTGGTTATAAGGTTATTTACTGAGAACGGTGAGATGGATAAGGCGATGGAGTTGATGAAAGAGATGGATTCAGTTGATATACATCCTAACATGATCACTTATATTTCTTTGCTCAAAGGATTCTGTGATGTAGGTCGTTGGGAGGATGCTTATGGGTTATTTAAGGCTATGAAGGAAAATGGATGTGCACCCAATACAGTGGTTTACTCTGTGCTACTTAATGCCGCCAGTCGGCATGGGATTATGGAAAAGCTAATGGAAATGTTGGAGGAGATGGAAAAACAAGGAGGAACTTGCGGTCCAAATACTGTCACATACACTTCCATAATCCAGAGTCTATGTGAACTAGGCCAGCCTCTGGAGGCATTGAAGATATTGGACAGAATGGAAGACTCTGGTTGTGCTCCAAATCGTGTTACAGTTAGCTCTTTAATCAAGGAATTTTGTAAAAATGGTCACGTTGAGGAGGCTTATAAGTTGATTGATAGAGTTGTTGCAAGAGGTGGTGCTTCATATGGTGATTGCTATAGCTCACTTGTGGTATCTTTGGTTAGGATGAAAAAGATTGCAGAGGCAGAGGAGCTATTTAGAAACATGTTAGCCAATGGGGTGAAGCCAGATGGTGTGGCTTGCACTCTCATGATCAAGGAATTGTGCTTAGAGGAGCGGGTGCTAGATGGTTTTAACTTATGCAACGAAGTCGATAGGAATGGATATTTATCTTCCATTGACTCTGATATTTATTCTCTTCTTTTAGTTGGACTTTTTGAGCATGACCACTCTGTGGATGCTGAAAAACTTGCAAGGTTGATGCTTAAAAAAGGGATTCGTTTAAAACCTCACTATGCTGAAAGTATCATGAAACATCTAAAGAAATTTGAAGACCAAGACTTAGTTATGCATTTGGGTGGAATAAGGAAATGA

mRNA sequence

ATGGCTCTCTTTCGGATCTCTTACCCCCGATCTTCATCATTTCGTCTCAAGATCTCTACCTTATCTACCTTGCAGCTAAGTACAGTCTCTTCTGCTGATTTATTCTATGATCATCTGCAGAAAAACAATGGTAATGTGGAGAAAACCCTTGCTACGGTAAAGACCAAGTTGGATTCTAGATGTGTCAACCAAGTATTACATAAATGTTCCTTCGAACTATCTCAAATGGGTCTTCGATTTTTTATATGGGCTGGTCGACAGCCTAATTATAGGCATAGCTCTTTTATATATAGTAGAGCTTGTGAACTGATTGGAATTAATGGAAGCCCATGTTTGCTTTCTAATGTTATTGAAGATTATAGAAGAGAGGGTTGCCTTGTTGATATTAGGATGTTTAAGATTATTTTAAACTTGTGTAAAGAAGCTAAGCTTGCCAAAGAGGCTTTGTCCATTTTAGGGAAAATGCCTGAATTTCATTTGCATGCTGACACTACAATGTATAATCTGGTTATAAGGTTATTTACTGAGAACGGTGAGATGGATAAGGCGATGGAGTTGATGAAAGAGATGGATTCAGTTGATATACATCCTAACATGATCACTTATATTTCTTTGCTCAAAGGATTCTGTGATGTAGGTCGTTGGGAGGATGCTTATGGGTTATTTAAGGCTATGAAGGAAAATGGATGTGCACCCAATACAGTGGTTTACTCTGTGCTACTTAATGCCGCCAGTCGGCATGGGATTATGGAAAAGCTAATGGAAATGTTGGAGGAGATGGAAAAACAAGGAGGAACTTGCGGTCCAAATACTGTCACATACACTTCCATAATCCAGAGTCTATGTGAACTAGGCCAGCCTCTGGAGGCATTGAAGATATTGGACAGAATGGAAGACTCTGGTTGTGCTCCAAATCGTGTTACAGTTAGCTCTTTAATCAAGGAATTTTGTAAAAATGGTCACGTTGAGGAGGCTTATAAGTTGATTGATAGAGTTGTTGCAAGAGGTGGTGCTTCATATGGTGATTGCTATAGCTCACTTGTGGTATCTTTGGTTAGGATGAAAAAGATTGCAGAGGCAGAGGAGCTATTTAGAAACATGTTAGCCAATGGGGTGAAGCCAGATGGTGTGGCTTGCACTCTCATGATCAAGGAATTGTGCTTAGAGGAGCGGGTGCTAGATGGTTTTAACTTATGCAACGAAGTCGATAGGAATGGATATTTATCTTCCATTGACTCTGATATTTATTCTCTTCTTTTAGTTGGACTTTTTGAGCATGACCACTCTGTGGATGCTGAAAAACTTGCAAGGTTGATGCTTAAAAAAGGGATTCGTTTAAAACCTCACTATGCTGAAAGTATCATGAAACATCTAAAGAAATTTGAAGACCAAGACTTAGTTATGCATTTGGGTGGAATAAGGAAATGA

Coding sequence (CDS)

ATGGCTCTCTTTCGGATCTCTTACCCCCGATCTTCATCATTTCGTCTCAAGATCTCTACCTTATCTACCTTGCAGCTAAGTACAGTCTCTTCTGCTGATTTATTCTATGATCATCTGCAGAAAAACAATGGTAATGTGGAGAAAACCCTTGCTACGGTAAAGACCAAGTTGGATTCTAGATGTGTCAACCAAGTATTACATAAATGTTCCTTCGAACTATCTCAAATGGGTCTTCGATTTTTTATATGGGCTGGTCGACAGCCTAATTATAGGCATAGCTCTTTTATATATAGTAGAGCTTGTGAACTGATTGGAATTAATGGAAGCCCATGTTTGCTTTCTAATGTTATTGAAGATTATAGAAGAGAGGGTTGCCTTGTTGATATTAGGATGTTTAAGATTATTTTAAACTTGTGTAAAGAAGCTAAGCTTGCCAAAGAGGCTTTGTCCATTTTAGGGAAAATGCCTGAATTTCATTTGCATGCTGACACTACAATGTATAATCTGGTTATAAGGTTATTTACTGAGAACGGTGAGATGGATAAGGCGATGGAGTTGATGAAAGAGATGGATTCAGTTGATATACATCCTAACATGATCACTTATATTTCTTTGCTCAAAGGATTCTGTGATGTAGGTCGTTGGGAGGATGCTTATGGGTTATTTAAGGCTATGAAGGAAAATGGATGTGCACCCAATACAGTGGTTTACTCTGTGCTACTTAATGCCGCCAGTCGGCATGGGATTATGGAAAAGCTAATGGAAATGTTGGAGGAGATGGAAAAACAAGGAGGAACTTGCGGTCCAAATACTGTCACATACACTTCCATAATCCAGAGTCTATGTGAACTAGGCCAGCCTCTGGAGGCATTGAAGATATTGGACAGAATGGAAGACTCTGGTTGTGCTCCAAATCGTGTTACAGTTAGCTCTTTAATCAAGGAATTTTGTAAAAATGGTCACGTTGAGGAGGCTTATAAGTTGATTGATAGAGTTGTTGCAAGAGGTGGTGCTTCATATGGTGATTGCTATAGCTCACTTGTGGTATCTTTGGTTAGGATGAAAAAGATTGCAGAGGCAGAGGAGCTATTTAGAAACATGTTAGCCAATGGGGTGAAGCCAGATGGTGTGGCTTGCACTCTCATGATCAAGGAATTGTGCTTAGAGGAGCGGGTGCTAGATGGTTTTAACTTATGCAACGAAGTCGATAGGAATGGATATTTATCTTCCATTGACTCTGATATTTATTCTCTTCTTTTAGTTGGACTTTTTGAGCATGACCACTCTGTGGATGCTGAAAAACTTGCAAGGTTGATGCTTAAAAAAGGGATTCGTTTAAAACCTCACTATGCTGAAAGTATCATGAAACATCTAAAGAAATTTGAAGACCAAGACTTAGTTATGCATTTGGGTGGAATAAGGAAATGA

Protein sequence

MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSRCVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDYRREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK
Homology
BLAST of Lsi09G010700 vs. ExPASy Swiss-Prot
Match: Q9LVS3 (Pentatricopeptide repeat-containing protein At5g47360 OS=Arabidopsis thaliana OX=3702 GN=At5g47360 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 8.0e-117
Identity = 217/461 (47.07%), Postives = 314/461 (68.11%), Query Frame = 0

Query: 11  SSSFRLKISTLSTLQ-LSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSRCVNQVLHKC 70
           S S R + S +S L+ L+TVS+A+  Y  LQ    N+EK LA+   +LDS C+N+VL +C
Sbjct: 11  SPSLRSQPSKISALRFLTTVSAAERLYGQLQGCTSNLEKELASANVQLDSSCINEVLRRC 70

Query: 71  SFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDYRREGCLVDI 130
                Q GLRFFIWAG   ++RHS+++Y++AC+++ I   P L+  VIE YR+E C V++
Sbjct: 71  DPNQFQSGLRFFIWAGTLSSHRHSAYMYTKACDILKIRAKPDLIKYVIESYRKEECFVNV 130

Query: 131 RMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKE 190
           +  +I+L LC +A LA EAL +L K PEF++ ADT  YNLVIRLF + G+++ A  L+KE
Sbjct: 131 KTMRIVLTLCNQANLADEALWVLRKFPEFNVCADTVAYNLVIRLFADKGDLNIADMLIKE 190

Query: 191 MDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGI 250
           MD V ++P++ITY S++ G+C+ G+ +DA+ L K M ++ C  N+V YS +L    + G 
Sbjct: 191 MDCVGLYPDVITYTSMINGYCNAGKIDDAWRLAKEMSKHDCVLNSVTYSRILEGVCKSGD 250

Query: 251 MEKLMEMLEEMEKQ--GGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRV 310
           ME+ +E+L EMEK+  GG   PN VTYT +IQ+ CE  +  EAL +LDRM + GC PNRV
Sbjct: 251 MERALELLAEMEKEDGGGLISPNAVTYTLVIQAFCEKRRVEEALLVLDRMGNRGCMPNRV 310

Query: 311 TVSSLIKEFCKNGH-VEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRN 370
           T   LI+   +N   V+   KLID++V  GG S  +C+SS  VSL+RMK+  EAE++FR 
Sbjct: 311 TACVLIQGVLENDEDVKALSKLIDKLVKLGGVSLSECFSSATVSLIRMKRWEEAEKIFRL 370

Query: 371 MLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEH 430
           ML  GV+PDG+AC+ + +ELCL ER LD F L  E+++    S+IDSDI+++LL+GL + 
Sbjct: 371 MLVRGVRPDGLACSHVFRELCLLERYLDCFLLYQEIEKKDVKSTIDSDIHAVLLLGLCQQ 430

Query: 431 DHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLV 468
            +S +A KLA+ ML K +RLK  + E I++ LKK  D+DL+
Sbjct: 431 GNSWEAAKLAKSMLDKKMRLKVSHVEKIIEALKKTGDEDLM 471

BLAST of Lsi09G010700 vs. ExPASy Swiss-Prot
Match: Q9LPX2 (Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g12775 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.9e-38
Identity = 90/313 (28.75%), Postives = 172/313 (54.95%), Query Frame = 0

Query: 135 ILN-LCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKEMDSV 194
           +LN +CK  + A  A+ +L KM E ++  D   Y+++I    ++G +D A  L  EM+  
Sbjct: 234 VLNVMCKSGQTAL-AMELLRKMEERNIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIK 293

Query: 195 DIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGIMEKL 254
               ++ITY +L+ GFC+ GRW+D   L + M +   +PN V +SVL+++  + G + + 
Sbjct: 294 GFKADIITYNTLIGGFCNAGRWDDGAKLLRDMIKRKISPNVVTFSVLIDSFVKEGKLREA 353

Query: 255 MEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRVTVSSLI 314
            ++L+EM ++G    PNT+TY S+I   C+  +  EA++++D M   GC P+ +T + LI
Sbjct: 354 DQLLKEMMQRG--IAPNTITYNSLIDGFCKENRLEEAIQMVDLMISKGCDPDIMTFNILI 413

Query: 315 KEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRNMLANGVK 374
             +CK   +++  +L   +  RG  +    Y++LV    +  K+  A++LF+ M++  V+
Sbjct: 414 NGYCKANRIDDGLELFREMSLRGVIANTVTYNTLVQGFCQSGKLEVAKKLFQEMVSRRVR 473

Query: 375 PDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEHDHSVDAE 434
           PD V+  +++  LC    +     +  +++++     +D  IY +++ G+       DA 
Sbjct: 474 PDIVSYKILLDGLCDNGELEKALEIFGKIEKS--KMELDIGIYMIIIHGMCNASKVDDAW 533

Query: 435 KLARLMLKKGIRL 447
            L   +  KG++L
Sbjct: 534 DLFCSLPLKGVKL 541

BLAST of Lsi09G010700 vs. ExPASy Swiss-Prot
Match: Q0WKV3 (Pentatricopeptide repeat-containing protein At1g12300, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g12300 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.3e-37
Identity = 100/362 (27.62%), Postives = 185/362 (51.10%), Query Frame = 0

Query: 85  GRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDYRREGCLVDIRMFKIILN-LCKEAK 144
           G +P+    + + +  C L G      LL + + +Y   GC  +   +  +LN +CK  +
Sbjct: 188 GHKPDLITINTLVNGLC-LSGKEAEAMLLIDKMVEY---GCQPNAVTYGPVLNVMCKSGQ 247

Query: 145 LAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKEMDSVDIHPNMITYI 204
            A  A+ +L KM E ++  D   Y+++I    ++G +D A  L  EM+   I  N+ITY 
Sbjct: 248 TAL-AMELLRKMEERNIKLDAVKYSIIIDGLCKHGSLDNAFNLFNEMEMKGITTNIITYN 307

Query: 205 SLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGIMEKLMEMLEEMEKQ 264
            L+ GFC+ GRW+D   L + M +    PN V +SVL+++  + G + +  E+ +EM  +
Sbjct: 308 ILIGGFCNAGRWDDGAKLLRDMIKRKINPNVVTFSVLIDSFVKEGKLREAEELHKEMIHR 367

Query: 265 GGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRVTVSSLIKEFCKNGHVE 324
           G    P+T+TYTS+I   C+     +A +++D M   GC PN  T + LI  +CK   ++
Sbjct: 368 G--IAPDTITYTSLIDGFCKENHLDKANQMVDLMVSKGCDPNIRTFNILINGYCKANRID 427

Query: 325 EAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRNMLANGVKPDGVACTLMI 384
           +  +L  ++  RG  +    Y++L+     + K+  A+ELF+ M++  V P+ V   +++
Sbjct: 428 DGLELFRKMSLRGVVADTVTYNTLIQGFCELGKLNVAKELFQEMVSRKVPPNIVTYKILL 487

Query: 385 KELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEHDHSVDAEKLARLMLKKG 444
             LC          +  +++++     +D  IY++++ G+       DA  L   +  KG
Sbjct: 488 DGLCDNGESEKALEIFEKIEKS--KMELDIGIYNIIIHGMCNASKVDDAWDLFCSLPLKG 540

Query: 445 IR 446
           ++
Sbjct: 548 VK 540

BLAST of Lsi09G010700 vs. ExPASy Swiss-Prot
Match: O04491 (Putative pentatricopeptide repeat-containing protein At1g09680 OS=Arabidopsis thaliana OX=3702 GN=At1g09680 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 4.8e-37
Identity = 89/322 (27.64%), Postives = 169/322 (52.48%), Query Frame = 0

Query: 124 GCLVDIRMFKIILN-LCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDK 183
           G  +++ +F I++N  CKE  ++ +A  +  ++ +  L      +N +I  + + G +D+
Sbjct: 235 GFPLNVYVFNILMNKFCKEGNIS-DAQKVFDEITKRSLQPTVVSFNTLINGYCKVGNLDE 294

Query: 184 AMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLN 243
              L  +M+     P++ TY +L+   C   + + A+GLF  M + G  PN V+++ L++
Sbjct: 295 GFRLKHQMEKSRTRPDVFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFTTLIH 354

Query: 244 AASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGC 303
             SR+G ++ + E  ++M  +G    P+ V Y +++   C+ G  + A  I+D M   G 
Sbjct: 355 GHSRNGEIDLMKESYQKMLSKG--LQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRRGL 414

Query: 304 APNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEE 363
            P+++T ++LI  FC+ G VE A ++   +   G       +S+LV  + +  ++ +AE 
Sbjct: 415 RPDKITYTTLIDGFCRGGDVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDAER 474

Query: 364 LFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVG 423
             R ML  G+KPD V  T+M+   C +     GF L  E+  +G++ S+ +  Y++LL G
Sbjct: 475 ALREMLRAGIKPDDVTYTMMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVT--YNVLLNG 534

Query: 424 LFEHDHSVDAEKLARLMLKKGI 445
           L +     +A+ L   ML  G+
Sbjct: 535 LCKLGQMKNADMLLDAMLNIGV 551

BLAST of Lsi09G010700 vs. ExPASy Swiss-Prot
Match: P0C7Q7 (Putative pentatricopeptide repeat-containing protein At1g12700, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g12700 PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 6.3e-37
Identity = 97/325 (29.85%), Postives = 170/325 (52.31%), Query Frame = 0

Query: 124 GCLVDIRMFKIILN-LCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDK 183
           GC  D+  +  I+N +C+    +  AL +L KM E ++ AD   Y+ +I     +G +D 
Sbjct: 188 GCQPDVVTYNSIVNGICRSGDTSL-ALDLLRKMEERNVKADVFTYSTIIDSLCRDGCIDA 247

Query: 184 AMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLN 243
           A+ L KEM++  I  +++TY SL++G C  G+W D   L K M      PN + ++VLL+
Sbjct: 248 AISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSREIVPNVITFNVLLD 307

Query: 244 AASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGC 303
              + G +++  E+ +EM  +G    PN +TY +++   C   +  EA  +LD M  + C
Sbjct: 308 VFVKEGKLQEANELYKEMITRG--ISPNIITYNTLMDGYCMQNRLSEANNMLDLMVRNKC 367

Query: 304 APNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEE 363
           +P+ VT +SLIK +C    V++  K+   +  RG  +    YS LV    +  KI  AEE
Sbjct: 368 SPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFCQSGKIKLAEE 427

Query: 364 LFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDI--YSLLL 423
           LF+ M+++GV PD +   +++  LC   ++     +  ++ +    S +D  I  Y+ ++
Sbjct: 428 LFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKALEIFEDLQK----SKMDLGIVMYTTII 487

Query: 424 VGLFEHDHSVDAEKLARLMLKKGIR 446
            G+ +     DA  L   +  KG++
Sbjct: 488 EGMCKGGKVEDAWNLFCSLPCKGVK 505

BLAST of Lsi09G010700 vs. ExPASy TrEMBL
Match: A0A6J1EQI2 (pentatricopeptide repeat-containing protein At5g47360 OS=Cucurbita moschata OX=3662 GN=LOC111435690 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 9.1e-249
Identity = 427/475 (89.89%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQK NGNVEKTLATVKTKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKKNGNVEKTLATVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVNQVLHKCS ELSQMGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCLL NVIEDY
Sbjct: 61  CVNQVLHKCSLELSQMGLRFFIWAGRQPNYRHSSFMYTRACELIGLNRSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDI MFK+ILNLCKE KLAKEALSILGKM EFHL ADTTMYNLVIRLFTE GEM
Sbjct: 121 RREGCLVDIGMFKVILNLCKEGKLAKEALSILGKMAEFHLRADTTMYNLVIRLFTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MK+NGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKDNGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGGTCGPNTVTYTSIIQSLCE+GQPLEALKILDRMED 
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGTCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDF 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GCAPNRVTVS L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCAPNRVTVSVLVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLEERV+DGFNLCNEVDRNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVVDGFNLCNEVDRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF DQ+LVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGDQNLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. ExPASy TrEMBL
Match: A0A6J1I125 (pentatricopeptide repeat-containing protein At5g47360 OS=Cucurbita maxima OX=3661 GN=LOC111468919 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.0e-248
Identity = 425/475 (89.47%), Postives = 452/475 (95.16%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATV+TKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVRTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVNQVLHKCSFELS MGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCLL NVIEDY
Sbjct: 61  CVNQVLHKCSFELSPMGLRFFIWAGRQPNYRHSSFMYARACELIGLNRSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCL+DI MFK+ILNLCKE KLAKEALSILGKM EFHL ADTTMYNLVIRLFTE GEM
Sbjct: 121 RREGCLLDIGMFKVILNLCKEGKLAKEALSILGKMAEFHLRADTTMYNLVIRLFTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MKENGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKENGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGG CGPNTVTYTSIIQSLCE+GQPLEALKILDRMEDS
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGNCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDS 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GC+PNRVTVS+L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCSPNRVTVSALVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLEERV+DGFNLCNEV+RNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVVDGFNLCNEVNRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF  QDLVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGGQDLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. ExPASy TrEMBL
Match: A0A0A0LI44 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G123590 PE=4 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 418/475 (88.00%), Postives = 447/475 (94.11%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRIS PRSSSF L ISTLST  L+T+SS+DLFYDHL+K+NGN++KTLAT+KTKLDSR
Sbjct: 1   MALFRISCPRSSSFLLNISTLSTFHLNTLSSSDLFYDHLEKSNGNLDKTLATLKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVN+VL+KCSFELSQMGLRFFIWAGRQPNYRHSSF+YSRACELIGIN SPCLL NVIEDY
Sbjct: 61  CVNEVLYKCSFELSQMGLRFFIWAGRQPNYRHSSFMYSRACELIGINVSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDIRMFKIILNLCKEAKLAKEALSIL KM EFHL ADTTMYNLVIRLFTE GEM
Sbjct: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILRKMSEFHLRADTTMYNLVIRLFTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMELMKEMDSVDIHPNMITYIS+LKGFCDVGRWEDAYGLFK MKENGCAPNTVVYSVL
Sbjct: 181 DKAMELMKEMDSVDIHPNMITYISMLKGFCDVGRWEDAYGLFKDMKENGCAPNTVVYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           +N A R  IM++LMEML+EMEKQGGTC PNTVTYTSIIQSLCE G PLEALK+LDRME+ 
Sbjct: 241 VNGAIRLRIMDRLMEMLKEMEKQGGTCSPNTVTYTSIIQSLCEEGHPLEALKVLDRMEEY 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           G APNRV VS L+KEFCK+GHVEEAYKLIDRVVARGG SYGDCYSSLVV+LV+MKKIAEA
Sbjct: 301 GYAPNRVAVSFLVKEFCKDGHVEEAYKLIDRVVARGGVSYGDCYSSLVVTLVKMKKIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           E+LFRNMLANGVKPDGVAC+LMI+ELCLEERVLDGFNLC EVDRNGYL SID+DIYSLLL
Sbjct: 361 EKLFRNMLANGVKPDGVACSLMIRELCLEERVLDGFNLCYEVDRNGYLCSIDADIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLMLKKGIRLKPHYAESI+KHLKKFED++LVMHLGGIRK
Sbjct: 421 VGLCEHDHSVDAAKLARLMLKKGIRLKPHYAESIIKHLKKFEDRELVMHLGGIRK 475

BLAST of Lsi09G010700 vs. ExPASy TrEMBL
Match: A0A1S3B4L9 (pentatricopeptide repeat-containing protein At5g47360 OS=Cucumis melo OX=3656 GN=LOC103485755 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.7e-235
Identity = 409/475 (86.11%), Postives = 440/475 (92.63%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRISYPRSSS  L ISTLST  LST+SS+DLFYDHL+KNNGNVEKTLATVKTKLDSR
Sbjct: 1   MALFRISYPRSSSILLNISTLSTFHLSTLSSSDLFYDHLEKNNGNVEKTLATVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVN+VL+KCS ELSQMGLRFFIWAGRQPNYRH+SF+YSRACELIGIN SPCLL NVIEDY
Sbjct: 61  CVNEVLYKCSSELSQMGLRFFIWAGRQPNYRHTSFMYSRACELIGINVSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDIR+F+IILNLCKEAKL KEALSIL KM EFHL ADTT+YNLVIRL TE GEM
Sbjct: 121 RREGCLVDIRIFQIILNLCKEAKLTKEALSILRKMSEFHLRADTTIYNLVIRLCTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMELMKEMDSVDIHPNMITYIS++KGFCDVGRWEDAYGLFKAMKENG APNTVVYSVL
Sbjct: 181 DKAMELMKEMDSVDIHPNMITYISMIKGFCDVGRWEDAYGLFKAMKENGYAPNTVVYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           +N A R  IM+KLMEMLEEMEKQGGTC PNTVTYTSIIQSLCE G  LEALK+LDRME+ 
Sbjct: 241 VNGAVRLRIMDKLMEMLEEMEKQGGTCRPNTVTYTSIIQSLCEQGFLLEALKVLDRMEEY 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           G APNRV V  L+KEFCK+GHVEEAYKLIDRVVARGGASYGDC SSLV+SLV+MKKI EA
Sbjct: 301 GHAPNRVAVGYLVKEFCKDGHVEEAYKLIDRVVARGGASYGDCCSSLVISLVKMKKIPEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           E+LFRNMLANGVKPDGVAC+LMI+ELCLEERVLDGF+LC EVDRNGYL  ID+D+YSLLL
Sbjct: 361 EKLFRNMLANGVKPDGVACSLMIRELCLEERVLDGFSLCYEVDRNGYLCYIDADVYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL++HDHSVDA  LARLMLKKGIRLKPHYAESI+KHLKKFEDQ+L+MHLGGIRK
Sbjct: 421 VGLYQHDHSVDAAILARLMLKKGIRLKPHYAESIIKHLKKFEDQELIMHLGGIRK 475

BLAST of Lsi09G010700 vs. ExPASy TrEMBL
Match: A0A5A7SSI7 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold340G00410 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.7e-235
Identity = 409/475 (86.11%), Postives = 440/475 (92.63%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRISYPRSSS  L ISTLST  LST+SS+DLFYDHL+KNNGNVEKTLATVKTKLDSR
Sbjct: 1   MALFRISYPRSSSILLNISTLSTFHLSTLSSSDLFYDHLEKNNGNVEKTLATVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVN+VL+KCS ELSQMGLRFFIWAGRQPNYRH+SF+YSRACELIGIN SPCLL NVIEDY
Sbjct: 61  CVNEVLYKCSSELSQMGLRFFIWAGRQPNYRHTSFMYSRACELIGINVSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDIR+F+IILNLCKEAKL KEALSIL KM EFHL ADTT+YNLVIRL TE GEM
Sbjct: 121 RREGCLVDIRIFQIILNLCKEAKLTKEALSILRKMSEFHLRADTTIYNLVIRLCTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMELMKEMDSVDIHPNMITYIS++KGFCDVGRWEDAYGLFKAMKENG APNTVVYSVL
Sbjct: 181 DKAMELMKEMDSVDIHPNMITYISMIKGFCDVGRWEDAYGLFKAMKENGYAPNTVVYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           +N A R  IM+KLMEMLEEMEKQGGTC PNTVTYTSIIQSLCE G  LEALK+LDRME+ 
Sbjct: 241 VNGAVRLRIMDKLMEMLEEMEKQGGTCRPNTVTYTSIIQSLCEQGFLLEALKVLDRMEEY 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           G APNRV V  L+KEFCK+GHVEEAYKLIDRVVARGGASYGDC SSLV+SLV+MKKI EA
Sbjct: 301 GHAPNRVAVGYLVKEFCKDGHVEEAYKLIDRVVARGGASYGDCCSSLVISLVKMKKIPEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           E+LFRNMLANGVKPDGVAC+LMI+ELCLEERVLDGF+LC EVDRNGYL  ID+D+YSLLL
Sbjct: 361 EKLFRNMLANGVKPDGVACSLMIRELCLEERVLDGFSLCYEVDRNGYLCYIDADVYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL++HDHSVDA  LARLMLKKGIRLKPHYAESI+KHLKKFEDQ+L+MHLGGIRK
Sbjct: 421 VGLYQHDHSVDAAILARLMLKKGIRLKPHYAESIIKHLKKFEDQELIMHLGGIRK 475

BLAST of Lsi09G010700 vs. NCBI nr
Match: KAG7017159.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 872.5 bits (2253), Expect = 1.7e-249
Identity = 428/475 (90.11%), Postives = 453/475 (95.37%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCLL NVIEDY
Sbjct: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFMYTRACELIGLNRSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDI MFK+ILNLCKE KLAKEALSILGKM EFHL ADTTMYNLVIRLFTE G+M
Sbjct: 121 RREGCLVDIGMFKVILNLCKEGKLAKEALSILGKMAEFHLRADTTMYNLVIRLFTEKGDM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MK+NGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKDNGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGGTCGPNTVTYTSIIQSLCE+GQPLEALKILDRMED 
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGTCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDF 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GCAPNRVTVS L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCAPNRVTVSVLVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLEERV+DGFNLCNEVDRNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVVDGFNLCNEVDRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF DQ+LVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGDQNLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. NCBI nr
Match: KAG6579716.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 871.3 bits (2250), Expect = 3.8e-249
Identity = 427/475 (89.89%), Postives = 453/475 (95.37%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCLL NVIEDY
Sbjct: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFMYTRACELIGLNRSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDI MFK+ILNLCKE KLAKEALSILGKM EFHL ADTTMYNLVIRLFTE G+M
Sbjct: 121 RREGCLVDIGMFKVILNLCKEGKLAKEALSILGKMAEFHLRADTTMYNLVIRLFTEKGDM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MK+NGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKDNGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGGTCGPNTVTYTSIIQSLCE+GQPLEALKILDRMED 
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGTCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDF 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GCAPNRVTVS L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCAPNRVTVSVLVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLE+RV+DGFNLCNEVDRNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEDRVVDGFNLCNEVDRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF DQ+LVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGDQNLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. NCBI nr
Match: XP_022928928.1 (pentatricopeptide repeat-containing protein At5g47360 [Cucurbita moschata])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 427/475 (89.89%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQK NGNVEKTLATVKTKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKKNGNVEKTLATVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVNQVLHKCS ELSQMGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCLL NVIEDY
Sbjct: 61  CVNQVLHKCSLELSQMGLRFFIWAGRQPNYRHSSFMYTRACELIGLNRSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDI MFK+ILNLCKE KLAKEALSILGKM EFHL ADTTMYNLVIRLFTE GEM
Sbjct: 121 RREGCLVDIGMFKVILNLCKEGKLAKEALSILGKMAEFHLRADTTMYNLVIRLFTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MK+NGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKDNGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGGTCGPNTVTYTSIIQSLCE+GQPLEALKILDRMED 
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGTCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDF 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GCAPNRVTVS L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCAPNRVTVSVLVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLEERV+DGFNLCNEVDRNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVVDGFNLCNEVDRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF DQ+LVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGDQNLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. NCBI nr
Match: XP_023551479.1 (pentatricopeptide repeat-containing protein At5g47360 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 868.2 bits (2242), Expect = 3.2e-248
Identity = 425/475 (89.47%), Postives = 452/475 (95.16%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTL TVKTKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLTTVKTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVN+VLHKCSFELSQMGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCL+ NVIEDY
Sbjct: 61  CVNEVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFMYTRACELIGLNRSPCLVFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCLVDI MFK+ILNLCKE KLAKEALSILG+M EFHL ADTTMYNLVIRLFTE GEM
Sbjct: 121 RREGCLVDIGMFKVILNLCKEGKLAKEALSILGEMAEFHLRADTTMYNLVIRLFTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MK+NGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKDNGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGGTCGPNTVTYTSIIQSLCE+GQPLEALKILDRMED 
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGTCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDF 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GCAPNRVTVS L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCAPNRVTVSVLVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLEERV+DGFNLCNEVDRNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVVDGFNLCNEVDRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF DQ+LVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGDQNLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. NCBI nr
Match: XP_022969835.1 (pentatricopeptide repeat-containing protein At5g47360 [Cucurbita maxima])

HSP 1 Score: 867.8 bits (2241), Expect = 4.2e-248
Identity = 425/475 (89.47%), Postives = 452/475 (95.16%), Query Frame = 0

Query: 1   MALFRISYPRSSSFRLKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSR 60
           MALFRI YPR SSFR KISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATV+TKLDSR
Sbjct: 1   MALFRIFYPRPSSFRFKISTLSTLQLSTVSSADLFYDHLQKNNGNVEKTLATVRTKLDSR 60

Query: 61  CVNQVLHKCSFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDY 120
           CVNQVLHKCSFELS MGLRFFIWAGRQPNYRHSSF+Y+RACELIG+N SPCLL NVIEDY
Sbjct: 61  CVNQVLHKCSFELSPMGLRFFIWAGRQPNYRHSSFMYARACELIGLNRSPCLLFNVIEDY 120

Query: 121 RREGCLVDIRMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEM 180
           RREGCL+DI MFK+ILNLCKE KLAKEALSILGKM EFHL ADTTMYNLVIRLFTE GEM
Sbjct: 121 RREGCLLDIGMFKVILNLCKEGKLAKEALSILGKMAEFHLRADTTMYNLVIRLFTEKGEM 180

Query: 181 DKAMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVL 240
           DKAMEL+KEMDSVDI PNMITYI++LKGFCDVGR EDAYGLFK MKENGCAPNTV YSVL
Sbjct: 181 DKAMELLKEMDSVDIDPNMITYIAMLKGFCDVGRLEDAYGLFKVMKENGCAPNTVAYSVL 240

Query: 241 LNAASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDS 300
           LN ASRHG +EKLME+LEEMEKQGG CGPNTVTYTSIIQSLCE+GQPLEALKILDRMEDS
Sbjct: 241 LNGASRHGDLEKLMELLEEMEKQGGNCGPNTVTYTSIIQSLCEVGQPLEALKILDRMEDS 300

Query: 301 GCAPNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEA 360
           GC+PNRVTVS+L+KEFCK+GH+EEAYKLIDRV ARGGASYGDCYSSLV+SL++MK+IAEA
Sbjct: 301 GCSPNRVTVSALVKEFCKDGHMEEAYKLIDRVAARGGASYGDCYSSLVISLIKMKRIAEA 360

Query: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLL 420
           EELFRNMLANGVKPDGVACTLMIKELCLEERV+DGFNLCNEV+RNGYLSSIDSDIYSLLL
Sbjct: 361 EELFRNMLANGVKPDGVACTLMIKELCLEERVVDGFNLCNEVNRNGYLSSIDSDIYSLLL 420

Query: 421 VGLFEHDHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLVMHLGGIRK 476
           VGL EHDHSVDA KLARLML+KGIRLKPHYAESI+KH+KKF  QDLVMHLGGIR+
Sbjct: 421 VGLCEHDHSVDAAKLARLMLQKGIRLKPHYAESIIKHVKKFGGQDLVMHLGGIRE 475

BLAST of Lsi09G010700 vs. TAIR 10
Match: AT5G47360.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 5.7e-118
Identity = 217/461 (47.07%), Postives = 314/461 (68.11%), Query Frame = 0

Query: 11  SSSFRLKISTLSTLQ-LSTVSSADLFYDHLQKNNGNVEKTLATVKTKLDSRCVNQVLHKC 70
           S S R + S +S L+ L+TVS+A+  Y  LQ    N+EK LA+   +LDS C+N+VL +C
Sbjct: 11  SPSLRSQPSKISALRFLTTVSAAERLYGQLQGCTSNLEKELASANVQLDSSCINEVLRRC 70

Query: 71  SFELSQMGLRFFIWAGRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDYRREGCLVDI 130
                Q GLRFFIWAG   ++RHS+++Y++AC+++ I   P L+  VIE YR+E C V++
Sbjct: 71  DPNQFQSGLRFFIWAGTLSSHRHSAYMYTKACDILKIRAKPDLIKYVIESYRKEECFVNV 130

Query: 131 RMFKIILNLCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKE 190
           +  +I+L LC +A LA EAL +L K PEF++ ADT  YNLVIRLF + G+++ A  L+KE
Sbjct: 131 KTMRIVLTLCNQANLADEALWVLRKFPEFNVCADTVAYNLVIRLFADKGDLNIADMLIKE 190

Query: 191 MDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGI 250
           MD V ++P++ITY S++ G+C+ G+ +DA+ L K M ++ C  N+V YS +L    + G 
Sbjct: 191 MDCVGLYPDVITYTSMINGYCNAGKIDDAWRLAKEMSKHDCVLNSVTYSRILEGVCKSGD 250

Query: 251 MEKLMEMLEEMEKQ--GGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRV 310
           ME+ +E+L EMEK+  GG   PN VTYT +IQ+ CE  +  EAL +LDRM + GC PNRV
Sbjct: 251 MERALELLAEMEKEDGGGLISPNAVTYTLVIQAFCEKRRVEEALLVLDRMGNRGCMPNRV 310

Query: 311 TVSSLIKEFCKNGH-VEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRN 370
           T   LI+   +N   V+   KLID++V  GG S  +C+SS  VSL+RMK+  EAE++FR 
Sbjct: 311 TACVLIQGVLENDEDVKALSKLIDKLVKLGGVSLSECFSSATVSLIRMKRWEEAEKIFRL 370

Query: 371 MLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEH 430
           ML  GV+PDG+AC+ + +ELCL ER LD F L  E+++    S+IDSDI+++LL+GL + 
Sbjct: 371 MLVRGVRPDGLACSHVFRELCLLERYLDCFLLYQEIEKKDVKSTIDSDIHAVLLLGLCQQ 430

Query: 431 DHSVDAEKLARLMLKKGIRLKPHYAESIMKHLKKFEDQDLV 468
            +S +A KLA+ ML K +RLK  + E I++ LKK  D+DL+
Sbjct: 431 GNSWEAAKLAKSMLDKKMRLKVSHVEKIIEALKKTGDEDLM 471

BLAST of Lsi09G010700 vs. TAIR 10
Match: AT1G12775.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-39
Identity = 90/313 (28.75%), Postives = 172/313 (54.95%), Query Frame = 0

Query: 135 ILN-LCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKEMDSV 194
           +LN +CK  + A  A+ +L KM E ++  D   Y+++I    ++G +D A  L  EM+  
Sbjct: 234 VLNVMCKSGQTAL-AMELLRKMEERNIKLDAVKYSIIIDGLCKDGSLDNAFNLFNEMEIK 293

Query: 195 DIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGIMEKL 254
               ++ITY +L+ GFC+ GRW+D   L + M +   +PN V +SVL+++  + G + + 
Sbjct: 294 GFKADIITYNTLIGGFCNAGRWDDGAKLLRDMIKRKISPNVVTFSVLIDSFVKEGKLREA 353

Query: 255 MEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRVTVSSLI 314
            ++L+EM ++G    PNT+TY S+I   C+  +  EA++++D M   GC P+ +T + LI
Sbjct: 354 DQLLKEMMQRG--IAPNTITYNSLIDGFCKENRLEEAIQMVDLMISKGCDPDIMTFNILI 413

Query: 315 KEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRNMLANGVK 374
             +CK   +++  +L   +  RG  +    Y++LV    +  K+  A++LF+ M++  V+
Sbjct: 414 NGYCKANRIDDGLELFREMSLRGVIANTVTYNTLVQGFCQSGKLEVAKKLFQEMVSRRVR 473

Query: 375 PDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEHDHSVDAE 434
           PD V+  +++  LC    +     +  +++++     +D  IY +++ G+       DA 
Sbjct: 474 PDIVSYKILLDGLCDNGELEKALEIFGKIEKS--KMELDIGIYMIIIHGMCNASKVDDAW 533

Query: 435 KLARLMLKKGIRL 447
            L   +  KG++L
Sbjct: 534 DLFCSLPLKGVKL 541

BLAST of Lsi09G010700 vs. TAIR 10
Match: AT1G12300.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 8.9e-39
Identity = 100/362 (27.62%), Postives = 185/362 (51.10%), Query Frame = 0

Query: 85  GRQPNYRHSSFIYSRACELIGINGSPCLLSNVIEDYRREGCLVDIRMFKIILN-LCKEAK 144
           G +P+    + + +  C L G      LL + + +Y   GC  +   +  +LN +CK  +
Sbjct: 188 GHKPDLITINTLVNGLC-LSGKEAEAMLLIDKMVEY---GCQPNAVTYGPVLNVMCKSGQ 247

Query: 145 LAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDKAMELMKEMDSVDIHPNMITYI 204
            A  A+ +L KM E ++  D   Y+++I    ++G +D A  L  EM+   I  N+ITY 
Sbjct: 248 TAL-AMELLRKMEERNIKLDAVKYSIIIDGLCKHGSLDNAFNLFNEMEMKGITTNIITYN 307

Query: 205 SLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLNAASRHGIMEKLMEMLEEMEKQ 264
            L+ GFC+ GRW+D   L + M +    PN V +SVL+++  + G + +  E+ +EM  +
Sbjct: 308 ILIGGFCNAGRWDDGAKLLRDMIKRKINPNVVTFSVLIDSFVKEGKLREAEELHKEMIHR 367

Query: 265 GGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGCAPNRVTVSSLIKEFCKNGHVE 324
           G    P+T+TYTS+I   C+     +A +++D M   GC PN  T + LI  +CK   ++
Sbjct: 368 G--IAPDTITYTSLIDGFCKENHLDKANQMVDLMVSKGCDPNIRTFNILINGYCKANRID 427

Query: 325 EAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEELFRNMLANGVKPDGVACTLMI 384
           +  +L  ++  RG  +    Y++L+     + K+  A+ELF+ M++  V P+ V   +++
Sbjct: 428 DGLELFRKMSLRGVVADTVTYNTLIQGFCELGKLNVAKELFQEMVSRKVPPNIVTYKILL 487

Query: 385 KELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVGLFEHDHSVDAEKLARLMLKKG 444
             LC          +  +++++     +D  IY++++ G+       DA  L   +  KG
Sbjct: 488 DGLCDNGESEKALEIFEKIEKS--KMELDIGIYNIIIHGMCNASKVDDAWDLFCSLPLKG 540

Query: 445 IR 446
           ++
Sbjct: 548 VK 540

BLAST of Lsi09G010700 vs. TAIR 10
Match: AT1G09680.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 3.4e-38
Identity = 89/322 (27.64%), Postives = 169/322 (52.48%), Query Frame = 0

Query: 124 GCLVDIRMFKIILN-LCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDK 183
           G  +++ +F I++N  CKE  ++ +A  +  ++ +  L      +N +I  + + G +D+
Sbjct: 235 GFPLNVYVFNILMNKFCKEGNIS-DAQKVFDEITKRSLQPTVVSFNTLINGYCKVGNLDE 294

Query: 184 AMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLN 243
              L  +M+     P++ TY +L+   C   + + A+GLF  M + G  PN V+++ L++
Sbjct: 295 GFRLKHQMEKSRTRPDVFTYSALINALCKENKMDGAHGLFDEMCKRGLIPNDVIFTTLIH 354

Query: 244 AASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGC 303
             SR+G ++ + E  ++M  +G    P+ V Y +++   C+ G  + A  I+D M   G 
Sbjct: 355 GHSRNGEIDLMKESYQKMLSKG--LQPDIVLYNTLVNGFCKNGDLVAARNIVDGMIRRGL 414

Query: 304 APNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEE 363
            P+++T ++LI  FC+ G VE A ++   +   G       +S+LV  + +  ++ +AE 
Sbjct: 415 RPDKITYTTLIDGFCRGGDVETALEIRKEMDQNGIELDRVGFSALVCGMCKEGRVIDAER 474

Query: 364 LFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDIYSLLLVG 423
             R ML  G+KPD V  T+M+   C +     GF L  E+  +G++ S+ +  Y++LL G
Sbjct: 475 ALREMLRAGIKPDDVTYTMMMDAFCKKGDAQTGFKLLKEMQSDGHVPSVVT--YNVLLNG 534

Query: 424 LFEHDHSVDAEKLARLMLKKGI 445
           L +     +A+ L   ML  G+
Sbjct: 535 LCKLGQMKNADMLLDAMLNIGV 551

BLAST of Lsi09G010700 vs. TAIR 10
Match: AT1G12700.1 (ATP binding;nucleic acid binding;helicases )

HSP 1 Score: 156.8 bits (395), Expect = 4.4e-38
Identity = 97/325 (29.85%), Postives = 170/325 (52.31%), Query Frame = 0

Query: 124 GCLVDIRMFKIILN-LCKEAKLAKEALSILGKMPEFHLHADTTMYNLVIRLFTENGEMDK 183
           GC  D+  +  I+N +C+    +  AL +L KM E ++ AD   Y+ +I     +G +D 
Sbjct: 188 GCQPDVVTYNSIVNGICRSGDTSL-ALDLLRKMEERNVKADVFTYSTIIDSLCRDGCIDA 247

Query: 184 AMELMKEMDSVDIHPNMITYISLLKGFCDVGRWEDAYGLFKAMKENGCAPNTVVYSVLLN 243
           A+ L KEM++  I  +++TY SL++G C  G+W D   L K M      PN + ++VLL+
Sbjct: 248 AISLFKEMETKGIKSSVVTYNSLVRGLCKAGKWNDGALLLKDMVSREIVPNVITFNVLLD 307

Query: 244 AASRHGIMEKLMEMLEEMEKQGGTCGPNTVTYTSIIQSLCELGQPLEALKILDRMEDSGC 303
              + G +++  E+ +EM  +G    PN +TY +++   C   +  EA  +LD M  + C
Sbjct: 308 VFVKEGKLQEANELYKEMITRG--ISPNIITYNTLMDGYCMQNRLSEANNMLDLMVRNKC 367

Query: 304 APNRVTVSSLIKEFCKNGHVEEAYKLIDRVVARGGASYGDCYSSLVVSLVRMKKIAEAEE 363
           +P+ VT +SLIK +C    V++  K+   +  RG  +    YS LV    +  KI  AEE
Sbjct: 368 SPDIVTFTSLIKGYCMVKRVDDGMKVFRNISKRGLVANAVTYSILVQGFCQSGKIKLAEE 427

Query: 364 LFRNMLANGVKPDGVACTLMIKELCLEERVLDGFNLCNEVDRNGYLSSIDSDI--YSLLL 423
           LF+ M+++GV PD +   +++  LC   ++     +  ++ +    S +D  I  Y+ ++
Sbjct: 428 LFQEMVSHGVLPDVMTYGILLDGLCDNGKLEKALEIFEDLQK----SKMDLGIVMYTTII 487

Query: 424 VGLFEHDHSVDAEKLARLMLKKGIR 446
            G+ +     DA  L   +  KG++
Sbjct: 488 EGMCKGGKVEDAWNLFCSLPCKGVK 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVS38.0e-11747.07Pentatricopeptide repeat-containing protein At5g47360 OS=Arabidopsis thaliana OX... [more]
Q9LPX21.9e-3828.75Pentatricopeptide repeat-containing protein At1g12775, mitochondrial OS=Arabidop... [more]
Q0WKV31.3e-3727.62Pentatricopeptide repeat-containing protein At1g12300, mitochondrial OS=Arabidop... [more]
O044914.8e-3727.64Putative pentatricopeptide repeat-containing protein At1g09680 OS=Arabidopsis th... [more]
P0C7Q76.3e-3729.85Putative pentatricopeptide repeat-containing protein At1g12700, mitochondrial OS... [more]
Match NameE-valueIdentityDescription
A0A6J1EQI29.1e-24989.89pentatricopeptide repeat-containing protein At5g47360 OS=Cucurbita moschata OX=3... [more]
A0A6J1I1252.0e-24889.47pentatricopeptide repeat-containing protein At5g47360 OS=Cucurbita maxima OX=366... [more]
A0A0A0LI441.4e-24188.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G123590 PE=4 SV=1[more]
A0A1S3B4L95.7e-23586.11pentatricopeptide repeat-containing protein At5g47360 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7SSI75.7e-23586.11Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
KAG7017159.11.7e-24990.11Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
KAG6579716.13.8e-24989.89Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_022928928.11.9e-24889.89pentatricopeptide repeat-containing protein At5g47360 [Cucurbita moschata][more]
XP_023551479.13.2e-24889.47pentatricopeptide repeat-containing protein At5g47360 [Cucurbita pepo subsp. pep... [more]
XP_022969835.14.2e-24889.47pentatricopeptide repeat-containing protein At5g47360 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G47360.15.7e-11847.07Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G12775.11.4e-3928.75Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G12300.18.9e-3927.62Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G09680.13.4e-3827.64Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G12700.14.4e-3829.85ATP binding;nucleic acid binding;helicases [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 235..264
e-value: 9.1E-4
score: 17.2
coord: 200..234
e-value: 3.1E-9
score: 34.4
coord: 166..198
e-value: 2.1E-6
score: 25.5
coord: 307..336
e-value: 0.001
score: 17.1
coord: 343..375
e-value: 1.8E-6
score: 25.7
coord: 272..305
e-value: 3.5E-9
score: 34.3
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 343..372
e-value: 0.0042
score: 17.2
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 269..318
e-value: 2.0E-14
score: 53.5
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 270..304
score: 13.109773
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 305..339
score: 9.854266
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 340..374
score: 9.920034
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 233..267
score: 9.481582
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 198..232
score: 13.285153
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 163..197
score: 11.158661
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 263..333
e-value: 1.9E-20
score: 75.2
coord: 35..192
e-value: 1.0E-17
score: 66.2
coord: 193..262
e-value: 6.6E-20
score: 73.4
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 338..470
e-value: 1.1E-14
score: 56.6
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 270..369
IPR033443Pentacotripeptide-repeat region of PRORPPFAMPF17177PPR_longcoord: 119..263
e-value: 3.6E-15
score: 55.9
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 19..471
NoneNo IPR availablePANTHERPTHR45613:SF82PPR CONTAINING PLANT-LIKE PROTEINcoord: 19..471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G010700.1Lsi09G010700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding