Lsi09G010070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G010070
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionthioredoxin-like fold domain-containing protein MRL7L, chloroplastic
Locationchr09: 13755009 .. 13756221 (+)
RNA-Seq ExpressionLsi09G010070
SyntenyLsi09G010070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTGTATTATGCTATGATTTTATTCTAAACCCATTTCTCAAACAAATAGGTGAGTTCGTAATTTGTGCATCTTTGTAGAAATGGCGCTGCAACATACACTAGGTTTACTGTCTCTGGTTTTGCCTTCTTCTAATTGCAAATTCGAAGGGGTTGCTTTATTTGCGCCAACGAATTCCCAAACCAGCAACGCTCTCTCTTCATGGAGGTTGGAGTCTTCTTATCCTTCATCCATCTCTTTGATGAAGTTCCCTTTCCTGGTAATGTCATTCTCTAATCCATTTTCTTATAGTGTGTTTGGCATATGGTATAACTTCTTTACTGATTTTAGATGCTTAGAAGTGTTTTACCTAAGATTTGTAGTTGATTTCAAAACTTCTCATAAGAAAGATTTGAAAGCTGATTCTCCAAGTGATTTTAGAGTGGTTGACTGGGAATCATTTTTTCACTTTATGGTAATTTGATATTTATATTTTAGACATGGTGCAACAACATATTTGTAAATAAATCACTTTAAGAGAATGTAAATAAACAAATAATTTTTTGAAACAGTGCTTAAACTAAAATGTATGCCCAAGTCACCCTTAGTGTTCGTTTCTTGTTACCTGGGGAAACTAACTGTGAAGCACTTCATGTTATGATCTGTAGGGTAGTTGTTTTAGAAGCAGCACTCTAAGGGCCTATAAAACTGCAGGGTCACTCAGGTCTGGTGATGGAAAGAAGAAGAAAGGACAAGCAGATATGGACTCTGATGAGGAAGATGATTCTTCTTCAACAAAGAAGCAAGACCTTGAGCTTATGGATGCCGAGGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCAATGTTGAGGAGGAGATAGACAACATGGAAAGGAGAAGGAAAATGCAAAAACTTCTTGCTGACTATCCTCTTGTTGTGGAGGAGGAGGATCCTGATTGGCCTGAGGATGCTGACGGTTGGGGTTTCAACTTGGGTCAGTTTTTTGATAAGATCACCATCAAGAATAAGAAAAAGGATGATGATAAATATGATGACGATAAAGATGACACTGGTGATGAAATAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAACAATTGCTGAATGGGAGGAGGCCGTATTTAAAGATATTAGTCCTCTAATCATTTTTGTACATAATCGCTACAAAAGGTATGCTCTTTGA

mRNA sequence

GGTTGTATTATGCTATGATTTTATTCTAAACCCATTTCTCAAACAAATAGGTGAGTTCGTAATTTGTGCATCTTTGTAGAAATGGCGCTGCAACATACACTAGGTTTACTGTCTCTGGTTTTGCCTTCTTCTAATTGCAAATTCGAAGGGGTTGCTTTATTTGCGCCAACGAATTCCCAAACCAGCAACGCTCTCTCTTCATGGAGGTTGGAGTCTTCTTATCCTTCATCCATCTCTTTGATGAAGTTCCCTTTCCTGGGTAGTTGTTTTAGAAGCAGCACTCTAAGGGCCTATAAAACTGCAGGGTCACTCAGGTCTGGTGATGGAAAGAAGAAGAAAGGACAAGCAGATATGGACTCTGATGAGGAAGATGATTCTTCTTCAACAAAGAAGCAAGACCTTGAGCTTATGGATGCCGAGGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCAATGTTGAGGAGGAGATAGACAACATGGAAAGGAGAAGGAAAATGCAAAAACTTCTTGCTGACTATCCTCTTGTTGTGGAGGAGGAGGATCCTGATTGGCCTGAGGATGCTGACGGTTGGGGTTTCAACTTGGGTCAGTTTTTTGATAAGATCACCATCAAGAATAAGAAAAAGGATGATGATAAATATGATGACGATAAAGATGACACTGGTGATGAAATAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAACAATTGCTGAATGGGAGGAGGCCGTATTTAAAGATATTAGTCCTCTAATCATTTTTGTACATAATCGCTACAAAAGGTATGCTCTTTGA

Coding sequence (CDS)

ATGGCGCTGCAACATACACTAGGTTTACTGTCTCTGGTTTTGCCTTCTTCTAATTGCAAATTCGAAGGGGTTGCTTTATTTGCGCCAACGAATTCCCAAACCAGCAACGCTCTCTCTTCATGGAGGTTGGAGTCTTCTTATCCTTCATCCATCTCTTTGATGAAGTTCCCTTTCCTGGGTAGTTGTTTTAGAAGCAGCACTCTAAGGGCCTATAAAACTGCAGGGTCACTCAGGTCTGGTGATGGAAAGAAGAAGAAAGGACAAGCAGATATGGACTCTGATGAGGAAGATGATTCTTCTTCAACAAAGAAGCAAGACCTTGAGCTTATGGATGCCGAGGAGAGACGGGAATGGAGGGAGAAGATTAGGGAAGTGATTGATATGAACCCCAATGTTGAGGAGGAGATAGACAACATGGAAAGGAGAAGGAAAATGCAAAAACTTCTTGCTGACTATCCTCTTGTTGTGGAGGAGGAGGATCCTGATTGGCCTGAGGATGCTGACGGTTGGGGTTTCAACTTGGGTCAGTTTTTTGATAAGATCACCATCAAGAATAAGAAAAAGGATGATGATAAATATGATGACGATAAAGATGACACTGGTGATGAAATAGTTTGGCAAGATGACAACTACATTCGTCCAATTAAGGACATAACAATTGCTGAATGGGAGGAGGCCGTATTTAAAGATATTAGTCCTCTAATCATTTTTGTACATAATCGCTACAAAAGGTATGCTCTTTGA

Protein sequence

MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLGSCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWREKIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDKITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNRYKRYAL
Homology
BLAST of Lsi09G010070 vs. ExPASy Swiss-Prot
Match: Q9SKB6 (Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7L PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.6e-52
Identity = 112/172 (65.12%), Postives = 134/172 (77.91%), Query Frame = 0

Query: 82  GKKKKGQADMDSDEEDDSSSTKKQ-----DLELMDAEERREWREKIREVIDMNPNVE--E 141
           GKK   + + DSD ED+    K++     +  L D  ERREWR+ IREVID +P++E  E
Sbjct: 72  GKKVWRKKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDE 131

Query: 142 EIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDKITIKNKKK--DDDK 201
           EID +E+RRKMQKLLADYPLVV EEDP+WPEDADGWGF+  QFF+KITIKN+KK  +DD 
Sbjct: 132 EIDMVEKRRKMQKLLADYPLVVNEEDPNWPEDADGWGFSFNQFFNKITIKNEKKEEEDDD 191

Query: 202 YDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNRYKR 245
            D + DD+  EIVWQDDNYIRPIKD+T AEWEEAVFKDISPL++ VHNRYKR
Sbjct: 192 EDSEGDDSEKEIVWQDDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKR 243

BLAST of Lsi09G010070 vs. ExPASy Swiss-Prot
Match: Q0DI48 (Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7L PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.8e-35
Identity = 89/168 (52.98%), Postives = 109/168 (64.88%), Query Frame = 0

Query: 80  GDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWREKIREVIDMNPNVEEEIDNM 139
           G   +  G     SD+EDD    +  D      E+RR  R KIRE++D  P   E  D  
Sbjct: 64  GGRARDDGSESESSDDEDDDEPMQMTD------EQRRTLRRKIREMMDRVPETAEITDPA 123

Query: 140 ERRRKMQKLLADYPLVVEEEDPDWPE-DADGWGFNLGQFFDKITIK-NKKKDDDKYDDDK 199
           ER+ KM +LL  Y LVVEEEDP+WPE D DG GF+LGQFFDKITIK  KK DDD+ DD K
Sbjct: 124 ERKAKMLELLTKYQLVVEEEDPNWPEDDEDGHGFSLGQFFDKITIKAEKKNDDDEEDDAK 183

Query: 200 DDTGD-EIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNRYKR 245
            +  D EIVW+DDNYI+PI+D+   +W++ VF D  PLI+ VHNRYKR
Sbjct: 184 GNQSDKEIVWEDDNYIKPIRDVKTMDWDDTVFTDFGPLIVLVHNRYKR 225

BLAST of Lsi09G010070 vs. ExPASy Swiss-Prot
Match: Q5QLS7 (Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=MRL7 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.9e-11
Identity = 48/132 (36.36%), Postives = 67/132 (50.76%), Query Frame = 0

Query: 119 REKIREVIDMNPNV---EEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDAD-GWGFNL 178
           RE+  EV++   ++   +EE+ + E +    +   +   VVEEEDP WP DAD GWG   
Sbjct: 101 RERDPEVLEGKGDILKRKEELSDEEGKEGTGEEEGELKAVVEEEDPSWPLDADIGWGIRA 160

Query: 179 GQFFDKITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPL 238
            ++FDK +IKN   D           G EI W+ +     +K+I   EWE   F   SPL
Sbjct: 161 SEYFDKHSIKNVTVD-----------GVEIDWEREVEEGWVKEINCLEWESFAFHP-SPL 220

Query: 239 IIFVHNRYKRYA 247
           I+ V  RY R A
Sbjct: 221 IVLVFERYNRAA 220

BLAST of Lsi09G010070 vs. ExPASy Swiss-Prot
Match: F4JLC1 (Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MRL7 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.1e-08
Identity = 51/163 (31.29%), Postives = 72/163 (44.17%), Query Frame = 0

Query: 83  KKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWREKIREVIDMNPNVEEEIDNMERR 142
           K K+G+       ED   S+ ++    +  +    +  K +     +  V+EE +  E  
Sbjct: 93  KSKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKANFIKDRGVDEEEEEEEE- 152

Query: 143 RKMQKLLADYPLVVEEEDPDWPEDAD-GWGFNLGQFFDKITIKNKKKDDDKYDDDKDDTG 202
                      +VVEEEDPDWP D D GWG    ++FD   IKN   DD          G
Sbjct: 153 -----------MVVEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDD----------G 212

Query: 203 DEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNRYKR 245
            EI W+ +     +K+I   EWE   F   SPL++ V  RYKR
Sbjct: 213 SEIDWEGEIDDSWVKEINCLEWESFAFHP-SPLVVLVFERYKR 232

BLAST of Lsi09G010070 vs. ExPASy TrEMBL
Match: A0A5D3BBT5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1159G00150 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 3.2e-112
Identity = 213/244 (87.30%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQHTLGLLSL LPSSNCKFEG ALF  T+S T+N LSSW LESSYPSSIS MKFPF G
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGALFVSTSSHTTNLLSSWTLESSYPSSISFMKFPFRG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
           SCFRSSTLRAY+TAGS++ GDGKKKKGQA MDSDEEDDSSS K+ D E MDAEER+EWRE
Sbjct: 61  SCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIR+VIDMNPNVEEEIDNMER+RKMQKLLA+YPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           ITIKNKKK D+K+DDDKDDT +EIVWQDDNYIRPIKDITI+EWEEAVFKDISPLIIFVHN
Sbjct: 181 ITIKNKKK-DNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. ExPASy TrEMBL
Match: A0A1S3B4P3 (uncharacterized protein LOC103485750 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485750 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 3.2e-112
Identity = 213/244 (87.30%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQHTLGLLSL LPSSNCKFEG ALF  T+S T+N LSSW LESSYPSSIS MKFPF G
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGALFVSTSSHTTNLLSSWTLESSYPSSISFMKFPFRG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
           SCFRSSTLRAY+TAGS++ GDGKKKKGQA MDSDEEDDSSS K+ D E MDAEER+EWRE
Sbjct: 61  SCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIR+VIDMNPNVEEEIDNMER+RKMQKLLA+YPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           ITIKNKKK D+K+DDDKDDT +EIVWQDDNYIRPIKDITI+EWEEAVFKDISPLIIFVHN
Sbjct: 181 ITIKNKKK-DNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. ExPASy TrEMBL
Match: A0A0A0LK68 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G123060 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 4.3e-109
Identity = 208/244 (85.25%), Postives = 222/244 (90.98%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQH+LGL SL+LPSSNCKFEG A F  T+SQ +N  SSW L+SSYPSSIS M FPF G
Sbjct: 1   MALQHSLGLPSLLLPSSNCKFEGGASFVSTSSQITNLFSSWTLDSSYPSSISFMNFPFRG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
            CFRSS+LRAY+TAGS+R GDGKKKK QA MDSDEEDDSSS K+ D+ELMDAEERREWRE
Sbjct: 61  GCFRSSSLRAYRTAGSIRPGDGKKKKEQAGMDSDEEDDSSSAKEPDVELMDAEERREWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIR+VID NPNVEEEIDNMERR KMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDTNPNVEEEIDNMERRIKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           ITIKNKKK DDKYDDDKDDT +E+VWQDDNYIRPIKDITI+EWEEAVFKDISPLIIFVHN
Sbjct: 181 ITIKNKKK-DDKYDDDKDDTDNEVVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. ExPASy TrEMBL
Match: A0A6J1DK13 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021817 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.1e-104
Identity = 203/244 (83.20%), Postives = 219/244 (89.75%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQH LG   LV+P ++ KF G ALFA TNSQT+  L +WRL+SSYPSSISL+K PFLG
Sbjct: 1   MALQHPLGFPFLVMPCAHYKFRGGALFASTNSQTNKFLYTWRLKSSYPSSISLIKPPFLG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
           SCFRSSTLRAYKTAG LRS DGKKKK QADMDSDEEDDSSS  +QD +LMD++ER EWR+
Sbjct: 61  SCFRSSTLRAYKTAGPLRSDDGKKKKVQADMDSDEEDDSSSEDRQDPDLMDSDERWEWRK 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIREVID NPNVEEEIDNMERRRKMQKLLA+YPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIREVIDRNPNVEEEIDNMERRRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           I+IKNKKK DDKYDDDKDD+ +EIVWQDDNYIRPIKDIT AEWEEAVFKDISPLII VHN
Sbjct: 181 ISIKNKKK-DDKYDDDKDDSDNEIVWQDDNYIRPIKDITTAEWEEAVFKDISPLIILVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. ExPASy TrEMBL
Match: A0A6J1HZ67 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468989 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.6e-103
Identity = 203/245 (82.86%), Postives = 217/245 (88.57%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVAL-FAPTNSQTSNALSSWRLESSYPSSISLMKFPFL 60
           MALQHTL L  LVLPSSNC+F+G A  FA TNSQ S+A +SWRL+SSYPSS++ +K  FL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSVASVKLSFL 60

Query: 61  GSCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWR 120
           GS  RSST  AYKTAGSL SGDGKKKKGQADMDSDEEDDSSS K+QDLELMD EERREWR
Sbjct: 61  GSFLRSSTRGAYKTAGSLWSGDGKKKKGQADMDSDEEDDSSSAKQQDLELMDVEERREWR 120

Query: 121 EKIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFD 180
           EKIREVIDMNP+VEEE+D MERRRKMQKLLADYPLV+EEEDP+WPEDADGWGFNLGQFFD
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVMEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVH 240
           KITIKNKKKDD   DDDKDD  DE+VWQDDNYIRPIKDI  AEWEEAVFKDISPLII VH
Sbjct: 181 KITIKNKKKDDKDDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 NRYKR 245
           NRYKR
Sbjct: 241 NRYKR 245

BLAST of Lsi09G010070 vs. NCBI nr
Match: XP_038876051.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida] >XP_038876052.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida] >XP_038876053.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida] >XP_038876054.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa hispida])

HSP 1 Score: 427.9 bits (1099), Expect = 5.8e-116
Identity = 223/244 (91.39%), Postives = 229/244 (93.85%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQHTLGL SLVLPSSNCKFEG  LF PTNSQTS+ LSSW+LE SY SSISLMKFP  G
Sbjct: 1   MALQHTLGLPSLVLPSSNCKFEGGTLFVPTNSQTSSVLSSWKLECSYASSISLMKFPSQG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
           SCFRSSTLRAYKTAGSLRSGDGKKKKGQ DMDSDEEDDSSS K+QDLELMDAEERREWRE
Sbjct: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQVDMDSDEEDDSSSEKQQDLELMDAEERREWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIREVIDMNPNVEEEIDNME+RR MQKLLADYPLVV+EEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIREVIDMNPNVEEEIDNMEKRRMMQKLLADYPLVVDEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           ITIKNKKK DDK DDDKDDT +EIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN
Sbjct: 181 ITIKNKKK-DDKDDDDKDDTDNEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. NCBI nr
Match: XP_008441666.1 (PREDICTED: uncharacterized protein LOC103485750 isoform X1 [Cucumis melo] >KAA0032393.1 uncharacterized protein E6C27_scaffold219G002690 [Cucumis melo var. makuwa] >TYJ96356.1 uncharacterized protein E5676_scaffold1159G00150 [Cucumis melo var. makuwa])

HSP 1 Score: 414.5 bits (1064), Expect = 6.6e-112
Identity = 213/244 (87.30%), Postives = 227/244 (93.03%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQHTLGLLSL LPSSNCKFEG ALF  T+S T+N LSSW LESSYPSSIS MKFPF G
Sbjct: 1   MALQHTLGLLSLALPSSNCKFEGGALFVSTSSHTTNLLSSWTLESSYPSSISFMKFPFRG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
           SCFRSSTLRAY+TAGS++ GDGKKKKGQA MDSDEEDDSSS K+ D E MDAEER+EWRE
Sbjct: 61  SCFRSSTLRAYRTAGSIKPGDGKKKKGQAGMDSDEEDDSSSAKQLDAEPMDAEERQEWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIR+VIDMNPNVEEEIDNMER+RKMQKLLA+YPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDMNPNVEEEIDNMERKRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           ITIKNKKK D+K+DDDKDDT +EIVWQDDNYIRPIKDITI+EWEEAVFKDISPLIIFVHN
Sbjct: 181 ITIKNKKK-DNKFDDDKDDTDNEIVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. NCBI nr
Match: XP_004138945.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucumis sativus] >XP_011649078.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucumis sativus] >KGN61444.1 hypothetical protein Csa_006009 [Cucumis sativus])

HSP 1 Score: 404.1 bits (1037), Expect = 8.9e-109
Identity = 208/244 (85.25%), Postives = 222/244 (90.98%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQH+LGL SL+LPSSNCKFEG A F  T+SQ +N  SSW L+SSYPSSIS M FPF G
Sbjct: 1   MALQHSLGLPSLLLPSSNCKFEGGASFVSTSSQITNLFSSWTLDSSYPSSISFMNFPFRG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
            CFRSS+LRAY+TAGS+R GDGKKKK QA MDSDEEDDSSS K+ D+ELMDAEERREWRE
Sbjct: 61  GCFRSSSLRAYRTAGSIRPGDGKKKKEQAGMDSDEEDDSSSAKEPDVELMDAEERREWRE 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIR+VID NPNVEEEIDNMERR KMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIRKVIDTNPNVEEEIDNMERRIKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           ITIKNKKK DDKYDDDKDDT +E+VWQDDNYIRPIKDITI+EWEEAVFKDISPLIIFVHN
Sbjct: 181 ITIKNKKK-DDKYDDDKDDTDNEVVWQDDNYIRPIKDITISEWEEAVFKDISPLIIFVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. NCBI nr
Match: XP_022154590.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 386.7 bits (992), Expect = 1.5e-103
Identity = 203/244 (83.20%), Postives = 219/244 (89.75%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVALFAPTNSQTSNALSSWRLESSYPSSISLMKFPFLG 60
           MALQH LG   LV+P ++ KF G ALFA TNSQT+  L +WRL+SSYPSSISL+K PFLG
Sbjct: 1   MALQHPLGFPFLVMPCAHYKFRGGALFASTNSQTNKFLYTWRLKSSYPSSISLIKPPFLG 60

Query: 61  SCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWRE 120
           SCFRSSTLRAYKTAG LRS DGKKKK QADMDSDEEDDSSS  +QD +LMD++ER EWR+
Sbjct: 61  SCFRSSTLRAYKTAGPLRSDDGKKKKVQADMDSDEEDDSSSEDRQDPDLMDSDERWEWRK 120

Query: 121 KIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180
           KIREVID NPNVEEEIDNMERRRKMQKLLA+YPLVVEEEDPDWPEDADGWGFNLGQFFDK
Sbjct: 121 KIREVIDRNPNVEEEIDNMERRRKMQKLLAEYPLVVEEEDPDWPEDADGWGFNLGQFFDK 180

Query: 181 ITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHN 240
           I+IKNKKK DDKYDDDKDD+ +EIVWQDDNYIRPIKDIT AEWEEAVFKDISPLII VHN
Sbjct: 181 ISIKNKKK-DDKYDDDKDDSDNEIVWQDDNYIRPIKDITTAEWEEAVFKDISPLIILVHN 240

Query: 241 RYKR 245
           RYKR
Sbjct: 241 RYKR 243

BLAST of Lsi09G010070 vs. NCBI nr
Match: XP_022969940.1 (thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 [Cucurbita maxima] >XP_022969941.1 thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 385.6 bits (989), Expect = 3.3e-103
Identity = 203/245 (82.86%), Postives = 217/245 (88.57%), Query Frame = 0

Query: 1   MALQHTLGLLSLVLPSSNCKFEGVAL-FAPTNSQTSNALSSWRLESSYPSSISLMKFPFL 60
           MALQHTL L  LVLPSSNC+F+G A  FA TNSQ S+A +SWRL+SSYPSS++ +K  FL
Sbjct: 1   MALQHTLRLPFLVLPSSNCRFQGGAFAFAATNSQPSDAFASWRLKSSYPSSVASVKLSFL 60

Query: 61  GSCFRSSTLRAYKTAGSLRSGDGKKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWR 120
           GS  RSST  AYKTAGSL SGDGKKKKGQADMDSDEEDDSSS K+QDLELMD EERREWR
Sbjct: 61  GSFLRSSTRGAYKTAGSLWSGDGKKKKGQADMDSDEEDDSSSAKQQDLELMDVEERREWR 120

Query: 121 EKIREVIDMNPNVEEEIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFD 180
           EKIREVIDMNP+VEEE+D MERRRKMQKLLADYPLV+EEEDP+WPEDADGWGFNLGQFFD
Sbjct: 121 EKIREVIDMNPDVEEEMDIMERRRKMQKLLADYPLVMEEEDPNWPEDADGWGFNLGQFFD 180

Query: 181 KITIKNKKKDDDKYDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVH 240
           KITIKNKKKDD   DDDKDD  DE+VWQDDNYIRPIKDI  AEWEEAVFKDISPLII VH
Sbjct: 181 KITIKNKKKDDKDDDDDKDDNDDEVVWQDDNYIRPIKDIATAEWEEAVFKDISPLIILVH 240

Query: 241 NRYKR 245
           NRYKR
Sbjct: 241 NRYKR 245

BLAST of Lsi09G010070 vs. TAIR 10
Match: AT2G31840.1 (Thioredoxin superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.1e-53
Identity = 112/172 (65.12%), Postives = 134/172 (77.91%), Query Frame = 0

Query: 82  GKKKKGQADMDSDEEDDSSSTKKQ-----DLELMDAEERREWREKIREVIDMNPNVE--E 141
           GKK   + + DSD ED+    K++     +  L D  ERREWR+ IREVID +P++E  E
Sbjct: 72  GKKVWRKKEEDSDSEDEEDEVKEETFGGKEASLDDPVERREWRKTIREVIDKHPDIEEDE 131

Query: 142 EIDNMERRRKMQKLLADYPLVVEEEDPDWPEDADGWGFNLGQFFDKITIKNKKK--DDDK 201
           EID +E+RRKMQKLLADYPLVV EEDP+WPEDADGWGF+  QFF+KITIKN+KK  +DD 
Sbjct: 132 EIDMVEKRRKMQKLLADYPLVVNEEDPNWPEDADGWGFSFNQFFNKITIKNEKKEEEDDD 191

Query: 202 YDDDKDDTGDEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNRYKR 245
            D + DD+  EIVWQDDNYIRPIKD+T AEWEEAVFKDISPL++ VHNRYKR
Sbjct: 192 EDSEGDDSEKEIVWQDDNYIRPIKDLTTAEWEEAVFKDISPLMVLVHNRYKR 243

BLAST of Lsi09G010070 vs. TAIR 10
Match: AT4G28590.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: Thioredoxin superfamily protein (TAIR:AT2G31840.1); Has 114 Blast hits to 112 proteins in 39 species: Archae - 2; Bacteria - 0; Metazoa - 17; Fungi - 6; Plants - 67; Viruses - 0; Other Eukaryotes - 22 (source: NCBI BLink). )

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-09
Identity = 51/163 (31.29%), Postives = 72/163 (44.17%), Query Frame = 0

Query: 83  KKKKGQADMDSDEEDDSSSTKKQDLELMDAEERREWREKIREVIDMNPNVEEEIDNMERR 142
           K K+G+       ED   S+ ++    +  +    +  K +     +  V+EE +  E  
Sbjct: 93  KSKRGRRSEADAVEDYVRSSLERTFSTIKEQNPEVFENKEKANFIKDRGVDEEEEEEEE- 152

Query: 143 RKMQKLLADYPLVVEEEDPDWPEDAD-GWGFNLGQFFDKITIKNKKKDDDKYDDDKDDTG 202
                      +VVEEEDPDWP D D GWG    ++FD   IKN   DD          G
Sbjct: 153 -----------MVVEEEDPDWPVDTDVGWGIKASEYFDTHPIKNVVGDD----------G 212

Query: 203 DEIVWQDDNYIRPIKDITIAEWEEAVFKDISPLIIFVHNRYKR 245
            EI W+ +     +K+I   EWE   F   SPL++ V  RYKR
Sbjct: 213 SEIDWEGEIDDSWVKEINCLEWESFAFHP-SPLVVLVFERYKR 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SKB61.6e-5265.12Thioredoxin-like fold domain-containing protein MRL7L, chloroplastic OS=Arabidop... [more]
Q0DI481.8e-3552.98Thioredoxin-like fold domain-containing protein MRL7L homolog, chloroplastic OS=... [more]
Q5QLS78.9e-1136.36Thioredoxin-like fold domain-containing protein MRL7 homolog, chloroplastic OS=O... [more]
F4JLC14.1e-0831.29Thioredoxin-like fold domain-containing protein MRL7, chloroplastic OS=Arabidops... [more]
Match NameE-valueIdentityDescription
A0A5D3BBT53.2e-11287.30Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B4P33.2e-11287.30uncharacterized protein LOC103485750 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LK684.3e-10985.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G123060 PE=4 SV=1[more]
A0A6J1DK137.1e-10483.20thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 ... [more]
A0A6J1HZ671.6e-10382.86thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 ... [more]
Match NameE-valueIdentityDescription
XP_038876051.15.8e-11691.39thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Benincasa ... [more]
XP_008441666.16.6e-11287.30PREDICTED: uncharacterized protein LOC103485750 isoform X1 [Cucumis melo] >KAA00... [more]
XP_004138945.18.9e-10985.25thioredoxin-like fold domain-containing protein MRL7L, chloroplastic [Cucumis sa... [more]
XP_022154590.11.5e-10383.20thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X2 ... [more]
XP_022969940.13.3e-10382.86thioredoxin-like fold domain-containing protein MRL7L, chloroplastic isoform X1 ... [more]
Match NameE-valueIdentityDescription
AT2G31840.11.1e-5365.12Thioredoxin superfamily protein [more]
AT4G28590.12.9e-0931.29FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..113
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..113
NoneNo IPR availablePANTHERPTHR34669:SF1THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 1..244
IPR044701Thioredoxin-like fold domain-containing protein MRL7/MRL7LPANTHERPTHR34669THIOREDOXIN-LIKE FOLD DOMAIN-CONTAINING PROTEIN MRL7L, CHLOROPLASTICcoord: 1..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G010070.1Lsi09G010070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009570 chloroplast stroma