Lsi09G009870 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G009870
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNitrate reductase
Locationchr09: 13311405 .. 13314631 (-)
RNA-Seq ExpressionLsi09G009870
SyntenyLsi09G009870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCTTCCGTCGACAACCGCCAATTTGGATCTCTTCAGCCACCGCTTAACGGCGTCGTGCGCTCCTTCAAGTCCGGTTCTAACCACCGGTCCGACTCGCCGGTTCGTGGCTGTAACTTCGATTACAATAATAACAACCGCCTTCTCAAAAGTTCCGTGAAGATAGAACAGGAAGAGGAGGAGGATTCGTGTAGTGAAGACGAGAATGATAACTACGAGTTACGTGAGTTGATTCTGAAGGGAAAGCGTGAACTGGAGCCGTCGATTGTGGACCACAGAGATGAAGGGACGGCTGATAATTGGATCCAGCGGAACCCTTCCATGGTCCGTCTCACAGGGAAGCACCCCTTCAACTCTGAGCCGCCTCTTAACCGTTTGATGCACCACGGGTTCATCACTCCGGTCCCACTCCACTACGTGCGTAACCACGGAGCTGTACCTAAGGCCAACTGGAGCGACTGGACTATCGAGGTCTGCGGCCTCGTCAAACGCCCCGCCAAGTTCACCATGGACCAACTCCTCAACGAGTTCCGCTTCCGGGAGTTTCCGGCCACGCTAGTCTGCGCTGGAAACCGCCGGAAGGAGCAGAACATGGTGAAAAAGAGCATCGGGTTCAACTGGGGAGCAGCGGGTGTGTCCACGTCAGTTTGGAGGGGGGTCCCACTACGTGACCTGTTGAAGCGATGTGGGATTATGAGCCGCAAGAAGGGGGCGCTGAATGTGTGCTTTGAAGGGGCTGAGGACCTGCCAGGTGGCGGTGGGTCCAAGTACGGGACCAGTATCAAGAAAGAGCTAGCCATGGATCCGGCTAGGGATATCATTTTAGCCTACATGCAAAACGGAGAGCAGTTGGTACCGGACCACGGGTTTCCCGTGAGGATGATAATACCGGGATTCATCGGGGGTAGAATGGTCAAGTGGCTCAAACGCATCATTGTTACAACCAAAGAATCAGAAAATTATTACCATTTCAAGGACAATAGAGTTTTGCCTTCCCATGTTGATGCTGAGCTGGCCAACGCCGAAGGTAAATAATCACTCTTTTAATATACTAAAAATATAAAATTAGGAAAATTTTTAAAAATAGGATAATAAGAGAAATTATTTATACAAAATAAAAAAATGGTAGATAGTTATGATAAACGGTGATGAAAGTCTATTAAGGTCTGAAGTCTGTCACTAATAGATGTTGATAAAATTTTAACCATGTATATCAACATTTTTTTTATTATTATTTTCTATAAATAGTTTGATTTTTTTTTGTGTGAAATTTTTTCTTAAAATTAAATGTTTAATTAATATATGCAGCATGGTGGTACAAGCCGGAGTATATAATCAACGAGTTGAACATAAACTCGGTCATAACCACGCCCTGCCACGAGGAGATTCTGCCTATCAACGCCTGGACCACTCAGAGGCCGTACACCTTGAGAGGGTATTCTTATTCCGGTGAGCCGCCGCTGTTTTTCTTTTTTTAATATTTGACCCTTCTAGGCTAGGAATATTATAATGAATTTTTAATTAAGGAATCTGTCAATATTTGGAATAACTTTATTTTAATAAAAGGCTTTGTTTTACTTTAGTGTTATTTAGGAAAGGTAGATGTAGATCCGGTGAATTTTATTTTAATGTGTAAGTTTGAAAAACGTGTAGGTGGTGGGAAGAAGGTGACACGTGTGGAGGTGACAATGGACGGTGGAGAGACATGGCAAGTATGCACGTTAGACCATCCGGAAAAGCCAAACAAATATGGAAAATACTGGTGCTGGTGTTTCTGGTCGTTGGAGGTGGAGGTCCTTGATTTGCTTGGCGCTAAGGAGATTGCAGTCCGGGCTTGGGATGAAACCTTAAACACCCAACCTGAGAAACTCATATGGAACCTCATGGGAATGATGAACAATTGTTGGTTCACAGTGAAAACCAACGTATGCAAACCCCACAAGGGAGAAATCGGAATCATCTTCGAACACCCAACAGTACCCGGCAACCAATCTGGAGGATGGATGGACAGAGAGAGACATCTAGAAATGTCCTCTGAATCCAACCAAACCCTAAAGAAGAGTGTCTCCAGTCCATTCATGAACACCGCTTCCAACACTTACTCCTTATCCGAAGTCAAAAAACACAACTCCCCTCAATCCGCCTGGATCATTGTCCACGGCCATGTCTACGACTGCACACGCTTCCTCAAAGACCACCCCGGCGGTTCCGACAGTATCTTAATCAACGCCGGCACTGACTGCACCGAAGAATTCGACGCCATCCATTCCGACAAAGCCAAGAAAATGCTCGAGGATTATCGAATCGGAGAGTTGATCACCACTGGTTACACCTCCGACTCCTCCTCCAACTCCCCTAACAACTCCACTCACGGCGCTTCCAACTTCTCACACTTAGCCCCAATCCGAGAGACTCCAATCGTGTCGAAACGCGTGGCGCTGGCCCCCAACGAGAAAATCCCGTGCAAGCTGATTTCCAAAACTTCAATCTCACACGACGTGCGTGTGTTCAGATTCGAACTGCCCGGTGGTCAGGATCAGGCACTCGGGCTGCCCGTGGGTAAGCACATTTTCGTATGTGCTAAAGTGGACGGGAAGCTATGCATGAGAGCCTACACGCCCTCTAGCCCCATAGATACAATGGGCTACTTCGAATTAGTGGTCAAAGTGTACTTCAAAAACGTGCACCCTAAGTTCCCCAACGGCGGGATCATGTCGCAGTATTTGGACAACTTGGAAGTGGGCTCGTCGATTGAGGTTAAGGGTCCATTAGGCCACATCGAGTACACAGGGCGCGGCAACTTTATGATTCATGGGAAACCCCGGTTCGCTCGGCGGCTTGCCATGCTGGCAGGTGGGACTGGAATAACCCCGATTTATCAAGTGGTGCAGGCGATTTTGAAGGATCCGGAAGACGAGACGGAGATGTATGTGGTGTATGCGAATCGAACGGAGGATGATATTTTGCTGAGGGAAGAATTGGACACGTGGGCGAAGAAGAACGAGAGATTGAAAGTATGGTACGTGGTGCAAGAGAGTATTAGAGAAGGATGGGAATATAGCATTGGGTTTATTACAGAGGATATTATGAGAGAGCATCTTCCGGCAGCGGCGGAGGATACTTTGGCTTTGGTTTGTGGGCCGCCGCCTATGATTCAGTTTGCGGTACAGCCCAATTTGGAGAAGATGAATTACGACACCAAGAATTCGATGTTGGTGTTTTGA

mRNA sequence

ATGGCGGCTTCCGTCGACAACCGCCAATTTGGATCTCTTCAGCCACCGCTTAACGGCGTCGTGCGCTCCTTCAAGTCCGGTTCTAACCACCGGTCCGACTCGCCGGTTCGTGGCTGTAACTTCGATTACAATAATAACAACCGCCTTCTCAAAAGTTCCGTGAAGATAGAACAGGAAGAGGAGGAGGATTCGTGTAGTGAAGACGAGAATGATAACTACGAGTTACGTGAGTTGATTCTGAAGGGAAAGCGTGAACTGGAGCCGTCGATTGTGGACCACAGAGATGAAGGGACGGCTGATAATTGGATCCAGCGGAACCCTTCCATGGTCCGTCTCACAGGGAAGCACCCCTTCAACTCTGAGCCGCCTCTTAACCGTTTGATGCACCACGGGTTCATCACTCCGGTCCCACTCCACTACGTGCGTAACCACGGAGCTGTACCTAAGGCCAACTGGAGCGACTGGACTATCGAGGTCTGCGGCCTCGTCAAACGCCCCGCCAAGTTCACCATGGACCAACTCCTCAACGAGTTCCGCTTCCGGGAGTTTCCGGCCACGCTAGTCTGCGCTGGAAACCGCCGGAAGGAGCAGAACATGGTGAAAAAGAGCATCGGGTTCAACTGGGGAGCAGCGGGTGTGTCCACGTCAGTTTGGAGGGGGGTCCCACTACGTGACCTGTTGAAGCGATGTGGGATTATGAGCCGCAAGAAGGGGGCGCTGAATGTGTGCTTTGAAGGGGCTGAGGACCTGCCAGGTGGCGGTGGGTCCAAGTACGGGACCAGTATCAAGAAAGAGCTAGCCATGGATCCGGCTAGGGATATCATTTTAGCCTACATGCAAAACGGAGAGCAGTTGGTACCGGACCACGGGTTTCCCGTGAGGATGATAATACCGGGATTCATCGGGGGTGGTGGGAAGAAGGTGACACGTGTGGAGGTGACAATGGACGGTGGAGAGACATGGCAAGTATGCACGTTAGACCATCCGGAAAAGCCAAACAAATATGGAAAATACTGGTGCTGGTGTTTCTGGTCGTTGGAGGTGGAGGTCCTTGATTTGCTTGGCGCTAAGGAGATTGCAGTCCGGGCTTGGGATGAAACCTTAAACACCCAACCTGAGAAACTCATATGGAACCTCATGGGAATGATGAACAATTGTTGGTTCACAGTGAAAACCAACGTATGCAAACCCCACAAGGGAGAAATCGGAATCATCTTCGAACACCCAACAGTACCCGGCAACCAATCTGGAGGATGGATGGACAGAGAGAGACATCTAGAAATGTCCTCTGAATCCAACCAAACCCTAAAGAAGAGTGTCTCCAGTCCATTCATGAACACCGCTTCCAACACTTACTCCTTATCCGAAGTCAAAAAACACAACTCCCCTCAATCCGCCTGGATCATTGTCCACGGCCATGTCTACGACTGCACACGCTTCCTCAAAGACCACCCCGGCGGTTCCGACAGTATCTTAATCAACGCCGGCACTGACTGCACCGAAGAATTCGACGCCATCCATTCCGACAAAGCCAAGAAAATGCTCGAGGATTATCGAATCGGAGAGTTGATCACCACTGGTTACACCTCCGACTCCTCCTCCAACTCCCCTAACAACTCCACTCACGGCGCTTCCAACTTCTCACACTTAGCCCCAATCCGAGAGACTCCAATCGTGTCGAAACGCGTGGCGCTGGCCCCCAACGAGAAAATCCCGTGCAAGCTGATTTCCAAAACTTCAATCTCACACGACGTGCGTGTGTTCAGATTCGAACTGCCCGGTGGTCAGGATCAGGCACTCGGGCTGCCCGTGGGTAAGCACATTTTCGTATGTGCTAAAGTGGACGGGAAGCTATGCATGAGAGCCTACACGCCCTCTAGCCCCATAGATACAATGGGCTACTTCGAATTAGTGGTCAAAGTGTACTTCAAAAACGTGCACCCTAAGTTCCCCAACGGCGGGATCATGTCGCAGTATTTGGACAACTTGGAAGTGGGCTCGTCGATTGAGGTTAAGGGTCCATTAGGCCACATCGAGTACACAGGGCGCGGCAACTTTATGATTCATGGGAAACCCCGGTTCGCTCGGCGGCTTGCCATGCTGGCAGGTGGGACTGGAATAACCCCGATTTATCAAGTGGTGCAGGCGATTTTGAAGGATCCGGAAGACGAGACGGAGATGTATGTGGTGTATGCGAATCGAACGGAGGATGATATTTTGCTGAGGGAAGAATTGGACACGTGGGCGAAGAAGAACGAGAGATTGAAAGTATGGTACGTGGTGCAAGAGAGTATTAGAGAAGGATGGGAATATAGCATTGGGTTTATTACAGAGGATATTATGAGAGAGCATCTTCCGGCAGCGGCGGAGGATACTTTGGCTTTGGTTTGTGGGCCGCCGCCTATGATTCAGTTTGCGGTACAGCCCAATTTGGAGAAGATGAATTACGACACCAAGAATTCGATGTTGGTGTTTTGA

Coding sequence (CDS)

ATGGCGGCTTCCGTCGACAACCGCCAATTTGGATCTCTTCAGCCACCGCTTAACGGCGTCGTGCGCTCCTTCAAGTCCGGTTCTAACCACCGGTCCGACTCGCCGGTTCGTGGCTGTAACTTCGATTACAATAATAACAACCGCCTTCTCAAAAGTTCCGTGAAGATAGAACAGGAAGAGGAGGAGGATTCGTGTAGTGAAGACGAGAATGATAACTACGAGTTACGTGAGTTGATTCTGAAGGGAAAGCGTGAACTGGAGCCGTCGATTGTGGACCACAGAGATGAAGGGACGGCTGATAATTGGATCCAGCGGAACCCTTCCATGGTCCGTCTCACAGGGAAGCACCCCTTCAACTCTGAGCCGCCTCTTAACCGTTTGATGCACCACGGGTTCATCACTCCGGTCCCACTCCACTACGTGCGTAACCACGGAGCTGTACCTAAGGCCAACTGGAGCGACTGGACTATCGAGGTCTGCGGCCTCGTCAAACGCCCCGCCAAGTTCACCATGGACCAACTCCTCAACGAGTTCCGCTTCCGGGAGTTTCCGGCCACGCTAGTCTGCGCTGGAAACCGCCGGAAGGAGCAGAACATGGTGAAAAAGAGCATCGGGTTCAACTGGGGAGCAGCGGGTGTGTCCACGTCAGTTTGGAGGGGGGTCCCACTACGTGACCTGTTGAAGCGATGTGGGATTATGAGCCGCAAGAAGGGGGCGCTGAATGTGTGCTTTGAAGGGGCTGAGGACCTGCCAGGTGGCGGTGGGTCCAAGTACGGGACCAGTATCAAGAAAGAGCTAGCCATGGATCCGGCTAGGGATATCATTTTAGCCTACATGCAAAACGGAGAGCAGTTGGTACCGGACCACGGGTTTCCCGTGAGGATGATAATACCGGGATTCATCGGGGGTGGTGGGAAGAAGGTGACACGTGTGGAGGTGACAATGGACGGTGGAGAGACATGGCAAGTATGCACGTTAGACCATCCGGAAAAGCCAAACAAATATGGAAAATACTGGTGCTGGTGTTTCTGGTCGTTGGAGGTGGAGGTCCTTGATTTGCTTGGCGCTAAGGAGATTGCAGTCCGGGCTTGGGATGAAACCTTAAACACCCAACCTGAGAAACTCATATGGAACCTCATGGGAATGATGAACAATTGTTGGTTCACAGTGAAAACCAACGTATGCAAACCCCACAAGGGAGAAATCGGAATCATCTTCGAACACCCAACAGTACCCGGCAACCAATCTGGAGGATGGATGGACAGAGAGAGACATCTAGAAATGTCCTCTGAATCCAACCAAACCCTAAAGAAGAGTGTCTCCAGTCCATTCATGAACACCGCTTCCAACACTTACTCCTTATCCGAAGTCAAAAAACACAACTCCCCTCAATCCGCCTGGATCATTGTCCACGGCCATGTCTACGACTGCACACGCTTCCTCAAAGACCACCCCGGCGGTTCCGACAGTATCTTAATCAACGCCGGCACTGACTGCACCGAAGAATTCGACGCCATCCATTCCGACAAAGCCAAGAAAATGCTCGAGGATTATCGAATCGGAGAGTTGATCACCACTGGTTACACCTCCGACTCCTCCTCCAACTCCCCTAACAACTCCACTCACGGCGCTTCCAACTTCTCACACTTAGCCCCAATCCGAGAGACTCCAATCGTGTCGAAACGCGTGGCGCTGGCCCCCAACGAGAAAATCCCGTGCAAGCTGATTTCCAAAACTTCAATCTCACACGACGTGCGTGTGTTCAGATTCGAACTGCCCGGTGGTCAGGATCAGGCACTCGGGCTGCCCGTGGGTAAGCACATTTTCGTATGTGCTAAAGTGGACGGGAAGCTATGCATGAGAGCCTACACGCCCTCTAGCCCCATAGATACAATGGGCTACTTCGAATTAGTGGTCAAAGTGTACTTCAAAAACGTGCACCCTAAGTTCCCCAACGGCGGGATCATGTCGCAGTATTTGGACAACTTGGAAGTGGGCTCGTCGATTGAGGTTAAGGGTCCATTAGGCCACATCGAGTACACAGGGCGCGGCAACTTTATGATTCATGGGAAACCCCGGTTCGCTCGGCGGCTTGCCATGCTGGCAGGTGGGACTGGAATAACCCCGATTTATCAAGTGGTGCAGGCGATTTTGAAGGATCCGGAAGACGAGACGGAGATGTATGTGGTGTATGCGAATCGAACGGAGGATGATATTTTGCTGAGGGAAGAATTGGACACGTGGGCGAAGAAGAACGAGAGATTGAAAGTATGGTACGTGGTGCAAGAGAGTATTAGAGAAGGATGGGAATATAGCATTGGGTTTATTACAGAGGATATTATGAGAGAGCATCTTCCGGCAGCGGCGGAGGATACTTTGGCTTTGGTTTGTGGGCCGCCGCCTATGATTCAGTTTGCGGTACAGCCCAATTTGGAGAAGATGAATTACGACACCAAGAATTCGATGTTGGTGTTTTGA

Protein sequence

MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNNNNRLLKSSVKIEQEEEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIPGFIGGGGKKVTRVEVTMDGGETWQVCTLDHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEDIMREHLPAAAEDTLALVCGPPPMIQFAVQPNLEKMNYDTKNSMLVF
Homology
BLAST of Lsi09G009870 vs. ExPASy Swiss-Prot
Match: P17569 (Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1545.8 bits (4001), Expect = 0.0e+00
Identity = 757/921 (82.19%), Postives = 795/921 (86.32%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNF-----DYNNNNRLLKSSVK 60
           MAASVDNRQ+G LQPPL+GVVRSFK+G NHR+DSPVRGCNF     DY NNNR LKSSVK
Sbjct: 1   MAASVDNRQYGPLQPPLSGVVRSFKNGPNHRADSPVRGCNFPNSNVDY-NNNRPLKSSVK 60

Query: 61  IEQ---EEEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRL 120
           I++   EE EDSCSEDEN+N E R+LI+KG RELEPSI+DHRDEGTADNWI+RN SMVRL
Sbjct: 61  IQEAAAEEMEDSCSEDENEN-EFRDLIVKGNRELEPSILDHRDEGTADNWIERNASMVRL 120

Query: 121 TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMD 180
           TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHG VPKA W+DWT+EVCGLVKRPAKFTMD
Sbjct: 121 TGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGVVPKAKWADWTVEVCGLVKRPAKFTMD 180

Query: 181 QLLNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGI 240
           QLLNEFRFREFPATLVCAGNRRKEQNMVK+SIGFNWGAAGVSTSVWR VPL DLLKRCGI
Sbjct: 181 QLLNEFRFREFPATLVCAGNRRKEQNMVKQSIGFNWGAAGVSTSVWRRVPLCDLLKRCGI 240

Query: 241 MSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFP 300
           +SRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFP
Sbjct: 241 LSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFP 300

Query: 301 VRMIIPGFIG-------------------------------------------------- 360
           VRMIIPGFIG                                                  
Sbjct: 301 VRMIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDADVANAEAWWYKPEHI 360

Query: 361 --------------------------------------GGGKKVTRVEVTMDGGETWQVC 420
                                                 GGGKKVTRVEVTMD GETWQVC
Sbjct: 361 INELNINSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTMDSGETWQVC 420

Query: 421 TLDHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMN 480
           TLDHPEK NKYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDET NTQPEKLIWNLMGMMN
Sbjct: 421 TLDHPEKANKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETHNTQPEKLIWNLMGMMN 480

Query: 481 NCWFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPF 540
           NCWF VKTN+CKPHKGEIGI+FEHPT PGNQSGGWMDRERHLE+S+ESNQTLKKSVS+PF
Sbjct: 481 NCWFRVKTNMCKPHKGEIGIVFEHPTQPGNQSGGWMDRERHLEISTESNQTLKKSVSTPF 540

Query: 541 MNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD 600
           MNTASNTY+LSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD
Sbjct: 541 MNTASNTYTLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD 600

Query: 601 AIHSDKAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVA 660
           AIHSDKAKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P VS+RVA
Sbjct: 601 AIHSDKAKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAP-VSRRVA 660

Query: 661 LAPNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYT 720
           LAPNEKIPCKLISKTSISHDVRVFRF LPGGQDQALGLPVGKHIF+CA VDGKLCMRAYT
Sbjct: 661 LAPNEKIPCKLISKTSISHDVRVFRFALPGGQDQALGLPVGKHIFICATVDGKLCMRAYT 720

Query: 721 PSSPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRG 780
           P+S ID MG+FELVVKVYFK VHPKFPNGGIMSQYLD++EVGS+++VKGPLGHIEYTGRG
Sbjct: 721 PTSSIDEMGFFELVVKVYFKGVHPKFPNGGIMSQYLDSMEVGSTLDVKGPLGHIEYTGRG 780

Query: 781 NFMIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREE 826
           NFM+HGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLR+E
Sbjct: 781 NFMVHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLRDE 840

BLAST of Lsi09G009870 vs. ExPASy Swiss-Prot
Match: P36859 (Nitrate reductase [NADH] OS=Petunia hybrida OX=4102 GN=NIA PE=2 SV=1)

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 637/917 (69.47%), Postives = 716/917 (78.08%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN--NNRLLKSSVKIEQ 60
           MAASV+NRQF  L+P L+GVVRSFK     RSDSPVRGCNF  NN   N   K +  I  
Sbjct: 1   MAASVENRQFSHLEPGLSGVVRSFKP----RSDSPVRGCNFPLNNELTNFQKKPNTTIYL 60

Query: 61  EEEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           +       +D++D  E  ++I KGK E+EPS+ D RDEGTADNWI+RN SM+RLTGKHPF
Sbjct: 61  DCSSSEDDDDDDDKNEYLQMIRKGKLEVEPSVHDIRDEGTADNWIERNNSMIRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPL RLMHHGFITPVPLHYVRNHG VPK  W DWT+EV GLVKRP KFTM+QL+NEF
Sbjct: 121 NSEPPLARLMHHGFITPVPLHYVRNHGPVPKGMWDDWTVEVTGLVKRPMKFTMEQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
             RE P TLVCAGNRRKEQNMVK++IGFNWGAA VST+VWRGVPLR +LKRCGI SR KG
Sbjct: 181 PSRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRAILKRCGIYSRTKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALN+CFEGA+ LPGGGGSKYGTSIKKE AMDP+RDII+AYMQNGE+L PDHGFP+RMIIP
Sbjct: 241 ALNICFEGADVLPGGGGSKYGTSIKKEFAMDPSRDIIIAYMQNGEKLTPDHGFPLRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTQESESYYHYKDNRVLPPHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETW VCT+DHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWNVCTVDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEKLIWN+MGMMNNCWF V
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFRV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGI+FEHPT PGN SGGWM +ERHLE+S+E+  TLKKS+S+PFMNTAS 
Sbjct: 481 KTNVCKPHKGEIGIVFEHPTQPGNLSGGWMAKERHLEISAEAPPTLKKSISTPFMNTASK 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
            YS+SEVKKHNS  SAWIIVHGHVYD TRFLKDHPGG DSILINAGTDCTEEFDAIHSDK
Sbjct: 541 MYSMSEVKKHNSADSAWIIVHGHVYDATRFLKDHPGGIDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFS-HLAPIRETPIVSKRVALAPNE 660
           AKK+LED+RIGELITTGYTSDS   SPNNS HG+S+FS  LAPI+E     + VAL P E
Sbjct: 601 AKKLLEDFRIGELITTGYTSDS---SPNNSVHGSSSFSGFLAPIKELAPAVRSVALIPRE 660

Query: 661 KIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPI 720
           KIPCKL+ K SISHDVR FRF LP  +DQ LGLPVGKHIF+CA +D KLCMRAYTP+S +
Sbjct: 661 KIPCKLVDKKSISHDVRKFRFALP-SEDQVLGLPVGKHIFLCAIIDDKLCMRAYTPTSTV 720

Query: 721 DTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIH 780
           D +GYFELVVK+YFK + PKFPNGG MSQYLD+L +G+ ++VKGPLGHIEY GRGNF++H
Sbjct: 721 DEVGYFELVVKIYFKGIVPKFPNGGQMSQYLDSLPLGAFVDVKGPLGHIEYQGRGNFLVH 780

Query: 781 GKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWA 826
           GK +FA++LAMLAGGTGITP+YQV+QAILKDPEDETEM+VVYANRTEDDILL++ELD+WA
Sbjct: 781 GKRKFAKKLAMLAGGTGITPVYQVMQAILKDPEDETEMHVVYANRTEDDILLKDELDSWA 840

BLAST of Lsi09G009870 vs. ExPASy Swiss-Prot
Match: P08509 (Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2)

HSP 1 Score: 1297.0 bits (3355), Expect = 0.0e+00
Identity = 635/918 (69.17%), Postives = 715/918 (77.89%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNNNNRLLK---SSVKIE 60
           MAASV+NRQF  L+    G+ RSFK     RSDSPVRGCNF   N+    K   S++ ++
Sbjct: 1   MAASVENRQFSHLEA---GLSRSFKP----RSDSPVRGCNFPSPNSTNFQKKPNSTIYLD 60

Query: 61  QEEEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHP 120
               ED   +DE + Y   ++I KG  ELEPS+ D RDEGTADNWI+RN SM+RLTGKHP
Sbjct: 61  YSSSEDDDDDDEKNEY--LQMIKKGNSELEPSVHDTRDEGTADNWIERNFSMIRLTGKHP 120

Query: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNE 180
           FNSEPPLNRLMHHGFITPVPLHYVRNHG VPK  W DWT+EV GLVKRP KFTMDQL+NE
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNE 180

Query: 181 FRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKK 240
           F  RE P TLVCAGNRRKEQNMVK++IGFNWGAA VST++WRGVPLR LLKRCG+ S+ K
Sbjct: 181 FPCRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTIWRGVPLRALLKRCGVFSKNK 240

Query: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMII 300
           GALNVCFEGA+ LPGGGGSKYGTSIKKE AMDPARDII+AYMQNGE+L PDHGFPVRMII
Sbjct: 241 GALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARDIIVAYMQNGEKLAPDHGFPVRMII 300

Query: 301 PGFIG------------------------------------------------------- 360
           PGFIG                                                       
Sbjct: 301 PGFIGGRMVKWIKRIIVTTQESDSYYHFKDNRVLPPHVDAELANTEAWWYKPEYIINELN 360

Query: 361 ---------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHP 420
                                            GGGKKVTRVEVT+DGGETWQV TLDHP
Sbjct: 361 INSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTLDGGETWQVSTLDHP 420

Query: 421 EKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFT 480
           EKP KYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEKLIWN+MGMMNNCWF 
Sbjct: 421 EKPTKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFR 480

Query: 481 VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTAS 540
           VK NVCKPHKGEIGI+FEHPT PGNQSGGWM +ERHLE+S+E+ QTLKKS+S+PFMNTAS
Sbjct: 481 VKMNVCKPHKGEIGIVFEHPTQPGNQSGGWMAKERHLEISAEAPQTLKKSISTPFMNTAS 540

Query: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600
             YS+SEV+KH+S  SAWIIVHGH+YD TRFLKDHPGG+DSILINAGTDCTEEFDAIHSD
Sbjct: 541 KMYSMSEVRKHSSADSAWIIVHGHIYDATRFLKDHPGGTDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNF-SHLAPIRETPIVSKRVALAPN 660
           KAKK+LED+RIGELITTGYTSD    SP NS HG+S+F S LAPI+E     + VAL P 
Sbjct: 601 KAKKLLEDFRIGELITTGYTSD----SPGNSVHGSSSFSSFLAPIKELVPAQRSVALIPR 660

Query: 661 EKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSP 720
           EKIPCKLI K SISHDVR FRF LP  +DQ LGLPVGKHIF+CA +D KLCMRAYTP+S 
Sbjct: 661 EKIPCKLIDKQSISHDVRKFRFALP-SEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTST 720

Query: 721 IDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMI 780
           ID +GYFELVVK+YFK +HPKFPNGG MSQYLD++ +GS ++VKGPLGHIEY G+GNF++
Sbjct: 721 IDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSMPLGSFLDVKGPLGHIEYQGKGNFLV 780

Query: 781 HGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTW 826
           HGK +FA++LAM+AGGTGITP+YQV+QAILKDPED+TEMYVVYANRTEDDILL+EELD+W
Sbjct: 781 HGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDTEMYVVYANRTEDDILLKEELDSW 840

BLAST of Lsi09G009870 vs. ExPASy Swiss-Prot
Match: P27783 (Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1)

HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 628/914 (68.71%), Postives = 716/914 (78.34%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSN-HRSDSPVRGCNFDYNNNNRLLKSSVKIEQE 60
           MAASV+NR+F   +P +NG+VR+FK   N HRSDSP  G         R + SS K +  
Sbjct: 1   MAASVENRRFTHHEPAVNGLVRTFKPVPNSHRSDSPDLG---------RQIPSSPKKQVA 60

Query: 61  EEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPFN 120
             EDS SEDEN+N + +ELI KG  ELEPSI+D RDE TADNW++RN +MVRLTGKHPFN
Sbjct: 61  TGEDSSSEDENEN-DYKELIQKGNGELEPSILDPRDEATADNWVERNATMVRLTGKHPFN 120

Query: 121 SEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEFR 180
           SE PL RLMHHGFITP PLHYVRNHG VPKA W DW++EVCGLVKRPA+FTMD+L+ EFR
Sbjct: 121 SEAPLTRLMHHGFITPAPLHYVRNHGPVPKARWEDWSVEVCGLVKRPARFTMDRLVTEFR 180

Query: 181 FREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKGA 240
            REFP TLVCAGNRRKEQNMVKK+IGFNWGAAGVSTSVWRGVPLRD+LKRCGI SR +GA
Sbjct: 181 SREFPVTLVCAGNRRKEQNMVKKTIGFNWGAAGVSTSVWRGVPLRDVLKRCGIFSRGRGA 240

Query: 241 LNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIPG 300
            NVCFEGAEDLPGGGGSKYGTS+K E+AMDPARDIIL YMQNGE+L PDHGFPVRMIIPG
Sbjct: 241 FNVCFEGAEDLPGGGGSKYGTSVKYEMAMDPARDIILGYMQNGERLSPDHGFPVRMIIPG 300

Query: 301 FIG--------------------------------------------------------- 360
           FIG                                                         
Sbjct: 301 FIGGRMVKWLKRIIVTTKESDNYYHYNDNRVLPSHVDADVAKAEAWWYKPEHIINELNIN 360

Query: 361 -------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPEK 420
                                          GGG+KVTRVE+TM+GGE W+VC LDHPEK
Sbjct: 361 SVITTPCHEEILPINSWTTQRPYTLRGYAYSGGGRKVTRVEITMNGGEKWRVCALDHPEK 420

Query: 421 PNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTVK 480
           PNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDE  NTQPEKLIWN+MGMMNNCWF VK
Sbjct: 421 PNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEAHNTQPEKLIWNVMGMMNNCWFRVK 480

Query: 481 TNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASNT 540
           TNVCK H GEIGI FEHPTVPGN+SGGWM RE++LE SS++NQ+LKKSVSSPFMNT+S  
Sbjct: 481 TNVCKAHMGEIGIAFEHPTVPGNESGGWMAREKNLETSSDANQSLKKSVSSPFMNTSSKM 540

Query: 541 YSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKA 600
           +S+SEVKKHNS +SAWIIVHGH+YDCT FLKDHPGG+DSILINAGTDCTEEFDAIHSDKA
Sbjct: 541 FSMSEVKKHNSAESAWIIVHGHIYDCTHFLKDHPGGADSILINAGTDCTEEFDAIHSDKA 600

Query: 601 KKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEKI 660
           KKMLEDYRIGELITTGY SD    SPN++ HGASN SHLAPI+E   + + VAL P  KI
Sbjct: 601 KKMLEDYRIGELITTGYVSD----SPNSTVHGASNTSHLAPIKEIAPL-RNVALIPGAKI 660

Query: 661 PCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPIDT 720
           P KL+ K S+SHDVR+FR  LP   DQ LGLPVGKH+F+CA +D KLCMRAYTP+S ID 
Sbjct: 661 PTKLVYKKSLSHDVRLFRLALP-SDDQVLGLPVGKHVFLCATIDDKLCMRAYTPTSTIDE 720

Query: 721 MGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHGK 780
           +GY +LVVK+YFKN +P+FPNGG+MSQ+LD+L +GS + VKGPLGH+EYTGRGNF++HG+
Sbjct: 721 VGYLDLVVKIYFKNSNPRFPNGGLMSQHLDSLPIGSVLHVKGPLGHVEYTGRGNFLVHGE 780

Query: 781 PRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAKK 826
           P+FA+RLAM+AGGTGITPIYQV+QAILKDPEDETEM+VVYANRTEDDILLREELD WAKK
Sbjct: 781 PKFAKRLAMVAGGTGITPIYQVIQAILKDPEDETEMFVVYANRTEDDILLREELDDWAKK 840

BLAST of Lsi09G009870 vs. ExPASy Swiss-Prot
Match: P11605 (Nitrate reductase [NADH] 1 OS=Nicotiana tabacum OX=4097 GN=NIA1 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 630/918 (68.63%), Postives = 714/918 (77.78%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNNNNRLLK---SSVKIE 60
           MAASV+NRQF  ++    G+ RSFK     RSDSPVRGCNF   N+    K   S++ ++
Sbjct: 1   MAASVENRQFSHIEA---GLSRSFKP----RSDSPVRGCNFPPPNSTNFQKKPNSTIFLD 60

Query: 61  QEEEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHP 120
               ED   +DE + Y   ++I KG  ELEPS+ D RDEGTADNWI+RN S++RLTGKHP
Sbjct: 61  YSSSEDDDDDDEKNEY--LQMIKKGNSELEPSVHDSRDEGTADNWIERNFSLIRLTGKHP 120

Query: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNE 180
           FNSEPPLNRLMHHGFITPVPLHYVRNHG VPK  W DWT+EV GLVKRP KFTMDQL+NE
Sbjct: 121 FNSEPPLNRLMHHGFITPVPLHYVRNHGPVPKGTWDDWTVEVTGLVKRPMKFTMDQLVNE 180

Query: 181 FRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKK 240
           F  RE P TLVCAGNRRKEQNMVK++IGFNWGAA VST+VWRGVPLR LLKR G+ S+ K
Sbjct: 181 FPSRELPVTLVCAGNRRKEQNMVKQTIGFNWGAAAVSTTVWRGVPLRALLKRYGVFSKNK 240

Query: 241 GALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMII 300
           GALNVCFEGA+ LPGGGGSKYGTSIKKE AMDPARDII+AYMQNGE+L PDHGFPVRMII
Sbjct: 241 GALNVCFEGADVLPGGGGSKYGTSIKKEFAMDPARDIIIAYMQNGEKLAPDHGFPVRMII 300

Query: 301 PGFIG------------------------------------------------------- 360
           PGFIG                                                       
Sbjct: 301 PGFIGGRMVKWIKRIIVTTQESDSYYHFKDNRVLPPHVDAELANTEAWWYKPEYIINELN 360

Query: 361 ---------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHP 420
                                            GGGKKVTRVEVT+DGGETWQVCTLDHP
Sbjct: 361 INSVITTPCHEEILPINAWTTQRPYTLRGYSYSGGGKKVTRVEVTLDGGETWQVCTLDHP 420

Query: 421 EKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFT 480
           EKP KYGKYWCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEKLIWN+MGMMNNCWF 
Sbjct: 421 EKPTKYGKYWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKLIWNVMGMMNNCWFR 480

Query: 481 VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTAS 540
           VK NVCKPHKGEIGI+FEHPT PGNQSGGWM +ERHLE+S+E+  TLKKS+S+PFMNTAS
Sbjct: 481 VKMNVCKPHKGEIGIVFEHPTQPGNQSGGWMAKERHLEISAEAPPTLKKSISTPFMNTAS 540

Query: 541 NTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600
             YS+SEV+KH+S  SAWIIVHGH+YD TRFLKDHPGGSDSILINAGTDCTEEFDAIHSD
Sbjct: 541 KMYSMSEVRKHSSADSAWIIVHGHIYDATRFLKDHPGGSDSILINAGTDCTEEFDAIHSD 600

Query: 601 KAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNF-SHLAPIRETPIVSKRVALAPN 660
           KAKK+LE++RIGEL+TTGYTSD    SP NS HG+S+F S LAPI+E     + VAL P 
Sbjct: 601 KAKKLLEEFRIGELLTTGYTSD----SPGNSVHGSSSFSSFLAPIKELVPAQRSVALIPR 660

Query: 661 EKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSP 720
           EKIPCKLI K SIS DVR FRF LP  +DQ LGLPVGKHIF+CA +D KLCMRAYTP+S 
Sbjct: 661 EKIPCKLIDKQSISPDVRKFRFALP-SEDQVLGLPVGKHIFLCAVIDDKLCMRAYTPTST 720

Query: 721 IDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMI 780
           ID +GYFELVVK+YFK +HPKFPNGG MSQYLD+L++GS ++VKGPLGHIEY G+GNF++
Sbjct: 721 IDEVGYFELVVKIYFKGIHPKFPNGGQMSQYLDSLQLGSFLDVKGPLGHIEYQGKGNFLV 780

Query: 781 HGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTW 826
           HGK +FA++LAM+AGGTGITP+YQV+QAILKDPED+TEMYVVYANRTEDDILL+EELD+W
Sbjct: 781 HGKQKFAKKLAMIAGGTGITPVYQVMQAILKDPEDDTEMYVVYANRTEDDILLKEELDSW 840

BLAST of Lsi09G009870 vs. ExPASy TrEMBL
Match: A0A5D3DS07 (Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G00730 PE=3 SV=1)

HSP 1 Score: 1601.3 bits (4145), Expect = 0.0e+00
Identity = 781/915 (85.36%), Postives = 807/915 (88.20%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG N RSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPSIVD RDE TADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRP KFTMDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRD+LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++RVALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPVVTRRVALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT+ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKV+GKLCMRAYTPS+ +D
Sbjct: 661 IPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRAYTPSTTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYFKNVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTG GNF +HG
Sbjct: 721 QMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGSGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. ExPASy TrEMBL
Match: A0A5A7USP6 (Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1991G00030 PE=3 SV=1)

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 780/915 (85.25%), Postives = 807/915 (88.20%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG N RSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPSIVD RDE TADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRP KFTMDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRD+LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++RVALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPVVTRRVALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT+ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKV+GKLCMRAYTPS+ +D
Sbjct: 661 IPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRAYTPSTTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYF+NVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTG GNF +HG
Sbjct: 721 QMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGSGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. ExPASy TrEMBL
Match: A0A1S3CCZ8 (Nitrate reductase OS=Cucumis melo OX=3656 GN=LOC103499266 PE=3 SV=1)

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 780/915 (85.25%), Postives = 807/915 (88.20%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG N RSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPSIVD RDE TADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRP KFTMDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRD+LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++RVALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPVVTRRVALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT+ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKV+GKLCMRAYTPS+ +D
Sbjct: 661 IPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRAYTPSTTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYF+NVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTG GNF +HG
Sbjct: 721 QMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGSGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. ExPASy TrEMBL
Match: E2IZV5 (Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR2 PE=2 SV=1)

HSP 1 Score: 1597.4 bits (4135), Expect = 0.0e+00
Identity = 778/915 (85.03%), Postives = 804/915 (87.87%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG NHRSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPS VD RDEGTADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDWTIEVCGLVKRP KF+MDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWTIEVCGLVKRPTKFSMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLR++LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++R ALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPMVTRRAALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKVDGKLCMRAYTPSS +D
Sbjct: 661 IPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSSTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYFKNVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTGRGNF +HG
Sbjct: 721 QMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQ+VQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. ExPASy TrEMBL
Match: E7BXW7 (Nitrate reductase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1590.9 bits (4118), Expect = 0.0e+00
Identity = 776/915 (84.81%), Postives = 803/915 (87.76%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG NHRSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPS VD RDEGTADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA W+DWTIEVCGLVKRP KF+MDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWADWTIEVCGLVKRPTKFSMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLR++LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGET QVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETRQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++R ALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPMVTRRAALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKVDGKLCMRAYTPSS +D
Sbjct: 661 IPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSSTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYFKNVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTGRGNF +HG
Sbjct: 721 QMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQ+VQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. NCBI nr
Match: XP_038907222.1 (nitrate reductase [NADH] [Benincasa hispida])

HSP 1 Score: 1606.3 bits (4158), Expect = 0.0e+00
Identity = 782/919 (85.09%), Postives = 807/919 (87.81%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNF-----DYNNNN-RLLKSSV 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG NHRSDSPVRGCNF     DYNNNN RLLKSSV
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNIDYNNNNGRLLKSSV 60

Query: 61  KIEQEEEEDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTG 120
            I+QEEEEDSCSEDENDNYE RELI KG RELEPSIVD RDEGTADNWI+RN SMVRLTG
Sbjct: 61  NIQQEEEEDSCSEDENDNYEFRELIQKGNRELEPSIVDPRDEGTADNWIERNSSMVRLTG 120

Query: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQL 180
           KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVP+A WSDWTIEVCGLVKRP KFTMDQL
Sbjct: 121 KHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPQAKWSDWTIEVCGLVKRPTKFTMDQL 180

Query: 181 LNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMS 240
           +NEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRD+LKRCGIMS
Sbjct: 181 VNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMS 240

Query: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300
           RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR
Sbjct: 241 RKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVR 300

Query: 301 MIIPGFIG---------------------------------------------------- 360
           MIIPGFIG                                                    
Sbjct: 301 MIIPGFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIIN 360

Query: 361 ------------------------------------GGGKKVTRVEVTMDGGETWQVCTL 420
                                               GGGKKVTRVEVTMDGGETWQVCTL
Sbjct: 361 ELNINSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTL 420

Query: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480
           DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC
Sbjct: 421 DHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNC 480

Query: 481 WFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMN 540
           WF VKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVS+PFMN
Sbjct: 481 WFRVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSTPFMN 540

Query: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600
           TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI
Sbjct: 541 TASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAI 600

Query: 601 HSDKAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALA 660
           HSDKAKKMLEDYRIGELITTGY SDSSS+SPNNSTHGASNFSHLAPI+E P++++RVAL 
Sbjct: 601 HSDKAKKMLEDYRIGELITTGYASDSSSSSPNNSTHGASNFSHLAPIKEAPMLTRRVALT 660

Query: 661 PNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPS 720
           PNEKIPCKLISKT+ISHDVRVFRFELPGG DQ+LGLPVGKHIF+CAKVDGKLCMRAYTPS
Sbjct: 661 PNEKIPCKLISKTAISHDVRVFRFELPGGLDQSLGLPVGKHIFICAKVDGKLCMRAYTPS 720

Query: 721 SPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNF 780
           +PID  GYFELVVKVYFKNVHPKFPNGG+MSQYLDN+EVGSS+E+KGPLGHIEYTGRGNF
Sbjct: 721 TPIDQTGYFELVVKVYFKNVHPKFPNGGLMSQYLDNMEVGSSVEIKGPLGHIEYTGRGNF 780

Query: 781 MIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELD 826
            +HGKPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTEDDILLREELD
Sbjct: 781 TVHGKPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELD 840

BLAST of Lsi09G009870 vs. NCBI nr
Match: TYK26501.1 (nitrate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 1601.3 bits (4145), Expect = 0.0e+00
Identity = 781/915 (85.36%), Postives = 807/915 (88.20%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG N RSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPSIVD RDE TADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRP KFTMDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRD+LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++RVALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPVVTRRVALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT+ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKV+GKLCMRAYTPS+ +D
Sbjct: 661 IPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRAYTPSTTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYFKNVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTG GNF +HG
Sbjct: 721 QMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGSGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. NCBI nr
Match: XP_008460453.1 (PREDICTED: nitrate reductase [NADH] [Cucumis melo] >KAA0057717.1 nitrate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 780/915 (85.25%), Postives = 807/915 (88.20%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG N RSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNRRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPSIVD RDE TADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSIVDPRDEATADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRP KFTMDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPTKFTMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRD+LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++RVALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPVVTRRVALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT+ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKV+GKLCMRAYTPS+ +D
Sbjct: 661 IPCKLISKTAISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVEGKLCMRAYTPSTTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYF+NVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTG GNF +HG
Sbjct: 721 QMGYFELVVKVYFRNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGSGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQVVQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQVVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. NCBI nr
Match: NP_001267696.1 (nitrate reductase [NADH]-like [Cucumis sativus] >ADN96689.1 nitrate reductase 2 [Cucumis sativus])

HSP 1 Score: 1597.4 bits (4135), Expect = 0.0e+00
Identity = 778/915 (85.03%), Postives = 804/915 (87.87%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG NHRSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPS VD RDEGTADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA WSDWTIEVCGLVKRP KF+MDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWSDWTIEVCGLVKRPTKFSMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLR++LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGETWQVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETWQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++R ALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPMVTRRAALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKVDGKLCMRAYTPSS +D
Sbjct: 661 IPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSSTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYFKNVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTGRGNF +HG
Sbjct: 721 QMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQ+VQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. NCBI nr
Match: ADK77877.1 (nitrate reductase [Cucumis sativus])

HSP 1 Score: 1590.9 bits (4118), Expect = 0.0e+00
Identity = 776/915 (84.81%), Postives = 803/915 (87.76%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNN-NNRLLKSSVKIEQE 60
           MAASVDNRQFGSLQPPLNGVVRSFKSG NHRSDSPVRGCNF  +N NNRLLKSSVK+EQE
Sbjct: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGPNHRSDSPVRGCNFPNSNINNRLLKSSVKMEQE 60

Query: 61  EE-EDSCSEDENDNYELRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGKHPF 120
           EE +DSCSEDENDN ELRELI KG RELEPS VD RDEGTADNWI+RN SMVRLTGKHPF
Sbjct: 61  EEDDDSCSEDENDNNELRELIKKGNRELEPSTVDPRDEGTADNWIERNSSMVRLTGKHPF 120

Query: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLLNEF 180
           NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA W+DWTIEVCGLVKRP KF+MDQL+NEF
Sbjct: 121 NSEPPLNRLMHHGFITPVPLHYVRNHGAVPKAKWADWTIEVCGLVKRPTKFSMDQLVNEF 180

Query: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSRKKG 240
           RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLR++LKRCGIMSRKKG
Sbjct: 181 RFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLREVLKRCGIMSRKKG 240

Query: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFPVRMIIP 300
           ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQL PDHGFPVRMIIP
Sbjct: 241 ALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYMQNGEQLAPDHGFPVRMIIP 300

Query: 301 GFIG-------------------------------------------------------- 360
           GFIG                                                        
Sbjct: 301 GFIGGRMVKWLKRIIVTTKESENYYHFKDNRVLPSHVDAELANAEAWWYKPEYIINELNI 360

Query: 361 --------------------------------GGGKKVTRVEVTMDGGETWQVCTLDHPE 420
                                           GGGKKVTRVEVTMDGGET QVCTLDHPE
Sbjct: 361 NSVITTPCHEEILPINSWTTQRPYTLRGYSYSGGGKKVTRVEVTMDGGETRQVCTLDHPE 420

Query: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480
           KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV
Sbjct: 421 KPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMNNCWFTV 480

Query: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPFMNTASN 540
           KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLE+S+ESNQTLKKS+S+PFMNTASN
Sbjct: 481 KTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEISTESNQTLKKSISTPFMNTASN 540

Query: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600
           TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK
Sbjct: 541 TYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFDAIHSDK 600

Query: 601 AKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSHLAPIRETPIVSKRVALAPNEK 660
           AKKMLEDYRIGELITTGY SDSSSNSPNNSTHGASNFSHLAPIRE P+V++R ALAPNEK
Sbjct: 601 AKKMLEDYRIGELITTGYASDSSSNSPNNSTHGASNFSHLAPIREAPMVTRRAALAPNEK 660

Query: 661 IPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAYTPSSPID 720
           IPCKLISKT ISHDVRVFRFELPGGQDQ LGLPVGKHIF+CAKVDGKLCMRAYTPSS +D
Sbjct: 661 IPCKLISKTEISHDVRVFRFELPGGQDQVLGLPVGKHIFICAKVDGKLCMRAYTPSSTVD 720

Query: 721 TMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGRGNFMIHG 780
            MGYFELVVKVYFKNVHPKFPNGGIMSQ+LDN+EVGS++EVKGPLGHIEYTGRGNF +HG
Sbjct: 721 QMGYFELVVKVYFKNVHPKFPNGGIMSQFLDNMEVGSTVEVKGPLGHIEYTGRGNFTVHG 780

Query: 781 KPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLREELDTWAK 826
           KPRFA+RLAMLAGGTGITPIYQ+VQAILKDPEDETEM+VVYANRTEDDILLREELDTWAK
Sbjct: 781 KPRFAKRLAMLAGGTGITPIYQIVQAILKDPEDETEMFVVYANRTEDDILLREELDTWAK 840

BLAST of Lsi09G009870 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 1250.0 bits (3233), Expect = 0.0e+00
Identity = 618/924 (66.88%), Postives = 703/924 (76.08%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFKSGSNHRSDSPVRGCNFDYNNNNRLLKSSVKIEQEE 60
           MAASVDNRQ+  L+P LNGVVRS+K     RSDSP    N   N    L  + V    ++
Sbjct: 1   MAASVDNRQYARLEPGLNGVVRSYKPPVPGRSDSPKAHQNQTTNQTVFLKPAKV---HDD 60

Query: 61  EEDSCSEDENDNYE-----LRELILKGKRELEPSIVDHRDEGTADNWIQRNPSMVRLTGK 120
           +ED  SEDEN+ +       +E+I K   ELEPS++D RDE TAD+WI+RNPSMVRLTGK
Sbjct: 61  DEDVSSEDENETHNSNAVYYKEMIRKSNAELEPSVLDPRDEYTADSWIERNPSMVRLTGK 120

Query: 121 HPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIEVCGLVKRPAKFTMDQLL 180
           HPFNSE PLNRLMHHGFITPVPLHYVRNHG VPKA W++WT+EV G VKRP KFTMDQL+
Sbjct: 121 HPFNSEAPLNRLMHHGFITPVPLHYVRNHGHVPKAQWAEWTVEVTGFVKRPMKFTMDQLV 180

Query: 181 NEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPLRDLLKRCGIMSR 240
           +EF +REF ATLVCAGNRRKEQNMVKKS GFNWG+AGVSTSVWRGVPL D+L+RCGI SR
Sbjct: 181 SEFAYREFAATLVCAGNRRKEQNMVKKSKGFNWGSAGVSTSVWRGVPLCDVLRRCGIFSR 240

Query: 241 KKGALNVCFEGAEDLPGG---GGSKYGTSIKKELAMDPARDIILAYMQNGEQLVPDHGFP 300
           K GALNVCFEG+EDLPGG    GSKYGTSIKKE AMDP+RDIILAYMQNGE L PDHGFP
Sbjct: 241 KGGALNVCFEGSEDLPGGAGTAGSKYGTSIKKEYAMDPSRDIILAYMQNGEYLTPDHGFP 300

Query: 301 VRMIIPGFIG-------------------------------------------------- 360
           VR+IIPGFIG                                                  
Sbjct: 301 VRIIIPGFIGGRMVKWLKRIIVTTKESDNFYHFKDNRVLPSLVDAELADEEGWWYKPEYI 360

Query: 361 --------------------------------------GGGKKVTRVEVTMDGGETWQVC 420
                                                 GGGKKVTRVEVT+DGGETW VC
Sbjct: 361 INELNINSVITTPCHEEILPINAFTTQRPYTLKGYAYSGGGKKVTRVEVTVDGGETWNVC 420

Query: 421 TLDHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNTQPEKLIWNLMGMMN 480
            LDH EKPNKYGK+WCWCFWSLEVEVLDLL AKEIAVRAWDETLNTQPEK+IWNLMGMMN
Sbjct: 421 ALDHQEKPNKYGKFWCWCFWSLEVEVLDLLSAKEIAVRAWDETLNTQPEKMIWNLMGMMN 480

Query: 481 NCWFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSSESNQTLKKSVSSPF 540
           NCWF VKTNVCKPHKGEIGI+FEHPT+PGN+SGGWM +ERHLE S+++  +LKKSVS+PF
Sbjct: 481 NCWFRVKTNVCKPHKGEIGIVFEHPTLPGNESGGWMAKERHLEKSADAPPSLKKSVSTPF 540

Query: 541 MNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDSILINAGTDCTEEFD 600
           MNT +  YS+SEVKKHNS  S WIIVHGH+YDCTRFL DHPGGSDSILINAGTDCTEEF+
Sbjct: 541 MNTTAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFE 600

Query: 601 AIHSDKAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASN-FSHLAPIRETPIVSKRV 660
           AIHSDKAKKMLEDYRIGELITTGY+SDSS  SPNNS HG+S  FS LAPI E   V    
Sbjct: 601 AIHSDKAKKMLEDYRIGELITTGYSSDSS--SPNNSVHGSSAVFSLLAPIGEATPVRNLA 660

Query: 661 ALAPNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKVDGKLCMRAY 720
            + P  K+P +L+ KTSISHDVR FRF LP  +D  LGLPVGKHIF+CA ++ KLC+RAY
Sbjct: 661 LVNPRAKVPVQLVEKTSISHDVRKFRFALP-VEDMVLGLPVGKHIFLCATINDKLCLRAY 720

Query: 721 TPSSPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIEYTGR 780
           TPSS +D +GYFELVVK+YF  VHP+FPNGG+MSQYLD+L +GS++E+KGPLGH+EY G+
Sbjct: 721 TPSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGK 780

Query: 781 GNFMIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDILLRE 826
           G+F +HGKP+FA +LAMLAGGTGITP+YQ++QAILKDPEDETEMYV+YANRTE+DILLRE
Sbjct: 781 GSFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLRE 840

BLAST of Lsi09G009870 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 605/940 (64.36%), Postives = 708/940 (75.32%), Query Frame = 0

Query: 1   MAASVDNRQFGSLQPPLNGVVRSFK--------SGSNHRSDSPVRGCNFDYNNNNRLLKS 60
           MA SVDNR +    P +NGV  +FK        S   HR            N    ++ +
Sbjct: 1   MATSVDNRHY----PTMNGVAHAFKPPLVPSPRSFDRHRHQ----------NQTLDVILT 60

Query: 61  SVKIEQEEE---------EDSCSEDENDNYE-----LRELILKGKRELEPSIVDHRDEGT 120
             KI +E E         +DS S+DE++++       +EL+ K   +LEPSI+D RDE T
Sbjct: 61  ETKIVKETEVITTVVDSYDDSSSDDEDESHNRNVPYYKELVKKSNSDLEPSILDPRDEST 120

Query: 121 ADNWIQRNPSMVRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKANWSDWTIE 180
           AD+WIQRN SM+RLTGKHPFN+E PL RLMHHGFITPVPLHYVRNHGAVPKANWSDW+IE
Sbjct: 121 ADSWIQRNSSMLRLTGKHPFNAEAPLPRLMHHGFITPVPLHYVRNHGAVPKANWSDWSIE 180

Query: 181 VCGLVKRPAKFTMDQLLNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVW 240
           + GLVKRPAKFTM++L++EF  REFP TLVCAGNRRKEQNMVK++IGFNWG+AGVSTS+W
Sbjct: 181 ITGLVKRPAKFTMEELISEFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLW 240

Query: 241 RGVPLRDLLKRCGIMSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAY 300
           +G+PL ++L+RCGI SR+ GALNVCFEGAEDLPGGGGSKYGTSIKKE+AMDPARDIILAY
Sbjct: 241 KGIPLSEILRRCGIYSRRGGALNVCFEGAEDLPGGGGSKYGTSIKKEMAMDPARDIILAY 300

Query: 301 MQNGEQLVPDHGFPVRMIIPGFIG------------------------------------ 360
           MQNGE L PDHGFPVR+I+PGFIG                                    
Sbjct: 301 MQNGELLTPDHGFPVRVIVPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPSLVDAE 360

Query: 361 ----------------------------------------------------GGGKKVTR 420
                                                               GGGKKVTR
Sbjct: 361 LANSEAWWYKPEYIINELNINSVITTPGHAEILPINAFTTQKPYTLKGYAYSGGGKKVTR 420

Query: 421 VEVTMDGGETWQVCTLDHPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDETLNT 480
           VEVT+DGG+TW VC LDH EKPNKYGK+WCWCFWSL+VEVLDLL AK++AVRAWDE+ NT
Sbjct: 421 VEVTLDGGDTWSVCELDHQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNT 480

Query: 481 QPEKLIWNLMGMMNNCWFTVKTNVCKPHKGEIGIIFEHPTVPGNQSGGWMDRERHLEMSS 540
           QP+KLIWNLMGMMNNCWF ++TNVCKPH+GEIGI+FEHPT PGNQSGGWM +ER LE+SS
Sbjct: 481 QPDKLIWNLMGMMNNCWFRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISS 540

Query: 541 ESNQTLKKSVSSPFMNTASNTYSLSEVKKHNSPQSAWIIVHGHVYDCTRFLKDHPGGSDS 600
           ESN TLKKSVSSPFMNTAS  YS+SEV+KHN+  SAWIIVHGH+YDCTRFLKDHPGG+DS
Sbjct: 541 ESNNTLKKSVSSPFMNTASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDS 600

Query: 601 ILINAGTDCTEEFDAIHSDKAKKMLEDYRIGELITTGYTSDSSSNSPNNSTHGASNFSH- 660
           ILINAGTDCTEEF+AIHSDKAKK+LEDYRIGELITTGY S     SPN S HGASNF   
Sbjct: 601 ILINAGTDCTEEFEAIHSDKAKKLLEDYRIGELITTGYDS-----SPNVSVHGASNFGPL 660

Query: 661 LAPIRE-TPIVSKRVALA-PNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKH 720
           LAPI+E TP   K +AL  P EKIP +LI KTSISHDVR FRF LP  +DQ LGLPVGKH
Sbjct: 661 LAPIKELTP--QKNIALVNPREKIPVRLIEKTSISHDVRKFRFALP-SEDQQLGLPVGKH 720

Query: 721 IFVCAKVDGKLCMRAYTPSSPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGS 780
           +FVCA ++ KLC+RAYTP+S ID +G+ +LVVKVYFK+VHP+FPNGG+MSQ+LD+L +GS
Sbjct: 721 VFVCANINDKLCLRAYTPTSAIDAVGHIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGS 780

Query: 781 SIEVKGPLGHIEYTGRGNFMIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEM 826
            I++KGPLGHIEY G+GNF++ GKP+FA++LAMLAGGTGITPIYQ++Q+IL DPEDETEM
Sbjct: 781 MIDIKGPLGHIEYKGKGNFLVSGKPKFAKKLAMLAGGTGITPIYQIIQSILSDPEDETEM 840

BLAST of Lsi09G009870 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 172.9 bits (437), Expect = 1.0e-42
Identity = 101/258 (39.15%), Postives = 153/258 (59.30%), Query Frame = 0

Query: 561 KRVALAPNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAK-VDGKLC 620
           +RV L P      KL+ +  +SH+V  F FELP      LGLP+G+HI    K   G+  
Sbjct: 38  RRVCLDPENFKEFKLVKRHQLSHNVAKFVFELP-TSTSVLGLPIGQHISCRGKDGQGEDV 97

Query: 621 MRAYTPSSPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPLGHIE 680
           ++ YTP++    +G FELV+K+Y     P+    G MS +   + VG  + VKGP G  +
Sbjct: 98  IKPYTPTTLDSDVGRFELVIKMY-----PQ----GRMSHHFREMRVGDHLAVKGPKGRFK 157

Query: 681 YTGRGNFMIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRTEDDI 740
           Y          +P   R   MLAGG+GITP++QV +AIL++P D+T+++++YAN T DDI
Sbjct: 158 Y----------QPGQFRAFGMLAGGSGITPMFQVARAILENPTDKTKVHLIYANVTYDDI 217

Query: 741 LLREELD-TWAKKNERLKVWYVVQESIREGWEYSIGFITEDIMREHLPAAAEDTLALVCG 800
           LL+EEL+       E+ K++YV+ +   E W+  +GF+++++++ H PA A D   L CG
Sbjct: 218 LLKEELEGLTTNYPEQFKIFYVLNQP-PEVWDGGVGFVSKEMIQTHCPAPASDIQILRCG 273

Query: 801 PPPMIQFAVQPNLEKMNY 817
           PPPM + A+  NLE + Y
Sbjct: 278 PPPMNK-AMAANLEALGY 273

BLAST of Lsi09G009870 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 160.2 bits (404), Expect = 7.0e-39
Identity = 89/249 (35.74%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 562 RVALAPNEKIPCKLISKTSISHDVRVFRFELPGGQDQALGLPVGKHIFVCAKV------D 621
           + AL P++ +  KL     +SH+ ++FRF      +  LGL V   +   A +       
Sbjct: 70  KTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAE--LGLHVASCLLTRAPLGYNAEGK 129

Query: 622 GKLCMRAYTPSSPIDTMGYFELVVKVYFKNVHPKFPNGGIMSQYLDNLEVGSSIEVKGPL 681
            K  +R YTP S  +  GYF+L++KVY        P+G  MSQ+  +L+ G  +EVKGP+
Sbjct: 130 TKYVIRPYTPISDPEAKGYFDLLIKVY--------PDGK-MSQHFASLKPGDVLEVKGPV 189

Query: 682 GHIEYTGRGNFMIHGKPRFARRLAMLAGGTGITPIYQVVQAILKDPEDETEMYVVYANRT 741
              +Y+          P   + + M+AGG+GITP+ QV+ AI+K+PED T++ ++YAN +
Sbjct: 190 EKFKYS----------PNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVS 249

Query: 742 EDDILLREELDTWAKKNERLKVWYVVQESIREGWEYSIGFITEDIMREHLPAAAEDTLAL 801
            DDILL+++LD     +  LK++Y V ++  + W+  +G+I++D+  + LP   +DTL L
Sbjct: 250 PDDILLKQKLDVLQANHPNLKIFYTV-DNPTKNWKGGVGYISKDMALKGLPLPTDDTLIL 296

Query: 802 VCGPPPMIQ 805
           VCGPP M++
Sbjct: 310 VCGPPGMME 296

BLAST of Lsi09G009870 vs. TAIR 10
Match: AT3G01910.1 (sulfite oxidase )

HSP 1 Score: 139.4 bits (350), Expect = 1.3e-32
Identity = 75/208 (36.06%), Postives = 113/208 (54.33%), Query Frame = 0

Query: 105 RNPSMVRLTGKHPFNSEPPLNRLMHHGFITPVPLHYVRNHGAVPKA-NWSDWTIEVCGLV 164
           R+PS+ ++  K PFN+EPP + L+   ++TPV L Y RNHG +P   +   +++ + GL+
Sbjct: 16  RHPSL-KVNAKEPFNAEPPRSALV-SSYVTPVDLFYKRNHGPIPIVDHLQSYSVTLTGLI 75

Query: 165 KRPAKFTMDQLLNEFRFREFPATLVCAGNRRKEQNMVKKSIGFNWGAAGVSTSVWRGVPL 224
           + P K  +  + +  ++    ATL CAGNRR   + V+   G  W  + +  +VW G  L
Sbjct: 76  QNPRKLFIKDIRSLPKY-NVTATLQCAGNRRTAMSKVRNVRGVGWDVSAIGNAVWGGAKL 135

Query: 225 RDLLKRCGI----MSRKKGALNVCFEGAEDLPGGGGSKYGTSIKKELAMDPARDIILAYM 284
            D+L+  GI     S   GA +V F   +      G  Y  SI    A +P  D++LAY 
Sbjct: 136 ADVLELVGIPKLTASTNLGARHVEFVSVDRCKEENGGPYKASITLSQATNPEADVLLAYE 195

Query: 285 QNGEQLVPDHGFPVRMIIPGFIGGGGKK 308
            NGE L  DHGFP+R+++PG IG    K
Sbjct: 196 MNGETLNRDHGFPLRVVVPGVIGARSVK 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P175690.0e+0082.19Nitrate reductase [NADH] OS=Cucurbita maxima OX=3661 PE=2 SV=1[more]
P368590.0e+0069.47Nitrate reductase [NADH] OS=Petunia hybrida OX=4102 GN=NIA PE=2 SV=1[more]
P085090.0e+0069.17Nitrate reductase [NADH] 2 OS=Nicotiana tabacum OX=4097 GN=NIA2 PE=2 SV=2[more]
P277830.0e+0068.71Nitrate reductase [NAD(P)H] OS=Betula pendula OX=3505 GN=NIA1 PE=2 SV=1[more]
P116050.0e+0068.63Nitrate reductase [NADH] 1 OS=Nicotiana tabacum OX=4097 GN=NIA1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DS070.0e+0085.36Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G00... [more]
A0A5A7USP60.0e+0085.25Nitrate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1991G0... [more]
A0A1S3CCZ80.0e+0085.25Nitrate reductase OS=Cucumis melo OX=3656 GN=LOC103499266 PE=3 SV=1[more]
E2IZV50.0e+0085.03Nitrate reductase OS=Cucumis sativus OX=3659 GN=NR2 PE=2 SV=1[more]
E7BXW70.0e+0084.81Nitrate reductase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038907222.10.0e+0085.09nitrate reductase [NADH] [Benincasa hispida][more]
TYK26501.10.0e+0085.36nitrate reductase [Cucumis melo var. makuwa][more]
XP_008460453.10.0e+0085.25PREDICTED: nitrate reductase [NADH] [Cucumis melo] >KAA0057717.1 nitrate reducta... [more]
NP_001267696.10.0e+0085.03nitrate reductase [NADH]-like [Cucumis sativus] >ADN96689.1 nitrate reductase 2 ... [more]
ADK77877.10.0e+0084.81nitrate reductase [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G37130.10.0e+0066.88nitrate reductase 2 [more]
AT1G77760.10.0e+0064.36nitrate reductase 1 [more]
AT5G17770.11.0e-4239.15NADH:cytochrome B5 reductase 1 [more]
AT5G20080.17.0e-3935.74FAD/NAD(P)-binding oxidoreductase [more]
AT3G01910.11.3e-3236.06sulfite oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 56..76
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 560..675
e-value: 9.0E-40
score: 137.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR19370:SF198NITRATE REDUCTASEcoord: 70..292
coord: 298..825
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 70..292
coord: 298..825
NoneNo IPR availableCDDcd06183cyt_b5_reduct_likecoord: 573..825
e-value: 6.846E-108
score: 328.372
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 484..498
score: 68.48
coord: 474..484
score: 70.66
coord: 511..523
score: 61.54
coord: 499..506
score: 64.2
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 452..524
e-value: 2.2E-29
score: 113.6
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 453..523
e-value: 5.8E-25
score: 87.2
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 449..524
score: 27.806641
IPR001709Flavoprotein pyridine nucleotide cytochrome reductasePRINTSPR00371FPNCRcoord: 725..734
score: 38.28
coord: 483..493
score: 38.56
coord: 793..801
score: 45.08
coord: 737..748
score: 47.32
coord: 699..718
score: 47.76
coord: 769..785
score: 27.72
coord: 621..628
score: 53.74
coord: 654..663
score: 27.24
IPR008335Eukaryotic molybdopterin oxidoreductasePRINTSPR00407EUMOPTERINcoord: 185..196
score: 91.11
coord: 196..208
score: 75.04
coord: 132..148
score: 80.26
coord: 110..124
score: 82.67
coord: 211..226
score: 64.31
coord: 269..285
score: 70.46
coord: 286..299
score: 76.83
IPR001834NADH:cytochrome b5 reductase-likePRINTSPR00406CYTB5RDTASEcoord: 600..611
score: 71.7
coord: 699..718
score: 72.6
coord: 793..801
score: 75.0
coord: 737..748
score: 72.22
coord: 621..628
score: 71.35
coord: 666..680
score: 63.75
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 446..526
e-value: 2.1E-29
score: 103.4
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 449..526
IPR036374Oxidoreductase, molybdopterin-binding domain superfamilyGENE3D3.90.420.10coord: 93..326
e-value: 2.6E-75
score: 254.7
IPR036374Oxidoreductase, molybdopterin-binding domain superfamilySUPERFAMILY56524Oxidoreductase molybdopterin-binding domaincoord: 92..313
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 700..806
e-value: 1.9E-34
score: 118.6
IPR012137Nitrate reductase NADH dependentPIRSFPIRSF000233Nitr_rd_NADHcoord: 27..304
e-value: 2.2E-116
score: 387.6
coord: 302..824
e-value: 2.2E-210
score: 698.6
IPR008333Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domainPFAMPF00970FAD_binding_6coord: 573..680
e-value: 4.4E-33
score: 113.6
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 676..825
e-value: 1.3E-47
score: 163.3
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 669..820
IPR000572Oxidoreductase, molybdopterin-binding domainPFAMPF00174Oxidored_molybcoord: 144..315
e-value: 3.5E-46
score: 156.9
IPR005066Moybdenum cofactor oxidoreductase, dimerisationPFAMPF03404Mo-co_dimercoord: 296..393
e-value: 6.2E-39
score: 133.2
IPR022407Oxidoreductase, molybdopterin binding sitePROSITEPS00559MOLYBDOPTERIN_EUKcoord: 161..196
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 480..487
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 568..681
score: 15.386329
IPR014756Immunoglobulin E-setSUPERFAMILY81296E set domainscoord: 302..394
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 563..681

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G009870.1Lsi09G009870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0006809 nitric oxide biosynthetic process
molecular_function GO:0071949 FAD binding
molecular_function GO:0020037 heme binding
molecular_function GO:0030151 molybdenum ion binding
molecular_function GO:0043546 molybdopterin cofactor binding
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0050464 nitrate reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity