Lsi09G008990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G008990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRibonuclease H
Locationchr09: 10737327 .. 10739111 (+)
RNA-Seq ExpressionLsi09G008990
SyntenyLsi09G008990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TACCACATTACACGTCATACAATCCTATGTATGAGTTACCAATGGGACGTTTCACTCATCCTAATGTGAAAACCTCGAGAACGAGTTAGTCTAGTGTCTTAATTCAAAAGCAAGAAGTATCCAATATACCTTTTATGATGAATAATTTAGATGACCCCGAGGTCCGAAAAAATCTGACAGATGAAGACAAGAAAACTCCTAGTAATGGAACATTTGAGGTCTTTGAAGAAAGGCTAAGGGCAATAGAAGGGACAAATGTTTTTCGTCATGTTGATGCCACCAAATTATGTCTGGTACCCGATGTTATTCTCCCACCTAAGTTCAAAGTTCCAAAATTCGAATATGATGGTTCGACCTGTTCAAAAAGCCATCTTATTATGTATTGCAGGAAGATGTCGGCTTATGCTCAAAACGACAAACTCCTCAAACACTATTTCCAGGACAGTTTAGCTAGACTAGCAGCTCATTGGTACATGCAACTCGAGGGTACACACATCTCGTCCTAGAGGAATTTAGCTGATTCTTTCCTAAAGCAGTATAAAAATAATATTGACATGGCATCGGATAGACTAGACTTCCAAAGAATGGAGAAGAAGAGTACAGGGGGTTTTAAAGAATACGCCCAAAGATGGAGAGATACAGCTGCCCAGGTTCAACCACCATTAACAGATAAAGAGTTGTCTGCAATGTTTATTAATACTCTAAAGTCGCCATTCTACAACCACATGATTAGGAGTGCTTCCACAAATTTCTCTGATATTATGACAATTGGAGAAAGGATTGAATATGGAATCAAACATGGTAGAATAGCCCCAGTCTCAGACAACAACACAGAAGAAGGGATCTTGGTTGAAAAAGAAGGAAGGAGAAGTACACATGGTAGGGAAGATTGAAAATTCCATACAACAACCTTATCAGCAAATGCCGCAATACTCTCCAAGTTTTTATCCACCCCCATATGGATATTATCCATATCAGGTGAATAATACATCTATGCAATATTCTCCTTATGTTGGTCAAGGAGTAAAACCTCAAACAAGTCAAGACTTTCAACCTTGTAAGCAACAACAGGTAAATGCTCCTAAGCAAGAAAGTAACCCGATGACAAATAGGCCACAGTTTGATCCAATGCCCATGACGTATACTGAACTTCTGCTTAAGTTAATCCAGAACCATCAATTGACTCCCAAGCCTATACGGCCGATTCAACCTCCCTATCCATGGTGGTATGACCCAAATGCCAGATGTGATTATCATGTGGGAGCTATCAGGCATTCGACAAAAAATTGTACTGCTTTAAAATACAAAGTTCAGGAGCTGGTTAAGTCAGGTTGATTAAACTTTGAAAAGGAAGAAGGGCCTAATATCAATAACAACTCTTTGCCAAATCACCAAGTAAATGTCGTAGAGTGTTTGGACATCAATTTGAAGACCAGGGTTGCATATATGAACATTCCTATGAAACAACTTTTTGATGTTCTCTTGGAAAACAAATATATTTCTATTGAACATTCGAGCTTAGTCCTTGATTGTGAGAAGCATGACAATAAGTTGACTTGCCTTTTCCATGCTGGGGTAAAAGGTCATTTGCTAGATGAATGTTCTCGTTTTCATGAAAAAGTTTAGGAGTTGATTGAAATGAAAGTTCTTACTATCACACAAGCTAGTCGCTCTTGTCAAGATCGTGAAATATGTATAGTGGAAGAGTCATCTTCCAAAGAAGATACTAATGTGACACTCAAACGAAAGTCTTTGACAATAACTTATCGAGAAAAACCTGATCCA

mRNA sequence

TACCACATTACACGTCATACAATCCTATGTATGAGTTACCAATGGGACGTTTCACTCATCCTAATGTGAAAACCTCGAGAACGAGGAAGATTGAAAATTCCATACAACAACCTTATCAGCAAATGCCGCAATACTCTCCAAGTTTTTATCCACCCCCATATGGATATTATCCATATCAGGTGAATAATACATCTATGCAATATTCTCCTTATGTTGGTCAAGGAACCAGGGTTGCATATATGAACATTCCTATGAAACAACTTTTTGATGTTCTCTTGGAAAACAAATATATTTCTATTGAACATTCGAGCTTAGTCCTTGATTGTGAGAAGCATGACAATAAGTTGACTTGCCTTTTCCATGCTGGGGTAAAAGGTCATTTGCTAGATGAATGTTCTCGTTTTCATGAAAAAGAGTTGATTGAAATGAAAGTTCTTACTATCACACAAGCTAGTCGCTCTTGTCAAGATCGTGAAATATGTATAGTGGAAGAGTCATCTTCCAAAGAAGATACTAATGTGACACTCAAACGAAAGTCTTTGACAATAACTTATCGAGAAAAACCTGATCCA

Coding sequence (CDS)

ATGTATGAGTTACCAATGGGACGTTTCACTCATCCTAATGTGAAAACCTCGAGAACGAGGAAGATTGAAAATTCCATACAACAACCTTATCAGCAAATGCCGCAATACTCTCCAAGTTTTTATCCACCCCCATATGGATATTATCCATATCAGGTGAATAATACATCTATGCAATATTCTCCTTATGTTGGTCAAGGAACCAGGGTTGCATATATGAACATTCCTATGAAACAACTTTTTGATGTTCTCTTGGAAAACAAATATATTTCTATTGAACATTCGAGCTTAGTCCTTGATTGTGAGAAGCATGACAATAAGTTGACTTGCCTTTTCCATGCTGGGGTAAAAGGTCATTTGCTAGATGAATGTTCTCGTTTTCATGAAAAAGAGTTGATTGAAATGAAAGTTCTTACTATCACACAAGCTAGTCGCTCTTGTCAAGATCGTGAAATATGTATAGTGGAAGAGTCATCTTCCAAAGAAGATACTAATGTGACACTCAAACGAAAGTCTTTGACAATAACTTATCGAGAAAAACCTGATCCA

Protein sequence

MYELPMGRFTHPNVKTSRTRKIENSIQQPYQQMPQYSPSFYPPPYGYYPYQVNNTSMQYSPYVGQGTRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLTCLFHAGVKGHLLDECSRFHEKELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVTLKRKSLTITYREKPDP
Homology
BLAST of Lsi09G008990 vs. ExPASy TrEMBL
Match: A0A6J1DM29 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia OX=3673 GN=LOC111022231 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.7e-17
Identity = 54/135 (40.00%), Postives = 88/135 (65.19%), Query Frame = 0

Query: 53  NNTSMQYSPYVGQG----TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLT 112
           N+ ++Q +    QG    ++V+ +  PM+ LF++L  + Y+S+EH    + CE++D  LT
Sbjct: 539 NHKNVQINAVECQGIESRSKVSEITTPMQXLFEILWXHGYMSMEHLCPDIRCERYDENLT 598

Query: 113 CLFHAGVKGHLLDECSRFHEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVT 172
           C +HAG +GH L++CS F EK  EL+++K+LT+TQ   S Q++ I +VE  S+ E ++  
Sbjct: 599 CPYHAGARGHPLEQCSCFKEKVQELLDLKILTVTQ---SHQEQRIDVVEYVSTAESSSAA 658

Query: 173 LKRKSLTITYREKPD 182
            K K LT+ YREKP+
Sbjct: 659 YKPKPLTVLYREKPE 670

BLAST of Lsi09G008990 vs. ExPASy TrEMBL
Match: A0A6J1DZ90 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.3e-14
Identity = 53/135 (39.26%), Postives = 84/135 (62.22%), Query Frame = 0

Query: 53  NNTSMQYSPYVGQG----TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLT 112
           N+ ++Q +    QG    ++VA +  P ++LF++LL + Y+S+E+    L  +++D  LT
Sbjct: 430 NHQNVQINAIECQGIESKSKVADIRTPKEELFEILLGSGYVSVEYLCPNLKYKEYDESLT 489

Query: 113 CLFHAGVKGHLLDECSRFHEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVT 172
           C FHAG KGH L++C+ F  K  EL++ K+LT+   + S Q + I +VE+ S  E ++  
Sbjct: 490 CPFHAGAKGHSLEQCNSFRMKVQELLDSKILTV---ANSHQKKGINVVEDVSVAEGSSDA 549

Query: 173 LKRKSLTITYREKPD 182
           LK K LTI Y EKPD
Sbjct: 550 LKPKRLTIFYSEKPD 561

BLAST of Lsi09G008990 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.1e-13
Identity = 50/117 (42.74%), Postives = 75/117 (64.10%), Query Frame = 0

Query: 67  TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLTCLFHAGVKGHLLDECSRF 126
           ++VA +  PM +LF++LL + Y+S+E+    L  + +D  LTC FHAG KGH L++C+ F
Sbjct: 455 SKVADIRTPMVELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFHAGAKGHSLEQCNSF 514

Query: 127 HEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVTLKRKSLTITYREKPD 182
             K  EL++ K+LT+   + S Q + I IVE+ S  E ++  LK K LTI Y EKP+
Sbjct: 515 RMKVQELLDSKILTV---ANSHQKKGINIVEDVSVAEGSSDALKPKCLTIFYSEKPN 568

BLAST of Lsi09G008990 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-01
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 0

Query: 27  QQPYQQMPQYSPSFYPPPYGYYPYQVNNTSMQYSPYVGQGTR 69
           QQPY + P+Y+P +YP PYGY    VNN +  YSPY  Q  R
Sbjct: 264 QQPYSRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYTFQNFR 305


HSP 2 Score: 84.0 bits (206), Expect = 7.3e-13
Identity = 50/122 (40.98%), Postives = 76/122 (62.30%), Query Frame = 0

Query: 67  TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLTCLFHAGVKGHLLDECSRF 126
           ++VA +  PM++LF++LL + Y+S+E+    L  + +D  LTC FHAG KGH L++C+ F
Sbjct: 390 SKVADITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFHAGAKGHALEQCNSF 449

Query: 127 H--EKELIEMKVLTITQASRSCQDREICIVE-----ESSSKEDTNVTLKRKSLTITYREK 182
               +EL++ K+LT+   + S Q + I +VE     E S  E ++  LK K LTI Y EK
Sbjct: 450 RMIVQELLDSKILTV---ANSHQKKGINVVEDVSVAEGSIAEGSSDALKPKRLTIFYSEK 508

BLAST of Lsi09G008990 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.1e-11
Identity = 52/135 (38.52%), Postives = 80/135 (59.26%), Query Frame = 0

Query: 53  NNTSMQYSPYVGQG----TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLT 112
           N+ ++Q +    QG    ++VA +  PM +LF++LL + YIS+E+  L    + +D  LT
Sbjct: 477 NHQNVQINAIECQGIESKSKVADITTPMXELFEILLGSGYISVEY--LCPKYKGYDESLT 536

Query: 113 CLFHAGVKGHLLDECSRFHEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVT 172
           C FH G KGH L++C+ F  K  EL++ K+LT    + S   +   +VE+    E ++ +
Sbjct: 537 CXFHXGAKGHSLEQCNXFRMKVQELLDSKILT---XANSHXKKXTNVVEDILVAEGSSDS 596

Query: 173 LKRKSLTITYREKPD 182
           LK K LTI YREKPD
Sbjct: 597 LKPKPLTIFYREKPD 606

BLAST of Lsi09G008990 vs. NCBI nr
Match: XP_022155098.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica charantia])

HSP 1 Score: 99.4 bits (246), Expect = 3.5e-17
Identity = 54/135 (40.00%), Postives = 88/135 (65.19%), Query Frame = 0

Query: 53  NNTSMQYSPYVGQG----TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLT 112
           N+ ++Q +    QG    ++V+ +  PM+ LF++L  + Y+S+EH    + CE++D  LT
Sbjct: 539 NHKNVQINAVECQGIESRSKVSEITTPMQXLFEILWXHGYMSMEHLCPDIRCERYDENLT 598

Query: 113 CLFHAGVKGHLLDECSRFHEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVT 172
           C +HAG +GH L++CS F EK  EL+++K+LT+TQ   S Q++ I +VE  S+ E ++  
Sbjct: 599 CPYHAGARGHPLEQCSCFKEKVQELLDLKILTVTQ---SHQEQRIDVVEYVSTAESSSAA 658

Query: 173 LKRKSLTITYREKPD 182
            K K LT+ YREKP+
Sbjct: 659 YKPKPLTVLYREKPE 670

BLAST of Lsi09G008990 vs. NCBI nr
Match: XP_022157796.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia])

HSP 1 Score: 89.0 bits (219), Expect = 4.7e-14
Identity = 53/135 (39.26%), Postives = 84/135 (62.22%), Query Frame = 0

Query: 53  NNTSMQYSPYVGQG----TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLT 112
           N+ ++Q +    QG    ++VA +  P ++LF++LL + Y+S+E+    L  +++D  LT
Sbjct: 430 NHQNVQINAIECQGIESKSKVADIRTPKEELFEILLGSGYVSVEYLCPNLKYKEYDESLT 489

Query: 113 CLFHAGVKGHLLDECSRFHEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVT 172
           C FHAG KGH L++C+ F  K  EL++ K+LT+   + S Q + I +VE+ S  E ++  
Sbjct: 490 CPFHAGAKGHSLEQCNSFRMKVQELLDSKILTV---ANSHQKKGINVVEDVSVAEGSSDA 549

Query: 173 LKRKSLTITYREKPD 182
           LK K LTI Y EKPD
Sbjct: 550 LKPKRLTIFYSEKPD 561

BLAST of Lsi09G008990 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 86.7 bits (213), Expect = 2.3e-13
Identity = 50/117 (42.74%), Postives = 75/117 (64.10%), Query Frame = 0

Query: 67  TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLTCLFHAGVKGHLLDECSRF 126
           ++VA +  PM +LF++LL + Y+S+E+    L  + +D  LTC FHAG KGH L++C+ F
Sbjct: 455 SKVADIRTPMVELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFHAGAKGHSLEQCNSF 514

Query: 127 HEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVTLKRKSLTITYREKPD 182
             K  EL++ K+LT+   + S Q + I IVE+ S  E ++  LK K LTI Y EKP+
Sbjct: 515 RMKVQELLDSKILTV---ANSHQKKGINIVEDVSVAEGSSDALKPKCLTIFYSEKPN 568

BLAST of Lsi09G008990 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 46.6 bits (109), Expect = 2.7e-01
Identity = 22/42 (52.38%), Postives = 27/42 (64.29%), Query Frame = 0

Query: 27  QQPYQQMPQYSPSFYPPPYGYYPYQVNNTSMQYSPYVGQGTR 69
           QQPY + P+Y+P +YP PYGY    VNN +  YSPY  Q  R
Sbjct: 264 QQPYSRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYTFQNFR 305


HSP 2 Score: 84.0 bits (206), Expect = 1.5e-12
Identity = 50/122 (40.98%), Postives = 76/122 (62.30%), Query Frame = 0

Query: 67  TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLTCLFHAGVKGHLLDECSRF 126
           ++VA +  PM++LF++LL + Y+S+E+    L  + +D  LTC FHAG KGH L++C+ F
Sbjct: 390 SKVADITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFHAGAKGHALEQCNSF 449

Query: 127 H--EKELIEMKVLTITQASRSCQDREICIVE-----ESSSKEDTNVTLKRKSLTITYREK 182
               +EL++ K+LT+   + S Q + I +VE     E S  E ++  LK K LTI Y EK
Sbjct: 450 RMIVQELLDSKILTV---ANSHQKKGINVVEDVSVAEGSIAEGSSDALKPKRLTIFYSEK 508

BLAST of Lsi09G008990 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 80.1 bits (196), Expect = 2.2e-11
Identity = 52/135 (38.52%), Postives = 80/135 (59.26%), Query Frame = 0

Query: 53  NNTSMQYSPYVGQG----TRVAYMNIPMKQLFDVLLENKYISIEHSSLVLDCEKHDNKLT 112
           N+ ++Q +    QG    ++VA +  PM +LF++LL + YIS+E+  L    + +D  LT
Sbjct: 477 NHQNVQINAIECQGIESKSKVABITTPMXELFEILLGSGYISVEY--LCPKYKGYDESLT 536

Query: 113 CLFHAGVKGHLLDECSRFHEK--ELIEMKVLTITQASRSCQDREICIVEESSSKEDTNVT 172
           C FH G KGH L++C+ F  K  EL++ K+LT    + S   +   +VE+    E ++ +
Sbjct: 537 CXFHXGAKGHSLEQCNXFRMKVQELLDSKILT---XANSHXKKXTNVVEDILVAEGSSDS 596

Query: 173 LKRKSLTITYREKPD 182
           LK K LTI YREKPD
Sbjct: 597 LKPKPLTIFYREKPD 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DM291.7e-1740.00LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia... [more]
A0A6J1DZ902.3e-1439.26Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1[more]
A0A6J1E2J71.1e-1342.74Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1[more]
A0A6J1E2J71.3e-0152.38Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1[more]
A0A6J1D0991.1e-1138.52Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1[more]

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Match NameE-valueIdentityDescription
XP_022155098.13.5e-1740.00LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica ch... [more]
XP_022157796.14.7e-1439.26LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia][more]
XP_022158986.12.3e-1342.74LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia][more]
XP_022158986.12.7e-0152.38LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia][more]
XP_022147189.12.2e-1138.52LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia][more]

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Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G008990.1Lsi09G008990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
molecular_function GO:0140640 catalytic activity, acting on a nucleic acid