Lsi09G008530 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G008530
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	calumenin-like
Location	chr09: 9829800 .. 9832713 (+)
RNA-Seq Expression	Lsi09G008530
Synteny	Lsi09G008530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTGTTGCCATCAGTCCCCTCAGTCTCAGCTGGTCAAGGAGAAAATGGGTGTGGCTGGAAGAGGATGGTAACATGAGAGGAGCGGGAGATGAAGAATTGGATTGTACTAATGGCTTTTGTCGATTGTTTTGCTTTTTTTTCCGTTTATTTTTGGTTTCCCCTCGTGTTAAGAGAAGATAGACAAAGCAGACAGTGGAGAAACATGATTTCTAGATTTCCATGCTTAAAAGCATATCAATTGGGAGGCATGCGAGCATAAAGGGAGCATGTTTGCTCTATATCAACGTCCACAAGAAAAAAATTACTGAAGAGTATTTTTTTTTCTTCTTTGAACCGACTTGGTATCTCTTCTAGGGATATCTGTGAGTTCTGGGAAGTTGAGAATTGACTTTGTAGTAAATTTGAAGAAGAATTTGAGTCGTATAGTCTGGTATACTGTCTATCACTGGATCTAGAATCTTTCACCACTTGATGTGGATATCCCATTCAACAAATTCATTGTAATTGAGCCTTCCCCAAACTTGAAATCTTAGTCATTGGCTTGATATCAAGCATACAAAGTTTTGGAAACAAAGTGCTGGGTGACTGGCTAAAATATTCAATCTTGAGAAGTGACGAAAGGCCAAGATCCCTCCTAGCAGTTCTCTGAAAACGTTTGTGGTAATCTTGAGTCCACAGTGAAAGAAGCATTTTTAGTCAAATATTGTTAGAGTACATATACACATATGGCAATGTTACATGTCACTTACTACATAAAAATAATAAATATATAATGCATGTAAATAAATGTAATTGCCTTATAACACGTGACAGGTATTCTTGTTATTGTAAATAAATCTATTACCATTCTCAAGAAATTTTTGTTGTCGTATTTGCACTCCAAAATCTATAGTTCCCGTTCATTTCACCATTTTATTGCTTCAATGGAATGATTCTTATAAATAATCTGTAAAGCAAAAAACGAAGGAGCTTTTACAGGTATATGTATAGCATCTACTTGTGCTTATCTGAGAACCAGAATTAGTGTATTTGGAAGGGTGTTACTTTTTTTTTTTTTTTTAATAATAATAATATTATTATTATTTTACTTTTGGGGTTTGGCATTTCATCAGCTTTGCTAACTAGTCAATTGGTGAGTGGCCTTGTAGGGAGAGAGATTCAGACAAGGATGGAAAGATTAACTTCAATGAGTTTTTCCATGGACTCTTTGACATGGTGAGAAATTATGATGAGAATCACAATTCTTCACATCATTCTGAAGATTCCAGGGATGGCCCTGCTAGAAACTTGTTTGCGGTGCTAGACAAAGATAATGACGGGTAACCAAAATTTATGATGTGGACAACTTCTTATTTGTTAACTGCCGAATTCCTTGGCAACTTTAATGATCTGTTTTTGTGTTATATTTTGTTTTTCATAGTCACCTGTCTGATGAAGAGCTGTTACCTATAATTGAAAAAATCCACCCATCAGAGCATTACTATGCAAAACAACAAGCAGAATATATCATACAGCAGGTACTCATGCTTGACGGAATCTTAAAACCCATATGAAAATTGATGAATTTTTTAGTTTTACGATTGAGATATTACTTGGATATGGCACGTATTAATGACATCAACAAACTTCTCATAATTCTTATGTATGGTTGTAGAAACATTTTACACTTTCTTTTTCCCTATCAATGAGGGCTTGCTGGGTTATAAATGGATTATTTTATACTCGTGTACTGTTATTTTAATTTTTACTAGACCAACCTCCCATCATATATAAGATATTGTGTAGAAATCATTTTGGAACTAGTAAAATCGGTCATATTAAAACACAACCACTACCAATTGTTTCCTGTTTTCACTTTGTGGGCTGAATTGCAATCTTTATCATTCTATTTGGAAGTTCCTTTATCAAATGGTAGATTCACTTGGTCTACTTTGGGGGATGTTTTCTTCAGATCGTTAATTGATCATTTTCTGATTTCATTATGAATGCATTGCATCCGACTTTCAACTCACTGAACTATAGCATAATTGTTAATGTTATTTTGTTCCTCGTTGATTTTAATCAGATAGACCAAGTTGTTTTCTCAGCCTCAGAACATTAAGATATAGTATGACATCATTTTATCTTATCATTTTACGTTGAAGGACTTTCTTGCAAATGTTTGTCGTATCGATGAGAAAACATTACCATAAGATCTAGACTAGACCTGGAAAATATTTTCTTCTTCCCGGGGGAGCAAGTATATCTGTTGATCAACAACTTCTTTGTGTCTTTATTTTGCAGGCTGATGCAGATAAAGATGGTCGTCTCACCTTGACAGAAATGATCGATCATCCTTACGTATTTTACAGTGCCATTTTCAATGAAGACGACGAGGATGATTATGATTTCCACGATGAGTTCCGTTAAGTTCTTTCAAGTATGGACACCTCCCTCTATCTATCAAACTTCTGCAGACAAGACCAGCATTGATGTGCTCTTGATAGTTTGTTGCTGTTGCAGGAATAGTTGAATAGTCATAGGTTAGCGGTAGAAGAAAACGGAGTTTTTTCGCAACATGGAATCAGGATACAGAGTTTGAAGTCTTAGGCAACAACTTGGAAGAGAGCTCTGGCATGGTGGTCAGAACTGGTTTAATCTTCTCCAGCCATGGCTTGCCCATATGGTATCCTCTTTTTGTAGCATATGAAACCCTTTATCTTGTCTAAGATAACCATGACTTCACACCTTAGATCATAGACATTTATTTAATTTGGTTGCAAATATTTGTTAAGTTCAGTGTCCTTGGTATAGAATTATCATTTAATTGTTATTTGTTACTAATTTATTCTTCATGGATAGAGAATTATCAATTATTCAAGGTTATATTTATCTTTTGAAGTATAGGGTTTTGAAAAGTGGGTAGAACAG

mRNA sequence

ATGAACTGTTGCCATCAGTCCCCTCAGTCTCAGCTGGTCAAGGAGAAAATGGGTGTGGCTGGAAGAGGATGGGAGAGAGATTCAGACAAGGATGGAAAGATTAACTTCAATGAGTTTTTCCATGGACTCTTTGACATGGTGAGAAATTATGATGAGAATCACAATTCTTCACATCATTCTGAAGATTCCAGGGATGGCCCTGCTAGAAACTTGTTTGCGGTGCTAGACAAAGATAATGACGGTCACCTGTCTGATGAAGAGCTGTTACCTATAATTGAAAAAATCCACCCATCAGAGCATTACTATGCAAAACAACAAGCAGAATATATCATACAGCAGGCTGATGCAGATAAAGATGGTCGTCTCACCTTGACAGAAATGATCGATCATCCTTACGTATTTTACAGTGCCATTTTCAATGAAGACGACGAGGATGATTATGATTTCCACGATGAGTTCCGTTAAGTTCTTTCAATTTGTTGCTGTTGCAGGAATAGTTGAATAGTCATAGGTTAGCGGTAGAAGAAAACGGAGTTTTTTCGCAACATGGAATCAGGATACAGAGTTTGAAGTCTTAGGCAACAACTTGGAAGAGAGCTCTGGCATGGTGGTCAGAACTGGTTTAATCTTCTCCAGCCATGGCTTGCCCATATGGTATCCTCTTTTTGTAGCATATGAAACCCTTTATCTTGTCTAAGATAACCATGACTTCACACCTTAGATCATAGACATTTATTTAATTTGGTTGCAAATATTTGTTAAGTTCAGTGTCCTTGGTATAGAATTATCATTTAATTGTTATTTGTTACTAATTTATTCTTCATGGATAGAGAATTATCAATTATTCAAGGTTATATTTATCTTTTGAAGTATAGGGTTTTGAAAAGTGGGTAGAACAG

Coding sequence (CDS)

ATGAACTGTTGCCATCAGTCCCCTCAGTCTCAGCTGGTCAAGGAGAAAATGGGTGTGGCTGGAAGAGGATGGGAGAGAGATTCAGACAAGGATGGAAAGATTAACTTCAATGAGTTTTTCCATGGACTCTTTGACATGGTGAGAAATTATGATGAGAATCACAATTCTTCACATCATTCTGAAGATTCCAGGGATGGCCCTGCTAGAAACTTGTTTGCGGTGCTAGACAAAGATAATGACGGTCACCTGTCTGATGAAGAGCTGTTACCTATAATTGAAAAAATCCACCCATCAGAGCATTACTATGCAAAACAACAAGCAGAATATATCATACAGCAGGCTGATGCAGATAAAGATGGTCGTCTCACCTTGACAGAAATGATCGATCATCCTTACGTATTTTACAGTGCCATTTTCAATGAAGACGACGAGGATGATTATGATTTCCACGATGAGTTCCGTTAA

Protein sequence

MNCCHQSPQSQLVKEKMGVAGRGWERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDEDDYDFHDEFR

Homology

[Close]

BLAST of Lsi09G008530 vs. ExPASy Swiss-Prot
Match: Q9JM83 (Calmodulin-4 OS=Mus musculus OX=10090 GN=Calm4 PE=2 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 2.0e-05
Identity = 32/102 (31.37%), Postives = 55/102 (53.92%), Query Frame = 0


Query: 27  DSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDE 86
           D+D DGKI+F EF       +  Y + H         R G  R +F VLD++ DG+++ +
Sbjct: 57  DTDGDGKISFEEF----LTAIEKYKKGH---------RAGELRAVFNVLDQNGDGYITVD 116

Query: 87  ELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMI 129
           EL   + K+  S    ++++ E +I+ AD D+DG++   E +
Sbjct: 117 ELKESLSKLGES---LSQEELEDMIRVADVDQDGKVKYEEFV 142

[Close]

BLAST of Lsi09G008530 vs. ExPASy Swiss-Prot
Match: Q91614 (Neuronal calcium sensor 1 OS=Xenopus laevis OX=8355 GN=ncs1 PE=2 SV=2)

HSP 1 Score: 47.8 bits (112), Expect = 1.3e-04
Identity = 33/109 (30.28%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 27  DSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDE 86
           D +KDG+I F+EF   L             S  S  + D   R  F + D DNDG+++  
Sbjct: 73  DENKDGRIEFSEFIQAL-------------SVTSRGTLDEKLRWAFKLYDLDNDGYITRN 132

Query: 87  ELLPIIEKIH---------PSEHYYAKQQAEYIIQQADADKDGRLTLTE 127
           E+L I++ I+         P E    +++ + I    D + DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNSDGKLTLQE 168

[Close]

BLAST of Lsi09G008530 vs. ExPASy Swiss-Prot
Match: P62167 (Neuronal calcium sensor 1 OS=Gallus gallus OX=9031 GN=NCS1 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 33/109 (30.28%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 27  DSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDE 86
           D +KDG+I F+EF   L             S  S  + D   R  F + D DNDG+++  
Sbjct: 73  DENKDGRIEFSEFIQAL-------------SVTSRGTLDEKLRWAFKLYDLDNDGYITRN 132

Query: 87  ELLPIIEKIH---------PSEHYYAKQQAEYIIQQADADKDGRLTLTE 127
           E+L I++ I+         P E    +++ + I    D + DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

[Close]

BLAST of Lsi09G008530 vs. ExPASy Swiss-Prot
Match: P62166 (Neuronal calcium sensor 1 OS=Homo sapiens OX=9606 GN=NCS1 PE=1 SV=2)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 33/109 (30.28%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 27  DSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDE 86
           D +KDG+I F+EF   L             S  S  + D   R  F + D DNDG+++  
Sbjct: 73  DENKDGRIEFSEFIQAL-------------SVTSRGTLDEKLRWAFKLYDLDNDGYITRN 132

Query: 87  ELLPIIEKIH---------PSEHYYAKQQAEYIIQQADADKDGRLTLTE 127
           E+L I++ I+         P E    +++ + I    D + DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

[Close]

BLAST of Lsi09G008530 vs. ExPASy Swiss-Prot
Match: Q8BNY6 (Neuronal calcium sensor 1 OS=Mus musculus OX=10090 GN=Ncs1 PE=1 SV=3)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 33/109 (30.28%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 27  DSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDE 86
           D +KDG+I F+EF   L             S  S  + D   R  F + D DNDG+++  
Sbjct: 73  DENKDGRIEFSEFIQAL-------------SVTSRGTLDEKLRWAFKLYDLDNDGYITRN 132

Query: 87  ELLPIIEKIH---------PSEHYYAKQQAEYIIQQADADKDGRLTLTE 127
           E+L I++ I+         P E    +++ + I    D + DG+LTL E
Sbjct: 133 EMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQE 168

[Close]

BLAST of Lsi09G008530 vs. ExPASy TrEMBL
Match: A0A5D3CVB0 (Calumenin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00460 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.3e-67
Identity = 128/130 (98.46%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 246 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 305

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           +EELLPII KIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE
Sbjct: 306 NEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 365

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 366 DDYDFHDEFR 375

[Close]

BLAST of Lsi09G008530 vs. ExPASy TrEMBL
Match: A0A1S3CNB9 (calumenin-B-like OS=Cucumis melo OX=3656 GN=LOC103502856 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 4.3e-67
Identity = 128/130 (98.46%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 246 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 305

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           +EELLPII KIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE
Sbjct: 306 NEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 365

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 366 DDYDFHDEFR 375

[Close]

BLAST of Lsi09G008530 vs. ExPASy TrEMBL
Match: A0A6J1FMS7 (calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.8e-66
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 250 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 309

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           DEELLPII KIHPSEHYYA+QQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE
Sbjct: 310 DEELLPIIGKIHPSEHYYAEQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 369

Query: 145 DDYDFHDEFR 155
           DDYD HDEFR
Sbjct: 370 DDYDSHDEFR 379

[Close]

BLAST of Lsi09G008530 vs. ExPASy TrEMBL
Match: A0A6J1JYP4 (calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111489489 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.3e-65
Identity = 125/130 (96.15%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSE+SRDGPARNLFAVLDKDNDGHLS
Sbjct: 250 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEESRDGPARNLFAVLDKDNDGHLS 309

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           DEELLPII KIHPSE+YYA+QQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE
Sbjct: 310 DEELLPIIGKIHPSENYYAEQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 369

Query: 145 DDYDFHDEFR 155
           DDYD HDEFR
Sbjct: 370 DDYDSHDEFR 379

[Close]

BLAST of Lsi09G008530 vs. ExPASy TrEMBL
Match: A0A6J1CJ22 (calumenin-like OS=Momordica charantia OX=3673 GN=LOC111011909 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.0e-63
Identity = 121/130 (93.08%), Postives = 126/130 (96.92%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFD+VRNYDENH+SSH S+D RDGPARNLFAVLDKDNDGHLS
Sbjct: 246 ERDSDKDGKINFNEFFHGLFDLVRNYDENHHSSHESDDPRDGPARNLFAVLDKDNDGHLS 305

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           DEELLPII KIHPSEHYYAKQQAEYI+QQADADKDGRLTL EMIDHPYVFYSAIFNED+E
Sbjct: 306 DEELLPIIGKIHPSEHYYAKQQAEYIVQQADADKDGRLTLAEMIDHPYVFYSAIFNEDEE 365

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 366 DDYDFHDEFR 375

[Close]

BLAST of Lsi09G008530 vs. NCBI nr
Match: XP_008465196.1 (PREDICTED: calumenin-B-like [Cucumis melo] >KAA0066135.1 calumenin-B-like [Cucumis melo var. makuwa] >TYK15178.1 calumenin-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 263.8 bits (673), Expect = 9.0e-67
Identity = 128/130 (98.46%), Postives = 129/130 (99.23%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 246 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 305

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           +EELLPII KIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE
Sbjct: 306 NEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 365

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 366 DDYDFHDEFR 375

[Close]

BLAST of Lsi09G008530 vs. NCBI nr
Match: KAE8648373.1 (hypothetical protein Csa_023160 [Cucumis sativus])

HSP 1 Score: 261.9 bits (668), Expect = 3.4e-66
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 213 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 272

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           +EELLPII KIHPSEHYYAKQQAEYIIQQADADKDGRLTL EMIDHPYVFYSAIFNEDDE
Sbjct: 273 NEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRLTLAEMIDHPYVFYSAIFNEDDE 332

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 333 DDYDFHDEFR 342

[Close]

BLAST of Lsi09G008530 vs. NCBI nr
Match: XP_031742276.1 (LOW QUALITY PROTEIN: calumenin-B [Cucumis sativus])

HSP 1 Score: 261.9 bits (668), Expect = 3.4e-66
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 246 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 305

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           +EELLPII KIHPSEHYYAKQQAEYIIQQADADKDGRLTL EMIDHPYVFYSAIFNEDDE
Sbjct: 306 NEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRLTLAEMIDHPYVFYSAIFNEDDE 365

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 366 DDYDFHDEFR 375

[Close]

BLAST of Lsi09G008530 vs. NCBI nr
Match: KAE8637521.1 (hypothetical protein CSA_004542 [Cucumis sativus] >KAE8637530.1 hypothetical protein CSA_022904 [Cucumis sativus])

HSP 1 Score: 261.9 bits (668), Expect = 3.4e-66
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 58  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 117

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           +EELLPII KIHPSEHYYAKQQAEYIIQQADADKDGRLTL EMIDHPYVFYSAIFNEDDE
Sbjct: 118 NEELLPIIGKIHPSEHYYAKQQAEYIIQQADADKDGRLTLAEMIDHPYVFYSAIFNEDDE 177

Query: 145 DDYDFHDEFR 155
           DDYDFHDEFR
Sbjct: 178 DDYDFHDEFR 187

[Close]

BLAST of Lsi09G008530 vs. NCBI nr
Match: XP_022939873.1 (calumenin-like [Cucurbita moschata] >KAG6579084.1 Calumenin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 261.2 bits (666), Expect = 5.8e-66
Identity = 127/130 (97.69%), Postives = 128/130 (98.46%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS
Sbjct: 250 ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 309

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 144
           DEELLPII KIHPSEHYYA+QQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE
Sbjct: 310 DEELLPIIGKIHPSEHYYAEQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDDE 369

Query: 145 DDYDFHDEFR 155
           DDYD HDEFR
Sbjct: 370 DDYDSHDEFR 379

[Close]

BLAST of Lsi09G008530 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 199.9 bits (507), Expect = 1.5e-51
Identity = 100/132 (75.76%), Postives = 115/132 (87.12%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDE-NHNSSHHSEDSRDGPARNLFAVLDKDNDGHL 84
           ERDSDKDGKI+F EFFHGLFD VRNY+E NHNS+H   D  +GPA+ LF+ LDK++DG+L
Sbjct: 260 ERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYHDLPEGPAKQLFSQLDKNDDGYL 319

Query: 85  SDEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNEDD 144
           SD ELLPII KIHP+EHYYAKQQA+YII QAD+DKD RLTL EMI+HPYVFYSAIF+EDD
Sbjct: 320 SDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRLTLAEMIEHPYVFYSAIFDEDD 379

Query: 145 -EDDYDFHDEFR 155
            +DDY FHDEFR
Sbjct: 380 TDDDYGFHDEFR 391

[Close]

BLAST of Lsi09G008530 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 85.5 bits (210), Expect = 4.1e-17
Identity = 48/127 (37.80%), Postives = 75/127 (59.06%), Query Frame = 0


Query: 27  DSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLSDE 86
           D++ DGK+ + EF    ++M + + +        ED      + LFA +D+D D  L  +
Sbjct: 222 DTNGDGKLEYKEFVKNAYEMYKEFAK----FEKEEDENVPTPQLLFAEMDRDKDRFLVAD 281

Query: 87  ELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMIDHPYVFYSAIFNED-DED 146
           EL PI++ + P E  YAK  + ++  +AD DKDG+L+L EM+ H  VFY A+ +ED D++
Sbjct: 282 ELRPILQYLQPGEMSYAKFYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDE 341

Query: 147 DYDFHDE 153
           DY  HDE
Sbjct: 342 DYFDHDE 344

[Close]

BLAST of Lsi09G008530 vs. TAIR 10
Match: AT4G37010.1 (centrin 2 )

HSP 1 Score: 41.6 bits (96), Expect = 6.8e-04
Identity = 28/104 (26.92%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           E D ++ G I+F+EF H +            +     DS D  ++  F ++D DN+G +S
Sbjct: 70  EVDKNQSGAIDFDEFVHMM-----------TTKFGERDSIDELSK-AFKIIDHDNNGKIS 129

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMI 129
             ++  I +++  +   +     E +I++AD DKDG + L E +
Sbjct: 130 PRDIKMIAKELGEN---FTDNDIEEMIEEADRDKDGEVNLEEFM 158

[Close]

BLAST of Lsi09G008530 vs. TAIR 10
Match: AT4G37010.2 (centrin 2 )

HSP 1 Score: 41.6 bits (96), Expect = 6.8e-04
Identity = 28/104 (26.92%), Postives = 52/104 (50.00%), Query Frame = 0


Query: 25  ERDSDKDGKINFNEFFHGLFDMVRNYDENHNSSHHSEDSRDGPARNLFAVLDKDNDGHLS 84
           E D ++ G I+F+EF H +            +     DS D  ++  F ++D DN+G +S
Sbjct: 74  EVDKNQSGAIDFDEFVHMM-----------TTKFGERDSIDELSK-AFKIIDHDNNGKIS 133

Query: 85  DEELLPIIEKIHPSEHYYAKQQAEYIIQQADADKDGRLTLTEMI 129
             ++  I +++  +   +     E +I++AD DKDG + L E +
Sbjct: 134 PRDIKMIAKELGEN---FTDNDIEEMIEEADRDKDGEVNLEEFM 162

The following BLAST results are available for this feature:

BLAST of Lsi09G008530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9JM83	2.0e-05	31.37	Calmodulin-4 OS=Mus musculus OX=10090 GN=Calm4 PE=2 SV=2	[more]
Q91614	1.3e-04	30.28	Neuronal calcium sensor 1 OS=Xenopus laevis OX=8355 GN=ncs1 PE=2 SV=2	[more]
P62167	1.7e-04	30.28	Neuronal calcium sensor 1 OS=Gallus gallus OX=9031 GN=NCS1 PE=1 SV=2	[more]
P62166	1.7e-04	30.28	Neuronal calcium sensor 1 OS=Homo sapiens OX=9606 GN=NCS1 PE=1 SV=2	[more]
Q8BNY6	1.7e-04	30.28	Neuronal calcium sensor 1 OS=Mus musculus OX=10090 GN=Ncs1 PE=1 SV=3	[more]

BLAST of Lsi09G008530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CVB0	4.3e-67	98.46	Calumenin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G004...	[more]
A0A1S3CNB9	4.3e-67	98.46	calumenin-B-like OS=Cucumis melo OX=3656 GN=LOC103502856 PE=4 SV=1	[more]
A0A6J1FMS7	2.8e-66	97.69	calumenin-like OS=Cucurbita moschata OX=3662 GN=LOC111445607 PE=4 SV=1	[more]
A0A6J1JYP4	5.3e-65	96.15	calumenin-like OS=Cucurbita maxima OX=3661 GN=LOC111489489 PE=4 SV=1	[more]
A0A6J1CJ22	1.0e-63	93.08	calumenin-like OS=Momordica charantia OX=3673 GN=LOC111011909 PE=4 SV=1	[more]

BLAST of Lsi09G008530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008465196.1	9.0e-67	98.46	PREDICTED: calumenin-B-like [Cucumis melo] >KAA0066135.1 calumenin-B-like [Cucum...	[more]
KAE8648373.1	3.4e-66	97.69	hypothetical protein Csa_023160 [Cucumis sativus]	[more]
XP_031742276.1	3.4e-66	97.69	LOW QUALITY PROTEIN: calumenin-B [Cucumis sativus]	[more]
KAE8637521.1	3.4e-66	97.69	hypothetical protein CSA_004542 [Cucumis sativus] >KAE8637530.1 hypothetical pro...	[more]
XP_022939873.1	5.8e-66	97.69	calumenin-like [Cucurbita moschata] >KAG6579084.1 Calumenin, partial [Cucurbita ...	[more]

BLAST of Lsi09G008530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08580.1	1.5e-51	75.76	Calcium-binding EF hand family protein	[more]
AT4G27790.1	4.1e-17	37.80	Calcium-binding EF hand family protein	[more]
AT4G37010.1	6.8e-04	26.92	centrin 2	[more]
AT4G37010.2	6.8e-04	26.92	centrin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 2..67 e-value: 1.3E-7 score: 33.7
None	No IPR available	GENE3D	1.10.238.10		coord: 68..130 e-value: 5.8E-12 score: 47.6
None	No IPR available	PANTHER	PTHR10827:SF83	BNAC09G47400D PROTEIN	coord: 25..154
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 25..154
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 70..128 e-value: 3.4E-10 score: 40.2
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 25..44 e-value: 0.013 score: 15.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 27..49 score: 8.487999
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 63..98 score: 10.552258
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 102..137 score: 9.854874
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 27..39
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 76..88
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 115..127
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 27..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G008530.1	Lsi09G008530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding