Lsi09G008490 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G008490
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Eukaryotic aspartyl protease family protein
Location	chr09: 9777950 .. 9779849 (-)
RNA-Seq Expression	Lsi09G008490
Synteny	Lsi09G008490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCAGCTTCTTATCTATTTCTTTGTACAGAAAATACTCACAAACCAGTAACTCAATCATAATCCAAACCCCACAATTCTCTTTTTCCCTCTCATATTCCCTCCCACACTTAAATCCGCCATTTCTCATTTTCATTCTCAATCTCCCTTACAACACTCACTTCACAGCTCAACTACTAAACCATGTTACCTTTCCTTCTCCTTCTTCCTCTTTTAGCCACCACTGTCTCCCCGGTTGCCACCGGTCCAGCCGCTACTTACCCGGCCACCCAGCTCCTAAATGTCAAAGACACAATCAAAGAAACAGAAACTAGGCCATCTAGACTACCACAAGATCTTGACCTCCATGAAAACTACCCTCCTTTTGACAATAGCAGCAGTCAGAGTCCATGGAAGCTCAAGCTCTTCCATAGAGATAAGCTGCCGCTCAACTTCGACCCCGACCATAGTCGTCGTTTCAAGGAGCGCATTGAAAGAGATTCCAAAAGGGTCTCCTCTCTGCTCCGCCAACTCTCCAATGGCAGCGACGAGCAGGTGATCAGGGGTTAAGCTAAGAAGGAAGGAGGGAGGCAATTTCCCTCCTTTGAATTTTTATAAACATACTCAATATTGTTTTCAAGATTATATATAAAAAATGTTGGAATGGAAATTTTTTGTTTCTTTCTGATTTTATTTCTTTCTTTTTTGGTTGTGGCTCTGCAGGTGACGGACTTCGGGTCGGACGTGGTCTCCGGCACGGAGCAAGGGAGCGGAGAGTACTTTGTGAGGATCGGCGTCGGCAGCCCGCCGAGGAGCCAATACGTCGTGATTGATTCCGGCAGCGACATTGTTTGGGTGCAATGCCAGCCCTGTAGCGAATGCTACCAACAGTCCGACCCGGTGTTTGATCCGGCCGGTTCCGCCTCTTACGCCGGAATCTCCTGCGACTCGTCAGTGTGCGACCGCCTCGACAACGCAGGCTGCAACGAAGGCCGGTGCCGGTACGAGGTGTCGTACGGCGACGGATCCTACACCCGCGGAACACTCGCGCTTGAAACCCTAACTTTCGGGCGGGTCGTAATCCGAAACATCGCGATCGGGTGCGGCCATATGAACCGAGGAATGTTCGTCGGAGCCGCAGGGTTACTCGGCCTCGGCGGTGGCGCCATGTCGTTCGTCGGCCAACTCGGCGGCCAGACTGGCGGCGCGTTCAGTTACTGTTTGGTCAGTCGAGGCACCGAGTCCACTGGAACGCTGGAGTTCGGCCGCGGTGCTATGCCAGTTGGCGGCGCGTGGGTTCCACTAATCCGAAACCCACGCGCTCCAAGTTTCTACTACGTCGGGCTTTCAGGACTGGGAGTCGGAGGGATCCGAGTTCCAATACCCGAACAGATCTTCGAACTCACCGATCTAGGGTACGGTGGCGTGGTGATGGACACCGGAACCGCCGTGACGAGGCTACCGGCGCCGGCGTACGAAGCACTCCGAGACACCTTCATCGGACAAACGGCAAACTTGCCTCGATCGGGCAGAGTATCGATCTTCGACACATGCTATAACCTAAACGGGTTCGTATCGGTAAGGGTACCGACGGTGTCGTTCTACTTCTCCGGCGGGCCGATACTGACGTTGCCGGCGAGGAACTTTCTGATTCCGGTGGACGGCGAAGGGACATTTTGCTTCGCATTTGCAGCATCAGCGTCGGGATTGTCGATAATAGGAAACATTCAGCAAGAAGGAATTCAAATCTCCATTGATGGATCAAATGGGTTTGTGGGATTTGGGCCAAGTATTTGTTAATTCGTCAAATATTAAAGGGCAAATCTGTCATTCTGCTAAATACTAGTTTTCTACTTATATTATTATATTTCATTTTTTCCCCAAATTTTAAAAGTATATATATCTTATAGAAGTTTATAGAA

mRNA sequence

CTTCAGCTTCTTATCTATTTCTTTGTACAGAAAATACTCACAAACCAGTAACTCAATCATAATCCAAACCCCACAATTCTCTTTTTCCCTCTCATATTCCCTCCCACACTTAAATCCGCCATTTCTCATTTTCATTCTCAATCTCCCTTACAACACTCACTTCACAGCTCAACTACTAAACCATGTTACCTTTCCTTCTCCTTCTTCCTCTTTTAGCCACCACTGTCTCCCCGGTTGCCACCGGTCCAGCCGCTACTTACCCGGCCACCCAGCTCCTAAATGTCAAAGACACAATCAAAGAAACAGAAACTAGGCCATCTAGACTACCACAAGATCTTGACCTCCATGAAAACTACCCTCCTTTTGACAATAGCAGCAGTCAGAGTCCATGGAAGCTCAAGCTCTTCCATAGAGATAAGCTGCCGCTCAACTTCGACCCCGACCATAGTCGTCGTTTCAAGGAGCGCATTGAAAGAGATTCCAAAAGGGTCTCCTCTCTGCTCCGCCAACTCTCCAATGGCAGCGACGAGCAGGTGACGGACTTCGGGTCGGACGTGGTCTCCGGCACGGAGCAAGGGAGCGGAGAGTACTTTGTGAGGATCGGCGTCGGCAGCCCGCCGAGGAGCCAATACGTCGTGATTGATTCCGGCAGCGACATTGTTTGGGTGCAATGCCAGCCCTGTAGCGAATGCTACCAACAGTCCGACCCGGTGTTTGATCCGGCCGGTTCCGCCTCTTACGCCGGAATCTCCTGCGACTCGTCAGTGTGCGACCGCCTCGACAACGCAGGCTGCAACGAAGGCCGGTGCCGGTACGAGGTGTCGTACGGCGACGGATCCTACACCCGCGGAACACTCGCGCTTGAAACCCTAACTTTCGGGCGGGTCGTAATCCGAAACATCGCGATCGGGTGCGGCCATATGAACCGAGGAATGTTCGTCGGAGCCGCAGGGTTACTCGGCCTCGGCGGTGGCGCCATGTCGTTCGTCGGCCAACTCGGCGGCCAGACTGGCGGCGCGTTCAGTTACTGTTTGGTCAGTCGAGGCACCGAGTCCACTGGAACGCTGGAGTTCGGCCGCGGTGCTATGCCAGTTGGCGGCGCGTGGGTTCCACTAATCCGAAACCCACGCGCTCCAAGTTTCTACTACGTCGGGCTTTCAGGACTGGGAGTCGGAGGGATCCGAGTTCCAATACCCGAACAGATCTTCGAACTCACCGATCTAGGGTACGGTGGCGTGGTGATGGACACCGGAACCGCCGTGACGAGGCTACCGGCGCCGGCGTACGAAGCACTCCGAGACACCTTCATCGGACAAACGGCAAACTTGCCTCGATCGGGCAGAGTATCGATCTTCGACACATGCTATAACCTAAACGGGTTCGTATCGGTAAGGGTACCGACGGTGTCGTTCTACTTCTCCGGCGGGCCGATACTGACGTTGCCGGCGAGGAACTTTCTGATTCCGGTGGACGGCGAAGGGACATTTTGCTTCGCATTTGCAGCATCAGCGTCGGGATTGTCGATAATAGGAAACATTCAGCAAGAAGGAATTCAAATCTCCATTGATGGATCAAATGGGTTTGTGGGATTTGGGCCAAGTATTTGTTAATTCGTCAAATATTAAAGGGCAAATCTGTCATTCTGCTAAATACTAGTTTTCTACTTATATTATTATATTTCATTTTTTCCCCAAATTTTAAAAGTATATATATCTTATAGAAGTTTATAGAA

Coding sequence (CDS)

ATGTTACCTTTCCTTCTCCTTCTTCCTCTTTTAGCCACCACTGTCTCCCCGGTTGCCACCGGTCCAGCCGCTACTTACCCGGCCACCCAGCTCCTAAATGTCAAAGACACAATCAAAGAAACAGAAACTAGGCCATCTAGACTACCACAAGATCTTGACCTCCATGAAAACTACCCTCCTTTTGACAATAGCAGCAGTCAGAGTCCATGGAAGCTCAAGCTCTTCCATAGAGATAAGCTGCCGCTCAACTTCGACCCCGACCATAGTCGTCGTTTCAAGGAGCGCATTGAAAGAGATTCCAAAAGGGTCTCCTCTCTGCTCCGCCAACTCTCCAATGGCAGCGACGAGCAGGTGACGGACTTCGGGTCGGACGTGGTCTCCGGCACGGAGCAAGGGAGCGGAGAGTACTTTGTGAGGATCGGCGTCGGCAGCCCGCCGAGGAGCCAATACGTCGTGATTGATTCCGGCAGCGACATTGTTTGGGTGCAATGCCAGCCCTGTAGCGAATGCTACCAACAGTCCGACCCGGTGTTTGATCCGGCCGGTTCCGCCTCTTACGCCGGAATCTCCTGCGACTCGTCAGTGTGCGACCGCCTCGACAACGCAGGCTGCAACGAAGGCCGGTGCCGGTACGAGGTGTCGTACGGCGACGGATCCTACACCCGCGGAACACTCGCGCTTGAAACCCTAACTTTCGGGCGGGTCGTAATCCGAAACATCGCGATCGGGTGCGGCCATATGAACCGAGGAATGTTCGTCGGAGCCGCAGGGTTACTCGGCCTCGGCGGTGGCGCCATGTCGTTCGTCGGCCAACTCGGCGGCCAGACTGGCGGCGCGTTCAGTTACTGTTTGGTCAGTCGAGGCACCGAGTCCACTGGAACGCTGGAGTTCGGCCGCGGTGCTATGCCAGTTGGCGGCGCGTGGGTTCCACTAATCCGAAACCCACGCGCTCCAAGTTTCTACTACGTCGGGCTTTCAGGACTGGGAGTCGGAGGGATCCGAGTTCCAATACCCGAACAGATCTTCGAACTCACCGATCTAGGGTACGGTGGCGTGGTGATGGACACCGGAACCGCCGTGACGAGGCTACCGGCGCCGGCGTACGAAGCACTCCGAGACACCTTCATCGGACAAACGGCAAACTTGCCTCGATCGGGCAGAGTATCGATCTTCGACACATGCTATAACCTAAACGGGTTCGTATCGGTAAGGGTACCGACGGTGTCGTTCTACTTCTCCGGCGGGCCGATACTGACGTTGCCGGCGAGGAACTTTCTGATTCCGGTGGACGGCGAAGGGACATTTTGCTTCGCATTTGCAGCATCAGCGTCGGGATTGTCGATAATAGGAAACATTCAGCAAGAAGGAATTCAAATCTCCATTGATGGATCAAATGGGTTTGTGGGATTTGGGCCAAGTATTTGTTAA

Protein sequence

MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPPFDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC

Homology

[Close]

BLAST of Lsi09G008490 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 3.3e-179
Identity = 303/438 (69.18%), Postives = 360/438 (82.19%), Query Frame = 0


Query: 48  LPQDLDLHENYPPFDNS----SSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRV 107
           L   L +    P F+N+     S S + L+L HRD+ P     +H  R   R+ RD+ RV
Sbjct: 33  LQPPLTVTATLPDFNNTHFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRV 92

Query: 108 SSLLRQL------SNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGS 167
           S++LR++      S+ S  +V DFGSD+VSG +QGSGEYFVRIGVGSPPR QY+VIDSGS
Sbjct: 93  SAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGS 152

Query: 168 DIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGD 227
           D+VWVQCQPC  CY+QSDPVFDPA S SY G+SC SSVCDR++N+GC+ G CRYEV YGD
Sbjct: 153 DMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGD 212

Query: 228 GSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTG 287
           GSYT+GTLALETLTF + V+RN+A+GCGH NRGMF+GAAGLLG+GGG+MSFVGQL GQTG
Sbjct: 213 GSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTG 272

Query: 288 GAFSYCLVSRGTESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPI 347
           GAF YCLVSRGT+STG+L FGR A+PVG +WVPL+RNPRAPSFYYVGL GLGVGG+R+P+
Sbjct: 273 GAFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPL 332

Query: 348 PEQIFELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNL 407
           P+ +F+LT+ G GGVVMDTGTAVTRLP  AY A RD F  QTANLPR+  VSIFDTCY+L
Sbjct: 333 PDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDL 392

Query: 408 NGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGI 467
           +GFVSVRVPTVSFYF+ GP+LTLPARNFL+PVD  GT+CFAFAAS +GLSIIGNIQQEGI
Sbjct: 393 SGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGI 452

Query: 468 QISIDGSNGFVGFGPSIC 476
           Q+S DG+NGFVGFGP++C
Sbjct: 453 QVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Lsi09G008490 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 4.5e-112
Identity = 223/500 (44.60%), Postives = 311/500 (62.20%), Query Frame = 0


Query: 4   FLLLLPL----LATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYP 63
           FL LL +    L  T +  ++   +T P T +L+V  ++++T+T  S  P    L    P
Sbjct: 6   FLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKP 65

Query: 64  P------FDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLS-- 123
                  F NSS  SP  L+L  RD    +   D+      R+ERDS RV+ ++ ++   
Sbjct: 66  ESLSDPVFFNSS--SPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 125

Query: 124 -NGSDE-------------QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGS 183
             G D              Q  D  + VVSG  QGSGEYF RIGVG+P +  Y+V+D+GS
Sbjct: 126 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 185

Query: 184 DIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGD 243
           D+ W+QC+PC++CYQQSDPVF+P  S++Y  ++C +  C  L+ + C   +C Y+VSYGD
Sbjct: 186 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 245

Query: 244 GSYTRGTLALETLTFGRV-VIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQT 303
           GS+T G LA +T+TFG    I N+A+GCGH N G+F GAAGLLGLGGG +S   Q+    
Sbjct: 246 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---K 305

Query: 304 GGAFSYCLVSRGTESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP 363
             +FSYCLV R +  + +L+F    +  G A  PL+RN +  +FYYVGLSG  VGG +V 
Sbjct: 306 ATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVV 365

Query: 364 IPEQIFELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPR-SGRVSIFDTCY 423
           +P+ IF++   G GGV++D GTAVTRL   AY +LRD F+  T NL + S  +S+FDTCY
Sbjct: 366 LPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCY 425

Query: 424 NLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQE 476
           + +   +V+VPTV+F+F+GG  L LPA+N+LIPVD  GTFCFAFA ++S LSIIGN+QQ+
Sbjct: 426 DFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQ 485

[Close]

BLAST of Lsi09G008490 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.6e-109
Identity = 215/432 (49.77%), Postives = 274/432 (63.43%), Query Frame = 0


Query: 54  LHENYPPFDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNG 113
           L   +    +S S S   L L H D L  N  PD    F  R++RDS+RV S+    +  
Sbjct: 56  LESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQI 115

Query: 114 SDEQVT------DFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPC 173
               VT       F S VVSG  QGSGEYF R+GVG+P R  Y+V+D+GSDIVW+QC PC
Sbjct: 116 PGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC 175

Query: 174 SECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGR--CRYEVSYGDGSYTRGTL 233
             CY QSDP+FDP  S +YA I C S  C RLD+AGCN  R  C Y+VSYGDGS+T G  
Sbjct: 176 RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDF 235

Query: 234 ALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLV 293
           + ETLTF R  ++ +A+GCGH N G+FVGAAGLLGLG G +SF GQ G +    FSYCLV
Sbjct: 236 STETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLV 295

Query: 294 SRGTES-TGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP-IPEQIFE 353
            R   S   ++ FG  A+     + PL+ NP+  +FYYVGL G+ VGG RVP +   +F+
Sbjct: 296 DRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFK 355

Query: 354 LTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSV 413
           L  +G GGV++D+GT+VTRL  PAY A+RD F      L R+   S+FDTC++L+    V
Sbjct: 356 LDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEV 415

Query: 414 RVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDG 473
           +VPTV  +F G  + +LPA N+LIPVD  G FCFAFA +  GLSIIGNIQQ+G ++  D 
Sbjct: 416 KVPTVVLHFRGADV-SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDL 475

Query: 474 SNGFVGFGPSIC 476
           ++  VGF P  C
Sbjct: 476 ASSRVGFAPGGC 484

[Close]

BLAST of Lsi09G008490 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.4e-67
Identity = 150/389 (38.56%), Postives = 215/389 (55.27%), Query Frame = 0


Query: 93  KERIERDSKRVSSLLRQLSNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVV 152
           K  I+R  +R+ S+   L + S  +   +          G GEY + + +G+P  S   +
Sbjct: 62  KRAIKRGERRMRSINAMLQSSSGIETPVYA---------GDGEYLMNVAIGTPDSSFSAI 121

Query: 153 IDSGSDIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYE 212
           +D+GSD++W QC+PC++C+ Q  P+F+P  S+S++ + C+S  C  L +  CN   C+Y 
Sbjct: 122 MDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYT 181

Query: 213 VSYGDGSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVG-AAGLLGLGGGAMSFVGQ 272
             YGDGS T+G +A ET TF    + NIA GCG  N+G   G  AGL+G+G G +S   Q
Sbjct: 182 YGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQ 241

Query: 273 LGGQTGGAFSYCLVSRGTESTGTLEFGRGA--MPVGGAWVPLIRNPRAPSFYYVGLSGLG 332
           LG    G FSYC+ S G+ S  TL  G  A  +P G     LI +   P++YY+ L G+ 
Sbjct: 242 LG---VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGIT 301

Query: 333 VGGIRVPIPEQIFELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVS 392
           VGG  + IP   F+L D G GG+++D+GT +T LP  AY A+   F  Q  NLP     S
Sbjct: 302 VGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQ-INLPTVDESS 361

Query: 393 I-FDTCYNL-NGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASAS-GL 452
               TC+   +   +V+VP +S  F GG +L L  +N LI    EG  C A  +S+  G+
Sbjct: 362 SGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAEGVICLAMGSSSQLGI 421

Query: 453 SIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           SI GNIQQ+  Q+  D  N  V F P+ C
Sbjct: 422 SIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Lsi09G008490 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 5.8e-67
Identity = 146/386 (37.82%), Postives = 218/386 (56.48%), Query Frame = 0


Query: 96  IERDSKRVSSLLRQLSNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDS 155
           IER S+R+  L         E + +  S V +    G GEY + + +G+P +    ++D+
Sbjct: 64  IERGSRRLQRL---------EAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDT 123

Query: 156 GSDIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSY 215
           GSD++W QCQPC++C+ QS P+F+P GS+S++ + C S +C  L +  C+   C+Y   Y
Sbjct: 124 GSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGY 183

Query: 216 GDGSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVG-AAGLLGLGGGAMSFVGQLGG 275
           GDGS T+G++  ETLTFG V I NI  GCG  N+G   G  AGL+G+G G +S   QL  
Sbjct: 184 GDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV 243

Query: 276 QTGGAFSYCLVSRGTESTGTLEFG--RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGG 335
                FSYC+   G+ +   L  G    ++  G     LI++ + P+FYY+ L+GL VG 
Sbjct: 244 T---KFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGS 303

Query: 336 IRVPIPEQIFEL-TDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLP-RSGRVSI 395
            R+PI    F L ++ G GG+++D+GT +T     AY+++R  FI Q  NLP  +G  S 
Sbjct: 304 TRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ-INLPVVNGSSSG 363

Query: 396 FDTCYNLNGFVS-VRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSII 455
           FD C+      S +++PT   +F GG  L LP+ N+ I     G  C A  +S+ G+SI 
Sbjct: 364 FDLCFQTPSDPSNLQIPTFVMHFDGGD-LELPSENYFIS-PSNGLICLAMGSSSQGMSIF 423

Query: 456 GNIQQEGIQISIDGSNGFVGFGPSIC 476
           GNIQQ+ + +  D  N  V F  + C
Sbjct: 424 GNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Lsi09G008490 vs. ExPASy TrEMBL
Match: A0A5A7VGC3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00380 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.7e-259
Identity = 452/477 (94.76%), Postives = 460/477 (96.44%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLP LLLLPLLAT VS VATGPAATYP TQLLNVKDTIKETET PSRLPQDLDLHENYP 
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPVTQLLNVKDTIKETETTPSRLPQDLDLHENYPL 60

Query: 61  F--DNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQV 120
           F  DN+SSQS WKLKLFHRDKLPLNFD +H RRFKERI RDSKRVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 181 DPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 240
           DPAGSA+YAGISCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 241 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 301 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 361 AVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420
           AVTRLPAPAYEA RDTFIGQTANLPRS RVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lsi09G008490 vs. ExPASy TrEMBL
Match: A0A1S3CNF7 (protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo OX=3656 GN=LOC103502903 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.4e-259
Identity = 452/477 (94.76%), Postives = 461/477 (96.65%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLP LLLLPLLAT VS VATGPAATYPATQLLNVKDTIKETET PSRLPQDL+LHENYP 
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPATQLLNVKDTIKETETTPSRLPQDLNLHENYPL 60

Query: 61  F--DNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQV 120
           F  DN+SSQS WKLKLFHRDKLPLNFD +H RRFKERI RDSKRVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 181 DPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 240
           DPAGSA+YAGISCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 241 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 301 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 361 AVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420
           AVTRLPAPAYEA RDTFIGQTANLPRS RVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lsi09G008490 vs. ExPASy TrEMBL
Match: A0A6J1E0R8 (protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucurbita moschata OX=3662 GN=LOC111429766 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.0e-247
Identity = 435/480 (90.62%), Postives = 451/480 (93.96%), Query Frame = 0


Query: 1   MLPFLLLLPLL-----ATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLH 60
           MLPFLLLL LL        V  VA+  AA+YPATQLL+VKDTIKETE +PSRLPQDL+LH
Sbjct: 1   MLPFLLLLLLLPPLLTRAAVDTVASSLAASYPATQLLHVKDTIKETEIKPSRLPQDLELH 60

Query: 61  ENYPPFDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSD 120
           ENYPP DN S+Q+ WKL+LFHRDKLPLNFDPDH RRFKERI RD +RVSSLLR+LSNGSD
Sbjct: 61  ENYPPIDN-STQNQWKLELFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRRLSNGSD 120

Query: 121 EQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSD 180
           EQVTDFGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSD
Sbjct: 121 EQVTDFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSD 180

Query: 181 PVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRV 240
           PVFDPA SASYAGISCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV
Sbjct: 181 PVFDPASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRV 240

Query: 241 VIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTL 300
           +IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTL
Sbjct: 241 LIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTL 300

Query: 301 EFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMD 360
           EFGR AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMD
Sbjct: 301 EFGRSAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMD 360

Query: 361 TGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGG 420
           TGTAVTRLP PAYEA RDTFIGQTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGG
Sbjct: 361 TGTAVTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGG 420

Query: 421 PILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           PILTLPARNFLIPVDGEGTFCFAFAAS SGLSIIGNIQQEGIQIS+DG+NGFVGFGPSIC
Sbjct: 421 PILTLPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 479

[Close]

BLAST of Lsi09G008490 vs. ExPASy TrEMBL
Match: A0A6J1JNZ5 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487042 PE=3 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 1.3e-247
Identity = 435/479 (90.81%), Postives = 452/479 (94.36%), Query Frame = 0


Query: 1   MLPFLLLL---PLLA-TTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHE 60
           MLPFLLLL   PLL    +  VATG AA YPATQLL+VKDTIKETE +PSRLPQDL+L+E
Sbjct: 1   MLPFLLLLLLPPLLTRAAIDTVATGLAANYPATQLLHVKDTIKETEIKPSRLPQDLELYE 60

Query: 61  NYPPFDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDE 120
           NYPP DN S+Q+ WKL+LFHRDKLPLNFDPDH RRFKERI RD +RVSSLLR+LSNGSDE
Sbjct: 61  NYPPIDN-STQNQWKLELFHRDKLPLNFDPDHCRRFKERIGRDVERVSSLLRRLSNGSDE 120

Query: 121 QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180
           QVT+FGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP
Sbjct: 121 QVTEFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180

Query: 181 VFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVV 240
           VFDPA SASYAGISCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV+
Sbjct: 181 VFDPASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRVL 240

Query: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE 300
           IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE
Sbjct: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE 300

Query: 301 FGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT 360
           FGR AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT
Sbjct: 301 FGRSAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT 360

Query: 361 GTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGP 420
           GTAVTRLP PAYEA RDTFIGQTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGP
Sbjct: 361 GTAVTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGGP 420

Query: 421 ILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           ILTLPARNFLIPVDGEGTFCFAFAAS SGLSIIGNIQQEGIQIS+DG+NGFVGFGPSIC
Sbjct: 421 ILTLPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 478

[Close]

BLAST of Lsi09G008490 vs. ExPASy TrEMBL
Match: A0A6J1CII3 (protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Momordica charantia OX=3673 GN=LOC111011806 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.3e-242
Identity = 423/475 (89.05%), Postives = 442/475 (93.05%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLPFLL LPLL  TV+       A YP TQLLNVKD  KETE+ P+RLP+DL LHENY P
Sbjct: 5   MLPFLLFLPLLTATVA-----ATAAYPVTQLLNVKDITKETESEPTRLPEDLPLHENY-P 64

Query: 61  FDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQVTD 120
             N+++ + WKLKLFHRD LPLNFDPDH  RFKER+ERD KRVSSLLRQLSN S+EQVTD
Sbjct: 65  IHNTTTHTQWKLKLFHRDNLPLNFDPDHRCRFKERMERDLKRVSSLLRQLSNDSNEQVTD 124

Query: 121 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDP 180
           FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCS+CYQQSDPVFDP
Sbjct: 125 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSKCYQQSDPVFDP 184

Query: 181 AGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNI 240
           AGSASYAG+SCDSSVCDRLDNAGCN+GRCRYEV+YGDGSYT GTLALETLTFGRV+IRNI
Sbjct: 185 AGSASYAGVSCDSSVCDRLDNAGCNDGRCRYEVAYGDGSYTSGTLALETLTFGRVMIRNI 244

Query: 241 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRG 300
           AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTG+LEFGRG
Sbjct: 245 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGSLEFGRG 304

Query: 301 AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAV 360
           AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGG RVPIPEQIFELT+LGYGGVVMDTGTAV
Sbjct: 305 AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGTRVPIPEQIFELTNLGYGGVVMDTGTAV 364

Query: 361 TRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPILTL 420
           TRLPAPAYEA RDTFIG TANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGPILTL
Sbjct: 365 TRLPAPAYEAFRDTFIGLTANLPRSAGVSIFDTCYDLNGFVSVRVPTVSFYFSGGPILTL 424

Query: 421 PARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           PARNFLIPVDGEGTFCFAFAAS SGLSIIGNIQQEGIQISIDG+NGFVGFGPSIC
Sbjct: 425 PARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISIDGANGFVGFGPSIC 473

[Close]

BLAST of Lsi09G008490 vs. NCBI nr
Match: XP_038875928.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Benincasa hispida])

HSP 1 Score: 919.8 bits (2376), Expect = 9.3e-264
Identity = 458/475 (96.42%), Postives = 465/475 (97.89%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLPFLLLLPLLAT +S V+T PAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP
Sbjct: 1   MLPFLLLLPLLATAISLVSTSPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60

Query: 61  FDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQVTD 120
           FDNSSSQS WKLKLFHRDKLP NF+ DH RRFKERIERDS RVSSLLR LSNGSDEQVTD
Sbjct: 61  FDNSSSQSQWKLKLFHRDKLPFNFNTDHPRRFKERIERDSIRVSSLLRLLSNGSDEQVTD 120

Query: 121 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDP 180
           FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPC+ECYQQSDPVFDP
Sbjct: 121 FGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCNECYQQSDPVFDP 180

Query: 181 AGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNI 240
           AGSASYAGISCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGRV+IRNI
Sbjct: 181 AGSASYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRVIIRNI 240

Query: 241 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRG 300
           AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRG
Sbjct: 241 AIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRG 300

Query: 301 AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAV 360
           AMPVGGAWVPLIRNPRAPSFYYV LSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAV
Sbjct: 301 AMPVGGAWVPLIRNPRAPSFYYVRLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAV 360

Query: 361 TRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPILTL 420
           TRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGF+SVRVPTVSFYFSGGPILTL
Sbjct: 361 TRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFISVRVPTVSFYFSGGPILTL 420

Query: 421 PARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           PARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC
Sbjct: 421 PARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 475

[Close]

BLAST of Lsi09G008490 vs. NCBI nr
Match: KAA0066127.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo var. makuwa] >TYK15170.1 protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo var. makuwa])

HSP 1 Score: 902.9 bits (2332), Expect = 1.2e-258
Identity = 452/477 (94.76%), Postives = 460/477 (96.44%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLP LLLLPLLAT VS VATGPAATYP TQLLNVKDTIKETET PSRLPQDLDLHENYP 
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPVTQLLNVKDTIKETETTPSRLPQDLDLHENYPL 60

Query: 61  F--DNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQV 120
           F  DN+SSQS WKLKLFHRDKLPLNFD +H RRFKERI RDSKRVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 181 DPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 240
           DPAGSA+YAGISCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 241 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 301 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 361 AVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420
           AVTRLPAPAYEA RDTFIGQTANLPRS RVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lsi09G008490 vs. NCBI nr
Match: XP_008465249.1 (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo])

HSP 1 Score: 902.5 bits (2331), Expect = 1.5e-258
Identity = 452/477 (94.76%), Postives = 461/477 (96.65%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLP LLLLPLLAT VS VATGPAATYPATQLLNVKDTIKETET PSRLPQDL+LHENYP 
Sbjct: 1   MLPLLLLLPLLATAVSSVATGPAATYPATQLLNVKDTIKETETTPSRLPQDLNLHENYPL 60

Query: 61  F--DNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQV 120
           F  DN+SSQS WKLKLFHRDKLPLNFD +H RRFKERI RDSKRVSSLLR LSN SDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDTNHPRRFKERISRDSKRVSSLLRLLSNASDEQV 120

Query: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 181 DPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 240
           DPAGSA+YAGISCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGR++IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRILIR 240

Query: 241 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 301 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 361 AVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420
           AVTRLPAPAYEA RDTFIGQTANLPRS RVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 477

[Close]

BLAST of Lsi09G008490 vs. NCBI nr
Match: XP_004150193.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KAE8648376.1 hypothetical protein Csa_023356 [Cucumis sativus])

HSP 1 Score: 899.0 bits (2322), Expect = 1.7e-257
Identity = 452/477 (94.76%), Postives = 461/477 (96.65%), Query Frame = 0


Query: 1   MLPFLLLLPLLATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYPP 60
           MLPF LLL LLAT V+ VATGPAATYPATQLLNVKDTIKE ET PSRLPQDL+LHENYP 
Sbjct: 1   MLPF-LLLSLLATAVASVATGPAATYPATQLLNVKDTIKEAETAPSRLPQDLELHENYPI 60

Query: 61  F--DNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDEQV 120
           F  DN+SSQS WKLKLFHRDKLPLNFDPDH RRFKERI RDSKRVSSLLR LS+GSDEQV
Sbjct: 61  FELDNNSSQSQWKLKLFHRDKLPLNFDPDHPRRFKERISRDSKRVSSLLRLLSSGSDEQV 120

Query: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180
           TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF
Sbjct: 121 TDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVF 180

Query: 181 DPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIR 240
           DPAGSA+YAGISCDSSVCDRLDNAGCN+GRCRYEVSYGDGSYTRGTLALETLTFGRV+IR
Sbjct: 181 DPAGSATYAGISCDSSVCDRLDNAGCNDGRCRYEVSYGDGSYTRGTLALETLTFGRVLIR 240

Query: 241 NIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300
           NIAIGCGHMNRGMF+GAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG
Sbjct: 241 NIAIGCGHMNRGMFIGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFG 300

Query: 301 RGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360
           RGAMPVG AWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT
Sbjct: 301 RGAMPVGAAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGT 360

Query: 361 AVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420
           AVTRLPAPAYEA RDTFIGQTANLPRS RVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL
Sbjct: 361 AVTRLPAPAYEAFRDTFIGQTANLPRSDRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPIL 420

Query: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGP+IC
Sbjct: 421 TLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPTIC 476

[Close]

BLAST of Lsi09G008490 vs. NCBI nr
Match: KAG7032671.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 867.8 bits (2241), Expect = 4.2e-248
Identity = 437/479 (91.23%), Postives = 452/479 (94.36%), Query Frame = 0


Query: 1   MLPFLLLL---PLLA-TTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHE 60
           MLPFLLLL   PLL    V  VA+G AA+YPATQLL+VKDTIKETE +PSRLPQDL+LHE
Sbjct: 1   MLPFLLLLLLPPLLTRAAVDTVASGLAASYPATQLLHVKDTIKETEIKPSRLPQDLELHE 60

Query: 61  NYPPFDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNGSDE 120
           NYPP DN S+Q  WKL+LFHRDKLPLNFDPDH RRFKERI RD +RVSSLLR+LSNGSDE
Sbjct: 61  NYPPIDN-STQKQWKLELFHRDKLPLNFDPDHRRRFKERIGRDVERVSSLLRRLSNGSDE 120

Query: 121 QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180
           QVTDFGSDV+SGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP
Sbjct: 121 QVTDFGSDVISGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDP 180

Query: 181 VFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVV 240
           VFDPA SASYAGISCDSSVC RLDNAGCN+GRCRYEVSYGDGSYTRG LALETLTFGRV+
Sbjct: 181 VFDPASSASYAGISCDSSVCGRLDNAGCNDGRCRYEVSYGDGSYTRGNLALETLTFGRVL 240

Query: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE 300
           IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE
Sbjct: 241 IRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLE 300

Query: 301 FGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT 360
           FGR AMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT
Sbjct: 301 FGRSAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDT 360

Query: 361 GTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGP 420
           GTAVTRLP PAYEA RDTFIGQTANLPRS  VSIFDTCY+LNGFVSVRVPTVSFYFSGGP
Sbjct: 361 GTAVTRLPVPAYEAFRDTFIGQTANLPRSREVSIFDTCYDLNGFVSVRVPTVSFYFSGGP 420

Query: 421 ILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           ILTLPARNFLIPVDGEGTFCFAFAAS SGLSIIGNIQQEGIQIS+DG+NGFVGFGPSIC
Sbjct: 421 ILTLPARNFLIPVDGEGTFCFAFAASPSGLSIIGNIQQEGIQISVDGANGFVGFGPSIC 478

[Close]

BLAST of Lsi09G008490 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 629.4 bits (1622), Expect = 2.3e-180
Identity = 303/438 (69.18%), Postives = 360/438 (82.19%), Query Frame = 0


Query: 48  LPQDLDLHENYPPFDNS----SSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRV 107
           L   L +    P F+N+     S S + L+L HRD+ P     +H  R   R+ RD+ RV
Sbjct: 33  LQPPLTVTATLPDFNNTHFSDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRV 92

Query: 108 SSLLRQL------SNGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGS 167
           S++LR++      S+ S  +V DFGSD+VSG +QGSGEYFVRIGVGSPPR QY+VIDSGS
Sbjct: 93  SAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGS 152

Query: 168 DIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGD 227
           D+VWVQCQPC  CY+QSDPVFDPA S SY G+SC SSVCDR++N+GC+ G CRYEV YGD
Sbjct: 153 DMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGD 212

Query: 228 GSYTRGTLALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTG 287
           GSYT+GTLALETLTF + V+RN+A+GCGH NRGMF+GAAGLLG+GGG+MSFVGQL GQTG
Sbjct: 213 GSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTG 272

Query: 288 GAFSYCLVSRGTESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPI 347
           GAF YCLVSRGT+STG+L FGR A+PVG +WVPL+RNPRAPSFYYVGL GLGVGG+R+P+
Sbjct: 273 GAFGYCLVSRGTDSTGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPL 332

Query: 348 PEQIFELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNL 407
           P+ +F+LT+ G GGVVMDTGTAVTRLP  AY A RD F  QTANLPR+  VSIFDTCY+L
Sbjct: 333 PDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDL 392

Query: 408 NGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGI 467
           +GFVSVRVPTVSFYF+ GP+LTLPARNFL+PVD  GT+CFAFAAS +GLSIIGNIQQEGI
Sbjct: 393 SGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGI 452

Query: 468 QISIDGSNGFVGFGPSIC 476
           Q+S DG+NGFVGFGP++C
Sbjct: 453 QVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Lsi09G008490 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 406.4 bits (1043), Expect = 3.2e-113
Identity = 223/500 (44.60%), Postives = 311/500 (62.20%), Query Frame = 0


Query: 4   FLLLLPL----LATTVSPVATGPAATYPATQLLNVKDTIKETETRPSRLPQDLDLHENYP 63
           FL LL +    L  T +  ++   +T P T +L+V  ++++T+T  S  P    L    P
Sbjct: 6   FLSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKP 65

Query: 64  P------FDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLS-- 123
                  F NSS  SP  L+L  RD    +   D+      R+ERDS RV+ ++ ++   
Sbjct: 66  ESLSDPVFFNSS--SPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFA 125

Query: 124 -NGSDE-------------QVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGS 183
             G D              Q  D  + VVSG  QGSGEYF RIGVG+P +  Y+V+D+GS
Sbjct: 126 VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGS 185

Query: 184 DIVWVQCQPCSECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGRCRYEVSYGD 243
           D+ W+QC+PC++CYQQSDPVF+P  S++Y  ++C +  C  L+ + C   +C Y+VSYGD
Sbjct: 186 DVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGD 245

Query: 244 GSYTRGTLALETLTFGRV-VIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQT 303
           GS+T G LA +T+TFG    I N+A+GCGH N G+F GAAGLLGLGGG +S   Q+    
Sbjct: 246 GSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---K 305

Query: 304 GGAFSYCLVSRGTESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP 363
             +FSYCLV R +  + +L+F    +  G A  PL+RN +  +FYYVGLSG  VGG +V 
Sbjct: 306 ATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVV 365

Query: 364 IPEQIFELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPR-SGRVSIFDTCY 423
           +P+ IF++   G GGV++D GTAVTRL   AY +LRD F+  T NL + S  +S+FDTCY
Sbjct: 366 LPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCY 425

Query: 424 NLNGFVSVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQE 476
           + +   +V+VPTV+F+F+GG  L LPA+N+LIPVD  GTFCFAFA ++S LSIIGN+QQ+
Sbjct: 426 DFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQ 485

[Close]

BLAST of Lsi09G008490 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 2.1e-112
Identity = 206/470 (43.83%), Postives = 297/470 (63.19%), Query Frame = 0


Query: 22  PAATYPATQLLNVKDTIKETE-TRPSRLPQDLDLHENYPPFDNSSSQSPWKLKLFHRDKL 81
           P  +   T +LNV D+I  T+ T   RL Q  +           S+ S + L+L  R  +
Sbjct: 26  PETSTTTTSILNVADSIHRTKYTSSFRLNQQEE--------QTHSASSSFSLQLHSRVSV 85

Query: 82  PLNFDPDHSRRFKERIERDSKRVSSL---------------LRQLSNGSDEQVTDFGSDV 141
                 D+      R+ RD+ RV SL               L+ +S     +  D  + +
Sbjct: 86  RGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQDIEAPL 145

Query: 142 VSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECYQQSDPVFDPAGSAS 201
           +SGT QGSGEYF R+G+G P R  Y+V+D+GSD+ W+QC PC++CY Q++P+F+P+ S+S
Sbjct: 146 ISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSS 205

Query: 202 YAGISCDSSVCDRLDNAGCNEGRCRYEVSYGDGSYTRGTLALETLTFGRVVIRNIAIGCG 261
           Y  +SCD+  C+ L+ + C    C YEVSYGDGSYT G  A ETLT G  +++N+A+GCG
Sbjct: 206 YEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCG 265

Query: 262 HMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSRGTESTGTLEFGRGAMPVG 321
           H N G+FVGAAGLLGLGGG ++   QL      +FSYCLV R ++S  T++FG    P  
Sbjct: 266 HSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCLVDRDSDSASTVDFGTSLSP-D 325

Query: 322 GAWVPLIRNPRAPSFYYVGLSGLGVGGIRVPIPEQIFELTDLGYGGVVMDTGTAVTRLPA 381
               PL+RN +  +FYY+GL+G+ VGG  + IP+  FE+ + G GG+++D+GTAVTRL  
Sbjct: 326 AVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQT 385

Query: 382 PAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSVRVPTVSFYFSGGPILTLPARNF 441
             Y +LRD+F+  T +L ++  V++FDTCYNL+   +V VPTV+F+F GG +L LPA+N+
Sbjct: 386 EIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNY 445

Query: 442 LIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDGSNGFVGFGPSIC 476
           +IPVD  GTFC AFA +AS L+IIGN+QQ+G +++ D +N  +GF  + C
Sbjct: 446 MIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

[Close]

BLAST of Lsi09G008490 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 396.7 bits (1018), Expect = 2.5e-110
Identity = 215/432 (49.77%), Postives = 274/432 (63.43%), Query Frame = 0


Query: 54  LHENYPPFDNSSSQSPWKLKLFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLLRQLSNG 113
           L   +    +S S S   L L H D L  N  PD    F  R++RDS+RV S+    +  
Sbjct: 56  LESEFESGSDSESSSSITLNLDHIDALSSNKTPD--ELFSSRLQRDSRRVKSIATLAAQI 115

Query: 114 SDEQVT------DFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPC 173
               VT       F S VVSG  QGSGEYF R+GVG+P R  Y+V+D+GSDIVW+QC PC
Sbjct: 116 PGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPC 175

Query: 174 SECYQQSDPVFDPAGSASYAGISCDSSVCDRLDNAGCNEGR--CRYEVSYGDGSYTRGTL 233
             CY QSDP+FDP  S +YA I C S  C RLD+AGCN  R  C Y+VSYGDGS+T G  
Sbjct: 176 RRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDF 235

Query: 234 ALETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLV 293
           + ETLTF R  ++ +A+GCGH N G+FVGAAGLLGLG G +SF GQ G +    FSYCLV
Sbjct: 236 STETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLV 295

Query: 294 SRGTES-TGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP-IPEQIFE 353
            R   S   ++ FG  A+     + PL+ NP+  +FYYVGL G+ VGG RVP +   +F+
Sbjct: 296 DRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFK 355

Query: 354 LTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFVSV 413
           L  +G GGV++D+GT+VTRL  PAY A+RD F      L R+   S+FDTC++L+    V
Sbjct: 356 LDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEV 415

Query: 414 RVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISIDG 473
           +VPTV  +F G  + +LPA N+LIPVD  G FCFAFA +  GLSIIGNIQQ+G ++  D 
Sbjct: 416 KVPTVVLHFRGADV-SLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDL 475

Query: 474 SNGFVGFGPSIC 476
           ++  VGF P  C
Sbjct: 476 ASSRVGFAPGGC 484

[Close]

BLAST of Lsi09G008490 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 366.3 bits (939), Expect = 3.7e-101
Identity = 200/434 (46.08%), Postives = 268/434 (61.75%), Query Frame = 0


Query: 62  DNSSSQSPWKLK--LFHRDKLPLNFDPDHSRRFKERIERDSKRVSSLL--------RQLS 121
           D S S+S   L   L H D L    D   +  F  R++RDS RV S+         R  +
Sbjct: 51  DESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNAT 110

Query: 122 NGSDEQVTDFGSDVVSGTEQGSGEYFVRIGVGSPPRSQYVVIDSGSDIVWVQCQPCSECY 181
             +      F   V+SG  QGSGEYF+R+GVG+P  + Y+V+D+GSD+VW+QC PC  CY
Sbjct: 111 KRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACY 170

Query: 182 QQSDPVFDPAGSASYAGISCDSSVCDRLDNAG-CNEGR---CRYEVSYGDGSYTRGTLAL 241
            Q+D +FDP  S ++A + C S +C RLD++  C   R   C Y+VSYGDGS+T G  + 
Sbjct: 171 NQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFST 230

Query: 242 ETLTFGRVVIRNIAIGCGHMNRGMFVGAAGLLGLGGGAMSFVGQLGGQTGGAFSYCLVSR 301
           ETLTF    + ++ +GCGH N G+FVGAAGLLGLG G +SF  Q   +  G FSYCLV R
Sbjct: 231 ETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDR 290

Query: 302 -----GTESTGTLEFGRGAMPVGGAWVPLIRNPRAPSFYYVGLSGLGVGGIRVP-IPEQI 361
                 ++   T+ FG  A+P    + PL+ NP+  +FYY+ L G+ VGG RVP + E  
Sbjct: 291 TSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQ 350

Query: 362 FELTDLGYGGVVMDTGTAVTRLPAPAYEALRDTFIGQTANLPRSGRVSIFDTCYNLNGFV 421
           F+L   G GGV++D+GT+VTRL  PAY ALRD F      L R+   S+FDTC++L+G  
Sbjct: 351 FKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMT 410

Query: 422 SVRVPTVSFYFSGGPILTLPARNFLIPVDGEGTFCFAFAASASGLSIIGNIQQEGIQISI 476
           +V+VPTV F+F GG + +LPA N+LIPV+ EG FCFAFA +   LSIIGNIQQ+G +++ 
Sbjct: 411 TVKVPTVVFHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAY 470

The following BLAST results are available for this feature:

BLAST of Lsi09G008490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHE3	3.3e-179	69.18	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LS40	4.5e-112	44.60	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	3.6e-109	49.77	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C2	3.4e-67	38.56	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	5.8e-67	37.82	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of Lsi09G008490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VGC3	5.7e-259	94.76	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CNF7	7.4e-259	94.76	protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucumis melo OX=3656 GN=LOC10350290...	[more]
A0A6J1E0R8	1.0e-247	90.63	protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JNZ5	1.3e-247	90.81	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1CII3	6.3e-242	89.05	protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of Lsi09G008490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875928.1	9.3e-264	96.42	protein ASPARTIC PROTEASE IN GUARD CELL 2 [Benincasa hispida]	[more]
KAA0066127.1	1.2e-258	94.76	protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo var. makuwa] >TYK15170.1...	[more]
XP_008465249.1	1.5e-258	94.76	PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis melo]	[more]
XP_004150193.1	1.7e-257	94.76	protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus] >KAE8648376.1 hypoth...	[more]
KAG7032671.1	4.2e-248	91.23	Protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of Lsi09G008490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20015.1	2.3e-180	69.18	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	3.2e-113	44.60	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.1e-112	43.83	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	2.5e-110	49.77	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	3.7e-101	46.08	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 306..475 e-value: 1.0E-41 score: 144.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 113..298 e-value: 1.8E-50 score: 173.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 131..475
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 320..471 e-value: 8.2E-30 score: 103.7
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 136..298 e-value: 9.7E-50 score: 169.2
None	No IPR available	PANTHER	PTHR13683:SF838	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2-LIKE	coord: 63..475
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 63..475
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 151..162
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 136..471 score: 44.99424

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G008490.1	Lsi09G008490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity