Lsi09G008040 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi09G008040 vs. ExPASy Swiss-Prot
Match: Q5MAU8 (Probable inactive purple acid phosphatase 27 OS=Arabidopsis thaliana OX=3702 GN=PAP27 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.9e-73
Identity = 167/501 (33.33%), Postives = 245/501 (48.90%), Query Frame = 0

Query: 62  LEVVSSSESLSNEEFINVTAAVLSSDPDYLPCNKKG-----CGVP-----SAGGRCAVET 121
           + VV S+   S+++++ V +          P + K      C  P     +      ++T
Sbjct: 64  VNVVISNPEPSSDDWVGVFSPAKFDSSSCAPTDDKEIAPFICSAPVKYMYAKSSPDYMKT 123

Query: 122 CNATVSFHVINFRTDVEFVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLS--------- 181
            NA + F +IN R D  F LF GG   P L+  S  ++F NP AP+Y  L+         
Sbjct: 124 GNAVLKFMLINQRADFSFALFTGGLSNPTLVSVSNHVSFINPKAPVYPRLALGKKWDEMT 183

Query: 182 ------------------SIDSTATSINEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAV 241
                             S   T +  +    +    +S   +PA+  GW DPGFIH+A 
Sbjct: 184 VTWTSGYNIGEAVPFVEWSRKGTRSRRSPAGTLTFTRNSMCGAPARTVGWRDPGFIHTAS 243

Query: 242 MTQLHPSTTYSYKYGSD----KVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFS 301
           +  L P+  Y+Y+ G +     + WS   TFK+ P  G+ +    + FGDMGK   D  +
Sbjct: 244 LKDLWPNLKYTYRMGHELMNGSIVWSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSN 303

Query: 302 VEHYIQPGSISVVQAMKEEVESGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPY 361
             +  QPGS++    + ++++  N+D +FHIGDI+YA G++ +WD F   + PIAS +PY
Sbjct: 304 EYNDYQPGSLNTTDQLIKDLK--NIDIVFHIGDITYANGYISQWDQFTAQVEPIASTVPY 363

Query: 362 MTAMGNHERDYDESGSVYTFPDSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIIS 421
           M A GNHERD+ +SGS Y   DSGGECGVP ET F  P   K + WYS +     F +  
Sbjct: 364 MVASGNHERDWPDSGSFYGGKDSGGECGVPAETMFDFPAENKAKFWYSADYGMFRFCVAD 423

Query: 422 TEHDFTINSPQYEWMKNDMASVDRSRTPWLIFTGHRPMYSSIH------GLFVAPSVDPN 481
           TEHD+   S QY++++  +ASVDR   PWLIF  HR +  S +      G F  P    +
Sbjct: 424 TEHDWREGSEQYQFIERCLASVDRRAQPWLIFIAHRVLGYSTNDWYGQEGSFEEPMGRES 483

Query: 482 FVA-----AVDLVLFGHVHNYERTCSVVNNICKGMPSKDIDGIDTYDHNNYSAPLHAVIG 511
                    VD+  +GHVHNYERTC +  N C       +D   ++    +   +H V+G
Sbjct: 484 LQKLWQKYKVDIAFYGHVHNYERTCPIYQNQC-------MDNEKSHYSGAFKGTIHVVVG 543

BLAST of Lsi09G008040 vs. ExPASy Swiss-Prot
Match: Q9LMX4 (Probable inactive purple acid phosphatase 1 OS=Arabidopsis thaliana OX=3702 GN=PAP1 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 5.6e-72
Identity = 157/446 (35.20%), Postives = 231/446 (51.79%), Query Frame = 0

Query: 111 TCNATVSFHVINFRTDVEFVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLS-------- 170
           T   ++   +IN R+D  F LF GG + P L+  S  + F+NPNAP+Y  L+        
Sbjct: 125 TGTGSLKLQLINQRSDFSFALFSGGLLNPKLVAISNKVAFENPNAPVYPRLALGKEWDEM 184

Query: 171 -------------------SIDSTATSINEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSA 230
                               +      ++    +    +S   +PA+  GW DPG+IH+A
Sbjct: 185 TVTWTSGYGLNLAEPVVEWGVKGGERKLSPAGTLTFARNSMCGAPARTVGWRDPGYIHTA 244

Query: 231 VMTQLHPSTTYSYKYG----SDKVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSF 290
            + +L P++ Y+Y+ G    +  + WS E  FK+ P  G+ +    + FGDMGKA +D  
Sbjct: 245 FLKELWPNSKYTYRVGHRLSNGALIWSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGS 304

Query: 291 SVEHYIQPGSISVVQAMKEEVESGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLP 350
           S  +  Q  S++  + + ++++    DA+FHIGDI YA G+L +WD F+  I PIAS +P
Sbjct: 305 SEYNDFQRASLNTTKQLIKDLK--KTDAVFHIGDICYANGYLSQWDQFIAQIEPIASTVP 364

Query: 351 YMTAMGNHERDYDESGSVYTFPDSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTII 410
           YM A GNHER +  SGS Y   DSGGECGVP ET F +P   + + WYS +     F + 
Sbjct: 365 YMIASGNHERVWPNSGSFYEGLDSGGECGVPAETMFYVPAQNRAKVWYSSDYGMFRFCVA 424

Query: 411 STEHDFTINSPQYEWMKNDMASVDRSRTPWLIFTGHRPM-YSSIH-----GLFVAPSVDP 470
            TEHD+   + QY ++++ +ASVDR + PWLIF  HR + YSS +     G F  P    
Sbjct: 425 DTEHDWREGTEQYNFIEHCLASVDRQKQPWLIFLAHRVLGYSSTYFYAEEGSFAEPMGRE 484

Query: 471 NFVA-----AVDLVLFGHVHNYERTCSVVNNICKGMPSKDIDGIDTYDHNNYSAPL---- 511
           +         VD+ ++GH HNYERTC V  ++C            +++ +NY APL    
Sbjct: 485 SLQKLWQKYKVDIAIYGHAHNYERTCPVYQSVC-----------TSHEKSNYKAPLNGTI 544

BLAST of Lsi09G008040 vs. ExPASy Swiss-Prot
Match: Q8H1R2 (Probable inactive purple acid phosphatase 24 OS=Arabidopsis thaliana OX=3702 GN=PAP24 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.3e-71
Identity = 162/474 (34.18%), Postives = 232/474 (48.95%), Query Frame = 0

Query: 80  TAAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFVLFGGGFITP 139
           T  + SS   Y+ CN     + S          N T+ F +IN R DV F LF  G   P
Sbjct: 105 TPYICSSPIKYMYCNSHPDYMKSG---------NVTLKFQIINQRADVSFALFSNGVQEP 164

Query: 140 CLLLKSQPLTFQNPNAPLYGLLS----------------SIDSTATSINEFDNMGV---- 199
            LL  S P+ F NP AP+Y  L+                +ID     I E+   G+    
Sbjct: 165 HLLGVSNPVAFFNPKAPVYPRLALGKNWDEMTVTWTSGYNIDEAVPFI-EWSAKGLPARR 224

Query: 200 --------IDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKVG----WSD 259
                     +S   +PA+  GW DPGF H++ + +L P+  Y Y+ G D V     WS 
Sbjct: 225 SPAGTLTFNRNSMCGNPARGVGWRDPGFFHTSFLKELWPNREYIYRLGHDLVNGSTIWSK 284

Query: 260 ETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESGNVDA 319
             TF + P  G+ +    + FGDMGK   D  +  +  QPGS++    + ++++  ++D 
Sbjct: 285 NYTFVSSPYPGQDSKQRVIIFGDMGKGERDGSNEYNDYQPGSLNTTDQVIKDLK--DIDI 344

Query: 320 IFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDSGGEC 379
           +FHIGD++Y+ G+L +WD F   + PIAS +PYM A GNHERD+ ++GS Y   DSGGEC
Sbjct: 345 VFHIGDLTYSNGYLSQWDQFTAQVQPIASTVPYMIASGNHERDWPDTGSFYAGTDSGGEC 404

Query: 380 GVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVDRSRT 439
           GVP ET F  P   + + WY  +     F +  +EHD+   + QY++++N +A+VDR   
Sbjct: 405 GVPAETMFYFPAENRAKFWYKTDYGMFRFCVADSEHDWREGTEQYKFIENCLATVDRKTQ 464

Query: 440 PWLIFTGHRPMYSSIH------GLFVAPSVDPNFVA-----AVDLVLFGHVHNYERTCSV 499
           PWLIF  HR +  S +      G F  P    +         VDL  +GHVHNYERTC +
Sbjct: 465 PWLIFIAHRVLGYSTNDWYGKEGTFEEPMGRESLQKLWQKYKVDLAFYGHVHNYERTCPI 524

Query: 500 VNNICKGMPSKDIDGIDTYDHNNYSAPLHAVIGMAGFTLDQFPTTGIESWSLCR 511
             + C         G        +   +H V+G AG  L  F +  +  WSL R
Sbjct: 525 YESQCVNNDKDHYSG-------TFKGTIHVVVGGAGSHLSPFSSL-VPKWSLVR 558

BLAST of Lsi09G008040 vs. ExPASy Swiss-Prot
Match: Q687E1 (Nucleotide pyrophosphatase/phosphodiesterase (Fragments) OS=Hordeum vulgare OX=4513 GN=npp PE=1 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 1.0e-52
Identity = 110/299 (36.79%), Postives = 160/299 (53.51%), Query Frame = 0

Query: 223 VGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVES 282
           V W+   TF+ PP  G+ +    + FGDMGKA  D  +     QPGS++    + E+++ 
Sbjct: 23  VVWAKPYTFRAPPTPGQNSLQRIIVFGDMGKAERDGSNEFANYQPGSLNTTDRLIEDLD- 82

Query: 283 GNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPD 342
            N D +FHIGD+ YA G+L +WD F   + PI+++ PYM A GNHERD+  +G  +   D
Sbjct: 83  -NYDIVFHIGDMPYANGYLSQWDQFTAQVAPISAKKPYMVASGNHERDWPNTGGFFDVKD 142

Query: 343 SGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASV 402
           SGGECGVP ET +  P   +   WY ++     F +  +EHD+   +PQY++++  +++V
Sbjct: 143 SGGECGVPAETMYYYPAENRANFWYKVDYGMFRFCVGDSEHDWREGTPQYKFIEECLSTV 202

Query: 403 DRSRTPWLIFTGHRPM-YSS-----IHGLFVAPSVDPNFVA-----AVDLVLFGHVHNYE 462
           DR   PWLIFT HR + YSS       G F  P    +         VD+  FGHVHNYE
Sbjct: 203 DRKHQPWLIFTAHRVLGYSSNSWYADQGSFEEPEGRESLQKLWQRYRVDIAYFGHVHNYE 262

Query: 463 RTCSVVNNICKGMPSKDIDGIDTYDHNNYSAPLHAVIGMAGFTLDQFPTTGIESWSLCR 511
           RTC +  + C       ++   T+     +  +  V G  G  L  + TT I  WS+ R
Sbjct: 263 RTCPLYQSQC-------VNADKTHYSGTMNGTIFVVAGGGGSHLSSY-TTAIPKWSIFR 311

BLAST of Lsi09G008040 vs. ExPASy Swiss-Prot
Match: Q9LMG7 (Probable inactive purple acid phosphatase 2 OS=Arabidopsis thaliana OX=3702 GN=PAP2 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.9e-49
Identity = 131/335 (39.10%), Postives = 176/335 (52.54%), Query Frame = 0

Query: 187 SPAKD-FGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKVGWSDETTFKTPPAGGEGNDFHF 246
           SPA    GW DPG+I   VM  L+    Y Y+ GSD  GWS+  ++       E  +   
Sbjct: 201 SPANSTIGWRDPGWIFDTVMKNLNDGVRYYYQVGSDSKGWSEIHSYIARDVTAE--ETVA 260

Query: 247 LAFGDMGKA-PLDSFSVEHYIQPGSISVVQAMKEEVES-GNVDA-IFHIGDISYATGFLV 306
             FGDMG A P  +F      Q  SIS V+ +  ++E+ G+  A I HIGDISYA G+  
Sbjct: 261 FMFGDMGCATPYTTFI---RTQDESISTVKWILRDIEALGDKPAMISHIGDISYARGYSW 320

Query: 307 EWDFFLHLINPIASRLPYMTAMGNHERDY-------DESGSVYTFPDSGGECGVPYETYF 366
            WD F   + PIAS +PY   +GNHE D+       D + S+Y   D GGECGVPY   F
Sbjct: 321 VWDEFFAQVEPIASTVPYHVCIGNHEYDFSTQPWKPDWAASIYG-NDGGGECGVPYSLKF 380

Query: 367 KMP-----ISGKDQP-----WYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVDRS 426
            MP      +G   P     +YS ++ ++HF  ISTE +F     QYE++K D+ SVDR 
Sbjct: 381 NMPGNSSESTGMKAPPTRNLYYSYDMGTVHFVYISTETNFLKGGSQYEFIKRDLESVDRK 440

Query: 427 RTPWLIFTGHRPMYSSIHGL-------FVAPSVDPNFVA-AVDLVLFGHVHNYERTCSVV 486
           +TP+++  GHRPMY++ + +        +   ++P FV   V L L+GHVH YER C + 
Sbjct: 441 KTPFVVVQGHRPMYTTSNEVRDTMIRQKMVEHLEPLFVKNNVTLALWGHVHRYERFCPIS 500

Query: 487 NNICKGMPSKDIDGIDTYDHNNYSAPLHAVIGMAG 493
           NN C            T    N   P+H VIGMAG
Sbjct: 501 NNTC-----------GTQWQGN---PVHLVIGMAG 515

BLAST of Lsi09G008040 vs. ExPASy TrEMBL
Match: A0A6J1DVP7 (Purple acid phosphatase OS=Momordica charantia OX=3673 GN=LOC111024493 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 4.1e-227
Identity = 408/605 (67.44%), Postives = 448/605 (74.05%), Query Frame = 0

Query: 3   LLFLLSFFFLSSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCPNINAFIQL 62
           LLFLL  FF SS    SSSLHPL VDSAKLHRNYTAISDFRLLNRRKLI+CPN+N FIQ+
Sbjct: 13  LLFLLPLFFPSS----SSSLHPLVVDSAKLHRNYTAISDFRLLNRRKLIDCPNLNQFIQI 72

Query: 63  EVVSSSESLSNEEFINVT------------------------------------------ 122
           EV+S+ + LSNEEFINVT                                          
Sbjct: 73  EVISNFKDLSNEEFINVTVNGVLIPLEDHWVAMITPSNANVHGCPLSEGFYLQTGDFSSL 132

Query: 123 ---------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFVL 182
                    AA L SDPDY+ C KK C   S GGRC +ETC+AT+SFHVINFRTDVEF L
Sbjct: 133 PLLCHYPVKAAFLKSDPDYMGCKKKEC-KKSVGGRCLLETCSATLSFHVINFRTDVEFAL 192

Query: 183 FGGGFITPCLLLK-SQPLTFQNPNAPLYGLLSSIDSTATS-------------------- 242
           FGGGFI PCL+LK + PL FQNPNAPLYG LSS DST TS                    
Sbjct: 193 FGGGFIAPCLILKTTPPLNFQNPNAPLYGHLSSTDSTGTSMRVSWVSGDKEPQQVQYGEE 252

Query: 243 -------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKV 302
                  ++ F    + +S+SIQSPA+DFGWHDPGFIHSAVMTQL PSTTYSY+YGSD V
Sbjct: 253 GRTQTSQVSTFSQNDMCNSASIQSPARDFGWHDPGFIHSAVMTQLQPSTTYSYRYGSDSV 312

Query: 303 GWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESG 362
           GWSD  TFKTPPA G   +F+F+AFGDMGKAPLDS SVEHYIQPGSI VV+AM+EEVESG
Sbjct: 313 GWSDRKTFKTPPAAGAAKEFNFIAFGDMGKAPLDSSSVEHYIQPGSIPVVKAMEEEVESG 372

Query: 363 NVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDS 422
            +DA+FHIGDISYATGFLVEWDFFLHLINPIASR+PYMTA+GNHERDYDESGS+Y  PDS
Sbjct: 373 EIDAVFHIGDISYATGFLVEWDFFLHLINPIASRVPYMTAIGNHERDYDESGSMYKLPDS 432

Query: 423 GGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD 482
           GGECGVPYETYF+MPIS KD+PWYSIEIAS+HFT+ISTEHDF+INSPQYEWMKNDMASVD
Sbjct: 433 GGECGVPYETYFQMPISAKDEPWYSIEIASVHFTLISTEHDFSINSPQYEWMKNDMASVD 492

Query: 483 RSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYERTC 520
           RS+TPWLIFTGHRPMYSS+ G  + PSVDP FV A         VDL LFGHVHNYERTC
Sbjct: 493 RSKTPWLIFTGHRPMYSSVDG--IIPSVDPRFVVAIEPLLLQNKVDLALFGHVHNYERTC 552

BLAST of Lsi09G008040 vs. ExPASy TrEMBL
Match: A0A6J1JQQ1 (Purple acid phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111487484 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 2.4e-222
Identity = 406/613 (66.23%), Postives = 450/613 (73.41%), Query Frame = 0

Query: 3   LLFLLSFFFLSS-SSALSSS-LHPLAVDSAKLHRNYTAISDFRLLNRRKLINCPN-INAF 62
           LLFLLSFF LSS SS+LSSS LHP   DSAKLHRNYTAISDFRLLNRRKL++CP+ IN F
Sbjct: 13  LLFLLSFFSLSSFSSSLSSSLLHPSIADSAKLHRNYTAISDFRLLNRRKLLDCPHIINPF 72

Query: 63  IQLEVVSSSESLSNEEFINVT--------------------------------------- 122
           ++L VVS SE+L NEEFINVT                                       
Sbjct: 73  VKLTVVSVSENLLNEEFINVTVSGVSIPSIEHWVAMITPSKANVDSCPLNEGLYLQTGDL 132

Query: 123 ------------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVE 182
                       AA L SDPDYL C K  C V S GGRC  +TC+AT+SFHVINFRTDVE
Sbjct: 133 SSLPLLCHYPVKAAYLRSDPDYLRCKKSECRVRS-GGRCLQKTCSATLSFHVINFRTDVE 192

Query: 183 FVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATS------------------ 242
           F LFGGGF TPCLLL+SQP  FQNPNAPLYG LS+ DST TS                  
Sbjct: 193 FALFGGGFATPCLLLRSQPRPFQNPNAPLYGFLSATDSTGTSMRLSWVSGDKNPQQLKYG 252

Query: 243 ---------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSD 302
                    ++ F    + ++ SIQSPAKDFGWHDPGFIHSAVMTQL PSTT+SYKYGSD
Sbjct: 253 QHGTTQTSQVSSFSQNDMCNTPSIQSPAKDFGWHDPGFIHSAVMTQLQPSTTFSYKYGSD 312

Query: 303 KVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVE 362
           +VGWSDE TF TPPA G+ NDFHFLAFGDMGKAPLDS SVEHYIQPGSISVV  M EEVE
Sbjct: 313 QVGWSDEATFTTPPASGDENDFHFLAFGDMGKAPLDSSSVEHYIQPGSISVVNGMTEEVE 372

Query: 363 SGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFP 422
           SG VDA+FHIGDISYATGFLVEWDFFLHLI PIASR+ YMTA+GNHERDYD+SGSVY  P
Sbjct: 373 SGKVDAVFHIGDISYATGFLVEWDFFLHLIKPIASRVAYMTAIGNHERDYDKSGSVYRLP 432

Query: 423 DSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMAS 482
           DSGGECGVPYETYF+MP  GKDQPWYSIE+AS+HFT++STEH FT  SPQYEWMK DMAS
Sbjct: 433 DSGGECGVPYETYFQMPTFGKDQPWYSIEMASVHFTVVSTEHPFTPGSPQYEWMKRDMAS 492

Query: 483 VDRSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYER 526
           V+RSRTPWL+FTGHRPMYSSI GL   PSVDPNFVAA         VDLVLFGHVHNYER
Sbjct: 493 VNRSRTPWLVFTGHRPMYSSIRGLI--PSVDPNFVAAVEPLLLQNKVDLVLFGHVHNYER 552

BLAST of Lsi09G008040 vs. ExPASy TrEMBL
Match: A0A6J1HD39 (Purple acid phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111462997 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 3.1e-222
Identity = 403/613 (65.74%), Postives = 449/613 (73.25%), Query Frame = 0

Query: 3   LLFLLSFFFL--SSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCPN-INAF 62
           LLFLLSFF L  SSSS++SS LHP   DSAKLHRNYTAISDFRLLNRRKL++CP+ IN  
Sbjct: 13  LLFLLSFFSLSSSSSSSMSSLLHPSIADSAKLHRNYTAISDFRLLNRRKLLDCPHIINPS 72

Query: 63  IQLEVVSSSESLSNEEFINVT--------------------------------------- 122
           ++L VVS+SESL NEEFINVT                                       
Sbjct: 73  VKLTVVSASESLLNEEFINVTVSGVSIPSIEHWVAMITPSKANVDSCPLNEGLYLQTGDL 132

Query: 123 ------------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVE 182
                       AA L SDPDYL C +  C V S GGRC  +TCNAT+SFHV+NFRTDVE
Sbjct: 133 SRLPLLCHYPVKAAYLRSDPDYLRCKRSECRVRS-GGRCLQKTCNATLSFHVVNFRTDVE 192

Query: 183 FVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATS------------------ 242
           F LFGGGF TPCLLL+SQP TFQNPNAPLYGLLS+ DST TS                  
Sbjct: 193 FALFGGGFATPCLLLRSQPRTFQNPNAPLYGLLSATDSTGTSMRLSWVSGDKNPQQLKYG 252

Query: 243 ---------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSD 302
                    ++ F    + ++ SIQSPAKDFGWHDPGFIHSAVMTQL PS T+SY+YGSD
Sbjct: 253 QHGTTQTSQVSSFSQNDMCNTPSIQSPAKDFGWHDPGFIHSAVMTQLQPSATFSYQYGSD 312

Query: 303 KVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVE 362
           +VGWSDE TF TPPAGG+ NDFHFLAFGDMGKAPLDS SVEHYIQPGSISVV  M EEVE
Sbjct: 313 QVGWSDEATFTTPPAGGDENDFHFLAFGDMGKAPLDSSSVEHYIQPGSISVVNGMAEEVE 372

Query: 363 SGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFP 422
           S  VDA+FHIGDISYATGFLVEWDFFLHLI PIASR+ YMTA+GNHERDYD+SGSVY  P
Sbjct: 373 SRKVDAVFHIGDISYATGFLVEWDFFLHLIKPIASRVAYMTAIGNHERDYDQSGSVYRLP 432

Query: 423 DSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMAS 482
           DSGGECGVPYETYF+MP  GKDQPWY+IE+AS+HFT++STEH FT  SPQYEWMK DMAS
Sbjct: 433 DSGGECGVPYETYFQMPTPGKDQPWYAIEMASVHFTVVSTEHPFTPGSPQYEWMKKDMAS 492

Query: 483 VDRSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYER 526
           V+RSRTPWL+FTGHRPMYSS  G    PSVDPNFVAA         VDLVLFGHVHNYER
Sbjct: 493 VNRSRTPWLVFTGHRPMYSSTRGFI--PSVDPNFVAAVEPLLLQNKVDLVLFGHVHNYER 552

BLAST of Lsi09G008040 vs. ExPASy TrEMBL
Match: A0A1S3AV65 (Purple acid phosphatase OS=Cucumis melo OX=3656 GN=LOC103483017 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 3.2e-211
Identity = 387/607 (63.76%), Postives = 431/607 (71.00%), Query Frame = 0

Query: 3   LLFLLSFFFLSSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCP-NINAFIQ 62
           LLFLL   F   S   SSSLHPL VDS KLH N TAISDFRLLNRR LI+CP N+N +I+
Sbjct: 4   LLFLLISLFFPYS---SSSLHPLVVDSEKLHLNSTAISDFRLLNRRSLISCPINLNPYIK 63

Query: 63  LEVVSSSE-SLSNEEFINVT---------------------------------------- 122
           LEV+S S+ SL +EEFINV                                         
Sbjct: 64  LEVISKSKNSLLDEEFINVVVTGVSIPSIDHWVALITPSNANVDGCPENKALYLQTGDLS 123

Query: 123 -----------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEF 182
                      A  L SDPDYL C K+ C     G  C ++TCNATVSFHVINFRTDVE 
Sbjct: 124 SLPLLCHYPVKAVYLRSDPDYLRCKKREC-KKRVGNNCVIQTCNATVSFHVINFRTDVEV 183

Query: 183 VLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATS------------------- 242
            +FGGGF +PCL L+S PL F NPNAPLYG LSS+DSTATS                   
Sbjct: 184 AMFGGGFTSPCLYLRSHPLPFLNPNAPLYGHLSSLDSTATSMRLSWVSGNKNPQQVQYGK 243

Query: 243 ---------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSD 302
                    ++ F    + D+  IQSPAKDFGWHDPGFIHSAVMTQL PSTTYSY+YGSD
Sbjct: 244 DGTIKKTSLVSTFSQNDMCDTPLIQSPAKDFGWHDPGFIHSAVMTQLQPSTTYSYRYGSD 303

Query: 303 KVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVE 362
            VGWS++TTF+TPPAGG GNDFHF+AFGDMGKAPLDS SVEHYIQPGSISVV+AMKEEVE
Sbjct: 304 SVGWSNQTTFRTPPAGGGGNDFHFIAFGDMGKAPLDSSSVEHYIQPGSISVVEAMKEEVE 363

Query: 363 SGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFP 422
            G +D +FHIGDISYATGFLVEWDFFLHLINPIASRLPYMTA+GNHERDY +SGSVY+  
Sbjct: 364 RGEIDGVFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAIGNHERDYVKSGSVYSLT 423

Query: 423 DSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMAS 482
           DSGGECGVPYETYF+MP +GKD+PWYSIE+ASIHFTIISTEH+F+INSPQYEWMK+DMAS
Sbjct: 424 DSGGECGVPYETYFQMPNNGKDKPWYSIEMASIHFTIISTEHNFSINSPQYEWMKSDMAS 483

Query: 483 VDRSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFV---------AAVDLVLFGHVHNYER 520
           V+RSRTPWLIF GHRPMYSSI GL    SVD NFV           VDL LFGHVHNYER
Sbjct: 484 VNRSRTPWLIFMGHRPMYSSIRGL--PTSVDHNFVDEVEPLLLQYKVDLALFGHVHNYER 543

BLAST of Lsi09G008040 vs. ExPASy TrEMBL
Match: A0A0A0KRS7 (Purple acid phosphatase OS=Cucumis sativus OX=3659 GN=Csa_5G441650 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 6.0e-210
Identity = 379/593 (63.91%), Postives = 428/593 (72.18%), Query Frame = 0

Query: 3   LLFLLSFFFLSSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCP-NINAFIQ 62
           LLFL+   F    S+ SSSLHPL VDS K H N TAISDFRLLNRR LI+CP N+N +I+
Sbjct: 4   LLFLVISLFFPFYSSSSSSLHPLVVDSEKFHLNSTAISDFRLLNRRSLISCPINLNPYIK 63

Query: 63  LEVVS-SSESLSNEEFINVTAA-------------------------------------- 122
           LEV+S S + L +EEF++V  +                                      
Sbjct: 64  LEVISKSKDGLLDEEFLDVVVSGVSIPSIDHWVALITPSNANVDGCPESKALYLQTGDLI 123

Query: 123 VLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFVLFGGGFITPCLL 182
            L SDPDYL C K+ C     G  C ++TCNATVSFHVINFRTDVE  LFGGGF +PCL 
Sbjct: 124 YLRSDPDYLQCKKREC-KKRVGNNCVIQTCNATVSFHVINFRTDVEVALFGGGFTSPCLY 183

Query: 183 LKSQPLTFQNPNAPLYGLLSSIDSTATS---------------------------INEFD 242
           L+SQPL F NP+APLYG LSS+DSTATS                           ++ F 
Sbjct: 184 LRSQPLPFLNPSAPLYGQLSSLDSTATSMRLSWVSGDQNPQQVQYGKDGTRKTSIVSTFS 243

Query: 243 NMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKVGWSDETTFKTPP 302
              + ++S IQSPAKDFGWHDPGFIHSAVMTQL PSTTYSY YGSD VGWS++TTF+TPP
Sbjct: 244 QNDMCNTSRIQSPAKDFGWHDPGFIHSAVMTQLQPSTTYSYTYGSDSVGWSNQTTFRTPP 303

Query: 303 AGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESGNVDAIFHIGDIS 362
           AGG GNDFHF+AFGDMGKAPLDS S EHYIQPGSISVV+AMKEEVE G +D +FHIGDIS
Sbjct: 304 AGGGGNDFHFIAFGDMGKAPLDSSSAEHYIQPGSISVVEAMKEEVERGEIDGVFHIGDIS 363

Query: 363 YATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDSGGECGVPYETYF 422
           YATGFLVEWDFFLHLINPIASRLPYMTA+GNHERDY +SGSVY+  DSGGECGVPYETYF
Sbjct: 364 YATGFLVEWDFFLHLINPIASRLPYMTAIGNHERDYLKSGSVYSLTDSGGECGVPYETYF 423

Query: 423 KMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVDRSRTPWLIFTGH 482
           +MP  GKD+PWYSIE+ASIHFTIISTEH+F+INSPQYEWMK+DMASV+RSRTPWLIF GH
Sbjct: 424 QMPNYGKDKPWYSIEMASIHFTIISTEHNFSINSPQYEWMKSDMASVNRSRTPWLIFMGH 483

Query: 483 RPMYSSIHGLFVAPSVDPNFV---------AAVDLVLFGHVHNYERTCSVVNNICKGMPS 520
           RPMYSSI    + PSVDP FV           VDL LFGHVHNYERTCSV  + CK MP 
Sbjct: 484 RPMYSSIRS--IPPSVDPYFVDEVEPLLLQYQVDLALFGHVHNYERTCSVFEDNCKAMPF 543

BLAST of Lsi09G008040 vs. NCBI nr
Match: XP_038875449.1 (probable inactive purple acid phosphatase 27 [Benincasa hispida])

HSP 1 Score: 897.1 bits (2317), Expect = 7.9e-257
Identity = 456/611 (74.63%), Postives = 483/611 (79.05%), Query Frame = 0

Query: 3   LLFLLSFFFLS-SSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCPNINAFIQ 62
           LLFLL FF LS SSS+ SSSLHPLA +SAKLH+NYTAISDFRLLNRRKLINCPNIN+FIQ
Sbjct: 13  LLFLLPFFLLSWSSSSSSSSLHPLAAESAKLHQNYTAISDFRLLNRRKLINCPNINSFIQ 72

Query: 63  LEVVSSSESLSNEEFINVT----------------------------------------- 122
           LEV+S+SE+L NEEFINVT                                         
Sbjct: 73  LEVISNSETLLNEEFINVTVSGVLFPSEDHWIAMITPSNANVDDCPLSKFLYVQTGDLSN 132

Query: 123 ----------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFV 182
                     AA LSSDPDYLPCNKK C +   GGRCA+ETC+AT+SFHVINFRTDVEFV
Sbjct: 133 LPLLCHYPVKAASLSSDPDYLPCNKKECKLRGPGGRCAIETCSATLSFHVINFRTDVEFV 192

Query: 183 LFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATS-------------------- 242
           LFGGGF TPCLLL+SQPL+FQNPNAPLYGLLSSIDSTATS                    
Sbjct: 193 LFGGGFFTPCLLLRSQPLSFQNPNAPLYGLLSSIDSTATSMKLSWVSGDEEPQQVQYGKD 252

Query: 243 -------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKV 302
                  ++ F    + +SSS QSPAKDFGWHDPGFIHSAVMTQL PSTTYSYKYGSDKV
Sbjct: 253 GTLQTSQVSTFSQNDMCNSSSFQSPAKDFGWHDPGFIHSAVMTQLQPSTTYSYKYGSDKV 312

Query: 303 GWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESG 362
           GWSDET F+TPPAGG+GNDFHFLAFGDMGKAPLD  SVEHYIQ GSISVVQ MKEEVESG
Sbjct: 313 GWSDETAFRTPPAGGDGNDFHFLAFGDMGKAPLDPSSVEHYIQAGSISVVQGMKEEVESG 372

Query: 363 NVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDS 422
            VDA+FHIGDISYATGFLVEWDFFLHLINPIASRLPYMTA+GNHERDY ESGSVY+ PDS
Sbjct: 373 KVDAVFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAIGNHERDYSESGSVYSLPDS 432

Query: 423 GGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD 482
           GGECGVPYETYF+MPIS KDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD
Sbjct: 433 GGECGVPYETYFQMPISAKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD 492

Query: 483 RSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYERTC 526
           RSRTPWLIFTGHRPMYSSIHGLF  PSVDPNFVAA         VDLVLFGHVHNYERTC
Sbjct: 493 RSRTPWLIFTGHRPMYSSIHGLF--PSVDPNFVAAVEPLLLQNKVDLVLFGHVHNYERTC 552

BLAST of Lsi09G008040 vs. NCBI nr
Match: XP_031740989.1 (probable inactive purple acid phosphatase 27 isoform X1 [Cucumis sativus])

HSP 1 Score: 803.1 bits (2073), Expect = 1.6e-228
Identity = 411/611 (67.27%), Postives = 452/611 (73.98%), Query Frame = 0

Query: 3   LLFLLSFFFLSSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCP-NINAFIQ 62
           LL LL F  LSS    SS+LHPLA  SA LHRNYTAISDFRLLNRR   +CP N N FI 
Sbjct: 16  LLSLLQFSLLSS----SSTLHPLAAHSANLHRNYTAISDFRLLNRRTFFSCPTNRNFFIN 75

Query: 63  LEVVSSSESLSNEEFINVT----------------------------------------- 122
           ++V+S S SL NEEF+NVT                                         
Sbjct: 76  IDVISKSNSLLNEEFVNVTVGGITNPSKDHWIAMITPSNANVEDCSVSSILYGQTGDLTL 135

Query: 123 ----------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFV 182
                     AA LSSDPDYLPC KKGC VP  G +C   TC AT+SFH+INFRTDVEF 
Sbjct: 136 LPLLCHYPVKAAYLSSDPDYLPCKKKGCVVPPVGDKCEEPTCIATLSFHIINFRTDVEFF 195

Query: 183 LFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATSIN-------------EFDNM 242
           LF GGF+TPCLL KS+ L+FQNPNAPLYG LSSIDSTATS+              ++D  
Sbjct: 196 LFDGGFLTPCLLYKSKTLSFQNPNAPLYGHLSSIDSTATSMRLSWVSGDGEPQQVQYDED 255

Query: 243 GVIDSSSI--------------QSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKV 302
           G I +S +              QSPAKDFGWHDPGFIH+A+MTQL PSTTYSYKYGS+KV
Sbjct: 256 GKIQTSQVSTFSQNDMCNASFLQSPAKDFGWHDPGFIHTAIMTQLKPSTTYSYKYGSEKV 315

Query: 303 GWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESG 362
           GWS+ETTF+TPPA G+  DF F+AFGDMGKAPLDS S EHYIQPGSISVV+AMKEEVE G
Sbjct: 316 GWSEETTFRTPPAAGDETDFSFIAFGDMGKAPLDSSSAEHYIQPGSISVVEAMKEEVERG 375

Query: 363 NVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDS 422
            +D +FHIGDISYATGFLVEWDFFLHLINPIASRLPYMTA+GNHERDY +S SVYTFPDS
Sbjct: 376 EIDGVFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAIGNHERDYLQSTSVYTFPDS 435

Query: 423 GGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD 482
           GGECGVPYETY +MPISGKDQPWYSIE+ASIHFTIISTEHDFTINSPQYEWMKNDMASVD
Sbjct: 436 GGECGVPYETYLQMPISGKDQPWYSIEMASIHFTIISTEHDFTINSPQYEWMKNDMASVD 495

Query: 483 RSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYERTC 526
           RSRTPWLIF GHRPMYSSI G  + PSVDP+FVAA         VDLVLFGHVH+YERTC
Sbjct: 496 RSRTPWLIFAGHRPMYSSISGSLLIPSVDPSFVAAVEPLLLQNKVDLVLFGHVHSYERTC 555

BLAST of Lsi09G008040 vs. NCBI nr
Match: XP_031740990.1 (probable inactive purple acid phosphatase 27 isoform X2 [Cucumis sativus])

HSP 1 Score: 803.1 bits (2073), Expect = 1.6e-228
Identity = 411/611 (67.27%), Postives = 452/611 (73.98%), Query Frame = 0

Query: 3   LLFLLSFFFLSSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCP-NINAFIQ 62
           LL LL F  LSS    SS+LHPLA  SA LHRNYTAISDFRLLNRR   +CP N N FI 
Sbjct: 16  LLSLLQFSLLSS----SSTLHPLAAHSANLHRNYTAISDFRLLNRRTFFSCPTNRNFFIN 75

Query: 63  LEVVSSSESLSNEEFINVT----------------------------------------- 122
           ++V+S S SL NEEF+NVT                                         
Sbjct: 76  IDVISKSNSLLNEEFVNVTVGGITNPSKDHWIAMITPSNANVEDCSVSSILYGQTGDLTL 135

Query: 123 ----------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFV 182
                     AA LSSDPDYLPC KKGC VP  G +C   TC AT+SFH+INFRTDVEF 
Sbjct: 136 LPLLCHYPVKAAYLSSDPDYLPCKKKGCVVPPVGDKCEEPTCIATLSFHIINFRTDVEFF 195

Query: 183 LFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATSIN-------------EFDNM 242
           LF GGF+TPCLL KS+ L+FQNPNAPLYG LSSIDSTATS+              ++D  
Sbjct: 196 LFDGGFLTPCLLYKSKTLSFQNPNAPLYGHLSSIDSTATSMRLSWVSGDGEPQQVQYDED 255

Query: 243 GVIDSSSI--------------QSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKV 302
           G I +S +              QSPAKDFGWHDPGFIH+A+MTQL PSTTYSYKYGS+KV
Sbjct: 256 GKIQTSQVSTFSQNDMCNASFLQSPAKDFGWHDPGFIHTAIMTQLKPSTTYSYKYGSEKV 315

Query: 303 GWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESG 362
           GWS+ETTF+TPPA G+  DF F+AFGDMGKAPLDS S EHYIQPGSISVV+AMKEEVE G
Sbjct: 316 GWSEETTFRTPPAAGDETDFSFIAFGDMGKAPLDSSSAEHYIQPGSISVVEAMKEEVERG 375

Query: 363 NVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDS 422
            +D +FHIGDISYATGFLVEWDFFLHLINPIASRLPYMTA+GNHERDY +S SVYTFPDS
Sbjct: 376 EIDGVFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAIGNHERDYLQSTSVYTFPDS 435

Query: 423 GGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD 482
           GGECGVPYETY +MPISGKDQPWYSIE+ASIHFTIISTEHDFTINSPQYEWMKNDMASVD
Sbjct: 436 GGECGVPYETYLQMPISGKDQPWYSIEMASIHFTIISTEHDFTINSPQYEWMKNDMASVD 495

Query: 483 RSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYERTC 526
           RSRTPWLIF GHRPMYSSI G  + PSVDP+FVAA         VDLVLFGHVH+YERTC
Sbjct: 496 RSRTPWLIFAGHRPMYSSISGSLLIPSVDPSFVAAVEPLLLQNKVDLVLFGHVHSYERTC 555

BLAST of Lsi09G008040 vs. NCBI nr
Match: XP_022157882.1 (nucleotide pyrophosphatase/phosphodiesterase-like [Momordica charantia])

HSP 1 Score: 797.3 bits (2058), Expect = 8.6e-227
Identity = 408/605 (67.44%), Postives = 448/605 (74.05%), Query Frame = 0

Query: 3   LLFLLSFFFLSSSSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCPNINAFIQL 62
           LLFLL  FF SS    SSSLHPL VDSAKLHRNYTAISDFRLLNRRKLI+CPN+N FIQ+
Sbjct: 13  LLFLLPLFFPSS----SSSLHPLVVDSAKLHRNYTAISDFRLLNRRKLIDCPNLNQFIQI 72

Query: 63  EVVSSSESLSNEEFINVT------------------------------------------ 122
           EV+S+ + LSNEEFINVT                                          
Sbjct: 73  EVISNFKDLSNEEFINVTVNGVLIPLEDHWVAMITPSNANVHGCPLSEGFYLQTGDFSSL 132

Query: 123 ---------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFVL 182
                    AA L SDPDY+ C KK C   S GGRC +ETC+AT+SFHVINFRTDVEF L
Sbjct: 133 PLLCHYPVKAAFLKSDPDYMGCKKKEC-KKSVGGRCLLETCSATLSFHVINFRTDVEFAL 192

Query: 183 FGGGFITPCLLLK-SQPLTFQNPNAPLYGLLSSIDSTATS-------------------- 242
           FGGGFI PCL+LK + PL FQNPNAPLYG LSS DST TS                    
Sbjct: 193 FGGGFIAPCLILKTTPPLNFQNPNAPLYGHLSSTDSTGTSMRVSWVSGDKEPQQVQYGEE 252

Query: 243 -------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKV 302
                  ++ F    + +S+SIQSPA+DFGWHDPGFIHSAVMTQL PSTTYSY+YGSD V
Sbjct: 253 GRTQTSQVSTFSQNDMCNSASIQSPARDFGWHDPGFIHSAVMTQLQPSTTYSYRYGSDSV 312

Query: 303 GWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESG 362
           GWSD  TFKTPPA G   +F+F+AFGDMGKAPLDS SVEHYIQPGSI VV+AM+EEVESG
Sbjct: 313 GWSDRKTFKTPPAAGAAKEFNFIAFGDMGKAPLDSSSVEHYIQPGSIPVVKAMEEEVESG 372

Query: 363 NVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDS 422
            +DA+FHIGDISYATGFLVEWDFFLHLINPIASR+PYMTA+GNHERDYDESGS+Y  PDS
Sbjct: 373 EIDAVFHIGDISYATGFLVEWDFFLHLINPIASRVPYMTAIGNHERDYDESGSMYKLPDS 432

Query: 423 GGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVD 482
           GGECGVPYETYF+MPIS KD+PWYSIEIAS+HFT+ISTEHDF+INSPQYEWMKNDMASVD
Sbjct: 433 GGECGVPYETYFQMPISAKDEPWYSIEIASVHFTLISTEHDFSINSPQYEWMKNDMASVD 492

Query: 483 RSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYERTC 520
           RS+TPWLIFTGHRPMYSS+ G  + PSVDP FV A         VDL LFGHVHNYERTC
Sbjct: 493 RSKTPWLIFTGHRPMYSSVDG--IIPSVDPRFVVAIEPLLLQNKVDLALFGHVHNYERTC 552

BLAST of Lsi09G008040 vs. NCBI nr
Match: XP_023529683.1 (probable inactive purple acid phosphatase 27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786.2 bits (2029), Expect = 2.0e-223
Identity = 407/613 (66.39%), Postives = 448/613 (73.08%), Query Frame = 0

Query: 3   LLFLLSFFFLSS--SSALSSSLHPLAVDSAKLHRNYTAISDFRLLNRRKLINCPN-INAF 62
           LLFLLSFF LSS  SS+ SS LHP   DSAKLHRNYTAISDFRLLNRRKL+NCP  IN  
Sbjct: 13  LLFLLSFFSLSSPLSSSTSSLLHPSIADSAKLHRNYTAISDFRLLNRRKLLNCPRIINPS 72

Query: 63  IQLEVVSSSESLSNEEFINVT--------------------------------------- 122
           ++L +VS+SESL NEEFINVT                                       
Sbjct: 73  VKLTIVSASESLLNEEFINVTVSGVSIPSIEHWVAMITPSNSNVDSCPLNEGLYLQTGDL 132

Query: 123 ------------AAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVE 182
                       AA L SDPDYL C K  C V S GGRC  +TCNAT+SFHVINFRTDVE
Sbjct: 133 SSLPLLCHYPVKAAYLRSDPDYLRCKKSECRVRS-GGRCLQKTCNATLSFHVINFRTDVE 192

Query: 183 FVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLSSIDSTATS------------------ 242
           F LFGGGF TPCLLL+SQP TFQNPNAPLYGLLS+ DST TS                  
Sbjct: 193 FALFGGGFATPCLLLRSQPRTFQNPNAPLYGLLSATDSTGTSMRLSWVSGDKNPQQLKYG 252

Query: 243 ---------INEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSD 302
                    ++ F    + ++ SIQSPAKDFGWHDPGFIHSAVMTQL PSTT+SYK+GSD
Sbjct: 253 QHGTTQTSHVSSFSQNDMCNTPSIQSPAKDFGWHDPGFIHSAVMTQLQPSTTFSYKFGSD 312

Query: 303 KVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVE 362
           +VGWSDE TF TPPAGG+  DFHFLAFGDMGKAPLDS SVEHYIQPGSISVV  M EEVE
Sbjct: 313 QVGWSDEATFTTPPAGGDEKDFHFLAFGDMGKAPLDSSSVEHYIQPGSISVVNGMTEEVE 372

Query: 363 SGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFP 422
           SG VDA+FHIGDISYATGFLVEWDFFLHLI PIASR+PYMTA+GNHERDYD+SGSVY  P
Sbjct: 373 SGKVDAVFHIGDISYATGFLVEWDFFLHLIKPIASRVPYMTAIGNHERDYDQSGSVYRLP 432

Query: 423 DSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMAS 482
           DSGGECGVPYETYF+MP  GKDQPWYSIE+AS+HFT++STEH FT  SPQYEWMK DMAS
Sbjct: 433 DSGGECGVPYETYFQMPTFGKDQPWYSIEMASVHFTVVSTEHPFTPGSPQYEWMKKDMAS 492

Query: 483 VDRSRTPWLIFTGHRPMYSSIHGLFVAPSVDPNFVAA---------VDLVLFGHVHNYER 526
           V+RSRTPWL+FTGHRPMYSSI      PSVDPNFVAA         VDLVLFGHVHNYER
Sbjct: 493 VNRSRTPWLVFTGHRPMYSSIREFI--PSVDPNFVAAVEPLLLQNKVDLVLFGHVHNYER 552

BLAST of Lsi09G008040 vs. TAIR 10
Match: AT5G50400.1 (purple acid phosphatase 27 )

HSP 1 Score: 277.3 bits (708), Expect = 2.8e-74
Identity = 167/501 (33.33%), Postives = 245/501 (48.90%), Query Frame = 0

Query: 62  LEVVSSSESLSNEEFINVTAAVLSSDPDYLPCNKKG-----CGVP-----SAGGRCAVET 121
           + VV S+   S+++++ V +          P + K      C  P     +      ++T
Sbjct: 64  VNVVISNPEPSSDDWVGVFSPAKFDSSSCAPTDDKEIAPFICSAPVKYMYAKSSPDYMKT 123

Query: 122 CNATVSFHVINFRTDVEFVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLS--------- 181
            NA + F +IN R D  F LF GG   P L+  S  ++F NP AP+Y  L+         
Sbjct: 124 GNAVLKFMLINQRADFSFALFTGGLSNPTLVSVSNHVSFINPKAPVYPRLALGKKWDEMT 183

Query: 182 ------------------SIDSTATSINEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSAV 241
                             S   T +  +    +    +S   +PA+  GW DPGFIH+A 
Sbjct: 184 VTWTSGYNIGEAVPFVEWSRKGTRSRRSPAGTLTFTRNSMCGAPARTVGWRDPGFIHTAS 243

Query: 242 MTQLHPSTTYSYKYGSD----KVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFS 301
           +  L P+  Y+Y+ G +     + WS   TFK+ P  G+ +    + FGDMGK   D  +
Sbjct: 244 LKDLWPNLKYTYRMGHELMNGSIVWSKNFTFKSSPYPGQDSLQRVIIFGDMGKGERDGSN 303

Query: 302 VEHYIQPGSISVVQAMKEEVESGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLPY 361
             +  QPGS++    + ++++  N+D +FHIGDI+YA G++ +WD F   + PIAS +PY
Sbjct: 304 EYNDYQPGSLNTTDQLIKDLK--NIDIVFHIGDITYANGYISQWDQFTAQVEPIASTVPY 363

Query: 362 MTAMGNHERDYDESGSVYTFPDSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTIIS 421
           M A GNHERD+ +SGS Y   DSGGECGVP ET F  P   K + WYS +     F +  
Sbjct: 364 MVASGNHERDWPDSGSFYGGKDSGGECGVPAETMFDFPAENKAKFWYSADYGMFRFCVAD 423

Query: 422 TEHDFTINSPQYEWMKNDMASVDRSRTPWLIFTGHRPMYSSIH------GLFVAPSVDPN 481
           TEHD+   S QY++++  +ASVDR   PWLIF  HR +  S +      G F  P    +
Sbjct: 424 TEHDWREGSEQYQFIERCLASVDRRAQPWLIFIAHRVLGYSTNDWYGQEGSFEEPMGRES 483

Query: 482 FVA-----AVDLVLFGHVHNYERTCSVVNNICKGMPSKDIDGIDTYDHNNYSAPLHAVIG 511
                    VD+  +GHVHNYERTC +  N C       +D   ++    +   +H V+G
Sbjct: 484 LQKLWQKYKVDIAFYGHVHNYERTCPIYQNQC-------MDNEKSHYSGAFKGTIHVVVG 543

BLAST of Lsi09G008040 vs. TAIR 10
Match: AT1G13750.1 (Purple acid phosphatases superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 4.0e-73
Identity = 157/446 (35.20%), Postives = 231/446 (51.79%), Query Frame = 0

Query: 111 TCNATVSFHVINFRTDVEFVLFGGGFITPCLLLKSQPLTFQNPNAPLYGLLS-------- 170
           T   ++   +IN R+D  F LF GG + P L+  S  + F+NPNAP+Y  L+        
Sbjct: 125 TGTGSLKLQLINQRSDFSFALFSGGLLNPKLVAISNKVAFENPNAPVYPRLALGKEWDEM 184

Query: 171 -------------------SIDSTATSINEFDNMGVIDSSSIQSPAKDFGWHDPGFIHSA 230
                               +      ++    +    +S   +PA+  GW DPG+IH+A
Sbjct: 185 TVTWTSGYGLNLAEPVVEWGVKGGERKLSPAGTLTFARNSMCGAPARTVGWRDPGYIHTA 244

Query: 231 VMTQLHPSTTYSYKYG----SDKVGWSDETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSF 290
            + +L P++ Y+Y+ G    +  + WS E  FK+ P  G+ +    + FGDMGKA +D  
Sbjct: 245 FLKELWPNSKYTYRVGHRLSNGALIWSKEYQFKSSPFPGQNSVQQVVIFGDMGKAEVDGS 304

Query: 291 SVEHYIQPGSISVVQAMKEEVESGNVDAIFHIGDISYATGFLVEWDFFLHLINPIASRLP 350
           S  +  Q  S++  + + ++++    DA+FHIGDI YA G+L +WD F+  I PIAS +P
Sbjct: 305 SEYNDFQRASLNTTKQLIKDLK--KTDAVFHIGDICYANGYLSQWDQFIAQIEPIASTVP 364

Query: 351 YMTAMGNHERDYDESGSVYTFPDSGGECGVPYETYFKMPISGKDQPWYSIEIASIHFTII 410
           YM A GNHER +  SGS Y   DSGGECGVP ET F +P   + + WYS +     F + 
Sbjct: 365 YMIASGNHERVWPNSGSFYEGLDSGGECGVPAETMFYVPAQNRAKVWYSSDYGMFRFCVA 424

Query: 411 STEHDFTINSPQYEWMKNDMASVDRSRTPWLIFTGHRPM-YSSIH-----GLFVAPSVDP 470
            TEHD+   + QY ++++ +ASVDR + PWLIF  HR + YSS +     G F  P    
Sbjct: 425 DTEHDWREGTEQYNFIEHCLASVDRQKQPWLIFLAHRVLGYSSTYFYAEEGSFAEPMGRE 484

Query: 471 NFVA-----AVDLVLFGHVHNYERTCSVVNNICKGMPSKDIDGIDTYDHNNYSAPL---- 511
           +         VD+ ++GH HNYERTC V  ++C            +++ +NY APL    
Sbjct: 485 SLQKLWQKYKVDIAIYGHAHNYERTCPVYQSVC-----------TSHEKSNYKAPLNGTI 544

BLAST of Lsi09G008040 vs. TAIR 10
Match: AT4G24890.1 (purple acid phosphatase 24 )

HSP 1 Score: 272.3 bits (695), Expect = 8.9e-73
Identity = 162/474 (34.18%), Postives = 232/474 (48.95%), Query Frame = 0

Query: 80  TAAVLSSDPDYLPCNKKGCGVPSAGGRCAVETCNATVSFHVINFRTDVEFVLFGGGFITP 139
           T  + SS   Y+ CN     + S          N T+ F +IN R DV F LF  G   P
Sbjct: 105 TPYICSSPIKYMYCNSHPDYMKSG---------NVTLKFQIINQRADVSFALFSNGVQEP 164

Query: 140 CLLLKSQPLTFQNPNAPLYGLLS----------------SIDSTATSINEFDNMGV---- 199
            LL  S P+ F NP AP+Y  L+                +ID     I E+   G+    
Sbjct: 165 HLLGVSNPVAFFNPKAPVYPRLALGKNWDEMTVTWTSGYNIDEAVPFI-EWSAKGLPARR 224

Query: 200 --------IDSSSIQSPAKDFGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKVG----WSD 259
                     +S   +PA+  GW DPGF H++ + +L P+  Y Y+ G D V     WS 
Sbjct: 225 SPAGTLTFNRNSMCGNPARGVGWRDPGFFHTSFLKELWPNREYIYRLGHDLVNGSTIWSK 284

Query: 260 ETTFKTPPAGGEGNDFHFLAFGDMGKAPLDSFSVEHYIQPGSISVVQAMKEEVESGNVDA 319
             TF + P  G+ +    + FGDMGK   D  +  +  QPGS++    + ++++  ++D 
Sbjct: 285 NYTFVSSPYPGQDSKQRVIIFGDMGKGERDGSNEYNDYQPGSLNTTDQVIKDLK--DIDI 344

Query: 320 IFHIGDISYATGFLVEWDFFLHLINPIASRLPYMTAMGNHERDYDESGSVYTFPDSGGEC 379
           +FHIGD++Y+ G+L +WD F   + PIAS +PYM A GNHERD+ ++GS Y   DSGGEC
Sbjct: 345 VFHIGDLTYSNGYLSQWDQFTAQVQPIASTVPYMIASGNHERDWPDTGSFYAGTDSGGEC 404

Query: 380 GVPYETYFKMPISGKDQPWYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVDRSRT 439
           GVP ET F  P   + + WY  +     F +  +EHD+   + QY++++N +A+VDR   
Sbjct: 405 GVPAETMFYFPAENRAKFWYKTDYGMFRFCVADSEHDWREGTEQYKFIENCLATVDRKTQ 464

Query: 440 PWLIFTGHRPMYSSIH------GLFVAPSVDPNFVA-----AVDLVLFGHVHNYERTCSV 499
           PWLIF  HR +  S +      G F  P    +         VDL  +GHVHNYERTC +
Sbjct: 465 PWLIFIAHRVLGYSTNDWYGKEGTFEEPMGRESLQKLWQKYKVDLAFYGHVHNYERTCPI 524

Query: 500 VNNICKGMPSKDIDGIDTYDHNNYSAPLHAVIGMAGFTLDQFPTTGIESWSLCR 511
             + C         G        +   +H V+G AG  L  F +  +  WSL R
Sbjct: 525 YESQCVNNDKDHYSG-------TFKGTIHVVVGGAGSHLSPFSSL-VPKWSLVR 558

BLAST of Lsi09G008040 vs. TAIR 10
Match: AT1G13900.1 (Purple acid phosphatases superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.8e-50
Identity = 131/335 (39.10%), Postives = 176/335 (52.54%), Query Frame = 0

Query: 187 SPAKD-FGWHDPGFIHSAVMTQLHPSTTYSYKYGSDKVGWSDETTFKTPPAGGEGNDFHF 246
           SPA    GW DPG+I   VM  L+    Y Y+ GSD  GWS+  ++       E  +   
Sbjct: 201 SPANSTIGWRDPGWIFDTVMKNLNDGVRYYYQVGSDSKGWSEIHSYIARDVTAE--ETVA 260

Query: 247 LAFGDMGKA-PLDSFSVEHYIQPGSISVVQAMKEEVES-GNVDA-IFHIGDISYATGFLV 306
             FGDMG A P  +F      Q  SIS V+ +  ++E+ G+  A I HIGDISYA G+  
Sbjct: 261 FMFGDMGCATPYTTFI---RTQDESISTVKWILRDIEALGDKPAMISHIGDISYARGYSW 320

Query: 307 EWDFFLHLINPIASRLPYMTAMGNHERDY-------DESGSVYTFPDSGGECGVPYETYF 366
            WD F   + PIAS +PY   +GNHE D+       D + S+Y   D GGECGVPY   F
Sbjct: 321 VWDEFFAQVEPIASTVPYHVCIGNHEYDFSTQPWKPDWAASIYG-NDGGGECGVPYSLKF 380

Query: 367 KMP-----ISGKDQP-----WYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVDRS 426
            MP      +G   P     +YS ++ ++HF  ISTE +F     QYE++K D+ SVDR 
Sbjct: 381 NMPGNSSESTGMKAPPTRNLYYSYDMGTVHFVYISTETNFLKGGSQYEFIKRDLESVDRK 440

Query: 427 RTPWLIFTGHRPMYSSIHGL-------FVAPSVDPNFVA-AVDLVLFGHVHNYERTCSVV 486
           +TP+++  GHRPMY++ + +        +   ++P FV   V L L+GHVH YER C + 
Sbjct: 441 KTPFVVVQGHRPMYTTSNEVRDTMIRQKMVEHLEPLFVKNNVTLALWGHVHRYERFCPIS 500

Query: 487 NNICKGMPSKDIDGIDTYDHNNYSAPLHAVIGMAG 493
           NN C            T    N   P+H VIGMAG
Sbjct: 501 NNTC-----------GTQWQGN---PVHLVIGMAG 515

BLAST of Lsi09G008040 vs. TAIR 10
Match: AT2G03450.1 (purple acid phosphatase 9 )

HSP 1 Score: 193.0 bits (489), Expect = 6.9e-49
Identity = 124/330 (37.58%), Postives = 172/330 (52.12%), Query Frame = 0

Query: 193 GWHDPGFIHSAVMTQLHPSTTYSYKYGSDKVGWSDETTFKTPPAGGEGNDFHFLAFGDMG 252
           GW DPG+   AVM  L     Y Y+ GSD  GWS+  +F +   G E  +     FGDMG
Sbjct: 206 GWRDPGWTFDAVMKNLKQGIRYYYQVGSDLKGWSEIHSFVSRNEGSE--ETLAFMFGDMG 265

Query: 253 -KAPLDSFSVEHYIQPGSISVVQAMKEEVESGNVD---AIFHIGDISYATGFLVEWDFFL 312
              P  +F      +  S+S V+ +  ++E+   D    + HIGDISYA G+   WD F 
Sbjct: 266 CYTPYTTFIRG---EEESLSTVKWILRDIEALGDDKPVIVSHIGDISYARGYSWIWDEFF 325

Query: 313 HLINPIASRLPYMTAMGNHERDY-------DESGSVYTFPDSGGECGVPYETYFKMPISG 372
             I PIAS++PY   +GNHE D+       D +  VY   DSGGECGVPY   F MP + 
Sbjct: 326 TQIEPIASKVPYHVCIGNHEYDWPNQPWKPDWAAYVYG-KDSGGECGVPYSVKFNMPGNS 385

Query: 373 KDQP-----------WYSIEIASIHFTIISTEHDFTINSPQYEWMKNDMASVDRSRTPWL 432
            +             +YS ++ S+HF  ISTE DF     QY ++K+D+ SV+RS+TP++
Sbjct: 386 TEATGMVKGPQSRNLYYSYDMGSVHFVYISTETDFLKGGKQYSFLKSDLESVNRSKTPFV 445

Query: 433 IFTGHRPMYSSIHGLFVA-------PSVDPNFVA-AVDLVLFGHVHNYERTCSVVNNICK 492
           +  GHRPMY++   +  A         ++P  V   V + L+GHVH YER C++ NN C 
Sbjct: 446 VVQGHRPMYTTSRKIRDAAIREKMIEHLEPLLVKNNVTVALWGHVHRYERFCAISNNTCG 505