Lsi09G007640 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G007640
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Chitinase family protein
Location	chr09: 8733104 .. 8733571 (+)
RNA-Seq Expression	Lsi09G007640
Synteny	Lsi09G007640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATCCAAAATAATCTTGTTCCTCTCCTTCATCTTCTTCTTTGGAATGGGCCTCCCGTCGATGACGGAGGGTCACGGTCGTCGGGGGAGGAAATCTCGGGTATCTTCTCTAGTTAGTGAAGAACTTTTTAAGACGCTATTTTTGCATAAAGACGATAGCGCATGCCCGGCGAATGGATTCTATAGTTATCAAGCTTTTATTGAAGCAACGAAGAGGTTCCCTAAATTTGGTGCGACGGGTTGTTTGGCCACACGGAAGAGGGAGATAGCGGCGTTTTTGGCTCAAATTTCGCACGAGACGACGGGTGGGTGGGCGACTGCTCCGGATGGACCTTTTGCTTGGGGGCTGTGCTTCAAAGAGGAAATTAGTCCACAAAGCAACTATTGTGATGATAGTGCTAAGCAGTGGCCTTGCTATCCAGGCAAGTCCTACAAAGGAAGAGGGCCTATTCAACTGTCATGGTAA

mRNA sequence

ATGGCATCCAAAATAATCTTGTTCCTCTCCTTCATCTTCTTCTTTGGAATGGGCCTCCCGTCGATGACGGAGGGTCACGGTCGTCGGGGGAGGAAATCTCGGGTATCTTCTCTAGTTAGTGAAGAACTTTTTAAGACGCTATTTTTGCATAAAGACGATAGCGCATGCCCGGCGAATGGATTCTATAGTTATCAAGCTTTTATTGAAGCAACGAAGAGGTTCCCTAAATTTGGTGCGACGGGTTGTTTGGCCACACGGAAGAGGGAGATAGCGGCGTTTTTGGCTCAAATTTCGCACGAGACGACGGGTGGGTGGGCGACTGCTCCGGATGGACCTTTTGCTTGGGGGCTGTGCTTCAAAGAGGAAATTAGTCCACAAAGCAACTATTGTGATGATAGTGCTAAGCAGTGGCCTTGCTATCCAGGCAAGTCCTACAAAGGAAGAGGGCCTATTCAACTGTCATGGTAA

Coding sequence (CDS)

ATGGCATCCAAAATAATCTTGTTCCTCTCCTTCATCTTCTTCTTTGGAATGGGCCTCCCGTCGATGACGGAGGGTCACGGTCGTCGGGGGAGGAAATCTCGGGTATCTTCTCTAGTTAGTGAAGAACTTTTTAAGACGCTATTTTTGCATAAAGACGATAGCGCATGCCCGGCGAATGGATTCTATAGTTATCAAGCTTTTATTGAAGCAACGAAGAGGTTCCCTAAATTTGGTGCGACGGGTTGTTTGGCCACACGGAAGAGGGAGATAGCGGCGTTTTTGGCTCAAATTTCGCACGAGACGACGGGTGGGTGGGCGACTGCTCCGGATGGACCTTTTGCTTGGGGGCTGTGCTTCAAAGAGGAAATTAGTCCACAAAGCAACTATTGTGATGATAGTGCTAAGCAGTGGCCTTGCTATCCAGGCAAGTCCTACAAAGGAAGAGGGCCTATTCAACTGTCATGGTAA

Protein sequence

MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW

Homology

[Close]

BLAST of Lsi09G007640 vs. ExPASy Swiss-Prot
Match: Q5NB11 (Chitinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht10 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 6.7e-57
Identity = 95/131 (72.52%), Postives = 111/131 (84.73%), Query Frame = 0


Query: 25  GHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLA 84
           G G     S VSS+VSE+L+ +LFLHKDD+ACPA GFY+Y +F+ A  RFP+F ATGC  
Sbjct: 39  GGGYSPSPSPVSSIVSEQLYASLFLHKDDAACPARGFYTYASFVRAATRFPRFAATGCAD 98

Query: 85  TRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPGKS 144
            RKRE+AAFLAQISHETTGGWATAPDGP+AWGLC+KEEI+PQS+YCD + KQWPCYPGKS
Sbjct: 99  ARKREVAAFLAQISHETTGGWATAPDGPYAWGLCYKEEINPQSSYCDATDKQWPCYPGKS 158

Query: 145 YKGRGPIQLSW 156
           Y GRGPIQ+SW
Sbjct: 159 YHGRGPIQISW 169

[Close]

BLAST of Lsi09G007640 vs. ExPASy Swiss-Prot
Match: Q09023 (Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 3.8e-44
Identity = 78/126 (61.90%), Postives = 93/126 (73.81%), Query Frame = 0


Query: 30  GRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLATRKRE 89
           G    +S ++S   F  +  H++D+ACPA GFY+Y AFI A K FP FG TG  ATRK+E
Sbjct: 66  GPTGDLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKE 125

Query: 90  IAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPGKSYKGRG 149
           IAAF  Q SHETTGGWATAPDGP++WG CFK+E +P SNYC  SA +WPC  GKSY GRG
Sbjct: 126 IAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSA-EWPCASGKSYYGRG 185

Query: 150 PIQLSW 156
           P+QLSW
Sbjct: 186 PMQLSW 190

[Close]

BLAST of Lsi09G007640 vs. ExPASy Swiss-Prot
Match: Q9FRV1 (Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 8.5e-44
Identity = 82/120 (68.33%), Postives = 90/120 (75.00%), Query Frame = 0


Query: 35  VSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLATRKREIAAFL 94
           VSS+VS  LF  + LH++D AC A GFY+Y AF+ A   FP FG TG   TRKRE+AAFL
Sbjct: 80  VSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAAGAFPGFGTTGSTDTRKREVAAFL 139

Query: 95  AQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLS 154
           AQ SHETTGGWATAPDG FAWG CFK+E    SNYC  SA QWPC PGKSY GRGPIQLS
Sbjct: 140 AQTSHETTGGWATAPDGAFAWGYCFKQERGATSNYCTPSA-QWPCAPGKSYYGRGPIQLS 198

[Close]

BLAST of Lsi09G007640 vs. ExPASy Swiss-Prot
Match: Q7DNA1 (Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.2e-43
Identity = 76/121 (62.81%), Postives = 92/121 (76.03%), Query Frame = 0


Query: 35  VSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLATRKREIAAFL 94
           V S+V  +LF+ L LH++D ACPA GFY+Y+AF+ A   FP FG TG   TRKRE+AAFL
Sbjct: 89  VGSIVPRDLFERLLLHRNDGACPARGFYTYEAFLAAAAAFPAFGGTGNTETRKREVAAFL 148

Query: 95  AQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLS 154
            Q SHETTGGW TAPDGPF+WG CFK+E +P S+YC  S  +WPC PG+ Y GRGPIQLS
Sbjct: 149 GQTSHETTGGWPTAPDGPFSWGYCFKQEQNPPSDYCQPS-PEWPCAPGRKYYGRGPIQLS 208

Query: 155 W 156
           +
Sbjct: 209 F 208

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BLAST of Lsi09G007640 vs. ExPASy Swiss-Prot
Match: P19171 (Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3)

HSP 1 Score: 175.3 bits (443), Expect = 5.5e-43
Identity = 77/133 (57.89%), Postives = 94/133 (70.68%), Query Frame = 0


Query: 23  TEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGC 82
           T G    G    +S ++S   F  +  H++D+ACPA GFY+Y AFI A K FP FG TG 
Sbjct: 74  TPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGD 133

Query: 83  LATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPG 142
            ATRK+E+AAF  Q SHETTGGWATAPDGP++WG CFK+E +P S+YC+ SA  WPC  G
Sbjct: 134 TATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSA-TWPCASG 193

Query: 143 KSYKGRGPIQLSW 156
           K Y GRGP+QLSW
Sbjct: 194 KRYYGRGPMQLSW 205

[Close]

BLAST of Lsi09G007640 vs. ExPASy TrEMBL
Match: A0A1S3CQS5 (chitinase 10 OS=Cucumis melo OX=3656 GN=LOC103503712 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 6.3e-74
Identity = 135/155 (87.10%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 1   MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANG 60
           M SK +LFLSFI      LPSMTE +GRR +   +SSL+SEELFKTLFLHKDDSACPANG
Sbjct: 1   MTSK-LLFLSFILLLLGSLPSMTESYGRREKICGISSLISEELFKTLFLHKDDSACPANG 60

Query: 61  FYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120
           FYSYQAFIEATKRFPKFG TGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK
Sbjct: 61  FYSYQAFIEATKRFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120

Query: 121 EEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           EEISPQSNYCDDS KQWPCYPGKSYKGRGPIQLSW
Sbjct: 121 EEISPQSNYCDDSVKQWPCYPGKSYKGRGPIQLSW 154

[Close]

BLAST of Lsi09G007640 vs. ExPASy TrEMBL
Match: A0A6J1JXK4 (chitinase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111489215 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.5e-72
Identity = 130/151 (86.09%), Postives = 136/151 (90.07%), Query Frame = 0


Query: 7   LFLSFIFFFGMGLPSMTEGHGRR--GRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSY 66
           LFLSFIF  G GLPS  E HG R    +SRV+SL+SEELFK LFLHKDDSACPANGFYSY
Sbjct: 6   LFLSFIFLLGTGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDDSACPANGFYSY 65

Query: 67  QAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEIS 126
           Q+FIEATK+FPKFG TGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEIS
Sbjct: 66  QSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEIS 125

Query: 127 PQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           PQSNYCDDS +QWPC PGKSYKGRGPIQLSW
Sbjct: 126 PQSNYCDDSVEQWPCSPGKSYKGRGPIQLSW 156

[Close]

BLAST of Lsi09G007640 vs. ExPASy TrEMBL
Match: A0A0A0KPY3 (Glyco_hydro_19_cat domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G128240 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.5e-72
Identity = 132/155 (85.16%), Postives = 137/155 (88.39%), Query Frame = 0


Query: 1   MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANG 60
           M SK  LFLSFI F G  LP MTEGH RR +   +SSL+SEELFKTLFLHKDD ACPANG
Sbjct: 1   MTSK-FLFLSFILFLGTSLPLMTEGHRRREKIYGISSLISEELFKTLFLHKDDGACPANG 60

Query: 61  FYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120
           FYSYQAFIEAT RFPKFG TGCLATRKREIAAFLAQISHETTGGWATAP+GPFAWGLCFK
Sbjct: 61  FYSYQAFIEATWRFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPNGPFAWGLCFK 120

Query: 121 EEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           EEISPQSNYCDDS K+WPC PGKSYKGRGPIQLSW
Sbjct: 121 EEISPQSNYCDDSVKEWPCSPGKSYKGRGPIQLSW 154

[Close]

BLAST of Lsi09G007640 vs. ExPASy TrEMBL
Match: A0A6J1FEL0 (chitinase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111444802 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.9e-71
Identity = 129/152 (84.87%), Postives = 136/152 (89.47%), Query Frame = 0


Query: 6   ILFLSFIFFFGMGLPSMTEGHGRR--GRKSRVSSLVSEELFKTLFLHKDDSACPANGFYS 65
           +LFLSFIF  G GLPS  E HG R    +SRV+SL+SEELFK LFLHKD+SACPANGFYS
Sbjct: 5   LLFLSFIFLLGPGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDNSACPANGFYS 64

Query: 66  YQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEI 125
           YQ+FIEATK+FPKFG TGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEI
Sbjct: 65  YQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEI 124

Query: 126 SPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           SPQSNYCDDS  QWPC PGKSYKGRGPIQLSW
Sbjct: 125 SPQSNYCDDSVTQWPCSPGKSYKGRGPIQLSW 156

[Close]

BLAST of Lsi09G007640 vs. ExPASy TrEMBL
Match: A0A5A7U2X7 (Chitinase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00230 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-69
Identity = 122/134 (91.04%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 22  MTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATG 81
           M E +GRR +   +SSL+SEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFG TG
Sbjct: 1   MIESYGRREKICGISSLISEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGTTG 60

Query: 82  CLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYP 141
           CLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDS KQWPCYP
Sbjct: 61  CLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSVKQWPCYP 120

Query: 142 GKSYKGRGPIQLSW 156
           GKSYKGRGPIQLSW
Sbjct: 121 GKSYKGRGPIQLSW 134

[Close]

BLAST of Lsi09G007640 vs. NCBI nr
Match: XP_038875572.1 (chitinase 10-like [Benincasa hispida])

HSP 1 Score: 298.9 bits (764), Expect = 2.5e-77
Identity = 140/155 (90.32%), Postives = 144/155 (92.90%), Query Frame = 0


Query: 1   MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANG 60
           MASK  LFLSFIF  G GLPSMTEGH RR + SRVSSLVSE+LFKTLFLHKDDSACPANG
Sbjct: 1   MASK-FLFLSFIFLLGTGLPSMTEGHSRREKISRVSSLVSEKLFKTLFLHKDDSACPANG 60

Query: 61  FYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120
           FYSYQAFIEAT RFPKFG TGCLATRKRE+AAFLAQISHETTGGWATAPDGPFAWGLCFK
Sbjct: 61  FYSYQAFIEATTRFPKFGTTGCLATRKREVAAFLAQISHETTGGWATAPDGPFAWGLCFK 120

Query: 121 EEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           EEI+PQSNYCDDS KQWPCYPGKSYKGRGPIQLSW
Sbjct: 121 EEINPQSNYCDDSVKQWPCYPGKSYKGRGPIQLSW 154

[Close]

BLAST of Lsi09G007640 vs. NCBI nr
Match: XP_008466241.1 (PREDICTED: chitinase 10 [Cucumis melo])

HSP 1 Score: 286.6 bits (732), Expect = 1.3e-73
Identity = 135/155 (87.10%), Postives = 140/155 (90.32%), Query Frame = 0


Query: 1   MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANG 60
           M SK +LFLSFI      LPSMTE +GRR +   +SSL+SEELFKTLFLHKDDSACPANG
Sbjct: 1   MTSK-LLFLSFILLLLGSLPSMTESYGRREKICGISSLISEELFKTLFLHKDDSACPANG 60

Query: 61  FYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120
           FYSYQAFIEATKRFPKFG TGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK
Sbjct: 61  FYSYQAFIEATKRFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120

Query: 121 EEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           EEISPQSNYCDDS KQWPCYPGKSYKGRGPIQLSW
Sbjct: 121 EEISPQSNYCDDSVKQWPCYPGKSYKGRGPIQLSW 154

[Close]

BLAST of Lsi09G007640 vs. NCBI nr
Match: XP_022993095.1 (chitinase 10-like [Cucurbita maxima])

HSP 1 Score: 280.4 bits (716), Expect = 9.3e-72
Identity = 130/151 (86.09%), Postives = 136/151 (90.07%), Query Frame = 0


Query: 7   LFLSFIFFFGMGLPSMTEGHGRR--GRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSY 66
           LFLSFIF  G GLPS  E HG R    +SRV+SL+SEELFK LFLHKDDSACPANGFYSY
Sbjct: 6   LFLSFIFLLGTGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDDSACPANGFYSY 65

Query: 67  QAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEIS 126
           Q+FIEATK+FPKFG TGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEIS
Sbjct: 66  QSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEIS 125

Query: 127 PQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           PQSNYCDDS +QWPC PGKSYKGRGPIQLSW
Sbjct: 126 PQSNYCDDSVEQWPCSPGKSYKGRGPIQLSW 156

[Close]

BLAST of Lsi09G007640 vs. NCBI nr
Match: XP_004149478.1 (chitinase 10 [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 9.3e-72
Identity = 132/155 (85.16%), Postives = 137/155 (88.39%), Query Frame = 0


Query: 1   MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANG 60
           M SK  LFLSFI F G  LP MTEGH RR +   +SSL+SEELFKTLFLHKDD ACPANG
Sbjct: 1   MTSK-FLFLSFILFLGTSLPLMTEGHRRREKIYGISSLISEELFKTLFLHKDDGACPANG 60

Query: 61  FYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFK 120
           FYSYQAFIEAT RFPKFG TGCLATRKREIAAFLAQISHETTGGWATAP+GPFAWGLCFK
Sbjct: 61  FYSYQAFIEATWRFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPNGPFAWGLCFK 120

Query: 121 EEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           EEISPQSNYCDDS K+WPC PGKSYKGRGPIQLSW
Sbjct: 121 EEISPQSNYCDDSVKEWPCSPGKSYKGRGPIQLSW 154

[Close]

BLAST of Lsi09G007640 vs. NCBI nr
Match: XP_022938622.1 (chitinase 10-like [Cucurbita moschata])

HSP 1 Score: 277.7 bits (709), Expect = 6.0e-71
Identity = 129/152 (84.87%), Postives = 136/152 (89.47%), Query Frame = 0


Query: 6   ILFLSFIFFFGMGLPSMTEGHGRR--GRKSRVSSLVSEELFKTLFLHKDDSACPANGFYS 65
           +LFLSFIF  G GLPS  E HG R    +SRV+SL+SEELFK LFLHKD+SACPANGFYS
Sbjct: 5   LLFLSFIFLLGPGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDNSACPANGFYS 64

Query: 66  YQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEI 125
           YQ+FIEATK+FPKFG TGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEI
Sbjct: 65  YQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEI 124

Query: 126 SPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           SPQSNYCDDS  QWPC PGKSYKGRGPIQLSW
Sbjct: 125 SPQSNYCDDSVTQWPCSPGKSYKGRGPIQLSW 156

[Close]

BLAST of Lsi09G007640 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 213.4 bits (542), Expect = 1.3e-55
Identity = 98/158 (62.03%), Postives = 117/158 (74.05%), Query Frame = 0


Query: 1   MASKIILFLSFIFFFGMGLPSMTEGHGRRGRKSR---VSSLVSEELFKTLFLHKDDSACP 60
           M  +I L L  + F      S+ E   R+  K +   + SLV   L+  +F+HKD++ACP
Sbjct: 1   MEKQISLLLCLLLFIFSISSSLHETEARKHNKYKPAPIMSLVPRTLYDQIFIHKDNNACP 60

Query: 61  ANGFYSYQAFIEATKRFPKFGATGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGL 120
           A GFY Y+AF+EAT+ FPKFG+ G   TR+RE+AAFLAQISHETTGGWATAPDGP+AWGL
Sbjct: 61  AKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQISHETTGGWATAPDGPYAWGL 120

Query: 121 CFKEEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLSW 156
           CFKEE+SPQSNYCD S K WPC  GKSYKGRGPIQLSW
Sbjct: 121 CFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSW 158

[Close]

BLAST of Lsi09G007640 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 205.3 bits (521), Expect = 3.6e-53
Identity = 82/121 (67.77%), Postives = 105/121 (86.78%), Query Frame = 0


Query: 35  VSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLATRKREIAAFL 94
           +  LV  +L+  +F+HKD++ACPANGFY+Y++F++AT+RFP+FG+ G   T++ E+AAFL
Sbjct: 30  IERLVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAAFL 89

Query: 95  AQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPGKSYKGRGPIQLS 154
           AQISHETTGGWATAPDGP+AWGLCFKEE+SPQS YCD S  QWPC+P K+Y+GRGPIQLS
Sbjct: 90  AQISHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQLS 149

Query: 155 W 156
           W
Sbjct: 150 W 150

[Close]

BLAST of Lsi09G007640 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 175.3 bits (443), Expect = 3.9e-44
Identity = 77/133 (57.89%), Postives = 94/133 (70.68%), Query Frame = 0


Query: 23  TEGHGRRGRKSRVSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGC 82
           T G    G    +S ++S   F  +  H++D+ACPA GFY+Y AFI A K FP FG TG 
Sbjct: 74  TPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGD 133

Query: 83  LATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAKQWPCYPG 142
            ATRK+E+AAF  Q SHETTGGWATAPDGP++WG CFK+E +P S+YC+ SA  WPC  G
Sbjct: 134 TATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSA-TWPCASG 193

Query: 143 KSYKGRGPIQLSW 156
           K Y GRGP+QLSW
Sbjct: 194 KRYYGRGPMQLSW 205

[Close]

BLAST of Lsi09G007640 vs. TAIR 10
Match: AT1G05850.1 (Chitinase family protein )

HSP 1 Score: 102.8 bits (255), Expect = 2.5e-22
Identity = 48/115 (41.74%), Postives = 69/115 (60.00%), Query Frame = 0


Query: 44  FKTLFLHKDDSACPANGFYSYQAFIEATKRFPK--FGATGCLATRKREIAAFLAQISHET 103
           F+ LF  ++     A GF+ YQ+FI A   F    FG TG     ++E+AAFL  ++ +T
Sbjct: 69  FEQLFSKRNTPIAHAVGFWDYQSFITAAALFEPLGFGTTGGKLMGQKEMAAFLGHVASKT 128

Query: 104 TGGWATAPDGPFAWGLCFKEEISPQSNYCDDSAK-QWPCYPGKSYKGRGPIQLSW 156
           + G+  A  GP AWGLC+  E+SP  +YCD+S K ++PC PG  Y GRG + + W
Sbjct: 129 SCGYGVATGGPLAWGLCYNREMSPMQSYCDESWKFKYPCSPGAEYYGRGALPIYW 183

[Close]

BLAST of Lsi09G007640 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 99.4 bits (246), Expect = 2.7e-21
Identity = 53/122 (43.44%), Postives = 72/122 (59.02%), Query Frame = 0


Query: 35  VSSLVSEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGATGCLATRKREIAAFL 94
           V+ +V++E F  + + +  S+C  N FYS  AF+EA   + +FG  G     +REIAAF 
Sbjct: 72  VAEIVTQEFFNGI-ISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFF 131

Query: 95  AQISHETTGGWATAPDGPFAWGLCFKEEISPQS-NYCDDSAKQWPCYPGKSYKGRGPIQL 154
           A ++HET         G F    C+ EEI   S +YCD++A Q+PC P K Y GRGPIQL
Sbjct: 132 AHVTHET---------GHF----CYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQL 179

Query: 155 SW 156
           SW
Sbjct: 192 SW 179

The following BLAST results are available for this feature:

BLAST of Lsi09G007640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5NB11	6.7e-57	72.52	Chitinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht10 PE=2 SV=1	[more]
Q09023	3.8e-44	61.90	Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1	[more]
Q9FRV1	8.5e-44	68.33	Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1	[more]
Q7DNA1	4.2e-43	62.81	Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1	[more]
P19171	5.5e-43	57.89	Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3	[more]

BLAST of Lsi09G007640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CQS5	6.3e-74	87.10	chitinase 10 OS=Cucumis melo OX=3656 GN=LOC103503712 PE=4 SV=1	[more]
A0A6J1JXK4	4.5e-72	86.09	chitinase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111489215 PE=4 SV=1	[more]
A0A0A0KPY3	4.5e-72	85.16	Glyco_hydro_19_cat domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5...	[more]
A0A6J1FEL0	2.9e-71	84.87	chitinase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111444802 PE=4 SV=1	[more]
A0A5A7U2X7	3.6e-69	91.04	Chitinase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00230 P...	[more]

BLAST of Lsi09G007640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875572.1	2.5e-77	90.32	chitinase 10-like [Benincasa hispida]	[more]
XP_008466241.1	1.3e-73	87.10	PREDICTED: chitinase 10 [Cucumis melo]	[more]
XP_022993095.1	9.3e-72	86.09	chitinase 10-like [Cucurbita maxima]	[more]
XP_004149478.1	9.3e-72	85.16	chitinase 10 [Cucumis sativus]	[more]
XP_022938622.1	6.0e-71	84.87	chitinase 10-like [Cucurbita moschata]	[more]

BLAST of Lsi09G007640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01700.1	1.3e-55	62.03	Chitinase family protein	[more]
AT1G02360.1	3.6e-53	67.77	Chitinase family protein	[more]
AT3G12500.1	3.9e-44	57.89	basic chitinase	[more]
AT1G05850.1	2.5e-22	41.74	Chitinase family protein	[more]
AT3G54420.1	2.7e-21	43.44	homolog of carrot EP3-3 chitinase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.530.10		coord: 38..120 e-value: 1.3E-50 score: 174.1
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 121..155 e-value: 1.3E-50 score: 174.1
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 8..155
None	No IPR available	PANTHER	PTHR22595:SF101	OS05G0138200 PROTEIN	coord: 8..155
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 39..155 e-value: 1.59389E-52 score: 164.525
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 38..155 e-value: 1.2E-49 score: 169.2
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 56..78
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 35..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G007640.1	Lsi09G007640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0006952	defense response
biological_process	GO:0000272	polysaccharide catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding