Lsi09G007600 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi09G007600 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 439.9 bits (1130), Expect = 4.3e-122
Identity = 237/524 (45.23%), Postives = 338/524 (64.50%), Query Frame = 0

Query: 31  AAAEDLSPKM--KAISTICSTADYQQECKNTLSNVAHNA-SSNHPKEYVKAAVLATVEEI 90
           +AA+ + P    K I T+CS+  Y Q C+ TL N      + ++P  ++K+A+ A  E++
Sbjct: 95  SAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDL 154

Query: 91  TKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQASYSAVGDSDLHTDADRVADI 150
                  + ++   T N   K ++E CK L++ A +E  AS + +  +++++    V D+
Sbjct: 155 DL---VLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDL 214

Query: 151 KNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTSNALAIVQPSGRKLLGTTEV 210
           ++WLSAV+SYQ++CLDG EE    LK +++ ++  +  LTSN+LA+++     L    +V
Sbjct: 215 ESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPVMKV 274

Query: 211 GS----DGFPTWLSGADRKLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKG 270
                 D  P+W+S  DR++L A       +PNA VAKDGSG + +I  AL A P+  +G
Sbjct: 275 VERHLLDDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTINDALRAMPEKYEG 334

Query: 271 RYVIYVKAGIYDEYITVTKDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDG 330
           RY+IYVK GIYDEY+TV K   N+ M GDG +KTIVTG KS+   I T  TA+F A G+G
Sbjct: 335 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 394

Query: 331 FLCKSMGFSNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGT 390
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ+YR+CVI GT
Sbjct: 395 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 454

Query: 391 VDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAE 450
           +DFIFGD++ I QN  I +R+ +  Q+NTVTAQGR  + + TG V+HNC+I   + L   
Sbjct: 455 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 514

Query: 451 RFKIPTYLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGNFALETCLYLEYGNRGPGAN 510
           + +  +YLGRPWK Y+RT+IMES + + I P G++ W   +FA++T  Y EY N+G   +
Sbjct: 515 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 574

Query: 511 TNRRVRWKGVRVIGRNEALQYTAGPFLLGRAWLPTTGGFYFLGL 547
           T  RV+W G +VI + EAL YT GPFL G  W+  +G    LGL
Sbjct: 575 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of Lsi09G007600 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 8.0e-121
Identity = 243/552 (44.02%), Postives = 336/552 (60.87%), Query Frame = 0

Query: 24  NKSSSSNAAAEDLSPKM--------------KAISTICSTADYQQECKNTLSN-VAHNAS 83
           NKS  S   +E  SPK               K I T+C++  Y+  C+NTL N    +  
Sbjct: 62  NKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTP 121

Query: 84  SNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQAS 143
              P+  +K+A++A  +++ + +    SL    T N   K ++  CK L+  A +EL  S
Sbjct: 122 QTDPRSLLKSAIVAVNDDLDQVFKRVLSL---KTENKDDKDAIAQCKLLVDEAKEELGTS 181

Query: 144 YSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTS 203
              + DS+++  A  V D+ +WLSAV+SYQ++C+DG EE   +LK +++ N   +  LTS
Sbjct: 182 MKRINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTS 241

Query: 204 NALAIVQPSGRKLLGTTEV-------------GSDGFPTWLSGADRKLLAARGGGGQARP 263
           N+LA+++     L    +V              +D   +WLS  +R++L A       +P
Sbjct: 242 NSLAMIKSLDGYLSSVPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKA-LKP 301

Query: 264 NAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDGPR 323
           NA VAKDGSG + +I AAL A P   +GRY IY+K GIYDE + + K   NV M GDG +
Sbjct: 302 NATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQ 361

Query: 324 KTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSAFF 383
           KTIVTG KS+   I T  TA+F A G+GF+ +SMGF NTAGPEGHQAVA+RVQSDRS F 
Sbjct: 362 KTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFL 421

Query: 384 NCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTA 443
           NCR +G+QDTLY  THRQ+YR+CVI GTVDFIFGD++ I QN  I +R+ +  Q+NTVTA
Sbjct: 422 NCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTA 481

Query: 444 QGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQPA 503
           QGR  + + TG VIHNC + P + L   + +  +YLGRPWK ++RTV+MEST+ D I P 
Sbjct: 482 QGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPV 541

Query: 504 GYMPW-SGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLGRAW 547
           G++ W   +FA++T  Y EY N GP   T  RV+W G RV+ + EA+++T GPFL G  W
Sbjct: 542 GWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-W 601

BLAST of Lsi09G007600 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 2.3e-120
Identity = 224/553 (40.51%), Postives = 339/553 (61.30%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKNTL 63
           + V+  +S +L++ + +AV   V+ + S NA  E+++  +KAI  +C+  DY++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQF 123
              A + S   P E VK A  AT+++I+     S + M+E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTSD--PLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNL 183
           AI EL  S+  +G  + H   + +  ++ WLSA IS++Q+CLDG +       E ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 GVAAKLTSNALAIVQPSGRKL-------LGTTEVGSDGFPTWLSGADRKLLAARGGGGQA 243
             A +LT N LA+V      L       + +  + S  FP+W+    R+LL A     + 
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNA--PMSEV 254

Query: 244 RPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDG 303
           +P+ VVA+DGSGQYK+I  AL   PK     +V+++K GIY EY+ V + M ++   GDG
Sbjct: 255 KPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDG 314

Query: 304 PRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSA 363
           P KT+++G KS +DGITT  TA+ + +GD F+ K++ F NTAG   HQAVA+RV +D S 
Sbjct: 315 PDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESI 374

Query: 364 FFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTV 423
           F+NC+ DG+QDTLY  +HRQFYR+C ISGT+DF+FGD++ + QN  ++VR+P+ NQ   +
Sbjct: 375 FYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPI 434

Query: 424 TAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQ 483
           TA GR   +E TG V+  C IV E    A + +  TYLGRPWK+Y+RT+IM + + DF+ 
Sbjct: 435 TAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVP 494

Query: 484 PAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLGRA 543
           P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E L++T   ++ G A
Sbjct: 495 PEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDA 554

Query: 544 WLPTTGGFYFLGL 547
           W+P  G  Y LGL
Sbjct: 555 WIPGKGVPYILGL 562

BLAST of Lsi09G007600 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 433.3 bits (1113), Expect = 4.0e-120
Identity = 216/555 (38.92%), Postives = 339/555 (61.08%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV-----VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKN 63
           + +V  +S +L++ + +AV     +NK    +    +++  +KA+  +C+  DY++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLL 123
           TL  + +  ++  P E VK A   T+++IT     S ++M E   ++  +M+++ CK+L+
Sbjct: 74  TL--IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQD 183
            +A+DEL  S+  +G  + H   + + +++ WLSA IS++++CL+G +       E M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 NLGVAAKLTSNALAIVQPSGRKL-------LGTTEVGSDGFPTWLSGADRKLLAARGGGG 243
            L  A +LT N LAI+      +       L +  + ++GFP+W+    RKLL A     
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQRGRKLLQAAAAYS 253

Query: 244 QARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYG 303
             +P+ VVA+DGSGQYK+I  AL   PK     +V+++KAG+Y EY+ V K M ++   G
Sbjct: 254 DVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIG 313

Query: 304 DGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDR 363
           DGP KTI++G K+ +DGITT  TA+ + +G+ F+ K++GF NTAG   HQAVA+RVQSD 
Sbjct: 314 DGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDE 373

Query: 364 SAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQN 423
           S FFNCR DG+QDTLY  +HRQF+R+C ISGT+DF+FGD++ + QN  ++VR+P+ NQ  
Sbjct: 374 SIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQAC 433

Query: 424 TVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDF 483
            +TA GR   +E TG V   C I  E    A +     YLGRPWK+Y+RT+IM + + DF
Sbjct: 434 PITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDF 493

Query: 484 IQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLG 543
           +QP G+ PW G+F L+T  Y E  N GPG+    RV W G++ +   + L++T   ++ G
Sbjct: 494 VQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQG 553

Query: 544 RAWLPTTGGFYFLGL 547
             W+P  G  Y  GL
Sbjct: 554 DDWIPGKGVPYTTGL 565

BLAST of Lsi09G007600 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 3.0e-115
Identity = 240/569 (42.18%), Postives = 335/569 (58.88%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           M+GKVVVS  S++L+VGVA+ VV   NK+  +N     LSP+MKA+  IC +   +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDAN-----LSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVE--ATNNATIKMSVEDCK 120
            TL  V     S  P + +KA +LAT +E+TK  NF+    V   ++ +   K  ++ CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEK 180
            +  +A+++L      +G+ DL     ++  +K WL  V +YQ  CLD +EE D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDNLGVAAKLTSNAL---------------------------------------AIVQP 240
           + + +  +  LT+NA+                                        +  P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RKLLGTTEVGSDGFPTWLSGADRKLLA--------ARGGGGQARPNAVVAKDGSGQ 300
            G  R+LL   ++   G PTW+SGADRKL+A         RGGG + R N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 YKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDGPRKTIVTGRKS-- 360
           +K++  A+ A P+  +GR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQD 420
              G TT  +A+     +GF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAQQKEI 480
           TLYV   RQFYRNCV+SGTVDFIFG S+T+IQN+LI+VR+    Q NTVTA G      +
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 -TGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGN 513
             G+V+ NCRIVP++KL  ER  + TYLGRPWK+++ TVIM + +GD I+P G+  W G 
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

BLAST of Lsi09G007600 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.3e-278
Identity = 483/567 (85.19%), Postives = 519/567 (91.53%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VAHN SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI                VQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007600 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.0e-276
Identity = 481/567 (84.83%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VA N SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI                VQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007600 vs. ExPASy TrEMBL
Match: A0A0A0KJ73 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G125700 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.6e-273
Identity = 473/567 (83.42%), Postives = 516/567 (91.01%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           M+GKV+VSGLSLILVVG+ALA+   +NKS++SN   ED+SPKMKA+S ICST DYQ ECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
            TL +VA N SSN PK+Y +AA+LAT+ EITKGYN SDSL+VEA+ NA+IKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYSAVG+SDLHTD+DRVADIKNWLSAVISYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI                VQPSGR+LLGTT V  DGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++  G +  PNAVVAKDGSG+YK+IAAALAAYPK L+GRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG +VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           QYTAG FLLGR+WLPTTGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of Lsi09G007600 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.8e-264
Identity = 453/567 (79.89%), Postives = 508/567 (89.59%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MV KV++ G+SLILVVGVALAV   V+ + SS+AA EDLSPKMKA+STIC+ A+YQQEC+
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTLSN AHNASS+ PKEYV+AA+L+T+EE+ KGYN +D  M+EA NN +IKM VEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAID+LQASYS VG+ DLHT+ADRVADIKNWL++VISYQQSCLDGLEEFDPQLK+KMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DNLGVAAKLTSNALAIV----------------QPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L  A KLTSNALAIV                QPSGR+LLGTTEVG DG+PTWL+GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA+RGGG + +PNAVVAKDGSGQ+K+I+AALAAYPK L+GRYVIYVKAGIY+EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+N+FMYGDGP+KTIVTG KSNR G TTQ+TA+F A+G+GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA  KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQP GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           Q+TAGPFL GRAWLPTTGG Y LGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lsi09G007600 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.5e-263
Identity = 452/567 (79.72%), Postives = 508/567 (89.59%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MV KV++ G++LILVVGVALAV   V+ + SS+AA EDLSPKMKA+STIC+ A+YQQEC+
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTLSN AHNASS+ PKEYV+AA+L+T+EE+ KGYN +D  M+EA NN +IKM VEDC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAID+LQASYS VG+ DLHT+ADRVADIKNWL++VISYQQSCLDGLEEFDPQLK+KMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DNLGVAAKLTSNALAIV----------------QPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L  A KLTSNALAIV                QPSGR+LLGTTEVG DG+PTWL+GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA+RGGG + +PNAVVAKDGSGQ+K+I+AALAAYPK L+GRYVIYVKAGIY+EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+N+FMYGDGP+KTIVTG KSNR G TTQ+TA+F A+G+GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA  KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQP GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           Q+TAGPFL GRAWLPTTGG Y LGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lsi09G007600 vs. NCBI nr
Match: XP_038875889.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 986.5 bits (2549), Expect = 9.3e-284
Identity = 500/568 (88.03%), Postives = 528/568 (92.96%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKVVVSGLSLILVVGVALAV   VNKS+SSNAA E  SPKMKAISTICST+DYQ+ECK
Sbjct: 1   MVGKVVVSGLSLILVVGVALAVVATVNKSNSSNAALE--SPKMKAISTICSTSDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTLSNVAHNA+SN PK+Y KAAVLAT+EEITKGYNFSD+LMVEA NNA+ KMSVEDCKDL
Sbjct: 61  NTLSNVAHNATSNDPKDYAKAAVLATIEEITKGYNFSDTLMVEAANNASTKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQ SYSA+GDSDLHTD DRVADI NWLSAVISYQQSCLDGLEEF+PQLK+KMQ
Sbjct: 121 LQFAIDELQESYSAIGDSDLHTDTDRVADINNWLSAVISYQQSCLDGLEEFNPQLKQKMQ 180

Query: 181 DNLGVAAKLTSNALAI-----------------VQPSGRKLLGTTEVGSDGFPTWLSGAD 240
           DNL VA+KLTSNALA+                 VQP+GR+LLGTTEVGSDGFP WLSGAD
Sbjct: 181 DNLSVASKLTSNALAVVSAVSNILDNYRLQLKQVQPNGRRLLGTTEVGSDGFPIWLSGAD 240

Query: 241 RKLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITV 300
           RKLLAARG GG+ARPNAVVAKDGSGQYKSIAAALAAYPK LKGRYVIYVKAGIYDE+I V
Sbjct: 241 RKLLAARGRGGRARPNAVVAKDGSGQYKSIAAALAAYPKALKGRYVIYVKAGIYDEHIIV 300

Query: 301 TKDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGH 360
           TKDM+NVFMYGDGPRKTIVTGRK+NRDG TTQNTASF+ALG+GFLCKSMGF+NTAGPEGH
Sbjct: 301 TKDMKNVFMYGDGPRKTIVTGRKNNRDGFTTQNTASFAALGNGFLCKSMGFTNTAGPEGH 360

Query: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLI 420
           QAVALRVQSDRSAFFNCRMDGHQDTLY QTHRQFYRNCVISGTVDFIFGDS+T+IQNSLI
Sbjct: 361 QAVALRVQSDRSAFFNCRMDGHQDTLYAQTHRQFYRNCVISGTVDFIFGDSTTLIQNSLI 420

Query: 421 IVRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYAR 480
           IVRRPMDNQQNTVTAQGR  QK+ITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYAR
Sbjct: 421 IVRRPMDNQQNTVTAQGRDLQKQITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYAR 480

Query: 481 TVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA 540
           TVIME+TLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA
Sbjct: 481 TVIMETTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEA 540

Query: 541 LQYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           LQYTAGPFLLGRAWLP+TGG YFLGLKN
Sbjct: 541 LQYTAGPFLLGRAWLPSTGGPYFLGLKN 566

BLAST of Lsi09G007600 vs. NCBI nr
Match: KAA0050274.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 966.1 bits (2496), Expect = 1.3e-277
Identity = 483/567 (85.19%), Postives = 519/567 (91.53%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VAHN SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI                VQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007600 vs. NCBI nr
Match: XP_008466403.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 961.1 bits (2483), Expect = 4.2e-276
Identity = 481/567 (84.83%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           MVGKV+VS LSLILVVG+ALAVV   NK + SN   E++SPKMKAIS ICST DYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
           NTL++VA N SSN PK+Y KAA+LAT++EITKGYN SDSL+VEA NNA+IKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYS VG+SDLHTDADR+ADIKNWLSAV+SYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI                VQPSGR+LLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++ GG +  PNAVVAKDGSGQYK+IAAALAAYPK L+GRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           QYTAG FLLGR WLP TGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007600 vs. NCBI nr
Match: XP_004149499.1 (pectinesterase [Cucumis sativus] >KGN49780.1 hypothetical protein Csa_004678 [Cucumis sativus])

HSP 1 Score: 950.3 bits (2455), Expect = 7.4e-273
Identity = 473/567 (83.42%), Postives = 516/567 (91.01%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           M+GKV+VSGLSLILVVG+ALA+   +NKS++SN   ED+SPKMKA+S ICST DYQ ECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDL 120
            TL +VA N SSN PK+Y +AA+LAT+ EITKGYN SDSL+VEA+ NA+IKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQ 180
           LQFAIDELQASYSAVG+SDLHTD+DRVADIKNWLSAVISYQQSCLDGL EFDPQLK++MQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DNLGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADR 240
           D L VA KLTSNALAI                VQPSGR+LLGTT V  DGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVT 300
           KLLA++  G +  PNAVVAKDGSG+YK+IAAALAAYPK L+GRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQ 360
           KDM+NVFMYGDGPRKTIVTGRKSNRDG TTQNTASF+A+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRA+QKEITGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG +VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGPFLLGRAWLPTTGGFYFLGLKN 549
           QYTAG FLLGR+WLPTTGG Y+LGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of Lsi09G007600 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 941.0 bits (2431), Expect = 4.5e-270
Identity = 474/565 (83.89%), Postives = 510/565 (90.27%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVVNKSSSSNAAAEDL-SPKMKAISTICSTADYQQECKNT 60
           MVGKVVVSG+SLILVVGVALAVV   + +N +  DL SPKMKA+STICST DYQQEC+NT
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDVDLSSPKMKAVSTICSTTDYQQECQNT 60

Query: 61  LSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQ 120
           L+NVA NAS + PKEY KAAVLAT++EITKGYN +  LMVEA NNA+IKMSV+DCKDLLQ
Sbjct: 61  LTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLLQ 120

Query: 121 FAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDN 180
            AIDEL ASYS VGD DLHT+ DRVADIKNWL+AVISYQQSCLDGLEEFDPQLK+KMQD 
Sbjct: 121 SAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 180

Query: 181 LGVAAKLTSNALAI----------------VQPSGRKLLGTTEVGSDGFPTWLSGADRKL 240
           L VA KLTSNALAI                VQPSGR+LL TTE+GSDGFPTW+SGADRKL
Sbjct: 181 LDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRKL 240

Query: 241 LAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKD 300
           LA+RGGG + +PNAVVAKDGSGQYKSI AALAAYPK L+GRYVIYVKAGIYDEYITVTKD
Sbjct: 241 LASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTKD 300

Query: 301 MRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAV 360
           M+NVFMYGDGPRKTIVTGRK+NRDGITTQNTASFSA+G+GFLCKSMGF NTAGPEGHQAV
Sbjct: 301 MKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQAV 360

Query: 361 ALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 420
           ALRVQSDRSAF+NCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR
Sbjct: 361 ALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVR 420

Query: 421 RPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVI 480
           RPMDNQ+NTVTA GRA +KEI+GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYARTVI
Sbjct: 421 RPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVI 480

Query: 481 MESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 540
           ME+TLGDFIQP GYMPWSGNFALETC Y EYGNRGPGANTNRRVRWKGVRVIGRNEALQY
Sbjct: 481 METTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQY 540

Query: 541 TAGPFLLGRAWLPTTGGFYFLGLKN 549
           TAGPFL+GRAWLP TGG Y LGLK+
Sbjct: 541 TAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of Lsi09G007600 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.9 bits (1130), Expect = 3.0e-123
Identity = 237/524 (45.23%), Postives = 338/524 (64.50%), Query Frame = 0

Query: 31  AAAEDLSPKM--KAISTICSTADYQQECKNTLSNVAHNA-SSNHPKEYVKAAVLATVEEI 90
           +AA+ + P    K I T+CS+  Y Q C+ TL N      + ++P  ++K+A+ A  E++
Sbjct: 95  SAAQSVKPGQGDKIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDL 154

Query: 91  TKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQASYSAVGDSDLHTDADRVADI 150
                  + ++   T N   K ++E CK L++ A +E  AS + +  +++++    V D+
Sbjct: 155 DL---VLEKVLSLKTENQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDL 214

Query: 151 KNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTSNALAIVQPSGRKLLGTTEV 210
           ++WLSAV+SYQ++CLDG EE    LK +++ ++  +  LTSN+LA+++     L    +V
Sbjct: 215 ESWLSAVMSYQETCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTENLSPVMKV 274

Query: 211 GS----DGFPTWLSGADRKLLAARGGGGQARPNAVVAKDGSGQYKSIAAALAAYPKTLKG 270
                 D  P+W+S  DR++L A       +PNA VAKDGSG + +I  AL A P+  +G
Sbjct: 275 VERHLLDDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTINDALRAMPEKYEG 334

Query: 271 RYVIYVKAGIYDEYITVTKDMRNVFMYGDGPRKTIVTGRKSNRDGITTQNTASFSALGDG 330
           RY+IYVK GIYDEY+TV K   N+ M GDG +KTIVTG KS+   I T  TA+F A G+G
Sbjct: 335 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 394

Query: 331 FLCKSMGFSNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGT 390
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ+YR+CVI GT
Sbjct: 395 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 454

Query: 391 VDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAQQKEITGLVIHNCRIVPEQKLFAE 450
           +DFIFGD++ I QN  I +R+ +  Q+NTVTAQGR  + + TG V+HNC+I   + L   
Sbjct: 455 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 514

Query: 451 RFKIPTYLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGNFALETCLYLEYGNRGPGAN 510
           + +  +YLGRPWK Y+RT+IMES + + I P G++ W   +FA++T  Y EY N+G   +
Sbjct: 515 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 574

Query: 511 TNRRVRWKGVRVIGRNEALQYTAGPFLLGRAWLPTTGGFYFLGL 547
           T  RV+W G +VI + EAL YT GPFL G  W+  +G    LGL
Sbjct: 575 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of Lsi09G007600 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 435.6 bits (1119), Expect = 5.7e-122
Identity = 243/552 (44.02%), Postives = 336/552 (60.87%), Query Frame = 0

Query: 24  NKSSSSNAAAEDLSPKM--------------KAISTICSTADYQQECKNTLSN-VAHNAS 83
           NKS  S   +E  SPK               K I T+C++  Y+  C+NTL N    +  
Sbjct: 62  NKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTP 121

Query: 84  SNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQFAIDELQAS 143
              P+  +K+A++A  +++ + +    SL    T N   K ++  CK L+  A +EL  S
Sbjct: 122 QTDPRSLLKSAIVAVNDDLDQVFKRVLSL---KTENKDDKDAIAQCKLLVDEAKEELGTS 181

Query: 144 YSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNLGVAAKLTS 203
              + DS+++  A  V D+ +WLSAV+SYQ++C+DG EE   +LK +++ N   +  LTS
Sbjct: 182 MKRINDSEVNNFAKIVPDLDSWLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTS 241

Query: 204 NALAIVQPSGRKLLGTTEV-------------GSDGFPTWLSGADRKLLAARGGGGQARP 263
           N+LA+++     L    +V              +D   +WLS  +R++L A       +P
Sbjct: 242 NSLAMIKSLDGYLSSVPKVKTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKA-LKP 301

Query: 264 NAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDGPR 323
           NA VAKDGSG + +I AAL A P   +GRY IY+K GIYDE + + K   NV M GDG +
Sbjct: 302 NATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQ 361

Query: 324 KTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSAFF 383
           KTIVTG KS+   I T  TA+F A G+GF+ +SMGF NTAGPEGHQAVA+RVQSDRS F 
Sbjct: 362 KTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFL 421

Query: 384 NCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTA 443
           NCR +G+QDTLY  THRQ+YR+CVI GTVDFIFGD++ I QN  I +R+ +  Q+NTVTA
Sbjct: 422 NCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTA 481

Query: 444 QGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQPA 503
           QGR  + + TG VIHNC + P + L   + +  +YLGRPWK ++RTV+MEST+ D I P 
Sbjct: 482 QGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPV 541

Query: 504 GYMPW-SGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLGRAW 547
           G++ W   +FA++T  Y EY N GP   T  RV+W G RV+ + EA+++T GPFL G  W
Sbjct: 542 GWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-W 601

BLAST of Lsi09G007600 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 434.1 bits (1115), Expect = 1.7e-121
Identity = 224/553 (40.51%), Postives = 339/553 (61.30%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV---VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKNTL 63
           + V+  +S +L++ + +AV   V+ + S NA  E+++  +KAI  +C+  DY++ C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLLQF 123
              A + S   P E VK A  AT+++I+     S + M+E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTSD--PLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQDNL 183
           AI EL  S+  +G  + H   + +  ++ WLSA IS++Q+CLDG +       E ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 GVAAKLTSNALAIVQPSGRKL-------LGTTEVGSDGFPTWLSGADRKLLAARGGGGQA 243
             A +LT N LA+V      L       + +  + S  FP+W+    R+LL A     + 
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNA--PMSEV 254

Query: 244 RPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDG 303
           +P+ VVA+DGSGQYK+I  AL   PK     +V+++K GIY EY+ V + M ++   GDG
Sbjct: 255 KPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDG 314

Query: 304 PRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSA 363
           P KT+++G KS +DGITT  TA+ + +GD F+ K++ F NTAG   HQAVA+RV +D S 
Sbjct: 315 PDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESI 374

Query: 364 FFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTV 423
           F+NC+ DG+QDTLY  +HRQFYR+C ISGT+DF+FGD++ + QN  ++VR+P+ NQ   +
Sbjct: 375 FYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPI 434

Query: 424 TAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQ 483
           TA GR   +E TG V+  C IV E    A + +  TYLGRPWK+Y+RT+IM + + DF+ 
Sbjct: 435 TAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVP 494

Query: 484 PAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLGRA 543
           P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E L++T   ++ G A
Sbjct: 495 PEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDA 554

Query: 544 WLPTTGGFYFLGL 547
           W+P  G  Y LGL
Sbjct: 555 WIPGKGVPYILGL 562

BLAST of Lsi09G007600 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 433.3 bits (1113), Expect = 2.8e-121
Identity = 216/555 (38.92%), Postives = 339/555 (61.08%), Query Frame = 0

Query: 4   KVVVSGLSLILVVGVALAV-----VNKSSSSNAAAEDLSPKMKAISTICSTADYQQECKN 63
           + +V  +S +L++ + +AV     +NK    +    +++  +KA+  +C+  DY++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVEATNNATIKMSVEDCKDLL 123
           TL  + +  ++  P E VK A   T+++IT     S ++M E   ++  +M+++ CK+L+
Sbjct: 74  TL--IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEKMQD 183
            +A+DEL  S+  +G  + H   + + +++ WLSA IS++++CL+G +       E M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 NLGVAAKLTSNALAIVQPSGRKL-------LGTTEVGSDGFPTWLSGADRKLLAARGGGG 243
            L  A +LT N LAI+      +       L +  + ++GFP+W+    RKLL A     
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQRGRKLLQAAAAYS 253

Query: 244 QARPNAVVAKDGSGQYKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYG 303
             +P+ VVA+DGSGQYK+I  AL   PK     +V+++KAG+Y EY+ V K M ++   G
Sbjct: 254 DVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIG 313

Query: 304 DGPRKTIVTGRKSNRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDR 363
           DGP KTI++G K+ +DGITT  TA+ + +G+ F+ K++GF NTAG   HQAVA+RVQSD 
Sbjct: 314 DGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDE 373

Query: 364 SAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQN 423
           S FFNCR DG+QDTLY  +HRQF+R+C ISGT+DF+FGD++ + QN  ++VR+P+ NQ  
Sbjct: 374 SIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQAC 433

Query: 424 TVTAQGRAQQKEITGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDF 483
            +TA GR   +E TG V   C I  E    A +     YLGRPWK+Y+RT+IM + + DF
Sbjct: 434 PITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDF 493

Query: 484 IQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVRVIGRNEALQYTAGPFLLG 543
           +QP G+ PW G+F L+T  Y E  N GPG+    RV W G++ +   + L++T   ++ G
Sbjct: 494 VQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQYIQG 553

Query: 544 RAWLPTTGGFYFLGL 547
             W+P  G  Y  GL
Sbjct: 554 DDWIPGKGVPYTTGL 565

BLAST of Lsi09G007600 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 417.2 bits (1071), Expect = 2.1e-116
Identity = 240/569 (42.18%), Postives = 335/569 (58.88%), Query Frame = 0

Query: 1   MVGKVVVSGLSLILVVGVALAVV---NKSSSSNAAAEDLSPKMKAISTICSTADYQQECK 60
           M+GKVVVS  S++L+VGVA+ VV   NK+  +N     LSP+MKA+  IC +   +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDAN-----LSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLSNVAHNASSNHPKEYVKAAVLATVEEITKGYNFSDSLMVE--ATNNATIKMSVEDCK 120
            TL  V     S  P + +KA +LAT +E+TK  NF+    V   ++ +   K  ++ CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSAVGDSDLHTDADRVADIKNWLSAVISYQQSCLDGLEEFDPQLKEK 180
            +  +A+++L      +G+ DL     ++  +K WL  V +YQ  CLD +EE D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDNLGVAAKLTSNAL---------------------------------------AIVQP 240
           + + +  +  LT+NA+                                        +  P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RKLLGTTEVGSDGFPTWLSGADRKLLA--------ARGGGGQARPNAVVAKDGSGQ 300
            G  R+LL   ++   G PTW+SGADRKL+A         RGGG + R N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 YKSIAAALAAYPKTLKGRYVIYVKAGIYDEYITVTKDMRNVFMYGDGPRKTIVTGRKS-- 360
           +K++  A+ A P+  +GR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGITTQNTASFSALGDGFLCKSMGFSNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQD 420
              G TT  +A+     +GF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAQQKEI 480
           TLYV   RQFYRNCV+SGTVDFIFG S+T+IQN+LI+VR+    Q NTVTA G      +
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 -TGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGN 513
             G+V+ NCRIVP++KL  ER  + TYLGRPWK+++ TVIM + +GD I+P G+  W G 
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540