Lsi09G007590 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G007590
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPectinesterase
Locationchr09: 8689791 .. 8692239 (+)
RNA-Seq ExpressionLsi09G007590
SyntenyLsi09G007590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTACAAACCCCTCCCAAAAAAAAAAAAAGTACTTCCTCCACAAAACCCACCCTCTTTTTTTCCCATGTTTATGGTGGGAAAGTATTTTTCATCTGGGTTTTGATCAATTTTTCATAATTTGCAGGAGGAGGAACATAGGAGGGAACAAAAAAATAAAATAAAATAAAATGGTGAATAAGGTGGTCTTGCCGGGAATATCCCTTATTCTTGTTGTCGGTGTAGCACTTGCTGTTGTTGCAGTGGTTAACAAGAACAATTCTGACACTTCGACAGAGAATCTTTCCCCAAAAATGAAGGCAGTTTCAACCATTTGTTCTACTACTAATTACCAAGAAGAATGTCAAAATACACTTAGCAATGCTGCTCATAATGCCTCTTCAGATGACCCTAAAGAGTATGTTAAAGCGGCAATTCTTGCAACTATTGAAGAGGTTAAAAAGGGTTATAACTTGACTGATGGCTTTAAGGTTGAGGCTGCTAACAATGCCAGCATCAAAATGGGTGTTGAGGATTGCAGGGACTTATTGCAATTCGCCATTGACCAGTTGCAGGCATCTTACACCACAGTTGGCGAACCCGATTTGCATACCAATGCCGATCGTGTCGCTGATATCAAGAATTGGCTCACTGCTGTCATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCCCAGCTTAAACAGAAGATGCAAGATGGTCTTGATGTTGCTAGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGACATTCTTGCAAACTTCGGATTGCAATTGAAGGTAATTATGTTCAGAGCAATGATTAAGTGAGCAATGAAAGGCTGCATCTAATCTCATTCTCCTGCTTCCTCCCTTCCCTTTCTTTTATTCCTCCCTTCCCTTTCTTTTATAAAAGACACTGACTGCACCCATCTCTTGTGGAGTGGAGACATCACCCAATGAAAGGCTGTCATTTCCTAATATGATCTATTTTTGTTAGCAGGTTCAACCAAGTGGTCGGAGGCTTCTTGGAACAATAGAGGTAGGGAGCGACGGGTTCCCGACATGGATGTCAGGGGCAGACAGGAAGTTGCTAGCATCGAGAGGAGGAGGCGCTCGAGTGAAGCCGAATGCAGTAGTGGCCAAGGACGGTAGCGGACAGTTCAAGACCATTGCTGCTGCTTTGGCTGCATACCCAAAGGTCTTAAGAGGAAGATATGTAATCTACGTAAAAGCAGGGATCTATGATGAATACGTCACTGTAACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAAAAAACCATTGTCACTGGTCGCAAGAGCAACCGTGATGGCTTCACCACCCAAGACACTGCCTCTTTTATAGCCTTAGGAGACGGATTTCTCTGCAAATCAATGGGATTCCGTAACACAGCTGGCCCTGAAGGTCACCAAGCCGTAGCTCTTAGAGTCCAATCTGATAGATCCGCCTTCTTTAATTGCAGAATGGATGGCTACCAAGACACTCTTTACGTTCAAACTCAACGTCAATTCTACCGCAATTGCGTCATCTCCGGCACTGTAGATTTCATCTTCGGCGACTCAACCACCATCATCCAAAACTCCTTGATCATCGTCCGTCGCCCCATGGACAACCAACAGAACACCGTCACGGCCAATGGTCGCGCGGATCCAAAAGAAGTCTCAGGCTTGGTGATTCACAACTGCAGAATCGTGCCAGAACAGAAGCTGTTTGCTGAGAGGTTCAAGATTCCTACATACTTGGGACGGCCATGGAAGCAATATGCTAGAACTGTGATCATGGAGTCGACATTGGGTGACTTCATTAAGCCAGACGGTTACATGCCATGGTCTGGAAATTTTGCATTGGAAACTTGTTTGTATTTGGAGTATGGAAACAGAGGACCTGGGGCTAACACTAACAGGAGAGTAAGATGGAAGGGTGTTAAAGTTATTCCAAAGAATGAAGCTATGAAATTCACTGCTGGACCGTTTCTTCAAGGAGGATCTTGGCTTCCAACCACTGGTGGACCTTACCTTTTGGGCTTGAAGAACTGAAAAAGAAAAATTGTGTTTGTTGATGAAGAATGTAATATAATTTATTTGATTCCCCCATTCTACAATTATTTATGCCTTAGATCTTAGTTGGTATTTTGTAAGCAATTGCAAATGGCCTTATATGGTCACAACTTTGAGAAGGCGTGCAGGTTAGTAACAAATAAACAACATTTCATTATTACTATTTTTAAACTCTTATAAAAGTAAGATTTATTCTTAATAATAGAGATGTCCATGAGACCAGAAATAGACATTTCTCATTGTCACTTAAATAAAATAAATGGTCACAACTTCTTTAGTTTGGTGGGTATATTTCTCATTGTCACTTGAATAAAATAAATAAATAAATAATCAGATCTCCATTTTTTATATCTCAC

mRNA sequence

CATTACAAACCCCTCCCAAAAAAAAAAAAAGTACTTCCTCCACAAAACCCACCCTCTTTTTTTCCCATGTTTATGGTGGGAAAGTATTTTTCATCTGGGTTTTGATCAATTTTTCATAATTTGCAGGAGGAGGAACATAGGAGGGAACAAAAAAATAAAATAAAATAAAATGGTGAATAAGGTGGTCTTGCCGGGAATATCCCTTATTCTTGTTGTCGGTGTAGCACTTGCTGTTGTTGCAGTGGTTAACAAGAACAATTCTGACACTTCGACAGAGAATCTTTCCCCAAAAATGAAGGCAGTTTCAACCATTTGTTCTACTACTAATTACCAAGAAGAATGTCAAAATACACTTAGCAATGCTGCTCATAATGCCTCTTCAGATGACCCTAAAGAGTATGTTAAAGCGGCAATTCTTGCAACTATTGAAGAGGTTAAAAAGGGTTATAACTTGACTGATGGCTTTAAGGTTGAGGCTGCTAACAATGCCAGCATCAAAATGGGTGTTGAGGATTGCAGGGACTTATTGCAATTCGCCATTGACCAGTTGCAGGCATCTTACACCACAGTTGGCGAACCCGATTTGCATACCAATGCCGATCGTGTCGCTGATATCAAGAATTGGCTCACTGCTGTCATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCCCAGCTTAAACAGAAGATGCAAGATGGTCTTGATGTTGCTAGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGACATTCTTGCAAACTTCGGATTGCAATTGAAGGTTCAACCAAGTGGTCGGAGGCTTCTTGGAACAATAGAGGTAGGGAGCGACGGGTTCCCGACATGGATGTCAGGGGCAGACAGGAAGTTGCTAGCATCGAGAGGAGGAGGCGCTCGAGTGAAGCCGAATGCAGTAGTGGCCAAGGACGGTAGCGGACAGTTCAAGACCATTGCTGCTGCTTTGGCTGCATACCCAAAGGTCTTAAGAGGAAGATATGTAATCTACGTAAAAGCAGGGATCTATGATGAATACGTCACTGTAACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAAAAAACCATTGTCACTGGTCGCAAGAGCAACCGTGATGGCTTCACCACCCAAGACACTGCCTCTTTTATAGCCTTAGGAGACGGATTTCTCTGCAAATCAATGGGATTCCGTAACACAGCTGGCCCTGAAGGTCACCAAGCCGTAGCTCTTAGAGTCCAATCTGATAGATCCGCCTTCTTTAATTGCAGAATGGATGGCTACCAAGACACTCTTTACGTTCAAACTCAACGTCAATTCTACCGCAATTGCGTCATCTCCGGCACTGTAGATTTCATCTTCGGCGACTCAACCACCATCATCCAAAACTCCTTGATCATCGTCCGTCGCCCCATGGACAACCAACAGAACACCGTCACGGCCAATGGTCGCGCGGATCCAAAAGAAGTCTCAGGCTTGGTGATTCACAACTGCAGAATCGTGCCAGAACAGAAGCTGTTTGCTGAGAGGTTCAAGATTCCTACATACTTGGGACGGCCATGGAAGCAATATGCTAGAACTGTGATCATGGAGTCGACATTGGGTGACTTCATTAAGCCAGACGGTTACATGCCATGGTCTGGAAATTTTGCATTGGAAACTTGTTTGTATTTGGAGTATGGAAACAGAGGACCTGGGGCTAACACTAACAGGAGAGTAAGATGGAAGGGTGTTAAAGTTATTCCAAAGAATGAAGCTATGAAATTCACTGCTGGACCGTTTCTTCAAGGAGGATCTTGGCTTCCAACCACTGGTGGACCTTACCTTTTGGGCTTGAAGAACTGAAAAAGAAAAATTGTGTTTGTTGATGAAGAATGTAATATAATTTATTTGATTCCCCCATTCTACAATTATTTATGCCTTAGATCTTAGTTGGTATTTTGTAAGCAATTGCAAATGGCCTTATATGGTCACAACTTTGAGAAGGCGTGCAGGTTAGTAACAAATAAACAACATTTCATTATTACTATTTTTAAACTCTTATAAAAGTAAGATTTATTCTTAATAATAGAGATGTCCATGAGACCAGAAATAGACATTTCTCATTGTCACTTAAATAAAATAAATGGTCACAACTTCTTTAGTTTGGTGGGTATATTTCTCATTGTCACTTGAATAAAATAAATAAATAAATAATCAGATCTCCATTTTTTATATCTCAC

Coding sequence (CDS)

ATGGTGAATAAGGTGGTCTTGCCGGGAATATCCCTTATTCTTGTTGTCGGTGTAGCACTTGCTGTTGTTGCAGTGGTTAACAAGAACAATTCTGACACTTCGACAGAGAATCTTTCCCCAAAAATGAAGGCAGTTTCAACCATTTGTTCTACTACTAATTACCAAGAAGAATGTCAAAATACACTTAGCAATGCTGCTCATAATGCCTCTTCAGATGACCCTAAAGAGTATGTTAAAGCGGCAATTCTTGCAACTATTGAAGAGGTTAAAAAGGGTTATAACTTGACTGATGGCTTTAAGGTTGAGGCTGCTAACAATGCCAGCATCAAAATGGGTGTTGAGGATTGCAGGGACTTATTGCAATTCGCCATTGACCAGTTGCAGGCATCTTACACCACAGTTGGCGAACCCGATTTGCATACCAATGCCGATCGTGTCGCTGATATCAAGAATTGGCTCACTGCTGTCATTTCTTATCAACAATCTTGTCTTGATGGACTTGAAGAATTTGATCCCCAGCTTAAACAGAAGATGCAAGATGGTCTTGATGTTGCTAGCAAACTTACTAGTAATGCTCTTGCTATTGTTGATGCTGTTTCTGACATTCTTGCAAACTTCGGATTGCAATTGAAGGTTCAACCAAGTGGTCGGAGGCTTCTTGGAACAATAGAGGTAGGGAGCGACGGGTTCCCGACATGGATGTCAGGGGCAGACAGGAAGTTGCTAGCATCGAGAGGAGGAGGCGCTCGAGTGAAGCCGAATGCAGTAGTGGCCAAGGACGGTAGCGGACAGTTCAAGACCATTGCTGCTGCTTTGGCTGCATACCCAAAGGTCTTAAGAGGAAGATATGTAATCTACGTAAAAGCAGGGATCTATGATGAATACGTCACTGTAACTAAAGACATGAAAAACATTTTCATGTATGGAGATGGCCCAAAAAAAACCATTGTCACTGGTCGCAAGAGCAACCGTGATGGCTTCACCACCCAAGACACTGCCTCTTTTATAGCCTTAGGAGACGGATTTCTCTGCAAATCAATGGGATTCCGTAACACAGCTGGCCCTGAAGGTCACCAAGCCGTAGCTCTTAGAGTCCAATCTGATAGATCCGCCTTCTTTAATTGCAGAATGGATGGCTACCAAGACACTCTTTACGTTCAAACTCAACGTCAATTCTACCGCAATTGCGTCATCTCCGGCACTGTAGATTTCATCTTCGGCGACTCAACCACCATCATCCAAAACTCCTTGATCATCGTCCGTCGCCCCATGGACAACCAACAGAACACCGTCACGGCCAATGGTCGCGCGGATCCAAAAGAAGTCTCAGGCTTGGTGATTCACAACTGCAGAATCGTGCCAGAACAGAAGCTGTTTGCTGAGAGGTTCAAGATTCCTACATACTTGGGACGGCCATGGAAGCAATATGCTAGAACTGTGATCATGGAGTCGACATTGGGTGACTTCATTAAGCCAGACGGTTACATGCCATGGTCTGGAAATTTTGCATTGGAAACTTGTTTGTATTTGGAGTATGGAAACAGAGGACCTGGGGCTAACACTAACAGGAGAGTAAGATGGAAGGGTGTTAAAGTTATTCCAAAGAATGAAGCTATGAAATTCACTGCTGGACCGTTTCTTCAAGGAGGATCTTGGCTTCCAACCACTGGTGGACCTTACCTTTTGGGCTTGAAGAACTGA

Protein sequence

MVNKVVLPGISLILVVGVALAVVAVVNKNNSDTSTENLSPKMKAVSTICSTTNYQEECQNTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDLLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADRKLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLLGLKN
Homology
BLAST of Lsi09G007590 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 455.3 bits (1170), Expect = 1.0e-126
Identity = 242/524 (46.18%), Postives = 334/524 (63.74%), Query Frame = 0

Query: 43  KAVSTICSTTNYQEECQNTLSNAAHNA-SSDDPKEYVKAAILATIEEVKKGYNLTDGFKV 102
           K + T+CS+T Y + C+ TL N      + D+P  ++K+AI A  E++          K 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 103 EAANNASIKMGVEDCRDLLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQ 162
           E   N   K  +E C+ L++ A ++  AS   +   ++++    V D+++WL+AV+SYQ+
Sbjct: 167 E---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 163 SCLDGLEEFDPQLKQKMQDGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLG 222
           +CLDG EE    LK +++  ++ +  LTSN+LA++   ++   N    +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTE---NLSPVMKVVE--RHLL- 286

Query: 223 TIEVGSDGFPTWMSGADRKLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRG 282
                 D  P+W+S  DR++L +    A +KPNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 283 RYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDG 342
           RY+IYVK GIYDEYVTV K   N+ M GDG +KTIVTG KS+     T  TA+F+A G+G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 343 FLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 402
           F+ +SMGFRNTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  T RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 403 VDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 462
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 463 RFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPW-SGNFALETCLYLEYGNRGPGAN 522
           + +  +YLGRPWK Y+RT+IMES + + I P G++ W   +FA++T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 523 TNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLLGL 565
           T  RV+W G KVI K EA+ +T GPFLQ G W+  +G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQ-GDWISASGSPVKLGL 611

BLAST of Lsi09G007590 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 1.0e-126
Identity = 232/566 (40.99%), Postives = 349/566 (61.66%), Query Frame = 0

Query: 4   KVVLPGISLILVVGVALAVVAVVNKNNSDTS-TENLSPKMKAVSTICSTTNYQEECQNTL 63
           + V+  IS +L++ + +AV   V+ N SD +  E ++  +KA+  +C+ T+Y+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVKAAILATIEEV----KKGYNLTDGFKVEAANNASIKMGVEDCRD 123
              A + S  DP E VK A  AT++++    KK   +     +E   +   KM ++ C++
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTM-----IELQKDPRAKMALDQCKE 134

Query: 124 LLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 183
           L+ +AI +L  S+  +G+ + H   + +  ++ WL+A IS++Q+CLDG +       + +
Sbjct: 135 LMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETI 194

Query: 184 QDGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGAD 243
           +  L  A +LT N LA+V  +S+ L    +    + + RRLL      S  FP+WM    
Sbjct: 195 KKALKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARA 254

Query: 244 RKLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTV 303
           R+LL      + VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++K GIY EYV V
Sbjct: 255 RRLL--NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQV 314

Query: 304 TKDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGH 363
            + M ++   GDGP KT+++G KS +DG TT  TA+   +GD F+ K++ F NTAG   H
Sbjct: 315 NRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKH 374

Query: 364 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 423
           QAVA+RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  + QN  +
Sbjct: 375 QAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTL 434

Query: 424 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYAR 483
           +VR+P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  TYLGRPWK+Y+R
Sbjct: 435 LVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSR 494

Query: 484 TVIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEA 543
           T+IM + + DF+ P+G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E 
Sbjct: 495 TIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEI 554

Query: 544 MKFTAGPFLQGGSWLPTTGGPYLLGL 565
           +KFT   ++QG +W+P  G PY+LGL
Sbjct: 555 LKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Lsi09G007590 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.3e-126
Identity = 263/602 (43.69%), Postives = 358/602 (59.47%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNSDTSTENLSPKMKAVSTICSTTNYQEECQN 60
           M+ KVV+   S++L+VGVA+ VVA +NKN       NLSP+MKAV  IC +T+ +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGD----ANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNAS--IKMGVEDCRD 120
           TL        S+DP + +KA +LAT +E+ K  N T   +V   ++ S   K  ++ C+ 
Sbjct: 61  TL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVASKLTSNALAIVDAVSDILANFGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V   +A    ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTIEVGSDGFPTWMSGADRKLLA--------SRGGGARVKPNAVVAKDGSGQF 300
           G  RRLL  I+    G PTW+SGADRKL+A         RGGGARV+ N VVAKDGSGQF
Sbjct: 241 GPARRLLEDID--ETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKS--N 360
           KT+  A+ A P+  RGR +IY+KAG+Y E V + K   NIFM+GDG +KT+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 420
             G TT  +A+     +GF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVS 480
           LYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      + 
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 -GLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPWSGNF 540
            G+V+ NCRIVP++KL  ER  + TYLGRPWK+++ TVIM + +GD I+P+G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLL 565
             ++C Y+EY NRGPGA  NRRV W  V      E   FTA  +L   +W+     P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of Lsi09G007590 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 451.1 bits (1159), Expect = 1.9e-125
Identity = 224/559 (40.07%), Postives = 347/559 (62.08%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAVVNKNNSDTSTE---NLSPKMKAVSTICSTTNYQEECQNTLSNAA 69
           IS +L++ + +AV   V+ N  D  ++    ++  +KAV  +C+ T+Y++ C++TL    
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNG 79

Query: 70  HNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDLLQFAIDQ 129
            N +  DP E VK A   T++++      +    +E   ++  +M ++ C++L+ +A+D+
Sbjct: 80  KNTT--DPMELVKTAFNVTMKQITDAAKKSQTI-MELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAS 189
           L  S+  +G+ + H   + + +++ WL+A IS++++CL+G +       + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDAVSDILANFGLQLKVQP-SGRRLLGTIEVGSDGFPTWMSGADRKLLASR 249
           +LT N LAI+  +S    NF  Q+++   + RRLL      ++GFP+W+    RKLL + 
Sbjct: 200 ELTHNGLAIISEMS----NFVGQMQIPGLNSRRLL------AEGFPSWVDQRGRKLLQAA 259

Query: 250 GGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNI 309
              + VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EYV V K M ++
Sbjct: 260 AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHL 319

Query: 310 FMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRV 369
              GDGP KTI++G K+ +DG TT  TA+   +G+ F+ K++GF NTAG   HQAVA+RV
Sbjct: 320 VFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRV 379

Query: 370 QSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMD 429
           QSD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  + QN  ++VR+P+ 
Sbjct: 380 QSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLP 439

Query: 430 NQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMEST 489
           NQ   +TA+GR DP+E +G V   C I  E    A +     YLGRPWK+Y+RT+IM + 
Sbjct: 440 NQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTF 499

Query: 490 LGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGP 549
           + DF++P G+ PW G+F L+T  Y E  N GPG+    RV W G+K + + + +KFT   
Sbjct: 500 IPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQ 559

Query: 550 FLQGGSWLPTTGGPYLLGL 565
           ++QG  W+P  G PY  GL
Sbjct: 560 YIQGDDWIPGKGVPYTTGL 565

BLAST of Lsi09G007590 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.9e-125
Identity = 254/598 (42.47%), Postives = 361/598 (60.37%), Query Frame = 0

Query: 4   KVVLPGISLILV----VGVALAVVAVVNK------------NNSDTSTENLSPKM----- 63
           +++L  +S+++V    +G A A V   NK             +S T +E+ SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVSTICSTTNYQEECQNTLSN-AAHNASSDDPKEYVKAAILATIEEVKKG 123
                    K + T+C++T Y+  CQNTL N    +    DP+  +K+AI+A  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDGFKVEAANNASIKMGVEDCRDLLQFAIDQLQASYTTVGEPDLHTNADRVADIKNW 183
           +      K E   N   K  +  C+ L+  A ++L  S   + + +++  A  V D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVASKLTSNALAIVDAVSDILANF-----G 243
           L+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L++       
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTR 263

Query: 244 LQLKVQPSGRRLLGTIEVGSDGFPTWMSGADRKLLASRGGGARVKPNAVVAKDGSGQFKT 303
           L L+ + S +         +D   +W+S  +R++L +    A +KPNA VAKDGSG F T
Sbjct: 264 LLLEARSSAKE--------TDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTT 323

Query: 304 IAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKSNRDGF 363
           I AAL A P   +GRY IY+K GIYDE V + K   N+ M GDG +KTIVTG KS+    
Sbjct: 324 INAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKI 383

Query: 364 TTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQ 423
            T  TA+F+A G+GF+ +SMGFRNTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  
Sbjct: 384 RTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAY 443

Query: 424 TQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVI 483
           T RQ+YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G VI
Sbjct: 444 THRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVI 503

Query: 484 HNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPW-SGNFALET 543
           HNC + P + L   + +  +YLGRPWK ++RTV+MEST+ D I P G++ W   +FA++T
Sbjct: 504 HNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDT 563

Query: 544 CLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLLGL 565
             Y EY N GP   T  RV+W G +V+ K EAMKFT GPFLQ G W+   G P  LGL
Sbjct: 564 LSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQ-GEWIQAIGSPVKLGL 606

BLAST of Lsi09G007590 vs. ExPASy TrEMBL
Match: A0A5A7U9J8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100 PE=3 SV=1)

HSP 1 Score: 1042.7 bits (2695), Expect = 5.5e-301
Identity = 515/567 (90.83%), Postives = 546/567 (96.30%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKN-NSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MVNKV+LPGISLILVVGVALAVVA+V+ N +SD +TE+LSPKMKAVSTIC+  NYQ+ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGF +EAANN SIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVDAVSDILA+FGLQLK QPSGRRLLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLASRGGGA+VKPNAVVAKDGSGQFKTI+AALAAYPK LRGRYVIYVKAGIY+EYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTG KSNR GFTTQDTA+FIA+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI +NEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           +FTAGPFLQG +WLPTTGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lsi09G007590 vs. ExPASy TrEMBL
Match: A0A1S3CR27 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.2e-300
Identity = 514/567 (90.65%), Postives = 546/567 (96.30%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKN-NSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MVNKV+LPGI+LILVVGVALAVVA+V+ N +SD +TE+LSPKMKAVSTIC+  NYQ+ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGF +EAANN SIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVDAVSDILA+FGLQLK QPSGRRLLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLASRGGGA+VKPNAVVAKDGSGQFKTI+AALAAYPK LRGRYVIYVKAGIY+EYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTG KSNR GFTTQDTA+FIA+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI +NEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           +FTAGPFLQG +WLPTTGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lsi09G007590 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.9e-277
Identity = 472/567 (83.25%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNK-NNSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MV KV++  +SLILVVG+ALAVVA +NK N S+   E +SPKMKA+S ICSTT+YQEEC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTL++ AHN SS+DPK+Y KAAILATI+E+ KGYNL+D   VEA NNASIKM VEDC+DL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAID+LQASY+TVGE DLHT+ADR+ADIKNWL+AV+SYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGLDVA KLTSNALAIV AVS+IL N+GLQLKVQPSGRRLLGT EV SDGFPTW++GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLAS+ GG R  PNAVVAKDGSGQ+KTIAAALAAYPK LRGRYVIYVKAGIYDEY+TVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTGRKSNRDGFTTQ+TASF A+G+GFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI +NEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           ++TAG FL G +WLP TGG Y LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007590 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 7.2e-277
Identity = 471/566 (83.22%), Postives = 515/566 (90.99%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNSDTSTENLSPKMKAVSTICSTTNYQEECQN 60
           MV KVV+ GISLILVVGVALAVVA+VNK++S   T NLSPKMKAV++ICS  +YQ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDLL 120
           TL +  +N+S +DPKE++KAAI +TIEE+KKGYNLTD   VEAANNA+IKM V+DC+DLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           Q AID+L ASY+TVG+PDLHTN DR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADRK 240
           GLDVA KLTSNALAIV AVSDIL   GLQLKVQPSGRRLLGT EV SD FPTW++G+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTK 300
           LLA++ GG RVKPN VVAKDGSGQ+KTI AALAAYPK L+GRYVIYVKAG+Y+E + VTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQA 360
           DMKNIFMYGDGP+KTIVTGRKSNRDG TTQ+TASF A+G+GFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPT+LGRPWK+YARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMK 540
           IMESTLGDFI+P GYMPWSGNFAL TC Y EYGNRGPGANTNRRVRWKGV+VI +NEAM+
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLQGGSWLPTTGGPYLLGLKN 567
           FTAGPFL G +WLP TGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Lsi09G007590 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.1e-276
Identity = 470/567 (82.89%), Postives = 520/567 (91.71%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNK-NNSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MV KV++  +SLILVVG+ALAVVA +NK N S+   E +SPKMKA+S ICSTT+YQEEC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTL++ A N SS+DPK+Y KAAILATI+E+ KGYNL+D   VEA NNASIKM VEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAID+LQASY+TVGE DLHT+ADR+ADIKNWL+AV+SYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGLDVA KLTSNALAI+ AVS+IL N+GLQLKVQPSGRRLLGT EV SDGFPTW++GADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLAS+ GG R  PNAVVAKDGSGQ+KTIAAALAAYPK LRGRYVIYVKAGIYDEY+TVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTGRKSNRDGFTTQ+TASF A+G+GFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI +NEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           ++TAG FL G +WLP TGG Y LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007590 vs. NCBI nr
Match: XP_038875878.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 1052.4 bits (2720), Expect = 1.4e-303
Identity = 523/565 (92.57%), Postives = 545/565 (96.46%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNSDTSTENLSPKMKAVSTICSTTNYQEECQN 60
           MVNKVVLPGISLILVVGVALAVVAVVNKNN+ T TENLSPKMKAVSTICS TNYQEECQN
Sbjct: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNNST-TENLSPKMKAVSTICSNTNYQEECQN 60

Query: 61  TLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDLL 120
           TL+N AHNASSDDPKEYVKAAILAT+EEVKKGYNLTDGF +EAANNASIKMGVEDC+DLL
Sbjct: 61  TLTNVAHNASSDDPKEYVKAAILATMEEVKKGYNLTDGFMIEAANNASIKMGVEDCKDLL 120

Query: 121 QFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QFAI QLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGL EFDP L+QKMQD
Sbjct: 121 QFAIAQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLGEFDPNLQQKMQD 180

Query: 181 GLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADRK 240
           GLD+A+KLTSNALAIVD VSDIL NFGLQ KVQPSGR+LLGTIEVGSDGFPTWMSGADRK
Sbjct: 181 GLDIAAKLTSNALAIVDTVSDILQNFGLQFKVQPSGRKLLGTIEVGSDGFPTWMSGADRK 240

Query: 241 LLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTK 300
           LLASRGGG RVKPNAVVAKDGSGQFKTI AALAAYPK L+GRYVIYVKAGIY+EYVTVTK
Sbjct: 241 LLASRGGG-RVKPNAVVAKDGSGQFKTIGAALAAYPKALKGRYVIYVKAGIYNEYVTVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQA 360
           DM NIFMYGDGPK+TIVTGR+SNR G+TTQDTA+FIA+G GFLCKSMGF+NTAGPEGHQA
Sbjct: 301 DMTNIFMYGDGPKRTIVTGRRSNRAGYTTQDTATFIAIGAGFLCKSMGFQNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420
           VALRVQSD+SAFFNCRMDGYQDTLYVQT+RQFYRNCVISGTVDFIFGDSTTIIQNSLIIV
Sbjct: 361 VALRVQSDKSAFFNCRMDGYQDTLYVQTERQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480
           RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480

Query: 481 IMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMK 540
           IME+TLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIP+ EA+K
Sbjct: 481 IMETTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPRTEALK 540

Query: 541 FTAGPFLQGGSWLPTTGGPYLLGLK 566
           FTAG FLQGGSWLP TGGPYLLGLK
Sbjct: 541 FTAGSFLQGGSWLPGTGGPYLLGLK 563

BLAST of Lsi09G007590 vs. NCBI nr
Match: KAA0050275.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.1e-300
Identity = 515/567 (90.83%), Postives = 546/567 (96.30%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKN-NSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MVNKV+LPGISLILVVGVALAVVA+V+ N +SD +TE+LSPKMKAVSTIC+  NYQ+ECQ
Sbjct: 1   MVNKVILPGISLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGF +EAANN SIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVDAVSDILA+FGLQLK QPSGRRLLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLASRGGGA+VKPNAVVAKDGSGQFKTI+AALAAYPK LRGRYVIYVKAGIY+EYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTG KSNR GFTTQDTA+FIA+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI +NEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           +FTAGPFLQG +WLPTTGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lsi09G007590 vs. NCBI nr
Match: XP_008466374.1 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1041.6 bits (2692), Expect = 2.5e-300
Identity = 514/567 (90.65%), Postives = 546/567 (96.30%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKN-NSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MVNKV+LPGI+LILVVGVALAVVA+V+ N +SD +TE+LSPKMKAVSTIC+  NYQ+ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTLSNAAHNASSDDPKEYV+AAIL+TIEEVKKGYNLTDGF +EAANN SIKMGVEDCRDL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAIDQLQASY+TVGEPDLHTNADRVADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGL  A KLTSNALAIVDAVSDILA+FGLQLK QPSGRRLLGT EVG DG+PTW++GADR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLASRGGGA+VKPNAVVAKDGSGQFKTI+AALAAYPK LRGRYVIYVKAGIY+EYV +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKNIFMYGDGPKKTIVTG KSNR GFTTQDTA+FIA+G+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVI +NEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           +FTAGPFLQG +WLPTTGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Lsi09G007590 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 993.0 bits (2566), Expect = 1.0e-285
Identity = 488/566 (86.22%), Postives = 528/566 (93.29%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNSDTSTENLSPKMKAVSTICSTTNYQEECQN 60
           MV KVV+ GISLILVVGVALAVVA+V KNN ++  +  SPKMKAVSTICSTT+YQ+ECQN
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNN-NSDVDLSSPKMKAVSTICSTTDYQQECQN 60

Query: 61  TLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDLL 120
           TL+N A NAS DDPKEY KAA+LATI+E+ KGYNLT    VEAANNASIKM V+DC+DLL
Sbjct: 61  TLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLL 120

Query: 121 QFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           Q AID+L ASY+TVG+PDLHTN DRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADRK 240
           GLDVA KLTSNALAIV AVSDIL NFGLQLKVQPSGRRLL T E+GSDGFPTWMSGADRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRK 240

Query: 241 LLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTK 300
           LLASRGGGARVKPNAVVAKDGSGQ+K+I AALAAYPK LRGRYVIYVKAGIYDEY+TVTK
Sbjct: 241 LLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTK 300

Query: 301 DMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQA 360
           DMKN+FMYGDGP+KTIVTGRK+NRDG TTQ+TASF A+G+GFLCKSMGFRNTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420
           VALRVQSDRSAF+NCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTV 480
           RRPMDNQ+NTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYARTV
Sbjct: 421 RRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 480

Query: 481 IMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMK 540
           IME+TLGDFI+PDGYMPWSGNFALETC Y EYGNRGPGANTNRRVRWKGV+VI +NEA++
Sbjct: 481 IMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQ 540

Query: 541 FTAGPFLQGGSWLPTTGGPYLLGLKN 567
           +TAGPFL G +WLP TGGPYLLGLK+
Sbjct: 541 YTAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of Lsi09G007590 vs. NCBI nr
Match: KAA0050274.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 964.5 bits (2492), Expect = 3.9e-277
Identity = 472/567 (83.25%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNK-NNSDTSTENLSPKMKAVSTICSTTNYQEECQ 60
           MV KV++  +SLILVVG+ALAVVA +NK N S+   E +SPKMKA+S ICSTT+YQEEC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDL 120
           NTL++ AHN SS+DPK+Y KAAILATI+E+ KGYNL+D   VEA NNASIKM VEDC+DL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQFAID+LQASY+TVGE DLHT+ADR+ADIKNWL+AV+SYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGADR 240
           DGLDVA KLTSNALAIV AVS+IL N+GLQLKVQPSGRRLLGT EV SDGFPTW++GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVT 300
           KLLAS+ GG R  PNAVVAKDGSGQ+KTIAAALAAYPK LRGRYVIYVKAGIYDEY+TVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTGRKSNRDGFTTQ+TASF A+G+GFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAERFKIPT+LGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAM 540
           VIMESTLGDFI+P GYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI +NEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 KFTAGPFLQGGSWLPTTGGPYLLGLKN 567
           ++TAG FL G +WLP TGG Y LGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Lsi09G007590 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 455.3 bits (1170), Expect = 7.2e-128
Identity = 242/524 (46.18%), Postives = 334/524 (63.74%), Query Frame = 0

Query: 43  KAVSTICSTTNYQEECQNTLSNAAHNA-SSDDPKEYVKAAILATIEEVKKGYNLTDGFKV 102
           K + T+CS+T Y + C+ TL N      + D+P  ++K+AI A  E++          K 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 103 EAANNASIKMGVEDCRDLLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQ 162
           E   N   K  +E C+ L++ A ++  AS   +   ++++    V D+++WL+AV+SYQ+
Sbjct: 167 E---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 163 SCLDGLEEFDPQLKQKMQDGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLG 222
           +CLDG EE    LK +++  ++ +  LTSN+LA++   ++   N    +KV    R LL 
Sbjct: 227 TCLDGFEE--GNLKSEVKTSVNSSQVLTSNSLALIKTFTE---NLSPVMKVVE--RHLL- 286

Query: 223 TIEVGSDGFPTWMSGADRKLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRG 282
                 D  P+W+S  DR++L +    A +KPNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKA-LKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 283 RYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDG 342
           RY+IYVK GIYDEYVTV K   N+ M GDG +KTIVTG KS+     T  TA+F+A G+G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 343 FLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGT 402
           F+ +SMGFRNTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  T RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 403 VDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAE 462
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 463 RFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPW-SGNFALETCLYLEYGNRGPGAN 522
           + +  +YLGRPWK Y+RT+IMES + + I P G++ W   +FA++T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 523 TNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLLGL 565
           T  RV+W G KVI K EA+ +T GPFLQ G W+  +G P  LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQ-GDWISASGSPVKLGL 611

BLAST of Lsi09G007590 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 455.3 bits (1170), Expect = 7.2e-128
Identity = 232/566 (40.99%), Postives = 349/566 (61.66%), Query Frame = 0

Query: 4   KVVLPGISLILVVGVALAVVAVVNKNNSDTS-TENLSPKMKAVSTICSTTNYQEECQNTL 63
           + V+  IS +L++ + +AV   V+ N SD +  E ++  +KA+  +C+ T+Y+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  SNAAHNASSDDPKEYVKAAILATIEEV----KKGYNLTDGFKVEAANNASIKMGVEDCRD 123
              A + S  DP E VK A  AT++++    KK   +     +E   +   KM ++ C++
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTM-----IELQKDPRAKMALDQCKE 134

Query: 124 LLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 183
           L+ +AI +L  S+  +G+ + H   + +  ++ WL+A IS++Q+CLDG +       + +
Sbjct: 135 LMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETI 194

Query: 184 QDGLDVASKLTSNALAIVDAVSDILANFGLQLKVQPSGRRLLGTIEVGSDGFPTWMSGAD 243
           +  L  A +LT N LA+V  +S+ L    +    + + RRLL      S  FP+WM    
Sbjct: 195 KKALKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARA 254

Query: 244 RKLLASRGGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTV 303
           R+LL      + VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++K GIY EYV V
Sbjct: 255 RRLL--NAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQV 314

Query: 304 TKDMKNIFMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGH 363
            + M ++   GDGP KT+++G KS +DG TT  TA+   +GD F+ K++ F NTAG   H
Sbjct: 315 NRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKH 374

Query: 364 QAVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLI 423
           QAVA+RV +D S F+NC+ DGYQDTLY  + RQFYR+C ISGT+DF+FGD+  + QN  +
Sbjct: 375 QAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTL 434

Query: 424 IVRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYAR 483
           +VR+P+ NQ   +TA+GR DP+E +G V+  C IV E    A + +  TYLGRPWK+Y+R
Sbjct: 435 LVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSR 494

Query: 484 TVIMESTLGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEA 543
           T+IM + + DF+ P+G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E 
Sbjct: 495 TIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEI 554

Query: 544 MKFTAGPFLQGGSWLPTTGGPYLLGL 565
           +KFT   ++QG +W+P  G PY+LGL
Sbjct: 555 LKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Lsi09G007590 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 454.9 bits (1169), Expect = 9.4e-128
Identity = 263/602 (43.69%), Postives = 358/602 (59.47%), Query Frame = 0

Query: 1   MVNKVVLPGISLILVVGVALAVVAVVNKNNSDTSTENLSPKMKAVSTICSTTNYQEECQN 60
           M+ KVV+   S++L+VGVA+ VVA +NKN       NLSP+MKAV  IC +T+ +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGD----ANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLSNAAHNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNAS--IKMGVEDCRD 120
           TL        S+DP + +KA +LAT +E+ K  N T   +V   ++ S   K  ++ C+ 
Sbjct: 61  TL----EPVKSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQFAIDQLQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           +  +A++ L      +GE DL     ++  +K WL  V +YQ  CLD +EE D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVASKLTSNALAIVDAVSDILANFGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V   +A    ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTIEVGSDGFPTWMSGADRKLLA--------SRGGGARVKPNAVVAKDGSGQF 300
           G  RRLL  I+    G PTW+SGADRKL+A         RGGGARV+ N VVAKDGSGQF
Sbjct: 241 GPARRLLEDID--ETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKS--N 360
           KT+  A+ A P+  RGR +IY+KAG+Y E V + K   NIFM+GDG +KT+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 420
             G TT  +A+     +GF+ K MGF+NTAGP GHQA A+RV  DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVS 480
           LYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      + 
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 -GLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPWSGNF 540
            G+V+ NCRIVP++KL  ER  + TYLGRPWK+++ TVIM + +GD I+P+G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLL 565
             ++C Y+EY NRGPGA  NRRV W  V      E   FTA  +L   +W+     P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of Lsi09G007590 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-126
Identity = 224/559 (40.07%), Postives = 347/559 (62.08%), Query Frame = 0

Query: 10  ISLILVVGVALAVVAVVNKNNSDTSTE---NLSPKMKAVSTICSTTNYQEECQNTLSNAA 69
           IS +L++ + +AV   V+ N  D  ++    ++  +KAV  +C+ T+Y++ C++TL    
Sbjct: 20  ISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNG 79

Query: 70  HNASSDDPKEYVKAAILATIEEVKKGYNLTDGFKVEAANNASIKMGVEDCRDLLQFAIDQ 129
            N +  DP E VK A   T++++      +    +E   ++  +M ++ C++L+ +A+D+
Sbjct: 80  KNTT--DPMELVKTAFNVTMKQITDAAKKSQTI-MELQKDSRTRMALDQCKELMDYALDE 139

Query: 130 LQASYTTVGEPDLHTNADRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAS 189
           L  S+  +G+ + H   + + +++ WL+A IS++++CL+G +       + M+  L  A 
Sbjct: 140 LSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALKTAI 199

Query: 190 KLTSNALAIVDAVSDILANFGLQLKVQP-SGRRLLGTIEVGSDGFPTWMSGADRKLLASR 249
           +LT N LAI+  +S    NF  Q+++   + RRLL      ++GFP+W+    RKLL + 
Sbjct: 200 ELTHNGLAIISEMS----NFVGQMQIPGLNSRRLL------AEGFPSWVDQRGRKLLQAA 259

Query: 250 GGGARVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNI 309
              + VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EYV V K M ++
Sbjct: 260 AAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHL 319

Query: 310 FMYGDGPKKTIVTGRKSNRDGFTTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRV 369
              GDGP KTI++G K+ +DG TT  TA+   +G+ F+ K++GF NTAG   HQAVA+RV
Sbjct: 320 VFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRV 379

Query: 370 QSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMD 429
           QSD S FFNCR DGYQDTLY  + RQF+R+C ISGT+DF+FGD+  + QN  ++VR+P+ 
Sbjct: 380 QSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLP 439

Query: 430 NQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMEST 489
           NQ   +TA+GR DP+E +G V   C I  E    A +     YLGRPWK+Y+RT+IM + 
Sbjct: 440 NQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTF 499

Query: 490 LGDFIKPDGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGP 549
           + DF++P G+ PW G+F L+T  Y E  N GPG+    RV W G+K + + + +KFT   
Sbjct: 500 IPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTPAQ 559

Query: 550 FLQGGSWLPTTGGPYLLGL 565
           ++QG  W+P  G PY  GL
Sbjct: 560 YIQGDDWIPGKGVPYTTGL 565

BLAST of Lsi09G007590 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-126
Identity = 254/598 (42.47%), Postives = 361/598 (60.37%), Query Frame = 0

Query: 4   KVVLPGISLILV----VGVALAVVAVVNK------------NNSDTSTENLSPKM----- 63
           +++L  +S+++V    +G A A V   NK             +S T +E+ SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVSTICSTTNYQEECQNTLSN-AAHNASSDDPKEYVKAAILATIEEVKKG 123
                    K + T+C++T Y+  CQNTL N    +    DP+  +K+AI+A  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDGFKVEAANNASIKMGVEDCRDLLQFAIDQLQASYTTVGEPDLHTNADRVADIKNW 183
           +      K E   N   K  +  C+ L+  A ++L  S   + + +++  A  V D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVASKLTSNALAIVDAVSDILANF-----G 243
           L+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L++       
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTR 263

Query: 244 LQLKVQPSGRRLLGTIEVGSDGFPTWMSGADRKLLASRGGGARVKPNAVVAKDGSGQFKT 303
           L L+ + S +         +D   +W+S  +R++L +    A +KPNA VAKDGSG F T
Sbjct: 264 LLLEARSSAKE--------TDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTT 323

Query: 304 IAAALAAYPKVLRGRYVIYVKAGIYDEYVTVTKDMKNIFMYGDGPKKTIVTGRKSNRDGF 363
           I AAL A P   +GRY IY+K GIYDE V + K   N+ M GDG +KTIVTG KS+    
Sbjct: 324 INAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKI 383

Query: 364 TTQDTASFIALGDGFLCKSMGFRNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQ 423
            T  TA+F+A G+GF+ +SMGFRNTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  
Sbjct: 384 RTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAY 443

Query: 424 TQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIVRRPMDNQQNTVTANGRADPKEVSGLVI 483
           T RQ+YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR D  + +G VI
Sbjct: 444 THRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVI 503

Query: 484 HNCRIVPEQKLFAERFKIPTYLGRPWKQYARTVIMESTLGDFIKPDGYMPW-SGNFALET 543
           HNC + P + L   + +  +YLGRPWK ++RTV+MEST+ D I P G++ W   +FA++T
Sbjct: 504 HNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDT 563

Query: 544 CLYLEYGNRGPGANTNRRVRWKGVKVIPKNEAMKFTAGPFLQGGSWLPTTGGPYLLGL 565
             Y EY N GP   T  RV+W G +V+ K EAMKFT GPFLQ G W+   G P  LGL
Sbjct: 564 LSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQ-GEWIQAIGSPVKLGL 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y2011.0e-12646.18Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z81.0e-12640.99Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
O807221.3e-12643.69Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q8GX861.9e-12540.07Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY61.9e-12542.47Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7U9J85.5e-30190.83Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00100... [more]
A0A1S3CR271.2e-30090.65Pectinesterase OS=Cucumis melo OX=3656 GN=LOC107990290 PE=3 SV=1[more]
A0A5A7U4V71.9e-27783.25Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110... [more]
A0A0A0KLN27.2e-27783.22Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A1S3CSH26.1e-27682.89Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875878.11.4e-30392.57pectinesterase-like [Benincasa hispida][more]
KAA0050275.11.1e-30090.83pectinesterase-like [Cucumis melo var. makuwa] >TYJ98132.1 pectinesterase-like [... [more]
XP_008466374.12.5e-30090.65PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038875879.11.0e-28586.22LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
KAA0050274.13.9e-27783.25pectinesterase-like [Cucumis melo var. makuwa] >TYJ98133.1 pectinesterase-like [... [more]
Match NameE-valueIdentityDescription
AT2G26450.17.2e-12846.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.17.2e-12840.99Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.19.4e-12843.69Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.11.4e-12640.07Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.4e-12642.47Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..195
e-value: 2.0E-34
score: 130.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 22..197
e-value: 4.2E-19
score: 66.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 44..195
e-value: 1.2E-24
score: 87.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 248..564
e-value: 2.9E-111
score: 373.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 42..200
e-value: 4.9E-30
score: 106.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 43..199
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 254..549
e-value: 5.4E-131
score: 435.7
NoneNo IPR availablePANTHERPTHR31707:SF164PECTINESTERASE 4-RELATEDcoord: 4..564
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 4..564
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..200
e-value: 2.93912E-49
score: 165.689
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 398..407
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 251..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G007590.1Lsi09G007590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity