Lsi09G007520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G007520
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDUF295 domain-containing protein
Locationchr09: 8635446 .. 8636756 (-)
RNA-Seq ExpressionLsi09G007520
SyntenyLsi09G007520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGACGAGGGAGTGCGGTGGTCGGAACTTCCGCCGGAGATCTAGTCAGCAGTCGGAAAGCACCTCCACAATTACATCGACGCCGTCCGATTTCGCAGCGTTTGCCGATCATGGCGGTCCTCTATTCTTCCTTTCCCCCAAACATCTCCTGCTCTTCGTCTTCGATTTCCTTCTCCATTCATCACTGCAAATCTCTTCGTCGATGCCTTCCTCTCCCAAAGCACACTCTATCGCCTCGCTCCTCTCGACCACGAACCAAGCTCTAACGTTTCTTCTTCGTCTTCCCCTCGCCGGAATGGCCGTCTGGTGAAGCTGGAGAAATCGGAGCTAGGTAAGATGCGATTTCTTCATCCTTTGTCCAGACGCCCAATCCGATGCAACCTGGAAGATCTTTCGGAAGAACACAATGAATTGAACTTACTGGATCTTCAAATCGACGAATTAGTTATATCCTATTCGCTCAAGTACACTGATACCACCTGTGTTCATGGAATCGCTAAAATCGTTGTATTCCCAAATTCCACCGGACCGGATGTTAAAGATTGTACCGTAATCGCAGTGTTTGAGAACGGGAAGTTAGGGTTTGCCAGATCTGGTGACGAGAAATGGACTCTAATTGACGAACAAAATTTCCACTACGACGATGTAATTGTCTACAATCAGCAATACTACACAGTCGATAGACGGGGAACAGTTTTCTGGATCGATTCATCGATGCGATTGGTACAATTTTCTCCTTCTCTGGTCGGTCTCGGCCACCATAAGCACTTAGTAGAGTGCGGCGGCGAACTTCTAGTAATCGATCGATTCCTCGACAAAGAGCGTCAGTTTCAACACCCAATCGCCGTCGTTGACGACGCACAACATCCTTTCGCGATGCCAGTTCGACGGCCGCCTTACGATTCGTCAGCAAGAGCAGTGGATTTCAAAGTTCATAAGCTGGATCAAGAATGGGGGACATGGGTGGAGCTGAAGAACTTGAGAAATCGATCGATTATATTGGGGAACGATTGTTGTTTCTCAGTGGAAGCATCGGAGTTTGAAGGATGTAAAGAGAATTGCATTTACTATACGGATCTGGACGACGGGGAATTCGTTCAAAGAAGTACGAGTCGTGTGTTTGATCTTGAAGGAAGAAGGATCGGTAAGATACTGTGTTATCCTGGACGAATCGGGATTTTCAACCCGCCGCCGATTTGGCTCTCAAAATCTCTTCGTCCATTGAAGGTACGTATACATATGAAATCTGGGTTTCTTGAGAATTATGTGATCAAACTGGGATTTCAACTTATTTCTATTGGATCTTAA

mRNA sequence

ATGGACGACGAGGGAGTGCGGTGCGTTTGCCGATCATGGCGGTCCTCTATTCTTCCTTTCCCCCAAACATCTCCTGCTCTTCGTCTTCGATTTCCTTCTCCATTCATCACTGCAAATCTCTTCGTCGATGCCTTCCTCTCCCAAAGCACACTCTATCGCCTCGCTCCTCTCGACCACGAACCAAGCTCTAACGTTTCTTCTTCGTCTTCCCCTCGCCGGAATGGCCGTCTGGTGAAGCTGGAGAAATCGGAGCTAGGTAAGATGCGATTTCTTCATCCTTTGTCCAGACGCCCAATCCGATGCAACCTGGAAGATCTTTCGGAAGAACACAATGAATTGAACTTACTGGATCTTCAAATCGACGAATTAGTTATATCCTATTCGCTCAAGTACACTGATACCACCTGTGTTCATGGAATCGCTAAAATCGTTGTATTCCCAAATTCCACCGGACCGGATGTTAAAGATTGTACCGTAATCGCAGTGTTTGAGAACGGGAAGTTAGGGTTTGCCAGATCTGGTGACGAGAAATGGACTCTAATTGACGAACAAAATTTCCACTACGACGATGTAATTGTCTACAATCAGCAATACTACACAGTCGATAGACGGGGAACAGTTTTCTGGATCGATTCATCGATGCGATTGGTACAATTTTCTCCTTCTCTGGTCGGTCTCGGCCACCATAAGCACTTAGTAGAGTGCGGCGGCGAACTTCTAGTAATCGATCGATTCCTCGACAAAGAGCGTCAGTTTCAACACCCAATCGCCGTCGTTGACGACGCACAACATCCTTTCGCGATGCCAGTTCGACGGCCGCCTTACGATTCGTCAGCAAGAGCAGTGGATTTCAAAGTTCATAAGCTGGATCAAGAATGGGGGACATGGGTGGAGCTGAAGAACTTGAGAAATCGATCGATTATATTGGGGAACGATTGTTGTTTCTCAGTGGAAGCATCGGAGTTTGAAGGATGTAAAGAGAATTGCATTTACTATACGGATCTGGACGACGGGGAATTCGTTCAAAGAAGTACGAGTCGTGTGTTTGATCTTGAAGGAAGAAGGATCGGTAAGATACTGTGTTATCCTGGACGAATCGGGATTTTCAACCCGCCGCCGATTTGGCTCTCAAAATCTCTTCGTCCATTGAAGGTACGTATACATATGAAATCTGGGTTTCTTGAGAATTATGTGATCAAACTGGGATTTCAACTTATTTCTATTGGATCTTAA

Coding sequence (CDS)

ATGGACGACGAGGGAGTGCGGTGCGTTTGCCGATCATGGCGGTCCTCTATTCTTCCTTTCCCCCAAACATCTCCTGCTCTTCGTCTTCGATTTCCTTCTCCATTCATCACTGCAAATCTCTTCGTCGATGCCTTCCTCTCCCAAAGCACACTCTATCGCCTCGCTCCTCTCGACCACGAACCAAGCTCTAACGTTTCTTCTTCGTCTTCCCCTCGCCGGAATGGCCGTCTGGTGAAGCTGGAGAAATCGGAGCTAGGTAAGATGCGATTTCTTCATCCTTTGTCCAGACGCCCAATCCGATGCAACCTGGAAGATCTTTCGGAAGAACACAATGAATTGAACTTACTGGATCTTCAAATCGACGAATTAGTTATATCCTATTCGCTCAAGTACACTGATACCACCTGTGTTCATGGAATCGCTAAAATCGTTGTATTCCCAAATTCCACCGGACCGGATGTTAAAGATTGTACCGTAATCGCAGTGTTTGAGAACGGGAAGTTAGGGTTTGCCAGATCTGGTGACGAGAAATGGACTCTAATTGACGAACAAAATTTCCACTACGACGATGTAATTGTCTACAATCAGCAATACTACACAGTCGATAGACGGGGAACAGTTTTCTGGATCGATTCATCGATGCGATTGGTACAATTTTCTCCTTCTCTGGTCGGTCTCGGCCACCATAAGCACTTAGTAGAGTGCGGCGGCGAACTTCTAGTAATCGATCGATTCCTCGACAAAGAGCGTCAGTTTCAACACCCAATCGCCGTCGTTGACGACGCACAACATCCTTTCGCGATGCCAGTTCGACGGCCGCCTTACGATTCGTCAGCAAGAGCAGTGGATTTCAAAGTTCATAAGCTGGATCAAGAATGGGGGACATGGGTGGAGCTGAAGAACTTGAGAAATCGATCGATTATATTGGGGAACGATTGTTGTTTCTCAGTGGAAGCATCGGAGTTTGAAGGATGTAAAGAGAATTGCATTTACTATACGGATCTGGACGACGGGGAATTCGTTCAAAGAAGTACGAGTCGTGTGTTTGATCTTGAAGGAAGAAGGATCGGTAAGATACTGTGTTATCCTGGACGAATCGGGATTTTCAACCCGCCGCCGATTTGGCTCTCAAAATCTCTTCGTCCATTGAAGGTACGTATACATATGAAATCTGGGTTTCTTGAGAATTATGTGATCAAACTGGGATTTCAACTTATTTCTATTGGATCTTAA

Protein sequence

MDDEGVRCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVSSSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVISYSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGRIGIFNPPPIWLSKSLRPLKVRIHMKSGFLENYVIKLGFQLISIGS
Homology
BLAST of Lsi09G007520 vs. ExPASy Swiss-Prot
Match: Q9SHX9 (Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 3.5e-28
Identity = 99/338 (29.29%), Postives = 160/338 (47.34%), Query Frame = 0

Query: 7   RCVCRSWRSSI------LPFPQTSPALRLR-FPSPFITANLFVDAFLSQSTLYRLAPLDH 66
           R +CRSWRSS+       PF +T P + L   P+  +T +     FLS+S  +R      
Sbjct: 29  RSICRSWRSSVPGAGKKNPF-RTRPLILLNPNPNKPLTDHRRRGEFLSRSAFFR------ 88

Query: 67  EPSSNVSSSSSPRRNGRLVKLEKS-ELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDL 126
                V+ SSSP + G L+K +     GK+  L PLSR P       +      ++L + 
Sbjct: 89  -----VTLSSSPSQ-GWLIKSDVDVSSGKLHLLDPLSRLP-------MEHSRKRVDLSEF 148

Query: 127 QIDELVISYSLK--YTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDE 186
            I E+  +Y +    T         ++ +  +  G    D  V+ +   GK+ +    D 
Sbjct: 149 TITEIREAYQVHDWRTRKETRPIFKRVALVKDKEG----DNQVLGIRSTGKMMY---WDI 208

Query: 187 KWTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECG 246
           K     E+ + + D+IV+  Q Y +D  G V+WI S ++ ++F P +      + LVEC 
Sbjct: 209 KTWKAKEEGYEFSDIIVHKGQTYALDSIGIVYWIRSDLKFIRFGPLVGDWTGDRRLVECC 268

Query: 247 GELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTW 306
           GE  +++R + +    +      DD  + +            A+ V FKV+K D E G  
Sbjct: 269 GEFYIVERLVGESTWKRK----ADDTGYEY------------AKTVGFKVYKFDDEQGKM 323

Query: 307 VELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYTD 335
           +E+K+L +++ ++  D CFSV A EF GC EN IY+TD
Sbjct: 329 MEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYFTD 323

BLAST of Lsi09G007520 vs. ExPASy Swiss-Prot
Match: Q1PEZ8 (F-box protein At2g26160 OS=Arabidopsis thaliana OX=3702 GN=At2g26160 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 8.6e-27
Identity = 98/346 (28.32%), Postives = 160/346 (46.24%), Query Frame = 0

Query: 6   VRCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNV 65
           VR +C+ WRS+      T  + +   PS    +N  ++  LS +T +R           V
Sbjct: 30  VRSICKPWRSA----AATPKSFQCNLPS----SNKMIETVLSPTTFFR-----------V 89

Query: 66  SSSSSPRRNGRLVKLEK-SELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELV 125
           +  SS    G L++ ++ SE  K+  L P  R       + L+     L+LL  ++ E+ 
Sbjct: 90  TGPSSCSYKGWLIRTKQVSESSKINLLSPFFR-------QLLTPSQQTLDLLKFEVSEIR 149

Query: 126 ISYSLKYTDTTCVHGIAKIVVFPNSTGPD-------VKDCTVIAVFENGKLGFARSGDEK 185
            SY +   D   + G+          GP          D  + AV ++ K+   +SG+E 
Sbjct: 150 QSYEIHIFDKYLIQGVI------GKEGPSHILSRVVFLDNLIFAVGQDDKIWCCKSGEES 209

Query: 186 ---WTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWID-SSMRLVQFSPSLV----GLGH- 245
              WT I  Q   + D+I++  Q Y +D  G ++WI  S + L+QF+PS+     G    
Sbjct: 210 SRIWTKIKNQVEDFLDIILHKGQVYALDLTGAIWWISLSPLSLLQFTPSIPMDYDGYDSC 269

Query: 246 HKHLVECGGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHK 305
           +K LVE  G+L +I +   K+                    +RR     S R V FKV+K
Sbjct: 270 NKRLVEYCGDLCIIHQLRLKKAY------------------IRR-----SQRTVGFKVYK 320

Query: 306 LDQEWGTWVELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYTD 335
           +D+    WVE+++L ++++I+  D CF+V ASE+ GC  N IY+ D
Sbjct: 330 MDEYVAKWVEVRSLGDKALIVARDSCFTVVASEYHGCLNNSIYFVD 320

BLAST of Lsi09G007520 vs. ExPASy Swiss-Prot
Match: Q3EBZ2 (F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.8e-25
Identity = 111/394 (28.17%), Postives = 186/394 (47.21%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFP-SPFITANLFVDAF----LSQSTLYRLAPLDHEP 66
           R VC SWRS+  P    SP      P  P    +LF D+     LSQ ++  + P  HEP
Sbjct: 29  RSVCSSWRSAAEP---KSPLPTHHLPILPDNGGSLFPDSAVGFRLSQRSILLIKP--HEP 88

Query: 67  SSNVSSSSSPRRNGRLVKLEK--SELGKMRFLHPLSRRPIRCNLEDLSEEH--NELNLLD 126
              + S S     G L+K+E+  +   K+  L PL      C+  +   E+    L++  
Sbjct: 89  C--IESDSF----GWLIKVEEDLNVPRKVTLLDPL------CDTRNSIPENFPRVLDMSK 148

Query: 127 LQIDELVISYSLKYTDTT--CVHG--IAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARS 186
            ++ EL   + L Y +T    V    + K VV       D K   ++ +  +GKL   RS
Sbjct: 149 FKVRELGREFKLHYFNTVGDIVESLYLEKAVVKYLDCDGDYK-FVLLTIHVSGKLAVFRS 208

Query: 187 GDEKWTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWID-SSMRLVQFSPSLVGLGHHKHL 246
            D  WT+I++    YDDV++++ +++ VD  G    +D SS++L   +  + G G  K L
Sbjct: 209 WDRAWTVINDMPSPYDDVMLFDGRFFAVDNNGRTVVVDYSSLKLTLVASPVFG-GDKKFL 268

Query: 247 VECGGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQE 306
           +E  GE+L++D +L  E     P  V +  +H        P +  + R V FKV++  + 
Sbjct: 269 IESCGEMLLVDMYLSLEAVEGDPGFVEEIFEH--------PAFYMNERTVKFKVYRFVER 328

Query: 307 WGTWVELKNLRNRSIILGNDCCFSVEASE-FEGCKENCIYYT-DLDDGEFVQRSTSR--- 366
             +WV++ +L ++ + LG+D  FS  AS+    C  + +++  ++ +GE +     R   
Sbjct: 329 EESWVDVYDLEDKMLFLGDDSTFSASASDILPLCDGSSVFFNGNVFNGEDLGAMQDRDLG 388

Query: 367 VFDLEGRRIGKILCYPGRIGIFNPPPIWLSKSLR 382
           VFD    +I  +   P    +F PPP W++   R
Sbjct: 389 VFDFRSGKIELVQKLPEYAKLFWPPPPWITSHAR 395

BLAST of Lsi09G007520 vs. ExPASy Swiss-Prot
Match: Q9LS04 (Putative F-box protein At3g25750 OS=Arabidopsis thaliana OX=3702 GN=At3g25750 PE=4 SV=2)

HSP 1 Score: 108.2 bits (269), Expect = 2.2e-22
Identity = 94/364 (25.82%), Postives = 154/364 (42.31%), Query Frame = 0

Query: 6   VRCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNV 65
           +R  C+SWRS++     +   L+ RF     T+N  + A LS +T +R           +
Sbjct: 30  IRSTCKSWRSAV---AMSKERLQFRFERYLPTSNKKIKAHLSPTTFFR-----------I 89

Query: 66  SSSSSPRRNGRLVKLEKSE--LGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDEL 125
           +  SS    G LV+  ++     K+  L PLS        E ++  H  L+LL + + E+
Sbjct: 90  TLPSSCPNKGWLVRTRQASKMYRKITLLCPLSG-------ERITRSHQTLDLLKVGVSEI 149

Query: 126 VISYSLKYTDTTCVHGI-AKIVVFPNSTGPDVKDCTVIAVFENGKLGF--ARSGDEKWTL 185
             SY ++  D      I     +F N      K  +V   + + K+     R G   WT 
Sbjct: 150 RQSYEIQIFDGLKDEKIPLDSEIFSNYIKNSDKIPSV--NYHDNKIWCCKTREGSRSWTK 209

Query: 186 IDEQNFHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKH-------LV 245
           I  Q   + D+I++  + Y VD +G ++WI  S   +    S   L ++K+       LV
Sbjct: 210 IKNQVEDFSDIILHMGRIYAVDLKGAIWWISLSQLTIVQQTSSTPLDYYKYDSCQDTRLV 269

Query: 246 ECGGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEW 305
           E  G+L ++          Q                          R V FKV+K+D++ 
Sbjct: 270 EYCGDLCIVHELSITRNHIQ--------------------------RTVGFKVYKMDEDL 329

Query: 306 GTWVELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYT-------------DLDDGEF 345
             WVE+  L + ++I+  + CF+V ASE+ GC +N IY++              LDDG  
Sbjct: 330 AKWVEVSCLGDNTLIVACNSCFTVVASEYHGCLKNSIYFSYYDVKKAENIKVFKLDDGSI 344

BLAST of Lsi09G007520 vs. ExPASy Swiss-Prot
Match: Q9LVG8 (Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 3.8e-22
Identity = 103/350 (29.43%), Postives = 169/350 (48.29%), Query Frame = 0

Query: 6   VRCVCRSWRSSILPFPQTSPALRLRFPS--PFITANLFVDAF--LSQSTLYRL-APLDHE 65
           +R VC +WR S LP    +  L  +FP   PF +++     F  L QS +Y+L APL   
Sbjct: 39  LRSVCATWRLS-LPLSNKNNRLS-KFPKYLPFWSSSSSSSGFFTLKQSNVYKLEAPL--- 98

Query: 66  PSSNVSSSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQI 125
                    +PR    LVKL+++  G MR L   S   I C L +     ++++L +  +
Sbjct: 99  ---------NPRT--CLVKLQETTPGIMRVLDLFSNDRI-CFLPE--NFPSKIDLQEFHV 158

Query: 126 DELVISYSLKYTD-----TTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSG- 185
             +  +Y ++Y +       C   +    V   S+G   +D  +IA+   GKLGF +SG 
Sbjct: 159 RLVRRTYRMEYANNGGGAVPCFWSLHSDKVVILSSG---EDSAIIAIHSGGKLGFLKSGN 218

Query: 186 DEKWTLIDEQ-NFHYDDVIVY-NQQYYTVDRRGTVFWIDSSMRLVQFSPSLV-GLGHHKH 245
           DEKW ++D   N  Y+D+++Y + +   VD +G     D   ++   +  LV G GH KH
Sbjct: 219 DEKWKILDNSWNVIYEDIMLYKDNRCIVVDDKGKTVIYDVDFKVSDLAEGLVGGGGHKKH 278

Query: 246 LVEC-GGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLD 305
           LVEC GGE+ ++D++                      +       D S   V+F+V+ L 
Sbjct: 279 LVECSGGEVFLVDKY----------------------VKTVWCKSDISKSVVEFRVYNLK 338

Query: 306 QEWGTWVELKNLRNRSIILGNDCCFSVE--ASEFEGCKENCIYYTDLDDG 339
           +E   W E+++L + ++ +G+DC FSV+  A +  G     I+Y+D  +G
Sbjct: 339 REEKRWEEVRDLGDVALFIGDDCSFSVQNPAGDLAG---GFIFYSDYRNG 341

BLAST of Lsi09G007520 vs. ExPASy TrEMBL
Match: A0A5A7VPR3 (Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold174G00870 PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 6.6e-179
Identity = 310/383 (80.94%), Postives = 341/383 (89.03%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ PF QTSP L LRFPSP+I+ANL VDAFLSQ+ +YRLAPL+HEPS NVS
Sbjct: 33  RSVCRSWRSSLPPFTQTSPPLPLRFPSPYISANLSVDAFLSQTIIYRLAPLNHEPSPNVS 92

Query: 67  SSSS-PRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVI 126
           SSSS P RNGRLVK+EKSELGKMRFLHPLS++ IRCNLEDLS+ HNELNLLDL++DEL +
Sbjct: 93  SSSSPPPRNGRLVKVEKSELGKMRFLHPLSKQIIRCNLEDLSQGHNELNLLDLRMDELAV 152

Query: 127 SYSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQN 186
           SYSLKY+DTTCV GI+KIVVFPNS+G DVKDCT+IAVFE GKLGFARSGDEKWTLIDEQN
Sbjct: 153 SYSLKYSDTTCVPGISKIVVFPNSSGSDVKDCTIIAVFECGKLGFARSGDEKWTLIDEQN 212

Query: 187 FHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRF 246
           FHYDDVIVYNQQYYTVDR GTVFWIDSSMRLVQFSP L+GLGHHKHLVECGGELLVIDRF
Sbjct: 213 FHYDDVIVYNQQYYTVDRWGTVFWIDSSMRLVQFSPPLIGLGHHKHLVECGGELLVIDRF 272

Query: 247 LDKERQFQHPIAVVDDA---QHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNL 306
           LDKER FQ  IA VDDA   QH F +PV+RPPY S  +AVDFK +KLDQEWGTWVELKNL
Sbjct: 273 LDKERHFQPAIAAVDDAEQQQHHFMIPVQRPPYSSLPKAVDFKAYKLDQEWGTWVELKNL 332

Query: 307 RNRSIILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCY 366
            NRSIILGNDCCFS+EASEFEGCK+NC+YYTD+ +  F +RS SRVFD+EG+RIG IL Y
Sbjct: 333 GNRSIILGNDCCFSIEASEFEGCKKNCVYYTDVTEDAFSKRSRSRVFDVEGKRIGNILGY 392

Query: 367 PGR-IGIFNPPPIWLSKSLRPLK 385
           PGR IGIFN PPIWLSKS+ PLK
Sbjct: 393 PGRMIGIFNTPPIWLSKSIPPLK 415

BLAST of Lsi09G007520 vs. ExPASy TrEMBL
Match: A0A1S3CRG5 (putative F-box protein At1g65770 OS=Cucumis melo OX=3656 GN=LOC103503900 PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 6.6e-179
Identity = 310/383 (80.94%), Postives = 341/383 (89.03%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ PF QTSP L LRFPSP+I+ANL VDAFLSQ+ +YRLAPL+HEPS NVS
Sbjct: 33  RSVCRSWRSSLPPFTQTSPPLPLRFPSPYISANLSVDAFLSQTIIYRLAPLNHEPSPNVS 92

Query: 67  SSSS-PRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVI 126
           SSSS P RNGRLVK+EKSELGKMRFLHPLS++ IRCNLEDLS+ HNELNLLDL++DEL +
Sbjct: 93  SSSSPPPRNGRLVKVEKSELGKMRFLHPLSKQIIRCNLEDLSQGHNELNLLDLRMDELAV 152

Query: 127 SYSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQN 186
           SYSLKY+DTTCV GI+KIVVFPNS+G DVKDCT+IAVFE GKLGFARSGDEKWTLIDEQN
Sbjct: 153 SYSLKYSDTTCVPGISKIVVFPNSSGSDVKDCTIIAVFECGKLGFARSGDEKWTLIDEQN 212

Query: 187 FHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRF 246
           FHYDDVIVYNQQYYTVDR GTVFWIDSSMRLVQFSP L+GLGHHKHLVECGGELLVIDRF
Sbjct: 213 FHYDDVIVYNQQYYTVDRWGTVFWIDSSMRLVQFSPPLIGLGHHKHLVECGGELLVIDRF 272

Query: 247 LDKERQFQHPIAVVDDA---QHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNL 306
           LDKER FQ  IA VDDA   QH F +PV+RPPY S  +AVDFK +KLDQEWGTWVELKNL
Sbjct: 273 LDKERHFQPAIAAVDDAEQQQHHFMIPVQRPPYSSLPKAVDFKAYKLDQEWGTWVELKNL 332

Query: 307 RNRSIILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCY 366
            NRSIILGNDCCFS+EASEFEGCK+NC+YYTD+ +  F +RS SRVFD+EG+RIG IL Y
Sbjct: 333 GNRSIILGNDCCFSIEASEFEGCKKNCVYYTDVTEDAFSKRSRSRVFDVEGKRIGNILGY 392

Query: 367 PGR-IGIFNPPPIWLSKSLRPLK 385
           PGR IGIFN PPIWLSKS+ PLK
Sbjct: 393 PGRMIGIFNTPPIWLSKSIPPLK 415

BLAST of Lsi09G007520 vs. ExPASy TrEMBL
Match: A0A6J1GFH5 (uncharacterized protein LOC111453467 OS=Cucurbita moschata OX=3662 GN=LOC111453467 PE=4 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 3.4e-175
Identity = 306/378 (80.95%), Postives = 327/378 (86.51%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ P P+ SP +RLRFPSP  TA+LFVDAFLSQST YRLAPL HE S N  
Sbjct: 469 RSVCRSWRSSVPPVPKISPPIRLRFPSPINTASLFVDAFLSQSTFYRLAPLIHEQSPN-- 528

Query: 67  SSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVIS 126
            SSS  +NGRLVKLEKSELGKMRFLHPLS+R +RCN     EEH ELNLLDL+IDEL IS
Sbjct: 529 -SSSSNQNGRLVKLEKSELGKMRFLHPLSKRLMRCN----PEEHKELNLLDLRIDELAIS 588

Query: 127 YSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNF 186
           YSLKYTDT CV GIAK++VFP STGPDV+DCTVIAVFE+GKLGFARSGDEKWTLIDEQNF
Sbjct: 589 YSLKYTDTACVPGIAKVMVFPKSTGPDVEDCTVIAVFEDGKLGFARSGDEKWTLIDEQNF 648

Query: 187 HYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFL 246
           HYDDVIVYN+QYY VDR GTVFWIDSSMRLVQFSP L+GLG  KHLVECGGELLVIDRFL
Sbjct: 649 HYDDVIVYNRQYYAVDRWGTVFWIDSSMRLVQFSPPLIGLGQQKHLVECGGELLVIDRFL 708

Query: 247 DKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNRS 306
           DKER  QHP  ++DDA HP A+P RRP  DSS RAVDFK HKLDQEWGTWVELKNL NRS
Sbjct: 709 DKERSVQHPTDIMDDA-HPMAIPFRRPDNDSSPRAVDFKAHKLDQEWGTWVELKNLGNRS 768

Query: 307 IILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGRI 366
           IILGNDCC SVEASEFEGCKENCIYYTD++D EF +RS SRVFDLEG RIG ILCYPGRI
Sbjct: 769 IILGNDCCLSVEASEFEGCKENCIYYTDVNDNEFSKRSFSRVFDLEGGRIGNILCYPGRI 828

Query: 367 GIFNPPPIWLSKSLRPLK 385
           GIF+PPPIWLSKSL PLK
Sbjct: 829 GIFSPPPIWLSKSLPPLK 838

BLAST of Lsi09G007520 vs. ExPASy TrEMBL
Match: A0A6J1IRW4 (putative F-box protein At1g65770 OS=Cucurbita maxima OX=3661 GN=LOC111478154 PE=4 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.4e-173
Identity = 305/378 (80.69%), Postives = 324/378 (85.71%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ P P+ SP +RLRFPSP  TA+LFVDAFLSQST YRLAPL HE S N  
Sbjct: 32  RSVCRSWRSSVPPVPKVSPPIRLRFPSPINTASLFVDAFLSQSTFYRLAPLIHEQSPN-- 91

Query: 67  SSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVIS 126
            SSS  +NGRLVKLEKSELGKMRFLHPLS+R IRCN     EEH ELNLLDL+IDEL IS
Sbjct: 92  -SSSYNQNGRLVKLEKSELGKMRFLHPLSKRRIRCN----PEEHKELNLLDLRIDELAIS 151

Query: 127 YSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNF 186
           YSLKYTDT CV GIAK+VV P STGPDV+DCTVIAVFE+GKLGFAR GDEKWTLIDEQNF
Sbjct: 152 YSLKYTDTACVPGIAKVVVLPTSTGPDVEDCTVIAVFEDGKLGFARCGDEKWTLIDEQNF 211

Query: 187 HYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFL 246
           HYDDVIVYN+QYY VDR GTVFWIDSSMRLVQFSP L+GLG  KHLVECGGELLVIDRFL
Sbjct: 212 HYDDVIVYNRQYYAVDRWGTVFWIDSSMRLVQFSPPLIGLGQQKHLVECGGELLVIDRFL 271

Query: 247 DKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNRS 306
           DKER  QHP  ++DDA HP A+P RRP  DSS RAVDFK HKLDQEWGTWVELKNL NRS
Sbjct: 272 DKERSVQHPTDIMDDA-HPIAIPFRRPDNDSSPRAVDFKAHKLDQEWGTWVELKNLGNRS 331

Query: 307 IILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGRI 366
           IILGNDCC SVEASEFEGCKENCIYY D++D EF +RS SRVFDLEGRRIG IL YPGRI
Sbjct: 332 IILGNDCCLSVEASEFEGCKENCIYYADVNDNEFSKRSCSRVFDLEGRRIGNILGYPGRI 391

Query: 367 GIFNPPPIWLSKSLRPLK 385
           GIF+PPPIWLSKSL PLK
Sbjct: 392 GIFSPPPIWLSKSLPPLK 401

BLAST of Lsi09G007520 vs. ExPASy TrEMBL
Match: A0A0A0KLM6 (DUF295 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G118170 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 8.3e-166
Identity = 291/385 (75.58%), Postives = 328/385 (85.19%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ PF QTSP L L FPSP+I+  L + AFLSQ+TLYRL PL+H+P+ N+S
Sbjct: 58  RSVCRSWRSSLPPFSQTSPPLPLPFPSPYISPTLPLHAFLSQTTLYRLGPLNHQPTPNLS 117

Query: 67  SSSSPR-RNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVI 126
           SSSSP   NGRLVK+EKSELGKMRFLHPLS++ I  NLED SE HNE NLL L+I EL +
Sbjct: 118 SSSSPTPPNGRLVKVEKSELGKMRFLHPLSKKIIPRNLEDPSEGHNEFNLLHLRIHELAV 177

Query: 127 SYSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQN 186
           SYSLKYTDTTCV GI+KIV+FPNS+G DVKDCT+IAVFE GKLGFARSGDEKWTLIDEQN
Sbjct: 178 SYSLKYTDTTCVPGISKIVLFPNSSGSDVKDCTIIAVFEGGKLGFARSGDEKWTLIDEQN 237

Query: 187 FHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRF 246
           FHYDDVIVYNQQYYTVDR GTVFWID SMRLVQFSP L+GLGH KHLVECGGELLVIDRF
Sbjct: 238 FHYDDVIVYNQQYYTVDRWGTVFWIDLSMRLVQFSPPLIGLGHQKHLVECGGELLVIDRF 297

Query: 247 LDKERQFQHPIAVVDDA-----QHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELK 306
           L+KER FQ  IA V+DA     Q PF +PVR  PY+S  +AVDFK +KLDQEWGTWVELK
Sbjct: 298 LNKERHFQPVIAAVEDAEVQQQQQPFIIPVRHTPYNSLPKAVDFKAYKLDQEWGTWVELK 357

Query: 307 NLRNRSIILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKIL 366
           NLRN+SIILGNDCCFS+EASEFEGCK+NC+YYTD+ + EF +RSTSRVFD+EG+R G IL
Sbjct: 358 NLRNQSIILGNDCCFSIEASEFEGCKKNCVYYTDVREDEFCKRSTSRVFDVEGKRFGNIL 417

Query: 367 CYPGR-IGIFNPPPIWLSKSLRPLK 385
            +PGR I IFN PPIWLS+S+ PLK
Sbjct: 418 GHPGRMIEIFNSPPIWLSQSIPPLK 442

BLAST of Lsi09G007520 vs. NCBI nr
Match: XP_038876198.1 (F-box protein At2g26160-like [Benincasa hispida])

HSP 1 Score: 681.8 bits (1758), Expect = 3.7e-192
Identity = 330/379 (87.07%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSSI PFPQTSPALRLRFPSPFITA+L VDAFLSQ+TLYRLAPLDHEPS +VS
Sbjct: 33  RSVCRSWRSSISPFPQTSPALRLRFPSPFITASLLVDAFLSQTTLYRLAPLDHEPSPSVS 92

Query: 67  SSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVIS 126
           SSSSPRRNGRLVKLEKSELGKMRFLHPLS+R IR N+EDLSEEHNELNLLDL+IDEL IS
Sbjct: 93  SSSSPRRNGRLVKLEKSELGKMRFLHPLSKRQIRYNMEDLSEEHNELNLLDLRIDELAIS 152

Query: 127 YSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNF 186
           YSLKYTDT  V GI KIVVFPNST PDVKDCTVIAVFE+G+LGFARS DEKWTLIDEQNF
Sbjct: 153 YSLKYTDTPFVPGIPKIVVFPNSTAPDVKDCTVIAVFEDGRLGFARSDDEKWTLIDEQNF 212

Query: 187 HYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFL 246
           HYDDVI+YN QYYTVDR GTVFWIDSSMRLVQFSP L+GLGH KHLVECGGELLVIDRFL
Sbjct: 213 HYDDVIIYNHQYYTVDRWGTVFWIDSSMRLVQFSPPLIGLGHQKHLVECGGELLVIDRFL 272

Query: 247 DKERQFQHPIAVVDDAQ-HPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNR 306
           DKERQFQHP+  VDDAQ HPFA+PVRRP YDS  +AVDFK +KLDQEWGTWVELKNLRNR
Sbjct: 273 DKERQFQHPVPAVDDAQPHPFAIPVRRPSYDSLPKAVDFKAYKLDQEWGTWVELKNLRNR 332

Query: 307 SIILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGR 366
           SIILG DCCFS+EASEFEGCKENCIYYTD++D EF +RS SRVFDLEGRRIG IL YPGR
Sbjct: 333 SIILGTDCCFSIEASEFEGCKENCIYYTDVNDDEFSKRSMSRVFDLEGRRIGNILGYPGR 392

Query: 367 IGIFNPPPIWLSKSLRPLK 385
           IGIFNPPPIWLSKSL PLK
Sbjct: 393 IGIFNPPPIWLSKSLPPLK 411

BLAST of Lsi09G007520 vs. NCBI nr
Match: XP_008466513.1 (PREDICTED: putative F-box protein At1g65770 [Cucumis melo] >KAA0067551.1 putative F-box protein [Cucumis melo var. makuwa] >TYJ97203.1 putative F-box protein [Cucumis melo var. makuwa])

HSP 1 Score: 636.7 bits (1641), Expect = 1.4e-178
Identity = 310/383 (80.94%), Postives = 341/383 (89.03%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ PF QTSP L LRFPSP+I+ANL VDAFLSQ+ +YRLAPL+HEPS NVS
Sbjct: 33  RSVCRSWRSSLPPFTQTSPPLPLRFPSPYISANLSVDAFLSQTIIYRLAPLNHEPSPNVS 92

Query: 67  SSSS-PRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVI 126
           SSSS P RNGRLVK+EKSELGKMRFLHPLS++ IRCNLEDLS+ HNELNLLDL++DEL +
Sbjct: 93  SSSSPPPRNGRLVKVEKSELGKMRFLHPLSKQIIRCNLEDLSQGHNELNLLDLRMDELAV 152

Query: 127 SYSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQN 186
           SYSLKY+DTTCV GI+KIVVFPNS+G DVKDCT+IAVFE GKLGFARSGDEKWTLIDEQN
Sbjct: 153 SYSLKYSDTTCVPGISKIVVFPNSSGSDVKDCTIIAVFECGKLGFARSGDEKWTLIDEQN 212

Query: 187 FHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRF 246
           FHYDDVIVYNQQYYTVDR GTVFWIDSSMRLVQFSP L+GLGHHKHLVECGGELLVIDRF
Sbjct: 213 FHYDDVIVYNQQYYTVDRWGTVFWIDSSMRLVQFSPPLIGLGHHKHLVECGGELLVIDRF 272

Query: 247 LDKERQFQHPIAVVDDA---QHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNL 306
           LDKER FQ  IA VDDA   QH F +PV+RPPY S  +AVDFK +KLDQEWGTWVELKNL
Sbjct: 273 LDKERHFQPAIAAVDDAEQQQHHFMIPVQRPPYSSLPKAVDFKAYKLDQEWGTWVELKNL 332

Query: 307 RNRSIILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCY 366
            NRSIILGNDCCFS+EASEFEGCK+NC+YYTD+ +  F +RS SRVFD+EG+RIG IL Y
Sbjct: 333 GNRSIILGNDCCFSIEASEFEGCKKNCVYYTDVTEDAFSKRSRSRVFDVEGKRIGNILGY 392

Query: 367 PGR-IGIFNPPPIWLSKSLRPLK 385
           PGR IGIFN PPIWLSKS+ PLK
Sbjct: 393 PGRMIGIFNTPPIWLSKSIPPLK 415

BLAST of Lsi09G007520 vs. NCBI nr
Match: XP_022950355.1 (uncharacterized protein LOC111453467 [Cucurbita moschata])

HSP 1 Score: 624.4 bits (1609), Expect = 7.0e-175
Identity = 306/378 (80.95%), Postives = 327/378 (86.51%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ P P+ SP +RLRFPSP  TA+LFVDAFLSQST YRLAPL HE S N  
Sbjct: 469 RSVCRSWRSSVPPVPKISPPIRLRFPSPINTASLFVDAFLSQSTFYRLAPLIHEQSPN-- 528

Query: 67  SSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVIS 126
            SSS  +NGRLVKLEKSELGKMRFLHPLS+R +RCN     EEH ELNLLDL+IDEL IS
Sbjct: 529 -SSSSNQNGRLVKLEKSELGKMRFLHPLSKRLMRCN----PEEHKELNLLDLRIDELAIS 588

Query: 127 YSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNF 186
           YSLKYTDT CV GIAK++VFP STGPDV+DCTVIAVFE+GKLGFARSGDEKWTLIDEQNF
Sbjct: 589 YSLKYTDTACVPGIAKVMVFPKSTGPDVEDCTVIAVFEDGKLGFARSGDEKWTLIDEQNF 648

Query: 187 HYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFL 246
           HYDDVIVYN+QYY VDR GTVFWIDSSMRLVQFSP L+GLG  KHLVECGGELLVIDRFL
Sbjct: 649 HYDDVIVYNRQYYAVDRWGTVFWIDSSMRLVQFSPPLIGLGQQKHLVECGGELLVIDRFL 708

Query: 247 DKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNRS 306
           DKER  QHP  ++DDA HP A+P RRP  DSS RAVDFK HKLDQEWGTWVELKNL NRS
Sbjct: 709 DKERSVQHPTDIMDDA-HPMAIPFRRPDNDSSPRAVDFKAHKLDQEWGTWVELKNLGNRS 768

Query: 307 IILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGRI 366
           IILGNDCC SVEASEFEGCKENCIYYTD++D EF +RS SRVFDLEG RIG ILCYPGRI
Sbjct: 769 IILGNDCCLSVEASEFEGCKENCIYYTDVNDNEFSKRSFSRVFDLEGGRIGNILCYPGRI 828

Query: 367 GIFNPPPIWLSKSLRPLK 385
           GIF+PPPIWLSKSL PLK
Sbjct: 829 GIFSPPPIWLSKSLPPLK 838

BLAST of Lsi09G007520 vs. NCBI nr
Match: KAG6603875.1 (putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623.2 bits (1606), Expect = 1.6e-174
Identity = 306/378 (80.95%), Postives = 327/378 (86.51%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ P P+ SP +RLRFPSP  TA+LFVDAFLSQST YRLAPL +E S N  
Sbjct: 32  RSVCRSWRSSVPPVPKISPPIRLRFPSPINTASLFVDAFLSQSTFYRLAPLIYEQSPN-- 91

Query: 67  SSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVIS 126
            SSS  +NGRLVKLEKSELGKMRFLHPLS+R +RCN     EEH ELNLLDL+IDEL IS
Sbjct: 92  -SSSSNQNGRLVKLEKSELGKMRFLHPLSKRLMRCN----PEEHKELNLLDLRIDELAIS 151

Query: 127 YSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNF 186
           YSLKYTDT CV GIAK+VVFP STGPDV+DCTVIAVFE+GKLGFARSGDEKWTLIDEQNF
Sbjct: 152 YSLKYTDTACVPGIAKVVVFPKSTGPDVEDCTVIAVFEDGKLGFARSGDEKWTLIDEQNF 211

Query: 187 HYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFL 246
           HYDDVIVYN+QYY VDR GTVFWIDSSMRLVQFSP L+GLG  KHLVECGGELLVIDRFL
Sbjct: 212 HYDDVIVYNRQYYAVDRWGTVFWIDSSMRLVQFSPPLIGLGQQKHLVECGGELLVIDRFL 271

Query: 247 DKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNRS 306
           DKER  QHP  ++DDA HP A+P RRP  DSS RAVDFK HKLDQEWGTWVELKNL NRS
Sbjct: 272 DKERSVQHPTDIMDDA-HPMAIPFRRPDNDSSPRAVDFKAHKLDQEWGTWVELKNLGNRS 331

Query: 307 IILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGRI 366
           IILGNDCC SVEASEFEGCKENCIYYTD++D EF +RS SRVFDLEG RIG ILCYPGRI
Sbjct: 332 IILGNDCCLSVEASEFEGCKENCIYYTDVNDNEFSKRSFSRVFDLEGGRIGNILCYPGRI 391

Query: 367 GIFNPPPIWLSKSLRPLK 385
           GIF+PPPIWLSKSL PLK
Sbjct: 392 GIFSPPPIWLSKSLPPLK 401

BLAST of Lsi09G007520 vs. NCBI nr
Match: XP_023543888.1 (putative F-box protein At1g65770 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620.9 bits (1600), Expect = 7.7e-174
Identity = 305/378 (80.69%), Postives = 326/378 (86.24%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNVS 66
           R VCRSWRSS+ P P+ SP +RLRFPSP  TA+LFVDAFLSQST YRLAPL HE S N S
Sbjct: 32  RSVCRSWRSSVPPVPKISPPIRLRFPSPINTASLFVDAFLSQSTFYRLAPLIHEQSPNSS 91

Query: 67  SSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELVIS 126
           SSS   +NGRLVK+EKSELGKMRFLHPLS+R IRCN     EEH ELNLLDL+IDEL IS
Sbjct: 92  SSS---QNGRLVKMEKSELGKMRFLHPLSKRLIRCN----PEEHKELNLLDLRIDELAIS 151

Query: 127 YSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDEQNF 186
           YSLKYTDT CV GIAK+VVFP S GPDV+DCTVIAVFE+GKLGFARSGDEKWTLIDEQ+F
Sbjct: 152 YSLKYTDTACVPGIAKVVVFPTSIGPDVEDCTVIAVFEDGKLGFARSGDEKWTLIDEQSF 211

Query: 187 HYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECGGELLVIDRFL 246
           HYDDVIVYN+QYY VDR GTVFWIDSSMRLVQFSP L+GLG  KHLVECGGELLVIDRFL
Sbjct: 212 HYDDVIVYNRQYYAVDRWGTVFWIDSSMRLVQFSPPLIGLGQQKHLVECGGELLVIDRFL 271

Query: 247 DKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTWVELKNLRNRS 306
           DKER  QHP  ++DDA HP A+P RRP  DSS RAVDFK HKLDQEWGTWVELKNL NRS
Sbjct: 272 DKERSVQHPTDIMDDA-HPMAIPFRRPDNDSSPRAVDFKAHKLDQEWGTWVELKNLGNRS 331

Query: 307 IILGNDCCFSVEASEFEGCKENCIYYTDLDDGEFVQRSTSRVFDLEGRRIGKILCYPGRI 366
           IILGNDCC S EASEFEGCKENCIYYTD++D EF +RS SRVFDLEG RIG ILCYPGRI
Sbjct: 332 IILGNDCCLSFEASEFEGCKENCIYYTDVNDNEFSKRSFSRVFDLEGGRIGNILCYPGRI 391

Query: 367 GIFNPPPIWLSKSLRPLK 385
           GIF+PPPIWLSKSL PLK
Sbjct: 392 GIFSPPPIWLSKSLPPLK 401

BLAST of Lsi09G007520 vs. TAIR 10
Match: AT1G65770.1 (ascorbic acid mannose pathway regulator 1 )

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-29
Identity = 99/338 (29.29%), Postives = 160/338 (47.34%), Query Frame = 0

Query: 7   RCVCRSWRSSI------LPFPQTSPALRLR-FPSPFITANLFVDAFLSQSTLYRLAPLDH 66
           R +CRSWRSS+       PF +T P + L   P+  +T +     FLS+S  +R      
Sbjct: 29  RSICRSWRSSVPGAGKKNPF-RTRPLILLNPNPNKPLTDHRRRGEFLSRSAFFR------ 88

Query: 67  EPSSNVSSSSSPRRNGRLVKLEKS-ELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDL 126
                V+ SSSP + G L+K +     GK+  L PLSR P       +      ++L + 
Sbjct: 89  -----VTLSSSPSQ-GWLIKSDVDVSSGKLHLLDPLSRLP-------MEHSRKRVDLSEF 148

Query: 127 QIDELVISYSLK--YTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDE 186
            I E+  +Y +    T         ++ +  +  G    D  V+ +   GK+ +    D 
Sbjct: 149 TITEIREAYQVHDWRTRKETRPIFKRVALVKDKEG----DNQVLGIRSTGKMMY---WDI 208

Query: 187 KWTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKHLVECG 246
           K     E+ + + D+IV+  Q Y +D  G V+WI S ++ ++F P +      + LVEC 
Sbjct: 209 KTWKAKEEGYEFSDIIVHKGQTYALDSIGIVYWIRSDLKFIRFGPLVGDWTGDRRLVECC 268

Query: 247 GELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTW 306
           GE  +++R + +    +      DD  + +            A+ V FKV+K D E G  
Sbjct: 269 GEFYIVERLVGESTWKRK----ADDTGYEY------------AKTVGFKVYKFDDEQGKM 323

Query: 307 VELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYTD 335
           +E+K+L +++ ++  D CFSV A EF GC EN IY+TD
Sbjct: 329 MEVKSLGDKAFVIATDTCFSVLAHEFYGCLENAIYFTD 323

BLAST of Lsi09G007520 vs. TAIR 10
Match: AT2G26160.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 122.9 bits (307), Expect = 6.1e-28
Identity = 98/346 (28.32%), Postives = 160/346 (46.24%), Query Frame = 0

Query: 6   VRCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNV 65
           VR +C+ WRS+      T  + +   PS    +N  ++  LS +T +R           V
Sbjct: 30  VRSICKPWRSA----AATPKSFQCNLPS----SNKMIETVLSPTTFFR-----------V 89

Query: 66  SSSSSPRRNGRLVKLEK-SELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDELV 125
           +  SS    G L++ ++ SE  K+  L P  R       + L+     L+LL  ++ E+ 
Sbjct: 90  TGPSSCSYKGWLIRTKQVSESSKINLLSPFFR-------QLLTPSQQTLDLLKFEVSEIR 149

Query: 126 ISYSLKYTDTTCVHGIAKIVVFPNSTGPD-------VKDCTVIAVFENGKLGFARSGDEK 185
            SY +   D   + G+          GP          D  + AV ++ K+   +SG+E 
Sbjct: 150 QSYEIHIFDKYLIQGVI------GKEGPSHILSRVVFLDNLIFAVGQDDKIWCCKSGEES 209

Query: 186 ---WTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWID-SSMRLVQFSPSLV----GLGH- 245
              WT I  Q   + D+I++  Q Y +D  G ++WI  S + L+QF+PS+     G    
Sbjct: 210 SRIWTKIKNQVEDFLDIILHKGQVYALDLTGAIWWISLSPLSLLQFTPSIPMDYDGYDSC 269

Query: 246 HKHLVECGGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHK 305
           +K LVE  G+L +I +   K+                    +RR     S R V FKV+K
Sbjct: 270 NKRLVEYCGDLCIIHQLRLKKAY------------------IRR-----SQRTVGFKVYK 320

Query: 306 LDQEWGTWVELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYTD 335
           +D+    WVE+++L ++++I+  D CF+V ASE+ GC  N IY+ D
Sbjct: 330 MDEYVAKWVEVRSLGDKALIVARDSCFTVVASEYHGCLNNSIYFVD 320

BLAST of Lsi09G007520 vs. TAIR 10
Match: AT2G17030.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 117.9 bits (294), Expect = 2.0e-26
Identity = 111/394 (28.17%), Postives = 186/394 (47.21%), Query Frame = 0

Query: 7   RCVCRSWRSSILPFPQTSPALRLRFP-SPFITANLFVDAF----LSQSTLYRLAPLDHEP 66
           R VC SWRS+  P    SP      P  P    +LF D+     LSQ ++  + P  HEP
Sbjct: 29  RSVCSSWRSAAEP---KSPLPTHHLPILPDNGGSLFPDSAVGFRLSQRSILLIKP--HEP 88

Query: 67  SSNVSSSSSPRRNGRLVKLEK--SELGKMRFLHPLSRRPIRCNLEDLSEEH--NELNLLD 126
              + S S     G L+K+E+  +   K+  L PL      C+  +   E+    L++  
Sbjct: 89  C--IESDSF----GWLIKVEEDLNVPRKVTLLDPL------CDTRNSIPENFPRVLDMSK 148

Query: 127 LQIDELVISYSLKYTDTT--CVHG--IAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARS 186
            ++ EL   + L Y +T    V    + K VV       D K   ++ +  +GKL   RS
Sbjct: 149 FKVRELGREFKLHYFNTVGDIVESLYLEKAVVKYLDCDGDYK-FVLLTIHVSGKLAVFRS 208

Query: 187 GDEKWTLIDEQNFHYDDVIVYNQQYYTVDRRGTVFWID-SSMRLVQFSPSLVGLGHHKHL 246
            D  WT+I++    YDDV++++ +++ VD  G    +D SS++L   +  + G G  K L
Sbjct: 209 WDRAWTVINDMPSPYDDVMLFDGRFFAVDNNGRTVVVDYSSLKLTLVASPVFG-GDKKFL 268

Query: 247 VECGGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQE 306
           +E  GE+L++D +L  E     P  V +  +H        P +  + R V FKV++  + 
Sbjct: 269 IESCGEMLLVDMYLSLEAVEGDPGFVEEIFEH--------PAFYMNERTVKFKVYRFVER 328

Query: 307 WGTWVELKNLRNRSIILGNDCCFSVEASE-FEGCKENCIYYT-DLDDGEFVQRSTSR--- 366
             +WV++ +L ++ + LG+D  FS  AS+    C  + +++  ++ +GE +     R   
Sbjct: 329 EESWVDVYDLEDKMLFLGDDSTFSASASDILPLCDGSSVFFNGNVFNGEDLGAMQDRDLG 388

Query: 367 VFDLEGRRIGKILCYPGRIGIFNPPPIWLSKSLR 382
           VFD    +I  +   P    +F PPP W++   R
Sbjct: 389 VFDFRSGKIELVQKLPEYAKLFWPPPPWITSHAR 395

BLAST of Lsi09G007520 vs. TAIR 10
Match: AT3G25750.1 (F-box family protein with a domain of unknown function (DUF295) )

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-23
Identity = 89/361 (24.65%), Postives = 147/361 (40.72%), Query Frame = 0

Query: 6   VRCVCRSWRSSILPFPQTSPALRLRFPSPFITANLFVDAFLSQSTLYRLAPLDHEPSSNV 65
           +R  C+SWRS++     +   L+ RF     T+N  + A LS +T +R           +
Sbjct: 30  IRSTCKSWRSAV---AMSKERLQFRFERYLPTSNKKIKAHLSPTTFFR-----------I 89

Query: 66  SSSSSPRRNGRLVKLEKSE--LGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQIDEL 125
           +  SS    G LV+  ++     K+  L PLS        E ++  H  L+LL + + E+
Sbjct: 90  TLPSSCPNKGWLVRTRQASKMYRKITLLCPLSG-------ERITRSHQTLDLLKVGVSEI 149

Query: 126 VISYSLKYTDTTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSGDEKWTLIDE 185
             SY ++  D      I               D  + + +          G   WT I  
Sbjct: 150 RQSYEIQIFDGLKDEKIP-------------LDSEIFSNYIKNSDKIPSEGSRSWTKIKN 209

Query: 186 QNFHYDDVIVYNQQYYTVDRRGTVFWIDSSMRLVQFSPSLVGLGHHKH-------LVECG 245
           Q   + D+I++  + Y VD +G ++WI  S   +    S   L ++K+       LVE  
Sbjct: 210 QVEDFSDIILHMGRIYAVDLKGAIWWISLSQLTIVQQTSSTPLDYYKYDSCQDTRLVEYC 269

Query: 246 GELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLDQEWGTW 305
           G+L ++          Q                          R V FKV+K+D++   W
Sbjct: 270 GDLCIVHELSITRNHIQ--------------------------RTVGFKVYKMDEDLAKW 329

Query: 306 VELKNLRNRSIILGNDCCFSVEASEFEGCKENCIYYT-------------DLDDGEFVQR 345
           VE+  L + ++I+  + CF+V ASE+ GC +N IY++              LDDG   QR
Sbjct: 330 VEVSCLGDNTLIVACNSCFTVVASEYHGCLKNSIYFSYYDVKKAENIKVFKLDDGSITQR 330

BLAST of Lsi09G007520 vs. TAIR 10
Match: AT5G60060.1 (Protein of unknown function (DUF295) )

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-23
Identity = 103/350 (29.43%), Postives = 169/350 (48.29%), Query Frame = 0

Query: 6   VRCVCRSWRSSILPFPQTSPALRLRFPS--PFITANLFVDAF--LSQSTLYRL-APLDHE 65
           +R VC +WR S LP    +  L  +FP   PF +++     F  L QS +Y+L APL   
Sbjct: 39  LRSVCATWRLS-LPLSNKNNRLS-KFPKYLPFWSSSSSSSGFFTLKQSNVYKLEAPL--- 98

Query: 66  PSSNVSSSSSPRRNGRLVKLEKSELGKMRFLHPLSRRPIRCNLEDLSEEHNELNLLDLQI 125
                    +PR    LVKL+++  G MR L   S   I C L +     ++++L +  +
Sbjct: 99  ---------NPRT--CLVKLQETTPGIMRVLDLFSNDRI-CFLPE--NFPSKIDLQEFHV 158

Query: 126 DELVISYSLKYTD-----TTCVHGIAKIVVFPNSTGPDVKDCTVIAVFENGKLGFARSG- 185
             +  +Y ++Y +       C   +    V   S+G   +D  +IA+   GKLGF +SG 
Sbjct: 159 RLVRRTYRMEYANNGGGAVPCFWSLHSDKVVILSSG---EDSAIIAIHSGGKLGFLKSGN 218

Query: 186 DEKWTLIDEQ-NFHYDDVIVY-NQQYYTVDRRGTVFWIDSSMRLVQFSPSLV-GLGHHKH 245
           DEKW ++D   N  Y+D+++Y + +   VD +G     D   ++   +  LV G GH KH
Sbjct: 219 DEKWKILDNSWNVIYEDIMLYKDNRCIVVDDKGKTVIYDVDFKVSDLAEGLVGGGGHKKH 278

Query: 246 LVEC-GGELLVIDRFLDKERQFQHPIAVVDDAQHPFAMPVRRPPYDSSARAVDFKVHKLD 305
           LVEC GGE+ ++D++                      +       D S   V+F+V+ L 
Sbjct: 279 LVECSGGEVFLVDKY----------------------VKTVWCKSDISKSVVEFRVYNLK 338

Query: 306 QEWGTWVELKNLRNRSIILGNDCCFSVE--ASEFEGCKENCIYYTDLDDG 339
           +E   W E+++L + ++ +G+DC FSV+  A +  G     I+Y+D  +G
Sbjct: 339 REEKRWEEVRDLGDVALFIGDDCSFSVQNPAGDLAG---GFIFYSDYRNG 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SHX93.5e-2829.29Putative F-box protein At1g65770 OS=Arabidopsis thaliana OX=3702 GN=At1g65770 PE... [more]
Q1PEZ88.6e-2728.32F-box protein At2g26160 OS=Arabidopsis thaliana OX=3702 GN=At2g26160 PE=2 SV=1[more]
Q3EBZ22.8e-2528.17F-box protein SKIP23 OS=Arabidopsis thaliana OX=3702 GN=SKIP23 PE=1 SV=1[more]
Q9LS042.2e-2225.82Putative F-box protein At3g25750 OS=Arabidopsis thaliana OX=3702 GN=At3g25750 PE... [more]
Q9LVG83.8e-2229.43Putative F-box protein At5g60060 OS=Arabidopsis thaliana OX=3702 GN=At5g60060 PE... [more]
Match NameE-valueIdentityDescription
A0A5A7VPR36.6e-17980.94Putative F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A1S3CRG56.6e-17980.94putative F-box protein At1g65770 OS=Cucumis melo OX=3656 GN=LOC103503900 PE=4 SV... [more]
A0A6J1GFH53.4e-17580.95uncharacterized protein LOC111453467 OS=Cucurbita moschata OX=3662 GN=LOC1114534... [more]
A0A6J1IRW41.4e-17380.69putative F-box protein At1g65770 OS=Cucurbita maxima OX=3661 GN=LOC111478154 PE=... [more]
A0A0A0KLM68.3e-16675.58DUF295 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G118170 PE=4... [more]
Match NameE-valueIdentityDescription
XP_038876198.13.7e-19287.07F-box protein At2g26160-like [Benincasa hispida][more]
XP_008466513.11.4e-17880.94PREDICTED: putative F-box protein At1g65770 [Cucumis melo] >KAA0067551.1 putativ... [more]
XP_022950355.17.0e-17580.95uncharacterized protein LOC111453467 [Cucurbita moschata][more]
KAG6603875.11.6e-17480.95putative F-box protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023543888.17.7e-17480.69putative F-box protein At1g65770 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G65770.12.5e-2929.29ascorbic acid mannose pathway regulator 1 [more]
AT2G26160.16.1e-2828.32F-box family protein with a domain of unknown function (DUF295) [more]
AT2G17030.12.0e-2628.17F-box family protein with a domain of unknown function (DUF295) [more]
AT3G25750.12.7e-2324.65F-box family protein with a domain of unknown function (DUF295) [more]
AT5G60060.12.7e-2329.43Protein of unknown function (DUF295) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005174Domain unknown function DUF295PFAMPF03478DUF295coord: 297..351
e-value: 2.8E-13
score: 49.3
NoneNo IPR availablePANTHERPTHR47123F-BOX PROTEIN SKIP23coord: 6..379
NoneNo IPR availablePANTHERPTHR47123:SF6ASCORBIC ACID MANNOSE PATHWAY REGULATOR-RELATEDcoord: 6..379

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G007520.1Lsi09G007520.1mRNA