Lsi09G007420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G007420
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAluminum-activated malate transporter
Locationchr09: 8555280 .. 8557861 (-)
RNA-Seq ExpressionLsi09G007420
SyntenyLsi09G007420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATTCAGGCATTGGCAGCCAAGTTGAAGAAGAAAGCCATGAAAATCAAGCAACTTGGACAAGATGATCCAAGAATAATCATTCACTCCATTAAAGTTGGAATGGCTTTAACTTTGGTCTCATTGTTCTATTATTTGAGGCCTCTTTATGATGATTTTGGGGTCTCTGGGATTTCTGCTGTTATCACAGTTGTGGTTATCTTTGAATTCACTGTTGGTTAGTATAATTTTGCTCTTGGAAAAATTATATATTCTTCAAATTTTGAACCCTTTTTCATTTTTTATTTTATTTTCTTTTTCTTTTGCAGGTGCAACCCTCTGTAAAGGTTTTAATAGAGGTTTGGGAATATTTCTAGCTAGTGCTTTAGCAGTGGGAGTTAACTATTTGGCCAAACTCTCTGGACACAAAGGAGAACCCTTTGTATTGGGCATTTTAGTTTTTCTTATAGGTAACTTTTTTTTTTCTTTTTTTCCTTAATAAACATTTCATATGCACTCAATGTTAGAGGTTTGAATTTATTGAGTTTTAATAAAAAGTTGTTGGTTTTGGATTTTCAGATGTATTAGCCACATTTTTAAGGTTCTTGCCTGTGATCAAAGCAAGATATGATTATGGAGTTTTGATGTTCATAGTGACTTTCACTATAGTTTCGGTTTCGGGTTATTGGATCAATAACTTCTTTACGTTGGCTTATCGGAGACTGTTTGCTGTTCTTGCTGGTGGAGCTATCTGCATTATCATCTCCATTGTTGTTTGCCCTGTTTGGGCTGGTGAAACTCTTCATGATTCTGTTACTTCCAATATAGACAAGTTAGCTAATTACCTTGAAGGTTAGAAAAAATCCCAAAAGAAAAAAGTAATTTATTCACTTGGGATTCTCTTGAATTTAGTGAAGTAATAAAGGTTAAAATTTTACAGGATTTGGTGAAGAATATTTTCATTGTTCTGAAGATGAAGAAGAACATGTTACTGATATAGAAAAAGACAAATCATTCCTTCAAGAATACAAAGTTGTCCTTGATTCTAATACCACAGAAGAATCCTTGGTGGGTTTTCTCTCTAATAAAAACAAGATTTTTAATCTTTCTTTGTCTCTAAATTTTAAAACCACTCTTTGTTTTCTTTTTCTGTTCTTGTTTCTAACAGGAAAACTTTGCAAGATGGGAACCAAAACATGGAAAGTTTGGCTTTCATCACCCATGGAAACAGTACTTGAAAATTGGATCCTTTGCTAGACAATGTGCCTATGGGATTGAAGCTCTCAATGGCTATCTTTCTCCTGAAAATAACTTATACATATCCCAATCAAAAGAGCAATTGCAATTTGGAAGAACAACTGAAGTTCTATGCAAGAACTTAAGTTCAGAATTAGGAAAGACTCTAAAAGCCTTAGCCACAGCAATGAAGACAATGACTGATCCATCACCCTCATCCCAATTTCATCTAAAAGCTGCCAAATCCTCCATTGATGATCTAAAAGAATCCTTCTTCAAATCTGTTGCCACTGCCACAGTGGCTCCCATATTGTATGCGATGGTTAAATCAGTGGAAGGATTGTCTGAGGCAGTGGCTGAACTCTCTCTAAAAGCTCATTTCAAGAGTGTTGAACCCAATGTTTCAGTTTCATCAAACAAGCCTCTGTTGCTTCACAGTGGAATTGTAAATCCATTGTTTGAAGAAACTGAAGATGAACAACCCCATGTTGTTATTATACTGATAGCAACAGAGATTGAGGATTTACCTGAAAAATAAGATGTGAAAGAGCTTCTTTCCTACAGGTATTTGATCTACTAATTCTCTCTGTTTTGAGTTTTATCACATGTTTTAATCAAGTGGGTAGCCATAATTTTAGCCAATGTCAATTGGGTTTCCTTTCATCTGTTCAACTCTCACCTGACCTTTGATTCTCCTTGTGTTGGCTTGATTTGTTTGAAAAAAGTAGTAGTAAAGGATGAGTATGCTGTGATATGTTTTGTTTCATAACAACTTAGTGTATGTGATTTTGCACAGTTTGTTTTCCTTTCAAACTGTTGAAAATGTTGAATGTTTTTGACTTGGGATTTTGTGTCACTGCATTACAAGTTCCCCCCTAATATTGATGGATTACAATGAATTGAATATATTGAACATGGGATCAAGTGAAAATTTTGGATCATTGTGCTTTCTTCTAAGTTGCTGACATGTTATGACAGGAAATTGTGTTGAAACTAGATTCATACCAAAATAAATACATTACTTAAGTGTTACTTTTGGTTTTTCTTTTCTCTAATCTTATATTGCTGATGGGAACAATTCAGGCATTCTATGTTGAAAGTCAAGAAGTAGAAACCGAAACCGTGCTCGATGTCTTACCGGTAAAGGACCTGCTCGATCCTTCGTGTCTTGCCGGACTTGCATTCCCCTTAGAAGAAGAGAGAATGAGGTTGTCTCCTCCTCATGTTTTAACTGTTTTCTTGCTGGTGGAGTGTTGTTGGATTGATTGTTCTGTGATGAATTTGGAGGCTCCATATTTAAGAGAAACCTCTAAGTTCTGCTCAGAATGCTATGTTTAGAAGGTTAGGAATTTGATGTGTTTTGT

mRNA sequence

ATGGCGATTCAGGCATTGGCAGCCAAGTTGAAGAAGAAAGCCATGAAAATCAAGCAACTTGGACAAGATGATCCAAGAATAATCATTCACTCCATTAAAGTTGGAATGGCTTTAACTTTGGTCTCATTGTTCTATTATTTGAGGCCTCTTTATGATGATTTTGGGGTCTCTGGGATTTCTGCTGTTATCACAGTTGTGGTTATCTTTGAATTCACTGTTGGTGCAACCCTCTGTAAAGGTTTTAATAGAGGTTTGGGAATATTTCTAGCTAGTGCTTTAGCAGTGGGAGTTAACTATTTGGCCAAACTCTCTGGACACAAAGGAGAACCCTTTGTATTGGGCATTTTAGTTTTTCTTATAGATGTATTAGCCACATTTTTAAGGTTCTTGCCTGTGATCAAAGCAAGATATGATTATGGAGTTTTGATGTTCATAGTGACTTTCACTATAGTTTCGGTTTCGGGTTATTGGATCAATAACTTCTTTACGTTGGCTTATCGGAGACTGTTTGCTGTTCTTGCTGGTGGAGCTATCTGCATTATCATCTCCATTGTTGTTTGCCCTGTTTGGGCTGGTGAAACTCTTCATGATTCTGTTACTTCCAATATAGACAAGTTAGCTAATTACCTTGAAGGATTTGGTGAAGAATATTTTCATTGTTCTGAAGATGAAGAAGAACATGTTACTGATATAGAAAAAGACAAATCATTCCTTCAAGAATACAAAGTTGTCCTTGATTCTAATACCACAGAAGAATCCTTGGAAAACTTTGCAAGATGGGAACCAAAACATGGAAAGTTTGGCTTTCATCACCCATGGAAACAGTACTTGAAAATTGGATCCTTTGCTAGACAATGTGCCTATGGGATTGAAGCTCTCAATGGCTATCTTTCTCCTGAAAATAACTTATACATATCCCAATCAAAAGAGCAATTGCAATTTGGAAGAACAACTGAAGTTCTATGCAAGAACTTAAGTTCAGAATTAGGAAAGACTCTAAAAGCCTTAGCCACAGCAATGAAGACAATGACTGATCCATCACCCTCATCCCAATTTCATCTAAAAGCTGCCAAATCCTCCATTGATGATCTAAAAGAATCCTTCTTCAAATCTGTTGCCACTGCCACAGTGGCTCCCATATTGTATGCGATGGTTAAATCAGTGGAAGGATTGTCTGAGGCAGTGGCTGAACTCTCTCTAAAAGCTCATTTCAAGAGTGTTGAACCCAATGTTTCAGTTTCATCAAACAAGCCTCTGTTGCTTCACAGTGGAATTGTAAATCCATTGTTTGAAGAAACTGAAGATGAACAACCCCATGTATTTGATCTACTAATTCTCTCTGTTTTGAGTTTTATCACATGTTTTAATCAAGCATTCTATGTTGAAAGTCAAGAAGTAGAAACCGAAACCGTGCTCGATGTCTTACCGGTAAAGGACCTGCTCGATCCTTCGTGTCTTGCCGGACTTGCATTCCCCTTAGAAGAAGAGAGAATGAGGTTGTCTCCTCCTCATGTTTTAACTGTTTTCTTGCTGGTGGAGTGTTGTTGGATTGATTGTTCTGTGATGAATTTGGAGGCTCCATATTTAAGAGAAACCTCTAAGTTCTGCTCAGAATGCTATGTTTAGAAGGTTAGGAATTTGATGTGTTTTGT

Coding sequence (CDS)

ATGGCGATTCAGGCATTGGCAGCCAAGTTGAAGAAGAAAGCCATGAAAATCAAGCAACTTGGACAAGATGATCCAAGAATAATCATTCACTCCATTAAAGTTGGAATGGCTTTAACTTTGGTCTCATTGTTCTATTATTTGAGGCCTCTTTATGATGATTTTGGGGTCTCTGGGATTTCTGCTGTTATCACAGTTGTGGTTATCTTTGAATTCACTGTTGGTGCAACCCTCTGTAAAGGTTTTAATAGAGGTTTGGGAATATTTCTAGCTAGTGCTTTAGCAGTGGGAGTTAACTATTTGGCCAAACTCTCTGGACACAAAGGAGAACCCTTTGTATTGGGCATTTTAGTTTTTCTTATAGATGTATTAGCCACATTTTTAAGGTTCTTGCCTGTGATCAAAGCAAGATATGATTATGGAGTTTTGATGTTCATAGTGACTTTCACTATAGTTTCGGTTTCGGGTTATTGGATCAATAACTTCTTTACGTTGGCTTATCGGAGACTGTTTGCTGTTCTTGCTGGTGGAGCTATCTGCATTATCATCTCCATTGTTGTTTGCCCTGTTTGGGCTGGTGAAACTCTTCATGATTCTGTTACTTCCAATATAGACAAGTTAGCTAATTACCTTGAAGGATTTGGTGAAGAATATTTTCATTGTTCTGAAGATGAAGAAGAACATGTTACTGATATAGAAAAAGACAAATCATTCCTTCAAGAATACAAAGTTGTCCTTGATTCTAATACCACAGAAGAATCCTTGGAAAACTTTGCAAGATGGGAACCAAAACATGGAAAGTTTGGCTTTCATCACCCATGGAAACAGTACTTGAAAATTGGATCCTTTGCTAGACAATGTGCCTATGGGATTGAAGCTCTCAATGGCTATCTTTCTCCTGAAAATAACTTATACATATCCCAATCAAAAGAGCAATTGCAATTTGGAAGAACAACTGAAGTTCTATGCAAGAACTTAAGTTCAGAATTAGGAAAGACTCTAAAAGCCTTAGCCACAGCAATGAAGACAATGACTGATCCATCACCCTCATCCCAATTTCATCTAAAAGCTGCCAAATCCTCCATTGATGATCTAAAAGAATCCTTCTTCAAATCTGTTGCCACTGCCACAGTGGCTCCCATATTGTATGCGATGGTTAAATCAGTGGAAGGATTGTCTGAGGCAGTGGCTGAACTCTCTCTAAAAGCTCATTTCAAGAGTGTTGAACCCAATGTTTCAGTTTCATCAAACAAGCCTCTGTTGCTTCACAGTGGAATTGTAAATCCATTGTTTGAAGAAACTGAAGATGAACAACCCCATGTATTTGATCTACTAATTCTCTCTGTTTTGAGTTTTATCACATGTTTTAATCAAGCATTCTATGTTGAAAGTCAAGAAGTAGAAACCGAAACCGTGCTCGATGTCTTACCGGTAAAGGACCTGCTCGATCCTTCGTGTCTTGCCGGACTTGCATTCCCCTTAGAAGAAGAGAGAATGAGGTTGTCTCCTCCTCATGTTTTAACTGTTTTCTTGCTGGTGGAGTGTTGTTGGATTGATTGTTCTGTGATGAATTTGGAGGCTCCATATTTAAGAGAAACCTCTAAGTTCTGCTCAGAATGCTATGTTTAG

Protein sequence

MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPENNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSSIDDLKESFFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPNVSVSSNKPLLLHSGIVNPLFEETEDEQPHVFDLLILSVLSFITCFNQAFYVESQEVETETVLDVLPVKDLLDPSCLAGLAFPLEEERMRLSPPHVLTVFLLVECCWIDCSVMNLEAPYLRETSKFCSECYV
Homology
BLAST of Lsi09G007420 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.2e-99
Identity = 205/451 (45.45%), Postives = 281/451 (62.31%), Query Frame = 0

Query: 3   IQALAAKLK----KKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSG 62
           +Q   +KLK    K+   +++  +DDPR IIHS+KVG+ALTLVSL YY+RPLY  FGV+G
Sbjct: 16  LQDFPSKLKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTG 75

Query: 63  ISAVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVF 122
           + A++TVVV+FEFTVG TL KG NRG    +A AL VG  +LA+  GH+GEP VLGILVF
Sbjct: 76  MWAILTVVVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVF 135

Query: 123 LIDVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAI 182
            +   ATF RF P IK RYDYG L+FI+TF+ V++SGY  +    +AY+RL  +L GG I
Sbjct: 136 SLGAAATFSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTI 195

Query: 183 CIIISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFL 242
           CI++SI +CPVWAGE LH  + +NI+KLA YLEGF  EYF   +  +E         S +
Sbjct: 196 CILVSIFICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKISKE-------TSSCV 255

Query: 243 QEYKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLS 302
           +EYK +L S +TE+SL N ARWEP HG+F   HPWK+YLKI    RQCA  +E LNGY+ 
Sbjct: 256 REYKSILTSKSTEDSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYV- 315

Query: 303 PENNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAK 362
                 +S  K   +F    +     +S E+G+ LKA+A ++KTM + S     H+  +K
Sbjct: 316 ------LSNDKAPQEFESKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSK 375

Query: 363 SSIDDLK-----------ESFFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFK-S 422
            +I +LK           +   + +   T+A IL  +V  VE + EAV E S  AHFK +
Sbjct: 376 KAIKNLKIALKSSYPETYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKET 435

Query: 423 VEPNVSVSSNKPLLLHSGIVNPLFEETEDEQ 438
           ++  +S    +  LLH G V P+ +   +++
Sbjct: 436 LDSKLSAEIGQHQLLHRGCVKPVLDGDNEKE 452

BLAST of Lsi09G007420 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 1.0e-88
Identity = 183/447 (40.94%), Postives = 282/447 (63.09%), Query Frame = 0

Query: 9   KLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAVITVVVI 68
           K+++   + +++G++DPR ++H+ KVG+AL LVS FYY +PLYD+FGV+ + AV+TVVV+
Sbjct: 3   KVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVV 62

Query: 69  FEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLR 128
           FEF+VGATL KG NR +   +A  L +G ++LA LSG   EP +L I VF++  L+TF+R
Sbjct: 63  FEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVR 122

Query: 129 FLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCP 188
           F P +KARYDYGVL+FI+TF ++SVSG+  +    LA++RL  V+ GG  C++ISI VCP
Sbjct: 123 FFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCP 182

Query: 189 VWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYKVVLDSN 248
           VWAG+ LH  + SN D L+++L+ FG+EYF  +ED +  + ++EK +  L+ YK VL+S 
Sbjct: 183 VWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGD--IKEVEKRRRNLERYKSVLNSK 242

Query: 249 TTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPENNLYISQS 308
           + EE+L NFA+WEP+HG+F F HPW+QYL +G+  RQ AY I+ALN  ++ +  + +   
Sbjct: 243 SNEEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIK 302

Query: 309 KEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSSIDDLKESF 368
           K+        E   + +SSE GK++K ++ ++K MT  S S   H+  ++S+   L    
Sbjct: 303 KK-------IEEPLRRMSSESGKSMKEVSISLKNMT-ISSSFDIHVVNSQSACKTLSTLL 362

Query: 369 ----------FKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPNVSVSSNKP 428
                      + ++  T   +L  +V   E +SE+V EL+  A FK        +  KP
Sbjct: 363 KSGILNDVEPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK--------NKKKP 422

Query: 429 LLLHSGIVNPLF-EETEDEQPHVFDLL 445
              +SG +      ++ D+  HV  +L
Sbjct: 423 SKSNSGSIGQAMPNKSHDDDDHVVTIL 431

BLAST of Lsi09G007420 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.3e-82
Identity = 183/440 (41.59%), Postives = 266/440 (60.45%), Query Frame = 0

Query: 19  QLGQDDPRIIIHSIKVGMALTLVSLFYYLR---PLYDDFGVSGISAVITVVVIFEFTVGA 78
           ++G +DPR IIH+ KVG+AL LVS FYY +   P  D FG++ + AV+TVVV+FEF+VGA
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 79  TLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLRFLPVIKA 138
           TL KG NRG+   +A  L +G + LA+LSG   EP +L +LVF+   L+TF+RF P +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 139 RYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCPVWAGETL 198
           ++DYG+L+FI+TF ++S+SG+       LA  RL  V+ GG  CI+ISI VCPVWAG+ L
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 199 HDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYKVVLDSNTTEESLE 258
           H  + SN D L+++L+ FG+EYF   E  +  V  +EK K  L+ YK VLDS + EE+L 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKV--VEKRKKNLERYKSVLDSKSDEEALA 252

Query: 259 NFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPENNLYISQSKEQLQFG 318
           N+A WEP HG+F F HPWKQY+ +G+  RQCAY I+ALN Y++ +  + +   K+     
Sbjct: 253 NYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKK----- 312

Query: 319 RTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHL---KAAKSSIDDLKESF---- 378
              E   + +SSE G ++K ++ ++K M   S SS  H+   +AA  S+  L +S     
Sbjct: 313 --LETPLRRMSSESGNSMKEMSISLKQMI-KSSSSDIHVSNSQAACKSLSTLLKSGILND 372

Query: 379 ---FKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKS-VEPNVSVSSNKPLLLHSG 438
               + ++  T   +L  +V   E +SE+V EL+  A FK+ + P V    +      SG
Sbjct: 373 VEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLYEKS-----DSG 432

Query: 439 IVNPLFEETEDEQPHVFDLL 445
            +         E  HV  +L
Sbjct: 433 SIGRAMPIDSHEDHHVVTVL 437

BLAST of Lsi09G007420 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.2e-81
Identity = 176/434 (40.55%), Postives = 256/434 (58.99%), Query Frame = 0

Query: 9   KLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAVITVVVI 68
           K+++   + +++ ++DPR ++HS KVG+ L LVS FYY +PLYD FGV+ + AV+TVVV+
Sbjct: 3   KVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVV 62

Query: 69  FEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLR 128
           FEF+VGATL KG NR      A  L +G ++LA +SG  GEP +L + VF+   L+TF+R
Sbjct: 63  FEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVR 122

Query: 129 FLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCP 188
           F P +KARYDY +L+FI+TF ++SVSG+       L ++R+  V+ GG  C+IISI VCP
Sbjct: 123 FFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCP 182

Query: 189 VWAGETLHDSVTSNIDKLANYLEG--------------------------FGEEYFHCSE 248
           VWAG+ LH  + SN +KL+ +L G                          FG++Y  C  
Sbjct: 183 VWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKY--CEV 242

Query: 249 DEEEHVTDIEKDKSFLQEYKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSF 308
            E +   +++K K     YK VL+S + EESL NFA+WEP HG+F F HPWKQYL +G  
Sbjct: 243 VENDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGEL 302

Query: 309 ARQCAYGIEALNGYLSPENNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKT 368
            RQCAY I ALN YL+ +N + +   K      +  E L + +S E GK +K ++ ++K 
Sbjct: 303 IRQCAYRIHALNSYLNADNQVSVDIKK------KLGEPL-RRMSLESGKAMKEMSISLKK 362

Query: 369 MTDPSPSSQFHLKAAKSSIDDLKESF----------FKSVATATVAPILYAMVKSVEGLS 407
           MT PS SS  H++ AKS+   L               + V+  T   +L  ++   E + 
Sbjct: 363 MTKPS-SSDLHVQNAKSACKSLTNLLNSGILKEVEPLELVSLLTAISLLIDIINLTEKIL 422

BLAST of Lsi09G007420 vs. ExPASy Swiss-Prot
Match: Q76LB1 (Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 7.0e-77
Identity = 183/432 (42.36%), Postives = 258/432 (59.72%), Query Frame = 0

Query: 14  AMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAVITVVVIFEFTV 73
           A K+    ++DPR + HS+KVG+AL LVS+ Y++ PL++  GVS I AV+TVVV+ E+TV
Sbjct: 39  ARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGVSAIWAVLTVVVVMEYTV 98

Query: 74  GATLCKGFNRGLGIFLASALAVGVNYLAKLS---GHKGEPFVLGILVFLIDVLATFLRFL 133
           GATL KG NR L   +A  +AVG + LA+L+   G +GEP VL +LVF +   ATFLRF+
Sbjct: 99  GATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVLTVLVFFVASAATFLRFI 158

Query: 134 PVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCPVW 193
           P IKA+YDYGV +FI+TF +V+VS Y +     LA++R + +  G  IC+  ++ + PVW
Sbjct: 159 PEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIAVGVFICLCTTVFLFPVW 218

Query: 194 AGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYKVVLDSNTT 253
           AGE +H   + N+DKLA ++EG     F+C    E  V +    K   Q +K VL+S  T
Sbjct: 219 AGEDVHKLASGNLDKLAQFIEGM---EFNCF--GENSVANNFGGKDSPQMHKSVLNSKAT 278

Query: 254 EESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPENNLYISQSKE 313
           E+SL  FA+WEP+HG+F F HPW QY K+G+  RQCA  +EAL  Y+       I+ SK 
Sbjct: 279 EDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALASYV-------ITTSKT 338

Query: 314 QLQFGRTTEVLCK------NLSSELGKTLKALATAMKTMTDPSP---SSQFHLKAAKSSI 373
           Q       E+ CK       +S    K L+ LA A +TMT PSP   +    +KAA+S  
Sbjct: 339 QCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMATAVKAAESLR 398

Query: 374 DDLKE--SFFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVE--PNVSVSSNK 430
            +L E  +  + +  A  A +L  +V  V+ ++E V  L+  AHFK+ E   NV VS+  
Sbjct: 399 SELAENTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHFKNPEDTKNVVVST-- 453

BLAST of Lsi09G007420 vs. ExPASy TrEMBL
Match: A0A5D3DNX6 (Aluminum-activated malate transporter 8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004590 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 8.2e-153
Identity = 310/457 (67.83%), Postives = 346/457 (75.71%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ L  K  + A+KIK+LGQDDPR IIHSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQ-LKNKAMEVALKIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AVITVVVIFEFTVGATL KG NRGLG  LA AL VGV+YLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVITVVVIFEFTVGATLSKGLNRGLGTMLAGALGVGVDYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VSVSGY ++ F T+A++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSMVSVSGYRVDEFLTMAHQRLTTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           IISIVVCPVWAGETLH+S+ SNI+KLANYLEGFG EYFH S   +EHVT  EKDKSFLQE
Sbjct: 181 IISIVVCPVWAGETLHNSIISNINKLANYLEGFGGEYFHSS---DEHVTVPEKDKSFLQE 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YKVVL+S +TE+S+ NFARWEP+HG FGF HPWK YLKIGSFARQCAY IEALN +LSP 
Sbjct: 241 YKVVLNSKSTEDSMANFARWEPRHGNFGFRHPWKHYLKIGSFARQCAYHIEALNFHLSPH 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q +E  QF R  EV CK +SSE GK LKALATAMKTMTDPSPSSQ HL AAKS+
Sbjct: 301 ------QLQEPSQFRRMMEVPCKTISSESGKALKALATAMKTMTDPSPSSQLHLNAAKSA 360

Query: 361 IDDLKESFFKSVA--------------TATVAPILYAMVKSVEGLSEAVAELSLKAHFKS 420
           ++DLK +     A               ATVA IL  +VKSVE LSEAVAELSLKA FKS
Sbjct: 361 VNDLKNTLKSGTAQISDDISNLLAIIPDATVASILIDIVKSVEDLSEAVAELSLKAKFKS 420

Query: 421 VEPNVSVSSNKPLLLHSGIVNPLFEETEDE---QPHV 441
           V P       KP LLH G + P  EE ++E   QPHV
Sbjct: 421 VSP------EKPQLLHKGTIKPFVEEDDNEAQQQPHV 441

BLAST of Lsi09G007420 vs. ExPASy TrEMBL
Match: A0A1S3B7M1 (aluminum-activated malate transporter 8-like OS=Cucumis melo OX=3656 GN=LOC103486680 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 8.2e-153
Identity = 310/457 (67.83%), Postives = 346/457 (75.71%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ L  K  + A+KIK+LGQDDPR IIHSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQ-LKNKAMEVALKIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AVITVVVIFEFTVGATL KG NRGLG  LA AL VGV+YLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVITVVVIFEFTVGATLSKGLNRGLGTMLAGALGVGVDYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VSVSGY ++ F T+A++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSMVSVSGYRVDEFLTMAHQRLTTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           IISIVVCPVWAGETLH+S+ SNI+KLANYLEGFG EYFH S   +EHVT  EKDKSFLQE
Sbjct: 181 IISIVVCPVWAGETLHNSIISNINKLANYLEGFGGEYFHSS---DEHVTVPEKDKSFLQE 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YKVVL+S +TE+S+ NFARWEP+HG FGF HPWK YLKIGSFARQCAY IEALN +LSP 
Sbjct: 241 YKVVLNSKSTEDSMANFARWEPRHGNFGFRHPWKHYLKIGSFARQCAYHIEALNFHLSPH 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q +E  QF R  EV CK +SSE GK LKALATAMKTMTDPSPSSQ HL AAKS+
Sbjct: 301 ------QLQEPSQFRRMMEVPCKTISSESGKALKALATAMKTMTDPSPSSQLHLNAAKSA 360

Query: 361 IDDLKESFFKSVA--------------TATVAPILYAMVKSVEGLSEAVAELSLKAHFKS 420
           ++DLK +     A               ATVA IL  +VKSVE LSEAVAELSLKA FKS
Sbjct: 361 VNDLKNTLKSGTAQISDDISNLLAIIPDATVASILIDIVKSVEDLSEAVAELSLKAKFKS 420

Query: 421 VEPNVSVSSNKPLLLHSGIVNPLFEETEDE---QPHV 441
           V P       KP LLH G + P  EE ++E   QPHV
Sbjct: 421 VSP------EKPQLLHKGTIKPFVEEDDNEAQQQPHV 441

BLAST of Lsi09G007420 vs. ExPASy TrEMBL
Match: A0A6J1J497 (aluminum-activated malate transporter 8-like OS=Cucurbita maxima OX=3661 GN=LOC111481634 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.9e-149
Identity = 299/450 (66.44%), Postives = 344/450 (76.44%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M I+A+  K  + AMKIK+LGQDDPR IIHSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIEAVKNKAMEIAMKIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AV+TVVVIFEFTVGATL KG NRGLG  LA +L VGVNYLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVMTVVVIFEFTVGATLSKGLNRGLGTLLAGSLGVGVNYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VS+SGY ++ F TLA++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSLVSISGYRMDEFLTLAHQRLSTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           +ISI+V PVWAGETLH+SVTSNI+KLA+YLEGFG EYF+C ED  EH    EKDKSF++ 
Sbjct: 181 MISILVFPVWAGETLHNSVTSNINKLADYLEGFGGEYFYCCED-GEHGVATEKDKSFVEG 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YK VL+S + E+S+ NFARWEPKHGKF F HPWK YLKIGS  R CAY IEALN +L P+
Sbjct: 241 YKAVLNSKSMEDSMANFARWEPKHGKFRFRHPWKHYLKIGSLTRHCAYQIEALNRHLCPQ 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q+++  Q  R  EV CK LSSE GK LKALAT MK MT+PSPSSQ HL AAKS+
Sbjct: 301 -----LQTQDPSQLRRMIEVPCKTLSSESGKALKALATGMKAMTNPSPSSQVHLNAAKSA 360

Query: 361 IDDLKESFFKSVA----------TATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPN 420
           ++DLK+S   + A           ATVA IL  +VKSVE LSEAV ELSLKAHFKS+E  
Sbjct: 361 MNDLKDSLKLTTAQTTNLLAIIPDATVASILIEIVKSVEDLSEAVVELSLKAHFKSIE-- 420

Query: 421 VSVSSNKPLLLHSGIVNPLFEETEDEQPHV 441
            +VS  KP LLH G +NP F E EDEQPHV
Sbjct: 421 ATVSPEKPQLLHKGTINP-FVEAEDEQPHV 441

BLAST of Lsi09G007420 vs. ExPASy TrEMBL
Match: A0A6J1FAA4 (aluminum-activated malate transporter 8-like OS=Cucurbita moschata OX=3662 GN=LOC111442257 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 4.2e-149
Identity = 297/453 (65.56%), Postives = 343/453 (75.72%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ +  K  + AMKIK+LGQDDPR I+HSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQVVKNKAMEIAMKIKKLGQDDPRRIVHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AV+TVVVIFEFTVG TL KG NRGLG  LA +L VGVNYLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVMTVVVIFEFTVGGTLSKGLNRGLGTLLAGSLGVGVNYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VS+SGY I+ F TLA++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSLVSISGYRIDEFLTLAHQRLSTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           +ISI+VCPVWAGETLH+SVTSNI+KLA+YLEGFG EYF+C ED  EH    EK KSF++ 
Sbjct: 181 MISILVCPVWAGETLHNSVTSNINKLADYLEGFGGEYFYCCED-GEHGVATEKGKSFVEG 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YK VL+S + E+S+ NFARWEPKHGKF F HPWK YLKIGS  RQCAY IEALN +L P+
Sbjct: 241 YKAVLNSKSMEDSMANFARWEPKHGKFRFRHPWKHYLKIGSLTRQCAYQIEALNRHLCPQ 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q+++  Q  R  EV CK LSSE GK LKALAT MK MT+PS SSQ HL AAKS+
Sbjct: 301 -----LQTQDPSQLRRMIEVPCKTLSSESGKALKALATGMKAMTNPSSSSQVHLNAAKSA 360

Query: 361 IDDLKES----------FFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPN 420
           ++DLK+S            + +  ATVA IL  +VKSVE LSEAV ELSLKAHFKS+E  
Sbjct: 361 MNDLKDSLKLGTAQTTNLLEIIPDATVASILIEIVKSVEDLSEAVVELSLKAHFKSIE-- 420

Query: 421 VSVSSNKPLLLHSGIVNPLFEETEDEQPHVFDL 444
            +VS  KP LLH G +NPL  E EDEQPHV  +
Sbjct: 421 ATVSPEKPQLLHKGTINPLV-EAEDEQPHVISV 444

BLAST of Lsi09G007420 vs. ExPASy TrEMBL
Match: A0A6J1DEM9 (aluminum-activated malate transporter 8-like OS=Momordica charantia OX=3673 GN=LOC111019370 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 9.3e-149
Identity = 300/459 (65.36%), Postives = 342/459 (74.51%), Query Frame = 0

Query: 3   IQALAAKLKKK----AMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSG 62
           I+    KLK K    AM+IK+LGQDDPR IIHSIKVG+ALTLVSLFYY RPLYD FG SG
Sbjct: 4   IKTAPTKLKNKAMEVAMRIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWRPLYDGFGASG 63

Query: 63  ISAVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVF 122
           I AVITVVVIFEFTVGATL KG NRGLG  LA AL VGV++LA LSGHKGEP VLGI VF
Sbjct: 64  IWAVITVVVIFEFTVGATLSKGLNRGLGTLLAGALGVGVDHLANLSGHKGEPIVLGIFVF 123

Query: 123 LIDVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAI 182
           LI   ATF RF P IKARYDYGVL+FI+TF++VSVSGY ++ F T+A +RL  +LAGGAI
Sbjct: 124 LIAAAATFSRFFPGIKARYDYGVLIFILTFSMVSVSGYRVDEFVTMAQQRLSTILAGGAI 183

Query: 183 CIIISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFL 242
           CI+ISI++CPVWAGETLH+SV SNIDKLANYLEGFG EYFHC EDE++ VT  EKDKSFL
Sbjct: 184 CIMISILICPVWAGETLHNSVASNIDKLANYLEGFGGEYFHCFEDEDQQVTAKEKDKSFL 243

Query: 243 QEYKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLS 302
           Q YK VL+S + E+S+ NFARWEP+HG F F HPWKQYLKIGSFARQCAY IEALN +LS
Sbjct: 244 QGYKTVLNSKSMEDSMANFARWEPRHGNFNFRHPWKQYLKIGSFARQCAYHIEALNSHLS 303

Query: 303 PENNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAK 362
           P       + +   +F R  E  CK LSSE GK LKALATAMKTM DPS SS  HL AAK
Sbjct: 304 P-------RLQAPSEFRRMIEAPCKALSSESGKALKALATAMKTMADPSSSSHRHLNAAK 363

Query: 363 SSIDDLKESFFKSVA-----------------TATVAPILYAMVKSVEGLSEAVAELSLK 422
           S+I+DLK++   + A                  ATVA  L  +VKSVE LSE+V ELS+K
Sbjct: 364 SAINDLKDALKSATALISNDPSSSNDLLAIIPDATVACKLIEIVKSVENLSESVGELSVK 423

Query: 423 AHFKSVEPNVSVSSNKPLLLHSGIVNPLFEETEDEQPHV 441
           AHFK VE   +VS  KP LLH G + P+  E E+E PHV
Sbjct: 424 AHFKKVE--ATVSPEKPQLLHKGTIKPV-AEVEEELPHV 452

BLAST of Lsi09G007420 vs. NCBI nr
Match: XP_008442919.1 (PREDICTED: aluminum-activated malate transporter 8-like [Cucumis melo] >KAA0043811.1 aluminum-activated malate transporter 8-like [Cucumis melo var. makuwa] >TYK25321.1 aluminum-activated malate transporter 8-like [Cucumis melo var. makuwa])

HSP 1 Score: 550.4 bits (1417), Expect = 1.7e-152
Identity = 310/457 (67.83%), Postives = 346/457 (75.71%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ L  K  + A+KIK+LGQDDPR IIHSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQ-LKNKAMEVALKIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AVITVVVIFEFTVGATL KG NRGLG  LA AL VGV+YLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVITVVVIFEFTVGATLSKGLNRGLGTMLAGALGVGVDYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VSVSGY ++ F T+A++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSMVSVSGYRVDEFLTMAHQRLTTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           IISIVVCPVWAGETLH+S+ SNI+KLANYLEGFG EYFH S   +EHVT  EKDKSFLQE
Sbjct: 181 IISIVVCPVWAGETLHNSIISNINKLANYLEGFGGEYFHSS---DEHVTVPEKDKSFLQE 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YKVVL+S +TE+S+ NFARWEP+HG FGF HPWK YLKIGSFARQCAY IEALN +LSP 
Sbjct: 241 YKVVLNSKSTEDSMANFARWEPRHGNFGFRHPWKHYLKIGSFARQCAYHIEALNFHLSPH 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q +E  QF R  EV CK +SSE GK LKALATAMKTMTDPSPSSQ HL AAKS+
Sbjct: 301 ------QLQEPSQFRRMMEVPCKTISSESGKALKALATAMKTMTDPSPSSQLHLNAAKSA 360

Query: 361 IDDLKESFFKSVA--------------TATVAPILYAMVKSVEGLSEAVAELSLKAHFKS 420
           ++DLK +     A               ATVA IL  +VKSVE LSEAVAELSLKA FKS
Sbjct: 361 VNDLKNTLKSGTAQISDDISNLLAIIPDATVASILIDIVKSVEDLSEAVAELSLKAKFKS 420

Query: 421 VEPNVSVSSNKPLLLHSGIVNPLFEETEDE---QPHV 441
           V P       KP LLH G + P  EE ++E   QPHV
Sbjct: 421 VSP------EKPQLLHKGTIKPFVEEDDNEAQQQPHV 441

BLAST of Lsi09G007420 vs. NCBI nr
Match: XP_031737867.1 (aluminum-activated malate transporter 8 [Cucumis sativus] >KAE8651115.1 hypothetical protein Csa_001300 [Cucumis sativus])

HSP 1 Score: 543.1 bits (1398), Expect = 2.7e-150
Identity = 305/458 (66.59%), Postives = 342/458 (74.67%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ L  K    AMKIK+LGQDDPR IIHSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQ-LKNKAMDVAMKIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AVITVVVIFEFTVGATL KG NRGLG  LA AL VGV+YLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVITVVVIFEFTVGATLSKGLNRGLGTMLAGALGVGVDYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VSVSGY ++ F T+A++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSMVSVSGYRVDEFLTMAHQRLATILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           I+SIVVCPVWAGETLH+S+ SNI+KLANYLEGFG EYFHCS   +EHVT  EKDK FLQE
Sbjct: 181 IVSIVVCPVWAGETLHNSIISNINKLANYLEGFGGEYFHCS---DEHVTIPEKDKPFLQE 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YKVVL+S +TE+S+ NFARWEP+HG FGF HPWK YLKIGS ARQCAY IEALN +LSP 
Sbjct: 241 YKVVLNSKSTEDSMANFARWEPRHGNFGFRHPWKHYLKIGSVARQCAYHIEALNFHLSPH 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q +E  QF R  EV CK +SSE GK LKALATAMK MTDPSPSSQ HL AAKS+
Sbjct: 301 ------QLQEPSQFRRMLEVPCKTISSESGKALKALATAMKKMTDPSPSSQLHLNAAKSA 360

Query: 361 IDDLK--------------ESFFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKS 420
           ++DLK               +    +  ATVA IL  +VKSVE LSEAVAELSLKA FK 
Sbjct: 361 VNDLKNTLKSGTTQISDDISNLLAIIPDATVASILIDIVKSVEDLSEAVAELSLKAKFKR 420

Query: 421 VEPNVSVSSNKPLLLHSGIVNPLFEETED----EQPHV 441
           V P       KP LLH G + P  EE ++    +QPHV
Sbjct: 421 VSP------EKPQLLHKGTIKPFVEEDDNVEAQQQPHV 442

BLAST of Lsi09G007420 vs. NCBI nr
Match: XP_023526244.1 (aluminum-activated malate transporter 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 542.0 bits (1395), Expect = 6.0e-150
Identity = 299/450 (66.44%), Postives = 343/450 (76.22%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ +  K  + AMKIK+LGQDDPR IIHSIKVG+ALTLVSLFYY  PLYD FG SGI 
Sbjct: 1   MGIQVVKNKAMEIAMKIKKLGQDDPRRIIHSIKVGVALTLVSLFYYWNPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AV+TVVVIFEFTVGATL KG NRGLG  LA +L VGVNYLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVMTVVVIFEFTVGATLSKGLNRGLGTLLAGSLGVGVNYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VS+SGY I+ F TLA++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSLVSISGYRIDEFLTLAHQRLSTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           +ISI+VCPVWAGETLH+SVTSNI+KLA+YLEGFG EYF+C ED  EH    EK KSF++ 
Sbjct: 181 MISILVCPVWAGETLHNSVTSNINKLADYLEGFGGEYFYCCED-GEHGVATEKGKSFVEG 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YK VL+S + E+S+ NFARWEPKHGKF F HPWK YLKIGS  RQCAY IEALN +L P+
Sbjct: 241 YKAVLNSKSMEDSMANFARWEPKHGKFRFRHPWKHYLKIGSLTRQCAYQIEALNRHLCPQ 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q+++  Q  R  EV CK LSSE GK LKALAT MK MT+PSPSSQ HL AAKS+
Sbjct: 301 -----IQTQDPSQLRRMIEVPCKTLSSESGKALKALATGMKAMTNPSPSSQVHLNAAKSA 360

Query: 361 IDDLKE----------SFFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPN 420
           ++DLK+          +  + +  ATVA IL  +VKSVE LSEAV ELSLKAHFKS+E  
Sbjct: 361 MNDLKDTLKLGTAQTTNLLEIIPDATVASILIEIVKSVEDLSEAVVELSLKAHFKSIE-- 420

Query: 421 VSVSSNKPLLLHSGIVNPLFEETEDEQPHV 441
            +VS  KP LLH G +NP F E EDEQPHV
Sbjct: 421 ATVSPEKPQLLHKGTINP-FVEAEDEQPHV 441

BLAST of Lsi09G007420 vs. NCBI nr
Match: KAG6580748.1 (Aluminum-activated malate transporter 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 540.4 bits (1391), Expect = 1.7e-149
Identity = 298/450 (66.22%), Postives = 343/450 (76.22%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ +  K  + AMKIK+LGQDDPR I+HSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQVVKNKAMEIAMKIKKLGQDDPRRIVHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AV+TVVVIFEFTVGATL KG NRGLG  LA +L VGVNYLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVMTVVVIFEFTVGATLSKGLNRGLGTLLAGSLGVGVNYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VS+SGY I+ F TLA++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSLVSISGYRIDEFLTLAHQRLSTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           +ISI+VCPVWAGETLH+SVTSNI+KLA+YLEGFG EYF+C ED  EH    EK KSF++ 
Sbjct: 181 MISILVCPVWAGETLHNSVTSNINKLADYLEGFGGEYFYCCED-GEHGVATEKGKSFVEG 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YK VL+S + E+S+ NFARWEPKHGKF F HPWK YLKIGS  RQCAY IEALN +L P+
Sbjct: 241 YKAVLNSKSMEDSMANFARWEPKHGKFRFRHPWKHYLKIGSLTRQCAYQIEALNRHLCPQ 300

Query: 301 NNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSS 360
                 Q+++  Q  R  EV CK LSSE GK LKALAT MK MT+PS SSQ HL AAKS+
Sbjct: 301 -----LQTQDPSQLRRMIEVPCKTLSSESGKALKALATGMKAMTNPSSSSQVHLNAAKSA 360

Query: 361 IDDLKES----------FFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPN 420
           ++DLK+S            + +  ATVA IL  +VKSVE LSEAV ELSLKAHFKS+E  
Sbjct: 361 MNDLKDSLKLGTAQTTNLLEIIPDATVASILIEIVKSVEDLSEAVVELSLKAHFKSIE-- 420

Query: 421 VSVSSNKPLLLHSGIVNPLFEETEDEQPHV 441
            +VS  KP LLH G +NP F E EDEQPHV
Sbjct: 421 ATVSPEKPQLLHKGTINP-FVEAEDEQPHV 441

BLAST of Lsi09G007420 vs. NCBI nr
Match: KAG7017502.1 (Aluminum-activated malate transporter 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.0 bits (1390), Expect = 2.3e-149
Identity = 301/465 (64.73%), Postives = 348/465 (74.84%), Query Frame = 0

Query: 1   MAIQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGIS 60
           M IQ +  K  + AMKIK+LGQDDPR I+HSIKVG+ALTLVSLFYY +PLYD FG SGI 
Sbjct: 1   MGIQVVKNKATEIAMKIKKLGQDDPRRIVHSIKVGVALTLVSLFYYWKPLYDGFGASGIW 60

Query: 61  AVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLI 120
           AV+TVVVIFEFTVGATL KG NRGLG  LA +L VGVNYLA LSG KGEPFVLGI VFLI
Sbjct: 61  AVMTVVVIFEFTVGATLSKGLNRGLGTLLAGSLGVGVNYLANLSGQKGEPFVLGIFVFLI 120

Query: 121 DVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICI 180
              ATF RF P IKARYDYGVL+FI+TF++VS+SGY I+ F TLA++RL  +L GGAICI
Sbjct: 121 AASATFSRFFPGIKARYDYGVLIFILTFSLVSISGYRIDEFLTLAHQRLSTILVGGAICI 180

Query: 181 IISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQE 240
           +ISI+VCPVWAGETLH+SVTSNI+KLA+YLEGFG EYF+C ED  EH    EK KSF++ 
Sbjct: 181 MISILVCPVWAGETLHNSVTSNINKLADYLEGFGGEYFYCCED-GEHGVATEKGKSFVEG 240

Query: 241 YKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPE 300
           YK VL+S + E+S+ NFARWEPKHGKF F HPWK YLKIGS  RQCAY IEALN +L P+
Sbjct: 241 YKAVLNSKSMEDSMANFARWEPKHGKFRFRHPWKHYLKIGSLTRQCAYQIEALNRHLCPQ 300

Query: 301 --------------NNLYI-SQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTD 360
                         + +YI SQ+++  Q  R  EV CK LSSE GK LKALAT MK MT+
Sbjct: 301 LQFIKECNLTLLTNSEIYIVSQTQDPSQLRRMIEVPCKTLSSESGKALKALATGMKAMTN 360

Query: 361 PSPSSQFHLKAAKSSIDDLKES----------FFKSVATATVAPILYAMVKSVEGLSEAV 420
           PS SSQ HL AAKS+++DLK+S            + +  ATVA IL  +VKSVE LSEAV
Sbjct: 361 PSSSSQVHLNAAKSAMNDLKDSLKLGTAQTTNLLEVIPDATVASILIEIVKSVEDLSEAV 420

Query: 421 AELSLKAHFKSVEPNVSVSSNKPLLLHSGIVNPLFEETEDEQPHV 441
            ELSLKAHFKS+E   +VS  KP LLH G +NP F E EDEQPHV
Sbjct: 421 VELSLKAHFKSIE--ATVSPEKPQLLHKGTINP-FVEAEDEQPHV 461

BLAST of Lsi09G007420 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.6e-100
Identity = 205/451 (45.45%), Postives = 281/451 (62.31%), Query Frame = 0

Query: 3   IQALAAKLK----KKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSG 62
           +Q   +KLK    K+   +++  +DDPR IIHS+KVG+ALTLVSL YY+RPLY  FGV+G
Sbjct: 16  LQDFPSKLKDDVTKRVKNVQKFAKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTG 75

Query: 63  ISAVITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVF 122
           + A++TVVV+FEFTVG TL KG NRG    +A AL VG  +LA+  GH+GEP VLGILVF
Sbjct: 76  MWAILTVVVVFEFTVGGTLSKGLNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVF 135

Query: 123 LIDVLATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAI 182
            +   ATF RF P IK RYDYG L+FI+TF+ V++SGY  +    +AY+RL  +L GG I
Sbjct: 136 SLGAAATFSRFFPRIKQRYDYGALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTI 195

Query: 183 CIIISIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFL 242
           CI++SI +CPVWAGE LH  + +NI+KLA YLEGF  EYF   +  +E         S +
Sbjct: 196 CILVSIFICPVWAGEDLHKMIANNINKLAKYLEGFEGEYFQPEKISKE-------TSSCV 255

Query: 243 QEYKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLS 302
           +EYK +L S +TE+SL N ARWEP HG+F   HPWK+YLKI    RQCA  +E LNGY+ 
Sbjct: 256 REYKSILTSKSTEDSLANLARWEPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYV- 315

Query: 303 PENNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAK 362
                 +S  K   +F    +     +S E+G+ LKA+A ++KTM + S     H+  +K
Sbjct: 316 ------LSNDKAPQEFESKIQEPITTMSREVGEALKAIAKSIKTMRNDSACVNAHIDNSK 375

Query: 363 SSIDDLK-----------ESFFKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFK-S 422
            +I +LK           +   + +   T+A IL  +V  VE + EAV E S  AHFK +
Sbjct: 376 KAIKNLKIALKSSYPETYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKET 435

Query: 423 VEPNVSVSSNKPLLLHSGIVNPLFEETEDEQ 438
           ++  +S    +  LLH G V P+ +   +++
Sbjct: 436 LDSKLSAEIGQHQLLHRGCVKPVLDGDNEKE 452

BLAST of Lsi09G007420 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 328.9 bits (842), Expect = 7.4e-90
Identity = 183/447 (40.94%), Postives = 282/447 (63.09%), Query Frame = 0

Query: 9   KLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAVITVVVI 68
           K+++   + +++G++DPR ++H+ KVG+AL LVS FYY +PLYD+FGV+ + AV+TVVV+
Sbjct: 3   KVREIVREGRRVGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVV 62

Query: 69  FEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLR 128
           FEF+VGATL KG NR +   +A  L +G ++LA LSG   EP +L I VF++  L+TF+R
Sbjct: 63  FEFSVGATLGKGLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVR 122

Query: 129 FLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCP 188
           F P +KARYDYGVL+FI+TF ++SVSG+  +    LA++RL  V+ GG  C++ISI VCP
Sbjct: 123 FFPRVKARYDYGVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCP 182

Query: 189 VWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYKVVLDSN 248
           VWAG+ LH  + SN D L+++L+ FG+EYF  +ED +  + ++EK +  L+ YK VL+S 
Sbjct: 183 VWAGQDLHSLLASNFDTLSHFLQEFGDEYFEATEDGD--IKEVEKRRRNLERYKSVLNSK 242

Query: 249 TTEESLENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPENNLYISQS 308
           + EE+L NFA+WEP+HG+F F HPW+QYL +G+  RQ AY I+ALN  ++ +  + +   
Sbjct: 243 SNEEALANFAKWEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIK 302

Query: 309 KEQLQFGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHLKAAKSSIDDLKESF 368
           K+        E   + +SSE GK++K ++ ++K MT  S S   H+  ++S+   L    
Sbjct: 303 KK-------IEEPLRRMSSESGKSMKEVSISLKNMT-ISSSFDIHVVNSQSACKTLSTLL 362

Query: 369 ----------FKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKSVEPNVSVSSNKP 428
                      + ++  T   +L  +V   E +SE+V EL+  A FK        +  KP
Sbjct: 363 KSGILNDVEPLQMISLMTTVSLLIDIVNLTEKISESVHELASAAKFK--------NKKKP 422

Query: 429 LLLHSGIVNPLF-EETEDEQPHVFDLL 445
              +SG +      ++ D+  HV  +L
Sbjct: 423 SKSNSGSIGQAMPNKSHDDDDHVVTIL 431

BLAST of Lsi09G007420 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 307.4 bits (786), Expect = 2.3e-83
Identity = 183/440 (41.59%), Postives = 266/440 (60.45%), Query Frame = 0

Query: 19  QLGQDDPRIIIHSIKVGMALTLVSLFYYLR---PLYDDFGVSGISAVITVVVIFEFTVGA 78
           ++G +DPR IIH+ KVG+AL LVS FYY +   P  D FG++ + AV+TVVV+FEF+VGA
Sbjct: 13  RVGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGA 72

Query: 79  TLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLRFLPVIKA 138
           TL KG NRG+   +A  L +G + LA+LSG   EP +L +LVF+   L+TF+RF P +K 
Sbjct: 73  TLGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKT 132

Query: 139 RYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCPVWAGETL 198
           ++DYG+L+FI+TF ++S+SG+       LA  RL  V+ GG  CI+ISI VCPVWAG+ L
Sbjct: 133 KFDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDL 192

Query: 199 HDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYKVVLDSNTTEESLE 258
           H  + SN D L+++L+ FG+EYF   E  +  V  +EK K  L+ YK VLDS + EE+L 
Sbjct: 193 HSLLASNFDTLSHFLQDFGDEYFEAREKGDYKV--VEKRKKNLERYKSVLDSKSDEEALA 252

Query: 259 NFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGIEALNGYLSPENNLYISQSKEQLQFG 318
           N+A WEP HG+F F HPWKQY+ +G+  RQCAY I+ALN Y++ +  + +   K+     
Sbjct: 253 NYAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKK----- 312

Query: 319 RTTEVLCKNLSSELGKTLKALATAMKTMTDPSPSSQFHL---KAAKSSIDDLKESF---- 378
              E   + +SSE G ++K ++ ++K M   S SS  H+   +AA  S+  L +S     
Sbjct: 313 --LETPLRRMSSESGNSMKEMSISLKQMI-KSSSSDIHVSNSQAACKSLSTLLKSGILND 372

Query: 379 ---FKSVATATVAPILYAMVKSVEGLSEAVAELSLKAHFKS-VEPNVSVSSNKPLLLHSG 438
               + ++  T   +L  +V   E +SE+V EL+  A FK+ + P V    +      SG
Sbjct: 373 VEPLQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLYEKS-----DSG 432

Query: 439 IVNPLFEETEDEQPHVFDLL 445
            +         E  HV  +L
Sbjct: 433 SIGRAMPIDSHEDHHVVTVL 437

BLAST of Lsi09G007420 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 305.4 bits (781), Expect = 8.8e-83
Identity = 176/434 (40.55%), Postives = 256/434 (58.99%), Query Frame = 0

Query: 9   KLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAVITVVVI 68
           K+++   + +++ ++DPR ++HS KVG+ L LVS FYY +PLYD FGV+ + AV+TVVV+
Sbjct: 3   KVREIVREGRRVAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVV 62

Query: 69  FEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDVLATFLR 128
           FEF+VGATL KG NR      A  L +G ++LA +SG  GEP +L + VF+   L+TF+R
Sbjct: 63  FEFSVGATLGKGLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVR 122

Query: 129 FLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIIISIVVCP 188
           F P +KARYDY +L+FI+TF ++SVSG+       L ++R+  V+ GG  C+IISI VCP
Sbjct: 123 FFPRVKARYDYSLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCP 182

Query: 189 VWAGETLHDSVTSNIDKLANYLEG--------------------------FGEEYFHCSE 248
           VWAG+ LH  + SN +KL+ +L G                          FG++Y  C  
Sbjct: 183 VWAGQDLHSLIASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKY--CEV 242

Query: 249 DEEEHVTDIEKDKSFLQEYKVVLDSNTTEESLENFARWEPKHGKFGFHHPWKQYLKIGSF 308
            E +   +++K K     YK VL+S + EESL NFA+WEP HG+F F HPWKQYL +G  
Sbjct: 243 VENDGAKEVDKRKKDFDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGEL 302

Query: 309 ARQCAYGIEALNGYLSPENNLYISQSKEQLQFGRTTEVLCKNLSSELGKTLKALATAMKT 368
            RQCAY I ALN YL+ +N + +   K      +  E L + +S E GK +K ++ ++K 
Sbjct: 303 IRQCAYRIHALNSYLNADNQVSVDIKK------KLGEPL-RRMSLESGKAMKEMSISLKK 362

Query: 369 MTDPSPSSQFHLKAAKSSIDDLKESF----------FKSVATATVAPILYAMVKSVEGLS 407
           MT PS SS  H++ AKS+   L               + V+  T   +L  ++   E + 
Sbjct: 363 MTKPS-SSDLHVQNAKSACKSLTNLLNSGILKEVEPLELVSLLTAISLLIDIINLTEKIL 422

BLAST of Lsi09G007420 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 260.8 bits (665), Expect = 2.5e-69
Identity = 163/391 (41.69%), Postives = 232/391 (59.34%), Query Frame = 0

Query: 3   IQALAAKLKKKAMKIKQLGQDDPRIIIHSIKVGMALTLVSLFYYLRPLYDDFGVSGISAV 62
           I    AK  +KA +I   G DDP  ++H +KVG+AL+LVS+FYY+RPLYD  G + + A+
Sbjct: 45  IMERVAKFMRKAWRI---GADDPAKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAI 104

Query: 63  ITVVVIFEFTVGATLCKGFNRGLGIFLASALAVGVNYLAKLSGHKGEPFVLGILVFLIDV 122
           +TVVV+FE  VGAT CK  NR +   LA +L + V+++A  SG K E FV+G  VFL   
Sbjct: 105 MTVVVVFESNVGATFCKCVNRVVATILAGSLGIAVHWVATQSG-KAEVFVIGCSVFLFAF 164

Query: 123 LATFLRFLPVIKARYDYGVLMFIVTFTIVSVSGYWINNFFTLAYRRLFAVLAGGAICIII 182
            AT+ RF+P  KAR+DYG ++FI+TF++VSV GY ++    LA +R+  +  G +ICIII
Sbjct: 165 AATYSRFVPSFKARFDYGAMIFILTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIII 224

Query: 183 SIVVCPVWAGETLHDSVTSNIDKLANYLEGFGEEYFHCSEDEEEHVTDIEKDKSFLQEYK 242
           ++  CP+WAG  LH  +  N++KLA+ L+G   EYF  +E       D E     LQ +K
Sbjct: 225 TVFFCPIWAGSQLHRLIERNLEKLADSLDGCVAEYFKENEVSTNRNED-ENTNMKLQGFK 284

Query: 243 VVLDSNTTEESL------------ENFARWEPKHGKFGFHHPWKQYLKIGSFARQCAYGI 302
            VL+S  TEE++             N ARWEP HG F F HPWK Y+KIG+  R+CAY +
Sbjct: 285 CVLNSKGTEEAMPLIRFSGFSFSQANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCL 344

Query: 303 EALNGYLSPENNLYISQSKEQLQ--FGRTTEVLCKNLSSELGKTLKALATAMKTMTDPSP 362
           E L+  ++     Y +++ +Q++  FG      C  LSS   K L+ LA  MK  T  S 
Sbjct: 345 ENLSICIN-----YETEAPDQVKNHFGEA----CMKLSSASSKILRELADMMKN-TRKSS 404

Query: 363 SSQFHLKAAKSSIDDLKESFFKSVATATVAP 380
              F +    S++ +L+E+  K+V   T  P
Sbjct: 405 KMDFLVFDMNSAVQELQET-LKNVPIETNKP 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRM92.2e-9945.45Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q9SJE81.0e-8840.94Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9SJE93.3e-8241.59Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q9XIN11.2e-8140.55Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Q76LB17.0e-7742.36Aluminum-activated malate transporter 1 OS=Triticum aestivum OX=4565 GN=ALMT1 PE... [more]
Match NameE-valueIdentityDescription
A0A5D3DNX68.2e-15367.83Aluminum-activated malate transporter 8-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3B7M18.2e-15367.83aluminum-activated malate transporter 8-like OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1J4971.9e-14966.44aluminum-activated malate transporter 8-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FAA44.2e-14965.56aluminum-activated malate transporter 8-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DEM99.3e-14965.36aluminum-activated malate transporter 8-like OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
XP_008442919.11.7e-15267.83PREDICTED: aluminum-activated malate transporter 8-like [Cucumis melo] >KAA00438... [more]
XP_031737867.12.7e-15066.59aluminum-activated malate transporter 8 [Cucumis sativus] >KAE8651115.1 hypothet... [more]
XP_023526244.16.0e-15066.44aluminum-activated malate transporter 8-like [Cucurbita pepo subsp. pepo][more]
KAG6580748.11.7e-14966.22Aluminum-activated malate transporter 8, partial [Cucurbita argyrosperma subsp. ... [more]
KAG7017502.12.3e-14964.73Aluminum-activated malate transporter 8, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
AT3G11680.11.6e-10045.45Aluminium activated malate transporter family protein [more]
AT1G08440.17.4e-9040.94Aluminium activated malate transporter family protein [more]
AT1G08430.12.3e-8341.59aluminum-activated malate transporter 1 [more]
AT2G27240.18.8e-8340.55Aluminium activated malate transporter family protein [more]
AT4G00910.12.5e-6941.69Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 19..368
e-value: 2.1E-130
score: 435.7
NoneNo IPR availablePANTHERPTHR31086:SF22ALUMINUM-ACTIVATED MALATE TRANSPORTER 8coord: 4..413
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 4..413

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G007420.1Lsi09G007420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015743 malate transport
cellular_component GO:0009705 plant-type vacuole membrane