Lsi09G006360 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G006360
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAcyl-[acyl-carrier-protein] hydrolase
Locationchr09: 7043308 .. 7046485 (-)
RNA-Seq ExpressionLsi09G006360
SyntenyLsi09G006360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAAAGCGTCCAAATTTCAAACTACCCATTTGTCCTTCAACACTCCATTGTCGATCAAAACTAAGATTCCATAGCTACTTCAAAGAAAAATGTCAAATCCTTCCCATTTTCCGATGGAAATACCCATTTCTTTGTTATGTTCATCTCTCAACTGAGAAAAAAAGAAATTGGTATCTTCTTCCTTAGGCAAATCCGCCATTTGCAACTATAAGATCGTGATGGCTGTTCCTCTGTTTCAGAAGCTCGACCAATGGCCTCTTTCTCTTGCCCTCTTCCTTATGTTGTTAGATGCTCCACTGCCAAAGAAGGTAGTGATTCTAACAAGCAGAGGTATGTTAAAGTGAATGGGGCTATTATTGGGTCCATTTCTGAAGCTGATTTGTTGAGCCAATCGGTTAGAGCTTCCAGGGCTCTCATCCCTTCTGTGGCGGAAAATGGGTGTTTTGTTAAAGAAGATGTTCGTCAGAAAATTCCCACGAAGAAGCAGCTGGTTGATCCTCATCGGCAAGGCCTGATCATTGAAGGAGGTGTTGGGTATAGACAAACTGTTGTAATCAGATCGTATGAAGTTGGTCCTGATAAGACTGCAACTGTGGAGAGCGTAATGAATCTTCTGCAGGTCAGGGATGCCAAAATTATGTTCGAAGAAATGCCTGAGAGAAGTTTTTGAATTGGGCACTCGCTCTAATTGGTTGGTACCATTTGTGATTCATGTAGGAAACAGCACTTAATCATGTTTGGATGTCTGGGCTTCTCAGCAATGGTTTTGGGGCCACCCATGGCATGATGAGGAACAATCTTATCTGGGTCGTTTCGCGAATGCTCGTCGAAGTAGACCACTATCCGATATGGTATGTTGAATGTTCTCTGGTAATCATCAAAAGAGTAATCTTACAGAACTTCAAGTTTTTAGTGTTTTTGTCTAAACACAATTGGATATTCATCTAGATAGTTGTGGATTCATTTTAACAGTCAGCTTTCTGGTTTTTTTCTTGAACAATGAGTTACTGTCCTGCCTGATGTCTTGTCCCAAAAGAGAAAACATGCCTACTAATACAGAACACTGCATTATATATTCTATAGGTTAGGTTGATATATACTTGAATAGAGCTTCCTAGAGGTTAGAAAACTAACTAACTCATCAGTTTAAATCTGAGTCTTGGGATGATAGTCTTCGTACTTTGGTCATGGGGATATCAATTTCAAATAAAGAACTTGTTATTTACTTGTACTTGAAATGTTACTCATGTATTATGCATATGGTGGATCATTCTGCAGGGGTGAGATTGTTGAAATCGATACATGGGTAGGGGCGTCGGGTAAAAATGGAATGAGAAGAGACTGGCTAATTAGAAGTCAAGCCACAGGTCATGTTTATGCTAGAGGAACAAGGTAATAAGAACTTACATGACATGTCTTCCAAATTTGTACCCGGTTGCTGTCGTCTTTCCTGGTGTACTAAAATGGTGAAACATAAAACAGCACGTGGGTGATGATGAACCAGCATACAAGACGACTCTCCAAAATGCCAGAAGAGGTAAGGGCAGAGATTTCACCATGGTTTATAGAAAAGCATGCAATCAAAGAAGATGCCCCCGAGAAAATATCCAAGTTAGATAACAATGCTAAGTACGTGAACTCTGACTTGAAGGTAAAATCCATTTTAACTCAACTACAATTCAGTCTCCATCAGCATTTAGAAATTTTTTTTTTGCTGATAATTGCTATTTACCCAGCCAAAGAGAAGTGATCTGGACATGAACCACCATGTCAACAATGTCAAATACCTAAGATGGATGCTAGAGGTATTGTATATACAAAATAATCATTTATCAATAGATTGGGGGGGGGGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAGGGGGGGGGGGGGGGGGGGGGGTGTCCAAGTAAGTTTTGACACTTGATCATTTGCCTATTACAGACGATACCCGACCATGTTTTGGAGTCTCATCAACTTTCTAGTATTGTGTTAGAGTATAGAAGGGAATGCGGAAGCTCTGATATAGTTCAATCACTATGTGAGCCAGAGGAAGATGGAATCCTTAGAGATGTGGACATGATAGAAGACAGTGAAAGTCTACTAAATGGGTTTTCGTTGGCATCTGAGATTCTCAAAGGTGGTGGGCTCTTGGGATCTTTTGACAAAGGAAAACTGAGGTATACACATCTTCTACAAATTGAAGGGGAAGCTAAGAATGAAGAGATAGTGAGGGGAAGAACCACATGGAAGAAAAAGCTTACCACTATACCATTTTCCACACAGTAAATCTCATTTGTAGCAGTTTCATGCCTCTTTATGAAGAAAGAAGACAACACCCACAATGCAACATTTCTGAAAACAATAAGCAAGGGCTGCTGCTTTATGTGAAATTTATTATTGTTAATGATTTTTCATATTCATAGTCTTGTTGTTCTGCTTCACAAGGGAGTTAATCAGATGGAGCTGCTCCTGCAAATGCAGAAGTAA

mRNA sequence

CCAAAAGCGTCCAAATTTCAAACTACCCATTTGTCCTTCAACACTCCATTGTCGATCAAAACTAAGATTCCATAGCTACTTCAAAGAAAAATGTCAAATCCTTCCCATTTTCCGATGGAAATACCCATTTCTTTGTTATGTTCATCTCTCAACTGAGAAAAAAAGAAATTGGTATCTTCTTCCTTAGGCAAATCCGCCATTTGCAACTATAAGATCGTGATGGCTGTTCCTCTGTTTCAGAAGCTCGACCAATGGCCTCTTTCTCTTGCCCTCTTCCTTATGTTGTTAGATGCTCCACTGCCAAAGAAGGTAGTGATTCTAACAAGCAGAGGTATGTTAAAGTGAATGGGGCTATTATTGGGTCCATTTCTGAAGCTGATTTGTTGAGCCAATCGGTTAGAGCTTCCAGGGCTCTCATCCCTTCTGTGGCGGAAAATGGGTGTTTTGTTAAAGAAGATGTTCGTCAGAAAATTCCCACGAAGAAGCAGCTGGTTGATCCTCATCGGCAAGGCCTGATCATTGAAGGAGGTGTTGGGTATAGACAAACTGTTGTAATCAGATCGTATGAAGTTGGTCCTGATAAGACTGCAACTGTGGAGAGCGTAATGAATCTTCTGCAGGAAACAGCACTTAATCATGTTTGGATGTCTGGGCTTCTCAGCAATGGTTTTGGGGCCACCCATGGCATGATGAGGAACAATCTTATCTGGGTCGTTTCGCGAATGCTCGTCGAAGTAGACCACTATCCGATATGGGGTGAGATTGTTGAAATCGATACATGGGTAGGGGCGTCGGGTAAAAATGGAATGAGAAGAGACTGGCTAATTAGAAGTCAAGCCACAGGTCATGTTTATGCTAGAGGAACAAGCACGTGGGTGATGATGAACCAGCATACAAGACGACTCTCCAAAATGCCAGAAGAGGTAAGGGCAGAGATTTCACCATGGTTTATAGAAAAGCATGCAATCAAAGAAGATGCCCCCGAGAAAATATCCAAGTTAGATAACAATGCTAAGTACGTGAACTCTGACTTGAAGACGATACCCGACCATGTTTTGGAGTCTCATCAACTTTCTAGTATTGTGTTAGAGTATAGAAGGGAATGCGGAAGCTCTGATATAGTTCAATCACTATGTGAGCCAGAGGAAGATGGAATCCTTAGAGATGTGGACATGATAGAAGACAGTGAAAGTCTACTAAATGGGTTTTCGTTGGCATCTGAGATTCTCAAAGGTGGTGGGCTCTTGGGATCTTTTGACAAAGGAAAACTGAGTCTTGTTGTTCTGCTTCACAAGGGAGTTAATCAGATGGAGCTGCTCCTGCAAATGCAGAAGTAA

Coding sequence (CDS)

ATGGCCTCTTTCTCTTGCCCTCTTCCTTATGTTGTTAGATGCTCCACTGCCAAAGAAGGTAGTGATTCTAACAAGCAGAGGTATGTTAAAGTGAATGGGGCTATTATTGGGTCCATTTCTGAAGCTGATTTGTTGAGCCAATCGGTTAGAGCTTCCAGGGCTCTCATCCCTTCTGTGGCGGAAAATGGGTGTTTTGTTAAAGAAGATGTTCGTCAGAAAATTCCCACGAAGAAGCAGCTGGTTGATCCTCATCGGCAAGGCCTGATCATTGAAGGAGGTGTTGGGTATAGACAAACTGTTGTAATCAGATCGTATGAAGTTGGTCCTGATAAGACTGCAACTGTGGAGAGCGTAATGAATCTTCTGCAGGAAACAGCACTTAATCATGTTTGGATGTCTGGGCTTCTCAGCAATGGTTTTGGGGCCACCCATGGCATGATGAGGAACAATCTTATCTGGGTCGTTTCGCGAATGCTCGTCGAAGTAGACCACTATCCGATATGGGGTGAGATTGTTGAAATCGATACATGGGTAGGGGCGTCGGGTAAAAATGGAATGAGAAGAGACTGGCTAATTAGAAGTCAAGCCACAGGTCATGTTTATGCTAGAGGAACAAGCACGTGGGTGATGATGAACCAGCATACAAGACGACTCTCCAAAATGCCAGAAGAGGTAAGGGCAGAGATTTCACCATGGTTTATAGAAAAGCATGCAATCAAAGAAGATGCCCCCGAGAAAATATCCAAGTTAGATAACAATGCTAAGTACGTGAACTCTGACTTGAAGACGATACCCGACCATGTTTTGGAGTCTCATCAACTTTCTAGTATTGTGTTAGAGTATAGAAGGGAATGCGGAAGCTCTGATATAGTTCAATCACTATGTGAGCCAGAGGAAGATGGAATCCTTAGAGATGTGGACATGATAGAAGACAGTGAAAGTCTACTAAATGGGTTTTCGTTGGCATCTGAGATTCTCAAAGGTGGTGGGCTCTTGGGATCTTTTGACAAAGGAAAACTGAGTCTTGTTGTTCTGCTTCACAAGGGAGTTAATCAGATGGAGCTGCTCCTGCAAATGCAGAAGTAA

Protein sequence

MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVAENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPEKISKLDNNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSESLLNGFSLASEILKGGGLLGSFDKGKLSLVVLLHKGVNQMELLLQMQK
Homology
BLAST of Lsi09G006360 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.0e-63
Identity = 124/273 (45.42%), Postives = 171/273 (62.64%), Query Frame = 0

Query: 80  LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNG 139
           ++DP   G I++ G+ + Q   IRSYE+G D+TA++E++MN LQETA+NH   +GLL  G
Sbjct: 118 VIDPFGIGKIVQDGLVFSQNFSIRSYEIGADQTASIETLMNHLQETAINHCRSAGLLGEG 177

Query: 140 FGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGH 199
           FGAT  M + NLIWVV+RM V VD YP WG++V++DTWV ASGKNGMRRDWL+ +  TG 
Sbjct: 178 FGATPEMCKKNLIWVVTRMQVVVDRYPTWGDVVQVDTWVSASGKNGMRRDWLVSNSETGE 237

Query: 200 VYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPEKISKLD-------- 259
           +  R TS WVMMN+ TRRLSK+PEEVR EI P+F+    +  +  +K+ KLD        
Sbjct: 238 ILTRATSVWVMMNKLTRRLSKIPEEVRGEIEPFFMNSDPVLAEDSQKLVKLDDSTAEHVC 297

Query: 260 ----------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQSLC 319
                           NN KY+   L++ P  +LESH+LS++ LEYRRECG   ++QSL 
Sbjct: 298 KGLTPKWSDLDVNQHVNNVKYIGWILESAPLPILESHELSALTLEYRRECGRDSVLQSLT 357

Query: 320 EPEEDGILRDVDMIEDSESLLNGFSLASEILKG 329
              +      V++ E +   L      +EI++G
Sbjct: 358 TVSDSNTENAVNVGEFNCQHLLRLDDGAEIVRG 390

BLAST of Lsi09G006360 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 7.3e-62
Identity = 118/239 (49.37%), Postives = 156/239 (65.27%), Query Frame = 0

Query: 80  LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNG 139
           LVDP   G I++ G+ +RQ   IRSYE+G D++A++E+VMN LQETALNHV  +GLL +G
Sbjct: 123 LVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVKTAGLLGDG 182

Query: 140 FGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGH 199
           FG+T  M + NLIWVV+RM V VD YP WG++VE+DTWV  SGKNGMRRDWL+R   TG 
Sbjct: 183 FGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWLVRDCNTGE 242

Query: 200 VYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPEKISKLD-------- 259
              R +S WVMMN+ TRRLSK+PEEVR EI P+F+    +  +   K++K+D        
Sbjct: 243 TLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAEDSRKLTKIDDKTADYVR 302

Query: 260 ----------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQSL 295
                           NN KY+   L++ P  ++E  +L S+ LEYRRECG   ++QSL
Sbjct: 303 SGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSL 361

BLAST of Lsi09G006360 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.2e-61
Identity = 119/246 (48.37%), Postives = 158/246 (64.23%), Query Frame = 0

Query: 80  LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNG 139
           LVDP   G I++ G+ +RQ   IRSYE+G D+TA++E+VMN LQETALNHV ++GL ++G
Sbjct: 122 LVDPFGLGSIVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKIAGLSNDG 181

Query: 140 FGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGH 199
           FG T  M + +LIWVV++M V V+ YP WG+ VE++TWV  SGKNGMRRDWLI    TG 
Sbjct: 182 FGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGE 241

Query: 200 VYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPEKISKLD-------- 259
           +  R +S WVMMNQ TRRLSK+P+EVR EI P F++   + ED   K+ KLD        
Sbjct: 242 ILTRASSVWVMMNQKTRRLSKIPDEVRNEIEPHFVDSPPVIEDDDRKLPKLDEKTADSIR 301

Query: 260 ----------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQSLC 302
                           NN KY+   L++ P  VLE+ +L S+ LEYRRECG   +++SL 
Sbjct: 302 KGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLT 361

BLAST of Lsi09G006360 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 4.0e-60
Identity = 117/246 (47.56%), Postives = 157/246 (63.82%), Query Frame = 0

Query: 80  LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNG 139
           L DP   G +++ G+ +RQ   IRSYE+G D+TA++E+VMN LQETALNHV  +GL ++G
Sbjct: 119 LEDPFGLGRVVQDGLVFRQNFSIRSYEIGADRTASIETVMNHLQETALNHVKTAGLSNDG 178

Query: 140 FGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGH 199
           FG T  M + +LIWVV++M V V+ YP WG+ VE++TWV  SGKNGMRRDWLI    TG 
Sbjct: 179 FGRTPEMYKRDLIWVVAKMQVMVNRYPTWGDTVEVNTWVAKSGKNGMRRDWLISDCNTGE 238

Query: 200 VYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPEKISKLD-------- 259
           +  R +S WVMMNQ TR+LSK+P+EVR EI P F++   + ED   K+ KLD        
Sbjct: 239 ILTRASSVWVMMNQKTRKLSKIPDEVRREIEPHFVDSAPVIEDDDRKLPKLDEKSADSIR 298

Query: 260 ----------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQSLC 302
                           NNAKY+   L++ P  VLE+ +L S+ LEYRRECG   +++SL 
Sbjct: 299 KGLTPRWNDLDVNQHVNNAKYIGWILESTPPEVLETQELCSLTLEYRRECGRESVLESLT 358

BLAST of Lsi09G006360 vs. ExPASy Swiss-Prot
Match: G3ESV1 (Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissima OX=857185 GN=FATB3 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.9e-55
Identity = 132/366 (36.07%), Postives = 192/366 (52.46%), Query Frame = 0

Query: 23  SNKQRYVKVNGAII----GSISEADLLSQSVRASRALIPSVAENGCFVKEDVRQKIPTKK 82
           +N   + K NG+ +    GS+   +  S      R  I  + +    V       +  +K
Sbjct: 50  ANASAHPKANGSAVSLKAGSLETQEDTSSPSPPPRTFISQLPDWSMLVSAITTVFVAAEK 109

Query: 83  Q-------------LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQET 142
           Q             LV+P      ++ GV +RQ+  IRSYE+G D+TA++E++MN+ QET
Sbjct: 110 QWTMLDRKSKRPDVLVEP-----FVQDGVSFRQSFSIRSYEIGVDRTASIETLMNIFQET 169

Query: 143 ALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNG 202
           +LNH    GLL++GFG T  M + +LIWVV++M +EV+ YP WG+ +E+ TWV  SGKNG
Sbjct: 170 SLNHCKSLGLLNDGFGRTPEMCKRDLIWVVTKMQIEVNRYPTWGDTIEVTTWVSESGKNG 229

Query: 203 MRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPE 262
           M RDWLI    +G +  R TS W MMNQ TRRLSK+P+EVR EI P+F++   + ED   
Sbjct: 230 MSRDWLISDCHSGEILIRATSVWAMMNQKTRRLSKIPDEVRQEIVPYFVDSAPVIED-DR 289

Query: 263 KISKLD------------------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEY 322
           K+ KLD                        NN KY+   LK++P  V E+ +L  + LEY
Sbjct: 290 KLHKLDVKTGDSIRNGLTPRWNDFDVNQHVNNVKYIAWLLKSVPTEVFETQELCGLTLEY 349

Query: 323 RRECGSSDIVQSL--CEPEEDGILRDVDMIEDSESLLNGFSLASEIL----KGGGLLGSF 342
           RREC    +++S+   +P ++G   D  + +    L NG  +A        K  G  G+ 
Sbjct: 350 RRECRRDSVLESVTAMDPSKEG---DRSLYQHLLRLENGADIALGRTEWRPKNAGATGAV 406

BLAST of Lsi09G006360 vs. ExPASy TrEMBL
Match: A0A5D3E585 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00050 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.1e-177
Identity = 326/370 (88.11%), Postives = 335/370 (90.54%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYV+RCSTAKEG+DSNK RYVKVNGAIIGSISEADLLSQSVRASRAL+PSVA
Sbjct: 1   MASFSCPVPYVIRCSTAKEGNDSNKLRYVKVNGAIIGSISEADLLSQSVRASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYAR TSTWVMMNQ TRRLSKMPEEVRAEISPWFIEK AIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARATSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKQAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKLD+ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIVQSLCEP+EDGILRDV+MIEDSE SLLNGFSLASEILK GGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPDEDGILRDVEMIEDSEISLLNGFSLASEILK-GGLLGSFD 360

BLAST of Lsi09G006360 vs. ExPASy TrEMBL
Match: A0A1S3CSW2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC103504583 PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.2e-176
Identity = 324/370 (87.57%), Postives = 334/370 (90.27%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYV+RCSTAKEG+DSNK RY KVNGAIIGSISEADLLSQSVRASRAL+PSVA
Sbjct: 1   MASFSCPVPYVIRCSTAKEGNDSNKLRYAKVNGAIIGSISEADLLSQSVRASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYAR TSTWVMMNQ TRRLSKMPEEVRAEISPWFIEK AIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARATSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKQAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKL++ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLNDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIVQSLCEP+EDGILRDV+MIEDSE SLLNGFSLASEILK GGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPDEDGILRDVEMIEDSEISLLNGFSLASEILK-GGLLGSFD 360

BLAST of Lsi09G006360 vs. ExPASy TrEMBL
Match: A0A0A0KT05 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G576640 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 5.1e-175
Identity = 319/362 (88.12%), Postives = 329/362 (90.88%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYV+RCSTAKEG+DSNK RYVKVNGAIIGSISE DLLSQSVRASRAL+PSVA
Sbjct: 1   MASFSCPVPYVIRCSTAKEGNDSNKLRYVKVNGAIIGSISETDLLSQSVRASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATG+VYAR TSTWVMMNQ TRRLSKMPEEVRAEISPWFIEK AIK
Sbjct: 181 SGKNGMRRDWLIRSQATGYVYARATSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKQAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKLD+ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 339
           +LEYRRECGSSDIVQSLCEP+EDGILRDV+MIED E  LLNGFSLASEILK GGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPDEDGILRDVEMIEDREIGLLNGFSLASEILK-GGLLGSFD 360

BLAST of Lsi09G006360 vs. ExPASy TrEMBL
Match: A0A6J1FHN8 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111445440 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.3e-170
Identity = 314/370 (84.86%), Postives = 327/370 (88.38%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYVVRCS AKEG D N+QRY KVNG II SISEADLLS+S +ASRALIPSVA
Sbjct: 1   MASFSCPVPYVVRCSIAKEGGDFNRQRYAKVNGGIIRSISEADLLSRSAKASRALIPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENG  VKEDVRQKIPTKKQLVDP+RQGLIIEGGVGYRQTVVIRSYEVGPDKTAT+ES+MN
Sbjct: 61  ENGFVVKEDVRQKIPTKKQLVDPYRQGLIIEGGVGYRQTVVIRSYEVGPDKTATMESLMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMM++NLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMKHNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQ TRRLSKMPEEVRAEISPWFIEKHAIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKHAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDA EKISKLD+ AKY+NSDLK                       TIPDHVLES QLSSI
Sbjct: 241 EDAYEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESQQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIVQSLCEPEE+GILRDVDMIEDSE SLLNGFSLASEILKGGGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPEENGILRDVDMIEDSEISLLNGFSLASEILKGGGLLGSFD 360

BLAST of Lsi09G006360 vs. ExPASy TrEMBL
Match: A0A6J1JSD6 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111489345 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.9e-170
Identity = 314/370 (84.86%), Postives = 326/370 (88.11%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYVVRCS AKEG D NKQRY KVNG II SISEADLLS+S +ASRALIPSVA
Sbjct: 1   MASFSCPVPYVVRCSIAKEGGDFNKQRYAKVNGGIIRSISEADLLSRSAKASRALIPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENG  VKEDVRQKIPTKKQLVDP+RQGLIIEGGVGYRQTVVIRSYEVGPDKTAT+ES+MN
Sbjct: 61  ENGFVVKEDVRQKIPTKKQLVDPYRQGLIIEGGVGYRQTVVIRSYEVGPDKTATMESLMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMM++NLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMKHNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQ TRRLSKMPEEVRAEISPWFIEKHAIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKHAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDA EKISKLD+ AKY+NSDLK                       TIPDHVLES QLSSI
Sbjct: 241 EDAYEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESQQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIV+SLCEPEE GILRDVDMIEDSE SLLNGFSLASEILKGGGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVKSLCEPEEKGILRDVDMIEDSEISLLNGFSLASEILKGGGLLGSFD 360

BLAST of Lsi09G006360 vs. NCBI nr
Match: XP_038907219.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 646.4 bits (1666), Expect = 1.5e-181
Identity = 328/369 (88.89%), Postives = 336/369 (91.06%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PY+VRCSTAKEG+DSNKQRYVK+NGAIIGSISE DLLSQSVRASRALIPSVA
Sbjct: 1   MASFSCPVPYIVRCSTAKEGNDSNKQRYVKLNGAIIGSISEPDLLSQSVRASRALIPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTAT+ESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATLESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQ TRRLSKMPEEVRAEISPWFIEKHAIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKHAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKLD+ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSESLLNGFSLASEILKGGGLLGSFDK 347
           VLEYRRECGSSD+VQSLCE EEDGILRDVDMIED ESLLNGFSLASEILKGGGLLGSFDK
Sbjct: 301 VLEYRRECGSSDVVQSLCEAEEDGILRDVDMIEDGESLLNGFSLASEILKGGGLLGSFDK 360

BLAST of Lsi09G006360 vs. NCBI nr
Match: KAA0046300.1 (palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK30465.1 palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa])

HSP 1 Score: 632.5 bits (1630), Expect = 2.3e-177
Identity = 326/370 (88.11%), Postives = 335/370 (90.54%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYV+RCSTAKEG+DSNK RYVKVNGAIIGSISEADLLSQSVRASRAL+PSVA
Sbjct: 1   MASFSCPVPYVIRCSTAKEGNDSNKLRYVKVNGAIIGSISEADLLSQSVRASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYAR TSTWVMMNQ TRRLSKMPEEVRAEISPWFIEK AIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARATSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKQAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKLD+ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIVQSLCEP+EDGILRDV+MIEDSE SLLNGFSLASEILK GGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPDEDGILRDVEMIEDSEISLLNGFSLASEILK-GGLLGSFD 360

BLAST of Lsi09G006360 vs. NCBI nr
Match: XP_008467164.1 (PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 629.0 bits (1621), Expect = 2.5e-176
Identity = 324/370 (87.57%), Postives = 334/370 (90.27%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYV+RCSTAKEG+DSNK RY KVNGAIIGSISEADLLSQSVRASRAL+PSVA
Sbjct: 1   MASFSCPVPYVIRCSTAKEGNDSNKLRYAKVNGAIIGSISEADLLSQSVRASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYAR TSTWVMMNQ TRRLSKMPEEVRAEISPWFIEK AIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARATSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKQAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKL++ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLNDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIVQSLCEP+EDGILRDV+MIEDSE SLLNGFSLASEILK GGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPDEDGILRDVEMIEDSEISLLNGFSLASEILK-GGLLGSFD 360

BLAST of Lsi09G006360 vs. NCBI nr
Match: XP_011655469.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 623.6 bits (1607), Expect = 1.0e-174
Identity = 319/362 (88.12%), Postives = 329/362 (90.88%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYV+RCSTAKEG+DSNK RYVKVNGAIIGSISE DLLSQSVRASRAL+PSVA
Sbjct: 1   MASFSCPVPYVIRCSTAKEGNDSNKLRYVKVNGAIIGSISETDLLSQSVRASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN
Sbjct: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATG+VYAR TSTWVMMNQ TRRLSKMPEEVRAEISPWFIEK AIK
Sbjct: 181 SGKNGMRRDWLIRSQATGYVYARATSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKQAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDAPEKISKLD+ AKY+NSDLK                       TIPDHVLESHQLSSI
Sbjct: 241 EDAPEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESHQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 339
           +LEYRRECGSSDIVQSLCEP+EDGILRDV+MIED E  LLNGFSLASEILK GGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPDEDGILRDVEMIEDREIGLLNGFSLASEILK-GGLLGSFD 360

BLAST of Lsi09G006360 vs. NCBI nr
Match: KAG6579230.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 609.8 bits (1571), Expect = 1.6e-170
Identity = 314/370 (84.86%), Postives = 327/370 (88.38%), Query Frame = 0

Query: 1   MASFSCPLPYVVRCSTAKEGSDSNKQRYVKVNGAIIGSISEADLLSQSVRASRALIPSVA 60
           MASFSCP+PYVVRCS AKEG D NKQRY KVNG II SISEADLLS+S +ASRAL+PSVA
Sbjct: 1   MASFSCPVPYVVRCSIAKEGGDFNKQRYAKVNGGIIRSISEADLLSRSAKASRALVPSVA 60

Query: 61  ENGCFVKEDVRQKIPTKKQLVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMN 120
           ENG  VKEDVRQKIPTKKQLVDP+RQGLIIEGGVGYRQTVVIRSYEVGPDKTAT+ES+MN
Sbjct: 61  ENGFVVKEDVRQKIPTKKQLVDPYRQGLIIEGGVGYRQTVVIRSYEVGPDKTATMESLMN 120

Query: 121 LLQETALNHVWMSGLLSNGFGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180
           LLQETALNHVWMSGLLSNGFGATHGMM++NLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA
Sbjct: 121 LLQETALNHVWMSGLLSNGFGATHGMMKHNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGA 180

Query: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIK 240
           SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQ TRRLSKMPEEVRAEISPWFIEKHAIK
Sbjct: 181 SGKNGMRRDWLIRSQATGHVYARGTSTWVMMNQQTRRLSKMPEEVRAEISPWFIEKHAIK 240

Query: 241 EDAPEKISKLDNNAKYVNSDLK-----------------------TIPDHVLESHQLSSI 300
           EDA EKISKLD+ AKY+NSDLK                       TIPDHVLES QLSSI
Sbjct: 241 EDAYEKISKLDDKAKYMNSDLKPKRSDLDMNHHVNNVKYLRWMLETIPDHVLESQQLSSI 300

Query: 301 VLEYRRECGSSDIVQSLCEPEEDGILRDVDMIEDSE-SLLNGFSLASEILKGGGLLGSFD 347
           +LEYRRECGSSDIVQSLCEPEE+GILRDVDMIEDSE SLLNGFSLASEILKGGGLLGSFD
Sbjct: 301 ILEYRRECGSSDIVQSLCEPEENGILRDVDMIEDSEISLLNGFSLASEILKGGGLLGSFD 360

BLAST of Lsi09G006360 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 239.2 bits (609), Expect = 5.2e-63
Identity = 118/239 (49.37%), Postives = 156/239 (65.27%), Query Frame = 0

Query: 80  LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNG 139
           LVDP   G I++ G+ +RQ   IRSYE+G D++A++E+VMN LQETALNHV  +GLL +G
Sbjct: 123 LVDPFGIGRIVQDGLVFRQNFSIRSYEIGADRSASIETVMNHLQETALNHVKTAGLLGDG 182

Query: 140 FGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGH 199
           FG+T  M + NLIWVV+RM V VD YP WG++VE+DTWV  SGKNGMRRDWL+R   TG 
Sbjct: 183 FGSTPEMFKKNLIWVVTRMQVVVDKYPTWGDVVEVDTWVSQSGKNGMRRDWLVRDCNTGE 242

Query: 200 VYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAIKEDAPEKISKLD-------- 259
              R +S WVMMN+ TRRLSK+PEEVR EI P+F+    +  +   K++K+D        
Sbjct: 243 TLTRASSVWVMMNKLTRRLSKIPEEVRGEIEPYFVNSDPVLAEDSRKLTKIDDKTADYVR 302

Query: 260 ----------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQSL 295
                           NN KY+   L++ P  ++E  +L S+ LEYRRECG   ++QSL
Sbjct: 303 SGLTPRWSDLDVNQHVNNVKYIGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSL 361

BLAST of Lsi09G006360 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 188.0 bits (476), Expect = 1.4e-47
Identity = 97/243 (39.92%), Postives = 140/243 (57.61%), Query Frame = 0

Query: 80  LVDPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNG 139
           L D  R G + E G+ Y++  V+RSYEVG +KTATVE++ NLLQE   NH    G  ++G
Sbjct: 68  LADQLRLGSLTEDGLSYKEKFVVRSYEVGSNKTATVETIANLLQEVGCNHAQSVGFSTDG 127

Query: 140 FGATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGH 199
           F  T  M + +LIWV +RM +E+  YP WG++VEI+TW  + G+ G RRDW+++   TG 
Sbjct: 128 FATTTTMRKLHLIWVTARMHIEIYKYPAWGDVVEIETWCQSEGRIGTRRDWILKDSVTGE 187

Query: 200 VYARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAI-----KEDAPEKISKLD--- 259
           V  R TS WVMMNQ TRRL K+ ++VR E   +  ++  +        + +KI KL+   
Sbjct: 188 VTGRATSKWVMMNQDTRRLQKVSDDVRDEYLVFCPQEPRLAFPEENNRSLKKIPKLEDPA 247

Query: 260 --------------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIV 295
                               NN  Y+   L++IP  ++++H+L  I L+YRREC   D+V
Sbjct: 248 QYSMIGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIVDTHELQVITLDYRRECQQDDVV 307

BLAST of Lsi09G006360 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 185.7 bits (470), Expect = 6.8e-47
Identity = 98/245 (40.00%), Postives = 141/245 (57.55%), Query Frame = 0

Query: 82  DPHRQGLIIEGGVGYRQTVVIRSYEVGPDKTATVESVMNLLQETALNHVWMSGLLSNGFG 141
           D  R G ++E G  Y++  ++RSYEVG +KTAT+E++ NLLQE A NHV   G  ++GF 
Sbjct: 69  DRLRFGRLMEDGFSYKEKFIVRSYEVGINKTATIETIANLLQEVACNHVQNVGFSTDGFA 128

Query: 142 ATHGMMRNNLIWVVSRMLVEVDHYPIWGEIVEIDTWVGASGKNGMRRDWLIRSQATGHVY 201
            T  M + +LIWV +RM +E+  YP W ++VEI+TW  + G+ G RRDW+++  ATG V 
Sbjct: 129 TTLTMRKLHLIWVTARMHIEIYKYPAWSDVVEIETWCQSEGRIGTRRDWILKDCATGEVI 188

Query: 202 ARGTSTWVMMNQHTRRLSKMPEEVRAEISPWFIEKHAI-----KEDAPEKISKLD----- 261
            R TS WVMMNQ TRRL ++ +EVR E   +   +  +        + +KI KL+     
Sbjct: 189 GRATSKWVMMNQDTRRLQRVTDEVRDEYLVFCPPEPRLAFPEENNSSLKKIPKLEDPAQY 248

Query: 262 ------------------NNAKYVNSDLKTIPDHVLESHQLSSIVLEYRRECGSSDIVQS 299
                             NN  Y+   L++IP  ++++H+L  I L+YRREC   DIV S
Sbjct: 249 SMLGLKPRRADLDMNQHVNNVTYIGWVLESIPQEIIDTHELKVITLDYRRECQQDDIVDS 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SQI35.0e-6345.42Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum... [more]
Q9SJE27.3e-6249.37Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia... [more]
Q395136.2e-6148.37Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ... [more]
G3ESV04.0e-6047.56Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ... [more]
G3ESV13.9e-5536.07Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissim... [more]
Match NameE-valueIdentityDescription
A0A5D3E5851.1e-17788.11Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CSW21.2e-17687.57Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC103504583 PE... [more]
A0A0A0KT055.1e-17588.12Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G576640... [more]
A0A6J1FHN81.3e-17084.86Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A6J1JSD62.9e-17084.86Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11148934... [more]
Match NameE-valueIdentityDescription
XP_038907219.11.5e-18188.89palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Benincasa hispi... [more]
KAA0046300.12.3e-17788.11palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK30465... [more]
XP_008467164.12.5e-17687.57PREDICTED: palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucu... [more]
XP_011655469.11.0e-17488.12palmitoyl-acyl carrier protein thioesterase, chloroplastic isoform X1 [Cucumis s... [more]
KAG6579230.11.6e-17084.86Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a... [more]
Match NameE-valueIdentityDescription
AT1G08510.15.2e-6349.37fatty acyl-ACP thioesterases B [more]
AT3G25110.11.4e-4739.92fatA acyl-ACP thioesterase [more]
AT4G13050.16.8e-4740.00Acyl-ACP thioesterase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 89..317
e-value: 1.0E-59
score: 203.8
NoneNo IPR availablePANTHERPTHR31727:SF10ACYL-[ACYL-CARRIER-PROTEIN] HYDROLASEcoord: 1..347
NoneNo IPR availableCDDcd005864HBTcoord: 96..212
e-value: 1.02884E-12
score: 61.8533
IPR002864Acyl-ACP thioesterasePFAMPF01643Acyl-ACP_TEcoord: 95..299
e-value: 2.0E-46
score: 158.3
IPR045023Acyl-[acyl-carrier-protein] hydrolase FATA/BPANTHERPTHR31727OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTICcoord: 1..347
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 95..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G006360.1Lsi09G006360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016297 acyl-[acyl-carrier-protein] hydrolase activity
molecular_function GO:0000036 acyl carrier activity
molecular_function GO:0016790 thiolester hydrolase activity