Lsi09G005610 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G005610
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr09: 5924335 .. 5924784 (+)
RNA-Seq Expression	Lsi09G005610
Synteny	Lsi09G005610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCTCTTAACATAGATCTGAGTTCAAATCTCACAACATCGTTCATAATGTACCATCCTCTTAGTGGTGGATGTGTGCGAATGAACAAAAAATATCAATTAGGAATCAGTAGTTGTAAGACTTCCAACCGTTGGAGCCATGAAGAAGACAGTGCTCCTATTAGGTTGGCAGGATCTATATTATGTTTGAAAGCTGTAGGAGATGAACTCTCTCCAATTCTCTCTCAAGACTGCTCGAGCCAACAAAGTGTTTGGAAATATGCCTCAAATGCCAAGCTACAACTAGCCACCATCGATAAACAAGGACAGACCTTATGCTTGCAAAGAGCTTCTCATACCCATCAAATCGTGACTAATAAGTGCATGTGTTTGAATGATTCTCAATGTCAAGAAGATCCACAAAGCCAATGGTTTACTCTTGTTCCATCAAATGTACGTCTCATTTAA

mRNA sequence

ATGGTTGCTCTTAACATAGATCTGAGTTCAAATCTCACAACATCGTTCATAATGTACCATCCTCTTAGTGGTGGATGTGTGCGAATGAACAAAAAATATCAATTAGGAATCAGTAGTTGTAAGACTTCCAACCGTTGGAGCCATGAAGAAGACAGTGCTCCTATTAGGTTGGCAGGATCTATATTATGTTTGAAAGCTGTAGGAGATGAACTCTCTCCAATTCTCTCTCAAGACTGCTCGAGCCAACAAAGTGTTTGGAAATATGCCTCAAATGCCAAGCTACAACTAGCCACCATCGATAAACAAGGACAGACCTTATGCTTGCAAAGAGCTTCTCATACCCATCAAATCGTGACTAATAAGTGCATGTGTTTGAATGATTCTCAATGTCAAGAAGATCCACAAAGCCAATGGTTTACTCTTGTTCCATCAAATGTACGTCTCATTTAA

Coding sequence (CDS)

ATGGTTGCTCTTAACATAGATCTGAGTTCAAATCTCACAACATCGTTCATAATGTACCATCCTCTTAGTGGTGGATGTGTGCGAATGAACAAAAAATATCAATTAGGAATCAGTAGTTGTAAGACTTCCAACCGTTGGAGCCATGAAGAAGACAGTGCTCCTATTAGGTTGGCAGGATCTATATTATGTTTGAAAGCTGTAGGAGATGAACTCTCTCCAATTCTCTCTCAAGACTGCTCGAGCCAACAAAGTGTTTGGAAATATGCCTCAAATGCCAAGCTACAACTAGCCACCATCGATAAACAAGGACAGACCTTATGCTTGCAAAGAGCTTCTCATACCCATCAAATCGTGACTAATAAGTGCATGTGTTTGAATGATTCTCAATGTCAAGAAGATCCACAAAGCCAATGGTTTACTCTTGTTCCATCAAATGTACGTCTCATTTAA

Protein sequence

MVALNIDLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKAVGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLNDSQCQEDPQSQWFTLVPSNVRLI

Homology

[Close]

BLAST of Lsi09G005610 vs. ExPASy TrEMBL
Match: A0A5D3BML8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00620 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.7e-68
Identity = 125/143 (87.41%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 378 DSSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 437

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY S+AKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 438 IGVGLPPILSQDCSSQQSIWKYGSDAKLQLATVDEQGQVLCLQRASHSHQIVTNKCLCSN 497

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 498 DSQCQEDPQSQWFTLVPSNLRLI 520

[Close]

BLAST of Lsi09G005610 vs. ExPASy TrEMBL
Match: A0A5A7UKP6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00780 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.7e-68
Identity = 125/143 (87.41%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 378 DSSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 437

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY S+AKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 438 IGVGLPPILSQDCSSQQSIWKYGSDAKLQLATVDEQGQVLCLQRASHSHQIVTNKCLCSN 497

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 498 DSQCQEDPQSQWFTLVPSNLRLI 520

[Close]

BLAST of Lsi09G005610 vs. ExPASy TrEMBL
Match: A0A5A7UGS0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00740 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.9e-68
Identity = 125/143 (87.41%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 378 DPSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 437

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY SNAKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 438 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 497

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+R I
Sbjct: 498 DSQCQEDPQSQWFTLVPSNLRRI 520

[Close]

BLAST of Lsi09G005610 vs. ExPASy TrEMBL
Match: A0A5D3CGT4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold66G00030 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.8e-68
Identity = 124/143 (86.71%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSN TTSFIMYHPLSGGC+RMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 352 DPSSNFTTSFIMYHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 411

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY SNAKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 412 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 471

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 472 DSQCQEDPQSQWFTLVPSNLRLI 494

[Close]

BLAST of Lsi09G005610 vs. ExPASy TrEMBL
Match: A0A5A7TI19 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00580 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.8e-68
Identity = 124/143 (86.71%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSN TTSFIMYHPLSGGC+RMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 396 DPSSNFTTSFIMYHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 455

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY SNAKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 456 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 515

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 516 DSQCQEDPQSQWFTLVPSNLRLI 538

[Close]

BLAST of Lsi09G005610 vs. NCBI nr
Match: KAA0055147.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 268.5 bits (685), Expect = 3.5e-68
Identity = 125/143 (87.41%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 378 DSSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 437

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY S+AKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 438 IGVGLPPILSQDCSSQQSIWKYGSDAKLQLATVDEQGQVLCLQRASHSHQIVTNKCLCSN 497

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 498 DSQCQEDPQSQWFTLVPSNLRLI 520

[Close]

BLAST of Lsi09G005610 vs. NCBI nr
Match: TYK00315.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 268.5 bits (685), Expect = 3.5e-68
Identity = 125/143 (87.41%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 378 DSSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 437

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY S+AKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 438 IGVGLPPILSQDCSSQQSIWKYGSDAKLQLATVDEQGQVLCLQRASHSHQIVTNKCLCSN 497

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 498 DSQCQEDPQSQWFTLVPSNLRLI 520

[Close]

BLAST of Lsi09G005610 vs. NCBI nr
Match: KAA0055143.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 267.7 bits (683), Expect = 6.0e-68
Identity = 125/143 (87.41%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 378 DPSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 437

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY SNAKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 438 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 497

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+R I
Sbjct: 498 DSQCQEDPQSQWFTLVPSNLRRI 520

[Close]

BLAST of Lsi09G005610 vs. NCBI nr
Match: TYK11103.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 267.3 bits (682), Expect = 7.9e-68
Identity = 124/143 (86.71%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSN TTSFIMYHPLSGGC+RMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 352 DPSSNFTTSFIMYHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 411

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY SNAKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 412 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 471

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 472 DSQCQEDPQSQWFTLVPSNLRLI 494

[Close]

BLAST of Lsi09G005610 vs. NCBI nr
Match: XP_008459280.1 (PREDICTED: uncharacterized protein LOC103498458 [Cucumis melo])

HSP 1 Score: 267.3 bits (682), Expect = 7.9e-68
Identity = 124/143 (86.71%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 7   DLSSNLTTSFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKA 66
           D SSN TTSFIMYHPLSGGC+RMNKKYQLGISSCKTSNRWSHE+D API+LAGSILCLKA
Sbjct: 576 DPSSNFTTSFIMYHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 635

Query: 67  VGDELSPILSQDCSSQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCLN 126
           +G  L PILSQDCSSQQS+WKY SNAKLQLAT+D+QGQ LCLQRASH+HQIVTNKC+C N
Sbjct: 636 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 695

Query: 127 DSQCQEDPQSQWFTLVPSNVRLI 150
           DSQCQEDPQSQWFTLVPSN+RLI
Sbjct: 696 DSQCQEDPQSQWFTLVPSNLRLI 718

[Close]

BLAST of Lsi09G005610 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 84.3 bits (207), Expect = 8.8e-17
Identity = 53/133 (39.85%), Postives = 69/133 (51.88%), Query Frame = 0


Query: 17  IMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEEDSAPIRLAGSILCLKAVGDELSPILS 76
           IM+HP +G C+     +QL + SC  S  W              ILCLKA     S  L 
Sbjct: 397 IMFHPSTGLCIVRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLR 456

Query: 77  QDCS-SQQSVWKYASNAKLQLATIDKQGQTLCLQRASHTHQIVTNKCMCL-NDSQCQEDP 136
              S S  S WK  S++K+QL++I K G ++CL   +  + IVTN C CL  +S C  DP
Sbjct: 457 LFFSESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTENNNIVTNSCKCLRGNSSC--DP 516

Query: 137 QSQWFTLVPSNVR 148
           +SQWF LV S  R
Sbjct: 517 RSQWFKLVTSTRR 527

The following BLAST results are available for this feature:

BLAST of Lsi09G005610 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi09G005610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BML8	1.7e-68	87.41	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UKP6	1.7e-68	87.41	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7UGS0	2.9e-68	87.41	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3CGT4	3.8e-68	86.71	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7TI19	3.8e-68	86.71	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of Lsi09G005610 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0055147.1	3.5e-68	87.41	endoglucanase-like [Cucumis melo var. makuwa]	[more]
TYK00315.1	3.5e-68	87.41	endoglucanase-like [Cucumis melo var. makuwa]	[more]
KAA0055143.1	6.0e-68	87.41	endoglucanase-like [Cucumis melo var. makuwa]	[more]
TYK11103.1	7.9e-68	86.71	endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_008459280.1	7.9e-68	86.71	PREDICTED: uncharacterized protein LOC103498458 [Cucumis melo]	[more]

BLAST of Lsi09G005610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26130.1	8.8e-17	39.85	Cellulase (glycosyl hydrolase family 5) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31263:SF50	HYDROLYZING O-GLYCOSYL COMPOUNDS HYDROLASE	coord: 5..146
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 5..146
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 15..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G005610.1	Lsi09G005610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity