Lsi09G005270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G005270
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	chr09: 5550017 .. 5553868 (+)
RNA-Seq Expression	Lsi09G005270
Synteny	Lsi09G005270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATACCCCATTTCTCCTTTGTAGGAAAGAAAAACCAATGGGAAACACTACTCAAACCAGCTTTGTGTTTTTAGCTTTCATCTCTTTCTTTTCATCTCTTTCTTATTCTTTACCTCTTTCCACGAACGGGAGATGGATTGTCGATTCGGCCACTGGACGTCGAGTGAAGCTGGTGTGCGTGAATTGGCCTTCTCACACTCAGAGCATGCTAATCGAAGGTCTTGATCGTAGGCCATTGAAAGACCTTGCTGATGAGGCGGTTCGGTTGAGGTTTAATTGTGTGAGGCTTACGTATGCAACTCACATGTTTACTCGATTTGCTAATAGGACAGTTGAAGAGAATTTTGACCTTCTTGATTTGAAAGCTTCCAAGGCGGGGTTGGCTTTGCATAATCCATTTGTATTGAACATGACTATTTTTGAAGCTTATGAAGCTGCGGTTGATGTGCTTGGGACTAGTGGTTTGATGGTTATAGCTGATAATCATATTAGCCAACCAAAATGGTGTTGCTCTCTTGACGATGGAAATGGTTTCTTTGGAGATCGCTATTTCGACCCGGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCTCGACGATTTTGCAACAAATCAACTGTATGTAAATGTTGTCCTTTTGAAATGATTTTCTGAAATATGAAACAAATAACAAAGAGATACACATACAGGGAGATTTTTAAAAATAGAAAAATAAGGAAAATTATTTACACAAAATAGCAAATTTTTTAATAGTTGTGATAGACGCTGATAGAAGTCTATCAGAGTCTATCAGTGATAGAAATGATAGAAGTCTATCACTGATAGATGCTGATAGAAGTCTATCAATATGTCTATCAGTATTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGACTTTAACTTTACGTCAAACAGGTGGTAGCCATGAGCCTACGAAACGAACTTCGAGGCGCAAAATCAAAATCGAAAGACTGGAATAAATACATGACACAAGGAGCAACAACAATTCACAACATAAATCTGAACATCCTCGTAATCATTTCAGGTCTAAACTTCGACAATGACCTACGATGCCAAAGACAAAACCCCTTGCCACTAAACAACCTACACAACAAGCTAGTTTTTGAAGTACACTTATATTCCTTCAGTGGAGAGTCCCAATCAAAGTTCATCCATAATCCTCTCAACAAAATCTGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAGTTTGGGTTTGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAATGCTCATCTTGCAAAGAAAGATTTGGATTGGGCATTATGGTCTTGGCAAGGGAGTTATTATTATAGACAAGGCAAAGTCGAACCTGAAGAAGTGTTTGGAGTTTTGAATTATAATTGGACCGAGGTTAGAAACCCTAATTTTCCCCAGATATTTCAGCTCTTGCAAACCATGTTGCAAGGTATGGCTTCCAGTTCTGTTTGATAACGATTTTGTTTTGCTTTTTCATTTTTACATTTAGTTTTTATACTAATTTTTTAAATTAAAGTATTAGAATTATTAGTATTAAAATAATAATGATATGACTCTAAAGATGGTCAATAGGAAGTCATAACAAACAAATAAGGGTCCATTTAGATTAAATTGAGAAAAAAGTGTTTTTTAAAAAACTCATTTTTATTTACATTCTTTTGATAAAAAATTGTTTAAAATACACTTCAAAAGTTATTTTGAGTAGTTGTCAAACACTCAAGTTTTTTTTTCGAAATGACTTATTTTTTAACTTGAAAATGTATTCCAAACACACTTAAAAAAATTAAGGGAAATTTTCACATATAGAAAAAATGTCAAACTATTTACAGAAAATAGCAAAAAAAAACACTAATAGACATTGATAGACTTCTATCAACATCTATCAGTGATATATTTCTATCATTTCTATCACTGATAGACCCTCATAGACTTCTATCAACATCTATCACAACTATAAAAGAATTTGCTCTTTTATGTAAATAGTTTCCCTTATTTTTCTATTTTTAAAAATTACCCATTTTTTAATTAGTATGAAATTGAAAACTATATATAGCTAGCCTTCTCTTTTTCCTTTTTTTTTTTTTTTTTTTTGGATAATTACTTCATATATTCACATGCAGATCCAAAGTCCAATTCCTCAAACACTTATGTAATGTATCATCCACAAAGTGGGCAATGTGTCAAAGTGAAAGACAATATGGATAAGGAAATTTATCTCAACAATTGTTCCAATGCAAGTCATTGGAGCCATGAAGGAGATGGGGCTCCAATAATGTTGGAAGCCACTGATTTGTGTCTAAAAGCCAATGGAAATGGGGTTCCACCATCACTCTCAAGGGATTGTTTTGATGAACAAAGCGGTTGGACAGCCATTTCAGACTCTAAGCTTCATTTGGCAACACTAACAAAAGAAGGGAATAATAATAATAATGGTTTGTGTTTAGAGAAAGAGAGCTCAAATTCAACTAAGATTGTGATGGGGAGATGTGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTGAACTTGTTGTTACAAATACTTTGTAGTATTTTACCATATGGGGAGGAAACAAGAAAAAAAAATTAAGAAGGACCGTTCTCTATCGAAATTCTGTAAGGCCCCTAGTTAGGAAAATAATAGGATTATTAGTAGAATATTAATATAGTTATTAGAAGGGGCAAATTAGTAAATAGCTAGTTAGTCTGGTTATGGTTTTAGTTATAAATAGGAGTGAGTAGGAAGGGTGTGGAGAATTTGATAGTGATTTTCGGTGGGAAATATTGAGAGAGAGTCGGCCTCTCGCAAAAGGTTGTGGTATATTGTAGTTTTTTTCATTGATATTACAATATAAATTTATCTCTTAGTGTTCTTTGTTT

mRNA sequence

GAATACCCCATTTCTCCTTTGTAGGAAAGAAAAACCAATGGGAAACACTACTCAAACCAGCTTTGTGTTTTTAGCTTTCATCTCTTTCTTTTCATCTCTTTCTTATTCTTTACCTCTTTCCACGAACGGGAGATGGATTGTCGATTCGGCCACTGGACGTCGAGTGAAGCTGGTGTGCGTGAATTGGCCTTCTCACACTCAGAGCATGCTAATCGAAGGTCTTGATCGTAGGCCATTGAAAGACCTTGCTGATGAGGCGGTTCGGTTGAGGTTTAATTGTGTGAGGCTTACGTATGCAACTCACATGTTTACTCGATTTGCTAATAGGACAGTTGAAGAGAATTTTGACCTTCTTGATTTGAAAGCTTCCAAGGCGGGGTTGGCTTTGCATAATCCATTTGTATTGAACATGACTATTTTTGAAGCTTATGAAGCTGCGGTTGATGTGCTTGGGACTAGTGGTTTGATGGTTATAGCTGATAATCATATTAGCCAACCAAAATGGTGTTGCTCTCTTGACGATGGAAATGGTTTCTTTGGAGATCGCTATTTCGACCCGGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCTCGACGATTTTGCAACAAATCAACTGTGGTAGCCATGAGCCTACGAAACGAACTTCGAGGCGCAAAATCAAAATCGAAAGACTGGAATAAATACATGACACAAGGAGCAACAACAATTCACAACATAAATCTGAACATCCTCGTAATCATTTCAGGTCTAAACTTCGACAATGACCTACGATGCCAAAGACAAAACCCCTTGCCACTAAACAACCTACACAACAAGCTAGTTTTTGAAGTACACTTATATTCCTTCAGTGGAGAGTCCCAATCAAAGTTCATCCATAATCCTCTCAACAAAATCTGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAGTTTGGGTTTGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAATGCTCATCTTGCAAAGAAAGATTTGGATTGGGCATTATGGTCTTGGCAAGGGAGTTATTATTATAGACAAGGCAAAGTCGAACCTGAAGAAGTGTTTGGAGTTTTGAATTATAATTGGACCGAGGTTAGAAACCCTAATTTTCCCCAGATATTTCAGCTCTTGCAAACCATGTTGCAAGATCCAAAGTCCAATTCCTCAAACACTTATGTAATGTATCATCCACAAAGTGGGCAATGTGTCAAAGTGAAAGACAATATGGATAAGGAAATTTATCTCAACAATTGTTCCAATGCAAGTCATTGGAGCCATGAAGGAGATGGGGCTCCAATAATGTTGGAAGCCACTGATTTGTGTCTAAAAGCCAATGGAAATGGGGTTCCACCATCACTCTCAAGGGATTGTTTTGATGAACAAAGCGGTTGGACAGCCATTTCAGACTCTAAGCTTCATTTGGCAACACTAACAAAAGAAGGGAATAATAATAATAATGGTTTGTGTTTAGAGAAAGAGAGCTCAAATTCAACTAAGATTGTGATGGGGAGATGTGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTGAACTTGTTGTTACAAATACTTTGTAGTATTTTACCATATGGGGAGGAAACAAGAAAAAAAAATTAAGAAGGACCGTTCTCTATCGAAATTCTGTAAGGCCCCTAGTTAGGAAAATAATAGGATTATTAGTAGAATATTAATATAGTTATTAGAAGGGGCAAATTAGTAAATAGCTAGTTAGTCTGGTTATGGTTTTAGTTATAAATAGGAGTGAGTAGGAAGGGTGTGGAGAATTTGATAGTGATTTTCGGTGGGAAATATTGAGAGAGAGTCGGCCTCTCGCAAAAGGTTGTGGTATATTGTAGTTTTTTTCATTGATATTACAATATAAATTTATCTCTTAGTGTTCTTTGTTT

Coding sequence (CDS)

ATGGGAAACACTACTCAAACCAGCTTTGTGTTTTTAGCTTTCATCTCTTTCTTTTCATCTCTTTCTTATTCTTTACCTCTTTCCACGAACGGGAGATGGATTGTCGATTCGGCCACTGGACGTCGAGTGAAGCTGGTGTGCGTGAATTGGCCTTCTCACACTCAGAGCATGCTAATCGAAGGTCTTGATCGTAGGCCATTGAAAGACCTTGCTGATGAGGCGGTTCGGTTGAGGTTTAATTGTGTGAGGCTTACGTATGCAACTCACATGTTTACTCGATTTGCTAATAGGACAGTTGAAGAGAATTTTGACCTTCTTGATTTGAAAGCTTCCAAGGCGGGGTTGGCTTTGCATAATCCATTTGTATTGAACATGACTATTTTTGAAGCTTATGAAGCTGCGGTTGATGTGCTTGGGACTAGTGGTTTGATGGTTATAGCTGATAATCATATTAGCCAACCAAAATGGTGTTGCTCTCTTGACGATGGAAATGGTTTCTTTGGAGATCGCTATTTCGACCCGGAAGAATGGTTGGAAGGTCTTCGTTTGGTTGCTCGACGATTTTGCAACAAATCAACTGTGGTAGCCATGAGCCTACGAAACGAACTTCGAGGCGCAAAATCAAAATCGAAAGACTGGAATAAATACATGACACAAGGAGCAACAACAATTCACAACATAAATCTGAACATCCTCGTAATCATTTCAGGTCTAAACTTCGACAATGACCTACGATGCCAAAGACAAAACCCCTTGCCACTAAACAACCTACACAACAAGCTAGTTTTTGAAGTACACTTATATTCCTTCAGTGGAGAGTCCCAATCAAAGTTCATCCATAATCCTCTCAACAAAATCTGCTCAAAGATCATCAATGGGTTTGTGGAGAGAGCTGAGTTTGTGATGGAAGGAGCTGAGGCAGTTCCTTTGTTTGTGAGTGAGTTTGGGTTTGACCAAAGGGGAGTTAATGAGGCTGATGATAGGTTTTTGAGTTGCTTTAATGCTCATCTTGCAAAGAAAGATTTGGATTGGGCATTATGGTCTTGGCAAGGGAGTTATTATTATAGACAAGGCAAAGTCGAACCTGAAGAAGTGTTTGGAGTTTTGAATTATAATTGGACCGAGGTTAGAAACCCTAATTTTCCCCAGATATTTCAGCTCTTGCAAACCATGTTGCAAGATCCAAAGTCCAATTCCTCAAACACTTATGTAATGTATCATCCACAAAGTGGGCAATGTGTCAAAGTGAAAGACAATATGGATAAGGAAATTTATCTCAACAATTGTTCCAATGCAAGTCATTGGAGCCATGAAGGAGATGGGGCTCCAATAATGTTGGAAGCCACTGATTTGTGTCTAAAAGCCAATGGAAATGGGGTTCCACCATCACTCTCAAGGGATTGTTTTGATGAACAAAGCGGTTGGACAGCCATTTCAGACTCTAAGCTTCATTTGGCAACACTAACAAAAGAAGGGAATAATAATAATAATGGTTTGTGTTTAGAGAAAGAGAGCTCAAATTCAACTAAGATTGTGATGGGGAGATGTGTTTGTGTTGGTAATGATTCAAATTGTTTACAAGATACTCAAGCTCAATGGTTTGAACTTGTTGTTACAAATACTTTGTAG

Protein sequence

MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNPFVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKVEPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNMDKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISDSKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTNTL

Homology

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BLAST of Lsi09G005270 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.0e-115
Identity = 225/532 (42.29%), Postives = 315/532 (59.21%), Query Frame = 0


Query: 22  SYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLADEAVRLRFNC 81
           S+SLPL T GRWIVD ATG RVKL CVNW  H +  L EGL+R P+  +A     L FNC
Sbjct: 26  SHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVATVAHTISSLGFNC 85

Query: 82  VRLTYATHMFTR--FANRTVEENFDLLDLKASKAGLALHNPFVLNMTIFEAYEAAVDVLG 141
           VRLTY+ HM TR  + N TV + F  L+L  + +G+  +NP +L++    AY   V  L 
Sbjct: 86  VRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLGHVAAYHHVVAALS 145

Query: 142 TSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFCNKSTVVAMSL 201
            +G+MVI DNH+S+PKWCC++DDGNGFFGDRYF+P  W+EGL L+A  F N   VVAMSL
Sbjct: 146 EAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMATYFNNTPNVVAMSL 205

Query: 202 RNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDNDLRCQRQNPLPLNNLHN 261
           RNELRG +S    W+++M  GA T+H  N  +LVI+SGL FD DL      P+ L     
Sbjct: 206 RNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSFLPVLPVTL-PFKE 265

Query: 262 KLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVMEGAE--AVPLFVSEFGF 321
           K+V+E H YSF    ++       N +C      F     FV   A   A PLF+SEFG 
Sbjct: 266 KIVYEGHWYSFGVPWRTGL----PNDVCKNETGRFKSNVGFVTSSANATAAPLFMSEFGI 325

Query: 322 DQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGK---VEPEEVFGVLNYNWT 381
           DQR VN+ D+R+L+C  A+LA++DLDWALW+  GSYYYR  K    + EE +G  N++W+
Sbjct: 326 DQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKDFEETYGFFNHDWS 385

Query: 382 EVRNPNFPQIFQLLQTMLQDPKSNSSNTY-VMYHPQSGQCVKVKDNMDKEIYLNNCSNA- 441
            +RNP+F    + +Q  +QDP       Y ++YHP SG C  V+  +   ++L +C +  
Sbjct: 386 RIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLC--VESGIGNTVHLGSCQSVR 445

Query: 442 SHWSHEGD-GAPIMLEATDLCLKANGNGVPPSLSRDC-FDEQSGWTAISDSKLHLATLTK 501
           S W+++     PI L  +  C+   GNG+P  ++ +C     + W+ +S ++L L T   
Sbjct: 446 SRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRVL 505

Query: 502 EGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQ--DTQAQWFELVVTN 541
             +     +CL  + S S  I    C+C+  DS+C    + + QWF+++ TN
Sbjct: 506 GKDGKEKWMCL--DGSKSPLISTNECICI-TDSHCYPKLNPEKQWFKVITTN 547

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BLAST of Lsi09G005270 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 3.9e-11
Identity = 92/377 (24.40%), Postives = 148/377 (39.26%), Query Frame = 0


Query: 20  SLSYSLPLSTNGRWIVDSATGRRVKLVCVN-WPSHTQSMLIEGLDRRPLKDLADEAVRLR 79
           + SYS+    N R IVD  +G+ V+L  VN +   T + ++ GL  R  KD+  +   L 
Sbjct: 23  AFSYSI---NNSRQIVDD-SGKVVQLKGVNVFGFETGNHVMHGLWARNWKDMIVQMQGLG 82

Query: 80  FNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNPFVLNMTIFEAYEAAVDVL 139
           FN VRL +         + T+  + D              N  +  +T  +  +  +   
Sbjct: 83  FNAVRLPFCP---ATLRSDTMPASIDY-----------SRNADLQGLTSLQILDKVIAEF 142

Query: 140 GTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFCNKSTVVAMS 199
              G+ V+ D+H      C  + +    +    +   +WL  LR VA R+ N   V+ + 
Sbjct: 143 NARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQWLADLRFVANRYKNVPYVLGLD 202

Query: 200 LRNELRGAK-----SKSKDWNKYMTQGATTIHNINLNILVIISGLNFDNDLRCQRQ---- 259
           L+NE  GA      + + DWNK   +G+  +  +    L+ + G+  DN + C       
Sbjct: 203 LKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGIT-DNPV-CSTNGGIF 262

Query: 260 -----NPL---PLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFV 319
                 PL   PLN   N+L+   H+Y      QS F  N  N      +    ER    
Sbjct: 263 WGGNLQPLACTPLNIPANRLLLAPHVYGPDVFVQSYF--NDSN--FPNNMPAIWERHFGQ 322

Query: 320 MEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWAL-WSWQGSYYYRQGKV 378
             G  A  L + EFG      +  D  +      +L  K ++    WSW  +        
Sbjct: 323 FAGTHA--LLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGFYWSWNPN-------- 358

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BLAST of Lsi09G005270 vs. ExPASy TrEMBL
Match: A0A5A7UH07 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00380 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 479/542 (88.38%), Postives = 507/542 (93.54%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG TTQ SFV LAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GLDRRPLKDLA+E +RLRFNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASK GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRGA SKSKDWNKY+TQGATTIHNIN NILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF+AHL +KDLDWALW WQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           E EEVFGVLNYNW++VRNP F Q+FQLLQTMLQDP SNSSNTY+MYHPQSGQCV+V D  
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            KEI+L+NCSNASHWS+EGDG PIML +T+ CLKANGNG+PPSLSRDCF EQS WTAISD
Sbjct: 421 KKEIFLDNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAISD 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLTK+G   NNG+CLEKESSNS++I+M  CVCVG+DSNCLQDTQAQWF+LVVTN
Sbjct: 481 SKLHLATLTKQG---NNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

Query: 541 TL 543
           TL
Sbjct: 541 TL 539

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BLAST of Lsi09G005270 vs. ExPASy TrEMBL
Match: A0A1S3CTF8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.1e-286
Identity = 477/542 (88.01%), Postives = 505/542 (93.17%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG TTQ SFV LAFI FFSSLSYSLPLSTNGRWIVDSATG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GLDRRPLKDLA+E +RL+FNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASK GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRGA SKSKDWNKY+TQGATTIHNIN NILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF+AHL +KDLDWALW WQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           E EEVFGVLNYNW++VRNP F Q+FQLLQTMLQDP SNSSNTY+MYHPQSGQCV+V D  
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            KEI+LNNCSNASHWS+EGDG PIML +T+ CLKANGNG+PPSLSRDCF EQS WTAISD
Sbjct: 421 QKEIFLNNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAISD 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLTK+G   NNG+CLEKESSNS++I+M  CVCVG+DSNCLQDTQAQWF+LVVTN
Sbjct: 481 SKLHLATLTKQG---NNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

Query: 541 TL 543
           TL
Sbjct: 541 TL 539

[Close]

BLAST of Lsi09G005270 vs. ExPASy TrEMBL
Match: A0A6J1H201 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.9e-269
Identity = 448/542 (82.66%), Postives = 487/542 (89.85%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG+ +QT  + LAFIS FSSLS SLPLST GRWIVDS TGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGSPSQT-LMLLAFISLFSSLSNSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GL  RPLKDLADE V L+FNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASK GLA HNP
Sbjct: 61  GLANRPLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLASHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYE  VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFDP+EWLEG
Sbjct: 121 FVLNMTIFEAYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRGAKS SKDWNKYMTQGATTIH+IN N+LVI+SGLNF
Sbjct: 181 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQR NPLPLNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS IINGFVERAEF
Sbjct: 241 DNDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VM+GAEAVPLFVSEFGFDQRG N ADDRFLSCF AHLAK DLDWALW+WQGSYYYRQG+ 
Sbjct: 301 VMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQA 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           + +EVFG+LNYNW+ VRNP F + FQLLQTML+DP SN+ N+YVMYHPQSGQCV+VKD M
Sbjct: 361 QFDEVFGILNYNWSGVRNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMM 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            KEIYLN+CSNASHWSH GDG PI LEAT LCLKA+G+G+ P LSRDC   +S WT IS+
Sbjct: 421 SKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCLSNESSWTTISN 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLT+ G    NGLCLEK+SSNST+IVMGRCVCVG+DSNCL DT++QWFELVVTN
Sbjct: 481 SKLHLATLTRHG----NGLCLEKDSSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVVTN 537

Query: 541 TL 543
           TL
Sbjct: 541 TL 537

[Close]

BLAST of Lsi09G005270 vs. ExPASy TrEMBL
Match: A0A6J1K1F7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.5e-268
Identity = 445/542 (82.10%), Postives = 490/542 (90.41%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG+ TQT F+ LAFIS FSSLS SLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGSPTQT-FMLLAFISLFSSLSNSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GL  RPLKDLADE V L+FNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASKAGLA HNP
Sbjct: 61  GLAHRPLKDLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMTIF+AYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFDP+EWLEG
Sbjct: 121 FILNMTIFDAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NK  VVAMSLRNELRGAKS SKDWNKYMTQGATTIH+IN N+LVI+SGLNF
Sbjct: 181 LRLVARRFTNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQR NPLPLNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS IINGFVERAEF
Sbjct: 241 DNDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VM+GAEAVPLFVSEFGFDQRG N ADDRF SCF AHLA+ DLDWALW+WQGSYYYRQG+ 
Sbjct: 301 VMQGAEAVPLFVSEFGFDQRGTNVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQA 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           + +EVFGVLNYNW++VRNP+F + FQLLQTML+DP SN+ N+YVMYHPQSGQCV+VKD M
Sbjct: 361 QSDEVFGVLNYNWSDVRNPHFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMM 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            K+IYLN+CSNASHWSH GDG PI LEAT LCLKA+ +G+ P LSRDC  ++S WT IS+
Sbjct: 421 SKDIYLNDCSNASHWSHRGDGTPIELEATSLCLKADRDGLRPLLSRDCSGDESAWTTISN 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLAT T++G    NGLCLEKESSNST+IVMGRC+CVG+DSNCL DT++QWFELV TN
Sbjct: 481 SKLHLATFTRQG----NGLCLEKESSNSTRIVMGRCLCVGDDSNCLDDTRSQWFELVGTN 537

Query: 541 TL 543
           TL
Sbjct: 541 TL 537

[Close]

BLAST of Lsi09G005270 vs. ExPASy TrEMBL
Match: A0A6J1DXU9 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 434/543 (79.93%), Postives = 476/543 (87.66%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG+T +T FVFLA IS  SSLSYSLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGSTKET-FVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTR+ANRT+EENFDLLDLKASKAGLALHNP
Sbjct: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYEAAVDVLG SGLMVIADNHISQP+WCCSLDDGNGFFGDRYFDPEEWLEG
Sbjct: 121 FVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVAR F NKS+VV MSLRNELRGA+S  KDWNK++T+GA  IH IN N LVIISGLN+
Sbjct: 181 LRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNY 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQ Q PLPL NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFVERA F
Sbjct: 241 DNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGF 300

Query: 301 VMEGAEAVPLFVSEFGFDQR-GVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGK 360
           V+EG  AVPL VSEFG DQR   NEADDRF+SCF A+LA+ DLDWA+W WQGSYYYRQG+
Sbjct: 301 VVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQ 360

Query: 361 VEPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDN 420
             P EVFGVLNY+W+ VRNP F Q FQLLQTMLQDP S S N+Y+MYHPQSGQCV+ + N
Sbjct: 361 ARPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQDPNSYSPNSYLMYHPQSGQCVQAEGN 420

Query: 421 MDKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAIS 480
             KEIY+NNCS+AS WSHEGDG PI L+ + LCL+A+G G+ PSLS +C  + SGW AIS
Sbjct: 421 -SKEIYMNNCSHASQWSHEGDGTPIRLKDSGLCLRASGYGLKPSLSTECLYKHSGWRAIS 480

Query: 481 DSKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVT 540
           DSKLHLATL   GN N N LCLE+ESSNST+IVMGRC+CVG+DS+CL DTQAQWF+LVVT
Sbjct: 481 DSKLHLATLAPNGNGNGN-LCLERESSNSTRIVMGRCICVGDDSSCLHDTQAQWFQLVVT 540

Query: 541 NTL 543
           NTL
Sbjct: 541 NTL 540

[Close]

BLAST of Lsi09G005270 vs. NCBI nr
Match: XP_038875504.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 1030.0 bits (2662), Expect = 7.3e-297
Identity = 495/541 (91.50%), Postives = 516/541 (95.38%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MGNTTQ +FVFLAFISFF SLSYS PLSTNGRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGNTTQINFVFLAFISFF-SLSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GLDRRPLKDLADE VRLRFNCVRLTYATHMFTR+ANRTV+ENFDLLDLKASK GLALHNP
Sbjct: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           F+LNMT+F AYEAAVD+LGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG
Sbjct: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRG KSKSKDWNKYMTQGATTIH IN NIL+IISGLNF
Sbjct: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFI+NPLNKICSKIINGFVERAEF
Sbjct: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCF AHLAKKDLDWALW+WQGSYYYRQGK+
Sbjct: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGKI 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           EPEEVFGVLNYNWT+VRNP+F Q+FQLLQTMLQDP SNSSNTYVMYHPQSGQCV+VKD M
Sbjct: 361 EPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVQVKDMM 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
           DK+IYLNNCSNASHWSHEGDG PIMLEAT+ CLKANGNG+PPSLS DCF EQS WTAISD
Sbjct: 421 DKQIYLNNCSNASHWSHEGDGTPIMLEATNFCLKANGNGLPPSLSSDCFGEQSVWTAISD 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLTK+G   NNGLCLEKESSNST+IVMG CVCVGNDSNCLQDTQAQWFELVVTN
Sbjct: 481 SKLHLATLTKQG---NNGLCLEKESSNSTRIVMGSCVCVGNDSNCLQDTQAQWFELVVTN 536

Query: 541 T 542
           T
Sbjct: 541 T 536

[Close]

BLAST of Lsi09G005270 vs. NCBI nr
Match: KAA0055112.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 994.6 bits (2570), Expect = 3.4e-286
Identity = 479/542 (88.38%), Postives = 507/542 (93.54%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG TTQ SFV LAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GLDRRPLKDLA+E +RLRFNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASK GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRGA SKSKDWNKY+TQGATTIHNIN NILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF+AHL +KDLDWALW WQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           E EEVFGVLNYNW++VRNP F Q+FQLLQTMLQDP SNSSNTY+MYHPQSGQCV+V D  
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            KEI+L+NCSNASHWS+EGDG PIML +T+ CLKANGNG+PPSLSRDCF EQS WTAISD
Sbjct: 421 KKEIFLDNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSIWTAISD 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLTK+G   NNG+CLEKESSNS++I+M  CVCVG+DSNCLQDTQAQWF+LVVTN
Sbjct: 481 SKLHLATLTKQG---NNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

Query: 541 TL 543
           TL
Sbjct: 541 TL 539

[Close]

BLAST of Lsi09G005270 vs. NCBI nr
Match: XP_008467306.1 (PREDICTED: major extracellular endoglucanase-like [Cucumis melo])

HSP 1 Score: 992.3 bits (2564), Expect = 1.7e-285
Identity = 477/542 (88.01%), Postives = 505/542 (93.17%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG TTQ SFV LAFI FFSSLSYSLPLSTNGRWIVDSATG RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGITTQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GLDRRPLKDLA+E +RL+FNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASK GLALHNP
Sbjct: 61  GLDRRPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRGA SKSKDWNKY+TQGATTIHNIN NILVIISGLNF
Sbjct: 181 LRLVARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFV+RAEF
Sbjct: 241 DNDLRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VMEGAEAVPLFVSEFG DQ GVNEADDRFLSCF+AHL +KDLDWALW WQGSYYYRQGKV
Sbjct: 301 VMEGAEAVPLFVSEFGLDQTGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKV 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           E EEVFGVLNYNW++VRNP F Q+FQLLQTMLQDP SNSSNTY+MYHPQSGQCV+V D  
Sbjct: 361 ELEEVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNSNSSNTYLMYHPQSGQCVQVHDMK 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            KEI+LNNCSNASHWS+EGDG PIML +T+ CLKANGNG+PPSLSRDCF EQS WTAISD
Sbjct: 421 QKEIFLNNCSNASHWSYEGDGTPIMLASTNFCLKANGNGLPPSLSRDCFGEQSVWTAISD 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLTK+G   NNG+CLEKESSNS++I+M  CVCVG+DSNCLQDTQAQWF+LVVTN
Sbjct: 481 SKLHLATLTKQG---NNGMCLEKESSNSSRILMRSCVCVGSDSNCLQDTQAQWFQLVVTN 539

Query: 541 TL 543
           TL
Sbjct: 541 TL 539

[Close]

BLAST of Lsi09G005270 vs. NCBI nr
Match: XP_004143723.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical protein Csa_000506 [Cucumis sativus])

HSP 1 Score: 987.6 bits (2552), Expect = 4.1e-284
Identity = 474/539 (87.94%), Postives = 503/539 (93.32%), Query Frame = 0


Query: 4   TTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD 63
           TTQ  FV LAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD
Sbjct: 4   TTQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLD 63

Query: 64  RRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNPFVL 123
           RRPLKDLA+E VRLRFNCVRLTYATHMFTR+ANRTVEENFDLLDL+A+K GLA HNPFVL
Sbjct: 64  RRPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLAFHNPFVL 123

Query: 124 NMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRL 183
           NMTIFEAYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFD EEWLEGLRL
Sbjct: 124 NMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRL 183

Query: 184 VARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDND 243
           VARRF NKS VVAMSLRNELRGA SKSKDWNKY+TQGATTIHNIN  ILVIISGLNFDND
Sbjct: 184 VARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDND 243

Query: 244 LRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVME 303
           LRCQRQ PL LNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSK+INGFVERAEFVME
Sbjct: 244 LRCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVME 303

Query: 304 GAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKVEPE 363
           GAEAVPLFVSEFG DQRGVNEADDRFLSCF+AHL +KDLDWALW WQGSYYYRQGKV PE
Sbjct: 304 GAEAVPLFVSEFGLDQRGVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPE 363

Query: 364 EVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNMDKE 423
           EVFGVLNYNW++VRNP+F Q+FQLLQTMLQDP SNSSNTYVMYHPQSGQCV V+D    +
Sbjct: 364 EVFGVLNYNWSDVRNPHFSQMFQLLQTMLQDPNSNSSNTYVMYHPQSGQCVLVQDMKHMQ 423

Query: 424 IYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISDSKL 483
           IYLN+CSNASHWS+EGDG PIML +T+ CLKA+G+G+PPSLSRDCF EQS WTAISDSKL
Sbjct: 424 IYLNDCSNASHWSYEGDGTPIMLASTNFCLKASGDGLPPSLSRDCFGEQSVWTAISDSKL 483

Query: 484 HLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTNTL 543
           HLATLTK+G   NNG+CLEKESSNS++I+M  CVCVGNDSNCLQDTQAQWF+LVVTNTL
Sbjct: 484 HLATLTKQG---NNGMCLEKESSNSSRILMRSCVCVGNDSNCLQDTQAQWFQLVVTNTL 539

[Close]

BLAST of Lsi09G005270 vs. NCBI nr
Match: XP_023534098.1 (uncharacterized protein LOC111795760 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 938.3 bits (2424), Expect = 2.9e-269
Identity = 451/542 (83.21%), Postives = 488/542 (90.04%), Query Frame = 0


Query: 1   MGNTTQTSFVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 60
           MG+ TQT  + LAFIS FSSLS SLPLST GRWIVDS TGRRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGSPTQT-LMLLAFISLFSSLSNSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 61  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRFANRTVEENFDLLDLKASKAGLALHNP 120
           GL  R LKDLADE V L+FNCVRLTYATHMFTR+ANRTVEENFDLLDL+ASKAGL LHNP
Sbjct: 61  GLAHRSLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLVLHNP 120

Query: 121 FVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180
           FVLNMTIFEAYEA VDVLGTSGLMVIADNHISQP+WCCSL+DGNGFFGDRYFDPEEWLEG
Sbjct: 121 FVLNMTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPEEWLEG 180

Query: 181 LRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNF 240
           LRLVARRF NKS VVAMSLRNELRGAKS SKDWNKYMTQGATTIH+IN N+LVI+SGLNF
Sbjct: 181 LRLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNF 240

Query: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEF 300
           DNDLRCQR NPL LNNLHNKLVFEVHLYSFSG +++KFI NPLNKICS IINGFVERAEF
Sbjct: 241 DNDLRCQRHNPLLLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEF 300

Query: 301 VMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKV 360
           VM+GAEAVPLFVSEFGFDQRG N ADDRFLSCF AHLAK DLDWALW+WQGSYYYRQG+ 
Sbjct: 301 VMQGAEAVPLFVSEFGFDQRGTNVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQA 360

Query: 361 EPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM 420
           + +EVFGVLNYNW++VRNP F + FQLLQTML+DP SN+ N+YVMYHPQSGQCV+VKD M
Sbjct: 361 QSDEVFGVLNYNWSDVRNPRFSKTFQLLQTMLRDPNSNAPNSYVMYHPQSGQCVRVKDMM 420

Query: 421 DKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISD 480
            KEIYLN+CSNASHWSH GDG PI LEAT LCLKA+G+G+ P LSRDC   +S WT IS+
Sbjct: 421 SKEIYLNDCSNASHWSHRGDGTPIELEATGLCLKADGDGLRPLLSRDCSSNESSWTTISN 480

Query: 481 SKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELVVTN 540
           SKLHLATLT++G    NGLCLEKESSNST+IVMGRCVCVG+DSNCL DT++QWFELV TN
Sbjct: 481 SKLHLATLTRQG----NGLCLEKESSNSTRIVMGRCVCVGDDSNCLDDTRSQWFELVATN 537

Query: 541 TL 543
           TL
Sbjct: 541 TL 537

[Close]

BLAST of Lsi09G005270 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 420.2 bits (1079), Expect = 2.5e-117
Identity = 221/539 (41.00%), Postives = 322/539 (59.74%), Query Frame = 0


Query: 9   FVFLAFISFFSSL------SYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGL 68
           F+F  F  FFS +      + S PLST+ RWIVD   G RVKLVC NWPSH Q ++ EGL
Sbjct: 11  FIFPFFCFFFSFIAQNTVPNMSYPLSTSSRWIVDE-NGLRVKLVCANWPSHLQPVVAEGL 70

Query: 69  DRRPLKDLADEAVRLRFNCVRLTYATHMFTRFA---NRTVEENFDLLDLKASKAGLALHN 128
            ++P+  +A + V + FNCVRLT+   + T      N TV ++F  L L     G   +N
Sbjct: 71  SKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNN 130

Query: 129 PFVLNMTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLE 188
           P ++++ + EAY+  V  LG + +MVI DNH+++P WCC+ DDGNGFFGD++FDP  W+ 
Sbjct: 131 PSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVA 190

Query: 189 GLRLVARRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLN 248
            L+ +A  F   S VV MSLRNELRG K    DW KYM QGA  +H+ N  +LVI+SGL+
Sbjct: 191 ALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLS 250

Query: 249 FDNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAE 308
           FD DL   R  P+ L +   KLVFE+H YSFS +  S   +NP N IC +++N       
Sbjct: 251 FDADLSFVRSRPVKL-SFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGG 310

Query: 309 FVMEGAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGK 368
           +++   +  PLF+SEFG D+RGVN  D+R+  C     A+ D+DW+LW+  GSYY RQGK
Sbjct: 311 YLLN--QGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGK 370

Query: 369 VEPEEVFGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDN 428
           V   E +GVL+ +W  VRN +F Q    LQ+ LQ P   +    +++HP +G C+    +
Sbjct: 371 VGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLD 430

Query: 429 MDKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSR-DCFDEQSGWTAI 488
             K + L  C+++  WS+      + ++   LCL++NG   P +++R  C    S W  I
Sbjct: 431 DPKMLTLGPCNSSEPWSYTKKA--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTI 490

Query: 489 SDSKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELV 538
           S S++HLA+ T    +N   LCL+ +++N+  +V   C C+  D +C  +  +QWF+++
Sbjct: 491 SASRMHLASTT----SNKTSLCLDVDTANN--VVANACKCLSKDKSC--EPMSQWFKII 533

[Close]

BLAST of Lsi09G005270 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.4e-112
Identity = 220/543 (40.52%), Postives = 322/543 (59.30%), Query Frame = 0


Query: 9   FVFLAFISFFSSLSYSLPLSTNGRWIVDSAT-GRRVKLVCVNWPSHTQSMLIEGLDRRPL 68
           F    F+  +   +++ P ST+ RWIVD    GRRVKL CVNWPSH ++ + EGL ++PL
Sbjct: 5   FFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPL 64

Query: 69  KDLADEAVRLRFNCVRLTYATHMFTR---FANRTVEENFDLLDLKASKAGLALHNPFVLN 128
             +A++ V + FNCVRLT+  ++ T     A  TV ++     L  + +G   HNP +L+
Sbjct: 65  DAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILD 124

Query: 129 MTIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLV 188
           + + +A++  V  L    +MVI DNHISQP WCCS +DGNGFFGD++ +P+ W++GL+ +
Sbjct: 125 LPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKM 184

Query: 189 ARRFCN-KSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDND 248
           A  F N  S VV MSLRNELRG K   KDW KYM +GA  +H++N N+LVI+SGLN+  D
Sbjct: 185 ASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATD 244

Query: 249 LRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVME 308
           L   R+ P  + +   K+VFE+H Y F    +     + LNKIC K     ++ + F++E
Sbjct: 245 LSFLRERPFEV-SFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSGFLLE 304

Query: 309 GAEAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKVEPE 368
             + +PLFVSEFG DQRG N  D++FLSCF A  A +DLDW+LW+  GSYY R+  +  +
Sbjct: 305 --KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSD 364

Query: 369 EVFGVLNYNWTEVRNPNFPQIFQLLQT-----MLQDPKSNSSNTYVMYHPQSGQCVKVKD 428
           E +GVL++NW+ +RN    Q+   +QT     M   PK       +M+HP +G C+  K 
Sbjct: 365 ESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHPSTGLCIVRKS 424

Query: 429 NMDKEIYLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQ--SGWT 488
               ++ L +C+ +  W         + E   LCLKA   G    L R  F E   S W 
Sbjct: 425 LF--QLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKL-RLFFSESYCSKWK 484

Query: 489 AISDSKLHLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFEL 540
             SDSK+ L+++TK G +    +CL+ ++ N+  IV   C C+  +S+C  D ++QWF+L
Sbjct: 485 LFSDSKMQLSSITKNGFS----VCLDVDTENN-NIVTNSCKCLRGNSSC--DPRSQWFKL 524

[Close]

BLAST of Lsi09G005270 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 382.1 bits (980), Expect = 7.4e-106
Identity = 207/518 (39.96%), Postives = 297/518 (57.34%), Query Frame = 0


Query: 26  PLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLADEAVRLRFNCVRLT 85
           PLSTN RWI+D   G+RVKL CVNWPSH Q ++ EGL ++ + DLA + + + FNCVR T
Sbjct: 4   PLSTNSRWIIDE-KGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 86  YATHMFTRFA---NRTVEENFDLLDLKASKAGLALHNPFVLNMTIFEAYEAAVDVLGTSG 145
           +   + T      N TV ++F  L L    +G    NP ++++ + EAY+  V  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 146 LMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARRFCNKSTVVAMSLRNE 205
           +MVI DNH+++P WCC  +DGNGFFGD +FDP  W+ GL  +A  F   + VV MSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 206 LRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDNDLRCQRQNPLPLNNLHNKLV 265
           LRG K    DW KYM QGA  +H  N N+LVI+SGL++D DL   R   + L     KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 266 FEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVMEGAEAVPLFVSEFGFDQRGV 325
           FE+H YSF+  + +    NP N+ C +I+        F +      P+F+SEFG D RG 
Sbjct: 244 FELHRYSFT-NTNTWSSKNP-NEACGEILKSIENGGGFNLRD---FPVFLSEFGIDLRGK 303

Query: 326 NEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKVEPEEVFGVLNYNWTEVRNPNFP 385
           N  D+R++ C     A+ D+DW++W+ QGSYY R+G V   E +G+L+ +W  VR+ +F 
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 386 QIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNMDKEIYLNNCSNASHWSHEGDGA 445
           Q   L+ + LQ P S S    +++HP +G C+        ++ L  C+ +  WS+     
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 446 PIMLEATDLCLKANGNGVPPSLSR-DCFDEQ-SGWTAISDSKLHLATLTKEGNNNNNGLC 505
            + L+   LCL++ G   P  LS   C     S W  IS S + LA       + NN LC
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLA-----AKSTNNSLC 483

Query: 506 LEKESSNSTKIVMGRCVCV-GNDSNCLQDTQAQWFELV 538
           L+ + +N+  ++   C CV G DS+C  D  +QWF++V
Sbjct: 484 LDVDETNN--LMASNCKCVKGEDSSC--DPISQWFKIV 504

[Close]

BLAST of Lsi09G005270 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 8.2e-97
Identity = 199/533 (37.34%), Postives = 300/533 (56.29%), Query Frame = 0


Query: 9   FVFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLK 68
           F+FL+ IS   +L+   PL T  RWIV++  G RVKL C NWPSH + ++ EGL  +P+ 
Sbjct: 12  FLFLSLISL--TLATDYPLFTKSRWIVNN-KGHRVKLACANWPSHLKPVVAEGLSSQPMD 71

Query: 69  DLADEAVRLRFNCVRLTYATHMF---TRFANRTVEENFDLLDLKASKAGLALHNPFVLNM 128
            ++ +   + FNCVRLT+   +    T   N TV+++F+   L     G+  HNP+++N 
Sbjct: 72  SISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVNT 131

Query: 129 TIFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVA 188
            +   ++A V  LG   +MVI DNH + P WCCS DD + FFGD  F+P+ W+ GL+ +A
Sbjct: 132 PLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKMA 191

Query: 189 RRFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDNDLR 248
             F N   VV MSLRNELRG    SKDW KYM +GA  +H  N N+LVI+SGLNFD DL 
Sbjct: 192 TIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADLS 251

Query: 249 CQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVMEGA 308
             +  P+ L +   KLV E+H YSF+  +     HN +N  CS++ +       FV++  
Sbjct: 252 FLKDRPVNL-SFKKKLVLELHWYSFTDGTGQWKSHN-VNDFCSQMFSKERRTGGFVLD-- 311

Query: 309 EAVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKVEPEEV 368
           +  PLF+SEFG DQRG +   +R+++C  A  A+KDLDWA+W+  G YY+R+GK    E 
Sbjct: 312 QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVEA 371

Query: 369 FGVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCVKVKDNM-DKEI 428
           +G+L+ NW  V N  + +   ++Q     P    ++   ++HP +G C+  K +  + E+
Sbjct: 372 YGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESEL 431

Query: 429 YLNNCSNASHWSHEGDGAPIMLEATDLCLKA-NGNGVPPSLSRDCFDEQSGWTAISDSKL 488
            L  C+    WS+   G   +      CL+     G    L R C   +     IS +K+
Sbjct: 432 TLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ----ISATKM 491

Query: 489 HLATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFEL 537
           HL+  T +G+     +CL+ +S N+  +V   C C+  D+ C  +  +QWF++
Sbjct: 492 HLSFNTSDGSL----VCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFKI 525

[Close]

BLAST of Lsi09G005270 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 303.1 bits (775), Expect = 4.4e-82
Identity = 188/533 (35.27%), Postives = 280/533 (52.53%), Query Frame = 0


Query: 10  VFLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKD 69
           +F  FIS  S L+ S PLST  RWIVD   G+RVKL CVNWP+H Q  + EGL ++PL  
Sbjct: 9   LFFLFISSTSKLTTSYPLSTKSRWIVDE-KGQRVKLACVNWPAHLQPTVAEGLSKQPLDS 68

Query: 70  LADEAVRLRFNCVRLTYATHMFTR---FANRTVEENFDLLDLKASKAGLALHNPFVLNMT 129
           ++ + V + FNCVRLT+   + T        TV+++F+ L L     G+  HNP +L++ 
Sbjct: 69  ISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLP 128

Query: 130 IFEAYEAAVDVLGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEGLRLVAR 189
           +F A++  V  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GLR +A 
Sbjct: 129 LFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMAT 188

Query: 190 RFCNKSTVVAMSLRNELRGAKSKSKDWNKYMTQGATTIHNINLNILVIISGLNFDNDLRC 249
            F N + V+ MSLRNE RGA+     W ++M QGA  +H  N  +LVI+SG++FD +L  
Sbjct: 189 LFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSF 248

Query: 250 QRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVERAEFVMEGAE 309
            R   + + +  +KLVFE+H YSFS    S   HN  N  C KII        F++    
Sbjct: 249 LRDRSVNV-SFTDKLVFELHWYSFSDGRDSWRKHNS-NDFCVKIIEKVTHNGGFLL--GR 308

Query: 310 AVPLFVSEFGFDQRGVNEADDRFLSCFNAHLAKKDLDWALWSWQGSYYYRQGKVEPEEVF 369
             PL +SEFG DQRG + + +R+++C  A  A+ DLDWA+W+  G YY R G        
Sbjct: 309 GFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG-------- 368

Query: 370 GVLNYNWTEVRNPNFPQIFQLLQTMLQDPKSNSSNTYVMYHPQSGQCV--KVKDNMDKEI 429
                                             N  +++HP +G CV     DN+   +
Sbjct: 369 -----------------------------PGLRPNKNLLFHPSTGLCVTNNPSDNI-PTL 428

Query: 430 YLNNCSNASHWSHEGDGAPIMLEATDLCLKANGNGVPPSLSRDCFDEQSGWTAISDSKLH 489
            L  C  +  W+        +L    +C++A  N V   +      + S    IS +K+H
Sbjct: 429 RLGPCPKSDPWTFNPSEG--ILWINKMCVEA-PNVVGQKVKLGVGTKCSKLGQISATKMH 488

Query: 490 LATLTKEGNNNNNGLCLEKESSNSTKIVMGRCVCVGNDSNCLQDTQAQWFELV 538
           L+  T    +N   LCL+ +  +++ +V  RC  +  D++C  D  +QWF+++
Sbjct: 489 LSFKT----SNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWFKVL 488

The following BLAST results are available for this feature:

BLAST of Lsi09G005270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	1.0e-115	42.29	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P19487	3.9e-11	24.40	Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra...	[more]

BLAST of Lsi09G005270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UH07	1.6e-286	88.38	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3CTF8	8.1e-286	88.01	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV...	[more]
A0A6J1H201	6.9e-269	82.66	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 P...	[more]
A0A6J1K1F7	4.5e-268	82.10	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=...	[more]
A0A6J1DXU9	2.7e-257	79.93	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 ...	[more]

BLAST of Lsi09G005270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875504.1	7.3e-297	91.50	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
KAA0055112.1	3.4e-286	88.38	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_008467306.1	1.7e-285	88.01	PREDICTED: major extracellular endoglucanase-like [Cucumis melo]	[more]
XP_004143723.1	4.1e-284	87.94	glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical...	[more]
XP_023534098.1	2.9e-269	83.21	uncharacterized protein LOC111795760 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi09G005270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13130.1	2.5e-117	41.00	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26130.1	1.4e-112	40.52	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	7.4e-106	39.96	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	8.2e-97	37.34	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	4.4e-82	35.27	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 66..350 e-value: 1.4E-23 score: 83.8
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..394 e-value: 6.4E-66 score: 224.9
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 8..538
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 400..536 score: 9.627
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 398..522
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..385

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G005270.1	Lsi09G005270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds