Lsi09G004550 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G004550
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptiondisease resistance RPP13-like protein 4
Locationchr09: 4800007 .. 4802160 (+)
RNA-Seq ExpressionLsi09G004550
SyntenyLsi09G004550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCCCTTCTCCGATTCTCGATTCTCAAACTTCAACCTCAATCCCAACAATTCCTCAAATGATCGAGATCCTTTCAAAGTCGCACGAACAGCTCTCCACAGCCCTCCAAAAACATTTCGCCCAGGCCAAATCCACCACTACACCCACTCAAGCTGCCGGCAATGAGATAGTTCCAGCCGTCGAGCCCCCTGCTCCGACAGAAGCCCCTGAAGAACAGCACGGTGAGAAAGAAACAGAACAAGCACAAGAAATTAGTGGCAACATCGAAACCCATAAAGACCTCAAAGTTGGGAAATTGCTGAGCAACATCCAGCTTTTGAAGGAGGCGTTGGAGACGATGATAAATAATCGCGATGAGAAGCTCAGTGTGCCAATACAGATCATAAAAGACAGTCTCGATGTGGTTATCAAAAAGGTCGAAGAAGCTCCTGCTGGTTCTCCACTGACCCAGCAGATCGGAAAGGAATATCTCGACGCCGTAAGCAAGGATATTAGGACGTTGAAGTTCCGGATTCCTTCGTACCGCAAGTTGTCCTTGGCCAAAACCACCGCCCATTTTGGTGACAGGGGAAGTGATACGCGGAAGTCGAATGAGTTCTTCAGGTTGCCTAACTTGTATGGCGATAAGGTGTTCGACAAAAGTTCCGCTTTCATAGAAATTCAGACAGTTTACGATGCGTTGAATGAAAACCTTAAGAAATGCTTTCGGTATTTTGCTGTGTTTCCTGAAAATGTAGTGTTAAAGAAGCGGTTTCTTACCCATTGGTGGATTGGAGAAGGCCTATTGGACGATTTAGGAACTGGAGATTCAAATCCAGAGGATGGGGCCAGTAAGATTCTTCAGATATTTGTAGAGAAGGGTTTGATCGTGCCGGTGAAGGAAGAACAGAAGAAGGTCAAAAAGAAATTTAGAATGCCCCCTCTTGTGCGTTCTGCTGCCATTAATTTGGCCAAAGAAAAGTTTTTAAATTATGATGAAGGGGACAACCCAATTGGTATATCTTGTGACAGGATTTTTCTGGTGAAGGGGGGAGGCTTTTGTCCGCCGAAAGCTCCGACGGAAGAGCGGATGAAGGGTCAGAATTTGGAAGAAAAGATCGGAACAATCTTCAATGTTAGCCAACCTTTCCCTGATCTTGCATTGGAGTGGTTGGCCAAGCAGGGGATTATAGACATGAGAACTACCAAAGTTGTGGAGTGGCTGCTAAAGCTGAAAAACCTCAAAGTTCTTTACTTGGGGAGGTGGCAAAGTGAAGTTGATGAGGAGGAGCATGTGATTGAGGTTGAAAGCCTTGAGTTCTTAGAAGGTTTGAAGAAGATGAAAAAATTGAGGCTTTTGAGCCTTCAGGGGATATTTTGGATCAATAAGCTTCCAAATTCCATAAATAAGCTCGGTGAGCTCAGGGTTTTAGACTTGAAATGTTGTTACAATCTGGAGAAACTGCCTGGAGGTATAGGATCTCTCAAGAGCCTTACACATTTAGATGTCACTGGCTGCTATATGCTCAATGGAATGCCAAAGAGCATATATAAACTCACTCAACTGAGAGTCTTGAAGGGGTTTGTTACAGGAAAGTCAGGTCTTAATTATCTAAAAGGCTTAAAGAAACTGAGAAAGTTGAGTATCAACACAAGCAGCCAGGATTTTCCTAAAGAGACAGATCTACTTGTTCTTCAAGATCTTGGGGAGCACGGTAAGCTTCGAAATCTCTCGATTATGTGGGCAGCCCAAGGGGTGAAGTCGGATGAACCTGATCCCGAGACTTTAAAAGGAAAGATTGTACGAAATCTCTCCAAGAAATTGAACAAAGCAACCGTGCCACCCAATAATAAGACCCCAGATCTGCCAACGAAATTAGAGAAGCTGGAGCTCGAATGTCTATGGAAAGAAGAACTACCCAACTGGCTAAATCCTTTAAACTTGAAAAGCTTGAAGAAACTCTACATTAGAGGGGGGAGCCTAAAAACGCTCGGAGACAAAAAGTGGGAGGCGGCTGAGGTCGTTCGTCTGAAATACATGCAGAAGTTAAAGATAAAATGGGGAAAACTTCAAGAGAATTTTCCAAAGTTGAGTTACTTGCAGAAAGTAGAATGTCCAAAAGTCACTTTCTGCCCATGTGATGCCAATGGAGTTTGGATAAAGCCATGA

mRNA sequence

ATGGCTTCCCCTTCTCCGATTCTCGATTCTCAAACTTCAACCTCAATCCCAACAATTCCTCAAATGATCGAGATCCTTTCAAAGTCGCACGAACAGCTCTCCACAGCCCTCCAAAAACATTTCGCCCAGGCCAAATCCACCACTACACCCACTCAAGCTGCCGGCAATGAGATAGTTCCAGCCGTCGAGCCCCCTGCTCCGACAGAAGCCCCTGAAGAACAGCACGGTGAGAAAGAAACAGAACAAGCACAAGAAATTAGTGGCAACATCGAAACCCATAAAGACCTCAAAGTTGGGAAATTGCTGAGCAACATCCAGCTTTTGAAGGAGGCGTTGGAGACGATGATAAATAATCGCGATGAGAAGCTCAGTGTGCCAATACAGATCATAAAAGACAGTCTCGATGTGGTTATCAAAAAGGTCGAAGAAGCTCCTGCTGGTTCTCCACTGACCCAGCAGATCGGAAAGGAATATCTCGACGCCGTAAGCAAGGATATTAGGACGTTGAAGTTCCGGATTCCTTCGTACCGCAAGTTGTCCTTGGCCAAAACCACCGCCCATTTTGGTGACAGGGGAAGTGATACGCGGAAGTCGAATGAGTTCTTCAGGTTGCCTAACTTGTATGGCGATAAGGTGTTCGACAAAAGTTCCGCTTTCATAGAAATTCAGACAGTTTACGATGCGTTGAATGAAAACCTTAAGAAATGCTTTCGGTATTTTGCTGTGTTTCCTGAAAATGTAGTGTTAAAGAAGCGGTTTCTTACCCATTGGTGGATTGGAGAAGGCCTATTGGACGATTTAGGAACTGGAGATTCAAATCCAGAGGATGGGGCCAGTAAGATTCTTCAGATATTTGTAGAGAAGGGTTTGATCGTGCCGGTGAAGGAAGAACAGAAGAAGGTCAAAAAGAAATTTAGAATGCCCCCTCTTGTGCGTTCTGCTGCCATTAATTTGGCCAAAGAAAAGTTTTTAAATTATGATGAAGGGGACAACCCAATTGGTATATCTTGTGACAGGATTTTTCTGGTGAAGGGGGGAGGCTTTTGTCCGCCGAAAGCTCCGACGGAAGAGCGGATGAAGGGTCAGAATTTGGAAGAAAAGATCGGAACAATCTTCAATGTTAGCCAACCTTTCCCTGATCTTGCATTGGAGTGGTTGGCCAAGCAGGGGATTATAGACATGAGAACTACCAAAGTTGTGGAGTGGCTGCTAAAGCTGAAAAACCTCAAAGTTCTTTACTTGGGGAGGTGGCAAAGTGAAGTTGATGAGGAGGAGCATGTGATTGAGGTTGAAAGCCTTGAGTTCTTAGAAGGTTTGAAGAAGATGAAAAAATTGAGGCTTTTGAGCCTTCAGGGGATATTTTGGATCAATAAGCTTCCAAATTCCATAAATAAGCTCGGTGAGCTCAGGGTTTTAGACTTGAAATGTTGTTACAATCTGGAGAAACTGCCTGGAGGTATAGGATCTCTCAAGAGCCTTACACATTTAGATGTCACTGGCTGCTATATGCTCAATGGAATGCCAAAGAGCATATATAAACTCACTCAACTGAGAGTCTTGAAGGGGTTTGTTACAGGAAAGTCAGGTCTTAATTATCTAAAAGGCTTAAAGAAACTGAGAAAGTTGAGTATCAACACAAGCAGCCAGGATTTTCCTAAAGAGACAGATCTACTTGTTCTTCAAGATCTTGGGGAGCACGGTAAGCTTCGAAATCTCTCGATTATGTGGGCAGCCCAAGGGGTGAAGTCGGATGAACCTGATCCCGAGACTTTAAAAGGAAAGATTGTACGAAATCTCTCCAAGAAATTGAACAAAGCAACCGTGCCACCCAATAATAAGACCCCAGATCTGCCAACGAAATTAGAGAAGCTGGAGCTCGAATGTCTATGGAAAGAAGAACTACCCAACTGGCTAAATCCTTTAAACTTGAAAAGCTTGAAGAAACTCTACATTAGAGGGGGGAGCCTAAAAACGCTCGGAGACAAAAAGTGGGAGGCGGCTGAGGTCGTTCGTCTGAAATACATGCAGAAGTTAAAGATAAAATGGGGAAAACTTCAAGAGAATTTTCCAAAGTTGAGTTACTTGCAGAAAGTAGAATGTCCAAAAGTCACTTTCTGCCCATGTGATGCCAATGGAGTTTGGATAAAGCCATGA

Coding sequence (CDS)

ATGGCTTCCCCTTCTCCGATTCTCGATTCTCAAACTTCAACCTCAATCCCAACAATTCCTCAAATGATCGAGATCCTTTCAAAGTCGCACGAACAGCTCTCCACAGCCCTCCAAAAACATTTCGCCCAGGCCAAATCCACCACTACACCCACTCAAGCTGCCGGCAATGAGATAGTTCCAGCCGTCGAGCCCCCTGCTCCGACAGAAGCCCCTGAAGAACAGCACGGTGAGAAAGAAACAGAACAAGCACAAGAAATTAGTGGCAACATCGAAACCCATAAAGACCTCAAAGTTGGGAAATTGCTGAGCAACATCCAGCTTTTGAAGGAGGCGTTGGAGACGATGATAAATAATCGCGATGAGAAGCTCAGTGTGCCAATACAGATCATAAAAGACAGTCTCGATGTGGTTATCAAAAAGGTCGAAGAAGCTCCTGCTGGTTCTCCACTGACCCAGCAGATCGGAAAGGAATATCTCGACGCCGTAAGCAAGGATATTAGGACGTTGAAGTTCCGGATTCCTTCGTACCGCAAGTTGTCCTTGGCCAAAACCACCGCCCATTTTGGTGACAGGGGAAGTGATACGCGGAAGTCGAATGAGTTCTTCAGGTTGCCTAACTTGTATGGCGATAAGGTGTTCGACAAAAGTTCCGCTTTCATAGAAATTCAGACAGTTTACGATGCGTTGAATGAAAACCTTAAGAAATGCTTTCGGTATTTTGCTGTGTTTCCTGAAAATGTAGTGTTAAAGAAGCGGTTTCTTACCCATTGGTGGATTGGAGAAGGCCTATTGGACGATTTAGGAACTGGAGATTCAAATCCAGAGGATGGGGCCAGTAAGATTCTTCAGATATTTGTAGAGAAGGGTTTGATCGTGCCGGTGAAGGAAGAACAGAAGAAGGTCAAAAAGAAATTTAGAATGCCCCCTCTTGTGCGTTCTGCTGCCATTAATTTGGCCAAAGAAAAGTTTTTAAATTATGATGAAGGGGACAACCCAATTGGTATATCTTGTGACAGGATTTTTCTGGTGAAGGGGGGAGGCTTTTGTCCGCCGAAAGCTCCGACGGAAGAGCGGATGAAGGGTCAGAATTTGGAAGAAAAGATCGGAACAATCTTCAATGTTAGCCAACCTTTCCCTGATCTTGCATTGGAGTGGTTGGCCAAGCAGGGGATTATAGACATGAGAACTACCAAAGTTGTGGAGTGGCTGCTAAAGCTGAAAAACCTCAAAGTTCTTTACTTGGGGAGGTGGCAAAGTGAAGTTGATGAGGAGGAGCATGTGATTGAGGTTGAAAGCCTTGAGTTCTTAGAAGGTTTGAAGAAGATGAAAAAATTGAGGCTTTTGAGCCTTCAGGGGATATTTTGGATCAATAAGCTTCCAAATTCCATAAATAAGCTCGGTGAGCTCAGGGTTTTAGACTTGAAATGTTGTTACAATCTGGAGAAACTGCCTGGAGGTATAGGATCTCTCAAGAGCCTTACACATTTAGATGTCACTGGCTGCTATATGCTCAATGGAATGCCAAAGAGCATATATAAACTCACTCAACTGAGAGTCTTGAAGGGGTTTGTTACAGGAAAGTCAGGTCTTAATTATCTAAAAGGCTTAAAGAAACTGAGAAAGTTGAGTATCAACACAAGCAGCCAGGATTTTCCTAAAGAGACAGATCTACTTGTTCTTCAAGATCTTGGGGAGCACGGTAAGCTTCGAAATCTCTCGATTATGTGGGCAGCCCAAGGGGTGAAGTCGGATGAACCTGATCCCGAGACTTTAAAAGGAAAGATTGTACGAAATCTCTCCAAGAAATTGAACAAAGCAACCGTGCCACCCAATAATAAGACCCCAGATCTGCCAACGAAATTAGAGAAGCTGGAGCTCGAATGTCTATGGAAAGAAGAACTACCCAACTGGCTAAATCCTTTAAACTTGAAAAGCTTGAAGAAACTCTACATTAGAGGGGGGAGCCTAAAAACGCTCGGAGACAAAAAGTGGGAGGCGGCTGAGGTCGTTCGTCTGAAATACATGCAGAAGTTAAAGATAAAATGGGGAAAACTTCAAGAGAATTTTCCAAAGTTGAGTTACTTGCAGAAAGTAGAATGTCCAAAAGTCACTTTCTGCCCATGTGATGCCAATGGAGTTTGGATAAAGCCATGA

Protein sequence

MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVPAVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRDEKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLSLAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEKFLNYDEGDNPIGISCDRIFLVKGGGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSLKTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIKP
Homology
BLAST of Lsi09G004550 vs. ExPASy Swiss-Prot
Match: Q38834 (Disease resistance RPP13-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RPP13L4 PE=1 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 5.2e-27
Identity = 150/625 (24.00%), Postives = 256/625 (40.96%), Query Frame = 0

Query: 110 EALETMINNRDEKLSVPIQIIKDSLDVVIKKV----EEAPAGSPLTQQIGKEYLDAVSKD 169
           E++   +  RD+K   P  +  D+  ++   V     +     P  + +GKE    +   
Sbjct: 299 ESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKE----IVTK 358

Query: 170 IRTLKFRIPSYRKLSLAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTV 229
            + L   I +   L L K   +      + R+  E F+   L G+   +  +    +Q  
Sbjct: 359 CKGLPLTIKAVGGLLLCKDHVY-----HEWRRIAEHFQ-DELRGN-TSETDNVMSSLQLS 418

Query: 230 YDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIF 289
           YD L  +LK C    +++PE+ V+ K+ L H WIGEG +  +     +  +         
Sbjct: 419 YDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFV--MWRNGRSATESGEDCFSGL 478

Query: 290 VEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEKFLNYDEGDNPIGISCDRIFLVKG 349
             + LI  V +         ++  +VR   I++AK+     D   NP G++C  + +   
Sbjct: 479 TNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKK-----DSFSNPEGLNCRHLGI--S 538

Query: 350 GGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLK 409
           G F   +     +++G     K G +  ++    DLA ++                    
Sbjct: 539 GNFDEKQIKVNHKLRGVVSTTKTGEVNKLNS---DLAKKF------------------TD 598

Query: 410 LKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINK 469
            K L+VL         D  + + +    E L+ +  ++ L  LSL     + + P S+  
Sbjct: 599 CKYLRVL---------DISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMED 658

Query: 470 LGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVT 529
           L  L++LD   C NL++L   I   K L  LD+T C  L   PK I  L +L VL GF  
Sbjct: 659 LHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKP 718

Query: 530 GKSG----LNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGV 589
            +S     L+ +K L  LRKL ++ +  D  +E +L  L +L    KL ++SI       
Sbjct: 719 ARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINL---SKLMSISI------- 778

Query: 590 KSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPL 649
              +   + L  KI          A  PP+        +L +L L+    +  P+WL+P 
Sbjct: 779 NCYDSYGDDLITKI---------DALTPPH--------QLHELSLQFYPGKSSPSWLSPH 838

Query: 650 NLKSLKKLYIRGGSLKTLGDKKWE------AAEVVRLKYMQKLKIKWGKLQENFPKLSYL 709
            L  L+ + I  G+L  + +  W         E + L  +  L + W  LQ++ P L  +
Sbjct: 839 KLPMLRYMSICSGNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDWEVLQQSMPYLRTV 846

Query: 710 QKVECPKV-TFCPCDA---NGVWIK 717
               CP++ +F   D     GVW+K
Sbjct: 899 TANWCPELESFAIEDVGFRGGVWMK 846

BLAST of Lsi09G004550 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 4.6e-15
Identity = 113/443 (25.51%), Postives = 168/443 (37.92%), Query Frame = 0

Query: 214 DKSSAFIEIQTVYDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSN 273
           D+SS    ++  Y  L  +L++CF Y AVFP++  + K  L  +W+  G L  L  G+  
Sbjct: 383 DESSILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFL--LSKGNLE 442

Query: 274 PEDGASKILQIFVEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINL--AKEKFLNYDEGDN 333
            ED  +++      +     ++ E  K    F+M  L+   A +L  A     N  E   
Sbjct: 443 LEDVGNEVWNELYLRSFFQEIEVESGKT--YFKMHDLIHDLATSLFSANTSSSNIRE--- 502

Query: 334 PIGISCDRIFLVKGGGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGI 393
            I  + D   +                         IG    VS   P L  ++++    
Sbjct: 503 -INANYDGYMM------------------------SIGFAEVVSSYSPSLLQKFVS---- 562

Query: 394 IDMRTTKVVEWLLKLKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQ 453
                       L++ NL+   L +  S + +  H                  LR L L 
Sbjct: 563 ------------LRVLNLRNSNLNQLPSSIGDLVH------------------LRYLDLS 622

Query: 454 GIFWINKLPNSINKLGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI 513
           G F I  LP  + KL  L+ LDL  C +L  LP     L SL +L + GC  L   P  I
Sbjct: 623 GNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGC-SLTSTPPRI 682

Query: 514 YKLTQLRVLKGFVTGKSGLNYLKGLKKLRKL-SINTSSQDFPKETDLLVLQDLGEHGKLR 573
             LT L+ L  FV GK   + L  LK L    SI+ +  D  K+       +L     L 
Sbjct: 683 GLLTCLKSLSCFVIGKRKGHQLGELKNLNLYGSISITKLDRVKKDTDAKEANLSAKANLH 735

Query: 574 NLSIMWAAQGVKSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLW 633
           +L + W   G      D E L             +A  P +N        L+ LE+    
Sbjct: 743 SLCLSWDLDG--KHRYDSEVL-------------EALKPHSN--------LKYLEINGFG 735

Query: 634 KEELPNWLNPLNLKSLKKLYIRG 654
              LP+W+N   LK++  + IRG
Sbjct: 803 GIRLPDWMNQSVLKNVVSIRIRG 735

BLAST of Lsi09G004550 vs. ExPASy Swiss-Prot
Match: Q9LVT4 (Probable disease resistance protein At5g47250 OS=Arabidopsis thaliana OX=3702 GN=At5g47250 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.2e-11
Identity = 120/515 (23.30%), Postives = 213/515 (41.36%), Query Frame = 0

Query: 219 FIEIQTVYDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGA 278
           F  ++  YD L     KCF Y A+FP+   +K+  L  +WIGEG +D+   G    +D  
Sbjct: 390 FQVLKLSYDYLKTKNAKCFLYCALFPKAYYIKQDELVEYWIGEGFIDE-KDGRERAKDRG 449

Query: 279 SKILQIFVEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEKFLNYDEGDNPIGISCD 338
            +I+   V  GL++       +  KK  M  ++R  A+ +  E    + +G+        
Sbjct: 450 YEIIDNLVGAGLLL-------ESNKKVYMHDMIRDMALWIVSE----FRDGER------- 509

Query: 339 RIFLVKGGGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEW--LAKQGIIDMRT 398
             ++VK                         T   +SQ  PD+  +W  + K  + +   
Sbjct: 510 --YVVK-------------------------TDAGLSQ-LPDVT-DWTTVTKMSLFNNEI 569

Query: 399 TKVVE--WLLKLKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIF 458
             + +        NL  L+L        +   ++++    FL     M  L +L L   F
Sbjct: 570 KNIPDDPEFPDQTNLVTLFL--------QNNRLVDIVGKFFL----VMSTLVVLDLSWNF 629

Query: 459 WINKLPNSINKLGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKL 518
            I +LP  I+ L  LR+L+L    +++ LP G+G L  L HL++     L  +   I +L
Sbjct: 630 QITELPKGISALVSLRLLNLSGT-SIKHLPEGLGVLSKLIHLNLESTSNLRSV-GLISEL 689

Query: 519 TQLRVLKGFVTGKS----GLNYLKGLKKLRKLSINTSSQDFPKE-TDLLVLQDLGEHGKL 578
            +L+VL+ + +  +     L  L+ LK L+ L++  ++    +E      L  + +   L
Sbjct: 690 QKLQVLRFYGSAAALDCCLLKILEQLKGLQLLTVTVNNDSVLEEFLGSTRLAGMTQGIYL 749

Query: 579 RNLSIMWAAQGVKSDEPDPETL-----------KGKIVRNLSKKLNKATVPPNNKTPDLP 638
             L + +AA G  S     E +           +GK     S   + + + P+N  P   
Sbjct: 750 EGLKVSFAAIGTLSSLHKLEMVNCDITESGTEWEGKRRDQYSPSTSSSEITPSN--PWFK 809

Query: 639 TKLEKLELECLWKEELPNWLNPLNLKSLK-------KLYIRGGSLKTLGDKKWEAAEVVR 698
                +   C+  ++L   +   NL+SL           I     + +G   ++  +V+R
Sbjct: 810 DLSAVVINSCIHLKDLTWLMYAANLESLSVESSPKMTELINKEKAQGVGVDPFQELQVLR 838

Query: 699 LKYMQKLKIKWGKLQENFPKLSYLQKVE---CPKV 704
           L Y+++L   +G  Q +FPKL  L KV+   CP +
Sbjct: 870 LHYLKELGSIYGS-QVSFPKLK-LNKVDIENCPNL 838

BLAST of Lsi09G004550 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 2.0e-10
Identity = 122/537 (22.72%), Postives = 210/537 (39.11%), Query Frame = 0

Query: 197 KSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENLKKCFRYFAVFPENVVLKKRFLTH 256
           + +E + LP        D++S    ++  Y  L  +L++CF Y AVFP++  ++K +L  
Sbjct: 373 RDSEIWNLPQ-------DENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIA 432

Query: 257 WWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQKKVKKKFRMPPLVRSAAI 316
            W+    L  L  G+   ED  +++      +     +  E K  K  F+M  L+   A 
Sbjct: 433 LWMAHSFL--LSKGNMELEDVGNEVWNELYLRSFFQEI--EVKSGKTYFKMHDLIHDLAT 492

Query: 317 NLAKEKFLNYDEGDNPIGISCDRIFLVKGGGFCPPKAPTEERMKGQNLEEKIGTIFN--V 376
           ++      +       +    D +F+V                   N ++ +   F+  V
Sbjct: 493 SMFSASASSRSIRQINVKDDEDMMFIV------------------TNYKDMMSIGFSEVV 552

Query: 377 SQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGRWQSEVDEEEHVIEVESLE 436
           S   P L   +++                L++ NL      +  S V +  H        
Sbjct: 553 SSYSPSLFKRFVS----------------LRVLNLSNSEFEQLPSSVGDLVH-------- 612

Query: 437 FLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKCCYNLEKLPGGIGSLKSLT 496
                     LR L L G   I  LP  + KL  L+ LDL  C +L  LP     L SL 
Sbjct: 613 ----------LRYLDLSG-NKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLR 672

Query: 497 HLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKS-----------------GLNYLKGLK 556
           +L +  C  L  MP  I  LT L+ L  FV G+                   + +L+ +K
Sbjct: 673 NLVLDHC-PLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNLNLRGAISITHLERVK 732

Query: 557 --------------KLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKS 616
                          L  LS++    +  +  ++ VL+ L  H  L+ L I+        
Sbjct: 733 NDMEAKEANLSAKANLHSLSMSWDRPNRYESEEVKVLEALKPHPNLKYLEIIDFCGFCLP 792

Query: 617 DEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPT-KLEKLELECLWKEELPNWLNPLN 676
           D  +   LK  +   +S   N + +PP  + P L + +L+   +E  + E+   +L    
Sbjct: 793 DWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLELQDGSVEVEYVED-SGFLTRRR 839

Query: 677 LKSLKKLYIRGGSLKTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVE 700
             SL+KL+I GG     G ++ + AE  +   ++++KI    +   FP LS ++K+E
Sbjct: 853 FPSLRKLHI-GGFCNLKGLQRMKGAE--QFPVLEEMKISDCPMFV-FPTLSSVKKLE 839

BLAST of Lsi09G004550 vs. ExPASy Swiss-Prot
Match: Q8W3K3 (Putative disease resistance protein At1g58400 OS=Arabidopsis thaliana OX=3702 GN=At1g58400 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.4e-10
Identity = 98/437 (22.43%), Postives = 177/437 (40.50%), Query Frame = 0

Query: 214 DKSSAFIEIQTVYDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSN 273
           + SS +  +   ++ L   LK CF Y A FPE+  +K   L++ W  EG+L+        
Sbjct: 405 NNSSVYHVLSLSFEELPSYLKHCFLYLAHFPEDHNIKVEKLSYCWAAEGILEPRHYHGQT 464

Query: 274 PEDGASKILQIFVEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEKFLNYDEGDNPI 333
             D     ++  V + +++  ++      +   +  ++R   +  AKE+  N+       
Sbjct: 465 IRDVGESYIEELVRRNMVIAERDVTTLRFEACHLHDMMREVCLLKAKEE--NF------- 524

Query: 334 GISCDRIFLVKGGGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIID 393
                    V+     PP A ++     +    +  T  +VS+   +  L+ L       
Sbjct: 525 ---------VQIASILPPTANSQYPGTSRRFVSQNPTTLHVSRDINNPKLQSLLIVWENR 584

Query: 394 MRTTKVV-EWLLKLKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQG 453
            ++ K++    ++L+ L+VL L           +  + E      G+ K+  LR L+L  
Sbjct: 585 RKSWKLLGSSFIRLELLRVLDL-----------YKAKFEGRNLPSGIGKLIHLRYLNLD- 644

Query: 454 IFWINKLPNSINKLGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIY 513
           +  +++LP+S+  L  L  LD+  C     +P  +  +  L +L +        +   + 
Sbjct: 645 LARVSRLPSSLGNLRLLIYLDINVCTKSLFVPNCLMGMHELRYLRLP-FNTSKEIKLGLC 704

Query: 514 KLTQLRVLKGFVTGKSGLNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNL 573
            L  L  L+ F T  S L  L+G+  LR L+I    +   KET  L    LG    L NL
Sbjct: 705 NLVNLETLENFSTENSSLEDLRGMVSLRTLTIGL-FKHISKET--LFASILGMR-HLENL 764

Query: 574 SIMW--AAQGVKSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLW 633
           SI     +   K    D   L    ++ L+ +L    +P     P   T +  L+  CL 
Sbjct: 765 SIRTPDGSSKFKRIMEDGIVLDAIHLKQLNLRLYMPKLPDEQHFPSHLTSI-SLDGCCLV 805

Query: 634 KEELPNWLNPLNLKSLK 648
           ++ LP     L LK ++
Sbjct: 825 EDPLPILEKLLELKEVR 805

BLAST of Lsi09G004550 vs. ExPASy TrEMBL
Match: A0A5A7TJK3 (Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00340 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.8e-278
Identity = 513/721 (71.15%), Postives = 581/721 (80.58%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPS IL  Q ST IPTIPQMIE LS  +++LSTALQKH  QA                
Sbjct: 1   MASPSLILHPQNSTPIPTIPQMIETLSDLYKKLSTALQKHSTQA---------------- 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
             EP             K+T+Q  EISG+I+ +KD KV KL SNI LL EALE MINNRD
Sbjct: 61  --EP------------NKQTQQIPEISGSIDANKDPKVVKLQSNIMLLMEALEMMINNRD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           +KL+VPI+ I+ +LD+VIK+V +AP GSPLTQ+IG++YLDAV KDIR LKFRIPSYRKLS
Sbjct: 121 KKLNVPIRSIETNLDIVIKRVNDAPPGSPLTQKIGEDYLDAVIKDIRMLKFRIPSYRKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LAKT AH G RGS      EF  LPNL+GDKVFD+SSAF E+Q +Y   ++++ +KCF Y
Sbjct: 181 LAKTVAHSGGRGSHALTPIEFV-LPNLHGDKVFDESSAFKEVQKIYYEFSDDIFRKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKKR LT+WWIGEG+LD  GTGDSNPED A +IL  FVEKGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKRSLTYWWIGEGILDVSGTGDSNPEDKAGEILLKFVEKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLAKE-KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVRSA+I LA E KFLN+D+  NP      CD+I LVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRSASIKLAIENKFLNFDDWGNPTYRSFGCDKILLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K QNLE+ + TIFNVSQPFPD ALEWLAK+G +DMRTTKVVEWLLKL +LKVLYLGR
Sbjct: 361 ---KYQNLEKDLVTIFNVSQPFPDSALEWLAKKGDVDMRTTKVVEWLLKLDHLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHVIEV SLEFLEGLKKMK LRLLSLQGIFWIN+LPNSIN L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVIEVLSLEFLEGLKKMKTLRLLSLQGIFWINELPNSINMLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGFVTGKS LN LKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFVTGKSSLNDLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINT+S  FP   DL VL++LGEHG+LRNLSIMWAA+G+K D+P  +T KG  +
Sbjct: 541 KKLRKLSINTNSPGFPDAKDLRVLRELGEHGELRNLSIMWAAEGLKFDQPPSKTEKGTFI 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           R L+K+++K T  PN++T +LP  LEKLELECL ++ LPNWLNP NL SLKKLYIRGGSL
Sbjct: 601 RELAKQISKLTAQPNDETSELPKNLEKLELECLREKNLPNWLNPDNLTSLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 718
           +TLG+KKWEAAEVVRLKY+ KLKIKW +LQ NFPKLSYLQKV+CP VT  PCDANGVWIK
Sbjct: 661 ETLGNKKWEAAEVVRLKYITKLKIKWRELQNNFPKLSYLQKVKCPSVTLFPCDANGVWIK 686

BLAST of Lsi09G004550 vs. ExPASy TrEMBL
Match: A0A1S4E678 (uncharacterized protein LOC103482581 OS=Cucumis melo OX=3656 GN=LOC103482581 PE=4 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.8e-277
Identity = 512/720 (71.11%), Postives = 580/720 (80.56%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPS IL  Q ST IPTIPQMIE LS  +++LSTALQKH  QA                
Sbjct: 1   MASPSLILHPQNSTPIPTIPQMIETLSDLYKKLSTALQKHSTQA---------------- 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
             EP             K+T+Q  EISG+I+ +KD KV KL SNI LL EALE MINNRD
Sbjct: 61  --EP------------NKQTQQIPEISGSIDANKDPKVVKLQSNIMLLMEALEMMINNRD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           +KL+VPI+ I+ +LD+VIK+V +AP GSPLTQ+IG++YLDAV KDIR LKFRIPSYRKLS
Sbjct: 121 KKLNVPIRSIETNLDIVIKRVNDAPPGSPLTQKIGEDYLDAVIKDIRMLKFRIPSYRKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LAKT AH G RGS      EF  LPNL+GDKVFD+SSAF E+Q +Y   ++++ +KCF Y
Sbjct: 181 LAKTVAHSGGRGSHALTPIEFV-LPNLHGDKVFDESSAFKEVQKIYYEFSDDIFRKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKKR LT+WWIGEG+LD  GTGDSNPED A +IL  FVEKGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKRSLTYWWIGEGILDVSGTGDSNPEDKAGEILLKFVEKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLAKE-KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVRSA+I LA E KFLN+D+  NP      CD+I LVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRSASIKLAIENKFLNFDDWGNPTYRSFGCDKILLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K QNLE+ + TIFNVSQPFPD ALEWLAK+G +DMRTTKVVEWLLKL +LKVLYLGR
Sbjct: 361 ---KYQNLEKDLVTIFNVSQPFPDSALEWLAKKGDVDMRTTKVVEWLLKLDHLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHVIEV SLEFLEGLKKMK LRLLSLQGIFWIN+LPNSIN L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVIEVLSLEFLEGLKKMKTLRLLSLQGIFWINELPNSINMLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGFVTGKS LN LKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFVTGKSSLNDLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINT+S  FP   DL VL++LGEHG+LRNLSIMWAA+G+K D+P  +T KG  +
Sbjct: 541 KKLRKLSINTNSPGFPDAKDLRVLRELGEHGELRNLSIMWAAEGLKFDQPPSKTEKGTFI 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           R L+K+++K T  PN++T +LP  LEKLELECL ++ LPNWLNP NL SLKKLYIRGGSL
Sbjct: 601 RELAKQISKLTAQPNDETSELPKNLEKLELECLREKNLPNWLNPDNLTSLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 717
           +TLG+KKWEAAEVVRLKY+ KLKIKW +LQ NFPKLSYLQKV+CP VT  PCDANGVWIK
Sbjct: 661 ETLGNKKWEAAEVVRLKYITKLKIKWRELQNNFPKLSYLQKVKCPSVTLFPCDANGVWIK 685

BLAST of Lsi09G004550 vs. ExPASy TrEMBL
Match: A0A0A0KRD7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G165320 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 7.0e-277
Identity = 511/721 (70.87%), Postives = 579/721 (80.31%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPS IL  Q ST IPTIPQMIE LS  +++LSTALQKH  QA                
Sbjct: 1   MASPSLILHPQNSTPIPTIPQMIETLSDLYKKLSTALQKHTTQA---------------- 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
             EP             K T+Q   ISG I+  KD KV KL SNI LL EALETMINNRD
Sbjct: 61  --EP------------NKPTQQIPGISGTIDNDKDPKVLKLQSNIMLLMEALETMINNRD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           +KL+VPIQ I+ +LD+VIK+V +AP GSPLTQ+IG++YLDA+ +D+RTLKFRIPSYRKLS
Sbjct: 121 KKLNVPIQSIETNLDIVIKRVNDAPPGSPLTQKIGEDYLDAIIQDVRTLKFRIPSYRKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LAKT AH G RGS      EF  LPNL GD+VFD+S AF E+Q +Y   N+++ +KCF Y
Sbjct: 181 LAKTVAHSGGRGSHALTPIEFV-LPNLQGDEVFDESPAFKEVQKIYYEFNDDIFRKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKK+FLT+WWIGEG+LD  GTGDSN ED A  IL  FVEKGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKQFLTYWWIGEGILDVKGTGDSNLEDEAGGILLKFVEKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLAKE-KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVR A+I LA E KFLN+D+  NP      CDRI LVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRFASIKLAIENKFLNFDDWGNPTYRSFGCDRILLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K QNLEEK+ TIFNVSQPFPD ALEWLAK+G +DMRTTKVVEWLLKL++LKVLYLGR
Sbjct: 361 ---KYQNLEEKMVTIFNVSQPFPDSALEWLAKKGDVDMRTTKVVEWLLKLEHLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHVIEV SLEFLEGL+KMKKLRLLSLQGIFWIN+LPNSIN L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVIEVLSLEFLEGLRKMKKLRLLSLQGIFWINELPNSINMLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGFVTGKS LN LKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFVTGKSSLNDLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINT+S  FP   DL VL++LGEHG+LRNLSIMWAA+G+K D+P  +T KG  +
Sbjct: 541 KKLRKLSINTNSPGFPDAKDLRVLRELGEHGELRNLSIMWAAEGLKFDQPPSKTEKGTFI 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           R L+K+++K T  PN++T +LP  LEKLELECL ++ LPNWLNP NL SLKKLYIRGGSL
Sbjct: 601 RELTKQISKLTAQPNDETSELPKNLEKLELECLREKNLPNWLNPDNLTSLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 718
           +TLG+KKWEAAEVVRLKYM +LKIKW +LQ NFPKLSYLQKV+CP+VT  PCDA+GVWIK
Sbjct: 661 ETLGNKKWEAAEVVRLKYMTELKIKWRELQNNFPKLSYLQKVKCPRVTLFPCDASGVWIK 686

BLAST of Lsi09G004550 vs. ExPASy TrEMBL
Match: A0A6J1K7N6 (disease resistance RPP13-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111491378 PE=4 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 5.1e-235
Identity = 460/725 (63.45%), Postives = 540/725 (74.48%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MAS SP L  Q S  IPTIPQMIE LSK H +LSTAL       K +TT  QAA N   P
Sbjct: 1   MASRSPPLHPQASALIPTIPQMIESLSKLHGELSTAL-------KESTTRAQAAANRRAP 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
           A        + ++Q  E E     E + +I  +++ +  KLLSNI+ LK ALE   N  D
Sbjct: 61  A--------SADDQIEEAERPNT-ETNASIRANQNPQFDKLLSNIEHLKGALEA-TNELD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           ++L  PIQ IK SL+ VIK +E       L Q+I + ++DAVSKD++TLKF+IPSY+KLS
Sbjct: 121 KQLKEPIQNIKASLEEVIKSIE-------LDQKIEENFIDAVSKDMKTLKFQIPSYQKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LA +TAH  D+G  T + +E F+LPNLY  +VF++S AFIEIQ +Y+   + + KKCF Y
Sbjct: 181 LAGSTAHLVDKGGGTARQDE-FKLPNLYAHEVFNESPAFIEIQEIYNGFGDVICKKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FA FPENVVLKKR LTH WIGEGLLD  G+GD  PE  A +IL+ F +KGLIVP+KEEQK
Sbjct: 241 FAAFPENVVLKKRLLTHCWIGEGLLDSSGSGDQMPEVRAGEILKKFADKGLIVPLKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLA-KEKFLNYDEGDNPIGIS--CDRIFLVKGGGFCPPKAPTE 360
           KVKKKF MPP+VRSAAI LA KE+FLNYD GDNPIG S  CDRIFLVKGGG   PKAPT 
Sbjct: 301 KVKKKFTMPPIVRSAAIILAKKEQFLNYDSGDNPIGKSPGCDRIFLVKGGGLRQPKAPTV 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
           +    QNLE  + +IFNVSQPFPD ALEWLAK+G +DMRT KVVEWLLKLKNLKVLYLGR
Sbjct: 361 D----QNLEVTVESIFNVSQPFPDSALEWLAKKGEVDMRTDKVVEWLLKLKNLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQS VDE++  IEVESLEFLEGLKKMKKLRLLSLQGI WI KLPNSI  L ELRVLDLK 
Sbjct: 421 WQSAVDEQQ--IEVESLEFLEGLKKMKKLRLLSLQGISWIYKLPNSIRTLSELRVLDLKS 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           C+NLE LP GIGSLK LTHLDV+GCYML+ MPKSI KLT+LRVLKGFVTG+S LN LKG 
Sbjct: 481 CFNLETLPQGIGSLKRLTHLDVSGCYMLDQMPKSISKLTELRVLKGFVTGRSSLNDLKGF 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETL----K 600
           KKLRKLSINT+ +DFP +TDL VLQDLGEHGKLR L I+W A+G+K        +     
Sbjct: 541 KKLRKLSINTNRRDFPNDTDLRVLQDLGEHGKLRTLGIVWVAEGLKQPSSGERNMIRQVT 600

Query: 601 GKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIR 660
            KI R L+ +L+K     N+   +LP +LEKLE+ECL KE+LP WLNP NL SLK+LYIR
Sbjct: 601 RKIGRQLTMQLSKQRDQSNDVILELPKELEKLEMECLPKEKLPLWLNPSNLTSLKRLYIR 660

Query: 661 GGSLKTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANG 718
           GG L  LG + W+ AEVVRLKYM +LKI+W +LQ++FP LSYLQKV CP+VTFCPCDANG
Sbjct: 661 GGKLAELGSEPWK-AEVVRLKYMTELKIEWRELQKSFPNLSYLQKVRCPRVTFCPCDANG 693

BLAST of Lsi09G004550 vs. ExPASy TrEMBL
Match: A0A6J1H2K4 (probable disease resistance protein At1g12280 OS=Cucurbita moschata OX=3662 GN=LOC111459521 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 6.6e-235
Identity = 460/725 (63.45%), Postives = 536/725 (73.93%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MAS S  L  Q S  IPTIPQMIE LSK H +LSTAL       K +TT  QAA N   P
Sbjct: 1   MASRSSPLHPQASDLIPTIPQMIESLSKLHGELSTAL-------KESTTRAQAAANRRAP 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
           A        + ++Q  E E     E + +I  +++ +  KLL+NI+ LK ALE   N  D
Sbjct: 61  ASANRRAPASADDQIEEAERPHT-ETNASIRANQNPQFEKLLTNIEHLKGALEA-TNELD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           ++L  PIQ IK SL  VIK +E       L Q+I + +LDAVSKD++TLKF+IPSY+KLS
Sbjct: 121 KQLKEPIQSIKASLKEVIKSIE-------LDQKIEENFLDAVSKDMKTLKFQIPSYQKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LA  TAH  D+G  T + +E F+LPNLY  KVFD+S AFIEIQ +Y+   +++ KKCF Y
Sbjct: 181 LAGNTAHLVDKGGGTARQDE-FKLPNLYAHKVFDESPAFIEIQDIYNGFGDDICKKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FA FPENVVLKKR LTHWWIGEGLLD  G+GD  PE  A +IL+ F EKGLIVP+KEEQK
Sbjct: 241 FAAFPENVVLKKRLLTHWWIGEGLLDSSGSGDQMPEVRAGEILKKFAEKGLIVPLKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLA-KEKFLNYDEGDNPIGIS--CDRIFLVKGGGFCPPKAPTE 360
           KVKKKF MPP+VRSAAI LA KE+FLNYD GDNPIG S  CDRIFLVKGGG   PKAPT 
Sbjct: 301 KVKKKFTMPPIVRSAAIVLAKKEQFLNYDSGDNPIGKSSGCDRIFLVKGGGLHQPKAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
                QNLE  + +IFNVSQPFPD ALEWLAK+G +DMRT KVVEWLLKLKNLKVLYLGR
Sbjct: 361 -LTTDQNLEVTMESIFNVSQPFPDSALEWLAKKGEVDMRTDKVVEWLLKLKNLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQS VDE++  IEVESLEFLEGLKKMKKLRLLSLQGI WINKLPNSI  L ELRVLDLK 
Sbjct: 421 WQSAVDEQQ--IEVESLEFLEGLKKMKKLRLLSLQGISWINKLPNSIRTLSELRVLDLKS 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           C+NLE LP GIGSLK LTHLDV+GCYML+ MP SI KLT+LRVLKGFV G+S LN L+G 
Sbjct: 481 CFNLETLPQGIGSLKRLTHLDVSGCYMLDQMPTSISKLTELRVLKGFVAGRSSLNDLRGF 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETL----K 600
           KKLRKLSINT+ +DFP ETDL VLQDLGEHGKLR L I+W A+G+K        +     
Sbjct: 541 KKLRKLSINTNRRDFPDETDLRVLQDLGEHGKLRTLGIVWVAEGLKQPSSGERNMIRQVT 600

Query: 601 GKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIR 660
            KI R L+ + +K     N+   +LP +LEKLE+ECL KE+LP WLNP NL SLK+LYIR
Sbjct: 601 RKIGRQLTMQFSKQRDQSNDVILELPKELEKLEMECLPKEKLPLWLNPSNLTSLKRLYIR 660

Query: 661 GGSLKTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANG 718
           GG L  LG + W+ AEVVRLK+M +LKI+W +LQ++FP LSYLQKV CP+VTFCPCDANG
Sbjct: 661 GGKLAELGSEPWK-AEVVRLKHMTELKIEWRELQKSFPNLSYLQKVRCPRVTFCPCDANG 703

BLAST of Lsi09G004550 vs. NCBI nr
Match: XP_038907077.1 (probable disease resistance protein RF45 [Benincasa hispida])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 563/721 (78.09%), Postives = 630/721 (87.38%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPSPILD QTST IPTIPQMIE LS  H++LSTAL+K  A      TPTQAAG++IVP
Sbjct: 1   MASPSPILDPQTSTPIPTIPQMIETLSDLHKELSTALEKRSA------TPTQAAGDKIVP 60

Query: 61  A-VEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNR 120
           A VEPPAPT+ PEEQ G+KETEQ  E +G+ ETHKD KV  LL+NIQLL EAL+TMINNR
Sbjct: 61  AVVEPPAPTQTPEEQQGKKETEQVPEFNGSNETHKDPKVEILLNNIQLLMEALKTMINNR 120

Query: 121 DEKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKL 180
           D+KL+VPIQ IK +LD VIK V+EAPAGSPLTQ+IG+EYLDA++KD+RTLKF+IPSYR+L
Sbjct: 121 DKKLNVPIQSIKANLDTVIKMVKEAPAGSPLTQKIGEEYLDAINKDMRTLKFQIPSYRRL 180

Query: 181 SLAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENLKKCFRY 240
           SLAKTTAHFGDRGS+TRK +EFFRLPNLYG  VFD+S AF EIQT Y+  ++ LKKCF Y
Sbjct: 181 SLAKTTAHFGDRGSNTRKPDEFFRLPNLYGVDVFDESPAFKEIQTFYNGFDDLLKKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKKRFLT+WWIGE LLD  GTGDS+PE+ A  ILQ F +KGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKRFLTYWWIGEDLLDASGTGDSSPENKAGMILQKFADKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLA-KEKFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVRSAAI LA  E+FL YD GDNP     SCDRIFLVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRSAAIKLATAERFLKYDSGDNPTCESSSCDRIFLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K Q LEEK+GTIFNVSQPFPD ALEWLAKQG+ID+RTTKVVEWLLKL+NLKVLYLGR
Sbjct: 361 ---KNQKLEEKMGTIFNVSQPFPDSALEWLAKQGVIDIRTTKVVEWLLKLRNLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHV+EVESLEFLEGLKKMKKLRLLSLQGIFWIN+LPNSIN+L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVVEVESLEFLEGLKKMKKLRLLSLQGIFWINELPNSINRLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGF+TGKS LNYLKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFITGKSSLNYLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINTSS DFP + DL VL+DLGEHG+L+NL+I+W AQ VKS++  PET +G  V
Sbjct: 541 KKLRKLSINTSSHDFPNDNDLRVLRDLGEHGELQNLAIVWVAQEVKSNQQPPETARGMFV 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           +N SK+ +K TVPPN++T +LP KLEKLELECL  EELPNWLNP NLK+LKKLYIRGGSL
Sbjct: 601 QNFSKQFSKVTVPPNDETLELPKKLEKLELECLRVEELPNWLNPDNLKTLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 718
           K LGDKKWEAAEVVRLKYM +L+I W +LQ +FPKLSYLQKV+CP+VTFCPCDANGVWIK
Sbjct: 661 KKLGDKKWEAAEVVRLKYMTELQIDWRELQNSFPKLSYLQKVKCPRVTFCPCDANGVWIK 711

BLAST of Lsi09G004550 vs. NCBI nr
Match: KAA0043494.1 (putative disease resistance protein [Cucumis melo var. makuwa])

HSP 1 Score: 967.6 bits (2500), Expect = 5.9e-278
Identity = 513/721 (71.15%), Postives = 581/721 (80.58%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPS IL  Q ST IPTIPQMIE LS  +++LSTALQKH  QA                
Sbjct: 1   MASPSLILHPQNSTPIPTIPQMIETLSDLYKKLSTALQKHSTQA---------------- 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
             EP             K+T+Q  EISG+I+ +KD KV KL SNI LL EALE MINNRD
Sbjct: 61  --EP------------NKQTQQIPEISGSIDANKDPKVVKLQSNIMLLMEALEMMINNRD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           +KL+VPI+ I+ +LD+VIK+V +AP GSPLTQ+IG++YLDAV KDIR LKFRIPSYRKLS
Sbjct: 121 KKLNVPIRSIETNLDIVIKRVNDAPPGSPLTQKIGEDYLDAVIKDIRMLKFRIPSYRKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LAKT AH G RGS      EF  LPNL+GDKVFD+SSAF E+Q +Y   ++++ +KCF Y
Sbjct: 181 LAKTVAHSGGRGSHALTPIEFV-LPNLHGDKVFDESSAFKEVQKIYYEFSDDIFRKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKKR LT+WWIGEG+LD  GTGDSNPED A +IL  FVEKGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKRSLTYWWIGEGILDVSGTGDSNPEDKAGEILLKFVEKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLAKE-KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVRSA+I LA E KFLN+D+  NP      CD+I LVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRSASIKLAIENKFLNFDDWGNPTYRSFGCDKILLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K QNLE+ + TIFNVSQPFPD ALEWLAK+G +DMRTTKVVEWLLKL +LKVLYLGR
Sbjct: 361 ---KYQNLEKDLVTIFNVSQPFPDSALEWLAKKGDVDMRTTKVVEWLLKLDHLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHVIEV SLEFLEGLKKMK LRLLSLQGIFWIN+LPNSIN L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVIEVLSLEFLEGLKKMKTLRLLSLQGIFWINELPNSINMLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGFVTGKS LN LKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFVTGKSSLNDLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINT+S  FP   DL VL++LGEHG+LRNLSIMWAA+G+K D+P  +T KG  +
Sbjct: 541 KKLRKLSINTNSPGFPDAKDLRVLRELGEHGELRNLSIMWAAEGLKFDQPPSKTEKGTFI 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           R L+K+++K T  PN++T +LP  LEKLELECL ++ LPNWLNP NL SLKKLYIRGGSL
Sbjct: 601 RELAKQISKLTAQPNDETSELPKNLEKLELECLREKNLPNWLNPDNLTSLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 718
           +TLG+KKWEAAEVVRLKY+ KLKIKW +LQ NFPKLSYLQKV+CP VT  PCDANGVWIK
Sbjct: 661 ETLGNKKWEAAEVVRLKYITKLKIKWRELQNNFPKLSYLQKVKCPSVTLFPCDANGVWIK 686

BLAST of Lsi09G004550 vs. NCBI nr
Match: XP_016903724.1 (PREDICTED: uncharacterized protein LOC103482581 [Cucumis melo])

HSP 1 Score: 964.9 bits (2493), Expect = 3.8e-277
Identity = 512/720 (71.11%), Postives = 580/720 (80.56%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPS IL  Q ST IPTIPQMIE LS  +++LSTALQKH  QA                
Sbjct: 1   MASPSLILHPQNSTPIPTIPQMIETLSDLYKKLSTALQKHSTQA---------------- 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
             EP             K+T+Q  EISG+I+ +KD KV KL SNI LL EALE MINNRD
Sbjct: 61  --EP------------NKQTQQIPEISGSIDANKDPKVVKLQSNIMLLMEALEMMINNRD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           +KL+VPI+ I+ +LD+VIK+V +AP GSPLTQ+IG++YLDAV KDIR LKFRIPSYRKLS
Sbjct: 121 KKLNVPIRSIETNLDIVIKRVNDAPPGSPLTQKIGEDYLDAVIKDIRMLKFRIPSYRKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LAKT AH G RGS      EF  LPNL+GDKVFD+SSAF E+Q +Y   ++++ +KCF Y
Sbjct: 181 LAKTVAHSGGRGSHALTPIEFV-LPNLHGDKVFDESSAFKEVQKIYYEFSDDIFRKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKKR LT+WWIGEG+LD  GTGDSNPED A +IL  FVEKGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKRSLTYWWIGEGILDVSGTGDSNPEDKAGEILLKFVEKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLAKE-KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVRSA+I LA E KFLN+D+  NP      CD+I LVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRSASIKLAIENKFLNFDDWGNPTYRSFGCDKILLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K QNLE+ + TIFNVSQPFPD ALEWLAK+G +DMRTTKVVEWLLKL +LKVLYLGR
Sbjct: 361 ---KYQNLEKDLVTIFNVSQPFPDSALEWLAKKGDVDMRTTKVVEWLLKLDHLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHVIEV SLEFLEGLKKMK LRLLSLQGIFWIN+LPNSIN L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVIEVLSLEFLEGLKKMKTLRLLSLQGIFWINELPNSINMLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGFVTGKS LN LKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFVTGKSSLNDLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINT+S  FP   DL VL++LGEHG+LRNLSIMWAA+G+K D+P  +T KG  +
Sbjct: 541 KKLRKLSINTNSPGFPDAKDLRVLRELGEHGELRNLSIMWAAEGLKFDQPPSKTEKGTFI 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           R L+K+++K T  PN++T +LP  LEKLELECL ++ LPNWLNP NL SLKKLYIRGGSL
Sbjct: 601 RELAKQISKLTAQPNDETSELPKNLEKLELECLREKNLPNWLNPDNLTSLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 717
           +TLG+KKWEAAEVVRLKY+ KLKIKW +LQ NFPKLSYLQKV+CP VT  PCDANGVWIK
Sbjct: 661 ETLGNKKWEAAEVVRLKYITKLKIKWRELQNNFPKLSYLQKVKCPSVTLFPCDANGVWIK 685

BLAST of Lsi09G004550 vs. NCBI nr
Match: XP_004152418.1 (putative disease resistance protein At1g58400 [Cucumis sativus] >KGN50286.1 hypothetical protein Csa_000644 [Cucumis sativus])

HSP 1 Score: 963.0 bits (2488), Expect = 1.4e-276
Identity = 511/721 (70.87%), Postives = 579/721 (80.31%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MASPS IL  Q ST IPTIPQMIE LS  +++LSTALQKH  QA                
Sbjct: 1   MASPSLILHPQNSTPIPTIPQMIETLSDLYKKLSTALQKHTTQA---------------- 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
             EP             K T+Q   ISG I+  KD KV KL SNI LL EALETMINNRD
Sbjct: 61  --EP------------NKPTQQIPGISGTIDNDKDPKVLKLQSNIMLLMEALETMINNRD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           +KL+VPIQ I+ +LD+VIK+V +AP GSPLTQ+IG++YLDA+ +D+RTLKFRIPSYRKLS
Sbjct: 121 KKLNVPIQSIETNLDIVIKRVNDAPPGSPLTQKIGEDYLDAIIQDVRTLKFRIPSYRKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LAKT AH G RGS      EF  LPNL GD+VFD+S AF E+Q +Y   N+++ +KCF Y
Sbjct: 181 LAKTVAHSGGRGSHALTPIEFV-LPNLQGDEVFDESPAFKEVQKIYYEFNDDIFRKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FAVFPENVVLKK+FLT+WWIGEG+LD  GTGDSN ED A  IL  FVEKGLIVPVKEEQK
Sbjct: 241 FAVFPENVVLKKQFLTYWWIGEGILDVKGTGDSNLEDEAGGILLKFVEKGLIVPVKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLAKE-KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTE 360
           KVKKKFRMPPLVR A+I LA E KFLN+D+  NP      CDRI LVKGGGF PP+APT 
Sbjct: 301 KVKKKFRMPPLVRFASIKLAIENKFLNFDDWGNPTYRSFGCDRILLVKGGGFHPPEAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
              K QNLEEK+ TIFNVSQPFPD ALEWLAK+G +DMRTTKVVEWLLKL++LKVLYLGR
Sbjct: 361 ---KYQNLEEKMVTIFNVSQPFPDSALEWLAKKGDVDMRTTKVVEWLLKLEHLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQSEVD+EEHVIEV SLEFLEGL+KMKKLRLLSLQGIFWIN+LPNSIN L +LRVLDLKC
Sbjct: 421 WQSEVDDEEHVIEVLSLEFLEGLRKMKKLRLLSLQGIFWINELPNSINMLCDLRVLDLKC 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSI +LTQLRVLKGFVTGKS LN LKGL
Sbjct: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSISRLTQLRVLKGFVTGKSSLNDLKGL 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIV 600
           KKLRKLSINT+S  FP   DL VL++LGEHG+LRNLSIMWAA+G+K D+P  +T KG  +
Sbjct: 541 KKLRKLSINTNSPGFPDAKDLRVLRELGEHGELRNLSIMWAAEGLKFDQPPSKTEKGTFI 600

Query: 601 RNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSL 660
           R L+K+++K T  PN++T +LP  LEKLELECL ++ LPNWLNP NL SLKKLYIRGGSL
Sbjct: 601 RELTKQISKLTAQPNDETSELPKNLEKLELECLREKNLPNWLNPDNLTSLKKLYIRGGSL 660

Query: 661 KTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVWIK 718
           +TLG+KKWEAAEVVRLKYM +LKIKW +LQ NFPKLSYLQKV+CP+VT  PCDA+GVWIK
Sbjct: 661 ETLGNKKWEAAEVVRLKYMTELKIKWRELQNNFPKLSYLQKVKCPRVTLFPCDASGVWIK 686

BLAST of Lsi09G004550 vs. NCBI nr
Match: XP_023533578.1 (disease resistance RPP13-like protein 4 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.4 bits (2149), Expect = 2.9e-237
Identity = 463/725 (63.86%), Postives = 538/725 (74.21%), Query Frame = 0

Query: 1   MASPSPILDSQTSTSIPTIPQMIEILSKSHEQLSTALQKHFAQAKSTTTPTQAAGNEIVP 60
           MAS SP L  Q S  IPTIPQMIE LSK H +LSTAL       K +TT  QAA N   P
Sbjct: 1   MASRSPPLHPQASDLIPTIPQMIESLSKLHGELSTAL-------KESTTRAQAAANRRAP 60

Query: 61  AVEPPAPTEAPEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKEALETMINNRD 120
                    A  +   E+      E + +I  +++ +  KLL+NI+ LK ALE   N  D
Sbjct: 61  ---------ASADDQIEEAARPHTETNASIRANQNPQFEKLLTNIEHLKGALEA-TNELD 120

Query: 121 EKLSVPIQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLS 180
           ++L  PIQ IK SL+ VIK VE       L Q+I + +LDAVSKD++TLKF+IPSY+KLS
Sbjct: 121 KQLKEPIQSIKGSLEEVIKSVE-------LDQKIEENFLDAVSKDMKTLKFQIPSYQKLS 180

Query: 181 LAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTVYDALNENL-KKCFRY 240
           LA  TAH  D+G  T + +E F+LPNLY  KVFD+S AFIEIQ +Y+   +++ KKCF Y
Sbjct: 181 LAGNTAHLVDKGGGTARQDE-FKLPNLYAHKVFDESPAFIEIQDIYNGFGDDICKKCFLY 240

Query: 241 FAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQK 300
           FA FPENVVLKKR LTHWWIGEGLLD  G+GD  PE  A +IL+ F EKGLIVP+KEEQK
Sbjct: 241 FAAFPENVVLKKRLLTHWWIGEGLLDSSGSGDQMPEVRAGEILKKFAEKGLIVPLKEEQK 300

Query: 301 KVKKKFRMPPLVRSAAINLA-KEKFLNYDEGDNPIGIS--CDRIFLVKGGGFCPPKAPTE 360
           KVKKKF MPP+VRSAAI LA KE+FLNYD GDNPIG S  CDRIFLVKGGG   PKAPT 
Sbjct: 301 KVKKKFTMPPIVRSAAIILAKKEQFLNYDSGDNPIGKSSGCDRIFLVKGGGLRQPKAPT- 360

Query: 361 ERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGR 420
                QNLE  + +IFNVSQPFPD ALEWLAK+G +DMRT KVVEWLLKLKNLKVLYLGR
Sbjct: 361 -LTTDQNLEVTMESIFNVSQPFPDSALEWLAKKGEVDMRTDKVVEWLLKLKNLKVLYLGR 420

Query: 421 WQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKC 480
           WQS VDE++  IEVESLEFLEGLKKMKKLRLLSLQGI WINKLPNSI  L ELRVLDLK 
Sbjct: 421 WQSAVDEQQ--IEVESLEFLEGLKKMKKLRLLSLQGISWINKLPNSIRTLSELRVLDLKS 480

Query: 481 CYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLNYLKGL 540
           C+NLE LP GIGSLK LTHLDV+GCYML+ MP SI KLT+LRVLKGFVTG+S LN L+G 
Sbjct: 481 CFNLETLPQGIGSLKRLTHLDVSGCYMLDQMPTSISKLTELRVLKGFVTGRSSLNDLRGF 540

Query: 541 KKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETL----K 600
           KKLRKLSINT+ +DFP ETDL VLQDLGEHGKLR L I+W A+G+K        +     
Sbjct: 541 KKLRKLSINTNRRDFPDETDLRVLQDLGEHGKLRTLGIVWVAEGLKQPSSGERNMIRQVT 600

Query: 601 GKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIR 660
            KI R L+ +L+K     N+   +LP +LEKLE+ECL KE+LP WLNP NL SLK+LYIR
Sbjct: 601 RKIGRQLTMQLSKQRDQSNDVILELPKELEKLEMECLPKEKLPLWLNPSNLTSLKRLYIR 660

Query: 661 GGSLKTLGDKKWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANG 718
           GG L  LG++ W+ AEVVRLKYM +LKI+W +LQ++FP LSYLQKV CP+VTFCPCDANG
Sbjct: 661 GGKLAELGNEPWK-AEVVRLKYMTELKIEWRELQKSFPNLSYLQKVRCPRVTFCPCDANG 695

BLAST of Lsi09G004550 vs. TAIR 10
Match: AT5G45500.1 (RNI-like superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 4.1e-88
Identity = 226/688 (32.85%), Postives = 358/688 (52.03%), Query Frame = 0

Query: 70  APEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKE---ALETMINNRDEKLSVP 129
           A + Q      E A   +   E   D  + KL  N++LLKE    L+ + +   E++   
Sbjct: 97  ADQPQTNLGRAESAPIPNAKPEGPDDHHIPKLQRNLRLLKEDVAKLQYLRSEVAEEVWKH 156

Query: 130 IQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLSLAKTTA 189
           I+ ++  L+ V K+ ++ P   PLT  + K+ LD ++K I  L  ++P            
Sbjct: 157 IRPLELLLENVAKEQKKNPTKPPLTLGMKKD-LDDINKKIFNLMCQVPLLPNKRKKAKGL 216

Query: 190 HFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSA-FIEIQTVYDALNENLKKCFRYFAVFPE 249
              D   D         LP ++ +K   +S A F  +   ++ L++  K C   FAVFPE
Sbjct: 217 DDDDGDGDNNNGKGIVCLPGIHANKADLRSRAVFRYVLREFEELSDQRKICLLTFAVFPE 276

Query: 250 NVVLKKRFLTHWWIGEGLLD--DLGTGDS------NPEDGASKILQIFVEKGLIVPVKEE 309
           N  + +  L +WW+GEG+L   D+ + +        PED    IL+ F ++ LI PV+ +
Sbjct: 277 NQEVNRTMLMYWWMGEGILSTKDISSQEGTEEVILKPEDVVKVILKDFTDRNLIEPVEIK 336

Query: 310 QKKVKKKFRMPPLVRSAAINLAKE--KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKA 369
           +K     ++M P V ++ + ++KE   F  YD  D P+       ++ LV+G       +
Sbjct: 337 RKVEPSSYKMAPFVHASVVLISKEIGLFDMYDIKDKPVMKKSGMHKVCLVEG-------S 396

Query: 370 PTEERMKGQNLE--EKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKV 429
            +++  K + +E  + I T+FNVS+ FPD   +W ++         K+    +  + LKV
Sbjct: 397 SSQQEAKAKKMEDVDHIETVFNVSERFPDFTFKWFSED---KPTRNKLTLSKVTYQKLKV 456

Query: 430 LYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRV 489
            YLGRW+         IEVE+ E ++ LK+M KL+LLS QGI  I +L +++ KL +L +
Sbjct: 457 FYLGRWERTAKRH---IEVENPELMKNLKRMIKLKLLSFQGISRIERLDDAVCKLRDLII 516

Query: 490 LDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKS--- 549
           LDL+ CYNLEKLP  I SLK+L +LD+T CYM++ MPK +  L  L VLKGFV   +   
Sbjct: 517 LDLRACYNLEKLPDKIDSLKALIYLDITDCYMIDRMPKRLSWLDNLEVLKGFVVSDATDE 576

Query: 550 ----GLNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWA------- 609
                L  L  LKKLRKLSI+ + ++F  +   + ++      KL  L + W        
Sbjct: 577 ETVCTLAELVHLKKLRKLSISINKENFSIDDVFVAVKSF---KKLEKLKVAWGGINTHPQ 636

Query: 610 AQGVKSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNW 669
            +GV S++ D      + +  L +         +   P+LP  L+KL+L+C   E LP W
Sbjct: 637 GKGVDSEKGDENVKPKENIGILERAATMFRKEKDPTAPELPKTLKKLDLQCFPGEHLPEW 696

Query: 670 LNPLNLKSLKKLYIRGGSLKTLGDKKWEAAE-------VVRLKYMQKLKIKWGKLQEN-F 718
           L P NL +++KLYI+GG +K  G  K   ++       V+RLK++ KLK++W  L +  F
Sbjct: 697 LEPDNLLNVEKLYIKGG-IKLTGFGKSLPSQNSKCKVTVLRLKFLPKLKVEWRDLSKRYF 756

BLAST of Lsi09G004550 vs. TAIR 10
Match: AT5G45500.2 (RNI-like superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 4.1e-88
Identity = 226/688 (32.85%), Postives = 358/688 (52.03%), Query Frame = 0

Query: 70  APEEQHGEKETEQAQEISGNIETHKDLKVGKLLSNIQLLKE---ALETMINNRDEKLSVP 129
           A + Q      E A   +   E   D  + KL  N++LLKE    L+ + +   E++   
Sbjct: 97  ADQPQTNLGRAESAPIPNAKPEGPDDHHIPKLQRNLRLLKEDVAKLQYLRSEVAEEVWKH 156

Query: 130 IQIIKDSLDVVIKKVEEAPAGSPLTQQIGKEYLDAVSKDIRTLKFRIPSYRKLSLAKTTA 189
           I+ ++  L+ V K+ ++ P   PLT  + K+ LD ++K I  L  ++P            
Sbjct: 157 IRPLELLLENVAKEQKKNPTKPPLTLGMKKD-LDDINKKIFNLMCQVPLLPNKRKKAKGL 216

Query: 190 HFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSA-FIEIQTVYDALNENLKKCFRYFAVFPE 249
              D   D         LP ++ +K   +S A F  +   ++ L++  K C   FAVFPE
Sbjct: 217 DDDDGDGDNNNGKGIVCLPGIHANKADLRSRAVFRYVLREFEELSDQRKICLLTFAVFPE 276

Query: 250 NVVLKKRFLTHWWIGEGLLD--DLGTGDS------NPEDGASKILQIFVEKGLIVPVKEE 309
           N  + +  L +WW+GEG+L   D+ + +        PED    IL+ F ++ LI PV+ +
Sbjct: 277 NQEVNRTMLMYWWMGEGILSTKDISSQEGTEEVILKPEDVVKVILKDFTDRNLIEPVEIK 336

Query: 310 QKKVKKKFRMPPLVRSAAINLAKE--KFLNYDEGDNPI--GISCDRIFLVKGGGFCPPKA 369
           +K     ++M P V ++ + ++KE   F  YD  D P+       ++ LV+G       +
Sbjct: 337 RKVEPSSYKMAPFVHASVVLISKEIGLFDMYDIKDKPVMKKSGMHKVCLVEG-------S 396

Query: 370 PTEERMKGQNLE--EKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKV 429
            +++  K + +E  + I T+FNVS+ FPD   +W ++         K+    +  + LKV
Sbjct: 397 SSQQEAKAKKMEDVDHIETVFNVSERFPDFTFKWFSED---KPTRNKLTLSKVTYQKLKV 456

Query: 430 LYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINKLGELRV 489
            YLGRW+         IEVE+ E ++ LK+M KL+LLS QGI  I +L +++ KL +L +
Sbjct: 457 FYLGRWERTAKRH---IEVENPELMKNLKRMIKLKLLSFQGISRIERLDDAVCKLRDLII 516

Query: 490 LDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVTGKS--- 549
           LDL+ CYNLEKLP  I SLK+L +LD+T CYM++ MPK +  L  L VLKGFV   +   
Sbjct: 517 LDLRACYNLEKLPDKIDSLKALIYLDITDCYMIDRMPKRLSWLDNLEVLKGFVVSDATDE 576

Query: 550 ----GLNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWA------- 609
                L  L  LKKLRKLSI+ + ++F  +   + ++      KL  L + W        
Sbjct: 577 ETVCTLAELVHLKKLRKLSISINKENFSIDDVFVAVKSF---KKLEKLKVAWGGINTHPQ 636

Query: 610 AQGVKSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNW 669
            +GV S++ D      + +  L +         +   P+LP  L+KL+L+C   E LP W
Sbjct: 637 GKGVDSEKGDENVKPKENIGILERAATMFRKEKDPTAPELPKTLKKLDLQCFPGEHLPEW 696

Query: 670 LNPLNLKSLKKLYIRGGSLKTLGDKKWEAAE-------VVRLKYMQKLKIKWGKLQEN-F 718
           L P NL +++KLYI+GG +K  G  K   ++       V+RLK++ KLK++W  L +  F
Sbjct: 697 LEPDNLLNVEKLYIKGG-IKLTGFGKSLPSQNSKCKVTVLRLKFLPKLKVEWRDLSKRYF 756

BLAST of Lsi09G004550 vs. TAIR 10
Match: AT5G45520.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 231.1 bits (588), Expect = 2.8e-60
Identity = 179/543 (32.97%), Postives = 276/543 (50.83%), Query Frame = 0

Query: 91  ETHKDLKVGKLLSNIQLLKEALETMIN-NRDEKLSVPIQIIKDSLDVVIKKVEEAPAGSP 150
           + ++DL++ KL ++I+ +  A + +     D   ++   +    L  +++K     + S 
Sbjct: 91  QKNEDLEIRKLQNDIRQMIAAFKNLTQFQTDMSKNLERDLRSTKLIAILQKKNSFGSRSH 150

Query: 151 LTQQIGKEYLDAVSKDIRTLKFRIPSY------RKLSLAKTTAHFGDRGSDTRKSNEFFR 210
           + ++I ++        +  LK +IPS       RK+S+  T +   +   +  ++     
Sbjct: 151 VVKEIRRK--------VSALKCQIPSLLHKQSSRKISI--TDSQTAEENDEISETGIDIY 210

Query: 211 LPNLYGDKVFDKSSAFIEIQTVYDALNENLKK-CFRYFAVFPENVVLKKRFLTHWWIGEG 270
           LP L+  + F  SSAF E+   +  L++  +K C   FAVFPEN  +K+  L +WWIGEG
Sbjct: 211 LPGLHFSEKFKTSSAFEEVVEKFQGLDDFTQKLCLLSFAVFPENREVKRTMLMYWWIGEG 270

Query: 271 LLDDLGTGDSNPEDGASKILQIFVEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEK 330
            +        + E+  ++IL  F +K L+ PV++E+K +   ++M P V SA I LAKE 
Sbjct: 271 FI-----SCDDSENLVTRILDSFSDKKLLEPVEDERKLLPSSYKMEPHVHSAVIYLAKEM 330

Query: 331 --FLNYDEGDNPI--GISCDRIFLVKGGGFCPPKAPTEERMKGQNLEEK-IGTIFNVSQP 390
             F  Y++    I    S  ++ LVKG              K   +E K + T+FN S+ 
Sbjct: 331 DLFELYNKNGKLIMKKSSKKKVCLVKGSSLL-------RDAKTSVMEPKTLQTVFNSSER 390

Query: 391 FPDLALEWLAKQGIIDMRTTKVVEWLLKLKNLKVLYLGRWQSEVDEEEHVIEVESLEFLE 450
           +PD   +W                    + +L+VLYLGRW+         IEVES EFL+
Sbjct: 391 YPDFTFKWFP-----------------LMDSLRVLYLGRWEQTAKRH---IEVESTEFLK 450

Query: 451 GLKKMKKLRLLSLQGIFWINKLPNSINKLGELRVLDLKCCYNLEKLPGGIGSLKSLTHLD 510
            +K +K LRL S QGI  I +L NSI  L EL +LDLK CYNLE LP  IG  + L +LD
Sbjct: 451 NMKSLKNLRLASFQGISRIERLENSICALPELVILDLKACYNLEVLPSDIGLFEKLIYLD 510

Query: 511 VTGCYMLNGMPKSIYKLTQLRVLKGFVTGKSGLN---YLKGLKKLRKLSINTSSQDFPKE 570
           V+ CYML+ MPK I KL++L+VLKGFV  +S       +K L  LRKLSI  +   F  E
Sbjct: 511 VSECYMLDRMPKGIAKLSRLQVLKGFVISESDHENNCAVKHLVNLRKLSITVNKYSFKVE 570

Query: 571 TDLLVLQDLGEHGKLRNLSIMWAAQGVKSDEPDPETLKGKIVRNLSKKLNKATVPPNNKT 618
           +   +++ L     L +L I W A     +EP+ E ++ +   N  KK N+  +  + K 
Sbjct: 571 S---LMESLTGLQGLESLKIAWGA----CEEPN-EAVESRETTN-EKKENQGGLATDKKL 582


HSP 2 Score: 117.1 bits (292), Expect = 5.9e-26
Identity = 55/112 (49.11%), Postives = 78/112 (69.64%), Query Frame = 0

Query: 609  PPNNKTPDLPTKLEKLELECLWKEELPNWLNPLNLKSLKKLYIRGGSLKTLGDK------ 668
            P  + T  LP+ L+KLELEC  + + P+WLNP +LK+LKKL I+GG L  +GD+      
Sbjct: 1051 PVVDDTYTLPSNLKKLELECFPETKPPSWLNPKDLKNLKKLSIKGGKLSRIGDESRITED 1110

Query: 669  KWEAAEVVRLKYMQKLKIKWGKLQENFPKLSYLQKVECPKVTFCPCDANGVW 715
            KW+  E++RLKY+ + K++W  LQ  FPK++ L+K +CPK+ FCP D NGVW
Sbjct: 1111 KWD-VEILRLKYLHEFKVEWRDLQTLFPKMTLLEKYKCPKIAFCPTDGNGVW 1161

BLAST of Lsi09G004550 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 124.4 bits (311), Expect = 3.7e-28
Identity = 150/625 (24.00%), Postives = 256/625 (40.96%), Query Frame = 0

Query: 110 EALETMINNRDEKLSVPIQIIKDSLDVVIKKV----EEAPAGSPLTQQIGKEYLDAVSKD 169
           E++   +  RD+K   P  +  D+  ++   V     +     P  + +GKE    +   
Sbjct: 299 ESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKE----IVTK 358

Query: 170 IRTLKFRIPSYRKLSLAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTV 229
            + L   I +   L L K   +      + R+  E F+   L G+   +  +    +Q  
Sbjct: 359 CKGLPLTIKAVGGLLLCKDHVY-----HEWRRIAEHFQ-DELRGN-TSETDNVMSSLQLS 418

Query: 230 YDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIF 289
           YD L  +LK C    +++PE+ V+ K+ L H WIGEG +  +     +  +         
Sbjct: 419 YDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFV--MWRNGRSATESGEDCFSGL 478

Query: 290 VEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEKFLNYDEGDNPIGISCDRIFLVKG 349
             + LI  V +         ++  +VR   I++AK+     D   NP G++C  + +   
Sbjct: 479 TNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKK-----DSFSNPEGLNCRHLGI--S 538

Query: 350 GGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLK 409
           G F   +     +++G     K G +  ++    DLA ++                    
Sbjct: 539 GNFDEKQIKVNHKLRGVVSTTKTGEVNKLNS---DLAKKF------------------TD 598

Query: 410 LKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINK 469
            K L+VL         D  + + +    E L+ +  ++ L  LSL     + + P S+  
Sbjct: 599 CKYLRVL---------DISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMED 658

Query: 470 LGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVT 529
           L  L++LD   C NL++L   I   K L  LD+T C  L   PK I  L +L VL GF  
Sbjct: 659 LHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKP 718

Query: 530 GKSG----LNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGV 589
            +S     L+ +K L  LRKL ++ +  D  +E +L  L +L    KL ++SI       
Sbjct: 719 ARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINL---SKLMSISI------- 778

Query: 590 KSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPL 649
              +   + L  KI          A  PP+        +L +L L+    +  P+WL+P 
Sbjct: 779 NCYDSYGDDLITKI---------DALTPPH--------QLHELSLQFYPGKSSPSWLSPH 838

Query: 650 NLKSLKKLYIRGGSLKTLGDKKWE------AAEVVRLKYMQKLKIKWGKLQENFPKLSYL 709
            L  L+ + I  G+L  + +  W         E + L  +  L + W  LQ++ P L  +
Sbjct: 839 KLPMLRYMSICSGNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDWEVLQQSMPYLRTV 846

Query: 710 QKVECPKV-TFCPCDA---NGVWIK 717
               CP++ +F   D     GVW+K
Sbjct: 899 TANWCPELESFAIEDVGFRGGVWMK 846

BLAST of Lsi09G004550 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 124.4 bits (311), Expect = 3.7e-28
Identity = 150/625 (24.00%), Postives = 256/625 (40.96%), Query Frame = 0

Query: 110 EALETMINNRDEKLSVPIQIIKDSLDVVIKKV----EEAPAGSPLTQQIGKEYLDAVSKD 169
           E++   +  RD+K   P  +  D+  ++   V     +     P  + +GKE    +   
Sbjct: 299 ESVAKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKE----IVTK 358

Query: 170 IRTLKFRIPSYRKLSLAKTTAHFGDRGSDTRKSNEFFRLPNLYGDKVFDKSSAFIEIQTV 229
            + L   I +   L L K   +      + R+  E F+   L G+   +  +    +Q  
Sbjct: 359 CKGLPLTIKAVGGLLLCKDHVY-----HEWRRIAEHFQ-DELRGN-TSETDNVMSSLQLS 418

Query: 230 YDALNENLKKCFRYFAVFPENVVLKKRFLTHWWIGEGLLDDLGTGDSNPEDGASKILQIF 289
           YD L  +LK C    +++PE+ V+ K+ L H WIGEG +  +     +  +         
Sbjct: 419 YDELPSHLKSCILTLSLYPEDCVIPKQQLVHGWIGEGFV--MWRNGRSATESGEDCFSGL 478

Query: 290 VEKGLIVPVKEEQKKVKKKFRMPPLVRSAAINLAKEKFLNYDEGDNPIGISCDRIFLVKG 349
             + LI  V +         ++  +VR   I++AK+     D   NP G++C  + +   
Sbjct: 479 TNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIAKK-----DSFSNPEGLNCRHLGI--S 538

Query: 350 GGFCPPKAPTEERMKGQNLEEKIGTIFNVSQPFPDLALEWLAKQGIIDMRTTKVVEWLLK 409
           G F   +     +++G     K G +  ++    DLA ++                    
Sbjct: 539 GNFDEKQIKVNHKLRGVVSTTKTGEVNKLNS---DLAKKF------------------TD 598

Query: 410 LKNLKVLYLGRWQSEVDEEEHVIEVESLEFLEGLKKMKKLRLLSLQGIFWINKLPNSINK 469
            K L+VL         D  + + +    E L+ +  ++ L  LSL     + + P S+  
Sbjct: 599 CKYLRVL---------DISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLIQFPRSMED 658

Query: 470 LGELRVLDLKCCYNLEKLPGGIGSLKSLTHLDVTGCYMLNGMPKSIYKLTQLRVLKGFVT 529
           L  L++LD   C NL++L   I   K L  LD+T C  L   PK I  L +L VL GF  
Sbjct: 659 LHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKP 718

Query: 530 GKSG----LNYLKGLKKLRKLSINTSSQDFPKETDLLVLQDLGEHGKLRNLSIMWAAQGV 589
            +S     L+ +K L  LRKL ++ +  D  +E +L  L +L    KL ++SI       
Sbjct: 719 ARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINL---SKLMSISI------- 778

Query: 590 KSDEPDPETLKGKIVRNLSKKLNKATVPPNNKTPDLPTKLEKLELECLWKEELPNWLNPL 649
              +   + L  KI          A  PP+        +L +L L+    +  P+WL+P 
Sbjct: 779 NCYDSYGDDLITKI---------DALTPPH--------QLHELSLQFYPGKSSPSWLSPH 838

Query: 650 NLKSLKKLYIRGGSLKTLGDKKWE------AAEVVRLKYMQKLKIKWGKLQENFPKLSYL 709
            L  L+ + I  G+L  + +  W         E + L  +  L + W  LQ++ P L  +
Sbjct: 839 KLPMLRYMSICSGNLVKMQEPFWGNENTHWRIEGLMLSSLSDLDMDWEVLQQSMPYLRTV 846

Query: 710 QKVECPKV-TFCPCDA---NGVWIK 717
               CP++ +F   D     GVW+K
Sbjct: 899 TANWCPELESFAIEDVGFRGGVWMK 846

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388345.2e-2724.00Disease resistance RPP13-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RPP13... [more]
Q7XA424.6e-1525.51Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q9LVT45.2e-1123.30Probable disease resistance protein At5g47250 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q7XA402.0e-1022.72Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R... [more]
Q8W3K34.4e-1022.43Putative disease resistance protein At1g58400 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7TJK32.8e-27871.15Putative disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S4E6781.8e-27771.11uncharacterized protein LOC103482581 OS=Cucumis melo OX=3656 GN=LOC103482581 PE=... [more]
A0A0A0KRD77.0e-27770.87Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G165320 PE=4 SV=1[more]
A0A6J1K7N65.1e-23563.45disease resistance RPP13-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111491... [more]
A0A6J1H2K46.6e-23563.45probable disease resistance protein At1g12280 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038907077.10.0e+0078.09probable disease resistance protein RF45 [Benincasa hispida][more]
KAA0043494.15.9e-27871.15putative disease resistance protein [Cucumis melo var. makuwa][more]
XP_016903724.13.8e-27771.11PREDICTED: uncharacterized protein LOC103482581 [Cucumis melo][more]
XP_004152418.11.4e-27670.87putative disease resistance protein At1g58400 [Cucumis sativus] >KGN50286.1 hypo... [more]
XP_023533578.12.9e-23763.86disease resistance RPP13-like protein 4 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G45500.14.1e-8832.85RNI-like superfamily protein [more]
AT5G45500.24.1e-8832.85RNI-like superfamily protein [more]
AT5G45520.12.8e-6032.97Leucine-rich repeat (LRR) family protein [more]
AT3G50950.23.7e-2824.00HOPZ-ACTIVATED RESISTANCE 1 [more]
AT3G50950.13.7e-2824.00HOPZ-ACTIVATED RESISTANCE 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 221..317
e-value: 1.8E-10
score: 42.8
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 386..709
e-value: 7.6E-27
score: 95.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..85
NoneNo IPR availablePANTHERPTHR23155:SF1076LEUCINE-RICH REPEAT (LRR) FAMILY PROTEIN-RELATEDcoord: 131..697
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 397..706
IPR044974Disease resistance protein, plantsPANTHERPTHR23155DISEASE RESISTANCE PROTEIN RPcoord: 131..697

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G004550.1Lsi09G004550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response