Lsi09G004380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G004380
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDNA mismatch repair protein MSH4
Locationchr09: 4645691 .. 4648681 (+)
RNA-Seq ExpressionLsi09G004380
SyntenyLsi09G004380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACCAGCAGCTCCTACCAGAATACAAAAACTTTGCTGCATTTCTATGATCCAATGGTTATACTTGTTCCTCCAAACAAGCTTGCACCTGATGGCATGGTTGGAGTTTCAGTTTTGGCCGATAGATTTTTTCCTACAGTGAAGAAGGTTAACTTAAATTCCTTGTCTGTGGAAATTGCTTACCTTTGTGGAGAAACTTAAATTGAATTTAAAATTCTGTAGGTTGTAATGGCGCGTGTTTGCTTTGATGACACAAAGGTTTTTTTTCCTGTCTTTTTTCTTTTAAAGTTTTATTTGATGGTTACTGTATTGGATGCTGTTCTTTTATCAGTCTGCTTAGAACATATCTTACTTGAACTTTCCAGGGTGCTGTTCTGATTAAGAATCTTTCAGCCAAGGAACCTTCTGCTCTTGGTTTAGAAACTTATTACAAACAGTACTATCTCTGTCTGGCTGCTGCTGCTGCCAGCATTAAATGGTATTATCCGAACTCGAGTCTTCTACTACTTTGGTATGAGAAGTGTTGGTTAGGTTAGTTTGTTAACAAATAAGAAGAGAGGAAAAGTGTCAGTCTTATGATGCTAATATTTTCTTGAGAGTATTCTTGTTGGCTTGAATGCTCAGCTTTCACTAATGAACCAGCTTCTTGATGCTCAGGATTGAAGCAGAGAAAGGGGTAATCGTGACCAATCACTCTTTATCGGTAAATTTGCCTCTCTGAGATGGTATTAGAAATGAATAGAATTCAGTGGTTTAACCTTTCTCCCGATGCTTGAATGAGATTCACTGTATGAAAGATAATCTCACAATAACCAATATTACTGAATTAATTAGTCAAGTTAAGTTTTTGTAGTTTTGTAAGACGGTTACTCAACTTCTCTACCTCATCTTGGTATGTGTATATGATGTATTCTGTATTTTTATTTTAGTTGGTTGTTGAATCTTTAAGTTTAGTTACTTCTTTAGTTAGTTTTTTAATTACTTTCTTGTATTTCTTTAATCCAATGGTCGGCGCCTAAAAAATTCTTCTTCTTCCGATGAAAATTCAGAATCTTGATCCTCCAAGATTGGTTCTTGAAATCTTGGAAGTCGAAAAAGTAGTGGATTGCCGTTTGGGGAATTGATATTGGTTTGAATACCTCTTCTTGGCAATCTTTCCTCTGTTTCTTGGAGAGGTCTCCTCAATCTCTCCTCTCCTTTGTTTTCTTCCGGTTGTCTTGGAATGGTTGTCAAAACTTGTTCCATTTTCTTGATATTCGGTTTATTGCTAAGATCAATTCAGCAATATTGGTGTTTATTTCTCCAATGGATTCTTCTACAGCCAACAATCGGGCAGTAGCTGTCTTGGGAGAAAAAAACTCGTCCTGTTCGGGATGGTTTTCTGCCGCCTTCCCTTGCCGGTGTCCGCTGCGATTTTTCCAGCCACGAAGTCCCAAATTCGAAGGGCTCTGATACCAAACTTGATGTGTCAAGTTGAGAGAGAAGACTAAATTCTGAAAATGCTTCAAAATTGTATTCAAAATGATTGAGAACAGTAACAATTTAAAAGAATTAGTTACCAACAGTAACTAAAGAATTTAAAGAAATACAAGAAAGTAATTAAAGAACTAACTAAAGAAGTAACTAAACTTAAAGATTCAGCAACCAACAAAAATAAAAATACAGAATACATCAGTATAAGATTGCTGACCTAAGATAGGGAAATTAATTATTTAATTTTATTGTCCTTTCATCTTGGTTGAGTAAATAAAAAAAGACACACCTTATTTCTTACGTTATTCTCTTCTTTACAGAGGTTAATCAGTCAATTTATTGTATTATTCGAAATATGGTTGCTCCAGTAGGAGCGTACTGAGCCTCTCTTCTATGACATTCTTTGCTTTTCATTTTCTACATTACTTCGCAAAGAAATTTCTTTTCCCTATAGCATCTTTGATTGGTGAAGCTCTGTTGCTCCACACCTGTAGGATTACTATATGCGCAAAGATTGTTTATATCATCTGTAATTATAACCACTAGACAAGGATTTTCCTACATATTGGTTGAGTCACGAATTTTAAACATATCAAACTGAATTTAAGGTTCTGACTCCCTGTTAATACAGTTTTTTTTTTTCAAATGATAATAATAATTCTGTTACTTCTATTTTTACTACAGCTCATTCCTTTTCACTTTTTGACTTCTGTTCTTCTATTTTCTGGACTTGTGTTTTGAAAGATAAAGTACTATGCACAGAAGATCCTATCATCTGGAAATGTAATTAACTTTAGGGACTAGGCCTAAAATATTTGTTCTCTTTTTGACTTCAAATATCAGTTTTTATTTGTCTTCTGAATGAATTCATAAACCTTACATTTGGTTCTGGAGTTCTTACTCCATCCTGATGGTTCAGTTGTAAATTTTAGGTCACCTTCAATGGCTCATCTGATCACGTGAGCATTGATGCAACGAGGTTCCCCTCATTTCTTTTTTCTTTACAACTATGTGTAGCTAATCAAGATGACTGCGGATTTCTGATTAAATGCTGAATTGTTTGGTGGCAGCGTTCAGAATTTAGAAATTATTGAGCCACTTCACTCCAACCTTTGGGGAACAAGCAACAAGAAGAGAAGTCTGTACAACATGCTCAAGACAACAAAAACTATAGGAGGGTACGGGAATACACGTTGGTTGATTTTTTATTTTCCACCTGTTGCTGCAAAATTGATCGTCTTACCTCATCATGTTTTTATCTAAACTAGTATTTGATGACATGCAATTGAATATCACGTAGATGAGTTGTTAATACCAAAGAGAATTATTTTGACATACAGTTATTGCCAAGTACTCGTGTAAATACGTATGTATTTAGTCCCTTTCGCATGTTTATTTTAAAATTTGTGCTTTACTATCACTGCAACGGTCAAGTTTTTGTTACAGGTCTAGACTTCTTCGTGCCAATCTTTTGCAGCCACTAAAAGATATTGAAACCATTAATGCCCGTCTGGATTGC

mRNA sequence

AGACCAGCAGCTCCTACCAGAATACAAAAACTTTGCTGCATTTCTATGATCCAATGGTTATACTTGTTCCTCCAAACAAGCTTGCACCTGATGGCATGGTTGGAGTTTCAGTTTTGGCCGATAGATTTTTTCCTACAGTGAAGAAGGTTGTAATGGCGCGTGTTTGCTTTGATGACACAAAGGGTGCTGTTCTGATTAAGAATCTTTCAGCCAAGGAACCTTCTGCTCTTGGTTTAGAAACTTATTACAAACAGTACTATCTCTGTCTGGCTGCTGCTGCTGCCAGCATTAAATGGTATTATCCGAACTCGAGTCTTCTACTACTTTGGTATGAGAACTTCTTGATGCTCAGGATTGAAGCAGAGAAAGGGGTAATCGTGACCAATCACTCTTTATCGATAAAGTACTATGCACAGAAGATCCTATCATCTGGAAATGTCACCTTCAATGGCTCATCTGATCACGTGAGCATTGATGCAACGAGCGTTCAGAATTTAGAAATTATTGAGCCACTTCACTCCAACCTTTGGGGAACAAGCAACAAGAAGAGAAGTCTGTACAACATGCTCAAGACAACAAAAACTATAGGAGGGTCTAGACTTCTTCGTGCCAATCTTTTGCAGCCACTAAAAGATATTGAAACCATTAATGCCCGTCTGGATTGC

Coding sequence (CDS)

ATGGTTATACTTGTTCCTCCAAACAAGCTTGCACCTGATGGCATGGTTGGAGTTTCAGTTTTGGCCGATAGATTTTTTCCTACAGTGAAGAAGGTTGTAATGGCGCGTGTTTGCTTTGATGACACAAAGGGTGCTGTTCTGATTAAGAATCTTTCAGCCAAGGAACCTTCTGCTCTTGGTTTAGAAACTTATTACAAACAGTACTATCTCTGTCTGGCTGCTGCTGCTGCCAGCATTAAATGGTATTATCCGAACTCGAGTCTTCTACTACTTTGGTATGAGAACTTCTTGATGCTCAGGATTGAAGCAGAGAAAGGGGTAATCGTGACCAATCACTCTTTATCGATAAAGTACTATGCACAGAAGATCCTATCATCTGGAAATGTCACCTTCAATGGCTCATCTGATCACGTGAGCATTGATGCAACGAGCGTTCAGAATTTAGAAATTATTGAGCCACTTCACTCCAACCTTTGGGGAACAAGCAACAAGAAGAGAAGTCTGTACAACATGCTCAAGACAACAAAAACTATAGGAGGGTCTAGACTTCTTCGTGCCAATCTTTTGCAGCCACTAAAAGATATTGAAACCATTAATGCCCGTCTGGATTGC

Protein sequence

MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALGLETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYAQKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGSRLLRANLLQPLKDIETINARLDC
Homology
BLAST of Lsi09G004380 vs. ExPASy Swiss-Prot
Match: F4JP48 (DNA mismatch repair protein MSH4 OS=Arabidopsis thaliana OX=3702 GN=MSH4 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.0e-65
Identity = 136/203 (67.00%), Postives = 154/203 (75.86%), Query Frame = 0

Query: 2   VILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALGL 61
           VI+VPPNKLA DGMVGVS L DR + TV+KVV AR CFDDTKGAVLI+NL+A+EP ALGL
Sbjct: 61  VIIVPPNKLAADGMVGVSELVDRCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGL 120

Query: 62  ETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYAQ 121
           +TYYKQ+YL LAAAAA+IKW                   IEAEKGVIVTNHSL+      
Sbjct: 121 DTYYKQHYLSLAAAAATIKW-------------------IEAEKGVIVTNHSLT------ 180

Query: 122 KILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGS 181
                  VTFNGS DH++IDATSV+NLE+I+P H+ L GTSNKKRSL+ M KTTKT GG+
Sbjct: 181 -------VTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTTKTAGGT 231

Query: 182 RLLRANLLQPLKDIETINARLDC 205
           RLLRANLLQPLKDIETIN RLDC
Sbjct: 241 RLLRANLLQPLKDIETINTRLDC 231

BLAST of Lsi09G004380 vs. ExPASy Swiss-Prot
Match: Q99MT2 (MutS protein homolog 4 OS=Mus musculus OX=10090 GN=Msh4 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 9.3e-14
Identity = 66/179 (36.87%), Postives = 89/179 (49.72%), Query Frame = 0

Query: 26  FPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALGLETYYKQYYLCLAAAAASIKWYYPN 85
           F  V    + R  F++TKG   I+ L   E S++ +E   +  Y CLAAAAA        
Sbjct: 254 FKNVNFTTVQRKYFNETKGLEYIEQLCIAEFSSVLMEV--QSRYYCLAAAAA-------- 313

Query: 86  SSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYAQKILSSGNVTFNGSSDHVSIDATSV 145
               LL Y  F+   + A K         S+K Y           F GS     ID++S 
Sbjct: 314 ----LLKYVEFIQNSVYAPK---------SLKIY-----------FQGSEQTAMIDSSSA 373

Query: 146 QNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGSRLLRANLLQPLKDIETINARLDC 205
           QNLE+   L +N    SN   +L+ +L  TKT GGSR LR+N+L+PL D+ETI+ RLDC
Sbjct: 374 QNLEL---LVNNQDYRSN--HTLFGVLNYTKTAGGSRRLRSNILEPLVDVETISMRLDC 393

BLAST of Lsi09G004380 vs. ExPASy Swiss-Prot
Match: O15457 (MutS protein homolog 4 OS=Homo sapiens OX=9606 GN=MSH4 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 3.5e-13
Identity = 64/179 (35.75%), Postives = 85/179 (47.49%), Query Frame = 0

Query: 26  FPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALGLETYYKQYYLCLAAAAASIKWYYPN 85
           F  V    + R  F++TKG   I+ L   E S + +E   K  Y CLAA AA        
Sbjct: 232 FKNVNFTTIQRKYFNETKGLEYIEQLCIAEFSTVLMEVQSK--YYCLAAVAA-------- 291

Query: 86  SSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYAQKILSSGNVTFNGSSDHVSIDATSV 145
               LL Y  F+   + A K +                     + F GS     ID++S 
Sbjct: 292 ----LLKYVEFIQNSVYAPKSL--------------------KICFQGSEQTAMIDSSSA 351

Query: 146 QNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGSRLLRANLLQPLKDIETINARLDC 205
           QNLE+   L +N    +N   +L+ +L  TKT GGSR LR+N+L+PL DIETIN RLDC
Sbjct: 352 QNLEL---LINNQDYRNN--HTLFGVLNYTKTPGGSRRLRSNILEPLVDIETINMRLDC 371

BLAST of Lsi09G004380 vs. ExPASy Swiss-Prot
Match: Q1IN52 (DNA mismatch repair protein MutS OS=Koribacter versatilis (strain Ellin345) OX=204669 GN=mutS PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 3.5e-05
Identity = 30/64 (46.88%), Postives = 41/64 (64.06%), Query Frame = 0

Query: 140 IDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGSRLLRANLLQPLKDIETIN 199
           +DA +V+NLE+IEPL +N    + +  +L+  L  T T  G RLLRA +L+P  D   IN
Sbjct: 283 LDAVTVRNLELIEPLFTN----TGEGVTLFRALDATMTPMGKRLLRAWMLRPSIDTSEIN 342

Query: 200 ARLD 204
           ARLD
Sbjct: 343 ARLD 342

BLAST of Lsi09G004380 vs. ExPASy Swiss-Prot
Match: B7GW85 (DNA mismatch repair protein MutS OS=Acinetobacter baumannii (strain AB307-0294) OX=557600 GN=mutS PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-05
Identity = 29/76 (38.16%), Postives = 46/76 (60.53%), Query Frame = 0

Query: 128 NVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGSRLLRAN 187
           ++    SSD +++D  + +NLEIIEPL  +  GT     SL+ ++   +T  G RLL   
Sbjct: 265 SILLEQSSDFIALDPITRRNLEIIEPLFEH--GT-----SLFQLVNDCQTAMGGRLLSRT 324

Query: 188 LLQPLKDIETINARLD 204
           L+QP++D   ++ARLD
Sbjct: 325 LMQPVRDTALLDARLD 333

BLAST of Lsi09G004380 vs. ExPASy TrEMBL
Match: A0A6J1DNB3 (DNA mismatch repair protein MSH4 OS=Momordica charantia OX=3673 GN=LOC111021986 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.9e-79
Identity = 166/204 (81.37%), Postives = 170/204 (83.33%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILVPPNKLAPDGMVGVSVLADRF+ TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 60  MVILVPPNKLAPDGMVGVSVLADRFYATVKKVVMARGCFDDTKGAVLIKNLAAKEPSALG 119

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSLS     
Sbjct: 120 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLS----- 179

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSL++MLKTTKTIGG
Sbjct: 180 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTTKTIGG 231

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 240 SRLLRANLLQPLKDIETINARLDC 231

BLAST of Lsi09G004380 vs. ExPASy TrEMBL
Match: A0A5A7TER1 (DNA mismatch repair protein MSH4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00610 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.8e-79
Identity = 167/204 (81.86%), Postives = 168/204 (82.35%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILV PNKLAPDGMVGVSVLADRFF TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 57  MVILVSPNKLAPDGMVGVSVLADRFFATVKKVVMARSCFDDTKGAVLIKNLAAKEPSALG 116

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSL      
Sbjct: 117 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLL----- 176

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG
Sbjct: 177 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 228

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 237 SRLLRANLLQPLKDIETINARLDC 228

BLAST of Lsi09G004380 vs. ExPASy TrEMBL
Match: A0A1S3ATN6 (DNA mismatch repair protein MSH4 OS=Cucumis melo OX=3656 GN=LOC103482597 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.8e-79
Identity = 167/204 (81.86%), Postives = 168/204 (82.35%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILV PNKLAPDGMVGVSVLADRFF TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 57  MVILVSPNKLAPDGMVGVSVLADRFFATVKKVVMARSCFDDTKGAVLIKNLAAKEPSALG 116

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSL      
Sbjct: 117 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLL----- 176

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG
Sbjct: 177 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 228

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 237 SRLLRANLLQPLKDIETINARLDC 228

BLAST of Lsi09G004380 vs. ExPASy TrEMBL
Match: A0A6J1H107 (DNA mismatch repair protein MSH4 OS=Cucurbita moschata OX=3662 GN=LOC111459167 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 5.6e-78
Identity = 163/204 (79.90%), Postives = 169/204 (82.84%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILVPPNKLAPDGMVGVSVL D+F+ TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 60  MVILVPPNKLAPDGMVGVSVLVDKFYVTVKKVVMARGCFDDTKGAVLIKNLAAKEPSALG 119

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSL+     
Sbjct: 120 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLT----- 179

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSL++MLKTTKTIGG
Sbjct: 180 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTTKTIGG 231

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 240 SRLLRANLLQPLKDIETINARLDC 231

BLAST of Lsi09G004380 vs. ExPASy TrEMBL
Match: A0A6J1K3Y6 (DNA mismatch repair protein MSH4 OS=Cucurbita maxima OX=3661 GN=LOC111491480 PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 7.2e-78
Identity = 163/204 (79.90%), Postives = 169/204 (82.84%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILVPPNKLAPDGMVGVSVL DRF+ +VKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 60  MVILVPPNKLAPDGMVGVSVLVDRFYVSVKKVVMARGCFDDTKGAVLIKNLAAKEPSALG 119

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSL+     
Sbjct: 120 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLT----- 179

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSL++MLKTTKTIGG
Sbjct: 180 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTTKTIGG 231

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 240 SRLLRANLLQPLKDIETINARLDC 231

BLAST of Lsi09G004380 vs. NCBI nr
Match: XP_038907062.1 (DNA mismatch repair protein MSH4 isoform X1 [Benincasa hispida])

HSP 1 Score: 307.8 bits (787), Expect = 7.2e-80
Identity = 167/204 (81.86%), Postives = 169/204 (82.84%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILVPPNKLAPDGMVGVSVLADRFF TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 60  MVILVPPNKLAPDGMVGVSVLADRFFATVKKVVMARACFDDTKGAVLIKNLAAKEPSALG 119

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKG+IVTNHSL      
Sbjct: 120 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGIIVTNHSLL----- 179

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG
Sbjct: 180 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 231

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 240 SRLLRANLLQPLKDIETINARLDC 231

BLAST of Lsi09G004380 vs. NCBI nr
Match: XP_038907063.1 (DNA mismatch repair protein MSH4 isoform X2 [Benincasa hispida])

HSP 1 Score: 307.8 bits (787), Expect = 7.2e-80
Identity = 167/204 (81.86%), Postives = 169/204 (82.84%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILVPPNKLAPDGMVGVSVLADRFF TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 60  MVILVPPNKLAPDGMVGVSVLADRFFATVKKVVMARACFDDTKGAVLIKNLAAKEPSALG 119

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKG+IVTNHSL      
Sbjct: 120 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGIIVTNHSLL----- 179

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG
Sbjct: 180 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 231

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 240 SRLLRANLLQPLKDIETINARLDC 231

BLAST of Lsi09G004380 vs. NCBI nr
Match: XP_022154824.1 (DNA mismatch repair protein MSH4 [Momordica charantia])

HSP 1 Score: 304.7 bits (779), Expect = 6.1e-79
Identity = 166/204 (81.37%), Postives = 170/204 (83.33%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILVPPNKLAPDGMVGVSVLADRF+ TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 60  MVILVPPNKLAPDGMVGVSVLADRFYATVKKVVMARGCFDDTKGAVLIKNLAAKEPSALG 119

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSLS     
Sbjct: 120 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLS----- 179

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSL++MLKTTKTIGG
Sbjct: 180 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLFHMLKTTKTIGG 231

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 240 SRLLRANLLQPLKDIETINARLDC 231

BLAST of Lsi09G004380 vs. NCBI nr
Match: KGN49752.2 (hypothetical protein Csa_000516 [Cucumis sativus])

HSP 1 Score: 304.3 bits (778), Expect = 7.9e-79
Identity = 167/204 (81.86%), Postives = 168/204 (82.35%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILV PNKLAPDGMVGVSVLADRFF TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 57  MVILVSPNKLAPDGMVGVSVLADRFFATVKKVVMARSCFDDTKGAVLIKNLAAKEPSALG 116

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSL      
Sbjct: 117 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLL----- 176

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG
Sbjct: 177 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 228

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 237 SRLLRANLLQPLKDIETINARLDC 228

BLAST of Lsi09G004380 vs. NCBI nr
Match: XP_008437055.1 (PREDICTED: DNA mismatch repair protein MSH4 [Cucumis melo] >KAA0039729.1 DNA mismatch repair protein MSH4 [Cucumis melo var. makuwa])

HSP 1 Score: 304.3 bits (778), Expect = 7.9e-79
Identity = 167/204 (81.86%), Postives = 168/204 (82.35%), Query Frame = 0

Query: 1   MVILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALG 60
           MVILV PNKLAPDGMVGVSVLADRFF TVKKVVMAR CFDDTKGAVLIKNL+AKEPSALG
Sbjct: 57  MVILVSPNKLAPDGMVGVSVLADRFFATVKKVVMARSCFDDTKGAVLIKNLAAKEPSALG 116

Query: 61  LETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYA 120
           LETYYKQYYLCLAAAAASIKW                   IEAEKGVIVTNHSL      
Sbjct: 117 LETYYKQYYLCLAAAAASIKW-------------------IEAEKGVIVTNHSLL----- 176

Query: 121 QKILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 180
                   VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG
Sbjct: 177 --------VTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGG 228

Query: 181 SRLLRANLLQPLKDIETINARLDC 205
           SRLLRANLLQPLKDIETINARLDC
Sbjct: 237 SRLLRANLLQPLKDIETINARLDC 228

BLAST of Lsi09G004380 vs. TAIR 10
Match: AT4G17380.1 (MUTS-like protein 4 )

HSP 1 Score: 249.6 bits (636), Expect = 2.2e-66
Identity = 136/203 (67.00%), Postives = 154/203 (75.86%), Query Frame = 0

Query: 2   VILVPPNKLAPDGMVGVSVLADRFFPTVKKVVMARVCFDDTKGAVLIKNLSAKEPSALGL 61
           VI+VPPNKLA DGMVGVS L DR + TV+KVV AR CFDDTKGAVLI+NL+A+EP ALGL
Sbjct: 61  VIIVPPNKLAADGMVGVSELVDRCYSTVRKVVFARGCFDDTKGAVLIQNLAAEEPLALGL 120

Query: 62  ETYYKQYYLCLAAAAASIKWYYPNSSLLLLWYENFLMLRIEAEKGVIVTNHSLSIKYYAQ 121
           +TYYKQ+YL LAAAAA+IKW                   IEAEKGVIVTNHSL+      
Sbjct: 121 DTYYKQHYLSLAAAAATIKW-------------------IEAEKGVIVTNHSLT------ 180

Query: 122 KILSSGNVTFNGSSDHVSIDATSVQNLEIIEPLHSNLWGTSNKKRSLYNMLKTTKTIGGS 181
                  VTFNGS DH++IDATSV+NLE+I+P H+ L GTSNKKRSL+ M KTTKT GG+
Sbjct: 181 -------VTFNGSFDHMNIDATSVENLELIDPFHNALLGTSNKKRSLFQMFKTTKTAGGT 231

Query: 182 RLLRANLLQPLKDIETINARLDC 205
           RLLRANLLQPLKDIETIN RLDC
Sbjct: 241 RLLRANLLQPLKDIETINTRLDC 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JP483.0e-6567.00DNA mismatch repair protein MSH4 OS=Arabidopsis thaliana OX=3702 GN=MSH4 PE=2 SV... [more]
Q99MT29.3e-1436.87MutS protein homolog 4 OS=Mus musculus OX=10090 GN=Msh4 PE=2 SV=1[more]
O154573.5e-1335.75MutS protein homolog 4 OS=Homo sapiens OX=9606 GN=MSH4 PE=1 SV=2[more]
Q1IN523.5e-0546.88DNA mismatch repair protein MutS OS=Koribacter versatilis (strain Ellin345) OX=2... [more]
B7GW854.6e-0538.16DNA mismatch repair protein MutS OS=Acinetobacter baumannii (strain AB307-0294) ... [more]
Match NameE-valueIdentityDescription
A0A6J1DNB32.9e-7981.37DNA mismatch repair protein MSH4 OS=Momordica charantia OX=3673 GN=LOC111021986 ... [more]
A0A5A7TER13.8e-7981.86DNA mismatch repair protein MSH4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3ATN63.8e-7981.86DNA mismatch repair protein MSH4 OS=Cucumis melo OX=3656 GN=LOC103482597 PE=4 SV... [more]
A0A6J1H1075.6e-7879.90DNA mismatch repair protein MSH4 OS=Cucurbita moschata OX=3662 GN=LOC111459167 P... [more]
A0A6J1K3Y67.2e-7879.90DNA mismatch repair protein MSH4 OS=Cucurbita maxima OX=3661 GN=LOC111491480 PE=... [more]
Match NameE-valueIdentityDescription
XP_038907062.17.2e-8081.86DNA mismatch repair protein MSH4 isoform X1 [Benincasa hispida][more]
XP_038907063.17.2e-8081.86DNA mismatch repair protein MSH4 isoform X2 [Benincasa hispida][more]
XP_022154824.16.1e-7981.37DNA mismatch repair protein MSH4 [Momordica charantia][more]
KGN49752.27.9e-7981.86hypothetical protein Csa_000516 [Cucumis sativus][more]
XP_008437055.17.9e-7981.86PREDICTED: DNA mismatch repair protein MSH4 [Cucumis melo] >KAA0039729.1 DNA mis... [more]
Match NameE-valueIdentityDescription
AT4G17380.12.2e-6667.00MUTS-like protein 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.1420.10coord: 135..204
e-value: 4.5E-19
score: 71.6
NoneNo IPR availablePANTHERPTHR11361:SF21MUTS PROTEIN HOMOLOG 4coord: 22..204
IPR007696DNA mismatch repair protein MutS, corePFAMPF05192MutS_IIIcoord: 144..204
e-value: 6.7E-15
score: 55.8
IPR045076DNA mismatch repair MutS familyPANTHERPTHR11361DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBERcoord: 22..204
IPR036187DNA mismatch repair protein MutS, core domain superfamilySUPERFAMILY48334DNA repair protein MutS, domain IIIcoord: 141..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G004380.1Lsi09G004380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007129 homologous chromosome pairing at meiosis
biological_process GO:0010777 meiotic mismatch repair involved in reciprocal meiotic recombination
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0006298 mismatch repair
cellular_component GO:0000794 condensed nuclear chromosome
cellular_component GO:0043073 germ cell nucleus
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030983 mismatched DNA binding
molecular_function GO:0022857 transmembrane transporter activity