Lsi09G003880 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G003880
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	chr09: 4094876 .. 4095484 (+)
RNA-Seq Expression	Lsi09G003880
Synteny	Lsi09G003880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTCTCCTCCCTCCAAATTAGAAATGGCAAATCTCAAGATCTCACTACTCCTCATTATCCTCGTGGCTCTCCACAATGCGGCAACCGCAGAGTCGGCAGCGACAAGCTTCATCGAGTCATCCTGCAGAGTGACCCGCTACCCGGCGCTGTGCGTTCAGTCCTTGTCAAGCTACGCAACCACCATCCGCCAGAGCGGTCGTCAGCTGGCCAGAATGGCCCTGTCCGTGAGCCTGAGCAAGGCCCGTCTCGCCGCAGCCTTCGTGGGCAAACTGGGCAAAGGCGGTGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGACTGCATAGAGAATATGGGGGACAGCGTGGACAGGCTCAGCCAATCGGTGAAGGAGCTCGGGGACTTACGTCGAACAGCTGGTGGGGACTTCTTATGGCACATGAATAATGTCCAGACTTGGGTCAGCGCCGCCCTCACTGATGAAACCACTTGCCTTGATGGCTTTGCTGGCCGCCGCTTAGACGGAAAAGTCAAGGCTACGATTCGCCGGAGAATTACCCTTGTTGCTCAAATCACTAGTAATGCTCTAGCTCTTGTTAATCGCTTTGCAGATAAACACCACTGA

mRNA sequence

ATGCTTTCTCCTCCCTCCAAATTAGAAATGGCAAATCTCAAGATCTCACTACTCCTCATTATCCTCGTGGCTCTCCACAATGCGGCAACCGCAGAGTCGGCAGCGACAAGCTTCATCGAGTCATCCTGCAGAGTGACCCGCTACCCGGCGCTGTGCGTTCAGTCCTTGTCAAGCTACGCAACCACCATCCGCCAGAGCGGTCGTCAGCTGGCCAGAATGGCCCTGTCCGTGAGCCTGAGCAAGGCCCGTCTCGCCGCAGCCTTCGTGGGCAAACTGGGCAAAGGCGGTGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGACTGCATAGAGAATATGGGGGACAGCGTGGACAGGCTCAGCCAATCGGTGAAGGAGCTCGGGGACTTACGTCGAACAGCTGGTGGGGACTTCTTATGGCACATGAATAATGTCCAGACTTGGGTCAGCGCCGCCCTCACTGATGAAACCACTTGCCTTGATGGCTTTGCTGGCCGCCGCTTAGACGGAAAAGTCAAGGCTACGATTCGCCGGAGAATTACCCTTGTTGCTCAAATCACTAGTAATGCTCTAGCTCTTGTTAATCGCTTTGCAGATAAACACCACTGA

Coding sequence (CDS)

ATGCTTTCTCCTCCCTCCAAATTAGAAATGGCAAATCTCAAGATCTCACTACTCCTCATTATCCTCGTGGCTCTCCACAATGCGGCAACCGCAGAGTCGGCAGCGACAAGCTTCATCGAGTCATCCTGCAGAGTGACCCGCTACCCGGCGCTGTGCGTTCAGTCCTTGTCAAGCTACGCAACCACCATCCGCCAGAGCGGTCGTCAGCTGGCCAGAATGGCCCTGTCCGTGAGCCTGAGCAAGGCCCGTCTCGCCGCAGCCTTCGTGGGCAAACTGGGCAAAGGCGGTGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGACTGCATAGAGAATATGGGGGACAGCGTGGACAGGCTCAGCCAATCGGTGAAGGAGCTCGGGGACTTACGTCGAACAGCTGGTGGGGACTTCTTATGGCACATGAATAATGTCCAGACTTGGGTCAGCGCCGCCCTCACTGATGAAACCACTTGCCTTGATGGCTTTGCTGGCCGCCGCTTAGACGGAAAAGTCAAGGCTACGATTCGCCGGAGAATTACCCTTGTTGCTCAAATCACTAGTAATGCTCTAGCTCTTGTTAATCGCTTTGCAGATAAACACCACTGA

Protein sequence

MLSPPSKLEMANLKISLLLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITSNALALVNRFADKHH

Homology

[Close]

BLAST of Lsi09G003880 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 4.8e-47
Identity = 98/195 (50.26%), Postives = 139/195 (71.28%), Query Frame = 0


Query: 7   KLEMANLKISLLLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQS 66
           KL  + L  S LL+IL     AA + + A  FI++SC +T YPA+C QSLS+YA TI+ +
Sbjct: 2   KLSKSTLVFSALLVIL-----AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNN 61

Query: 67  GRQLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKE 126
            ++LA  AL VSL++ + A  F+ +L K  G+K  +Y A+ DC+E + DS+DR+S+S  E
Sbjct: 62  PQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDE 121

Query: 127 LGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQI 186
           + +L    G DF + M+NV+TWVSAALTDETTC+DGFAG+ +DGK+K ++R ++  VA++
Sbjct: 122 MKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARV 181

Query: 187 TSNALALVNRFADKH 202
           TSNALALVN FA KH
Sbjct: 182 TSNALALVNNFAAKH 191

[Close]

BLAST of Lsi09G003880 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-44
Identity = 106/196 (54.08%), Postives = 138/196 (70.41%), Query Frame = 0


Query: 13  LKISLLLIILVAL-----HNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSG 72
           LK ++ L+IL+++      +A    S +  FI SSC+ T+YP+LCV +LS+YAT IR + 
Sbjct: 3   LKNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNN 62

Query: 73  RQ-LARMALSVSLSKARLAAAFVGKLGK-GGGMKGLEYQAVKDCIENMGDSVDRLSQSVK 132
            Q LA+ AL +SL++A+    FV KL K     K  EY A+KDCIE +G+SVDRL+QSVK
Sbjct: 63  DQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVK 122

Query: 133 ELGDL-RRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVA 192
           EL       A  DF+W M+NVQTWVSAALTDETTCLDGF+ R + GKVK  IR ++  VA
Sbjct: 123 ELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVA 182

Query: 193 QITSNALALVNRFADK 201
           Q+TSNALALVN+FA+K
Sbjct: 183 QVTSNALALVNQFAEK 198

[Close]

BLAST of Lsi09G003880 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 8.8e-41
Identity = 93/198 (46.97%), Postives = 131/198 (66.16%), Query Frame = 0


Query: 12  NLKISLLLIIL-------VALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIR 71
           N ++SL+L +L       V   N     S  T FI++SC  T YP  C QSLSSYA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 72  QSGRQLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSV 131
           +  R+LA  AL+VS+++A+ A  +V ++    G+   +++AV DC+E MGD+VDRLS S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 132 KELGDLRR-TAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLV 191
           KEL  L    +G DF + ++NV+TW SAALTDET C+DGF G+ + G++K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 192 AQITSNALALVNRFADKH 202
           A+ TSNALAL+N FA KH
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]

BLAST of Lsi09G003880 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.7e-39
Identity = 86/175 (49.14%), Postives = 119/175 (68.00%), Query Frame = 0


Query: 28  AATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSVSLSKARLAAA 87
           AA     A +FI++SC+ T YP +CV SL+ YA +I+ S R+LA  AL+V++++A+    
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 88  FVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGDFLW-HMNNVQ 147
           FV +LG+   +K  E QAVKDCIE + D+VDRL+ S+ E+       G D  W HM+N Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 148 TWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITSNALALVNRFADKH 202
           TW SAALT+  TC DGFAGR +DG+VK ++R RI  + + TSNALAL+N FA K+
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKKY 202

[Close]

BLAST of Lsi09G003880 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.4e-32
Identity = 73/161 (45.34%), Postives = 109/161 (67.70%), Query Frame = 0


Query: 38  FIESSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSVSLSKARLAAAFVGKLGKGGG 97
           +I++SC +T Y  +C  SLS YA+TIR + ++LA +AL+++LS A+ A+ FV  +  GGG
Sbjct: 147 YIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKFVKNISHGGG 206

Query: 98  MKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDET 157
           +  LE  AV DC+E +GDSV  L  S++EL  +       F   M++V+TWVSAALT++ 
Sbjct: 207 LTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETWVSAALTNDD 266

Query: 158 TCLDGFAGRRLDGKVKATIRRRITLVAQITSNALALVNRFA 199
           TC+DGF+   +   VK  +RR +  VA++TSNALAL+N +A
Sbjct: 267 TCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of Lsi09G003880 vs. ExPASy TrEMBL
Match: A0A5A7T9V3 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00090 PE=4 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.8e-87
Identity = 176/194 (90.72%), Postives = 183/194 (94.33%), Query Frame = 0


Query: 10  MANLKISL-LLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           MANLKISL LLI L+ALHNAAT  S +TSFIESSC+VTRYPALCVQSLS+YA TIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QLAR ALSVSLSKARLAAAFV KLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG+ KA IRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 190 NALALVNRFADKHH 203
           NALALVNRFAD++H
Sbjct: 181 NALALVNRFADENH 194

[Close]

BLAST of Lsi09G003880 vs. ExPASy TrEMBL
Match: A0A0A0KR60 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 6.5e-87
Identity = 175/194 (90.21%), Postives = 184/194 (94.85%), Query Frame = 0


Query: 10  MANLKISL-LLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           MANLKISL LLI LVALHNAAT  SAATSFIESSC+VTRYPALCVQSLS+YA  IRQSGR
Sbjct: 1   MANLKISLPLLITLVALHNAATTGSAATSFIESSCKVTRYPALCVQSLSTYANVIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QLAR ALSVSLSKARLA+AFV KLGKGGGMKGLEYQAVKDCIENMGD+VDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKELG 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLR+TAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG++KA IRRRITLVAQITS
Sbjct: 121 DLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQITS 180

Query: 190 NALALVNRFADKHH 203
           NALALVNRFAD++H
Sbjct: 181 NALALVNRFADENH 194

[Close]

BLAST of Lsi09G003880 vs. ExPASy TrEMBL
Match: A0A1S3ATE5 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.5e-86
Identity = 175/194 (90.21%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 10  MANLKISL-LLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           MANLKISL LLI L+ALHNAAT  S +TSFIESSC+VTRYPALCVQSLS+YA TIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QLAR ALSVSLSKA LAAAFV KLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG+ KA IRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 190 NALALVNRFADKHH 203
           NALALVNRFAD++H
Sbjct: 181 NALALVNRFADENH 194

[Close]

BLAST of Lsi09G003880 vs. ExPASy TrEMBL
Match: A0A6J1H2L8 (pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 3.6e-77
Identity = 156/194 (80.41%), Postives = 172/194 (88.66%), Query Frame = 0


Query: 10  MANLKISLLLIILVALHNAATAESA-ATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           M NLKISL L++LV LHNAA   SA ATSFIESSCRVTRYPALCV SLS+YAT+IRQS R
Sbjct: 1   MPNLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSSR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QL + ALSVSL+K+RLAAAFV  L KGG ++GLEYQA+KDCIENMGD+VDRL QSVKEL 
Sbjct: 61  QLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRLRQSVKELR 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLRRTAGGD LWH++NVQTWVSAALTDETTCLDGFA RRLDG+VKA IRR+ITLVAQ+TS
Sbjct: 121 DLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAVIRRKITLVAQVTS 180

Query: 190 NALALVNRFADKHH 203
           NALALVN FADK H
Sbjct: 181 NALALVNHFADKKH 194

[Close]

BLAST of Lsi09G003880 vs. ExPASy TrEMBL
Match: A0A6J1K807 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491486 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.3e-76
Identity = 155/196 (79.08%), Postives = 172/196 (87.76%), Query Frame = 0


Query: 8   LEMANLKISLLLIILVALHNAATAESA-ATSFIESSCRVTRYPALCVQSLSSYATTIRQS 67
           + M NLKISL L++LV LHNAA   SA ATSFIESSCRVTRYPALCV SLS+YAT+IRQS
Sbjct: 20  IAMPNLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQS 79

Query: 68  GRQLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKE 127
           GRQL + ALSVSL+K+R AAAFV  L KG  ++GLEYQA+KDCIENMGD+VDRL QSVKE
Sbjct: 80  GRQLTQTALSVSLNKSRYAAAFVSNLAKGRAVRGLEYQALKDCIENMGDTVDRLRQSVKE 139

Query: 128 LGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQI 187
           L DLRRTAGGD LWH++NVQTWVSAALTDETTCLDGFA RRLDG+VKA IRR+ITLVAQ+
Sbjct: 140 LRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQV 199

Query: 188 TSNALALVNRFADKHH 203
           TSNALALVN FADK H
Sbjct: 200 TSNALALVNHFADKKH 215

[Close]

BLAST of Lsi09G003880 vs. NCBI nr
Match: XP_038875547.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 356.7 bits (914), Expect = 1.3e-94
Identity = 191/204 (93.63%), Postives = 198/204 (97.06%), Query Frame = 0


Query: 1   MLSPPSKLEMANLKISL-LLIILVALHNAATAE-SAATSFIESSCRVTRYPALCVQSLSS 60
           +LSPPSKL MANLKISL +LIILVALHNAATAE SAATSFIESSC+VTRYPALCVQSLSS
Sbjct: 4   ILSPPSKLAMANLKISLPILIILVALHNAATAESSAATSFIESSCKVTRYPALCVQSLSS 63

Query: 61  YATTIRQSGRQLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVD 120
           YATTIRQSGRQLARMALSVSLSKARLAAAFV KLGKGGGMKGLEYQAVKDCIENMGDSVD
Sbjct: 64  YATTIRQSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVD 123

Query: 121 RLSQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRR 180
           RL QSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG++KA IR+
Sbjct: 124 RLGQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAAIRQ 183

Query: 181 RITLVAQITSNALALVNRFADKHH 203
           RITLVAQITSNALALVNRFADK+H
Sbjct: 184 RITLVAQITSNALALVNRFADKNH 207

[Close]

BLAST of Lsi09G003880 vs. NCBI nr
Match: KAA0039678.1 (21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 330.9 bits (847), Expect = 7.8e-87
Identity = 176/194 (90.72%), Postives = 183/194 (94.33%), Query Frame = 0


Query: 10  MANLKISL-LLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           MANLKISL LLI L+ALHNAAT  S +TSFIESSC+VTRYPALCVQSLS+YA TIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QLAR ALSVSLSKARLAAAFV KLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG+ KA IRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 190 NALALVNRFADKHH 203
           NALALVNRFAD++H
Sbjct: 181 NALALVNRFADENH 194

[Close]

BLAST of Lsi09G003880 vs. NCBI nr
Match: XP_004147584.1 (21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 1.3e-86
Identity = 175/194 (90.21%), Postives = 184/194 (94.85%), Query Frame = 0


Query: 10  MANLKISL-LLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           MANLKISL LLI LVALHNAAT  SAATSFIESSC+VTRYPALCVQSLS+YA  IRQSGR
Sbjct: 1   MANLKISLPLLITLVALHNAATTGSAATSFIESSCKVTRYPALCVQSLSTYANVIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QLAR ALSVSLSKARLA+AFV KLGKGGGMKGLEYQAVKDCIENMGD+VDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKELG 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLR+TAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG++KA IRRRITLVAQITS
Sbjct: 121 DLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQITS 180

Query: 190 NALALVNRFADKHH 203
           NALALVNRFAD++H
Sbjct: 181 NALALVNRFADENH 194

[Close]

BLAST of Lsi09G003880 vs. NCBI nr
Match: XP_008437134.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 328.2 bits (840), Expect = 5.1e-86
Identity = 175/194 (90.21%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 10  MANLKISL-LLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           MANLKISL LLI L+ALHNAAT  S +TSFIESSC+VTRYPALCVQSLS+YA TIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QLAR ALSVSLSKA LAAAFV KLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDG+ KA IRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 190 NALALVNRFADKHH 203
           NALALVNRFAD++H
Sbjct: 181 NALALVNRFADENH 194

[Close]

BLAST of Lsi09G003880 vs. NCBI nr
Match: KAG6606163.1 (Pectinesterase inhibitor 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 300.8 bits (769), Expect = 8.7e-78
Identity = 157/194 (80.93%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 10  MANLKISLLLIILVALHNAATAESA-ATSFIESSCRVTRYPALCVQSLSSYATTIRQSGR 69
           M NLKISL L++LV LHNAA   SA ATSFIESSCRVTRYPALCV SLS+YAT+IRQSGR
Sbjct: 1   MPNLKISLPLLVLVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSGR 60

Query: 70  QLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 129
           QL + ALSVSL+K+RLAAAFV  L KGG ++GLEYQA+KDCIENMGD+VDRL QSVKEL 
Sbjct: 61  QLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRLRQSVKELR 120

Query: 130 DLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 189
           DLRRTAGGD LWH++NVQTWVSAALTDETTCLDGFA RRLDG+VKA IRR+ITLVAQ+TS
Sbjct: 121 DLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVTS 180

Query: 190 NALALVNRFADKHH 203
           NALALVN FADK+H
Sbjct: 181 NALALVNHFADKNH 194

[Close]

BLAST of Lsi09G003880 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 183.0 bits (463), Expect = 2.5e-46
Identity = 98/191 (51.31%), Postives = 139/191 (72.77%), Query Frame = 0


Query: 13  LKISLLLIILVALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGRQLAR 72
           L + LL    +A  +++   S++ +FI SSCRVTRY  LCV+ L+++A  IR++  QLA+
Sbjct: 14  LFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQLAQ 73

Query: 73  MALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRR 132
            AL+V+L + +    +VGKL K   +K  EY AVKDC+EN+GD ++ L+QS++EL  + R
Sbjct: 74  TALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQVGR 133

Query: 133 TA--GGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITSNA 192
           +     +FLW ++NV+TWVSAALTDETTCLDGF G+ +DG VK+ IRRR+  VA++TSNA
Sbjct: 134 SGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNA 193

Query: 193 LALVNRFADKH 202
           LALVNRFA +H
Sbjct: 194 LALVNRFAARH 204

[Close]

BLAST of Lsi09G003880 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 180.3 bits (456), Expect = 1.6e-45
Identity = 106/196 (54.08%), Postives = 138/196 (70.41%), Query Frame = 0


Query: 13  LKISLLLIILVAL-----HNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSG 72
           LK ++ L+IL+++      +A    S +  FI SSC+ T+YP+LCV +LS+YAT IR + 
Sbjct: 3   LKNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNN 62

Query: 73  RQ-LARMALSVSLSKARLAAAFVGKLGK-GGGMKGLEYQAVKDCIENMGDSVDRLSQSVK 132
            Q LA+ AL +SL++A+    FV KL K     K  EY A+KDCIE +G+SVDRL+QSVK
Sbjct: 63  DQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVK 122

Query: 133 ELGDL-RRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVA 192
           EL       A  DF+W M+NVQTWVSAALTDETTCLDGF+ R + GKVK  IR ++  VA
Sbjct: 123 ELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVA 182

Query: 193 QITSNALALVNRFADK 201
           Q+TSNALALVN+FA+K
Sbjct: 183 QVTSNALALVNQFAEK 198

[Close]

BLAST of Lsi09G003880 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 6.3e-42
Identity = 93/198 (46.97%), Postives = 131/198 (66.16%), Query Frame = 0


Query: 12  NLKISLLLIIL-------VALHNAATAESAATSFIESSCRVTRYPALCVQSLSSYATTIR 71
           N ++SL+L +L       V   N     S  T FI++SC  T YP  C QSLSSYA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 72  QSGRQLARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSV 131
           +  R+LA  AL+VS+++A+ A  +V ++    G+   +++AV DC+E MGD+VDRLS S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 132 KELGDLRR-TAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLV 191
           KEL  L    +G DF + ++NV+TW SAALTDET C+DGF G+ + G++K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 192 AQITSNALALVNRFADKH 202
           A+ TSNALAL+N FA KH
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]

BLAST of Lsi09G003880 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 4.1e-41
Identity = 94/190 (49.47%), Postives = 128/190 (67.37%), Query Frame = 0


Query: 13  LKISLLLIILVALHNAAT---AESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGRQ 72
           L IS LL + +    AA+   A   A +FI+SSC+ T YPALCV SLS YA  I+ S ++
Sbjct: 10  LSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKR 69

Query: 73  LARMALSVSLSKARLAAAFVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGD 132
           LA  A++V+LS+A+    FV +L +  G+K  E +A+KDC+E M D+VDRL++SV+EL  
Sbjct: 70  LAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKL 129

Query: 133 LRRTAGGD-FLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITS 192
                  D F +HM+N QTW SAALTDE TC DGF+GR +DG++K ++R RI  V   TS
Sbjct: 130 CGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETS 189

Query: 193 NALALVNRFA 199
           NAL+L+N FA
Sbjct: 190 NALSLINAFA 199

[Close]

BLAST of Lsi09G003880 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.6e-40
Identity = 86/175 (49.14%), Postives = 119/175 (68.00%), Query Frame = 0


Query: 28  AATAESAATSFIESSCRVTRYPALCVQSLSSYATTIRQSGRQLARMALSVSLSKARLAAA 87
           AA     A +FI++SC+ T YP +CV SL+ YA +I+ S R+LA  AL+V++++A+    
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 88  FVGKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGGDFLW-HMNNVQ 147
           FV +LG+   +K  E QAVKDCIE + D+VDRL+ S+ E+       G D  W HM+N Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 148 TWVSAALTDETTCLDGFAGRRLDGKVKATIRRRITLVAQITSNALALVNRFADKH 202
           TW SAALT+  TC DGFAGR +DG+VK ++R RI  + + TSNALAL+N FA K+
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAKKY 202

The following BLAST results are available for this feature:

BLAST of Lsi09G003880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	4.8e-47	50.26	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI72	2.2e-44	54.08	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	8.8e-41	46.97	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9STY5	3.7e-39	49.14	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI74	3.4e-32	45.34	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Lsi09G003880 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T9V3	3.8e-87	90.72	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G...	[more]
A0A0A0KR60	6.5e-87	90.21	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 S...	[more]
A0A1S3ATE5	2.5e-86	90.21	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1	[more]
A0A6J1H2L8	3.6e-77	80.41	pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE...	[more]
A0A6J1K807	2.3e-76	79.08	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491486 PE=4 SV=1	[more]

BLAST of Lsi09G003880 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875547.1	1.3e-94	93.63	21 kDa protein-like [Benincasa hispida]	[more]
KAA0039678.1	7.8e-87	90.72	21 kDa protein-like [Cucumis melo var. makuwa]	[more]
XP_004147584.1	1.3e-86	90.21	21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cu...	[more]
XP_008437134.1	5.1e-86	90.21	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
KAG6606163.1	8.7e-78	80.93	Pectinesterase inhibitor 9, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi09G003880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12390.1	2.5e-46	51.31	pectin methylesterase inhibitor 1	[more]
AT1G62770.1	1.6e-45	54.08	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	6.3e-42	46.97	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	4.1e-41	49.47	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	2.6e-40	49.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 34..193 e-value: 1.5E-53 score: 193.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 37..193 e-value: 1.5E-40 score: 139.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 11..195 e-value: 1.6E-37 score: 126.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 32..197 e-value: 4.4E-41 score: 142.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 37..197
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 19..201
None	No IPR available	PANTHER	PTHR31080:SF207	PECTINESTERASE INHIBITOR 9	coord: 19..201
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 41..198 e-value: 1.01852E-47 score: 151.822

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G003880.1	Lsi09G003880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity