Lsi09G003830 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G003830
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncytochrome P450, family 706, subfamily A, polypeptide 3
Locationchr09: 4037140 .. 4042335 (-)
RNA-Seq ExpressionLsi09G003830
SyntenyLsi09G003830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTACACTCGTTTACCTATTTCAAATTCATAGAAATGTGTAGATATCTAGGAAACCACAACATGTTTGTTTGTTCTTTTTTTTTTTTCCTAGAAAAATACTATAAAGGTTATTAATTATTGTTTGTTTGTTATTTTTTTTTCCTAGAAAAATACTATAAAGGTTATTAATTATTCAGAATGGTTTCAAGATATATTTGAGCTTCAAATTATTCAGAAGGTTATCAACTGTTCCTCCAAGTGGTTGACATGTCAATCTCAATTTCCTTCCTCTTCTCATTTTGGTCCGTAGATTCCAACGAAGACGAACAAATTTCAACCGCCCTTCTTTTTATCTTCGTCTTCGTTGTTGCTCTGTTATGGCTCCGACCCAAATTCCGGCGTCCCTCTTTGCCTCCAGGCCCCCGTGGCCTGCCGCTGGTCGGATACCTTCCCTTTTTATCCCGCAATCTCCACCATACGTTCGCCAATTTGGCCGAAATCTACGGCCCAATTTTCAAGCTCCGCCTTGGAACCAAGCTCTGCGTCGTTCTCACCTCCCCTTCCTCCATCAATGAAGCCCTCCGCCACCAAGAAACCGTCTTCGCCAACCGAGATTCCACCGTCAGTGCTCTTCTCGCCACCTACGGCGGAGTCGATATTGTGTTCAGTCAGGACGACGGCGATTGGAAGAAGCTAAGAAAAATCTTCGCCCGGAAAATGCTTAGCAAATCAAATCTGGATGCGTCCTATCCTTTGCGAAGACAAGAAGTAAGAAAAGTGATTAAAGGCGTGTTCGAATCGGGGGGAAACCCAGTTGATATCGGGAAACTGGGTTTCTTCGCCACTGTGAAATCTGTTATGGCAATGACGTGGGGTGGGTCGGGGGGACTGATCGGAGCGGATGGGACTGATTTGGAAGCTAAGTTCAGGGAAGTGGTGGATGAACTGATGGTGTTGGTTGGATCTCCGAATTTGTCGGATCTTTTTCCTGTGTTGGGTCGGTTTGATTTGCAGGGAATTGCGAGGAAGATGAAGAAGGTGATGAATGTTTGTGATGAGATTCTGAATTCCGCCATTGAAGAACAGAGGAAGGTGGGAGAAAATGGTGCGGAAAGGAGAGGATTTTTGCAGTTTCTGTTGGAGGTTAAGGACAGCAAAGATAGTTCAGAGTCCATTACAGATAACCAACTGAAGGCCTTGCTAATGGTATGACTTTTTATTTATTTATTTAGGCGAACAAAGAATTAGTAGATTAAAGATCTGCCAAATCTTCGGAGAAAATTTCGTAAACTAACCAATCTGAAAAATTAGAAAACCAACAATAGGAGACGGCAAAAAAACCTCGACCAGCGAAGATATGAGCCGTCCGATTCCAATGGTTGCTAATGCTATCCTTTCTTAATTAATTATTATTATTTCATTCCCAAATGGAAATTCTTCACTTAGAAATTTAGAATATGCATTTTTTTCTTCTTAAATGATCAATAAATGCTTTTTAAATGGCAACCACTTGAATCTAAAGGATTAGTTAACAATAAATGGCATTGATTCTTTGATAAGAAAATTCAACTTAAAAAAAAAAAAGAAAAAGAAAAAAAAAAAAAGGTCATATTAAGGTGTTTAGTAACTATTTGGATTTATAGAAAAAAAATTATATTTGTTTTCTACTATTTTTAAGATTCTGATCATAGTTTTTAAATAAATATTTGAATTCTTATCCAAAATTTAAAAACAGTAACATATTTTCACAAAATACTTTTCTAACTTTAAAAAATTGACATGAATTTTTAGAACACTCCTATATATAGACAACAAAACAAAAAAATCATCCGTAGACCAAGTGAGCTTAACTCAACTAATTGACATGACATTCCTCCTTAGAGATTAGAGGTTTGATCTCATCCTCACAATTGCTATACTAAAAAAACTAAAAACTAAGGTATTGTTTGGAAACTATTTGGTTTTTTAAAATTGTGTTTGTTTTTTCACAATTTCCTATGTAAACATTTGAATTCATAACCAATTTCTAAAAACAAAAACTATTTTTAAAAGCTATTTTTTTGTTTTTAAAATTTAATTTTGATTTTGAAACCACTCCTAAAATGTTGACTACATAGAAGCCGGTAGATGAAAGTTATGTTTTAATTTTTAAAGTCAGAAAACCTAAAAACAAATGATTACTGTATAGAATCTAAATAACATAAAATTAAATGGTATTTAAAAGAATGGGAAAGTAAAAATTAGCCAACACATGGCACCCTCTAGTTGACAAGGTGTATTGATTGCGAAAAATGGCTTTTGCAGCCAAGTTTGCTCATAATATATTGGTGAAATTATAAAGAATGCCTTTAAAATTTTATATTTTGTATTAAAAATATCACTAAACTTTTAAAAATTTCAAAAATATCCTTGAATTTTTAAAAATAGTTAAAAAAATATCCTTACAGTTAGTTTTAGATAGAAACAACTAATGTTTTATTTCAAAAATACTCCTGAACTTCTAAAAGTTTTAAAAATATTCTTAAACTTAAAAAAAAAAAATACTCTTTCTGTTAGTATATAAACAAAAACCGTTAATATGTCATTGCAAAAATAACTCTAAATTTTCAAAAGTTGCCTTAGTACCCTTACCGTTTAAAAAGAAAAAAAAAATTTACCGTTTATCTACACTTCATCACCTCCCTCTTCAATTTCCTCTTCTTCTCTATTTCAACCTATGATCTAACACACTTCTCTATTTTCTCTCTTTTTTCAATACCCTCCTATTCTCCCTCTTGTCGACTTTTTTACCGTTGATTATAACAAGAAGAAAAATAATAATGATCAAACTAGAGATGTCCACTTTTCCAATGAGAGTGGACCCTGCAGGGACCCGCCTTGAAGAGGATGGGGAATGGGAGAGGGAGTGGAGGAAATTTTTTTTCCAAAAGCTAAATAGAGACGGAAATAGAGAATGCATTCCTCGCCCCCGTCACGTTAGACGTTTTTTCTTATATTTATTATAATAATTGGATGATATGTTATTTATTTTTATTATATAAATATTACATTTAATTTTAAGATTTATTGTTTAATGGTCAAAATAATGAATATGTTTGAATTAGAGTAAAAAAAAAGATATTTTTGAATTCTAATAAATCTGATAGATAATTATTTCCTTCAACTTAAAAAAACTGAGTAATTTCTTTTTAAATTTTAATTGTCCATGAGGTATTAATCATTTAATTGATTTTTTTAATGACAAAACAAATGGAAAAAAATTCTCCGCGTGGAACTCGATCCCCACGGGGAATTCCCGTCCCTCTCCATGCGAAATTTGGCGGGGATGGAGAATGAAATGGGGAGAGAGGACGGGGAGCGGGGAAGTCATCCTCGACCCCACCCTGCCCCATGAACATCTCTAAGTGGAACCACAAAAAATCTTGAGGACTTAAATGTTTTAACAAAATTATACTTTAGCATTTGAGTATGTGTTAATAGCCAAATACATTCCTTTTGTATTTGACTATTTATCATTTTGGTGAGTTTTAGGGGAAGATTTTGATTTTGAGATTTTATGAGATTTTTTACTTTAATTTTAAGTTTTGGTTTATATTTGGTTGCTAAGAAAATTGATGTAAGCTTGGGCAAATGAGATAAAATGAGAGTATTTATGTGAAAAAAAATTGATAGAATCTATATAGTTTGTTTTAAAATTGATTATTTTCTAAGTCAAGATAACTCAAATAAATTGATCATTATACTAATGGAAGGAGCATTTTTGAACCTTTTTTTTTTAAAAGACTTAAGCATTAAGAACATTTTAAAATTCGGGCATATTTTTTTTAAACGAGATATTAACAGTTTTCTTCATATATTAAGGATAAGGGTATTTTTTTAATTTTTTTTTTTTTAAAGTTTAGGGATATTATTGAAAATTTTGAAACTAAGTACTAACGTTGTTTCTGTCCAAAGATAATGATAAAAGTATTTTTGAACTCTTTTTTAAAAGTTTAACGATATTTTTTGAACTTTTAAAAGTTAAGAGATATTTTTGACTCAAAGTATAAAGTTTGGTATTTTTTATAATTTAGCCTAATATATTTATACCTTGACTATGACAATTTCTAAATTTTTGCCCAATCATTTTTTCTCATAGTGGAATCTCCAATATGGCAGTTTTTAATTCATAGTGGAATCTCCATTATGCAGTTTTTAATTACTAATACTTTCTAAAAATCATTTCTTTGGATAAACTAAAACATACAAGCCCTTAAGCAATTAGTGTCTAGAGTGTTCCTAAATTTCAAAAAAAAAAAATTTGCATTTTACTTTTTGGATTGAATTTATTATTTATATAGTACGTCTTTATGCCTAAGAACACTCCTCTTTTATATTAGTTTTGTATGATTTTGAAAATAGGACATCATCGTTGGAGGAACAGACTCGACATCAACTACAATCGAATGGACAATAACAGAGTTAATACAACAACCAAATACAATGAAGAAAGTCGTGGAAGAATTGACAAAAGTTGTAGGATTAAACCAAATGGTTGAAGAATTTCACTTATCTAAACTACATTATTTAGATGCGGTGATTAAAGAGACACTTCGTTTACACCCACCCTTACCTCTTTTAGTGCCACGTACACCTAGCCAAACAAGCACCCTTGGAGGGTACACTATCCCAAAAGGCTCAGCTATCTACTTCAACATTTGGGCCATACAGAGAGATCCTAAAGTTTGGGACAAGCCCTTAGACTTTATGCCCGAAAGATTCTTGAATGAATCTACTGAAAAAGTGTATGATTTCACTGGCAATAACATAGAGTTCTGTCCATTTGGGTCAGGTAAAAAGTTATGTGCAGGGATTCCTCTAGCTGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGCTTTTGAGTGGAAATTGTCTGAAGGTTCAAAGCTTGATTTAGAAGAGAAGTTTGGAATTGTCACCAAGAAGTTGAACCCGTTGGTTGTTATTCCTATGCCAAGGCTTTCCAACTTGGAGCTTTATAATATTACGTAGATTTGGTCTAAATAATTGCCAATATTGGCATCATTTCACTACTCAAATCAAGGATTCCTGTGTTTGTAATATAATGAGGCAACTAAGATGACATATATTCTATGAATGGATTGGCATAAAATAATTTTAGGAGTTTGAACAACCTAATTTGGGGAATCAAAATTTACCTTAAACTTT

mRNA sequence

CTACACTCGTTTACCTATTTCAAATTCATAGAAATGTGTAGATATCTAGGAAACCACAACATGTTTGTTTGTTCTTTTTTTTTTTTCCTAGAAAAATACTATAAAGGTTATTAATTATTCAGAATGGTTTCAAGATATATTTGAGCTTCAAATTATTCAGAAGGTTATCAACTGTTCCTCCAAGTGGTTGACATGTCAATCTCAATTTCCTTCCTCTTCTCATTTTGGTCCGTAGATTCCAACGAAGACGAACAAATTTCAACCGCCCTTCTTTTTATCTTCGTCTTCGTTGTTGCTCTGTTATGGCTCCGACCCAAATTCCGGCGTCCCTCTTTGCCTCCAGGCCCCCGTGGCCTGCCGCTGGTCGGATACCTTCCCTTTTTATCCCGCAATCTCCACCATACGTTCGCCAATTTGGCCGAAATCTACGGCCCAATTTTCAAGCTCCGCCTTGGAACCAAGCTCTGCGTCGTTCTCACCTCCCCTTCCTCCATCAATGAAGCCCTCCGCCACCAAGAAACCGTCTTCGCCAACCGAGATTCCACCGTCAGTGCTCTTCTCGCCACCTACGGCGGAGTCGATATTGTGTTCAGTCAGGACGACGGCGATTGGAAGAAGCTAAGAAAAATCTTCGCCCGGAAAATGCTTAGCAAATCAAATCTGGATGCGTCCTATCCTTTGCGAAGACAAGAAGTAAGAAAAGTGATTAAAGGCGTGTTCGAATCGGGGGGAAACCCAGTTGATATCGGGAAACTGGGTTTCTTCGCCACTGTGAAATCTGTTATGGCAATGACGTGGGGTGGGTCGGGGGGACTGATCGGAGCGGATGGGACTGATTTGGAAGCTAAGTTCAGGGAAGTGGTGGATGAACTGATGGTGTTGGTTGGATCTCCGAATTTGTCGGATCTTTTTCCTGTGTTGGGTCGGTTTGATTTGCAGGGAATTGCGAGGAAGATGAAGAAGGTGATGAATGTTTGTGATGAGATTCTGAATTCCGCCATTGAAGAACAGAGGAAGGTGGGAGAAAATGGTGCGGAAAGGAGAGGATTTTTGCAGTTTCTGTTGGAGGTTAAGGACAGCAAAGATAGTTCAGAGTCCATTACAGATAACCAACTGAAGGCCTTGCTAATGGACATCATCGTTGGAGGAACAGACTCGACATCAACTACAATCGAATGGACAATAACAGAGTTAATACAACAACCAAATACAATGAAGAAAGTCGTGGAAGAATTGACAAAAGTTGTAGGATTAAACCAAATGGTTGAAGAATTTCACTTATCTAAACTACATTATTTAGATGCGGTGATTAAAGAGACACTTCGTTTACACCCACCCTTACCTCTTTTAGTGCCACGTACACCTAGCCAAACAAGCACCCTTGGAGGGTACACTATCCCAAAAGGCTCAGCTATCTACTTCAACATTTGGGCCATACAGAGAGATCCTAAAGTTTGGGACAAGCCCTTAGACTTTATGCCCGAAAGATTCTTGAATGAATCTACTGAAAAAGTGTATGATTTCACTGGCAATAACATAGAGTTCTGTCCATTTGGGTCAGGTAAAAAGTTATGTGCAGGGATTCCTCTAGCTGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGCTTTTGAGTGGAAATTGTCTGAAGGTTCAAAGCTTGATTTAGAAGAGAAGTTTGGAATTGTCACCAAGAAGTTGAACCCGTTGGTTGTTATTCCTATGCCAAGGCTTTCCAACTTGGAGCTTTATAATATTACGTAGATTTGGTCTAAATAATTGCCAATATTGGCATCATTTCACTACTCAAATCAAGGATTCCTGTGTTTGTAATATAATGAGGCAACTAAGATGACATATATTCTATGAATGGATTGGCATAAAATAATTTTAGGAGTTTGAACAACCTAATTTGGGGAATCAAAATTTACCTTAAACTTT

Coding sequence (CDS)

ATGTCAATCTCAATTTCCTTCCTCTTCTCATTTTGGTCCGTAGATTCCAACGAAGACGAACAAATTTCAACCGCCCTTCTTTTTATCTTCGTCTTCGTTGTTGCTCTGTTATGGCTCCGACCCAAATTCCGGCGTCCCTCTTTGCCTCCAGGCCCCCGTGGCCTGCCGCTGGTCGGATACCTTCCCTTTTTATCCCGCAATCTCCACCATACGTTCGCCAATTTGGCCGAAATCTACGGCCCAATTTTCAAGCTCCGCCTTGGAACCAAGCTCTGCGTCGTTCTCACCTCCCCTTCCTCCATCAATGAAGCCCTCCGCCACCAAGAAACCGTCTTCGCCAACCGAGATTCCACCGTCAGTGCTCTTCTCGCCACCTACGGCGGAGTCGATATTGTGTTCAGTCAGGACGACGGCGATTGGAAGAAGCTAAGAAAAATCTTCGCCCGGAAAATGCTTAGCAAATCAAATCTGGATGCGTCCTATCCTTTGCGAAGACAAGAAGTAAGAAAAGTGATTAAAGGCGTGTTCGAATCGGGGGGAAACCCAGTTGATATCGGGAAACTGGGTTTCTTCGCCACTGTGAAATCTGTTATGGCAATGACGTGGGGTGGGTCGGGGGGACTGATCGGAGCGGATGGGACTGATTTGGAAGCTAAGTTCAGGGAAGTGGTGGATGAACTGATGGTGTTGGTTGGATCTCCGAATTTGTCGGATCTTTTTCCTGTGTTGGGTCGGTTTGATTTGCAGGGAATTGCGAGGAAGATGAAGAAGGTGATGAATGTTTGTGATGAGATTCTGAATTCCGCCATTGAAGAACAGAGGAAGGTGGGAGAAAATGGTGCGGAAAGGAGAGGATTTTTGCAGTTTCTGTTGGAGGTTAAGGACAGCAAAGATAGTTCAGAGTCCATTACAGATAACCAACTGAAGGCCTTGCTAATGGACATCATCGTTGGAGGAACAGACTCGACATCAACTACAATCGAATGGACAATAACAGAGTTAATACAACAACCAAATACAATGAAGAAAGTCGTGGAAGAATTGACAAAAGTTGTAGGATTAAACCAAATGGTTGAAGAATTTCACTTATCTAAACTACATTATTTAGATGCGGTGATTAAAGAGACACTTCGTTTACACCCACCCTTACCTCTTTTAGTGCCACGTACACCTAGCCAAACAAGCACCCTTGGAGGGTACACTATCCCAAAAGGCTCAGCTATCTACTTCAACATTTGGGCCATACAGAGAGATCCTAAAGTTTGGGACAAGCCCTTAGACTTTATGCCCGAAAGATTCTTGAATGAATCTACTGAAAAAGTGTATGATTTCACTGGCAATAACATAGAGTTCTGTCCATTTGGGTCAGGTAAAAAGTTATGTGCAGGGATTCCTCTAGCTGAGAGGTTGTTGGTTTTAATATTAGCTTCATTGTTGCATGCTTTTGAGTGGAAATTGTCTGAAGGTTCAAAGCTTGATTTAGAAGAGAAGTTTGGAATTGTCACCAAGAAGTTGAACCCGTTGGTTGTTATTCCTATGCCAAGGCTTTCCAACTTGGAGCTTTATAATATTACGTAG

Protein sequence

MSISISFLFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSESITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNIT
Homology
BLAST of Lsi09G003830 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 7.0e-90
Identity = 186/506 (36.76%), Postives = 293/506 (57.91%), Query Frame = 0

Query: 21  QISTALLFIFVFVVALLW-LRPKFRRP---SLPPGPRGLPLVGYLPFLSRNLHHTFANLA 80
           +I + +L+  +F   L + LR  FR+     LPPGP+  P++G L  L    H + A +A
Sbjct: 2   EILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAMA 61

Query: 81  EIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQD 140
           + YGP+  L++G    VV  S S   + L+  +  F++R     A    Y   D+VF+  
Sbjct: 62  QTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAPY 121

Query: 141 DGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGKLGFFATVKS 200
              W+ LRKI +  + S   LD    +R+ EV+ + + +  +G  PV +G+L    T  +
Sbjct: 122 GPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTTNA 181

Query: 201 VMAMTWGGSGGLIGADGTDLE-AKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKM 260
           +  +  G      G+   D + A+F+ +V E+MV+ G  N+ D  P L   D+QG+A KM
Sbjct: 182 LARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAAKM 241

Query: 261 KKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVK--DSKDSSESITDNQLKALLM 320
           KK+    D  L   +EE +  G+   E +  L  L+ +K  D+ +    +TD ++KALL+
Sbjct: 242 KKLHARFDAFLTDILEEHK--GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEIKALLL 301

Query: 321 DIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVI 380
           ++ V GTD++S+T+EW I ELI+ P  + +  +E+ KVVG +++V E  L++L YL+A++
Sbjct: 302 NLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEAIV 361

Query: 381 KETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERF 440
           KET RLHP  PL +PR  S++  + GY IPKGS +  N+WAI RDP  W  PL+F PERF
Sbjct: 362 KETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPERF 421

Query: 441 LNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEG---S 500
           L    +   D  GN+ E  PFG+G+++CAG+ L  R++ L++A+L+HAF W L  G    
Sbjct: 422 LPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQLPE 481

Query: 501 KLDLEEKFGIVTKKLNPLVVIPMPRL 517
            L++EE +G+  ++ +PLVV P PRL
Sbjct: 482 MLNMEEAYGLTLQRADPLVVHPRPRL 505

BLAST of Lsi09G003830 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.1e-87
Identity = 191/505 (37.82%), Postives = 290/505 (57.43%), Query Frame = 0

Query: 22  ISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLAEIYGP 81
           IST LL    F+V LL+ R   + P LPPGP G P++G LP L    H T   L++ YGP
Sbjct: 9   ISTLLL--SSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGP 68

Query: 82  IFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDDGDWK 141
           +F+LRLG+   VV +S +   + LR  +  F+NR     A    Y   D+VF+     W+
Sbjct: 69  LFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWR 128

Query: 142 KLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGV--FESGGNPVDIGK-LGFFATVKSVM 201
            LRK+ +  + S   LD   P+R+ EV  +++ +      G  V++GK L   AT  + +
Sbjct: 129 MLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCAT--NAL 188

Query: 202 AMTWGGSGGLIGADGTDLEA-KFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKMKK 261
           A    G       D    EA +F+E+V ELM L G  N+ D  P LG  DLQG+  KMK+
Sbjct: 189 ARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKR 248

Query: 262 VMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSE-SITDNQLKALLMDII 321
           +    D  L+  IEE +   ++G      L  L+ +K++    E  + +  +KALL+++ 
Sbjct: 249 LHRRYDAFLDRVIEENQANAKSG----DLLSVLIRLKEADAEGEIKLNNTDIKALLLNLF 308

Query: 322 VGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVIKET 381
             GTD++S+T+EW + ELI+ P+ ++K   EL  V+G +++V E  L  L YL AV+KET
Sbjct: 309 TAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQAVVKET 368

Query: 382 LRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERFLNE 441
            RLHP  PL +PR  S+   + GY IPK + +  N+W+I RD  VW+ PL+F P RFL  
Sbjct: 369 FRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLPG 428

Query: 442 STEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEG---SKLD 501
              +  D  GN+ E  PFG+G+++CAG+ L  R++  + A+++HA++W L +G    KLD
Sbjct: 429 GEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQECQKLD 488

Query: 502 LEEKFGIVTKKLNPLVVIPMPRLSN 519
           +EE +G+  ++  PL+V P+PRLS+
Sbjct: 489 MEEAYGLTLQRAVPLMVQPIPRLSH 505

BLAST of Lsi09G003830 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 3.3e-87
Identity = 180/509 (35.36%), Postives = 287/509 (56.39%), Query Frame = 0

Query: 24  TALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLAEIYGPIF 83
           +A++F+ ++   L     +     LPPGP+G P++G LP L    H      + +YGP+ 
Sbjct: 13  SAIVFLSIYYYNLFSNAKRSNGLKLPPGPKGYPVLGNLPQLGAKPHQALQAFSRVYGPLM 72

Query: 84  KLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDDGDWKKL 143
           +LRLG+   VV +SPS   + L++ ++ F  R     A    +   D+VF+     W+ L
Sbjct: 73  RLRLGSVDLVVASSPSVAAQFLKN-DSNFCARPPNSGAEHMAFNYHDLVFAPYGPRWRLL 132

Query: 144 RKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFE--SGGNPVDIGKLGFFATVKSVMAMT 203
           RK+ A  +L    LD +  +R +E+  + + ++E   GG PV++GK     +  ++    
Sbjct: 133 RKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGEPVNVGKEMHVCSTNALSRAM 192

Query: 204 WG---------GSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIA 263
            G         G GG+   +      +F+++V E+M L G  N+ D  P L  FD+QG+ 
Sbjct: 193 MGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFVPWLKPFDIQGVV 252

Query: 264 RKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKD-SSESITDNQLKAL 323
           RKMK+V    +  L+  I E R   + GA     L  L+  +   D S   ITD  +KAL
Sbjct: 253 RKMKRVHRRYNVFLDKFIAECRSSAKPGA--NDLLSVLIGQRGKSDGSGGEITDTAIKAL 312

Query: 324 LMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDA 383
           +++++  GTD++S+TIEW +TELI+ P+ +KK  +E+   VG +++V E  + KL YL A
Sbjct: 313 VLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGRDRLVTESDVPKLPYLQA 372

Query: 384 VIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPE 443
           ++KE  R+HP  PL +PR   +   +GGY IPK S ++ NIWA+ RDP +W  P++F P 
Sbjct: 373 IVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWAMGRDPSIWPDPMEFRPS 432

Query: 444 RFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEG-- 503
           RFL     +  +  GN+ E  PFG+G+++CAG  +  R++   +A+L+HAF+WKL EG  
Sbjct: 433 RFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHSTVATLIHAFDWKLPEGLT 492

Query: 504 -SKLDLEEKFGIVTKKLNPLVVIPMPRLS 518
             K+D+EE FGI  +K  PL+  P+PRL+
Sbjct: 493 AEKIDMEEAFGISLQKAIPLMAHPIPRLA 518

BLAST of Lsi09G003830 vs. ExPASy Swiss-Prot
Match: A0A2K9RG08 (Labd-13Z-ene-9,15,16-triol synthase, chloroplastic OS=Vitex agnus-castus OX=54477 GN=CYP76BK1 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 7.3e-87
Identity = 186/505 (36.83%), Postives = 283/505 (56.04%), Query Frame = 0

Query: 22  ISTALLFIFVFVVA-LLWLRPKFRR---PSLPPGPRGLPLVGYLPFLSRNLHHTFANLAE 81
           IS  ++ + +  +A  L +RP  R+     LPPGP  LP+VG +  L    + +   LA+
Sbjct: 3   ISAVVIVVSLISLAYYLLMRPDLRQGKSAKLPPGPYPLPVVGNIFQLGDKPNRSVTKLAK 62

Query: 82  IYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDD 141
            YGP+  LRLG+ L VV++SP    E L+  + VF++R    S     +  V + F    
Sbjct: 63  KYGPVMSLRLGSILNVVISSPEMAKEVLQKYDHVFSSRFVASSWTAHDHHNVSVPFLPVG 122

Query: 142 GDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESG--GNPVDIGKLGFFATVK 201
             W+K+RK+F   MLS   LD    +R+  ++ + + + ES   G  V+IG     AT+ 
Sbjct: 123 NRWRKMRKLFRDVMLSHPRLDGGRAIRQTMIKNLYEYLLESSKTGKVVNIGAA---ATIT 182

Query: 202 SVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKM 261
           SV   +     G +     D   +F+E  + L +  G PNLSD FP++  FD+QG  R+M
Sbjct: 183 SVNLTSVSLFSGQVFHFDEDESTEFKETFEGLTIAAGRPNLSDFFPIIKPFDIQGCKRQM 242

Query: 262 K----KVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSESITDNQLKAL 321
                K++ + D+I+N  ++E R      A +   L  LL+    K++  S+  N +K +
Sbjct: 243 AFYLGKLLEMFDKIVNERLKEMR-TSPASARKNDMLDTLLDFCRDKENDMSL--NDIKHV 302

Query: 322 LMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDA 381
           L+D+ VG +D+ S TIEW ITEL+  P+ + K  EEL  V+G N++V+E  +  L Y++A
Sbjct: 303 LVDLFVGASDTASRTIEWAITELLLHPDILAKAKEELKNVIGENKLVQESDIPNLPYMEA 362

Query: 382 VIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPE 441
           VIKE  R+HP  P L+PR   + + L GY IPKG+ I+ N+W+I RD KVW  P  F PE
Sbjct: 363 VIKEVFRVHPVGPFLIPRRLEEDTELSGYFIPKGTQIFVNVWSIHRDEKVWPNPESFEPE 422

Query: 442 RFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEG-- 501
           RFLN +     D+ G +    PFGSG+++C G+PLA R+L  ++ + +H+F+WK   G  
Sbjct: 423 RFLNNN----IDYKGQDFVLLPFGSGRRMCPGVPLAHRMLYTMIGTFIHSFDWKFEPGVT 482

Query: 502 -SKLDLEEKFGIVTKKLNPLVVIPM 514
             K+D+ E FGIV  K  PL  IP+
Sbjct: 483 PEKVDMNEAFGIVLHKAVPLKAIPI 497

BLAST of Lsi09G003830 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.2e-86
Identity = 186/506 (36.76%), Postives = 279/506 (55.14%), Query Frame = 0

Query: 25  ALLFIFVFVVALLWL--------RPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLA 84
           A LF+ + +  +L+L        R +     LPPGP   P++G LP +    H T + + 
Sbjct: 2   ATLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMV 61

Query: 85  EIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQD 144
             YGPI  LRLG    VV  S S   + L+  +  FA+R     A    Y   D+VF+  
Sbjct: 62  TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPY 121

Query: 145 DGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGKLGFFATVKS 204
              W+ LRKI +  + S   L+    +R++EV  + + +   G  PV++G+L     V +
Sbjct: 122 GHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNA 181

Query: 205 VMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKMK 264
           +     G    L GAD      +FR +V E+M L G  N+ D  P L   DLQG+A KMK
Sbjct: 182 LGREMIGRR--LFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMK 241

Query: 265 KVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVK--DSKDSSESITDNQLKALLMD 324
           ++    D  L+S ++E    G++  +    L  L+ +K  D      S+TD ++KALL++
Sbjct: 242 RLHKRFDAFLSSILKEHEMNGQD-QKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLN 301

Query: 325 IIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVIK 384
           +   GTD++++T++W I ELI+ P+ M K  EEL  VVG ++ V E  +++L YL AVIK
Sbjct: 302 MFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIK 361

Query: 385 ETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERFL 444
           E  RLHPP PL +P   S++  + GY IPKGS +  NIWAI RDP  W  PL F PERFL
Sbjct: 362 ENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFL 421

Query: 445 NESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEG---SK 504
               +   D  G++ E  PFG+G+++CAG+ L  R +  + A+L+  F+W+L+ G    K
Sbjct: 422 PGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEK 481

Query: 505 LDLEEKFGIVTKKLNPLVVIPMPRLS 518
           L++EE +G+  ++  PLVV P PRL+
Sbjct: 482 LNMEESYGLTLQRAVPLVVHPKPRLA 504

BLAST of Lsi09G003830 vs. ExPASy TrEMBL
Match: A0A1S3ATA1 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 6.1e-246
Identity = 420/517 (81.24%), Postives = 464/517 (89.75%), Query Frame = 0

Query: 8   LFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRN 67
           L SFWSVDSN+  QIST +LFIF+F+ ALLWLR KFRRPSLPPGPRGLPLVGYLPFLS N
Sbjct: 3   LISFWSVDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 68  LHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYG 127
           LHH FA+LA++YG I KL LGTKLC+VLTSPSS+NEALRHQET+F+ RD+TV ALL++YG
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 128 GVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGK 187
           G DIVF+QD+ +WKKLRKIF RKMLSKSNLDASY LRRQEVRKVIKGVFES G P+DIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 188 LGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFD 247
           LGF A +KSVMAMTWGGSGGLIG DGTDL+AKFRE VDE+MVL+ +PNLSDLFPVLGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 248 LQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSESITDNQ 307
           LQG+ RKMKKVM V DEI NSAIEEQRK G NG E RGFLQFLLE+ + +DSSESIT+N 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 308 LKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLH 367
           LKALLMDI++GGTD+TSTT+EW I+ELI+QPNTM KV+EELTKVVGLNQMVEEFHL KL+
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 368 YLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLD 427
           +LDAV+KETLRLHPPLPLLVPRT SQT+T G YTIPKGS IYFNIWAIQRDPKVWD PL+
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 428 FMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLS 487
           F PERFLNES+E +YDFTGN IEFCPFGSGKK CAGIPLA+RLLVLILASLLH FEWKL 
Sbjct: 423 FEPERFLNESSENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLP 482

Query: 488 EGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNI 525
           EGSK D+EEKFGIVTKKLNPLV IP PRL NLELYNI
Sbjct: 483 EGSKFDMEEKFGIVTKKLNPLVTIPTPRLPNLELYNI 519

BLAST of Lsi09G003830 vs. ExPASy TrEMBL
Match: A0A5A7TDF0 (7-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00010 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 4.0e-245
Identity = 419/517 (81.04%), Postives = 463/517 (89.56%), Query Frame = 0

Query: 8   LFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRN 67
           L SFWSV SN+  QIST +LFIF+F+ ALLWLR KFRRPSLPPGPRGLPLVGYLPFLS N
Sbjct: 3   LISFWSVHSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 68  LHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYG 127
           LHH FA+LA++YG I KL LGTKLC+VLTSPSS+NEALRHQET+F+ RD+TV ALL++YG
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 128 GVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGK 187
           G DIVF+QD+ +WKKLRKIF RKMLSKSNLDASY LRRQEVRKVIKGVFES G P+DIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 188 LGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFD 247
           LGF A +KSVMAMTWGGSGGLIG DGTDL+AKFRE VDE+MVL+ +PNLSDLFPVLGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 248 LQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSESITDNQ 307
           LQG+ RKMKKVM V DEI NSAIEEQRK G NG E RGFLQFLLE+ + +DSSESIT+N 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 308 LKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLH 367
           LKALLMDI++GGTD+TSTT+EW I+ELI+QPNTM KV+EELTKVVGLNQMVEEFHL KL+
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 368 YLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLD 427
           +LDAV+KETLRLHPPLPLLVPRT SQT+T G YTIPKGS IYFNIWAIQRDPKVWD PL+
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 428 FMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLS 487
           F PERFLNES+E +YDFTGN IEFCPFGSGKK CAGIPLA+RLLVLILASLLH FEWKL 
Sbjct: 423 FEPERFLNESSENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLP 482

Query: 488 EGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNI 525
           EGSK D+EEKFGIVTKKLNPLV IP PRL NLELYNI
Sbjct: 483 EGSKFDMEEKFGIVTKKLNPLVAIPTPRLPNLELYNI 519

BLAST of Lsi09G003830 vs. ExPASy TrEMBL
Match: A0A5A7T9J1 (Flavonoid 3'-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00030 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 9.8e-244
Identity = 423/522 (81.03%), Postives = 460/522 (88.12%), Query Frame = 0

Query: 2   SISISFLFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYL 61
           S+S  FLF     DSN+  QIST LLFIF+F+ ALLWLR KFR+  LPPGPRGLPLVGYL
Sbjct: 3   SVSTPFLF-----DSNKHSQISTPLLFIFLFIFALLWLRSKFRQSPLPPGPRGLPLVGYL 62

Query: 62  PFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSA 121
           PFLS NLHH FA+LA+IYGPIFKLRLG KLC VLTSPSSINE LRH ETVF NRD+TVSA
Sbjct: 63  PFLSTNLHHNFADLAKIYGPIFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSA 122

Query: 122 LLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGN 181
            L TYGG DIVFSQ++GDWKKLRKIFARKMLSK+NLDASYPLRRQEVRK+IKGV ES G 
Sbjct: 123 RLCTYGGSDIVFSQNEGDWKKLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGT 182

Query: 182 PVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFP 241
           P+D G+L F A VKSVMAM WGGSGGL G  GTDLEAKFREVVDELMVL+G+PNLSDLFP
Sbjct: 183 PIDFGELSFLAAVKSVMAMIWGGSGGLTGVAGTDLEAKFREVVDELMVLLGTPNLSDLFP 242

Query: 242 VLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSE 301
           VLG  DLQGIARKMKKV N CDEILNSAIE QRK G NG E RGFL+FLL+V + +D+SE
Sbjct: 243 VLGCLDLQGIARKMKKVSNRCDEILNSAIEGQRKKGRNGLENRGFLEFLLKVMEGEDNSE 302

Query: 302 SITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEF 361
           SIT+NQLK LL+DI++GGTD+TST IEWT+TEL+QQPNTMKKV+EELT VVGLNQMVEEF
Sbjct: 303 SITENQLKGLLIDIVIGGTDTTSTVIEWTMTELLQQPNTMKKVIEELTNVVGLNQMVEEF 362

Query: 362 HLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKV 421
           HLSKLHYLDA IKETLRLHPP+ LLVPR  S+T+TL GYTIPKGS IYFN+WAIQRDPKV
Sbjct: 363 HLSKLHYLDAAIKETLRLHPPVSLLVPRVSSKTTTLEGYTIPKGSTIYFNVWAIQRDPKV 422

Query: 422 WDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHA 481
           W+ PLDFMPERFLNES EK+YDF+GN IEFCPFGSGKK CAGIPLAERLLVLILASLLH 
Sbjct: 423 WENPLDFMPERFLNESNEKMYDFSGNKIEFCPFGSGKKSCAGIPLAERLLVLILASLLHG 482

Query: 482 FEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYN 524
           FEWKL EGSKLDLEEKFGIVT+KLNPLVVIP PRLSNLELYN
Sbjct: 483 FEWKLPEGSKLDLEEKFGIVTRKLNPLVVIPTPRLSNLELYN 519

BLAST of Lsi09G003830 vs. ExPASy TrEMBL
Match: A0A1S3ATW0 (flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482653 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.8e-243
Identity = 423/522 (81.03%), Postives = 459/522 (87.93%), Query Frame = 0

Query: 2   SISISFLFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYL 61
           S+S  FLF     DSN+  QIST LLFIF+F+ ALLWLR KFR+  LPPGPRGLPLVGYL
Sbjct: 3   SVSTPFLF-----DSNKHSQISTPLLFIFLFISALLWLRSKFRQSPLPPGPRGLPLVGYL 62

Query: 62  PFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSA 121
           PFLS NLHH FA+LA+IYGPIFKLRLG KLC VLTSPSSINE LRH ETVF NRD+TVSA
Sbjct: 63  PFLSTNLHHNFADLAKIYGPIFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSA 122

Query: 122 LLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGN 181
            L TYGG DIVFSQ++GDWKKLRKIFARKMLSK+NLDASYPLRRQEVRK+IKGV ES G 
Sbjct: 123 RLCTYGGSDIVFSQNEGDWKKLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGT 182

Query: 182 PVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFP 241
           P+D G+L F A VKSVMAM WGGSGGL G   TDLEAKFREVVDELMVL+G+PNLSDLFP
Sbjct: 183 PIDFGELSFLAAVKSVMAMIWGGSGGLTGVAVTDLEAKFREVVDELMVLLGTPNLSDLFP 242

Query: 242 VLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSE 301
           VLG  DLQGIARKMKKV N CDEILNSAIE QRK G NG E RGFL+FLLEV + +D+SE
Sbjct: 243 VLGCLDLQGIARKMKKVSNRCDEILNSAIEGQRKKGRNGLENRGFLEFLLEVMEGEDNSE 302

Query: 302 SITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEF 361
           SIT+NQLK LL+DI++GGTD+TST IEWT+TEL+QQPNTMKKV+EELT VVGLNQMVEEF
Sbjct: 303 SITENQLKGLLIDIVIGGTDTTSTVIEWTMTELLQQPNTMKKVIEELTNVVGLNQMVEEF 362

Query: 362 HLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKV 421
           HLSKLHYLDA IKETLRLHPP+ LLVPR  S+T+TL GYTIPKGS IYFN+WAIQRDPKV
Sbjct: 363 HLSKLHYLDAAIKETLRLHPPVSLLVPRVSSKTTTLEGYTIPKGSTIYFNVWAIQRDPKV 422

Query: 422 WDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHA 481
           W+ PLDFMPERFLNES EK+YDF+GN IEFCPFGSGKK CAGIPLAERLLVLILASLLH 
Sbjct: 423 WENPLDFMPERFLNESNEKMYDFSGNKIEFCPFGSGKKSCAGIPLAERLLVLILASLLHG 482

Query: 482 FEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYN 524
           FEWKL EGSKLDLEEKFGIVT+KLNPLVVIP PRLSNLELYN
Sbjct: 483 FEWKLPEGSKLDLEEKFGIVTRKLNPLVVIPTPRLSNLELYN 519

BLAST of Lsi09G003830 vs. ExPASy TrEMBL
Match: A0A6J1DW75 (7-ethoxycoumarin O-deethylase-like OS=Momordica charantia OX=3673 GN=LOC111023676 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 9.5e-207
Identity = 375/528 (71.02%), Postives = 437/528 (82.77%), Query Frame = 0

Query: 1   MSISISFLFSFWSVD-SNEDEQISTALLFIFVFVVALLWLR----PKFRRPSLPPGPRGL 60
           +S+   F FSFW+VD SNED+QIS A+L IFV + AL W R    PK R P  PP PRGL
Sbjct: 23  VSVPFVFFFSFWTVDSSNEDKQISIAVLSIFVLIFALFWFRRSGEPKLRLPPSPPSPRGL 82

Query: 61  PLVGYLPFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANR 120
           PL+GYLPFLS NLH TFA+LA IY P+FKLRLGTKLCVVLTSPS++NEAL  Q+TVFANR
Sbjct: 83  PLLGYLPFLSLNLHRTFADLAGIYSPVFKLRLGTKLCVVLTSPSAVNEALHRQDTVFANR 142

Query: 121 DSTVSALLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGV 180
           D TV ALLA+YGG DIV + D  DWKKLRKIFARKMLSKSNL+ASY +RR+EVR+ IKGV
Sbjct: 143 DPTVCALLASYGGSDIVITSDGADWKKLRKIFARKMLSKSNLEASYCVRRREVREAIKGV 202

Query: 181 FESGGNPVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPN 240
           FES G P+D+G++GF AT+KSVMAM +GG+G  IG    DLE   RE + ELMVL+G+PN
Sbjct: 203 FESAGTPIDVGEVGFLATLKSVMAMIFGGAGRRIGG---DLEGDLREKMGELMVLLGTPN 262

Query: 241 LSDLFPVLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKD 300
           +SD+FP LG FDLQGI R+ K+VM + DEIL+ AIEEQ KVGENG    GFLQ+LLE+ D
Sbjct: 263 VSDIFPALGGFDLQGIGRRTKEVMLMIDEILDLAIEEQ-KVGENGG---GFLQWLLELND 322

Query: 301 SKDSSESITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLN 360
           ++D S+SIT NQLKALL+DI+VGGTD+TSTTIEW + EL+Q  N M+KV EELT+VVGLN
Sbjct: 323 TQDCSDSITTNQLKALLVDIVVGGTDTTSTTIEWAMAELMQHRNIMEKVTEELTQVVGLN 382

Query: 361 QMVEEFHLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAI 420
           QMVEEFHLSKL +L+AVIKETLRLHPPLPLLVPR  +QTSTLGGY+IPKGSAIYFNIWAI
Sbjct: 383 QMVEEFHLSKLCFLNAVIKETLRLHPPLPLLVPRIAAQTSTLGGYSIPKGSAIYFNIWAI 442

Query: 421 QRDPKVWDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLIL 480
           QRDPKVWD PL FMP RFLNE   + YDFTGN +EFCPFGSG++ C GIPLAER+LV IL
Sbjct: 443 QRDPKVWDNPLKFMPRRFLNE--PEGYDFTGNCMEFCPFGSGRRSCVGIPLAERMLVFIL 502

Query: 481 ASLLHAFEWKL-SEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELY 523
           ASLLH+FEW+L  + S LDLE+KFGIVTKKL PLV +P+PRLSNLEL+
Sbjct: 503 ASLLHSFEWELPKDSSVLDLEDKFGIVTKKLKPLVAVPVPRLSNLELF 541

BLAST of Lsi09G003830 vs. NCBI nr
Match: XP_038906861.1 (labd-13Z-ene-9,15,16-triol synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 898.7 bits (2321), Expect = 2.5e-257
Identity = 448/525 (85.33%), Postives = 482/525 (91.81%), Query Frame = 0

Query: 1   MSISISFLFSFWSVD-SNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVG 60
           M++S  F+FSFWSVD SN D+Q STA+L IFVFV ALLWLRPKFRRPSLPPGPRGLPLVG
Sbjct: 1   MAVSAPFVFSFWSVDYSNGDKQTSTAVLLIFVFVFALLWLRPKFRRPSLPPGPRGLPLVG 60

Query: 61  YLPFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTV 120
           YLPFLS NLHHTFA+LA+IYGPIFKLRLGTKLCVVL+SPSS+NEAL HQETV  NRDST+
Sbjct: 61  YLPFLSGNLHHTFADLAQIYGPIFKLRLGTKLCVVLSSPSSVNEALHHQETVLDNRDSTI 120

Query: 121 SALLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESG 180
           SALLATYGG DI+FSQD+GDWKKLRKIF RKMLSKSNLDASY LRR+EVRKVIKG FES 
Sbjct: 121 SALLATYGGADILFSQDEGDWKKLRKIFTRKMLSKSNLDASYSLRRKEVRKVIKGAFESA 180

Query: 181 GNPVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDL 240
           G+P++IGKLGFFATVKSVMAMTWGGSGGLIG D  DLEAKFREVVDELM+LVG+PNLSDL
Sbjct: 181 GSPINIGKLGFFATVKSVMAMTWGGSGGLIGMDEADLEAKFREVVDELMILVGTPNLSDL 240

Query: 241 FPVLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDS 300
           FPVLGRFDLQGIARKMKKVMNV  EILNSAIEEQRK+G +G ERRGFLQ LLE KDS+DS
Sbjct: 241 FPVLGRFDLQGIARKMKKVMNVFYEILNSAIEEQRKMGGHGVERRGFLQCLLEFKDSEDS 300

Query: 301 SESITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVE 360
           SESITDNQLK LLMDII+GGTD+T+TTIEW I EL+QQPNTMKKVVEELTKVVGLNQMVE
Sbjct: 301 SESITDNQLKGLLMDIIIGGTDTTATTIEWAIAELMQQPNTMKKVVEELTKVVGLNQMVE 360

Query: 361 EFHLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDP 420
           +FHLSKLHYLDAV+KETLRLHPPL LLVPRT +QT  LGGYTIPK SAIYFNIWAIQRDP
Sbjct: 361 DFHLSKLHYLDAVVKETLRLHPPLSLLVPRTATQTIILGGYTIPKNSAIYFNIWAIQRDP 420

Query: 421 KVWDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLL 480
           KVWD PL+F PERFLNE T   YDFTGNNI+FCPFGSGKK+CAGIPLAERLLVLILASLL
Sbjct: 421 KVWDNPLNFWPERFLNEFTRNAYDFTGNNIKFCPFGSGKKVCAGIPLAERLLVLILASLL 480

Query: 481 HAFEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNI 525
           HAFEW+L EGSKLD EE FGIVT+KLNPLV IP+PRLSNLELYNI
Sbjct: 481 HAFEWELPEGSKLDSEETFGIVTRKLNPLVAIPIPRLSNLELYNI 525

BLAST of Lsi09G003830 vs. NCBI nr
Match: XP_004147610.2 (flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KAE8647886.1 hypothetical protein Csa_000301 [Cucumis sativus])

HSP 1 Score: 862.4 bits (2227), Expect = 1.9e-246
Identity = 428/523 (81.84%), Postives = 464/523 (88.72%), Query Frame = 0

Query: 2   SISISFLFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYL 61
           ++SI FLFSFWS+D N+  QISTALLF F+F+ ALLWL+ KF+ P LPPGPRGLPL+GYL
Sbjct: 3   TLSIPFLFSFWSLDFNKHSQISTALLFFFLFIFALLWLQSKFQHPPLPPGPRGLPLLGYL 62

Query: 62  PFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSA 121
           PFLS NLHHTF NLA++YGPIFKLRLGTKLCVVLTSPSS+NE LRHQETVF NRD+TVSA
Sbjct: 63  PFLSTNLHHTFNNLAKMYGPIFKLRLGTKLCVVLTSPSSVNEVLRHQETVFDNRDATVSA 122

Query: 122 LLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGN 181
           LLATYGGVDIVFSQ++  WKKLRKIFARKMLSKSNLDASYPLRR+EVRK+IKGV ES G 
Sbjct: 123 LLATYGGVDIVFSQNESIWKKLRKIFARKMLSKSNLDASYPLRRREVRKIIKGVLESAGT 182

Query: 182 PVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFP 241
           P+DI KL   A VKSVMAMTWGGS GLIG DG DLEAKF EVV+ELMVL+G+PNLSDLFP
Sbjct: 183 PIDISKLSLMAAVKSVMAMTWGGSRGLIGVDGADLEAKFMEVVNELMVLLGTPNLSDLFP 242

Query: 242 VLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSE 301
           VLG  DLQGI RKMKKVMNV D I NSAIEEQRK G +G E RGFLQ LLEV + +DSSE
Sbjct: 243 VLGGLDLQGIGRKMKKVMNVFDGIFNSAIEEQRKRGGDGMENRGFLQLLLEVMEGEDSSE 302

Query: 302 SITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEF 361
           SITD +LKALL+DII+GGTD+TSTTIEWTI ELIQQPN MKKV EELTKVVGLNQMVEEF
Sbjct: 303 SITDKELKALLVDIIIGGTDTTSTTIEWTIAELIQQPNIMKKVKEELTKVVGLNQMVEEF 362

Query: 362 HLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKV 421
           HLSKLHYLDA IKETLRLHPP+PLLVPRT +Q +TL GYTIPK S IYFNIWAIQRDPK+
Sbjct: 363 HLSKLHYLDAAIKETLRLHPPVPLLVPRTTNQRTTLEGYTIPKSSTIYFNIWAIQRDPKI 422

Query: 422 WDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHA 481
           WD PLDFMPERFLNES E +YDFTGN IEFCPFG+GKKLC GIPLAERLLVLILASLLH 
Sbjct: 423 WDNPLDFMPERFLNESNENMYDFTGNKIEFCPFGAGKKLCVGIPLAERLLVLILASLLHG 482

Query: 482 FEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNI 525
           FEWKL EGS LDLEEKFGIV+KKLNPLVVIP PRLSNLELYN+
Sbjct: 483 FEWKLPEGSTLDLEEKFGIVSKKLNPLVVIPTPRLSNLELYNM 525

BLAST of Lsi09G003830 vs. NCBI nr
Match: XP_008437141.1 (PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo])

HSP 1 Score: 859.8 bits (2220), Expect = 1.3e-245
Identity = 420/517 (81.24%), Postives = 464/517 (89.75%), Query Frame = 0

Query: 8   LFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRN 67
           L SFWSVDSN+  QIST +LFIF+F+ ALLWLR KFRRPSLPPGPRGLPLVGYLPFLS N
Sbjct: 3   LISFWSVDSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 68  LHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYG 127
           LHH FA+LA++YG I KL LGTKLC+VLTSPSS+NEALRHQET+F+ RD+TV ALL++YG
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 128 GVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGK 187
           G DIVF+QD+ +WKKLRKIF RKMLSKSNLDASY LRRQEVRKVIKGVFES G P+DIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 188 LGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFD 247
           LGF A +KSVMAMTWGGSGGLIG DGTDL+AKFRE VDE+MVL+ +PNLSDLFPVLGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 248 LQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSESITDNQ 307
           LQG+ RKMKKVM V DEI NSAIEEQRK G NG E RGFLQFLLE+ + +DSSESIT+N 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 308 LKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLH 367
           LKALLMDI++GGTD+TSTT+EW I+ELI+QPNTM KV+EELTKVVGLNQMVEEFHL KL+
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 368 YLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLD 427
           +LDAV+KETLRLHPPLPLLVPRT SQT+T G YTIPKGS IYFNIWAIQRDPKVWD PL+
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 428 FMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLS 487
           F PERFLNES+E +YDFTGN IEFCPFGSGKK CAGIPLA+RLLVLILASLLH FEWKL 
Sbjct: 423 FEPERFLNESSENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLP 482

Query: 488 EGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNI 525
           EGSK D+EEKFGIVTKKLNPLV IP PRL NLELYNI
Sbjct: 483 EGSKFDMEEKFGIVTKKLNPLVTIPTPRLPNLELYNI 519

BLAST of Lsi09G003830 vs. NCBI nr
Match: KAA0039671.1 (7-ethoxycoumarin O-deethylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 857.1 bits (2213), Expect = 8.2e-245
Identity = 419/517 (81.04%), Postives = 463/517 (89.56%), Query Frame = 0

Query: 8   LFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYLPFLSRN 67
           L SFWSV SN+  QIST +LFIF+F+ ALLWLR KFRRPSLPPGPRGLPLVGYLPFLS N
Sbjct: 3   LISFWSVHSNKLNQISTPILFIFLFIFALLWLRSKFRRPSLPPGPRGLPLVGYLPFLSPN 62

Query: 68  LHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYG 127
           LHH FA+LA++YG I KL LGTKLC+VLTSPSS+NEALRHQET+F+ RD+TV ALL++YG
Sbjct: 63  LHHNFADLAKVYGSILKLHLGTKLCIVLTSPSSVNEALRHQETIFSIRDTTVVALLSSYG 122

Query: 128 GVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGNPVDIGK 187
           G DIVF+QD+ +WKKLRKIF RKMLSKSNLDASY LRRQEVRKVIKGVFES G P+DIGK
Sbjct: 123 GSDIVFTQDENNWKKLRKIFTRKMLSKSNLDASYSLRRQEVRKVIKGVFESAGTPIDIGK 182

Query: 188 LGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFD 247
           LGF A +KSVMAMTWGGSGGLIG DGTDL+AKFRE VDE+MVL+ +PNLSDLFPVLGRFD
Sbjct: 183 LGFVAALKSVMAMTWGGSGGLIGVDGTDLDAKFREAVDEMMVLLATPNLSDLFPVLGRFD 242

Query: 248 LQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSESITDNQ 307
           LQG+ RKMKKVM V DEI NSAIEEQRK G NG E RGFLQFLLE+ + +DSSESIT+N 
Sbjct: 243 LQGVVRKMKKVMCVFDEIFNSAIEEQRKRGGNGMENRGFLQFLLEIMEGEDSSESITENN 302

Query: 308 LKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLH 367
           LKALLMDI++GGTD+TSTT+EW I+ELI+QPNTM KV+EELTKVVGLNQMVEEFHL KL+
Sbjct: 303 LKALLMDIVIGGTDTTSTTVEWAISELIKQPNTMNKVIEELTKVVGLNQMVEEFHLPKLN 362

Query: 368 YLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLD 427
           +LDAV+KETLRLHPPLPLLVPRT SQT+T G YTIPKGS IYFNIWAIQRDPKVWD PL+
Sbjct: 363 FLDAVVKETLRLHPPLPLLVPRTSSQTTTFGEYTIPKGSTIYFNIWAIQRDPKVWDNPLN 422

Query: 428 FMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLS 487
           F PERFLNES+E +YDFTGN IEFCPFGSGKK CAGIPLA+RLLVLILASLLH FEWKL 
Sbjct: 423 FEPERFLNESSENLYDFTGNRIEFCPFGSGKKSCAGIPLAKRLLVLILASLLHGFEWKLP 482

Query: 488 EGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYNI 525
           EGSK D+EEKFGIVTKKLNPLV IP PRL NLELYNI
Sbjct: 483 EGSKFDMEEKFGIVTKKLNPLVAIPTPRLPNLELYNI 519

BLAST of Lsi09G003830 vs. NCBI nr
Match: KAA0039673.1 (flavonoid 3'-monooxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 852.4 bits (2201), Expect = 2.0e-243
Identity = 423/522 (81.03%), Postives = 460/522 (88.12%), Query Frame = 0

Query: 2   SISISFLFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRRPSLPPGPRGLPLVGYL 61
           S+S  FLF     DSN+  QIST LLFIF+F+ ALLWLR KFR+  LPPGPRGLPLVGYL
Sbjct: 3   SVSTPFLF-----DSNKHSQISTPLLFIFLFIFALLWLRSKFRQSPLPPGPRGLPLVGYL 62

Query: 62  PFLSRNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSA 121
           PFLS NLHH FA+LA+IYGPIFKLRLG KLC VLTSPSSINE LRH ETVF NRD+TVSA
Sbjct: 63  PFLSTNLHHNFADLAKIYGPIFKLRLGAKLCFVLTSPSSINEVLRHHETVFDNRDATVSA 122

Query: 122 LLATYGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESGGN 181
            L TYGG DIVFSQ++GDWKKLRKIFARKMLSK+NLDASYPLRRQEVRK+IKGV ES G 
Sbjct: 123 RLCTYGGSDIVFSQNEGDWKKLRKIFARKMLSKANLDASYPLRRQEVRKIIKGVLESAGT 182

Query: 182 PVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFP 241
           P+D G+L F A VKSVMAM WGGSGGL G  GTDLEAKFREVVDELMVL+G+PNLSDLFP
Sbjct: 183 PIDFGELSFLAAVKSVMAMIWGGSGGLTGVAGTDLEAKFREVVDELMVLLGTPNLSDLFP 242

Query: 242 VLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKDSSE 301
           VLG  DLQGIARKMKKV N CDEILNSAIE QRK G NG E RGFL+FLL+V + +D+SE
Sbjct: 243 VLGCLDLQGIARKMKKVSNRCDEILNSAIEGQRKKGRNGLENRGFLEFLLKVMEGEDNSE 302

Query: 302 SITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEF 361
           SIT+NQLK LL+DI++GGTD+TST IEWT+TEL+QQPNTMKKV+EELT VVGLNQMVEEF
Sbjct: 303 SITENQLKGLLIDIVIGGTDTTSTVIEWTMTELLQQPNTMKKVIEELTNVVGLNQMVEEF 362

Query: 362 HLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKV 421
           HLSKLHYLDA IKETLRLHPP+ LLVPR  S+T+TL GYTIPKGS IYFN+WAIQRDPKV
Sbjct: 363 HLSKLHYLDAAIKETLRLHPPVSLLVPRVSSKTTTLEGYTIPKGSTIYFNVWAIQRDPKV 422

Query: 422 WDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHA 481
           W+ PLDFMPERFLNES EK+YDF+GN IEFCPFGSGKK CAGIPLAERLLVLILASLLH 
Sbjct: 423 WENPLDFMPERFLNESNEKMYDFSGNKIEFCPFGSGKKSCAGIPLAERLLVLILASLLHG 482

Query: 482 FEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELYN 524
           FEWKL EGSKLDLEEKFGIVT+KLNPLVVIP PRLSNLELYN
Sbjct: 483 FEWKLPEGSKLDLEEKFGIVTRKLNPLVVIPTPRLSNLELYN 519

BLAST of Lsi09G003830 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 467.6 bits (1202), Expect = 1.3e-131
Identity = 237/501 (47.31%), Postives = 345/501 (68.86%), Query Frame = 0

Query: 27  LFIFVFVVALLW-LRPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLAEIYGPIFKL 86
           + I   V ++LW +  +  +PSLPPGPRGLP+VG LPFL  +LH  FANLA+ +GPIFKL
Sbjct: 20  IVILTTVFSILWYIFKRSPQPSLPPGPRGLPIVGNLPFLDPDLHTYFANLAQSHGPIFKL 79

Query: 87  RLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDDGDWKKLRK 146
            LG+KL +V+ SPS   E L+ Q+  F+NRD  ++   ATYGG+DIV++    +W++LRK
Sbjct: 80  NLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPYGAEWRQLRK 139

Query: 147 IFARKMLSKSNLDASYPLRRQEVRKVIKGVFESG--GNPVDIGKLGFFATVKSVMAMTWG 206
           I   K+LS+  LD+ Y LRR+EVR+  + ++E G   +PV +G   F   +   M M WG
Sbjct: 140 ICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMMNLTMNMLWG 199

Query: 207 GSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKMKKVMNVCD 266
           GS  +   +   +  +F+ V+ E+  L+  PN+SD FP L RFDLQG+ ++M       D
Sbjct: 200 GS--VKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKRMGVCARELD 259

Query: 267 EILNSAIEEQRKV-GENGAERRGFLQFLLEVKDSK-DSSESITDNQLKALLMDIIVGGTD 326
            +L+ AIE+ + + G +  E + FLQ+L+++KD + DS   IT N +KALL D++VGGTD
Sbjct: 260 AVLDRAIEQMKPLRGRDDDEVKDFLQYLMKLKDQEGDSEVPITINHVKALLTDMVVGGTD 319

Query: 327 STSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVIKETLRLHP 386
           +++ TIE+ + EL+  P  +K+  EEL +VVG + +VEE H+++L Y+ A++KETLRLHP
Sbjct: 320 TSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYILAIMKETLRLHP 379

Query: 387 PLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERFLNESTEKV 446
            LPLLVP  P++ + +GGYTIPK + I+ N+W+IQRDP VW+ P +F PERFL+ ++   
Sbjct: 380 TLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRPERFLDNNS--- 439

Query: 447 YDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEGSKLDLEEKFGIV 506
            DFTG N  + PFGSG+++CAG+ LAER+++  LA+LLH+F+WK+ EG  LDL+EKFGIV
Sbjct: 440 CDFTGANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIPEGHVLDLKEKFGIV 499

Query: 507 TKKLNPLVVIPMPRLSNLELY 523
            K   PLV +P+PR S+  LY
Sbjct: 500 LKLKIPLVALPIPRFSDSNLY 515

BLAST of Lsi09G003830 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.7e-131
Identity = 236/504 (46.83%), Postives = 350/504 (69.44%), Query Frame = 0

Query: 27  LFIFVFVVALLW-LRPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLAEIYGPIFKL 86
           + I + + ++LW L  +  +P LPPGPRGLP+VG LPFL  +LH  F  LAE YGPIFKL
Sbjct: 20  ILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTKLAESYGPIFKL 79

Query: 87  RLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDDGDWKKLRK 146
            LG+KL VV+ +PS   E L+ Q+  F+N D  ++A   TYGG+D+V+     +W+ LRK
Sbjct: 80  NLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRK 139

Query: 147 IFARKMLSKSNLDASYPLRRQEVRKVIKGVFESG--GNPVDIGKLGFFATVKSVMAMTWG 206
           +   K+LS   L++ Y LRR+E+R+  + +++ G   +PV++G+  F   +   M M WG
Sbjct: 140 VCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMNLTMNMLWG 199

Query: 207 GSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKMKKVMNVCD 266
           GS  +   +   +  +F+EV+ E+  L+G PN+SD FP L RFDLQG+ +KM       D
Sbjct: 200 GS--VKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKMHVCARELD 259

Query: 267 EILNSAIEEQR----KVGENGAERRGFLQFLLEVKDSK-DSSESITDNQLKALLMDIIVG 326
            IL+ AIE+ +    + G++G E + FLQ L+++KD + DS   IT N +KA+L+D++VG
Sbjct: 260 AILDRAIEQMQLLRTRDGDDG-ECKDFLQHLMKLKDQEADSEVPITVNHVKAVLVDLVVG 319

Query: 327 GTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVIKETLR 386
           GTD+++ TIE+ + ELI++P  MK+  +EL +VVG + ++EE H+++L ++ A++KETLR
Sbjct: 320 GTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFISAIMKETLR 379

Query: 387 LHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERFLNEST 446
           L+P +PLLVP  PS+T+ +GGYTIPK + I+ N+W+IQRDP VW+ P +F PERFL+   
Sbjct: 380 LYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFLD--- 439

Query: 447 EKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEGSKLDLEEKF 506
           +K  DFTG +  + PFGSG+++CAGI LAER+++  LA+LLH+F+WK+ EG  LDL+EKF
Sbjct: 440 KKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEGHILDLKEKF 499

Query: 507 GIVTKKLNPLVVIPMPRLSNLELY 523
           GIV K  +PLV +P+PRLSN  LY
Sbjct: 500 GIVLKLKSPLVALPVPRLSNSNLY 517

BLAST of Lsi09G003830 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 461.5 bits (1186), Expect = 9.3e-130
Identity = 235/504 (46.63%), Postives = 346/504 (68.65%), Query Frame = 0

Query: 27  LFIFVFVVALLW-LRPKFRRPSLPPGPRGLPLVGYLPFLSRNLHHTFANLAEIYGPIFKL 86
           + I     ++LW +  +  +P LPPGPRGLP+VG LPFL  +LH  F  LA+ +GPIFKL
Sbjct: 22  VLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKL 81

Query: 87  RLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQDDGDWKKLRK 146
            LG+KL VV+ SPS  +E L+ Q+  F+N D  ++A   TYGG+D+V+     +W+ LRK
Sbjct: 82  NLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRK 141

Query: 147 IFARKMLSKSNLDASYPLRRQEVRKVIKGVFESG--GNPVDIGKLGFFATVKSVMAMTWG 206
           + A K+ S+  LD+ Y LRR+E+R+  + +++ G   +PV++G+  F   +  +M M WG
Sbjct: 142 VCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWG 201

Query: 207 GSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARKMKKVMNVCD 266
           GS  +   D   +  +F+ V+ E+  L+G PN+SD FP+L RFDLQG+ +KM       D
Sbjct: 202 GS--VKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLD 261

Query: 267 EILNSAIEEQRKV----GENGAERRGFLQFLLEVKDSK-DSSESITDNQLKALLMDIIVG 326
            IL+ AIE+ +++    G++G E + FLQ L++++D + DS   IT N +KA+LMD++VG
Sbjct: 262 AILDRAIEQMQRLRSRDGDDG-ECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMVVG 321

Query: 327 GTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAVIKETLR 386
           GT+S++ TIE+ + ELI  P  M++  +EL +VVG + +VEE H++ L Y+ AV+KETLR
Sbjct: 322 GTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKETLR 381

Query: 387 LHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPERFLNEST 446
           L+P +PLLVP  PS+T+ +GGYTIPK + I+ N+W+IQRDP VW+ P +F PERFL+   
Sbjct: 382 LYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFLD--- 441

Query: 447 EKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEGSKLDLEEKF 506
           +K  DFTG +  + PFGSG+++CAGI LAER+++  LA+LLH+F+W + +G  LDLEEKF
Sbjct: 442 KKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHVLDLEEKF 501

Query: 507 GIVTKKLNPLVVIPMPRLSNLELY 523
           GIV K   PLV +P+PRLSN   Y
Sbjct: 502 GIVLKLKTPLVALPIPRLSNSNFY 519

BLAST of Lsi09G003830 vs. TAIR 10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 232/524 (44.27%), Postives = 349/524 (66.60%), Query Frame = 0

Query: 8   LFSFWSVDSNEDEQISTALLFIFVFVVALLWLRPKFRR--PSLPPGPRGLPLVGYLPFLS 67
           +F+F+++ +  D         +   V   +W+  K +R  P LPPGPRGLP+VG LPFL 
Sbjct: 4   IFTFFTIQNRNDHFDFVLATIVISSVFWYIWVYVKSKRLFPPLPPGPRGLPIVGNLPFLH 63

Query: 68  RNLHHTFANLAEIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLAT 127
             LH  F +LA+ +GP+FKL LG KL +V+TS  +  + LR  + +FAN D  V+  L+T
Sbjct: 64  PELHTYFHSLAQKHGPVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLST 123

Query: 128 YGGVDIVFSQDDGDWKKLRKIFARKMLSKSNLDAS--YPLRRQEVRKVIKGVFE--SGGN 187
           YGGVDIV+S    +W  LRKI   KMLS + LD++    LRRQE R+ ++ + +    G 
Sbjct: 124 YGGVDIVWSPYGPEWPMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGL 183

Query: 188 PVDIGKLGFFATVKSVMAMTWGGSGGLIGADGTDLE---AKFREVVDELMVLVGSPNLSD 247
            V++G+  F   +  V  M WG +     AD  + E   A+F E++ E++ +VG PN+SD
Sbjct: 184 AVNVGEQIFVTILNVVTQMLWGET----VADDEEREKVGAEFLELITEIIDVVGKPNVSD 243

Query: 248 LFPVLGRFDLQGIARKMKKVMNVCDEILNSAIEEQRKVGENGAERRGFLQFLLEVKDSKD 307
            FPVL RFDLQG+A+++++     D + +  I  QR   + G++  G   FL+ + ++KD
Sbjct: 244 FFPVLSRFDLQGLAKRVRRSAQRMDRMFDRII-SQRMGMDKGSKGNGG-DFLMVLLNAKD 303

Query: 308 SSESITDNQLKALLMDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMV 367
             E+++ N +KALLMD+++GGTD++  TIE+ + ELI +   MK+  +EL KVVG N +V
Sbjct: 304 EDENMSMNHVKALLMDMVLGGTDTSLNTIEFAMAELINKLEIMKRAQQELDKVVGKNNIV 363

Query: 368 EEFHLSKLHYLDAVIKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRD 427
           EE H++KL Y+ +++KETLRLHP LPLL+PR PS+T+ +GGYTIP  S ++ N+WAI R+
Sbjct: 364 EEKHITKLPYILSIMKETLRLHPALPLLIPRCPSETTVIGGYTIPNDSKVFINVWAIHRN 423

Query: 428 PKVWDKPLDFMPERFLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASL 487
           P VW+ PL+F P+RFL    +K YDF+GN+  + PFGSG+++CAG+ +AE++++  LA+L
Sbjct: 424 PNVWENPLEFNPDRFL----DKGYDFSGNDYSYFPFGSGRRICAGMAMAEKVVLYNLATL 483

Query: 488 LHAFEWKLSEGSKLDLEEKFGIVTKKLNPLVVIPMPRLSNLELY 523
           LH+F+W++ EG K++LEEKFGI+ K  NPLV  P+ RLS+  L+
Sbjct: 484 LHSFDWRIGEGEKVELEEKFGILLKLKNPLVATPVLRLSDPNLF 517

BLAST of Lsi09G003830 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 418.3 bits (1074), Expect = 9.0e-117
Identity = 218/510 (42.75%), Postives = 329/510 (64.51%), Query Frame = 0

Query: 20  EQISTALLFIFVFVVA-LLWLRPKFRR--PSLPPGPRGLPLVGYLPFLSRNLHHTFANLA 79
           +Q+   L  I +  +   LWL  K +R  P LPPGP GLP++G LPFL   LH  F  LA
Sbjct: 15  DQLDYGLTVIVISTLCWCLWLYAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLA 74

Query: 80  EIYGPIFKLRLGTKLCVVLTSPSSINEALRHQETVFANRDSTVSALLATYGGVDIVFSQD 139
           + +GPIFKL LG KL +V+TS     E L+  + +FAN D      + TYGG +I++S  
Sbjct: 75  KKHGPIFKLWLGAKLTIVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPY 134

Query: 140 DGDWKKLRKIFARKMLSKSNLDASYPLRRQEVRKVIKGVFESG--GNPVDIGKLGFFATV 199
              W+ LRK+   ++L  + LD+S  LRR+E R+ ++ + +    G+PV++G+  F   +
Sbjct: 135 GPKWRMLRKLCVNRILRNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMML 194

Query: 200 KSVMAMTWGGSGGLIGADGTDLEAKFREVVDELMVLVGSPNLSDLFPVLGRFDLQGIARK 259
             V  M WG +  +   +   + A+F EV+ E+  L+  PN+SD FPVL RFDLQG+A++
Sbjct: 195 NVVTQMLWGTT--VKEEEREVVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKR 254

Query: 260 MKKVMNVCDEILNSAIEEQRKVGENGAERRG--FLQFLLEVKDSKDSSESITDNQLKALL 319
           M++     D++ +  I ++  +  + ++ R   FL  LL+VKD +     +T N +KA+L
Sbjct: 255 MRRPAQRMDQMFDRIINQRLGMDRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVKAVL 314

Query: 320 MDIIVGGTDSTSTTIEWTITELIQQPNTMKKVVEELTKVVGLNQMVEEFHLSKLHYLDAV 379
           MD+++GGTD++   IE+ + EL+  P+ MK+  +E+ KVVG  ++VEE H+SKL Y+ A+
Sbjct: 315 MDMVLGGTDTSLHVIEFAMAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYILAI 374

Query: 380 IKETLRLHPPLPLLVPRTPSQTSTLGGYTIPKGSAIYFNIWAIQRDPKVWDKPLDFMPER 439
           +KETLRLH   PLLVPR PSQT+ +GG+TIPK S I+ N WAI R+P VW+ PL F P+R
Sbjct: 375 MKETLRLHTVAPLLVPRRPSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFDPDR 434

Query: 440 FLNESTEKVYDFTGNNIEFCPFGSGKKLCAGIPLAERLLVLILASLLHAFEWKLSEGSKL 499
           FL+ S    YDF GN+  + PFGSG+++C G+ + ER+++  LA+ LH+F+WK+ +G ++
Sbjct: 435 FLDMS----YDFKGNDFNYLPFGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQGERV 494

Query: 500 DLEEKFGIVTKKLNPLVVIPMPRLSNLELY 523
           ++EEKFGIV +  NPLV  P+ RLS+  LY
Sbjct: 495 EVEEKFGIVLELKNPLVATPVLRLSDPNLY 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SBQ97.0e-9036.76Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
A0A4D6Q4151.1e-8737.82Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
A0A4D6Q4143.3e-8735.36Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 G... [more]
A0A2K9RG087.3e-8736.83Labd-13Z-ene-9,15,16-triol synthase, chloroplastic OS=Vitex agnus-castus OX=5447... [more]
Q9SD851.2e-8636.76Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3ATA16.1e-24681.247-ethoxycoumarin O-deethylase-like OS=Cucumis melo OX=3656 GN=LOC103482652 PE=3 ... [more]
A0A5A7TDF04.0e-24581.047-ethoxycoumarin O-deethylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5A7T9J19.8e-24481.03Flavonoid 3'-monooxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3ATW04.8e-24381.03flavonoid 3'-monooxygenase-like OS=Cucumis melo OX=3656 GN=LOC103482653 PE=3 SV=... [more]
A0A6J1DW759.5e-20771.027-ethoxycoumarin O-deethylase-like OS=Momordica charantia OX=3673 GN=LOC11102367... [more]
Match NameE-valueIdentityDescription
XP_038906861.12.5e-25785.33labd-13Z-ene-9,15,16-triol synthase, chloroplastic-like [Benincasa hispida][more]
XP_004147610.21.9e-24681.84flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KAE8647886.1 hypothetica... [more]
XP_008437141.11.3e-24581.24PREDICTED: 7-ethoxycoumarin O-deethylase-like [Cucumis melo][more]
KAA0039671.18.2e-24581.047-ethoxycoumarin O-deethylase-like [Cucumis melo var. makuwa][more]
KAA0039673.12.0e-24381.03flavonoid 3'-monooxygenase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT4G12300.11.3e-13147.31cytochrome P450, family 706, subfamily A, polypeptide 4 [more]
AT4G12320.11.7e-13146.83cytochrome P450, family 706, subfamily A, polypeptide 6 [more]
AT4G12310.19.3e-13046.63cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT4G12330.12.6e-12444.27cytochrome P450, family 706, subfamily A, polypeptide 7 [more]
AT5G44620.19.0e-11742.75cytochrome P450, family 706, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 461..472
score: 35.25
coord: 318..335
score: 32.94
coord: 371..382
score: 52.33
coord: 452..461
score: 47.18
IPR001128Cytochrome P450PFAMPF00067p450coord: 49..506
e-value: 5.3E-102
score: 342.0
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 76..95
score: 40.6
coord: 461..484
score: 37.31
coord: 370..388
score: 44.87
coord: 307..324
score: 28.51
coord: 451..461
score: 48.38
coord: 411..435
score: 41.65
coord: 327..353
score: 30.18
coord: 100..121
score: 25.81
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 42..519
e-value: 1.3E-117
score: 395.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 49..515
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 26..486
e-value: 7.7E-23
score: 79.0
NoneNo IPR availablePANTHERPTHR47951OS08G0547900 PROTEINcoord: 10..523
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 454..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G003830.1Lsi09G003830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen