Lsi09G003540 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G003540
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Phytocyanin domain-containing protein
Location	chr09: 3691472 .. 3692544 (-)
RNA-Seq Expression	Lsi09G003540
Synteny	Lsi09G003540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTCTCCACCTCTCATCTCTTCGTCTTCCTCTCTCTTGCCGCGGTCATTTTTGCCCCTTCGGCTCTGGCCACCAATTACACCGTCGGAGATGATTCTGGTTGGAACATCGATGTTAACTATACATTGTGGGCTCAGGATAAAACATTCTACGTCAACGATGTTCTTAGTGAGTCATTCTATTCAACTCTATTAAGTTATTCATGTTTTTTTAAAAAAGAATGTGATTAAAAAGATTATATATACAAACAATAATTTCCAATAATTATAACCTCATTTTTAAATAAAGTTTGCAATAGTCTAACATGATATCAAAATAAAATGTCATATACCTAATAGATCATACAATAGTGATCTATAGATGGGCCTTAAGAATCGCATATGTACCCGCATTAACTTTTTGACATGCATACGATTTATTGAACATATATATTTGTACTTTTTTTCTAATATTTGGGGGCGTGTCGTCGGTTACGTTGTAGTCATTTTTTTAGCCACTTCACAACACAACGGAAAAACTAAAAAATATATATATTATAAAGGAGTAATACATAGGTGCAACTCATATTATATGTTGTGCAGTTCAAAATGCACCAAAGTATTTTTCATTATAGAAAAAAAGAAAAACGAAAAGTTTTTCACGTGTAAAAATAGAGTTAACAATAGGAAGCACCTAAGTATTGTCGTGGATTTTTCACGAATAAAATGGGTTTTTTTGTTTTTGTTTAGTTTTCAAGTACCCAGCAGGAGACCACAATGTGTACAAGGTCAATGGCAGTGATTTCCACAATTGCACAGTACCAGCTGGCCAGACTCCATTGACGACTGGAAACGATGTCATTGTATTGGCAAAGTCTGGAAGAAAATGGTACATCTGTGGAAAAGAAGGCCACTGTGGGAAAAGCCAGAAGCTGGTAATTACGGTCATGGATATGGCTCCAGCCGCCAATTCACCACTTCCCGGTGCAACCCCGCCACCGCCACCATCTGCAGCCACCAAACCGGTTGTTTCCGCCCAATTTGGGCTCGTCGCATTGGTGCTTGCCGTCCTTGGGATGATGATGGCTTAG

mRNA sequence

ATGGCCTTCTCCACCTCTCATCTCTTCGTCTTCCTCTCTCTTGCCGCGGTCATTTTTGCCCCTTCGGCTCTGGCCACCAATTACACCGTCGGAGATGATTCTGGTTGGAACATCGATGTTAACTATACATTGTGGGCTCAGGATAAAACATTCTACGTCAACGATGTTCTTATTTTCAAGTACCCAGCAGGAGACCACAATGTGTACAAGGTCAATGGCAGTGATTTCCACAATTGCACAGTACCAGCTGGCCAGACTCCATTGACGACTGGAAACGATGTCATTGTATTGGCAAAGTCTGGAAGAAAATGGTACATCTGTGGAAAAGAAGGCCACTGTGGGAAAAGCCAGAAGCTGGTAATTACGGTCATGGATATGGCTCCAGCCGCCAATTCACCACTTCCCGGTGCAACCCCGCCACCGCCACCATCTGCAGCCACCAAACCGGTTGTTTCCGCCCAATTTGGGCTCGTCGCATTGGTGCTTGCCGTCCTTGGGATGATGATGGCTTAG

Coding sequence (CDS)

ATGGCCTTCTCCACCTCTCATCTCTTCGTCTTCCTCTCTCTTGCCGCGGTCATTTTTGCCCCTTCGGCTCTGGCCACCAATTACACCGTCGGAGATGATTCTGGTTGGAACATCGATGTTAACTATACATTGTGGGCTCAGGATAAAACATTCTACGTCAACGATGTTCTTATTTTCAAGTACCCAGCAGGAGACCACAATGTGTACAAGGTCAATGGCAGTGATTTCCACAATTGCACAGTACCAGCTGGCCAGACTCCATTGACGACTGGAAACGATGTCATTGTATTGGCAAAGTCTGGAAGAAAATGGTACATCTGTGGAAAAGAAGGCCACTGTGGGAAAAGCCAGAAGCTGGTAATTACGGTCATGGATATGGCTCCAGCCGCCAATTCACCACTTCCCGGTGCAACCCCGCCACCGCCACCATCTGCAGCCACCAAACCGGTTGTTTCCGCCCAATTTGGGCTCGTCGCATTGGTGCTTGCCGTCCTTGGGATGATGATGGCTTAG

Protein sequence

MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA

Homology

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BLAST of Lsi09G003540 vs. ExPASy Swiss-Prot
Match: G7L0H3 (Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 7.4e-33
Identity = 75/169 (44.38%), Postives = 105/169 (62.13%), Query Frame = 0


Query: 4   STSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPA 63
           ++S + + LS++ V+ +  A+AT++ VGDD GW +D +YT WAQDK F V D L+F Y  
Sbjct: 2   ASSRVVLILSISMVLLSSVAIATDHIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYDP 61

Query: 64  GDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQ-KLVIT 123
             HNV+KVNG+ F +CT P     L+TG D+I L   GRKWY+CG   HC   Q KLVIT
Sbjct: 62  ARHNVFKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVIT 121

Query: 124 VM-DMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA 171
           V+ + APA         P PPPS+    VVS+ FG+V  ++  + ++ A
Sbjct: 122 VLAEGAPA---------PSPPPSSDAHSVVSSLFGVVMAIMVAIAVIFA 161

[Close]

BLAST of Lsi09G003540 vs. ExPASy Swiss-Prot
Match: A0A072U307 (Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.8e-32
Identity = 76/170 (44.71%), Postives = 105/170 (61.76%), Query Frame = 0


Query: 4   STSHLFVFLSLAAVIFAPSAL-ATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYP 63
           ++S + + LS++ V+ +  A+ AT+Y VGDD GW +D +YT WAQDK F V D L+F Y 
Sbjct: 2   ASSRVVLILSISMVLLSSVAIAATDYIVGDDKGWTVDFDYTQWAQDKVFRVGDNLVFNYD 61

Query: 64  AGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQ-KLVI 123
              HNV+KVNG+ F +CT P     L+TG D+I L   GRKWY+CG   HC   Q KLVI
Sbjct: 62  PSRHNVFKVNGTLFQSCTFPPKNEALSTGKDIIQLKTEGRKWYVCGVADHCSARQMKLVI 121

Query: 124 TVM-DMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA 171
           TV+ + APA         P PPPS+    VVS+ FG+V  ++  + ++ A
Sbjct: 122 TVLAEGAPA---------PSPPPSSDAHSVVSSLFGVVMAIMVAIAVIFA 162

[Close]

BLAST of Lsi09G003540 vs. ExPASy Swiss-Prot
Match: A0A0M4FTF3 (Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-28
Identity = 65/123 (52.85%), Postives = 76/123 (61.79%), Query Frame = 0


Query: 20  APSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKVNGSDFHNC 79
           A  A  T Y VGDD GW IDV+Y  WA+ KTF V D L+FKY  G HNV+KVN + F NC
Sbjct: 110 ASPAPQTEYWVGDDKGWTIDVDYQAWAKGKTFKVGDTLVFKYTKGHHNVFKVNQTGFQNC 169

Query: 80  TVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGK-SQKLVITVMDMAPAANSPLPGAT 139
             P     LT+G+DVI LA  G+KWYICG   HC +  QKL ITV + APA   P+    
Sbjct: 170 IAPPPSEGLTSGHDVITLAAPGKKWYICGFPTHCSEHKQKLAITV-EGAPAQTPPVWAPA 229

Query: 140 PPP 142
           P P
Sbjct: 230 PAP 231

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BLAST of Lsi09G003540 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.0e-21
Identity = 63/139 (45.32%), Postives = 81/139 (58.27%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MAFS + +  FL LA +  A  +LAT YTVGD SGW I  +Y+ WA DKTF V D L+F 
Sbjct: 1   MAFSNALVLCFL-LAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFN 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           Y AG H V +V  SD+ +CT     +  +TG   I L K+G+ ++ICG  GH     KL 
Sbjct: 61  YGAGAHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLS 120

Query: 121 ITVMDMAPAANSPLPGATP 140
           I V   A + +S  P ATP
Sbjct: 121 IKV--KASSGSSAAPSATP 136

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BLAST of Lsi09G003540 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 0


Query: 4   STSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPA 63
           ++  + + +S+ A       +AT++T+G  SGW +  +   WA  +TF V D L+F YPA
Sbjct: 2   ASREMLIIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPA 61

Query: 64  GDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITV 123
             H+V +V   +F +C           GN ++ L   G++++ICG  GHC +  KL + V
Sbjct: 62  AFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNV 121

Query: 124 MDMAPAA-NSPLPGATP---PPPPSA 146
           +  A  A  +PLP   P    P PS+
Sbjct: 122 VPTATVAPTAPLPNTVPSLNAPSPSS 147

[Close]

BLAST of Lsi09G003540 vs. ExPASy TrEMBL
Match: A0A1S3ATX0 (mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103482677 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.8e-66
Identity = 132/169 (78.11%), Postives = 143/169 (84.62%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLFV LS A V+ APSALATNYTVGDD+GW+I+VNYT+WAQ K F V D+LIF 
Sbjct: 1   MAISTSHLFVLLSFAVVMSAPSALATNYTVGDDAGWSINVNYTVWAQGKMFNVGDMLIFN 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           YP GDHNV+KVNGSDF +CTVP  Q  L TG+DVIVLAK GRKWYICGKEGHCG+ QKLV
Sbjct: 61  YPPGDHNVFKVNGSDFKSCTVPKDQNALATGSDVIVLAKPGRKWYICGKEGHCGQGQKLV 120

Query: 121 ITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMM 170
           I VMDM P ANSPLPG TPPPPPSAATK VVS QFG VALV+AVLGMMM
Sbjct: 121 IDVMDMGP-ANSPLPGGTPPPPPSAATKAVVSGQFGFVALVVAVLGMMM 168

[Close]

BLAST of Lsi09G003540 vs. ExPASy TrEMBL
Match: A0A6J1HYR4 (stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111468879 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.9e-66
Identity = 133/170 (78.24%), Postives = 145/170 (85.29%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLF+FLS+ A IFAPSALATNYTVGDD+GWN +VNYT WAQ KTFYV D LIFK
Sbjct: 1   MAISTSHLFLFLSIVA-IFAPSALATNYTVGDDAGWNTNVNYTAWAQGKTFYVGDFLIFK 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           YPAGDHNVYKVNGSDF NCTVPA QTP +TGND I LAKSG+KWYICGKEGHCG+SQKLV
Sbjct: 61  YPAGDHNVYKVNGSDFQNCTVPADQTPFSTGNDPIFLAKSGKKWYICGKEGHCGQSQKLV 120

Query: 121 ITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA 171
           ITVMDMAP ANSP      PPPPSAATK V+S Q G V +V+A+LG+MMA
Sbjct: 121 ITVMDMAP-ANSP-----APPPPSAATKAVLSGQIGFVVVVVAILGLMMA 163

[Close]

BLAST of Lsi09G003540 vs. ExPASy TrEMBL
Match: A0A6J1E3D3 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111430223 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.1e-63
Identity = 130/170 (76.47%), Postives = 142/170 (83.53%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLF+FLS+ A IFAP ALATNYTVGDD+GWN +VNYT WAQ KTFYV D LIFK
Sbjct: 1   MAISTSHLFLFLSIVA-IFAPPALATNYTVGDDAGWNTNVNYTAWAQGKTFYVGDFLIFK 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           Y AGDHNVYKVN SDF NCTVPA QTP +TGND I LAKSG+KWYICGK+GHCG+SQKLV
Sbjct: 61  YTAGDHNVYKVNASDFQNCTVPADQTPFSTGNDPIFLAKSGKKWYICGKDGHCGQSQKLV 120

Query: 121 ITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA 171
           ITVMDMAP ANSP      PPPPSAATK V+S Q G V +V+A+LGMMMA
Sbjct: 121 ITVMDMAP-ANSP-----APPPPSAATKAVLSGQIGFVVVVVAILGMMMA 163

[Close]

BLAST of Lsi09G003540 vs. ExPASy TrEMBL
Match: A0A0A0KKF3 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G157340 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.3e-61
Identity = 122/157 (77.71%), Postives = 136/157 (86.62%), Query Frame = 0


Query: 14  LAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKVNG 73
           +A V++APSALATNYTVGDD+GW+I+VNYTLWAQ K F V D+LIF YP GDHNV+KVNG
Sbjct: 1   MAVVMYAPSALATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFNYPPGDHNVFKVNG 60

Query: 74  SDFHNCTVPA-GQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITVMDMAPAANS 133
           SDF NCT+P  GQ  LT+G+DVIVLAK G+KWYICGKEGHCG+ QKLVI VMDM P ANS
Sbjct: 61  SDFQNCTLPKDGQNALTSGSDVIVLAKPGKKWYICGKEGHCGQGQKLVINVMDMGP-ANS 120

Query: 134 PLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMM 170
           PLPG T PPPPSAATK VVSAQFG VALV+AVLGMMM
Sbjct: 121 PLPGGTAPPPPSAATKAVVSAQFGFVALVVAVLGMMM 156

[Close]

BLAST of Lsi09G003540 vs. ExPASy TrEMBL
Match: A0A5A7THF2 (Mavicyanin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003410 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-60
Identity = 119/150 (79.33%), Postives = 129/150 (86.00%), Query Frame = 0


Query: 20  APSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKVNGSDFHNC 79
           APSALATNYTVGDD+GW+I+VNYT+WAQ K F V D+LIF YP GDHNV+KVNGSDF +C
Sbjct: 3   APSALATNYTVGDDAGWSINVNYTVWAQGKMFNVGDMLIFNYPPGDHNVFKVNGSDFKSC 62

Query: 80  TVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITVMDMAPAANSPLPGATP 139
           TVP  Q  L TG+DVIVLAK GRKWYICGKEGHCG+ QKLVI VMDM P ANSPLPG TP
Sbjct: 63  TVPKDQNALATGSDVIVLAKPGRKWYICGKEGHCGQGQKLVIDVMDMGP-ANSPLPGGTP 122

Query: 140 PPPPSAATKPVVSAQFGLVALVLAVLGMMM 170
           PPPPSAATK VVS QFG VALV+AVLGMMM
Sbjct: 123 PPPPSAATKAVVSVQFGFVALVVAVLGMMM 151

[Close]

BLAST of Lsi09G003540 vs. NCBI nr
Match: XP_038874887.1 (blue copper protein 1a-like [Benincasa hispida])

HSP 1 Score: 270.0 bits (689), Expect = 1.4e-68
Identity = 135/170 (79.41%), Postives = 148/170 (87.06%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLFVFLS+AAV FAPSALATNYTVGDD+GWN+ VNYTLWA DK F V D+LIF 
Sbjct: 1   MAISTSHLFVFLSIAAVAFAPSALATNYTVGDDAGWNVGVNYTLWANDKMFNVGDILIFN 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPA-GQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKL 120
           YP GDHNV+KVNGSDFH+C+VP  GQ  LTTGND IVLAK G+KWYICGKEGHCGK QKL
Sbjct: 61  YPVGDHNVFKVNGSDFHDCSVPKDGQNALTTGNDAIVLAKPGKKWYICGKEGHCGKGQKL 120

Query: 121 VITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMM 170
           VITVM+MAP ANSPLPG + PPPPSAATK VVSA FG +AL++AVLGMMM
Sbjct: 121 VITVMNMAP-ANSPLPGGSAPPPPSAATKAVVSAHFGFLALLVAVLGMMM 169

[Close]

BLAST of Lsi09G003540 vs. NCBI nr
Match: XP_004147603.1 (mavicyanin [Cucumis sativus] >KAE8647880.1 hypothetical protein Csa_000472 [Cucumis sativus])

HSP 1 Score: 263.1 bits (671), Expect = 1.7e-66
Identity = 133/170 (78.24%), Postives = 147/170 (86.47%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLFV LS+A V++APSALATNYTVGDD+GW+I+VNYTLWAQ K F V D+LIF 
Sbjct: 1   MAISTSHLFVLLSMAVVMYAPSALATNYTVGDDAGWSINVNYTLWAQGKMFNVGDMLIFN 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPA-GQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKL 120
           YP GDHNV+KVNGSDF NCT+P  GQ  LT+G+DVIVLAK G+KWYICGKEGHCG+ QKL
Sbjct: 61  YPPGDHNVFKVNGSDFQNCTLPKDGQNALTSGSDVIVLAKPGKKWYICGKEGHCGQGQKL 120

Query: 121 VITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMM 170
           VI VMDM P ANSPLPG T PPPPSAATK VVSAQFG VALV+AVLGMMM
Sbjct: 121 VINVMDMGP-ANSPLPGGTAPPPPSAATKAVVSAQFGFVALVVAVLGMMM 169

[Close]

BLAST of Lsi09G003540 vs. NCBI nr
Match: XP_008437169.1 (PREDICTED: mavicyanin-like [Cucumis melo])

HSP 1 Score: 261.9 bits (668), Expect = 3.8e-66
Identity = 132/169 (78.11%), Postives = 143/169 (84.62%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLFV LS A V+ APSALATNYTVGDD+GW+I+VNYT+WAQ K F V D+LIF 
Sbjct: 1   MAISTSHLFVLLSFAVVMSAPSALATNYTVGDDAGWSINVNYTVWAQGKMFNVGDMLIFN 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           YP GDHNV+KVNGSDF +CTVP  Q  L TG+DVIVLAK GRKWYICGKEGHCG+ QKLV
Sbjct: 61  YPPGDHNVFKVNGSDFKSCTVPKDQNALATGSDVIVLAKPGRKWYICGKEGHCGQGQKLV 120

Query: 121 ITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMM 170
           I VMDM P ANSPLPG TPPPPPSAATK VVS QFG VALV+AVLGMMM
Sbjct: 121 IDVMDMGP-ANSPLPGGTPPPPPSAATKAVVSGQFGFVALVVAVLGMMM 168

[Close]

BLAST of Lsi09G003540 vs. NCBI nr
Match: XP_022969776.1 (stellacyanin-like [Cucurbita maxima])

HSP 1 Score: 260.0 bits (663), Expect = 1.4e-65
Identity = 133/170 (78.24%), Postives = 145/170 (85.29%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA STSHLF+FLS+ A IFAPSALATNYTVGDD+GWN +VNYT WAQ KTFYV D LIFK
Sbjct: 1   MAISTSHLFLFLSIVA-IFAPSALATNYTVGDDAGWNTNVNYTAWAQGKTFYVGDFLIFK 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           YPAGDHNVYKVNGSDF NCTVPA QTP +TGND I LAKSG+KWYICGKEGHCG+SQKLV
Sbjct: 61  YPAGDHNVYKVNGSDFQNCTVPADQTPFSTGNDPIFLAKSGKKWYICGKEGHCGQSQKLV 120

Query: 121 ITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA 171
           ITVMDMAP ANSP      PPPPSAATK V+S Q G V +V+A+LG+MMA
Sbjct: 121 ITVMDMAP-ANSP-----APPPPSAATKAVLSGQIGFVVVVVAILGLMMA 163

[Close]

BLAST of Lsi09G003540 vs. NCBI nr
Match: XP_023550536.1 (stellacyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.8 bits (652), Expect = 2.7e-64
Identity = 131/170 (77.06%), Postives = 143/170 (84.12%), Query Frame = 0


Query: 1   MAFSTSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFK 60
           MA S SHLF+FLS+ + IFAPSALATNYTVGDD+GWN +VNYT WAQ KTFYV D LIFK
Sbjct: 1   MAISASHLFLFLSIVS-IFAPSALATNYTVGDDAGWNTNVNYTAWAQGKTFYVGDFLIFK 60

Query: 61  YPAGDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLV 120
           YPAGDHNVYKVN SDF NCTVPA QTP +TGND I LAKSG+KWYICGK+GHCG+SQKLV
Sbjct: 61  YPAGDHNVYKVNASDFQNCTVPADQTPFSTGNDPIFLAKSGKKWYICGKDGHCGQSQKLV 120

Query: 121 ITVMDMAPAANSPLPGATPPPPPSAATKPVVSAQFGLVALVLAVLGMMMA 171
           ITVMDMAP ANSP      PPPPSAATK V+S Q G V +VLA+LGMMMA
Sbjct: 121 ITVMDMAP-ANSP-----APPPPSAATKAVLSGQIGFVVVVLAILGMMMA 163

[Close]

BLAST of Lsi09G003540 vs. TAIR 10
Match: AT3G17675.1 (Cupredoxin superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 6.1e-22
Identity = 43/98 (43.88%), Postives = 61/98 (62.24%), Query Frame = 0


Query: 26  TNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKVNGSDFHNCTVPAGQ 85
           T + VGD +GW +  NYT W Q + F+V DVL+F Y +  HNV +VN + + +C +    
Sbjct: 7   TEHIVGDSNGWELFTNYTNWTQGREFHVGDVLVFNYKSDQHNVMQVNSTAYTDCGLDNYT 66

Query: 86  TPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITV 124
           T  T GND I+L++ G+ W+ICG + HC   QKL I V
Sbjct: 67  TLFTKGNDSIILSEVGKLWFICGVDDHCVNGQKLSINV 104

[Close]

BLAST of Lsi09G003540 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 94.0 bits (232), Expect = 1.3e-19
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 0


Query: 4   STSHLFVFLSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPA 63
           ++  + + +S+ A       +AT++T+G  SGW +  +   WA  +TF V D L+F YPA
Sbjct: 2   ASREMLIIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPA 61

Query: 64  GDHNVYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITV 123
             H+V +V   +F +C           GN ++ L   G++++ICG  GHC +  KL + V
Sbjct: 62  AFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNV 121

Query: 124 MDMAPAA-NSPLPGATP---PPPPSA 146
           +  A  A  +PLP   P    P PS+
Sbjct: 122 VPTATVAPTAPLPNTVPSLNAPSPSS 147

[Close]

BLAST of Lsi09G003540 vs. TAIR 10
Match: AT2G31050.1 (Cupredoxin superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 2.8e-19
Identity = 56/144 (38.89%), Postives = 76/144 (52.78%), Query Frame = 0


Query: 9   FVFLSLAAVIFAPSALATNYTVGDDSGWNI-DVNYTLWAQDKTFYVNDVLIFKYPAGDHN 68
           F  L +   +F  S   T + VGD  GW I  VNY  WA   TF V D L+FKY    H+
Sbjct: 10  FTSLLILVALFGISVGGTVHKVGDSDGWTIMSVNYETWASTITFQVGDSLVFKYNKDFHD 69

Query: 69  VYKVNGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITVM--D 128
           V +V  +D+  C          TG+D+++L K G + +ICG  GHC   QKL I V+   
Sbjct: 70  VTEVTHNDYEMCEPSKPLARYETGSDIVILTKPGLQHFICGFPGHCDMGQKLQIHVLPAS 129

Query: 129 MAPAANSPLPGATPPPPPSAATKP 150
           + P A +P+PG  P  PPS+ + P
Sbjct: 130 LGPVA-APVPG--PVRPPSSFSSP 150

[Close]

BLAST of Lsi09G003540 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 2.4e-18
Identity = 48/127 (37.80%), Postives = 71/127 (55.91%), Query Frame = 0


Query: 25  ATNYTVGDDSGWN--IDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKVNGSDFHNCTVP 84
           A  Y VGD +GW    +V+Y LWA  KTF++ D ++F+Y    HNV +V    + +C   
Sbjct: 21  AAVYKVGDSAGWTTIANVDYKLWASTKTFHIGDTVLFEYNPQFHNVMRVTHPMYRSCNTS 80

Query: 85  AGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITVMDMAPAANSPLPGATPPPP 144
              +  TTGND I L   G  ++ CG  GHC   QKL + V  + PA+++PL   + PP 
Sbjct: 81  KPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLHV--LLPASSTPL---SDPPT 140

Query: 145 PSAATKP 150
            S+++ P
Sbjct: 141 SSSSSPP 142

[Close]

BLAST of Lsi09G003540 vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-17
Identity = 50/145 (34.48%), Postives = 69/145 (47.59%), Query Frame = 0


Query: 12  LSLAAVIFAPSALATNYTVGDDSGWNIDVNYTLWAQDKTFYVNDVLIFKYPAGDHNVYKV 71
           L L  ++  P+  A  + VGD+ GW I V YT W  +KTF V D L FKY    H+V  V
Sbjct: 10  LLLLLLVAVPAVFAVTFQVGDNDGWTIGVEYTSWVSEKTFRVGDTLEFKY-GPSHSVAVV 69

Query: 72  NGSDFHNCTVPAGQTPLTTGNDVIVLAKSGRKWYICGKEGHCGKSQKLVITVM------- 131
           N +D+  C         + G+  I L K G   ++C   GHC    KL + V+       
Sbjct: 70  NKADYDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCSLGMKLAVQVLAAVSLEP 129

Query: 132 DMAPAANSPLPGATPPPPPSAATKP 150
             +P+A SP P A  P P + +  P
Sbjct: 130 PPSPSAPSPSPSAPSPSPSAPSPSP 153

The following BLAST results are available for this feature:

BLAST of Lsi09G003540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7L0H3	7.4e-33	44.38	Blue copper protein 1a OS=Medicago truncatula OX=3880 GN=MTR_7g086140 PE=2 SV=1	[more]
A0A072U307	4.8e-32	44.71	Blue copper protein 1b OS=Medicago truncatula OX=3880 GN=BCP1B PE=2 SV=1	[more]
A0A0M4FTF3	1.1e-28	52.85	Blue copper protein OS=Petunia hybrida OX=4102 GN=BCP PE=2 SV=1	[more]
Q41001	1.0e-21	45.32	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
O82081	1.8e-18	32.19	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]

BLAST of Lsi09G003540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3ATX0	1.8e-66	78.11	mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103482677 PE=4 SV=1	[more]
A0A6J1HYR4	6.9e-66	78.24	stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111468879 PE=4 SV=1	[more]
A0A6J1E3D3	1.1e-63	76.47	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111430223 PE=4 SV=1	[more]
A0A0A0KKF3	2.3e-61	77.71	Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G157340...	[more]
A0A5A7THF2	1.5e-60	79.33	Mavicyanin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G00341...	[more]

BLAST of Lsi09G003540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038874887.1	1.4e-68	79.41	blue copper protein 1a-like [Benincasa hispida]	[more]
XP_004147603.1	1.7e-66	78.24	mavicyanin [Cucumis sativus] >KAE8647880.1 hypothetical protein Csa_000472 [Cucu...	[more]
XP_008437169.1	3.8e-66	78.11	PREDICTED: mavicyanin-like [Cucumis melo]	[more]
XP_022969776.1	1.4e-65	78.24	stellacyanin-like [Cucurbita maxima]	[more]
XP_023550536.1	2.7e-64	77.06	stellacyanin-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi09G003540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17675.1	6.1e-22	43.88	Cupredoxin superfamily protein	[more]
AT2G32300.1	1.3e-19	32.19	uclacyanin 1	[more]
AT2G31050.1	2.8e-19	38.89	Cupredoxin superfamily protein	[more]
AT5G26330.1	2.4e-18	37.80	Cupredoxin superfamily protein	[more]
AT3G60270.1	1.5e-17	34.48	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 37..115 e-value: 1.4E-21 score: 76.3
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 26..125 score: 37.292198
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 24..128 e-value: 9.5E-34 score: 117.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 25..124
None	No IPR available	PANTHER	PTHR33021:SF411	BLUE COPPER PROTEIN-LIKE	coord: 8..135
None	No IPR available	CDD	cd04216	Phytocyanin	coord: 26..123 e-value: 3.04256E-41 score: 131.999
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 8..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G003540.1	Lsi09G003540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity