Lsi09G002270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G002270
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Beta-carotene hydroxylase
Location	chr09: 2375121 .. 2378233 (+)
RNA-Seq Expression	Lsi09G002270
Synteny	Lsi09G002270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCCCTCAAATTCCATTTTATAAATTATACCCTGAATTTCTTTCTTCAAGTTGTATCCATTTCTTTCATGGCGACGGTGATTTCGGCCGTTGCAACGGCCGCGCCGTACCAATTTGATTACCGGAGATTGTTTCAACGGCCGGCCGGAGCTCCGTCTCTACGGCTGGGTTCTCGGAAGCGTTTTCCATGGCGGTGTTTTGCGAAACAGAGCTCGAGAAGATTGCTGGCTATGGAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAGATGACTGTGGGGGAGAAAATGGCGAGGAAGAAAGCGGAAAGGGATGCTTATTTGATAGCTGCAATAGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACAGATTCTCTTGGCAAATGGGGGTACTCTGTTTTCATCTTTTTTTTCTTTTAAAGAAATTTCATTAACATCCATCGACTTCGTTTTTTAATTCAATACTAATTTAATGATTATGAGCATATTGTTGATTAGTAAGTTTTTGTGATATGAAATCTTCAATAAGATTTTTCTTAAATGGAGGATTCACCCAAAAGTTGCTTGGACACATGACATATTGGAGTAACTTGCTCTGATACCATCATCTACCTAAAAGCTTAAGCTAATAGGTGAAGGCAAATTTAATATAATATCTAACACTCTTCCTCACATGTCAGCAATCTGCTCTGATACCATCTTAAATCGTCGATTCACCCAAAAGTTTAAGTGAATGTAAATTTAATATAATATCTAACAATTTTGTCTTTTAAAATTAAAAAAACTAACTACGTTATTAAATTATTGTTATATAAAAAATATTTAGATGTATCAAAAGTTGTCCTTAGTAACTTAGAATGTACTCAATGTTAAAGTATTTGGACATTCTATCAAAGACATGTCAGTAGAAAAAAAATGAACTTTTGTTTGATTATGTGGATGTAACACCATTTTTAAATGTAGTTATGGTATGAAATTTTTATTAGTACTAAAAATTATTTTCATGTGTTCAAATATATTAATAAAGTGACATAAAGAAGTGATTTGAAAATTTTAAATTATAGATAAATTTTTATATTTATGTTTAAGTTTTTAGGTTAACAAGTTTTAAAAGGAAGGAGCTGAATATTACAATTCATTTACAGGGTAGAGAGTTTCCAGTTTTGGAAATGTTCGGCACTTTTGCATTATCCATCGGCGCTGCTGTGAGTTGAAACTTTAAATGAATTAAAGGAGTTCATTCGGAACGACTTTCAAAATGTTTATTTTTCTAAAAAATTTAAAAAGTCATTCCAAACAAATTCTAAAATATTTTTCATTTATCTTATTTGGAGAATTTGATGGGTTATATTAAGGTGGGAATGGAATTTTGGGCAAGATGGGCTCACAAAGAGCTATGGCATGCTTCGTTGTGGGATATGCATGAGGTAATAAGGTGTTACATTAAATGTGTAAATAATGAAGATAATTTAAAATATAATATAATGTTGAAAAGCATATGGTAAAAATTAATTTGTAAATTTTTTTTTTTTGGGTAGTCTCATCACCGGCCGAGGGTTGGAGCTTTTGAATTCAACGATGTGTTTGCAATTATCAACGCTGTTCCAGCCATTGCTCTTCTTTCTTTTGGCTTCTTCAATCAAGGCTTCTTTCCCGGCCTCTGTTTTGGTGCCGTACGTCTCTTTCTCTCTCTATTTATTATTATTTTTTATTTTAGTCCCTTAAATTTTAAAATGACTGTTTTAATCTTAAACTTAAAAAAAAAAAAAAAACAATTCTTTTGGTTGTTATTTTAAAATTGTTTTCAAATATAAAAAAAATAACAAAATTATTTACAAATGTAGAAAATTTTTACTATTTATCGTTCAAACGATAGAATTCTATCGCAGTTTATCGTTGATAGACTAATACAATTTTTCCATATTTATAAATAGTTTAATGTTTTTTTTGTTTATAGTAACTTATCTTAATTTATTTTATTTTTGACAAACTAATGTTGTAATTTTTTTTCGTACGTGTACATTTGTTCATATGAACGTGATTAAAAATGAACATAGAGTAAGTGAAAATTAGATGAAGAAAGGTAATTTAACATGAGCCAAAATGATTGCTTTTCAGTTAGCATGAGCGTAGTTGAACAATAATTGGTATGTAATTTTTTTTTAAAAGGTTTGAGGTTCAAATCTTTATACCTTTACATTTGTTGTACTACAAGAAATAGTTGTTTTTAAAAAGTATTGAGACTAAAATAGATATTATAAAAAATTTAGAGACTGAAATGAAATCGACTTAAAAGTTTGGAGACTAAAATGAGATTTAAACTCTTTACTTTTAAATACATTGTTGGAATTAAAATCAGAAGACTAATAACATAATTAAAAAAAAAATTTAAAAATAAAATCATATATTTTTTGGTTTTTAATTTTTAAAAATTAAGTTTATAAATACTATTTTCACCTCTCAATTTCAAGTTTGTGTTATCTACTTTATATAGAATTGATTTCAAAAATCAAGCTAGATTTTAGAAACTAAAACAAGTTTAAGTTTTTATTTTTATTTTTATTTTTATTTTTGAAGAATTTGATTAAAAACTTAAATGTTTCTTGAAGAATATGAAAATTATAATAAGAAAATTTGAGAAAAGAAAGCATAAAATAAAACTAAAAACAAAATCCTAAATATCATTTTCATCTAAATCTAGAAATGAATTTGTAATCTATCTAAAAAAAATGTTATTTAAGATTGAATCCTTATTTTTTCTTATAAATAATAAGATCCAATATTTAAAGTATTTCTTCCTAATTTTATGAAAAATGATGGATGATTCATATTTTTCAACTTTAGAATCCTTCCAACCCATTGAAGTCCAAGTCATTGTATGACGTTGCGTTCTATGATTGGCCATTGACCGTTATTACTAATTTTCAACAGGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCACGATGGCCTCGTGCACTGTCGTTTCCCAGTGGGTCCCATAGCCGCTGTCCCTTACTTACGACGTGTCGCTGCTGCCCATCATGTATTTATCGACGTGTCGTCGGTTTTTGCCTAA

mRNA sequence

CCCCCTCAAATTCCATTTTATAAATTATACCCTGAATTTCTTTCTTCAAGTTGTATCCATTTCTTTCATGGCGACGGTGATTTCGGCCGTTGCAACGGCCGCGCCGTACCAATTTGATTACCGGAGATTGTTTCAACGGCCGGCCGGAGCTCCGTCTCTACGGCTGGGTTCTCGGAAGCGTTTTCCATGGCGGTGTTTTGCGAAACAGAGCTCGAGAAGATTGCTGGCTATGGAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAGATGACTGTGGGGGAGAAAATGGCGAGGAAGAAAGCGGAAAGGGATGCTTATTTGATAGCTGCAATAGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACAGATTCTCTTGGCAAATGGGGGTGGGAATGGAATTTTGGGCAAGATGGGCTCACAAAGAGCTATGGCATGCTTCGTTGTGGGATATGCATGAGTCTCATCACCGGCCGAGGGTTGGAGCTTTTGAATTCAACGATGTGTTTGCAATTATCAACGCTGTTCCAGCCATTGCTCTTCTTTCTTTTGGCTTCTTCAATCAAGGCTTCTTTCCCGGCCTCTGTTTTGGTGCCAATCCTTCCAACCCATTGAAGTCCAAGTCATTGTATGACGTTGCGTTCTATGATTGGCCATTGACCGTTATTACTAATTTTCAACAGGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCACGATGGCCTCGTGCACTGTCGTTTCCCAGTGGGTCCCATAGCCGCTGTCCCTTACTTACGACGTGTCGCTGCTGCCCATCATGTATTTATCGACGTGTCGTCGGTTTTTGCCTAA

Coding sequence (CDS)

ATGGCGACGGTGATTTCGGCCGTTGCAACGGCCGCGCCGTACCAATTTGATTACCGGAGATTGTTTCAACGGCCGGCCGGAGCTCCGTCTCTACGGCTGGGTTCTCGGAAGCGTTTTCCATGGCGGTGTTTTGCGAAACAGAGCTCGAGAAGATTGCTGGCTATGGAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAGATGACTGTGGGGGAGAAAATGGCGAGGAAGAAAGCGGAAAGGGATGCTTATTTGATAGCTGCAATAGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACAGATTCTCTTGGCAAATGGGGGTGGGAATGGAATTTTGGGCAAGATGGGCTCACAAAGAGCTATGGCATGCTTCGTTGTGGGATATGCATGAGTCTCATCACCGGCCGAGGGTTGGAGCTTTTGAATTCAACGATGTGTTTGCAATTATCAACGCTGTTCCAGCCATTGCTCTTCTTTCTTTTGGCTTCTTCAATCAAGGCTTCTTTCCCGGCCTCTGTTTTGGTGCCAATCCTTCCAACCCATTGAAGTCCAAGTCATTGTATGACGTTGCGTTCTATGATTGGCCATTGACCGTTATTACTAATTTTCAACAGGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCACGATGGCCTCGTGCACTGTCGTTTCCCAGTGGGTCCCATAGCCGCTGTCCCTTACTTACGACGTGTCGCTGCTGCCCATCATGTATTTATCGACGTGTCGTCGGTTTTTGCCTAA

Protein sequence

MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQMGVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHVFIDVSSVFA

Homology

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BLAST of Lsi09G002270 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-60
Identity = 128/221 (57.92%), Postives = 146/221 (66.06%), Query Frame = 0


Query: 54  AMEKKKSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQ 113
           A E +K  E E+    +  + +K+ARKK+ER  YL+AA++SSFGITSMA ++VYYRFSWQ
Sbjct: 86  AGEVRKQREKEISASAE-KLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQ 145

Query: 114 M--------------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFND 173
           M                     VGMEFWARWAH+ LWHASLW MHESHH+PR G FE ND
Sbjct: 146 MEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPFELND 205

Query: 174 VFAIINAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQ 233
           +FAIINAVPAIALLS+GFF++G  PGLCFGA                             
Sbjct: 206 IFAIINAVPAIALLSYGFFHKGLIPGLCFGA----------------------------- 265

Query: 234 GLGITVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAH 255
           GLGITVFGMAYMFVHDGLVH RFPVGPIA VPY RRVAAAH
Sbjct: 266 GLGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAH 276

[Close]

BLAST of Lsi09G002270 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.9e-60
Identity = 135/255 (52.94%), Postives = 158/255 (61.96%), Query Frame = 0


Query: 28  APSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKSEEDEM------VKELKMTVGEKMARKK 87
           +PSLR    KRF   C+  +  R+   +E  +  E          + L + + EK+ RKK
Sbjct: 37  SPSLRF---KRFS-VCYVVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKK 96

Query: 88  AERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM--------------------GVGMEFW 147
           +ER  YLIAA++SSFGITSMA +AVYYRFSWQM                     VGMEFW
Sbjct: 97  SERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFW 156

Query: 148 ARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLC 207
           ARWAH+ LWHASLW+MHESHH+PR G FE NDVFAI+NA PAI LLS+GFFN+G  PGLC
Sbjct: 157 ARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLC 216

Query: 208 FGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPI 257
           FGA                             GLGITVFG+AYMFVHDGLVH RFPVGPI
Sbjct: 217 FGA-----------------------------GLGITVFGIAYMFVHDGLVHKRFPVGPI 258

[Close]

BLAST of Lsi09G002270 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 232.6 bits (592), Expect = 5.0e-60
Identity = 128/234 (54.70%), Postives = 148/234 (63.25%), Query Frame = 0


Query: 43  CFAKQSSRRLLAMEKKKSEEDEMVKE--LKMTVGEKMARKKAERDAYLIAAIVSSFGITS 102
           CF  +  +     E  + + +  ++E      + EK+ARKK+ER  YL+AA++SSFGITS
Sbjct: 59  CFVLEDDKFKTQFEAGEEDIEMKIEEQISATRLAEKLARKKSERFTYLVAAVMSSFGITS 118

Query: 103 MAAIAVYYRFSWQM--------------------GVGMEFWARWAHKELWHASLWDMHES 162
           MA +AVYYRF WQM                     VGMEFWARWAHK LWHASLW MHES
Sbjct: 119 MAVMAVYYRFYWQMEGGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHES 178

Query: 163 HHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAF 222
           HH+PR G FE NDVFAIINAVPAIALL +GFF++G  PGLCFGA                
Sbjct: 179 HHKPREGPFELNDVFAIINAVPAIALLDYGFFHKGLIPGLCFGA---------------- 238

Query: 223 YDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAH 255
                        GLGITVFGMAYMFVHDGLVH RFPVGP+A VPYLR+VAAAH
Sbjct: 239 -------------GLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAH 263

[Close]

BLAST of Lsi09G002270 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 231.9 bits (590), Expect = 8.5e-60
Identity = 130/244 (53.28%), Postives = 149/244 (61.07%), Query Frame = 0


Query: 43  CFAKQSSRRLLAMEKKKSE----------EDEMVKELKMTVGEKMARKKAERDAYLIAAI 102
           CF  +  +   A   K+S+          E    K L + + EK ARKK+ER  YL+AA+
Sbjct: 58  CFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEEKSLAVRLAEKFARKKSERFTYLVAAV 117

Query: 103 VSSFGITSMAAIAVYYRFSWQM--------------------GVGMEFWARWAHKELWHA 162
           +SS GITSMA I+VYYRFSWQM                     +GME+WARWAH+ LWHA
Sbjct: 118 MSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAAIGMEYWARWAHRALWHA 177

Query: 163 SLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKS 222
           SLW MHESHHRPR G FE ND+FAIINAVPAIALLSFGF ++G  PGLCFGA        
Sbjct: 178 SLWHMHESHHRPREGPFELNDIFAIINAVPAIALLSFGFNHKGLIPGLCFGA-------- 237

Query: 223 KSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAA 257
                                GLGITVFGMAYMFVHDGLVH RFPVGPIA VPY +RVAA
Sbjct: 238 ---------------------GLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAA 272

[Close]

BLAST of Lsi09G002270 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.1e-58
Identity = 133/253 (52.57%), Postives = 155/253 (61.26%), Query Frame = 0


Query: 29  PSLRLGS--RKRFPWRCFAKQSSRRLLAM-EKKKSEEDEMVKELKMT--VGEKMARKKAE 88
           PSLR     R++    CF  +  ++   M +  K E      E+ MT  + +K  +KK+E
Sbjct: 33  PSLRFNGFRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSE 92

Query: 89  RDAYLIAAIVSSFGITSMAAIAVYYRFSWQM--------------------GVGMEFWAR 148
           R  YLIAA++SSFGITSMA +AVYYRFSWQM                     VGMEFWAR
Sbjct: 93  RFTYLIAAVMSSFGITSMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWAR 152

Query: 149 WAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLCFG 208
           WAH+ LWH SLW+MHESHH+PR GAFE NDVFAI NAVPAI LL +GF N+G  PGLCFG
Sbjct: 153 WAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFG 212

Query: 209 ANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPIAA 257
           A                             GLGIT+FGMAYMFVHDGLVH RFPVGPIA 
Sbjct: 213 A-----------------------------GLGITMFGMAYMFVHDGLVHKRFPVGPIAN 256

[Close]

BLAST of Lsi09G002270 vs. ExPASy TrEMBL
Match: A0A1S4DS92 (beta-carotene hydroxylase 2, chloroplastic-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 3.2e-94
Identity = 193/259 (74.52%), Postives = 205/259 (79.15%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLA--MEKK 60
           MATVISAVATA  Y++DY RLFQRPAGAPS      + FPWRCFAKQ  RRL+   M+KK
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPS------RGFPWRCFAKQRWRRLMVMKMKKK 60

Query: 61  KSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQ-MGVG 120
           KSEEDEMVK+LKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q M VG
Sbjct: 61  KSEEDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEVG 120

Query: 121 MEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFF 180
           MEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDVFAIINA+PAIALLSFG FN GFF
Sbjct: 121 MEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNHGFF 180

Query: 181 PGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFP 240
           PGLCFGA                             GLGITVFGMAYMFVHDGLVH RFP
Sbjct: 181 PGLCFGA-----------------------------GLGITVFGMAYMFVHDGLVHRRFP 224

Query: 241 VGPIAAVPYLRRVAAAHHV 257
           VGPIAAVPYLRRVAAAHH+
Sbjct: 241 VGPIAAVPYLRRVAAAHHI 224

[Close]

BLAST of Lsi09G002270 vs. ExPASy TrEMBL
Match: A0A1S3AUA9 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.1e-91
Identity = 192/279 (68.82%), Postives = 205/279 (73.48%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLA--MEKK 60
           MATVISAVATA  Y++DY RLFQRPAGAPS      + FPWRCFAKQ  RRL+   M+KK
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPS------RGFPWRCFAKQRWRRLMVMKMKKK 60

Query: 61  KSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM---- 120
           KSEEDEMVK+LKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q+    
Sbjct: 61  KSEEDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGG 120

Query: 121 -----------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAI 180
                             VGMEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDVFAI
Sbjct: 121 EFPILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAI 180

Query: 181 INAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGI 240
           INA+PAIALLSFG FN GFFPGLCFGA                             GLGI
Sbjct: 181 INAIPAIALLSFGLFNHGFFPGLCFGA-----------------------------GLGI 240

Query: 241 TVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHV 257
           TVFGMAYMFVHDGLVH RFPVGPIAAVPYLRRVAAAHH+
Sbjct: 241 TVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 244

[Close]

BLAST of Lsi09G002270 vs. ExPASy TrEMBL
Match: A0A1S4DT22 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.6e-91
Identity = 193/283 (68.20%), Postives = 206/283 (72.79%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLA--MEKK 60
           MATVISAVATA  Y++DY RLFQRPAGAPS      + FPWRCFAKQ  RRL+   M+KK
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPS------RGFPWRCFAKQRWRRLMVMKMKKK 60

Query: 61  KSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM---- 120
           KSEEDEMVK+LKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q+    
Sbjct: 61  KSEEDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGG 120

Query: 121 -----------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAI 180
                             VGMEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDVFAI
Sbjct: 121 EFPILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAI 180

Query: 181 INAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGI 240
           INA+PAIALLSFG FN GFFPGLCFGA                             GLGI
Sbjct: 181 INAIPAIALLSFGLFNHGFFPGLCFGA-----------------------------GLGI 240

Query: 241 TVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHVFIDV 261
           TVFGMAYMFVHDGLVH RFPVGPIAAVPYLRRVAAAHH   +V
Sbjct: 241 TVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHELEEV 248

[Close]

BLAST of Lsi09G002270 vs. ExPASy TrEMBL
Match: A0A0A0KNU9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152820 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 9.7e-91
Identity = 193/282 (68.44%), Postives = 205/282 (72.70%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRP-AGAPSLRLGSRKRFPWRCFAKQSSRR----LLAM 60
           M TVISAVATA PY++DY RLFQRP AGAPS        FPWRCFAKQ  RR    ++ M
Sbjct: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPS------PGFPWRCFAKQRWRRRRLMVVKM 60

Query: 61  EKKKSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM- 120
           +KKKSEEDEMVKELKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q+ 
Sbjct: 61  KKKKSEEDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIM 120

Query: 121 --------------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDV 180
                                VGMEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDV
Sbjct: 121 EGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDV 180

Query: 181 FAIINAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQG 240
           FAIINA+PAIALLSFG FNQGFFPGLCFGA                             G
Sbjct: 181 FAIINAIPAIALLSFGLFNQGFFPGLCFGA-----------------------------G 240

Query: 241 LGITVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHV 257
           LGITVFGMAYMFVHDGLVH RFPVGPIAAVPYLRRVAAAHH+
Sbjct: 241 LGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 247

[Close]

BLAST of Lsi09G002270 vs. ExPASy TrEMBL
Match: A0A6J1HZM0 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 2.2e-87
Identity = 182/257 (70.82%), Postives = 197/257 (76.65%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKS 60
           MAT ISAVATAAP    YRR+ QRPA AP+       R PWRCFAKQS+RRL  ++KK  
Sbjct: 1   MATGISAVATAAP----YRRIIQRPAKAPA----PANRVPWRCFAKQSTRRLSTVKKKCG 60

Query: 61  -EEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQMGVGME 120
             E EMV++LK+ V EKM RKKAERDAYL+AAIVSSFGITSMAAIAVYYRFSWQ+ VGME
Sbjct: 61  VSEVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQLEVGME 120

Query: 121 FWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPG 180
           FWARWAHK+LWH +LW+MH+SHHR R GAFE NDVFAIINAVPAIALLSFGFFNQGFFPG
Sbjct: 121 FWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIINAVPAIALLSFGFFNQGFFPG 180

Query: 181 LCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVG 240
           LCFGA                             GLGITVFGMAYMFVHDGLVH RFPVG
Sbjct: 181 LCFGA-----------------------------GLGITVFGMAYMFVHDGLVHRRFPVG 220

Query: 241 PIAAVPYLRRVAAAHHV 257
           PIAAVPYLRRVAAAHH+
Sbjct: 241 PIAAVPYLRRVAAAHHI 220

[Close]

BLAST of Lsi09G002270 vs. NCBI nr
Match: XP_038907153.1 (beta-carotene 3-hydroxylase, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-97
Identity = 199/283 (70.32%), Postives = 211/283 (74.56%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKS 60
           MATVI+AVATAAP+    +R F RPAGAP  RLG    FPWRCFA+Q SRRLLAM+KKKS
Sbjct: 1   MATVIAAVATAAPFN---QRFFHRPAGAPPPRLGFSNSFPWRCFAEQRSRRLLAMKKKKS 60

Query: 61  EEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM------ 120
           EEDEMVKELKM+V +KM RKKAERDAYL+AAIVSS G TSMAAIAVYYRFSWQM      
Sbjct: 61  EEDEMVKELKMSVDKKMGRKKAERDAYLVAAIVSSIGFTSMAAIAVYYRFSWQMEGREIP 120

Query: 121 --------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINA 180
                          VGMEFWARWAHKELWH SLWDMHESHHRPRVGAFE NDVFAIINA
Sbjct: 121 IWEMFGTFALSIGTAVGMEFWARWAHKELWHGSLWDMHESHHRPRVGAFELNDVFAIINA 180

Query: 181 VPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVF 240
           VPAI+LLSFGFFNQGFFPGLCFGA                             GLGITVF
Sbjct: 181 VPAISLLSFGFFNQGFFPGLCFGA-----------------------------GLGITVF 240

Query: 241 GMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHVFIDVSSV 264
           GMAYMFVHDGLVH RFP+GPIAAVPYLRRVAAAHHV ID+S +
Sbjct: 241 GMAYMFVHDGLVHRRFPLGPIAAVPYLRRVAAAHHVLIDMSFI 251

[Close]

BLAST of Lsi09G002270 vs. NCBI nr
Match: XP_038907152.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 360.9 bits (925), Expect = 9.3e-96
Identity = 195/276 (70.65%), Postives = 206/276 (74.64%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKS 60
           MATVI+AVATAAP+    +R F RPAGAP  RLG    FPWRCFA+Q SRRLLAM+KKKS
Sbjct: 1   MATVIAAVATAAPFN---QRFFHRPAGAPPPRLGFSNSFPWRCFAEQRSRRLLAMKKKKS 60

Query: 61  EEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM------ 120
           EEDEMVKELKM+V +KM RKKAERDAYL+AAIVSS G TSMAAIAVYYRFSWQM      
Sbjct: 61  EEDEMVKELKMSVDKKMGRKKAERDAYLVAAIVSSIGFTSMAAIAVYYRFSWQMEGREIP 120

Query: 121 --------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINA 180
                          VGMEFWARWAHKELWH SLWDMHESHHRPRVGAFE NDVFAIINA
Sbjct: 121 IWEMFGTFALSIGTAVGMEFWARWAHKELWHGSLWDMHESHHRPRVGAFELNDVFAIINA 180

Query: 181 VPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVF 240
           VPAI+LLSFGFFNQGFFPGLCFGA                             GLGITVF
Sbjct: 181 VPAISLLSFGFFNQGFFPGLCFGA-----------------------------GLGITVF 240

Query: 241 GMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHV 257
           GMAYMFVHDGLVH RFP+GPIAAVPYLRRVAAAHH+
Sbjct: 241 GMAYMFVHDGLVHRRFPLGPIAAVPYLRRVAAAHHI 244

[Close]

BLAST of Lsi09G002270 vs. NCBI nr
Match: XP_016898856.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X3 [Cucumis melo])

HSP 1 Score: 354.8 bits (909), Expect = 6.7e-94
Identity = 193/259 (74.52%), Postives = 205/259 (79.15%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLA--MEKK 60
           MATVISAVATA  Y++DY RLFQRPAGAPS      + FPWRCFAKQ  RRL+   M+KK
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPS------RGFPWRCFAKQRWRRLMVMKMKKK 60

Query: 61  KSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQ-MGVG 120
           KSEEDEMVK+LKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q M VG
Sbjct: 61  KSEEDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEVG 120

Query: 121 MEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFF 180
           MEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDVFAIINA+PAIALLSFG FN GFF
Sbjct: 121 MEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNHGFF 180

Query: 181 PGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFP 240
           PGLCFGA                             GLGITVFGMAYMFVHDGLVH RFP
Sbjct: 181 PGLCFGA-----------------------------GLGITVFGMAYMFVHDGLVHRRFP 224

Query: 241 VGPIAAVPYLRRVAAAHHV 257
           VGPIAAVPYLRRVAAAHH+
Sbjct: 241 VGPIAAVPYLRRVAAAHHI 224

[Close]

BLAST of Lsi09G002270 vs. NCBI nr
Match: XP_008437325.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 346.3 bits (887), Expect = 2.4e-91
Identity = 192/279 (68.82%), Postives = 205/279 (73.48%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLA--MEKK 60
           MATVISAVATA  Y++DY RLFQRPAGAPS      + FPWRCFAKQ  RRL+   M+KK
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPS------RGFPWRCFAKQRWRRLMVMKMKKK 60

Query: 61  KSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM---- 120
           KSEEDEMVK+LKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q+    
Sbjct: 61  KSEEDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGG 120

Query: 121 -----------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAI 180
                             VGMEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDVFAI
Sbjct: 121 EFPILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAI 180

Query: 181 INAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGI 240
           INA+PAIALLSFG FN GFFPGLCFGA                             GLGI
Sbjct: 181 INAIPAIALLSFGLFNHGFFPGLCFGA-----------------------------GLGI 240

Query: 241 TVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHV 257
           TVFGMAYMFVHDGLVH RFPVGPIAAVPYLRRVAAAHH+
Sbjct: 241 TVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 244

[Close]

BLAST of Lsi09G002270 vs. NCBI nr
Match: XP_016898855.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 345.1 bits (884), Expect = 5.3e-91
Identity = 193/283 (68.20%), Postives = 206/283 (72.79%), Query Frame = 0


Query: 1   MATVISAVATAAPYQFDYRRLFQRPAGAPSLRLGSRKRFPWRCFAKQSSRRLLA--MEKK 60
           MATVISAVATA  Y++DY RLFQRPAGAPS      + FPWRCFAKQ  RRL+   M+KK
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPAGAPS------RGFPWRCFAKQRWRRLMVMKMKKK 60

Query: 61  KSEEDEMVKELKMTVGEKMARKKAERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM---- 120
           KSEEDEMVK+LKM+V EKM +KKAER+ YLIAAIVSSFGITSMAAIAVYYRFS Q+    
Sbjct: 61  KSEEDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGG 120

Query: 121 -----------------GVGMEFWARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAI 180
                             VGMEFWARWAHKELWHASLWDMHESHH+PRVGAFE NDVFAI
Sbjct: 121 EFPILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAI 180

Query: 181 INAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGI 240
           INA+PAIALLSFG FN GFFPGLCFGA                             GLGI
Sbjct: 181 INAIPAIALLSFGLFNHGFFPGLCFGA-----------------------------GLGI 240

Query: 241 TVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHVFIDV 261
           TVFGMAYMFVHDGLVH RFPVGPIAAVPYLRRVAAAHH   +V
Sbjct: 241 TVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHELEEV 248

[Close]

BLAST of Lsi09G002270 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 233.4 bits (594), Expect = 2.1e-61
Identity = 135/255 (52.94%), Postives = 158/255 (61.96%), Query Frame = 0


Query: 28  APSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKSEEDEM------VKELKMTVGEKMARKK 87
           +PSLR    KRF   C+  +  R+   +E  +  E          + L + + EK+ RKK
Sbjct: 37  SPSLRF---KRFS-VCYVVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKK 96

Query: 88  AERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM--------------------GVGMEFW 147
           +ER  YLIAA++SSFGITSMA +AVYYRFSWQM                     VGMEFW
Sbjct: 97  SERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFW 156

Query: 148 ARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLC 207
           ARWAH+ LWHASLW+MHESHH+PR G FE NDVFAI+NA PAI LLS+GFFN+G  PGLC
Sbjct: 157 ARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLC 216

Query: 208 FGANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPI 257
           FGA                             GLGITVFG+AYMFVHDGLVH RFPVGPI
Sbjct: 217 FGA-----------------------------GLGITVFGIAYMFVHDGLVHKRFPVGPI 258

[Close]

BLAST of Lsi09G002270 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-59
Identity = 133/253 (52.57%), Postives = 155/253 (61.26%), Query Frame = 0


Query: 29  PSLRLGS--RKRFPWRCFAKQSSRRLLAM-EKKKSEEDEMVKELKMT--VGEKMARKKAE 88
           PSLR     R++    CF  +  ++   M +  K E      E+ MT  + +K  +KK+E
Sbjct: 33  PSLRFNGFRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSE 92

Query: 89  RDAYLIAAIVSSFGITSMAAIAVYYRFSWQM--------------------GVGMEFWAR 148
           R  YLIAA++SSFGITSMA +AVYYRFSWQM                     VGMEFWAR
Sbjct: 93  RFTYLIAAVMSSFGITSMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWAR 152

Query: 149 WAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLCFG 208
           WAH+ LWH SLW+MHESHH+PR GAFE NDVFAI NAVPAI LL +GF N+G  PGLCFG
Sbjct: 153 WAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFG 212

Query: 209 ANPSNPLKSKSLYDVAFYDWPLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPIAA 257
           A                             GLGIT+FGMAYMFVHDGLVH RFPVGPIA 
Sbjct: 213 A-----------------------------GLGITMFGMAYMFVHDGLVHKRFPVGPIAN 256

[Close]

BLAST of Lsi09G002270 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 174.5 bits (441), Expect = 1.1e-43
Identity = 98/183 (53.55%), Postives = 118/183 (64.48%), Query Frame = 0


Query: 28  APSLRLGSRKRFPWRCFAKQSSRRLLAMEKKKSEEDEM------VKELKMTVGEKMARKK 87
           +PSLR    KRF   C+  +  R+   +E  +  E          + L + + EK+ RKK
Sbjct: 37  SPSLRF---KRFS-VCYVVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKK 96

Query: 88  AERDAYLIAAIVSSFGITSMAAIAVYYRFSWQM--------------------GVGMEFW 147
           +ER  YLIAA++SSFGITSMA +AVYYRFSWQM                     VGMEFW
Sbjct: 97  SERSTYLIAAMLSSFGITSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFW 156

Query: 148 ARWAHKELWHASLWDMHESHHRPRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLC 185
           ARWAH+ LWHASLW+MHESHH+PR G FE NDVFAI+NA PAI LLS+GFFN+G  PGLC
Sbjct: 157 ARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLC 215

[Close]

BLAST of Lsi09G002270 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 104.0 bits (258), Expect = 1.9e-22
Identity = 87/233 (37.34%), Postives = 104/233 (44.64%), Query Frame = 0


Query: 29  PSLRLGS--RKRFPWRCFAKQSSRRLLAM-EKKKSEEDEMVKELKMT--VGEKMARKKAE 88
           PSLR     R++    CF  +  ++   M +  K E      E+ MT  + +K  +KK+E
Sbjct: 33  PSLRFNGFRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSE 92

Query: 89  RDAYLIAAIVSSFGITSMAAIAVYYRFSWQMGVGMEFWARWAHKELWHASLWDMHESHHR 148
           R  YLIAA++SSFGITSMA +AVYYRFSWQM  G                          
Sbjct: 93  RFTYLIAAVMSSFGITSMAIMAVYYRFSWQMKGG-------------------------- 152

Query: 149 PRVGAFEFNDVFAIINAVPAIALLSFGFFNQGFFPGLCFGANPSNPLKSKSLYDVAFYDW 208
             V   E    FA          LS G                                 
Sbjct: 153 -EVSVLEMFGTFA----------LSVG--------------------------------- 187

Query: 209 PLTVITNFQQGLGITVFGMAYMFVHDGLVHCRFPVGPIAAVPYLRRVAAAHHV 257
                     GLGIT+FGMAYMFVHDGLVH RFPVGPIA VPYLR+VAAAH +
Sbjct: 213 --------AAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQL 187

The following BLAST results are available for this feature:

BLAST of Lsi09G002270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B3SGL0	1.3e-60	57.92	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	2.9e-60	52.94	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49814	5.0e-60	54.70	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
O49815	8.5e-60	53.28	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	2.1e-58	52.57	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi09G002270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DS92	3.2e-94	74.52	beta-carotene hydroxylase 2, chloroplastic-like isoform X3 OS=Cucumis melo OX=36...	[more]
A0A1S3AUA9	1.1e-91	68.82	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=36...	[more]
A0A1S4DT22	2.6e-91	68.20	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=36...	[more]
A0A0A0KNU9	9.7e-91	68.44	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1HZM0	2.2e-87	70.82	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X2 OS=Cucurbita maxima...	[more]

BLAST of Lsi09G002270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907153.1	1.3e-97	70.32	beta-carotene 3-hydroxylase, chloroplastic-like isoform X2 [Benincasa hispida]	[more]
XP_038907152.1	9.3e-96	70.65	beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Benincasa hispida]	[more]
XP_016898856.1	6.7e-94	74.52	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X3 [Cucumis m...	[more]
XP_008437325.1	2.4e-91	68.82	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucumis m...	[more]
XP_016898855.1	5.3e-91	68.20	PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucumis m...	[more]

BLAST of Lsi09G002270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	2.1e-61	52.94	beta-hydroxylase 1	[more]
AT5G52570.1	1.5e-59	52.57	beta-carotene hydroxylase 2	[more]
AT4G25700.2	1.1e-43	53.55	beta-hydroxylase 1	[more]
AT5G52570.2	1.9e-22	37.34	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 42..114 coord: 116..256

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G002270.1	Lsi09G002270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity