Lsi09G002070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G002070
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr09: 2157395 .. 2159096 (-)
RNA-Seq ExpressionLsi09G002070
SyntenyLsi09G002070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGGCAGAAGGCCTCAACCATAGGTCGTTAAAAGAACTAGCAGACGAGGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATACGCAACCCATATGTTTACTCGCTATGCAAATAGGACGATTGAAGAAAACTTTGATCTTCTTGATTTAAAACAAGCAAAAGTTGGATTGGCAAAATATAATCCTTTTGTGTTGAACAAGACTATTGCTGAAGCGTATGAAGCTGTTGTTGATGTGTTGGGAGCAAGTGGTCTAATGGTCATCGCTGACAATCACATTAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGCTTCTTCGGAAACCGCAATTTTGACCCTCAAGAATGGCTACAAGGTCTTAACTTAGTTGCTCAACGCTTTCGCAACAAATCCACGGTATGTAACATCAAGAGATTTTCTTTAATTCCTAAATCTAAATTTAAGCTTTTAAAAAGTACTTCTATTACATTTCATTAACAAAGAACATTTTTTAATGGTTGTTTAGGTGGTAGGAATGAGTTTACGAAATGAGATTCGAGGCTTTATGGAAAATGCAAATGATTGGAACAACTATGTAACTCAAGGAGTAACCACGATTCATAACATAAATTCGGAAGTTTTAGTGATTGTTTCAGGCTTAAATTATGACAATGATCTCCGATGCTTAAAGGAAAAGCCTTTGGCTGTAAACACCTTAGACAATAAGTTGGTTTTCGAGGTACACTTGTATTCTTTCAGTGGAGATTCCGAGAGCAAGTTTGTACAACAACCATTAAACAATATTTGTGCAAATATTATGAATGGATTTATAGACCATGCTGGGTTTGTAATGGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGATTCATGAGTTGCTTCACAGCCCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACTTGGCAAGGTAGCTATTATTACAGAGAAGGTCAAGCAGAGCCTGCAGAAGCTTTTGGAGTGCTCGACTCTAGTTGGACTCAAATTAAAAATCCTAACTTTGTAAAGAAGTTTCAACTATTGCAGACCATGTTGCAAGGTAACTATATTAATGATTACGAGTATATGCAAGGATTTATAACTTGTAAACTCCTGTGATTTGTGAAAATAAATGTTATAGTTCAACTAGAATTTTGGAAAACTAGTTAAGTAACAATTTGGATAACATCTTTTTTATGGTATACAGATCCAAATTCCAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTGGCCAATGCATCCAAGTCTCTAATGACAACAAAGAAATTTTTCTCACAAATTGCTCCACCTCAAGTCGATGGAGTCATGACAACGATGGGACTCCTATTAGGATGTCAAACACCGGTTTGTGCTTGAAAGCTAGCGGGGAAGGCCTTGAGGCATCTCTTTCAACTGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCCATTTCAAACTCTAAGCTTCATTTGGCCACCTTCACTCAAGATGGAAAGAGTCTTTGTTTGCAAATTGAAAGCTCCAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACGAATGGCGATCCAAAATGCCTTCAAGACACACAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTGTGA

mRNA sequence

ATGCTGGCAGAAGGCCTCAACCATAGGTCGTTAAAAGAACTAGCAGACGAGGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATACGCAACCCATATGTTTACTCGCTATGCAAATAGGACGATTGAAGAAAACTTTGATCTTCTTGATTTAAAACAAGCAAAAGTTGGATTGGCAAAATATAATCCTTTTGTGTTGAACAAGACTATTGCTGAAGCGTATGAAGCTGTTGTTGATGTGTTGGGAGCAAGTGGTCTAATGGTCATCGCTGACAATCACATTAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGCTTCTTCGGAAACCGCAATTTTGACCCTCAAGAATGGCTACAAGGTCTTAACTTAGTTGCTCAACGCTTTCGCAACAAATCCACGGTGGTAGGAATGAGTTTACGAAATGAGATTCGAGGCTTTATGGAAAATGCAAATGATTGGAACAACTATGTAACTCAAGGAGTAACCACGATTCATAACATAAATTCGGAAGTTTTAGTGATTGTTTCAGGCTTAAATTATGACAATGATCTCCGATGCTTAAAGGAAAAGCCTTTGGCTGTAAACACCTTAGACAATAAGTTGGTTTTCGAGGTACACTTGTATTCTTTCAGTGGAGATTCCGAGAGCAAGTTTGTACAACAACCATTAAACAATATTTGTGCAAATATTATGAATGGATTTATAGACCATGCTGGGTTTGTAATGGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGATTCATGAGTTGCTTCACAGCCCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACTTGGCAAGGTAGCTATTATTACAGAGAAGGTCAAGCAGAGCCTGCAGAAGCTTTTGGAGTGCTCGACTCTAGTTGGACTCAAATTAAAAATCCTAACTTTGTAAAGAAGTTTCAACTATTGCAGACCATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTGGCCAATGCATCCAAGTCTCTAATGACAACAAAGAAATTTTTCTCACAAATTGCTCCACCTCAAGTCGATGGAGTCATGACAACGATGGGACTCCTATTAGGATGTCAAACACCGGTTTGTGCTTGAAAGCTAGCGGGGAAGGCCTTGAGGCATCTCTTTCAACTGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCCATTTCAAACTCTAAGCTTCATTTGGCCACCTTCACTCAAGATGGAAAGAGTCTTTGTTTGCAAATTGAAAGCTCCAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACGAATGGCGATCCAAAATGCCTTCAAGACACACAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTGTGA

Coding sequence (CDS)

ATGCTGGCAGAAGGCCTCAACCATAGGTCGTTAAAAGAACTAGCAGACGAGGCAATCAAGTTAAGATTCAATTGTGTACGACTCACATACGCAACCCATATGTTTACTCGCTATGCAAATAGGACGATTGAAGAAAACTTTGATCTTCTTGATTTAAAACAAGCAAAAGTTGGATTGGCAAAATATAATCCTTTTGTGTTGAACAAGACTATTGCTGAAGCGTATGAAGCTGTTGTTGATGTGTTGGGAGCAAGTGGTCTAATGGTCATCGCTGACAATCACATTAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGCAATGGCTTCTTCGGAAACCGCAATTTTGACCCTCAAGAATGGCTACAAGGTCTTAACTTAGTTGCTCAACGCTTTCGCAACAAATCCACGGTGGTAGGAATGAGTTTACGAAATGAGATTCGAGGCTTTATGGAAAATGCAAATGATTGGAACAACTATGTAACTCAAGGAGTAACCACGATTCATAACATAAATTCGGAAGTTTTAGTGATTGTTTCAGGCTTAAATTATGACAATGATCTCCGATGCTTAAAGGAAAAGCCTTTGGCTGTAAACACCTTAGACAATAAGTTGGTTTTCGAGGTACACTTGTATTCTTTCAGTGGAGATTCCGAGAGCAAGTTTGTACAACAACCATTAAACAATATTTGTGCAAATATTATGAATGGATTTATAGACCATGCTGGGTTTGTAATGGAAGGATCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCAGAAAATCGATTCATGAGTTGCTTCACAGCCCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACTTGGCAAGGTAGCTATTATTACAGAGAAGGTCAAGCAGAGCCTGCAGAAGCTTTTGGAGTGCTCGACTCTAGTTGGACTCAAATTAAAAATCCTAACTTTGTAAAGAAGTTTCAACTATTGCAGACCATGTTGCAAGATCCAAATTCCAATGCATCGTTCTCATATGTTATATATCATCCACAAAGTGGCCAATGCATCCAAGTCTCTAATGACAACAAAGAAATTTTTCTCACAAATTGCTCCACCTCAAGTCGATGGAGTCATGACAACGATGGGACTCCTATTAGGATGTCAAACACCGGTTTGTGCTTGAAAGCTAGCGGGGAAGGCCTTGAGGCATCTCTTTCAACTGACTGCTTAGGCAAACAAAGTGTTTGGAGTGCCATTTCAAACTCTAAGCTTCATTTGGCCACCTTCACTCAAGATGGAAAGAGTCTTTGTTTGCAAATTGAAAGCTCCAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACGAATGGCGATCCAAAATGCCTTCAAGACACACAAAGCCAATGGTTTGAACTTGTTGGAACCAACACATTGTGA

Protein sequence

MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLAKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEWLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVSGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSAISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNTL
Homology
BLAST of Lsi09G002070 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.3e-98
Identity = 196/493 (39.76%), Postives = 289/493 (58.62%), Query Frame = 0

Query: 2   LAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTR--YANRTIEENFDLLDLKQAKVGL 61
           L EGLN   +  +A     L FNCVRLTY+ HM TR  Y N T+ + F  L+L +A  G+
Sbjct: 62  LPEGLNRLPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGI 121

Query: 62  AKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQ 121
              NP +L+     AY  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+R F+P 
Sbjct: 122 EHNNPELLDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPN 181

Query: 122 EWLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIV 181
            W++GL L+A  F N   VV MSLRNE+RG       W+ ++  G  T+H  N +VLVI+
Sbjct: 182 TWVEGLGLMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVIL 241

Query: 182 SGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFI 241
           SGL +D DL  L   P+ +     K+V+E H YSF     +       N++C N    F 
Sbjct: 242 SGLQFDTDLSFLPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFK 301

Query: 242 DHAGFVMEGSN--PFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSY 301
            + GFV   +N    PLF+SE+G DQR V+D +NR+++C  A+LA++DLDWALWT  GSY
Sbjct: 302 SNVGFVTSSANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSY 361

Query: 302 YYREGQ---AEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPN-SNASFSYVIYHPQ 361
           YYR  +    +  E +G  +  W++I+NP+F+ + + +Q  +QDP  +   +  +IYHP 
Sbjct: 362 YYRSDKQPVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPA 421

Query: 362 SGQCIQVSNDNKEIFLTNC-STSSRWSHD-NDGTPIRMSNTGLCLKASGEGLEASLSTDC 421
           SG C++ S     + L +C S  SRW++D +   PI +  +  C+   G GL A ++ +C
Sbjct: 422 SGLCVE-SGIGNTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENC 481

Query: 422 LG-KQSVWSAISNSKLHLAT--FTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQ-- 480
                ++WS +S+++L L T    +DGK   + ++ S S  I TN CIC   D  C    
Sbjct: 482 SAPNNTLWSTVSSAQLQLGTRVLGKDGKEKWMCLDGSKSPLISTNECICIT-DSHCYPKL 541

BLAST of Lsi09G002070 vs. ExPASy Swiss-Prot
Match: P54583 (Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=Acel_0614 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 5.4e-12
Identity = 75/336 (22.32%), Postives = 134/336 (39.88%), Query Frame = 0

Query: 2   LAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLAK 61
           +  GL  R  + + D+   L +N +RL Y+  +       T+  + +   + Q   GL  
Sbjct: 78  VVHGLWSRDYRSMLDQIKSLGYNTIRLPYSDDIL---KPGTMPNSINFYQMNQDLQGLTS 137

Query: 62  YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEW 121
               V++K +A A        G  GL +I D H    R  CS    +  +   +     W
Sbjct: 138 LQ--VMDKIVAYA--------GQIGLRIILDRH----RPDCS--GQSALWYTSSVSEATW 197

Query: 122 LQGLNLVAQRFRNKSTVVGMSLRNEIR-----GFMENANDWNNYVTQGVTTIHNINSEVL 181
           +  L  +AQR++   TVVG  L NE       G  + + DW     +    + ++N  +L
Sbjct: 198 ISDLQALAQRYKGNPTVVGFDLHNEPHDPACWGCGDPSIDWRLAAERAGNAVLSVNPNLL 257

Query: 182 VIVSGL-NYDND-------LRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLN 241
           + V G+ +Y+ D       L+   + P+ +N + N+LV+  H Y+ S   ++ F      
Sbjct: 258 IFVEGVQSYNGDSYWWGGNLQGAGQYPVVLN-VPNRLVYSAHDYATSVYPQTWFSDPTFP 317

Query: 242 NICANIMNGFIDHAGFVMEGSNPFPLFVSEYGYD-QREVDDAENRFMSCF---TAHLAQK 301
           N    I N    + G++    N  P+++ E+G   Q   D    + +  +   TA     
Sbjct: 318 NNMPGIWN---KNWGYLF-NQNIAPVWLGEFGTTLQSTTDQTWLKTLVQYLRPTAQYGAD 377

Query: 302 DLDWALWTWQGSYYYREGQAEPAEAFGVLDSSWTQI 321
              W  W+W           +  +  G+L   W  +
Sbjct: 378 SFQWTFWSW---------NPDSGDTGGILKDDWQTV 380

BLAST of Lsi09G002070 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 7.8e-11
Identity = 83/353 (23.51%), Postives = 136/353 (38.53%), Query Frame = 0

Query: 5   GLNHRSLKELADEAIKLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKQAKVGLAK 64
           GL  R+ K++  +   L FN VRL +        T  A+     N DL  L   +     
Sbjct: 61  GLWARNWKDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ----- 120

Query: 65  YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQEW 124
               +L+K IAE          A G+ V+ D+H      C  + +    +   ++   +W
Sbjct: 121 ----ILDKVIAE--------FNARGMYVLLDHHTPD---CAGISE---LWYTGSYTEAQW 180

Query: 125 LQGLNLVAQRFRNKSTVVGMSLRNEIRGFM-----ENANDWNNYVTQGVTTIHNINSEVL 184
           L  L  VA R++N   V+G+ L+NE  G         A DWN    +G   +  +  + L
Sbjct: 181 LADLRFVANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWL 240

Query: 185 VIVSGLNYDNDLRCLKE--------KPLAVNTLD---NKLVFEVHLYSFSGDSESKFVQQ 244
           + V G+  DN +             +PLA   L+   N+L+   H+Y         FVQ 
Sbjct: 241 IAVEGIT-DNPVCSTNGGIFWGGNLQPLACTPLNIPANRLLLAPHVY-----GPDVFVQS 300

Query: 245 PLN--NICANIMNGFIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQ 304
             N  N   N+   +  H G   + +    L + E+G    E D  +  +      +L  
Sbjct: 301 YFNDSNFPNNMPAIWERHFG---QFAGTHALLLGEFGGKYGEGDARDKTWQDALVKYLRS 360

Query: 305 KDLDWAL-WTWQGSYYYREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTM 336
           K ++    W+W              +  G+L   WT ++      K  LL+T+
Sbjct: 361 KGINQGFYWSW---------NPNSGDTGGILRDDWTSVRQ----DKMTLLRTL 368

BLAST of Lsi09G002070 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 1.5e-09
Identity = 66/339 (19.47%), Postives = 135/339 (39.82%), Query Frame = 0

Query: 5   GLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLAKYNP 64
           GL  RS+ ++ D+  K  +N +RL Y+  +F   +        D +D  +        NP
Sbjct: 73  GLWSRSMDDMLDQVKKEGYNLIRLPYSNQLFDSSSRP------DSIDYHK--------NP 132

Query: 65  FVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNG----FFGNRNFDPQE 124
            ++     +  + +++  G  G+ +I D H            G+G     +    +    
Sbjct: 133 DLVGLNPIQIMDKLIEKAGQRGIQIILDRH----------RPGSGGQSELWYTSQYPESR 192

Query: 125 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFME----NAN-DWNNYVTQGVTTIHNINSEV 184
           W+    ++A R++N  TV+G  L NE  G       NA+ DW     +    I ++N   
Sbjct: 193 WISDWKMLADRYKNNPTVIGADLHNEPHGQASWGTGNASTDWRLAAQRAGNAILSVNPNW 252

Query: 185 LVIVSGLNYD-----------NDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQ 244
           L++V G++++            +L  +   P+ ++ + N++V+  H Y   G S   +  
Sbjct: 253 LILVEGVDHNVQGNNSQYWWGGNLTGVANYPVVLD-VPNRVVYSPHDYG-PGVSSQPWFN 312

Query: 245 QPLNNICANIMNGFIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQK 304
            P     +N+   +    G++ +  N  P+ V E+G    ++   E ++ +    ++   
Sbjct: 313 DPA--FPSNLPAIWDQTWGYISK-QNIAPVLVGEFGGRNVDLSCPEGKWQNALVHYIGAN 372

Query: 305 DLDWALWTWQGSYYYREGQAEPAEAFGVLDSSWTQIKNP 324
           +L +  W+               +  G+L   WT    P
Sbjct: 373 NLYFTYWSL---------NPNSGDTGGLLLDDWTTWNRP 373

BLAST of Lsi09G002070 vs. ExPASy TrEMBL
Match: A0A5A7TH83 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002080 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 2.7e-264
Identity = 437/481 (90.85%), Postives = 460/481 (95.63%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 1   MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVT IHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTAIHNINSEVLVIVS 180

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFID 240

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSEYGYDQREV+DAENRFMSCFTAHL  +DLDWALW WQGSYY+R
Sbjct: 241 HAGFVMQGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLVLRDLDWALWAWQGSYYFR 300

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDP SN SFSY+IYHPQSGQCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPISNTSFSYIIYHPQSGQCIQV 360

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLTNCST +RWSH+NDGTPI+MS+TGLCLKASGEGLE SLSTDCL +QSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIKMSSTGLCLKASGEGLETSLSTDCLSQQSVWSA 420

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLATFTQ GKSLCLQI+ S+SSK+VTNSCICTNGDP CLQDT+SQWFELVGTNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQIDGSSSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Lsi09G002070 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.5e-264
Identity = 439/481 (91.27%), Postives = 461/481 (95.84%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 56  MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 115

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 116 QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 175

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 176 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 235

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFID
Sbjct: 236 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFID 295

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+R
Sbjct: 296 HAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFR 355

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDPNSN SFSYVIYHPQS QCIQV
Sbjct: 356 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQV 415

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLT+CST +RWSH ND TPI+MS+TGLCLKASGEGLEASLSTDCL +QSVWSA
Sbjct: 416 SNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSA 475

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLAT TQ GKSLCLQI+SSNSSK+VTNSCICTNGDP CLQDT+SQWFELVGTNT
Sbjct: 476 ISNSKLHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 535

Query: 481 L 482
           L
Sbjct: 536 L 536

BLAST of Lsi09G002070 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.5e-262
Identity = 436/481 (90.64%), Postives = 459/481 (95.43%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 56  MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 115

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 116 QYNPFVLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 175

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 176 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 235

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFID
Sbjct: 236 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 295

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+R
Sbjct: 296 HAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFR 355

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDPNSNASFSYVIYHPQ+ QCIQV
Sbjct: 356 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQV 415

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLT+CST +RWSH ND TPI+MS+TGLCLKASGEGLEASLSTDCL +QSVWSA
Sbjct: 416 SNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSA 475

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLAT  Q G SLCLQI+SSNSSK+VTNSCICTNGDP CLQDT+SQWFELV TNT
Sbjct: 476 ISNSKLHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNT 535

Query: 481 L 482
           L
Sbjct: 536 L 536

BLAST of Lsi09G002070 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 1.9e-262
Identity = 438/482 (90.87%), Postives = 460/482 (95.44%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 59  MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 118

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
            YNPFVLNKTIAEAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 119 LYNPFVLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 178

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 179 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 238

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFID
Sbjct: 239 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFID 298

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+R
Sbjct: 299 HAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFR 358

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDPNSNASFSYVIYHPQS QCIQV
Sbjct: 359 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQV 418

Query: 361 SNDNKEIFLTNC-STSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWS 420
           SNDNKEIFLT+C ST +RWSH ND TPI+MS+TGLCLKASGEGLEASLSTDCL +QSVWS
Sbjct: 419 SNDNKEIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWS 478

Query: 421 AISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTN 480
           AISNSKLHLAT TQ GKSLCLQI+SSN SK+VTNSCICTNGDP CLQDT+SQWFELVGTN
Sbjct: 479 AISNSKLHLATITQGGKSLCLQIDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTN 538

Query: 481 TL 482
           TL
Sbjct: 539 TL 540

BLAST of Lsi09G002070 vs. ExPASy TrEMBL
Match: A0A5D3D4P4 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5066G00050 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 8.0e-261
Identity = 439/481 (91.27%), Postives = 455/481 (94.59%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LKELADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL QAK GL 
Sbjct: 1   MLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLGQAKAGLT 60

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGNR FDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQE 120

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRF NKSTVVGMSLRNEIRG MENANDWN+YVTQGVTTIHNIN EVLVIV 
Sbjct: 121 WLQGLSLVAQRFNNKSTVVGMSLRNEIRGMMENANDWNHYVTQGVTTIHNINPEVLVIVG 180

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V+TLDNKLVFEVHLYSFSG SESKFVQQPLNNICA I+N FID
Sbjct: 181 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGASESKFVQQPLNNICAKIINEFID 240

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HA FV+EGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR
Sbjct: 241 HAEFVIEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAE  E FGVL+S+WTQIKNPNFV+KFQLLQTMLQDPNSNASFSYVIYHPQSGQCI+V
Sbjct: 301 EGQAELPETFGVLESNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIEV 360

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNK+IFLTNCSTSSRWSHDND TPI+MSNTGLCLKASGEGL ASLS DCLGKQSVWSA
Sbjct: 361 SNDNKDIFLTNCSTSSRWSHDNDSTPIKMSNTGLCLKASGEGLAASLSNDCLGKQSVWSA 420

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLAT T++GKSLCLQIESSNSSKIVTNSCICT  DP CLQDTQSQWFELV TNT
Sbjct: 421 ISNSKLHLATVTENGKSLCLQIESSNSSKIVTNSCICTTDDPTCLQDTQSQWFELVETNT 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Lsi09G002070 vs. NCBI nr
Match: KAE8648504.1 (hypothetical protein Csa_008732 [Cucumis sativus])

HSP 1 Score: 921.0 bits (2379), Expect = 4.2e-264
Identity = 441/481 (91.68%), Postives = 462/481 (96.05%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           MLAEGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 1   MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 180

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 240

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+G NPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQ+DLDWALW WQGSYY+R
Sbjct: 241 HAGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQRDLDWALWAWQGSYYFR 300

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQIKNPNFV+KFQLLQTML DPNSNASFSYVIYHPQS QCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQIKNPNFVQKFQLLQTMLHDPNSNASFSYVIYHPQSSQCIQV 360

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLTNCST +RWSH+NDGTPI MS+TGL LKASGEGLEASLSTD L +QSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGEGLEASLSTDTLSQQSVWSA 420

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLATFTQ GKSLCLQ++SSNSSK+VTNSCICTNGDP CLQDT+SQWFELV TNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQMDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVETNT 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Lsi09G002070 vs. NCBI nr
Match: KAA0042710.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 920.6 bits (2378), Expect = 5.5e-264
Identity = 437/481 (90.85%), Postives = 460/481 (95.63%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 1   MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 61  QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVT IHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTAIHNINSEVLVIVS 180

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFID 240

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSEYGYDQREV+DAENRFMSCFTAHL  +DLDWALW WQGSYY+R
Sbjct: 241 HAGFVMQGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLVLRDLDWALWAWQGSYYFR 300

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDP SN SFSY+IYHPQSGQCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPISNTSFSYIIYHPQSGQCIQV 360

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLTNCST +RWSH+NDGTPI+MS+TGLCLKASGEGLE SLSTDCL +QSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIKMSSTGLCLKASGEGLETSLSTDCLSQQSVWSA 420

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLATFTQ GKSLCLQI+ S+SSK+VTNSCICTNGDP CLQDT+SQWFELVGTNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQIDGSSSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Lsi09G002070 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 920.2 bits (2377), Expect = 7.2e-264
Identity = 439/481 (91.27%), Postives = 461/481 (95.84%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 56  MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 115

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 116 QYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 175

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 176 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 235

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLN+ICANIMNGFID
Sbjct: 236 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFID 295

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+R
Sbjct: 296 HAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFR 355

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDPNSN SFSYVIYHPQS QCIQV
Sbjct: 356 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQV 415

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLT+CST +RWSH ND TPI+MS+TGLCLKASGEGLEASLSTDCL +QSVWSA
Sbjct: 416 SNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSA 475

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLAT TQ GKSLCLQI+SSNSSK+VTNSCICTNGDP CLQDT+SQWFELVGTNT
Sbjct: 476 ISNSKLHLATVTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 535

Query: 481 L 482
           L
Sbjct: 536 L 536

BLAST of Lsi09G002070 vs. NCBI nr
Match: KAE8650477.1 (hypothetical protein Csa_010217 [Cucumis sativus])

HSP 1 Score: 919.1 bits (2374), Expect = 1.6e-263
Identity = 438/481 (91.06%), Postives = 462/481 (96.05%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           MLAEGLNHR LK+LADEAIKLRFNCVRLTYATHM TRYANRTIEENFDLLDLKQAK GLA
Sbjct: 1   MLAEGLNHRPLKDLADEAIKLRFNCVRLTYATHMLTRYANRTIEENFDLLDLKQAKAGLA 60

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKT+AEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 61  QYNPFVLNKTVAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNNNFDPQE 120

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 121 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 180

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V+TLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFID
Sbjct: 181 GLNYDNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 240

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           H GFVM+G NPFPLFVSEYGYDQREV+DAENRFMSCFTAHL Q+DLDWALW WQGSYY+R
Sbjct: 241 HVGFVMQGPNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLTQRDLDWALWAWQGSYYFR 300

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQIKNPNFV+KFQLLQTMLQDPNSNASFSYVIYHPQS QCIQV
Sbjct: 301 EGQAEPGESFGVLDSNWTQIKNPNFVRKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQV 360

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLTNCST +RWSH+NDGTPI MS+TGL LKASG+GLEASLS+D L +QSVWSA
Sbjct: 361 SNDNKEIFLTNCSTPTRWSHNNDGTPIEMSSTGLYLKASGKGLEASLSSDTLSQQSVWSA 420

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLATFTQ GKSLCLQI+SSNSSK+VTNSCICTNGDP CLQDT+SQWFELVGTNT
Sbjct: 421 ISNSKLHLATFTQGGKSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 480

Query: 481 L 482
           L
Sbjct: 481 L 481

BLAST of Lsi09G002070 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 914.8 bits (2363), Expect = 3.0e-262
Identity = 436/481 (90.64%), Postives = 459/481 (95.43%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKVGLA 60
           ML EGLNHR LK+LADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAK GLA
Sbjct: 56  MLIEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLA 115

Query: 61  KYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDPQE 120
           +YNPFVLNKTIAEAYEAVVD+LGASGLMVIADNHISQPRWCCSLDDGNGFFGN NFDPQE
Sbjct: 116 QYNPFVLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQE 175

Query: 121 WLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVIVS 180
           WLQGL+LVAQRFRNKSTVVGMSLRNEIRGFMENANDWN Y+TQGVTTIHNINSEVLVIVS
Sbjct: 176 WLQGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVS 235

Query: 181 GLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGFID 240
           GLNYDNDLRCLKEKPL V TLDNKLVFEVHLYSFSGDSESKFV+QPLNNICANIMNGFID
Sbjct: 236 GLNYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFID 295

Query: 241 HAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYR 300
           HAGFVM+GSNPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+R
Sbjct: 296 HAGFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFR 355

Query: 301 EGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQV 360
           EGQAEP E+FGVLDS+WTQ+KNPNF KKFQLLQTMLQDPNSNASFSYVIYHPQ+ QCIQV
Sbjct: 356 EGQAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQV 415

Query: 361 SNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCLGKQSVWSA 420
           SNDNKEIFLT+CST +RWSH ND TPI+MS+TGLCLKASGEGLEASLSTDCL +QSVWSA
Sbjct: 416 SNDNKEIFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSA 475

Query: 421 ISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFELVGTNT 480
           ISNSKLHLAT  Q G SLCLQI+SSNSSK+VTNSCICTNGDP CLQDT+SQWFELV TNT
Sbjct: 476 ISNSKLHLATVAQGGNSLCLQIDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNT 535

Query: 481 L 482
           L
Sbjct: 536 L 536

BLAST of Lsi09G002070 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.0e-102
Identity = 189/481 (39.29%), Postives = 296/481 (61.54%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYA---NRTIEENFDLLDLKQAKV 60
           ++AEGL+ + +  +A + +++ FNCVRLT+   + T      N T+ ++F  L L    V
Sbjct: 64  VVAEGLSKQPVDAVAKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIV 123

Query: 61  GLAKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFD 120
           G    NP +++  + EAY+ VV  LG + +MVI DNH+++P WCC+ DDGNGFFG++ FD
Sbjct: 124 GFQTNNPSIIDLPLIEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFD 183

Query: 121 PQEWLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLV 180
           P  W+  L  +A  F   S VVGMSLRNE+RG  +N NDW  Y+ QG   +H+ N++VLV
Sbjct: 184 PTVWVAALKKMAATFNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLV 243

Query: 181 IVSGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNG 240
           I+SGL++D DL  ++ +P+ + +   KLVFE+H YSFS D  S     P N+IC  ++N 
Sbjct: 244 ILSGLSFDADLSFVRSRPVKL-SFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNR 303

Query: 241 FIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSY 300
             +  G+++  +  FPLF+SE+G D+R V+  +NR+  C T   A+ D+DW+LW   GSY
Sbjct: 304 IGNGGGYLL--NQGFPLFLSEFGIDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSY 363

Query: 301 YYREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQC 360
           Y R+G+    E +GVLDS W  ++N +F++K   LQ+ LQ P        +++HP +G C
Sbjct: 364 YLRQGKVGMNEYYGVLDSDWISVRNSSFLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLC 423

Query: 361 IQVS-NDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLS-TDCLGKQ 420
           I  S +D K + L  C++S  WS+      +R+ +  LCL+++G     +++ T C    
Sbjct: 424 IVRSLDDPKMLTLGPCNSSEPWSYTKKA--LRIKDQQLCLQSNGPKNPVTMTRTSCSTSG 483

Query: 421 SVWSAISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFEL 477
           S W  IS S++HLA+ T +  SLCL ++++N+  +V N+C C + D  C  +  SQWF++
Sbjct: 484 SKWQTISASRMHLASTTSNKTSLCLDVDTANN--VVANACKCLSKDKSC--EPMSQWFKI 533

BLAST of Lsi09G002070 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 339.0 bits (868), Expect = 6.4e-93
Identity = 192/485 (39.59%), Postives = 279/485 (57.53%), Query Frame = 0

Query: 2   LAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKQAKVG 61
           +AEGL+ + L  +A++ + + FNCVRLT+  ++ T     A  T+ ++     L +A  G
Sbjct: 55  VAEGLSKQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSG 114

Query: 62  LAKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDP 121
              +NP +L+  + +A++ VV  L    +MVI DNHISQP WCCS +DGNGFFG+++ +P
Sbjct: 115 FQTHNPTILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNP 174

Query: 122 QEWLQGLNLVAQRFRN-KSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLV 181
           Q W++GL  +A  F N  S VVGMSLRNE+RG  +N  DW  Y+ +G   +H++N  VLV
Sbjct: 175 QVWIKGLKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLV 234

Query: 182 IVSGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNG 241
           IVSGLNY  DL  L+E+P  V +   K+VFE+H Y F    E       LN IC      
Sbjct: 235 IVSGLNYATDLSFLRERPFEV-SFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEK 294

Query: 242 FIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSY 301
            +  +GF++E     PLFVSE+G DQR  +  +N+F+SCF A  A +DLDW+LWT  GSY
Sbjct: 295 MMKMSGFLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSY 354

Query: 302 YYREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQT-----MLQDPNSNASFSYVIYHP 361
           Y RE      E++GVLD +W+ I+N   ++    +QT     M   P        +++HP
Sbjct: 355 YIREKSIGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGLMETQPKK------IMFHP 414

Query: 362 QSGQCIQVSNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLSTDCL 421
            +G CI V     ++ L +C+ S  W   +           LCLKA  +G    L     
Sbjct: 415 STGLCI-VRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFS 474

Query: 422 GKQ-SVWSAISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQ 477
               S W   S+SK+ L++ T++G S+CL +++ N++ IVTNSC C  G+  C  D +SQ
Sbjct: 475 ESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTENNN-IVTNSCKCLRGNSSC--DPRSQ 522

BLAST of Lsi09G002070 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.6e-91
Identity = 179/483 (37.06%), Postives = 281/483 (58.18%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTRYA---NRTIEENFDLLDLKQAKV 60
           ++AEGL+ +S+ +LA + + + FNCVR T+   + T      N T+ ++F  L L     
Sbjct: 34  VVAEGLSKQSVDDLAKKIMAMGFNCVRFTWPLDLATNETLANNVTVRQSFQSLGLNDDIS 93

Query: 61  GLAKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFD 120
           G    NP +++  + EAY+ VV  LG + +MVI DNH+++P WCC  +DGNGFFG+  FD
Sbjct: 94  GFETKNPSMIDLPLIEAYKKVVAKLGNNNVMVILDNHVTKPGWCCGYNDGNGFFGDTFFD 153

Query: 121 PQEWLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLV 180
           P  W+ GL  +A  F+  + VVGMSLRNE+RG  +N +DW  Y+ QG   +H  N  VLV
Sbjct: 154 PTTWIAGLTKIAMTFKGATNVVGMSLRNELRGPKQNVDDWFKYMQQGAEAVHEANPNVLV 213

Query: 181 IVSGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNG 240
           I+SGL+YD DL  ++ + + + T   KLVFE+H YSF+  + + +  +  N  C  I+  
Sbjct: 214 ILSGLSYDTDLSFVRSRHVNL-TFTRKLVFELHRYSFT--NTNTWSSKNPNEACGEILKS 273

Query: 241 FIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSY 300
             +  GF +     FP+F+SE+G D R  +  +NR++ C     A+ D+DW++WT QGSY
Sbjct: 274 IENGGGFNL---RDFPVFLSEFGIDLRGKNVNDNRYIGCILGWAAENDVDWSIWTLQGSY 333

Query: 301 YYREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQC 360
           Y REG    +E +G+LDS W ++++ +F+++  L+ + LQ P S +    +++HP +G C
Sbjct: 334 YLREGVVGMSEFYGILDSDWVRVRSQSFLQRLSLILSPLQGPGSQSKVYNLVFHPLTGLC 393

Query: 361 -IQVSNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKASGEGLEASLS-TDCLGKQ 420
            +Q   D  ++ L  C+ S  WS+    T + + +  LCL+++G      LS T C    
Sbjct: 394 MLQSILDPTKVTLGLCNESQPWSYTPQNT-LTLKDKSLCLESTGPNAPVKLSETSCSSPN 453

Query: 421 -SVWSAISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNG-DPKCLQDTQSQWF 477
            S W  IS S + LA       SLCL ++ +N+  ++ ++C C  G D  C  D  SQWF
Sbjct: 454 LSEWETISASNMLLAA-KSTNNSLCLDVDETNN--LMASNCKCVKGEDSSC--DPISQWF 504

BLAST of Lsi09G002070 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.8e-87
Identity = 173/481 (35.97%), Postives = 270/481 (56.13%), Query Frame = 0

Query: 1   MLAEGLNHRSLKELADEAIKLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKQAKV 60
           ++AEGL+ + +  ++ +   + FNCVRLT+   +    T   N T++++F+   L     
Sbjct: 57  VVAEGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQ 116

Query: 61  GLAKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFD 120
           G+  +NP+++N  +   ++AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+
Sbjct: 117 GIYTHNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFN 176

Query: 121 PQEWLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLV 180
           P  W+ GL  +A  F N   VVGMSLRNE+RG+   + DW  Y+ +G   +H  N  VLV
Sbjct: 177 PDLWMLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLV 236

Query: 181 IVSGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNG 240
           I+SGLN+D DL  LK++P+ + +   KLV E+H YSF+ D   ++    +N+ C+ + + 
Sbjct: 237 ILSGLNFDADLSFLKDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSK 296

Query: 241 FIDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSY 300
                GFV++    FPLF+SE+G DQR  D   NR+M+C  A  A+KDLDWA+W   G Y
Sbjct: 297 ERRTGGFVLD--QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVY 356

Query: 301 YYREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQC 360
           Y+REG+    EA+G+LD++W  + N  ++++  ++Q     P    +    I+HP +G C
Sbjct: 357 YFREGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLC 416

Query: 361 IQVSN--DNKEIFLTNCSTSSRWSHDNDG-TPIRMSNTGLCLKASGEGLEASLSTDCLGK 420
           +   +     E+ L  C+    WS+ + G   IR  +       +  G    L   C   
Sbjct: 417 LVRKSHCHESELTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKI 476

Query: 421 QSVWSAISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWFE 476
           +     IS +K+HL+  T DG  +CL ++S N+  +V NSC C  GD  C  +  SQWF+
Sbjct: 477 EQ----ISATKMHLSFNTSDGSLVCLDVDSDNN--VVANSCNCLTGDTTC--EPASQWFK 525

BLAST of Lsi09G002070 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-69
Identity = 161/483 (33.33%), Postives = 256/483 (53.00%), Query Frame = 0

Query: 2   LAEGLNHRSLKELADEAIKLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKQAKVG 61
           +AEGL+ + L  ++ + + + FNCVRLT+   + T        T++++F+ L L +  +G
Sbjct: 56  VAEGLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLG 115

Query: 62  LAKYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNRNFDP 121
           +  +NP +L+  +  A++ VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP
Sbjct: 116 IQTHNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDP 175

Query: 122 QEWLQGLNLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNNYVTQGVTTIHNINSEVLVI 181
             W +GL  +A  FRN + V+GMSLRNE RG  +  + W  ++ QG   +H  N ++LVI
Sbjct: 176 LVWAKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVI 235

Query: 182 VSGLNYDNDLRCLKEKPLAVNTLDNKLVFEVHLYSFSGDSESKFVQQPLNNICANIMNGF 241
           +SG+++D +L  L+++ + V+  D KLVFE+H YSFS D    + +   N+ C  I+   
Sbjct: 236 LSGIDFDTNLSFLRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKV 295

Query: 242 IDHAGFVMEGSNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYY 301
             + GF++     FPL +SE+G DQR  D + NR+M+C  A  A+ DLDWA+W   G YY
Sbjct: 296 THNGGFLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYY 355

Query: 302 YREGQAEPAEAFGVLDSSWTQIKNPNFVKKFQLLQTMLQDPNSNASFSYVIYHPQSGQCI 361
            R G                                    PN N     +++HP +G C+
Sbjct: 356 LRTGPG--------------------------------LRPNKN-----LLFHPSTGLCV 415

Query: 362 --QVSNDNKEIFLTNCSTSSRWSHDNDGTPIRMSNTGLCLKAS---GEGLEASLSTDCLG 421
               S++   + L  C  S  W+  N    I   N  +C++A    G+ ++  + T C  
Sbjct: 416 TNNPSDNIPTLRLGPCPKSDPWTF-NPSEGILWINK-MCVEAPNVVGQKVKLGVGTKC-- 475

Query: 422 KQSVWSAISNSKLHLATFTQDGKSLCLQIESSNSSKIVTNSCICTNGDPKCLQDTQSQWF 477
             S    IS +K+HL+  T +G  LCL ++  ++S +V N C     D  C  D  SQWF
Sbjct: 476 --SKLGQISATKMHLSFKTSNGLLLCLDVDERDNS-VVANRCKFLTMDASC--DPASQWF 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA01.3e-9839.76Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P545835.4e-1222.32Endoglucanase E1 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351... [more]
P194877.8e-1123.51Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
P235481.5e-0919.47Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7TH832.7e-26490.85Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TMC93.5e-26491.27Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7THG11.5e-26290.64Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TMX01.9e-26290.87Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3D4P48.0e-26191.27Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
KAE8648504.14.2e-26491.68hypothetical protein Csa_008732 [Cucumis sativus][more]
KAA0042710.15.5e-26490.85major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAA0042711.17.2e-26491.27major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
KAE8650477.11.6e-26391.06hypothetical protein Csa_010217 [Cucumis sativus][more]
KAA0042713.13.0e-26290.64major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G13130.12.0e-10239.29Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.16.4e-9339.59Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.6e-9137.06Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.8e-8735.97Glycosyl hydrolase superfamily protein [more]
AT5G16700.11.7e-6933.33Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..337
e-value: 2.6E-57
score: 196.6
NoneNo IPR availableGENE3D2.80.10.50coord: 348..476
e-value: 1.3E-6
score: 30.6
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 1..477
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 10..294
e-value: 4.2E-25
score: 88.7
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 344..475
score: 8.964001
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 4..329
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 344..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G002070.1Lsi09G002070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds