Lsi09G002060 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G002060
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr09: 2152547 .. 2154989 (-)
RNA-Seq ExpressionLsi09G002060
SyntenyLsi09G002060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGGCCAAAATGACAGGAAAAAATATTGCTTTAGCTTGTGTTTTTGTATTGTTAACTTTAAAGGCCTATTCATTGCCTCTTTCAACCAATGGAAGATGGATCGTTGATGCCACAACTGGCCGACGCATGAAGTTGATGTGCGTAAATTGGCCCGGACACATGCAAGCTATGGTGGCAGAGGGTCTTCATCTCCGACCCCTTGATGATATTGCAGCCTCCGTGGCAAAGTTGCGGTTTAGTTGCGTGCGTTTGACATACTCAATCCACATGTTCACACGCTACGCGAATTTGACTGTTCAACAATCCTTTGAGAATTTTGATTTGAAAGATGCCATGGCAGGCATATCCCAAAACAATCCTTCCATATTAAACATGACGTTGATTGAGGCTTATGAAACAGTGGTTGATTCACTTGCTATACATGGAGTCATGGTAGTTTCTGATAATCATATAAGCCAACCAAGATGGTGTTGTAGTGATGATGATGGCAATGGCTTCTTTGGTGATCGCTATTTCGATCCACAAGAATGGCTACAAGGAATTAGTTTGGCAGCTCAAACCCTAAAAAACAAACCCCAAGTATGTTTTAATTATTTATTTCTTTTTTCCTAAAATTTTTAAAACTATAGAAATAGGCCAGGGTTTATGATGTTTAATGCAACAACTTTAAGACTTTCTTTTTAGTTGTTTTAAGGCTAAATTATATGAAAACTTCTCAAAACTAAAAGGCATACATATAATATTTTACAATTTTTTTTTCACGAACCTTATAACATCTAAGCTACACTTTAAATTGTAGTGTTCAAATTTTATTAACACGAGAGGCCAATGAAAGGTATCTTTAGATAATGTGAAAGGTTAAGGGTTATATATAAACTTTTGAAGCTAAATCTAATATTCACATAAACCTTTTACATATTAATTAAAGTTAAAATACTATTTTGGTCACTCTACTTTGTGACTCATTTCATTTTAGTTTCTATACTTTTAAATACACAATTTGAATGCAATTTCAAAATTTATAGAGGGAAGAAAAATATAGACTATTTATTTTTAAAATGTTAATAATAAACTAACAGTCGTAACTAATATTGAAATTTGTGAGAGTACCAGAACTGAATTTGGACAATAGAGATTAAAATATAACATACACAAGTCTTAAAATATAGGGACCAAATTAGTATTTTAATCTGTAATTTAATTATAATTATTGTATATGATATTTTGACCATTTGTATGATACTATATATGCAAAAGGTGGTGGCAATGAGTTTGAGAAACGAACTGCGAGGACCAAACCAAAACGTGGAGGAGTGGTTTCAATATATGAGTCAAGGAGCTAAGCTTATTCACCAAATTAACCCAAATGCTTTAGTAGTGGTTTCTGGATTAAGTTATGACACCGATTTAAGCTTCTTGAAAAATAGGTCGATGGGCTTCAATTTAGACAATAAGCTTGTATTTGAGGCTCACTTGTACTCCTTTACAAACAACATGGGTGATTATTGGACGTCCAAGCCACTAAATACATTTTGTGCTAATGTCAACCAAGGATTTGAAGACCGAGCTGGGTTTCTTGTAAGAGGACAAAACCCAATGCCTCTCTTTGTGAGTGAGTTTGGGATCGACCAAACAGGTGCAAATGAAGGCCAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACCGAGAATGATTTTGATTGGGGACTATGGGCGTTGCAAGGTAGCTACTACTATAGGGAAGGTGTGAAAAATGCTGAGGAAACCTTTGGCGTCTTAGATTCCAAGTTTACAAATGTCAAAAACCCTAAATTCCTTCAGAGGTTTCAGCTTATGCAAACCAAACTTCAAGGTATAGCTTTTAAAGTGATATGTTTCGTTTCCACAATTATTTTCTTGAATTATTTAGGTTTTAAGAAGTATATTAAGTTTCTATCCGCCATTTTTTACACCTTACCTTTTTTTCTCCTTACTATTTGAAGATCAAAATGGGTTTTCTCTTTTAACACAGATCCAAGTTCAAATCTCACCACATCATTCATAATGTACCATCCTTTTAGTGGTGAATGTGTGCGAATGGATAGAAAGTATCAATTAGGAGTTAGTAGTTGTAAGACCTCTAATCGTTGGAGCCATGAAGAAGATGGTGCTCCAATTAAGTTGGCAGGATCTATATTGTGTTTGAAAGCCATTGGAGAAGGGCTCCCTCCAACTCTCTCTAAAGACTGCTCGAGCCAACAAAGTGCTTGGAAATATGCTTCGAATGCAAAGCTTCAACTAGCCACCATTGATCAACAAGGACAAGCATTGTGCTTGCAAAGGGCTTCTCACTTCCATCAAATTGTGACTAACAAGTGTATGTGTCTGAATGATTCTGAATGCCAAGGAGATCCACAAAGTCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAAT

mRNA sequence

CAAGGCCAAAATGACAGGAAAAAATATTGCTTTAGCTTGTGTTTTTGTATTGTTAACTTTAAAGGCCTATTCATTGCCTCTTTCAACCAATGGAAGATGGATCGTTGATGCCACAACTGGCCGACGCATGAAGTTGATGTGCGTAAATTGGCCCGGACACATGCAAGCTATGGTGGCAGAGGGTCTTCATCTCCGACCCCTTGATGATATTGCAGCCTCCGTGGCAAAGTTGCGGTTTAGTTGCGTGCGTTTGACATACTCAATCCACATGTTCACACGCTACGCGAATTTGACTGTTCAACAATCCTTTGAGAATTTTGATTTGAAAGATGCCATGGCAGGCATATCCCAAAACAATCCTTCCATATTAAACATGACGTTGATTGAGGCTTATGAAACAGTGGTTGATTCACTTGCTATACATGGAGTCATGGTAGTTTCTGATAATCATATAAGCCAACCAAGATGGTGTTGTAGTGATGATGATGGCAATGGCTTCTTTGGTGATCGCTATTTCGATCCACAAGAATGGCTACAAGGAATTAGTTTGGCAGCTCAAACCCTAAAAAACAAACCCCAAGTGGTGGCAATGAGTTTGAGAAACGAACTGCGAGGACCAAACCAAAACGTGGAGGAGTGGTTTCAATATATGAGTCAAGGAGCTAAGCTTATTCACCAAATTAACCCAAATGCTTTAGTAGTGGTTTCTGGATTAAGTTATGACACCGATTTAAGCTTCTTGAAAAATAGGTCGATGGGCTTCAATTTAGACAATAAGCTTGTATTTGAGGCTCACTTGTACTCCTTTACAAACAACATGGGTGATTATTGGACGTCCAAGCCACTAAATACATTTTGTGCTAATGTCAACCAAGGATTTGAAGACCGAGCTGGGTTTCTTGTAAGAGGACAAAACCCAATGCCTCTCTTTGTGAGTGAGTTTGGGATCGACCAAACAGGTGCAAATGAAGGCCAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACCGAGAATGATTTTGATTGGGGACTATGGGCGTTGCAAGGTAGCTACTACTATAGGGAAGGTGTGAAAAATGCTGAGGAAACCTTTGGCGTCTTAGATTCCAAGTTTACAAATGTCAAAAACCCTAAATTCCTTCAGAGGTTTCAGCTTATGCAAACCAAACTTCAAGGTATAGCTTTTAAAGTGATATGTTTCGTTTCCACAATTATTTTCTTGAATTATTTAGGTTTTAAGAAATCAAAATGGGTTTTCTCTTTTAACACAGATCCAAGTTCAAATCTCACCACATCATTCATAATGTACCATCCTTTTAGTGGTGAATGTGTGCGAATGGATAGAAAGTATCAATTAGGAGTTAGTAGTTGTAAGACCTCTAATCGTTGGAGCCATGAAGAAGATGGTGCTCCAATTAAGTTGGCAGGATCTATATTGTGTTTGAAAGCCATTGGAGAAGGGCTCCCTCCAACTCTCTCTAAAGACTGCTCGAGCCAACAAAGTGCTTGGAAATATGCTTCGAATGCAAAGCTTCAACTAGCCACCATTGATCAACAAGGACAAGCATTGTGCTTGCAAAGGGCTTCTCACTTCCATCAAATTGTGACTAACAAGTGTATGTGTCTGAATGATTCTGAATGCCAAGGAGATCCACAAAGTCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAAT

Coding sequence (CDS)

ATGACAGGAAAAAATATTGCTTTAGCTTGTGTTTTTGTATTGTTAACTTTAAAGGCCTATTCATTGCCTCTTTCAACCAATGGAAGATGGATCGTTGATGCCACAACTGGCCGACGCATGAAGTTGATGTGCGTAAATTGGCCCGGACACATGCAAGCTATGGTGGCAGAGGGTCTTCATCTCCGACCCCTTGATGATATTGCAGCCTCCGTGGCAAAGTTGCGGTTTAGTTGCGTGCGTTTGACATACTCAATCCACATGTTCACACGCTACGCGAATTTGACTGTTCAACAATCCTTTGAGAATTTTGATTTGAAAGATGCCATGGCAGGCATATCCCAAAACAATCCTTCCATATTAAACATGACGTTGATTGAGGCTTATGAAACAGTGGTTGATTCACTTGCTATACATGGAGTCATGGTAGTTTCTGATAATCATATAAGCCAACCAAGATGGTGTTGTAGTGATGATGATGGCAATGGCTTCTTTGGTGATCGCTATTTCGATCCACAAGAATGGCTACAAGGAATTAGTTTGGCAGCTCAAACCCTAAAAAACAAACCCCAAGTGGTGGCAATGAGTTTGAGAAACGAACTGCGAGGACCAAACCAAAACGTGGAGGAGTGGTTTCAATATATGAGTCAAGGAGCTAAGCTTATTCACCAAATTAACCCAAATGCTTTAGTAGTGGTTTCTGGATTAAGTTATGACACCGATTTAAGCTTCTTGAAAAATAGGTCGATGGGCTTCAATTTAGACAATAAGCTTGTATTTGAGGCTCACTTGTACTCCTTTACAAACAACATGGGTGATTATTGGACGTCCAAGCCACTAAATACATTTTGTGCTAATGTCAACCAAGGATTTGAAGACCGAGCTGGGTTTCTTGTAAGAGGACAAAACCCAATGCCTCTCTTTGTGAGTGAGTTTGGGATCGACCAAACAGGTGCAAATGAAGGCCAAAATCGATTCTTGAGTTGCTTTTTTACCTATCTTACCGAGAATGATTTTGATTGGGGACTATGGGCGTTGCAAGGTAGCTACTACTATAGGGAAGGTGTGAAAAATGCTGAGGAAACCTTTGGCGTCTTAGATTCCAAGTTTACAAATGTCAAAAACCCTAAATTCCTTCAGAGGTTTCAGCTTATGCAAACCAAACTTCAAGGTATAGCTTTTAAAGTGATATGTTTCGTTTCCACAATTATTTTCTTGAATTATTTAGGTTTTAAGAAATCAAAATGGGTTTTCTCTTTTAACACAGATCCAAGTTCAAATCTCACCACATCATTCATAATGTACCATCCTTTTAGTGGTGAATGTGTGCGAATGGATAGAAAGTATCAATTAGGAGTTAGTAGTTGTAAGACCTCTAATCGTTGGAGCCATGAAGAAGATGGTGCTCCAATTAAGTTGGCAGGATCTATATTGTGTTTGAAAGCCATTGGAGAAGGGCTCCCTCCAACTCTCTCTAAAGACTGCTCGAGCCAACAAAGTGCTTGGAAATATGCTTCGAATGCAAAGCTTCAACTAGCCACCATTGATCAACAAGGACAAGCATTGTGCTTGCAAAGGGCTTCTCACTTCCATCAAATTGTGACTAACAAGTGTATGTGTCTGAATGATTCTGAATGCCAAGGAGATCCACAAAGTCAATGGTTTACACTTGTCCCATCAAATGTACATCTCAAT

Protein sequence

MTGKNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRPLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNMTLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTDPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAIGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLNDSECQGDPQSQWFTLVPSNVHLN
Homology
BLAST of Lsi09G002060 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 3.1e-120
Identity = 233/579 (40.24%), Postives = 330/579 (56.99%), Query Frame = 0

Query: 2   TGKNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHL 61
           T   +  A + +L+   ++SLPL T GRWIVD  TG R+KL CVNW GH++  + EGL+ 
Sbjct: 9   TALRLLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNR 68

Query: 62  RPLDDIAASVAKLRFSCVRLTYSIHMFTR--YANLTVQQSFENFDLKDAMAGISQNNPSI 121
            P+  +A +++ L F+CVRLTYSIHM TR  Y N TV Q+F   +L +A +GI  NNP +
Sbjct: 69  LPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPEL 128

Query: 122 LNMTLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGIS 181
           L++  + AY  VV +L+  GVMV+ DNH+S+P+WCC+ DDGNGFFGDRYF+P  W++G+ 
Sbjct: 129 LDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLG 188

Query: 182 LAAQTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDT 241
           L A    N P VVAMSLRNELRG       W ++M  GA  +H+ NP  LV++SGL +DT
Sbjct: 189 LMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDT 248

Query: 242 DLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVR 301
           DLSFL    +      K+V+E H YSF    G  W +   N  C N    F+   GF+  
Sbjct: 249 DLSFLPVLPVTLPFKEKIVYEGHWYSF----GVPWRTGLPNDVCKNETGRFKSNVGFVTS 308

Query: 302 GQN--PMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYR---EG 361
             N    PLF+SEFGIDQ   N+  NR+L+C   YL E D DW LW + GSYYYR   + 
Sbjct: 309 SANATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQP 368

Query: 362 VKNAEETFGVLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSK 421
           VK+ EET+G  +  ++ ++NP F+ R + +Q  +Q                         
Sbjct: 369 VKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQ------------------------- 428

Query: 422 WVFSFNTDPSSNLTTSF-IMYHPFSGECVRMDRKYQLGVSSCKT-SNRWSHEED-GAPIK 481
                  DP       + I+YHP SG CV       + + SC++  +RW+++     PI 
Sbjct: 429 -------DPYLAPGPYYQIIYHPASGLCVESGIGNTVHLGSCQSVRSRWNYDASVKGPIG 488

Query: 482 LAGSILCLKAIGEGLPPTLSKDCSS-QQSAWKYASNAKLQLAT----IDQQGQALCLQRA 541
           L GS  C+   G GLP  ++++CS+   + W   S+A+LQL T     D + + +CL   
Sbjct: 489 LMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTVSSAQLQLGTRVLGKDGKEKWMCLD-G 548

Query: 542 SHFHQIVTNKCMCLNDSEC--QGDPQSQWFTLVPSNVHL 564
           S    I TN+C+C+ DS C  + +P+ QWF ++ +N  L
Sbjct: 549 SKSPLISTNECICITDSHCYPKLNPEKQWFKVITTNKQL 550

BLAST of Lsi09G002060 vs. ExPASy TrEMBL
Match: A0A5A7TI19 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00580 PE=3 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 2.2e-273
Identity = 458/562 (81.49%), Postives = 493/562 (87.72%), Query Frame = 0

Query: 4   KNIALACVFV-LLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLR 63
           KNIAL CVFV LLT KAYSLPLSTNGRWI+DATTGRR+KLMCVNW GHMQ M+ EGLH R
Sbjct: 8   KNIALVCVFVLLLTFKAYSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGMLVEGLHRR 67

Query: 64  PLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNM 123
           PLDDIAA VAKLRF+CVRLTYSIHMFTR+AN+TV+QSFENFD+KDA+ GI+QNNPSILN+
Sbjct: 68  PLDDIAALVAKLRFNCVRLTYSIHMFTRHANVTVKQSFENFDMKDALVGIAQNNPSILNL 127

Query: 124 TLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAA 183
           TL+EAY  VVDSLA HG+MVVSDNHISQPRWCC +DDGNGFFGDRYFDPQEW QGISLAA
Sbjct: 128 TLVEAYGAVVDSLAAHGIMVVSDNHISQPRWCCDNDDGNGFFGDRYFDPQEWFQGISLAA 187

Query: 184 QTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 243
           Q+LK+K QVVAMSLRNE RGPNQNVE+WFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS
Sbjct: 188 QSLKSKAQVVAMSLRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 247

Query: 244 FLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQN 303
           FL+NRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTFCA++NQGFEDRAGFLVRGQN
Sbjct: 248 FLRNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVRGQN 307

Query: 304 PMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETF 363
           P+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAEE F
Sbjct: 308 PIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAEENF 367

Query: 364 GVLDSKFTNVKNPK-FLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNT 423
           GVLDS FT  KN K FLQRFQLMQTKLQ                                
Sbjct: 368 GVLDSNFTKAKNSKLFLQRFQLMQTKLQ-------------------------------- 427

Query: 424 DPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKA 483
           DPSSN TTSFIMYHP SG C+RM++KYQLG+SSCKTSNRWSHE+DGAPIKLAGSILCLKA
Sbjct: 428 DPSSNFTTSFIMYHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 487

Query: 484 IGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLN 543
           IG GLPP LS+DCSSQQS WKY SNAKLQLAT+D+QGQALCLQRASH HQIVTNKC+C N
Sbjct: 488 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 537

Query: 544 DSECQGDPQSQWFTLVPSNVHL 564
           DS+CQ DPQSQWFTLVPSN+ L
Sbjct: 548 DSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G002060 vs. ExPASy TrEMBL
Match: A0A1S3CT43 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504654 PE=3 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 2.2e-273
Identity = 459/560 (81.96%), Postives = 494/560 (88.21%), Query Frame = 0

Query: 4   KNIALACVFV-LLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLR 63
           KNIAL CVFV LLT KA+SLPLSTNGRWI+DATTGRR+KLMCVNW GHMQ M+ EGLH R
Sbjct: 8   KNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGMLVEGLHRR 67

Query: 64  PLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNM 123
           PLDDIAA VAKLRF+CVRLTYSIHMFTR+ANLTV+QSFENFD+KDAMAGI+QNNPSILN+
Sbjct: 68  PLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQNNPSILNL 127

Query: 124 TLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAA 183
           TL+EAY  VVDSL  HG+MVVSDNHISQPRWCC ++DGNGFFGDRYFDPQEWLQGISLAA
Sbjct: 128 TLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWLQGISLAA 187

Query: 184 QTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 243
           Q+LK+K QVVAMSLRNELRGPNQNVE+WFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS
Sbjct: 188 QSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 247

Query: 244 FLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQN 303
           FLKNRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTFCA++NQGFEDRAGFLVRGQN
Sbjct: 248 FLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVRGQN 307

Query: 304 PMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETF 363
           P+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAEE F
Sbjct: 308 PIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAEENF 367

Query: 364 GVLDSKFTNVKNPK-FLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNT 423
           GVLDS FT  KN K FLQRFQLMQTKLQ                                
Sbjct: 368 GVLDSNFTKAKNSKLFLQRFQLMQTKLQ-------------------------------- 427

Query: 424 DPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKA 483
           DPSSN TT+FIMYHP SG CVRM++KYQLG+SSCKTSNRWSHE+DGAPIKLAGSILCLKA
Sbjct: 428 DPSSNFTTTFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 487

Query: 484 IGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLN 543
           IG GLPP LS+DCSSQQS W+YASNAKLQLAT+D+QGQALCLQRASH HQIVTNKC+C  
Sbjct: 488 IGVGLPPILSQDCSSQQSIWRYASNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCTI 535

Query: 544 DSECQGDPQSQWFTLVPSNV 562
           DS+CQ DPQSQWFTLVPSN+
Sbjct: 548 DSQCQEDPQSQWFTLVPSNL 535

BLAST of Lsi09G002060 vs. ExPASy TrEMBL
Match: A0A0A0KL32 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171770 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.7e-273
Identity = 458/562 (81.49%), Postives = 494/562 (87.90%), Query Frame = 0

Query: 4   KNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRP 63
           KNIAL CVFVLLT KAYSLPLSTNGRWIVDATTG+R+KLMCVNWPGHMQ M+AEGLH RP
Sbjct: 8   KNIALVCVFVLLTFKAYSLPLSTNGRWIVDATTGQRVKLMCVNWPGHMQGMLAEGLHRRP 67

Query: 64  LDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNMT 123
           LDDI + VAKLRF+CVRLTYSIHMFTR+ANLTVQQSFENFD+KDAMAGI+QNNPS++N+T
Sbjct: 68  LDDIISLVAKLRFNCVRLTYSIHMFTRHANLTVQQSFENFDMKDAMAGIAQNNPSLVNLT 127

Query: 124 LIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQ 183
           L+EAY  VVDSLA HGVMVVSDNHISQPRWCC++DDGNGFFGDRYFDP+EWLQGISLAAQ
Sbjct: 128 LVEAYGAVVDSLAAHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPEEWLQGISLAAQ 187

Query: 184 TLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSF 243
           +LK+K +VVAMS+RNE RGPNQNVE+WFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSF
Sbjct: 188 SLKSKAEVVAMSMRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSF 247

Query: 244 LKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNP 303
           LKNRSMGFNLDNKLVFEAHLYSFTNNMGD+W SKPLNTFCA+VNQGFEDRAGFLVRGQNP
Sbjct: 248 LKNRSMGFNLDNKLVFEAHLYSFTNNMGDFWMSKPLNTFCASVNQGFEDRAGFLVRGQNP 307

Query: 304 MPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFG 363
           MPLFVSEFGIDQ G NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYYREGVKNAEE FG
Sbjct: 308 MPLFVSEFGIDQRGVNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYREGVKNAEENFG 367

Query: 364 VLDSKFTNVKNPK-FLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTD 423
           VLDS F   KN K FLQRFQLMQTKLQ                                D
Sbjct: 368 VLDSTFAKAKNSKLFLQRFQLMQTKLQ--------------------------------D 427

Query: 424 PSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAI 483
           PSSN TTS IMYHP SG CVRM++KYQLG+SSCKTSNRW HE+D +PIKLAGS+LCLKAI
Sbjct: 428 PSSNFTTSLIMYHPLSGGCVRMNKKYQLGISSCKTSNRWIHEQDSSPIKLAGSVLCLKAI 487

Query: 484 GEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQR-ASHFHQIVTNKCMCLN 543
           G GLPP LS+DCSSQQS WKY S+AKLQLAT+D+QGQALCLQR ASH HQIVTNKC+C N
Sbjct: 488 GVGLPPILSQDCSSQQSIWKYGSSAKLQLATVDEQGQALCLQRAASHSHQIVTNKCLCSN 537

Query: 544 DSECQGDPQSQWFTLVPSNVHL 564
           DS+CQ DPQSQWFTLVPSN+ L
Sbjct: 548 DSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G002060 vs. ExPASy TrEMBL
Match: A0A5D3BM14 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00610 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 455/560 (81.25%), Postives = 492/560 (87.86%), Query Frame = 0

Query: 4   KNIALACVFV-LLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLR 63
           KNIAL CVFV LLT KA+SLPLSTNGRWI+DATTG+R+KLMCVNW GHMQ M+ EGLH R
Sbjct: 8   KNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGQRVKLMCVNWAGHMQGMLVEGLHRR 67

Query: 64  PLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNM 123
           PLDDIAA VAKLRF+CVRLTYSIHMFTR+ANLTV+QSFENFD+KDAMAGI+QNNPSILN+
Sbjct: 68  PLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQNNPSILNL 127

Query: 124 TLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAA 183
           TL+EAY  VVDSL  HG+MVVSDNHISQPRWCC ++DGNGFFGDRYFDPQEW QGISLAA
Sbjct: 128 TLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWFQGISLAA 187

Query: 184 QTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 243
           Q+LK+K QVVAMSLRNELRGPNQNVE+WFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS
Sbjct: 188 QSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 247

Query: 244 FLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQN 303
           FLKNRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTFCA++NQGFEDRAGFLVRGQN
Sbjct: 248 FLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVRGQN 307

Query: 304 PMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETF 363
           P+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAEE F
Sbjct: 308 PIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAEENF 367

Query: 364 GVLDSKFTNVKNPK-FLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNT 423
           GVLDS FT  KN K FLQRFQLMQTKLQ                                
Sbjct: 368 GVLDSNFTKAKNSKLFLQRFQLMQTKLQ-------------------------------- 427

Query: 424 DPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKA 483
           DP+SN TT+FIMYHP SG CVRM++KYQLG+SSCKTSNRWSHE+D APIKLAGSILCLKA
Sbjct: 428 DPNSNFTTTFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDSAPIKLAGSILCLKA 487

Query: 484 IGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLN 543
           IG GLPP LS+DCSSQQS W+YASNAKLQLAT+D+QGQALCLQRASH HQIVTNKC+C  
Sbjct: 488 IGVGLPPILSQDCSSQQSIWRYASNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCTI 535

Query: 544 DSECQGDPQSQWFTLVPSNV 562
           DS+CQ DPQSQWFTLVPSN+
Sbjct: 548 DSQCQEDPQSQWFTLVPSNL 535

BLAST of Lsi09G002060 vs. ExPASy TrEMBL
Match: A0A5A7UJG7 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00790 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 1.0e-270
Identity = 454/560 (81.07%), Postives = 491/560 (87.68%), Query Frame = 0

Query: 4   KNIALACVFV-LLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLR 63
           KNIAL CVFV LLT KA+SLPLSTNGRWI+DATTG+R+KLMCVNW GHMQ M+ EGLH R
Sbjct: 8   KNIALVCVFVLLLTFKAFSLPLSTNGRWIIDATTGQRVKLMCVNWAGHMQGMLVEGLHRR 67

Query: 64  PLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNM 123
           PLDDIAA VAKLRF+CVRLTYSIHMFTR+ANLTV+QSFENFD+KDAMAGI+QNNPSILN+
Sbjct: 68  PLDDIAALVAKLRFNCVRLTYSIHMFTRHANLTVKQSFENFDMKDAMAGIAQNNPSILNL 127

Query: 124 TLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAA 183
           TL+EAY  VVDSL  HG+MVVSDNHISQPRWCC ++DGNGFFGDRYFDPQEW QGISLAA
Sbjct: 128 TLVEAYGAVVDSLVAHGIMVVSDNHISQPRWCCDNNDGNGFFGDRYFDPQEWFQGISLAA 187

Query: 184 QTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 243
           Q+LK+K QVVAMSLRNELRGPNQNVE+WFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS
Sbjct: 188 QSLKSKAQVVAMSLRNELRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 247

Query: 244 FLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQN 303
           FLKNRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTFCA++NQGFEDRAGFLVRGQN
Sbjct: 248 FLKNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVRGQN 307

Query: 304 PMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETF 363
           P+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAEE F
Sbjct: 308 PIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAEENF 367

Query: 364 GVLDSKFTNVKNPK-FLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNT 423
           GVLDS FT  KN K FLQRFQLMQTKLQ                                
Sbjct: 368 GVLDSNFTKAKNSKLFLQRFQLMQTKLQ-------------------------------- 427

Query: 424 DPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKA 483
           DP+SN TT+FIMYHP SG CVRM++KYQLG+SSCKTSNRWSHE+D APIKLAGSILCLKA
Sbjct: 428 DPNSNFTTTFIMYHPLSGGCVRMNKKYQLGISSCKTSNRWSHEQDSAPIKLAGSILCLKA 487

Query: 484 IGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLN 543
           IG GLPP LS+DCSSQQS W+YASNAKLQLAT+D+QGQALCLQRASH HQIVTNKC+C  
Sbjct: 488 IGVGLPPILSQDCSSQQSIWRYASNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCTI 535

Query: 544 DSECQGDPQSQWFTLVPSNV 562
           DS+CQ DPQSQWF LVPSN+
Sbjct: 548 DSQCQEDPQSQWFILVPSNL 535

BLAST of Lsi09G002060 vs. NCBI nr
Match: KAE8648503.1 (hypothetical protein Csa_009407 [Cucumis sativus])

HSP 1 Score: 996.9 bits (2576), Expect = 7.1e-287
Identity = 477/567 (84.13%), Postives = 512/567 (90.30%), Query Frame = 0

Query: 1   MTG---KNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAE 60
           MTG   KN+ALACVFVLLT KAYSLPLSTNGRWIV+ATTG+R+KLMCVNWPGHMQAMVAE
Sbjct: 1   MTGQPWKNLALACVFVLLTFKAYSLPLSTNGRWIVEATTGQRVKLMCVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNP 120
           GLHLRPLDDIA  VA LRF+CVRLTYSIHMFTRYANLTV+QSFENFD+K+A+AGI+QNNP
Sbjct: 61  GLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNP 120

Query: 121 SILNMTLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQG 180
           +ILNM ++EAYE VVDSL  HGVMVVSDNHISQPRWCCS+DDGNGFFGDRYF+ QEWL+G
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEG 180

Query: 181 ISLAAQTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSY 240
           +SLAAQ+LKNKPQVVAMSLRNE RGPNQNVE WFQYMSQGAKL+HQINPNALVVVSGLSY
Sbjct: 181 LSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFL 300
           DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM DYWTSKPLNTFCANVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 VRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
           VRGQNP+PLFVSEFGI+Q GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVF 420
            EETFGVLD+KFTNVKNPKFLQ+FQLMQTKLQ                            
Sbjct: 361 DEETFGVLDAKFTNVKNPKFLQKFQLMQTKLQ---------------------------- 420

Query: 421 SFNTDPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSIL 480
               DPSSNLTTSFIMYHP SGECV++++KYQLGVSSCKTSNRWSHE+DG PIKL GSIL
Sbjct: 421 ----DPSSNLTTSFIMYHPLSGECVQVNKKYQLGVSSCKTSNRWSHEQDGTPIKLVGSIL 480

Query: 481 CLKAIGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKC 540
           CL+A+G+GLPP LSKDCSSQQSAWKYASNAKLQLAT+D++GQALCLQRASH HQI+TNKC
Sbjct: 481 CLQAVGDGLPPILSKDCSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKC 535

Query: 541 MCLNDSECQGDPQSQWFTLVPSNVHLN 565
           MC NDSECQGDPQSQWFTLVPSNVHLN
Sbjct: 541 MCPNDSECQGDPQSQWFTLVPSNVHLN 535

BLAST of Lsi09G002060 vs. NCBI nr
Match: KAE8648505.1 (hypothetical protein Csa_007897 [Cucumis sativus])

HSP 1 Score: 992.6 bits (2565), Expect = 1.3e-285
Identity = 476/564 (84.40%), Postives = 509/564 (90.25%), Query Frame = 0

Query: 1   MTG---KNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAE 60
           MTG   KN+ALACVFVLLT KAYSLPLSTNGRWIV+ATTG+R+KLMCVNWPGHMQAMVAE
Sbjct: 1   MTGQPWKNLALACVFVLLTFKAYSLPLSTNGRWIVEATTGQRVKLMCVNWPGHMQAMVAE 60

Query: 61  GLHLRPLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNP 120
           GLHLRPLDDIA  VA LRF+CVRLTYSIHMFTRYANLTV+QSFENFD+K+A+AGI+QNNP
Sbjct: 61  GLHLRPLDDIATMVANLRFNCVRLTYSIHMFTRYANLTVKQSFENFDMKEAIAGIAQNNP 120

Query: 121 SILNMTLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQG 180
           +ILNM ++EAYE VVDSL  HGVMVVSDNHISQPRWCCS+DDGNGFFGDRYF+ QEWL+G
Sbjct: 121 TILNMKVVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLEG 180

Query: 181 ISLAAQTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSY 240
           +SLAAQ+LKNKPQVVAMSLRNE RGPNQNVE WFQYMSQGAKL+HQINPNALVVVSGLSY
Sbjct: 181 LSLAAQSLKNKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLVHQINPNALVVVSGLSY 240

Query: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFL 300
           DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNM DYWTSKPLNTFCANVNQGFEDRAGFL
Sbjct: 241 DTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMSDYWTSKPLNTFCANVNQGFEDRAGFL 300

Query: 301 VRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360
           VRGQNP+PLFVSEFGI+Q GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN
Sbjct: 301 VRGQNPIPLFVSEFGINQMGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKN 360

Query: 361 AEETFGVLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVF 420
            EETFGVLDSKFTNVKNPKFLQ+FQLMQTKLQ                            
Sbjct: 361 DEETFGVLDSKFTNVKNPKFLQKFQLMQTKLQ---------------------------- 420

Query: 421 SFNTDPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSIL 480
               DPSSNLTTSFIMYHP SGECVRM++KYQLGVSSCKTSNRWSHE+DG PIKL GSIL
Sbjct: 421 ----DPSSNLTTSFIMYHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDGTPIKLVGSIL 480

Query: 481 CLKAIGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKC 540
           CL+A+G+GLPP LSKDCSSQQSAWKYASNAKLQLAT+D++GQALCLQRASH HQI+TNKC
Sbjct: 481 CLQAVGDGLPPILSKDCSSQQSAWKYASNAKLQLATVDEEGQALCLQRASHSHQILTNKC 532

Query: 541 MCLNDSECQGDPQSQWFTLVPSNV 562
           MC NDSECQGDPQSQWFTLVPSN+
Sbjct: 541 MCPNDSECQGDPQSQWFTLVPSNL 532

BLAST of Lsi09G002060 vs. NCBI nr
Match: XP_031739082.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical protein Csa_010408 [Cucumis sativus])

HSP 1 Score: 989.2 bits (2556), Expect = 1.5e-284
Identity = 471/561 (83.96%), Postives = 506/561 (90.20%), Query Frame = 0

Query: 4   KNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRP 63
           KN++LACVFVLLT +AYSLPLSTNGRWIV+ATTG+R+KL+CVNWPGHMQAMVAEGLHL+P
Sbjct: 7   KNVSLACVFVLLTFEAYSLPLSTNGRWIVEATTGQRVKLICVNWPGHMQAMVAEGLHLKP 66

Query: 64  LDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNMT 123
           LDDIAA V KLRF+CVRLTYSIHMFTRYANLTV+QSFENFDLK+A+ GI+QNNP+ILNM 
Sbjct: 67  LDDIAAMVVKLRFNCVRLTYSIHMFTRYANLTVKQSFENFDLKEAIVGIAQNNPTILNMK 126

Query: 124 LIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQ 183
           ++EAYE VVDSL  HGVMVVSDNHISQPRWCCS+DDGNGFFGDRYF+ QEWLQG+SLA Q
Sbjct: 127 VVEAYEAVVDSLGAHGVMVVSDNHISQPRWCCSNDDGNGFFGDRYFNSQEWLQGLSLATQ 186

Query: 184 TLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSF 243
           +LK KPQVVAMSLRNE RGPNQNVE WFQYMSQG KL+HQINPNALVVVSGLSYDTDLSF
Sbjct: 187 SLKTKPQVVAMSLRNEPRGPNQNVEMWFQYMSQGTKLVHQINPNALVVVSGLSYDTDLSF 246

Query: 244 LKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNP 303
           LKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNP
Sbjct: 247 LKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNP 306

Query: 304 MPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFG 363
           +PLFVSEFGI+Q G NEGQNRFLSCFFTYLT+NDFDWGLWALQGSYYYREGVKN EETFG
Sbjct: 307 IPLFVSEFGINQMGVNEGQNRFLSCFFTYLTKNDFDWGLWALQGSYYYREGVKNDEETFG 366

Query: 364 VLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTDP 423
           VLDSKFTNVKNPKFLQ+FQLMQTKLQ                                DP
Sbjct: 367 VLDSKFTNVKNPKFLQKFQLMQTKLQ--------------------------------DP 426

Query: 424 SSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAIG 483
           SSNLTTSFIMYHP SGECVRM++KYQLGVSSCKTSNRWSHE+D  PIKLAGSILCL+A+G
Sbjct: 427 SSNLTTSFIMYHPLSGECVRMNKKYQLGVSSCKTSNRWSHEQDDTPIKLAGSILCLQAVG 486

Query: 484 EGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLNDS 543
           +GLPP LSKDCSSQQSAWKYASNAKLQLAT+D+QGQALCLQRASH HQI+TNKCMC NDS
Sbjct: 487 DGLPPILSKDCSSQQSAWKYASNAKLQLATVDEQGQALCLQRASHSHQILTNKCMCPNDS 535

Query: 544 ECQGDPQSQWFTLVPSNVHLN 565
           ECQGDPQSQWFTLVPSNVHL+
Sbjct: 547 ECQGDPQSQWFTLVPSNVHLS 535

BLAST of Lsi09G002060 vs. NCBI nr
Match: XP_038874277.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 964.9 bits (2493), Expect = 3.0e-277
Identity = 465/561 (82.89%), Postives = 496/561 (88.41%), Query Frame = 0

Query: 4   KNIALACVFVLLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRP 63
           KNIAL C+FVLLT KAYSLPLSTNGRWIVDA TG+RMKLMCVNWPGHMQ M+AEGLHLRP
Sbjct: 7   KNIALVCIFVLLTFKAYSLPLSTNGRWIVDAATGQRMKLMCVNWPGHMQVMLAEGLHLRP 66

Query: 64  LDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNMT 123
           LDDIA  VAKLRF+CVRLTYSIHMFTRYANLTVQQSFENFDLK+AMAGI++NNPSILNMT
Sbjct: 67  LDDIAVLVAKLRFNCVRLTYSIHMFTRYANLTVQQSFENFDLKEAMAGIARNNPSILNMT 126

Query: 124 LIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQ 183
           LIEAY+ VVDSLA+HGVMVVSDNHISQPRWCC++DDGNGFFGDRYFDPQEWLQGISLAAQ
Sbjct: 127 LIEAYKAVVDSLAVHGVMVVSDNHISQPRWCCNNDDGNGFFGDRYFDPQEWLQGISLAAQ 186

Query: 184 TLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSF 243
           +LK+K QVVAMSLRNE RGPNQNVE WFQYMSQGAKLIHQ NP+ALVV+SGLSYDTDLSF
Sbjct: 187 SLKSKFQVVAMSLRNEPRGPNQNVEMWFQYMSQGAKLIHQNNPDALVVISGLSYDTDLSF 246

Query: 244 LKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNP 303
           LKNRS+GFNL+NKLVFEAHLYSFTNNM D WTSKPLNTFCANVNQGFEDRAGFLVRGQNP
Sbjct: 247 LKNRSLGFNLNNKLVFEAHLYSFTNNMSDSWTSKPLNTFCANVNQGFEDRAGFLVRGQNP 306

Query: 304 MPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFG 363
            PLFVSEFGIDQTG N+GQNR+LSCFFTYL ENDFDWGLWALQGSYYYREGVKNAEE+FG
Sbjct: 307 TPLFVSEFGIDQTGKNDGQNRYLSCFFTYLVENDFDWGLWALQGSYYYREGVKNAEESFG 366

Query: 364 VLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTDP 423
           V D  F + KNPKFLQRFQLMQTKLQ                                DP
Sbjct: 367 VSDFNFRHAKNPKFLQRFQLMQTKLQ--------------------------------DP 426

Query: 424 SSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAIG 483
           SSNLT SFIMYH  SGECVRMD+KYQL VSSCKTSNRW+H+EDGAPIKL  S+LCLKA+G
Sbjct: 427 SSNLTASFIMYHSLSGECVRMDKKYQLRVSSCKTSNRWNHKEDGAPIKLVDSLLCLKALG 486

Query: 484 EGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLNDS 543
           EGLPP LSKDCSSQQS WKYASNAKLQLAT+D+QGQALCLQ  SH HQIVTNKCMCL+DS
Sbjct: 487 EGLPPILSKDCSSQQSVWKYASNAKLQLATVDKQGQALCLQSVSHSHQIVTNKCMCLDDS 535

Query: 544 ECQGDPQSQWFTLVPSNVHLN 565
           +CQGDPQSQWFTLV SNV+LN
Sbjct: 547 QCQGDPQSQWFTLVFSNVNLN 535

BLAST of Lsi09G002060 vs. NCBI nr
Match: KAA0042984.1 (endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 951.0 bits (2457), Expect = 4.5e-273
Identity = 458/562 (81.49%), Postives = 493/562 (87.72%), Query Frame = 0

Query: 4   KNIALACVFV-LLTLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLR 63
           KNIAL CVFV LLT KAYSLPLSTNGRWI+DATTGRR+KLMCVNW GHMQ M+ EGLH R
Sbjct: 8   KNIALVCVFVLLLTFKAYSLPLSTNGRWIIDATTGRRVKLMCVNWAGHMQGMLVEGLHRR 67

Query: 64  PLDDIAASVAKLRFSCVRLTYSIHMFTRYANLTVQQSFENFDLKDAMAGISQNNPSILNM 123
           PLDDIAA VAKLRF+CVRLTYSIHMFTR+AN+TV+QSFENFD+KDA+ GI+QNNPSILN+
Sbjct: 68  PLDDIAALVAKLRFNCVRLTYSIHMFTRHANVTVKQSFENFDMKDALVGIAQNNPSILNL 127

Query: 124 TLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAA 183
           TL+EAY  VVDSLA HG+MVVSDNHISQPRWCC +DDGNGFFGDRYFDPQEW QGISLAA
Sbjct: 128 TLVEAYGAVVDSLAAHGIMVVSDNHISQPRWCCDNDDGNGFFGDRYFDPQEWFQGISLAA 187

Query: 184 QTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 243
           Q+LK+K QVVAMSLRNE RGPNQNVE+WFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS
Sbjct: 188 QSLKSKAQVVAMSLRNEPRGPNQNVEKWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLS 247

Query: 244 FLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQN 303
           FL+NRSMGFNLDNKLVFEAHLYSFTNNM D+W SKPLNTFCA++NQGFEDRAGFLVRGQN
Sbjct: 248 FLRNRSMGFNLDNKLVFEAHLYSFTNNMRDFWMSKPLNTFCASINQGFEDRAGFLVRGQN 307

Query: 304 PMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETF 363
           P+PLFVSEFGIDQTG NEGQNRFLSCFF+YLTENDFDWGLWALQGSYYY+ GVKNAEE F
Sbjct: 308 PIPLFVSEFGIDQTGTNEGQNRFLSCFFSYLTENDFDWGLWALQGSYYYKVGVKNAEENF 367

Query: 364 GVLDSKFTNVKNPK-FLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNT 423
           GVLDS FT  KN K FLQRFQLMQTKLQ                                
Sbjct: 368 GVLDSNFTKAKNSKLFLQRFQLMQTKLQ-------------------------------- 427

Query: 424 DPSSNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKA 483
           DPSSN TTSFIMYHP SG C+RM++KYQLG+SSCKTSNRWSHE+DGAPIKLAGSILCLKA
Sbjct: 428 DPSSNFTTSFIMYHPLSGGCMRMNKKYQLGISSCKTSNRWSHEQDGAPIKLAGSILCLKA 487

Query: 484 IGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLN 543
           IG GLPP LS+DCSSQQS WKY SNAKLQLAT+D+QGQALCLQRASH HQIVTNKC+C N
Sbjct: 488 IGVGLPPILSQDCSSQQSIWKYGSNAKLQLATVDEQGQALCLQRASHSHQIVTNKCLCSN 537

Query: 544 DSECQGDPQSQWFTLVPSNVHL 564
           DS+CQ DPQSQWFTLVPSN+ L
Sbjct: 548 DSQCQEDPQSQWFTLVPSNLRL 537

BLAST of Lsi09G002060 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 434.9 bits (1117), Expect = 1.0e-121
Identity = 235/557 (42.19%), Postives = 321/557 (57.63%), Query Frame = 0

Query: 11  VFVL-LTLKAYSLPLSTNGRWIV-DATTGRRMKLMCVNWPGHMQAMVAEGLHLRPLDDIA 70
           VF+L   +  ++ P ST+ RWIV D   GRR+KL CVNWP H++  VAEGL  +PLD IA
Sbjct: 9   VFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSKQPLDAIA 68

Query: 71  ASVAKLRFSCVRLTYSIHMFTR---YANLTVQQSFENFDLKDAMAGISQNNPSILNMTLI 130
             +  + F+CVRLT+ +++ T     A +TV+QS   F L +A++G   +NP+IL++ LI
Sbjct: 69  EKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPTILDLPLI 128

Query: 131 EAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQTL 190
           +A++ VV  L  H VMV+ DNHISQP WCCSD+DGNGFFGD++ +PQ W++G+   A   
Sbjct: 129 KAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGLKKMASMF 188

Query: 191 KN-KPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFL 250
            N    VV MSLRNELRGP QN+++W++YM +GA+ +H +NPN LV+VSGL+Y TDLSFL
Sbjct: 189 ANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNYATDLSFL 248

Query: 251 KNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNPM 310
           + R    +   K+VFE H Y F N     W    LN  C    +     +GFL+  +  +
Sbjct: 249 RERPFEVSFRRKVVFEIHWYGFWNT----WEGDNLNKICGKETEKMMKMSGFLL--EKGI 308

Query: 311 PLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGV 370
           PLFVSEFGIDQ G N   N+FLSCF     + D DW LW L GSYY RE    ++E++GV
Sbjct: 309 PLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKSIGSDESYGV 368

Query: 371 LDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTDPS 430
           LD  +++++N   LQ    +QT   G+                             T P 
Sbjct: 369 LDFNWSSIRNSTILQMISAIQTPFIGL---------------------------METQPK 428

Query: 431 SNLTTSFIMYHPFSGECVRMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAIGE 490
                  IM+HP +G C+     +QL + SC  S  W              ILCLKA  +
Sbjct: 429 K------IMFHPSTGLCIVRKSLFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEK 488

Query: 491 GLPPTLSKDCS-SQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCL-ND 550
           G    L    S S  S WK  S++K+QL++I + G ++CL   +  + IVTN C CL  +
Sbjct: 489 GKSVKLRLFFSESYCSKWKLFSDSKMQLSSITKNGFSVCLDVDTENNNIVTNSCKCLRGN 524

Query: 551 SECQGDPQSQWFTLVPS 560
           S C  DP+SQWF LV S
Sbjct: 549 SSC--DPRSQWFKLVTS 524

BLAST of Lsi09G002060 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.9e-120
Identity = 224/544 (41.18%), Postives = 312/544 (57.35%), Query Frame = 0

Query: 23  PLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRPLDDIAASVAKLRFSCVRLT 82
           PLSTN RWI+D   G+R+KL CVNWP H+Q +VAEGL  + +DD+A  +  + F+CVR T
Sbjct: 4   PLSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 83  YSIHMFTRYA---NLTVQQSFENFDLKDAMAGISQNNPSILNMTLIEAYETVVDSLAIHG 142
           + + + T      N+TV+QSF++  L D ++G    NPS++++ LIEAY+ VV  L  + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 143 VMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQTLKNKPQVVAMSLRNE 202
           VMV+ DNH+++P WCC  +DGNGFFGD +FDP  W+ G++  A T K    VV MSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 203 LRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKLVF 262
           LRGP QNV++WF+YM QGA+ +H+ NPN LV++SGLSYDTDLSF+++R +      KLVF
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNLTFTRKLVF 243

Query: 263 EAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNPMPLFVSEFGIDQTGAN 322
           E H YSFTN   + W+SK  N  C  + +  E+  GF +R     P+F+SEFGID  G N
Sbjct: 244 ELHRYSFTNT--NTWSSKNPNEACGEILKSIENGGGFNLR---DFPVFLSEFGIDLRGKN 303

Query: 323 EGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSKFTNVKNPKFLQ 382
              NR++ C   +  END DW +W LQGSYY REGV    E +G+LDS +  V++  FLQ
Sbjct: 304 VNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFLQ 363

Query: 383 RFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTDPSSNLTTSFIMYHPFSG 442
           R  L+ + LQG                                P S      +++HP +G
Sbjct: 364 RLSLILSPLQG--------------------------------PGSQSKVYNLVFHPLTG 423

Query: 443 ECVRMD----RKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAIGEGLPPTLSK-DC 502
            C+        K  LG+  C  S  WS+      + L    LCL++ G   P  LS+  C
Sbjct: 424 LCMLQSILDPTKVTLGL--CNESQPWSYTPQNT-LTLKDKSLCLESTGPNAPVKLSETSC 483

Query: 503 SSQQ-SAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLNDSECQGDPQSQW 558
           SS   S W+  S + + LA       +LCL      + ++ + C C+   +   DP SQW
Sbjct: 484 SSPNLSEWETISASNMLLAA-KSTNNSLCLD-VDETNNLMASNCKCVKGEDSSCDPISQW 504

BLAST of Lsi09G002060 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 429.9 bits (1104), Expect = 3.2e-120
Identity = 221/549 (40.26%), Postives = 317/549 (57.74%), Query Frame = 0

Query: 16  TLKAYSLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRPLDDIAASVAKLR 75
           T+   S PLST+ RWIVD   G R+KL+C NWP H+Q +VAEGL  +P+D +A  + ++ 
Sbjct: 27  TVPNMSYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMG 86

Query: 76  FSCVRLTYSIHMFTRYA---NLTVQQSFENFDLKDAMAGISQNNPSILNMTLIEAYETVV 135
           F+CVRLT+ + + T      N+TV+QSF++  L D + G   NNPSI+++ LIEAY+TVV
Sbjct: 87  FNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVV 146

Query: 136 DSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQTLKNKPQVV 195
            +L  + VMV+ DNH+++P WCC++DDGNGFFGD++FDP  W+  +   A T      VV
Sbjct: 147 TTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVV 206

Query: 196 AMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGFN 255
            MSLRNELRGP QNV +WF+YM QGA+ +H  N   LV++SGLS+D DLSF+++R +  +
Sbjct: 207 GMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLS 266

Query: 256 LDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLVRGQNPMPLFVSEFG 315
              KLVFE H YSF++  G+ W +   N  C  V     +  G+L+      PLF+SEFG
Sbjct: 267 FTGKLVFELHWYSFSD--GNSWAANNPNDICGRVLNRIGNGGGYLL--NQGFPLFLSEFG 326

Query: 316 IDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREGVKNAEETFGVLDSKFTNV 375
           ID+ G N   NR+  C   +  END DW LWAL GSYY R+G     E +GVLDS + +V
Sbjct: 327 IDERGVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISV 386

Query: 376 KNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSKWVFSFNTDPSSNLTTSFI 435
           +N  FLQ+   +Q+ LQG                                P        +
Sbjct: 387 RNSSFLQKISFLQSPLQG--------------------------------PGPRTDAYNL 446

Query: 436 MYHPFSGECV--RMDRKYQLGVSSCKTSNRWSHEEDGAPIKLAGSILCLKAIGEGLPPTL 495
           ++HP +G C+   +D    L +  C +S  WS+ +    IK     LCL++ G   P T+
Sbjct: 447 VFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKKALRIK--DQQLCLQSNGPKNPVTM 506

Query: 496 SK-DCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASHFHQIVTNKCMCLN-DSECQGD 555
           ++  CS+  S W+  S +++ LA+      +LCL      + +V N C CL+ D  C  +
Sbjct: 507 TRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLD-VDTANNVVANACKCLSKDKSC--E 533

Query: 556 PQSQWFTLV 558
           P SQWF ++
Sbjct: 567 PMSQWFKII 533

BLAST of Lsi09G002060 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.8e-99
Identity = 210/569 (36.91%), Postives = 301/569 (52.90%), Query Frame = 0

Query: 4   KNIALACVFVLLTLKAYSL------PLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAE 63
           K IAL  VF+ L L   SL      PL T  RWIV+   G R+KL C NWP H++ +VAE
Sbjct: 3   KAIALT-VFLFLFLSLISLTLATDYPLFTKSRWIVN-NKGHRVKLACANWPSHLKPVVAE 62

Query: 64  GLHLRPLDDIAASVAKLRFSCVRLTYSIHMF---TRYANLTVQQSFENFDLKDAMAGISQ 123
           GL  +P+D I+  +  + F+CVRLT+ + +    T   N+TV+QSFE + L   + GI  
Sbjct: 63  GLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYT 122

Query: 124 NNPSILNMTLIEAYETVVDSLAIHGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEW 183
           +NP I+N  LI  ++ VV SL  H VMV+ DNH + P WCCS+DD + FFGD  F+P  W
Sbjct: 123 HNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLW 182

Query: 184 LQGISLAAQTLKNKPQVVAMSLRNELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSG 243
           + G+   A    N   VV MSLRNELRG N   ++W++YM +GA+ +H  NPN LV++SG
Sbjct: 183 MLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSG 242

Query: 244 LSYDTDLSFLKNRSMGFNLDNKLVFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRA 303
           L++D DLSFLK+R +  +   KLV E H YSFT+  G  W S  +N FC+ +        
Sbjct: 243 LNFDADLSFLKDRPVNLSFKKKLVLELHWYSFTDGTGQ-WKSHNVNDFCSQMFSKERRTG 302

Query: 304 GFLVRGQNPMPLFVSEFGIDQTGANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREG 363
           GF++      PLF+SEFG DQ G +   NR+++C   +  E D DW +WA+ G YY+REG
Sbjct: 303 GFVL--DQGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREG 362

Query: 364 VKNAEETFGVLDSKFTNVKNPKFLQRFQLMQTKLQGIAFKVICFVSTIIFLNYLGFKKSK 423
            +   E +G+LD+ + NV N  +L+R  ++Q                             
Sbjct: 363 KRGVVEAYGMLDANWHNVHNYTYLRRLSVIQPP--------------------------- 422

Query: 424 WVFSFNTDPSSNLTTSFIMYHPFSGEC-VRMD--RKYQLGVSSCKTSNRWSHEEDGAPIK 483
                +T P         ++HP +G C VR     + +L +  C     WS+   G    
Sbjct: 423 -----HTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESELTLGPCTKDEPWSYSHGGILEI 482

Query: 484 LAGSILCLK---AIGEGLPPTLSKDCSSQQSAWKYASNAKLQLATIDQQGQALCLQRASH 543
             G   CL+   A+G+ +   L + C+  +      S  K+ L+     G  +CL   S 
Sbjct: 483 RRGHKSCLEGETAVGKSV--KLGRICTKIEQ----ISATKMHLSFNTSDGSLVCLDVDSD 525

Query: 544 FHQIVTNKCMCL-NDSECQGDPQSQWFTL 557
            + +V N C CL  D+ C  +P SQWF +
Sbjct: 543 -NNVVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of Lsi09G002060 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 9.8e-85
Identity = 151/338 (44.67%), Postives = 212/338 (62.72%), Query Frame = 0

Query: 21  SLPLSTNGRWIVDATTGRRMKLMCVNWPGHMQAMVAEGLHLRPLDDIAASVAKLRFSCVR 80
           S PLST  RWIVD   G+R+KL CVNWP H+Q  VAEGL  +PLD I+  +  + F+CVR
Sbjct: 23  SYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAEGLSKQPLDSISKKIVSMGFNCVR 82

Query: 81  LTYSIHMFTR---YANLTVQQSFENFDLKDAMAGISQNNPSILNMTLIEAYETVVDSLAI 140
           LT+ + + T       +TV+QSFE+  L + + GI  +NP +L++ L  A++ VV +L  
Sbjct: 83  LTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLPLFNAFQEVVSNLGE 142

Query: 141 HGVMVVSDNHISQPRWCCSDDDGNGFFGDRYFDPQEWLQGISLAAQTLKNKPQVVAMSLR 200
           +GVMV+ DNH++ P WCC D+D + FFG  +FDP  W +G+   A   +N   V+ MSLR
Sbjct: 143 NGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMATLFRNFTHVIGMSLR 202

Query: 201 NELRGPNQNVEEWFQYMSQGAKLIHQINPNALVVVSGLSYDTDLSFLKNRSMGFNLDNKL 260
           NE RG     + WF++M QGA+ +H  NP  LV++SG+ +DT+LSFL++RS+  +  +KL
Sbjct: 203 NEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSFLRDRSVNVSFTDKL 262

Query: 261 VFEAHLYSFTNNMGDYWTSKPLNTFCANVNQGFEDRAGFLV-RGQNPMPLFVSEFGIDQT 320
           VFE H YSF++   D W     N FC  + +      GFL+ RG    PL +SEFG DQ 
Sbjct: 263 VFELHWYSFSDGR-DSWRKHNSNDFCVKIIEKVTHNGGFLLGRG---FPLILSEFGTDQR 322

Query: 321 GANEGQNRFLSCFFTYLTENDFDWGLWALQGSYYYREG 355
           G +   NR+++C   +  END DW +WAL G YY R G
Sbjct: 323 GGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C0HLA03.1e-12040.24Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TI192.2e-27381.49Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A1S3CT432.2e-27381.96Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504654 PE=3 SV... [more]
A0A0A0KL323.7e-27381.49Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G171770 PE=3... [more]
A0A5D3BM142.0e-27181.25Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7UJG71.0e-27081.07Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
KAE8648503.17.1e-28784.13hypothetical protein Csa_009407 [Cucumis sativus][more]
KAE8648505.11.3e-28584.40hypothetical protein Csa_007897 [Cucumis sativus][more]
XP_031739082.11.5e-28483.96glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8650476.1 hypothetical... [more]
XP_038874277.13.0e-27782.89glycosyl hydrolase 5 family protein-like [Benincasa hispida][more]
KAA0042984.14.5e-27381.49endoglucanase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G26130.11.0e-12142.19Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.9e-12041.18Cellulase (glycosyl hydrolase family 5) protein [more]
AT1G13130.13.2e-12040.26Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.8e-9936.91Glycosyl hydrolase superfamily protein [more]
AT5G16700.19.8e-8544.67Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 63..346
e-value: 2.4E-21
score: 76.4
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 22..387
e-value: 4.1E-69
score: 235.5
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 5..392
coord: 421..558
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 23..378

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G002060.1Lsi09G002060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds