Lsi09G001630 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G001630
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING-type domain-containing protein
Locationchr09: 1707890 .. 1710656 (-)
RNA-Seq ExpressionLsi09G001630
SyntenyLsi09G001630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAACCTCCAAATGGGCTTAATTAATTAGCTTCTGTTTTGTTGACAAAGTGACATTATTTTAACAAAATCCTCCTCTCGTTTTCTGACCTTAAATTGAAACAACTTCCTCTCTATAAGCAAACCAAGAAGCTTTCTTTCTTTCTTTTCTTTCTGTGTTCAAATTTGTGCTTGAGTTTGGGTTTAACCTTATAATTTCTTGTTTGTTTTTTGTTTGATGGGTTTGCCTCAAACTCCATCCCCTCCTCATCTTTACCCTCAAGCACTTCAACTTAAGCTTTATCAAGCCTTCATATTCTCCATTCCAATCCTCTTCTCCATTATTCTCTTCCTTCTTTTCTACTTGTTTTATCTCAAAAGAAGAGCTTCCTCTCTCTCTTCTTCTTCATCTTCATCTCCCCCAATTCTTCCAACTACTTCCAATACACTCACTCTCTATGTTACCACTGTAAGCAAATTATTATTCCTCTCTTTCTCAATCTTCCATTTTTCCTTTCATGTTTTTTTTTGGTGTTTCCATTGCTGTAATTTGCTTTTGAGGGGTCAGGGAAAATCTAAGCAAAAAAAGTTGAAGCTTTAATATTCATCTTTCTTTTTATTGATTTGTTTTGCCATTTTTCTTCATCTCATTGATCTTAATTGTTCAGGCTTGTCAAGTGGATTTGAAGGTAGATAATCCCATAGACAAACTTCCCAAGATCCAATTCAATGAAGAACTAAAGACTAGAGATTCTCTGTAAGTATCACCATTTCTTATTGTTAACAAAACAAAAACTCTGTTGGCTTTTTCGGGTTCTTTTGAAAGCAAAAAGGTTGTTCATGAACAGCTGTTCCTCTAAAATAAATGGGTTTGGAATACTGAATTTTTCCAAAATTTGCCAATTGATCCCTTTGCCACCCACATGCACAAATTGATATTAGATTACATGAATTCAAGAGTTTTTTTTCTAAAGGGAAGTATCTATGATATAAACTAATGCAAAATATACTTTTGGTCCCTGAAGTTTGAAGATGATGTCTATTTGATCTCTAAAGTTTAAAAACAGCATTTTTAGTCCCTAAAGTTTTAAAGATAATTCTAAATGGTCCCTAAAGTCAATTAAATGTTAACTGACTAATGGAAAGATGATGTGGTCATTTTAGAATATGATTACATAGCAAAATGAGATGAGTTCTTCTTAACCCTAAACTAATAGATGCACCAAAATATCTCTACTAGAATAACACGAAGTTAACATCGGTCCACTAAAACAGGTATTAATGTGTTACATTTTCATTGCACAAAAAATTTTATTTTTTCCTTTATTGAGTTCATCAATTATCATAATGGAAGAACGAACTATCAATTTTTAGAATGATAAGTAAGATTTTATTCGTTGAGCTGTATCCGAATTGATACAGAAAAAGCTGGAAAGAGAGAAAAGAAAAATACAAAACAGTCAAGGGATAGGGATGGAAGATTTAGTGACTTAAATAGGGAGCACACATAACAAACAATATAAAGATAAAAGTGAAACTGATCCTTTTTCAATTTTTGTTGATTTTTTTAGATTACTTTTCTTTGGAGGATTGGAATCTCTTATAACATCCGCTCTTTAATTTCTCAAAAGTGGCTTTTAAATTCTTGGTTCTAAAAGTTACTTTTGCATTTGTTTTCTTTTCTTCTTGAAAGATGTTGTTTGTAGAACCTTAGGCCGACAAAATTGATAATGAAGTTGAAGAGCATGTTTGATTTCTATTTTTTTTTTTAATTTTTTTTTCTCTTTCTATAGTAATTATGTTGCAATCTTATTTCTAAAGTTTATTCTTTATTGTTGTATTACTTTAATAAATAGTTTGATGAAGTAATTTAAATCTCATTTTTGAGCACTACTACTAGATGGCCAAGTTTGTTATTAATTTTAAACTCTTATTTCTCTTCACACGGATCGGTCTTTTTTGTTTATGAATCCAAATTTCTCATTTCTTTGGTCTACTTTTGTACATCAATGGGATTTATGACCTAGCATAAATCTAGTAGGTAGGTATCCCAATGAGCCTAGAAAAATTTCTAATGCTACGAGAATATCACACTCTCCTTGTTCACACTATAAATTTTTCTCATAATTAACTCCACATTAATGTCAGTAGTTGCAGTCTCATTCTATTTATAAAAACTTGTTGATGTAGCATACAATAAATAAAAGAAACACGAGCCTCTCTAGCCTCTCTTCATAGTGTGTTCAACAAATGTATAGGATGAAAGATTTGAACATTTGACCTCTTGATCATAAATATATGTCTTAACTAATTGAGTTGTTTTTTTCTCTCGCCGCTTGTAATTCAATTTCTCCCATTTGTGGGAGAGAAAGATTGAGATTTCAAGTCAACCTTATATTGACCTTAGGATATGTGGGGTTAGAAATGAACATTGAAACAAAGAAAAAAGCAACAACTTTCTTATCCATTTAACAGGTGCTGCGTCTGCCTTGGAGAATTCGAGATAAAAGAGGAATTGTTGCAAGTCCCATCATGCAAACATGTGTTTCATATTGACTGTATGAACCACTGGCTCATCTCCAACTCAACTTGTCCTCTCTGTAGATGCTCAGTCATCTCAACAACCCGATGTTCTGATCTGCCCGTCATCGCACCGCCCCCGCCACCGCCACTGGCTGGCTCATCAGAGCTTCCCCTGACTTCCAACCAGACTGCAGAGATGGGATCATATTCACTCCAACATCTGCAGCTGCAACAACAGGAAGGATCAAGTCGGAGTATATGCTAA

mRNA sequence

AAAAAACCTCCAAATGGGCTTAATTAATTAGCTTCTGTTTTGTTGACAAAGTGACATTATTTTAACAAAATCCTCCTCTCGTTTTCTGACCTTAAATTGAAACAACTTCCTCTCTATAAGCAAACCAAGAAGCTTTCTTTCTTTCTTTTCTTTCTGTGTTCAAATTTGTGCTTGAGTTTGGGTTTAACCTTATAATTTCTTGTTTGTTTTTTGTTTGATGGGTTTGCCTCAAACTCCATCCCCTCCTCATCTTTACCCTCAAGCACTTCAACTTAAGCTTTATCAAGCCTTCATATTCTCCATTCCAATCCTCTTCTCCATTATTCTCTTCCTTCTTTTCTACTTGTTTTATCTCAAAAGAAGAGCTTCCTCTCTCTCTTCTTCTTCATCTTCATCTCCCCCAATTCTTCCAACTACTTCCAATACACTCACTCTCTATGTTACCACTGCTTGTCAAGTGGATTTGAAGGTAGATAATCCCATAGACAAACTTCCCAAGATCCAATTCAATGAAGAACTAAAGACTAGAGATTCTCTGTGCTGCGTCTGCCTTGGAGAATTCGAGATAAAAGAGGAATTGTTGCAAGTCCCATCATGCAAACATGTGTTTCATATTGACTGTATGAACCACTGGCTCATCTCCAACTCAACTTGTCCTCTCTGTAGATGCTCAGTCATCTCAACAACCCGATGTTCTGATCTGCCCGTCATCGCACCGCCCCCGCCACCGCCACTGGCTGGCTCATCAGAGCTTCCCCTGACTTCCAACCAGACTGCAGAGATGGGATCATATTCACTCCAACATCTGCAGCTGCAACAACAGGAAGGATCAAGTCGGAGTATATGCTAA

Coding sequence (CDS)

ATGGGTTTGCCTCAAACTCCATCCCCTCCTCATCTTTACCCTCAAGCACTTCAACTTAAGCTTTATCAAGCCTTCATATTCTCCATTCCAATCCTCTTCTCCATTATTCTCTTCCTTCTTTTCTACTTGTTTTATCTCAAAAGAAGAGCTTCCTCTCTCTCTTCTTCTTCATCTTCATCTCCCCCAATTCTTCCAACTACTTCCAATACACTCACTCTCTATGTTACCACTGCTTGTCAAGTGGATTTGAAGGTAGATAATCCCATAGACAAACTTCCCAAGATCCAATTCAATGAAGAACTAAAGACTAGAGATTCTCTGTGCTGCGTCTGCCTTGGAGAATTCGAGATAAAAGAGGAATTGTTGCAAGTCCCATCATGCAAACATGTGTTTCATATTGACTGTATGAACCACTGGCTCATCTCCAACTCAACTTGTCCTCTCTGTAGATGCTCAGTCATCTCAACAACCCGATGTTCTGATCTGCCCGTCATCGCACCGCCCCCGCCACCGCCACTGGCTGGCTCATCAGAGCTTCCCCTGACTTCCAACCAGACTGCAGAGATGGGATCATATTCACTCCAACATCTGCAGCTGCAACAACAGGAAGGATCAAGTCGGAGTATATGCTAA

Protein sequence

MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELPLTSNQTAEMGSYSLQHLQLQQQEGSSRSIC
Homology
BLAST of Lsi09G001630 vs. ExPASy Swiss-Prot
Match: Q84TF5 (Probable E3 ubiquitin-protein ligase RHA4A OS=Arabidopsis thaliana OX=3702 GN=RHA4A PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-49
Identity = 108/159 (67.92%), Postives = 131/159 (82.39%), Query Frame = 0

Query: 4   PQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPI 63
           PQ+P  PHLYPQA+QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL   SS SP I
Sbjct: 4   PQSPITPHLYPQAIQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL---SSPSPMI 63

Query: 64  LPTTSNTLT-LYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELL 123
           LP +S+  T  ++ + C +D+KV+   DKL  + FNEEL TRDSLCCVCLGEFE+KEEL+
Sbjct: 64  LPVSSSHQTSSHLPSVCLLDVKVELK-DKLHVVLFNEELGTRDSLCCVCLGEFELKEELV 123

Query: 124 QVPSCKHVFHIDCMNHWLISNSTCPLCRCSV-ISTTRCS 161
           ++P CKH+FH+DC++ WL S++TCPLCR SV IS+T+ S
Sbjct: 124 EMPLCKHIFHLDCIHLWLYSHNTCPLCRSSVSISSTKTS 158

BLAST of Lsi09G001630 vs. ExPASy Swiss-Prot
Match: Q570X5 (RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-23
Identity = 59/137 (43.07%), Postives = 87/137 (63.50%), Query Frame = 0

Query: 18  QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTT 77
           +LKLYQAFIFS+PI F+ I+  LFYL YL+R +S LSS    +  I   + +T+ L ++ 
Sbjct: 19  ELKLYQAFIFSVPICFTFIILFLFYLIYLRRSSSDLSSLGMRTTFIPGNSLSTIELGLSK 78

Query: 78  ACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMN 137
             +         + LP + F E     DS C VCLG+++  ++L Q+P CKH FH+DC++
Sbjct: 79  ELR---------EMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFHMDCID 138

Query: 138 HWLISNSTCPLCRCSVI 155
            WL S++TCPLCR ++I
Sbjct: 139 LWLTSHTTCPLCRLALI 146

BLAST of Lsi09G001630 vs. ExPASy Swiss-Prot
Match: Q9SN28 (RING-H2 finger protein ATL7 OS=Arabidopsis thaliana OX=3702 GN=ATL7 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-21
Identity = 60/156 (38.46%), Postives = 97/156 (62.18%), Query Frame = 0

Query: 3   LPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASS---LSSSSSS 62
           +P+T +P +   ++ +LKLYQAFIFS+PI F+ I+  + Y+ YL+R +++    SS    
Sbjct: 11  IPETYAPSN-STESEKLKLYQAFIFSVPICFTFIVLFVLYVIYLRRNSTTNVDWSSLGMR 70

Query: 63  SPPILPTTSNTLTLYVTTACQVDLKVDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIK 122
               +PT +N  T         +L +   I + LP + + E    +DS C VCLG+++ +
Sbjct: 71  GGTFVPTNNNLST--------AELGLSKDIREMLPVVIYKESFIVKDSQCSVCLGDYQAE 130

Query: 123 EELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVI 155
           E+L Q+PSC H FH++C++ WL S++TCPLCR S+I
Sbjct: 131 EKLQQMPSCGHTFHMECIDLWLTSHTTCPLCRLSLI 157

BLAST of Lsi09G001630 vs. ExPASy Swiss-Prot
Match: Q9SN27 (E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana OX=3702 GN=ATL59 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 48/131 (36.64%), Postives = 76/131 (58.02%), Query Frame = 0

Query: 25  FIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTTACQVDLK 84
           FI  +PI   +I+ L+ Y+    RR S+ +   SS    +PT +N  T         +L 
Sbjct: 24  FIVCVPICVILIVLLVLYIM---RRNSNTNVDWSSLGGFVPTNNNLST--------AELG 83

Query: 85  VDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLISN 144
           +   I + LP + + E     D+ C VCLG+++ +E+L Q+PSC H FH++C++ WL S+
Sbjct: 84  LSKDIREMLPIVIYKESFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLWLTSH 143

Query: 145 STCPLCRCSVI 155
           +TCPLCR S+I
Sbjct: 144 TTCPLCRLSLI 143

BLAST of Lsi09G001630 vs. ExPASy Swiss-Prot
Match: Q8GW38 (RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.1e-12
Identity = 54/141 (38.30%), Postives = 80/141 (56.74%), Query Frame = 0

Query: 26  IFSIPILFSIILFL-----LFYLFYLKRRASSLSSS---SSSSPPILPTTSNTLTLYVTT 85
           I  I +L S+I F+     L   +YLK++ S+LSSS   S+ +P    + S+T    +  
Sbjct: 53  ILFIIVLLSVIFFICSILHLLVRYYLKKKRSNLSSSPNESNQNPEF--SDSDTYQRQLQQ 112

Query: 86  ACQV-DLKVDNP-IDKLPKIQFNEELKTRDSL-CCVCLGEFEIKEELLQVPSCKHVFHID 145
              + D  +D   ID LP   + E   T++   C VCL EF   ++L  +P+C H FHID
Sbjct: 113 LFHLHDSGLDQALIDALPVFLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHAFHID 172

Query: 146 CMNHWLISNSTCPLCRCSVIS 156
           C++ WL+SNSTCPLCR ++ S
Sbjct: 173 CIDTWLLSNSTCPLCRGTLFS 191

BLAST of Lsi09G001630 vs. ExPASy TrEMBL
Match: A0A6J1EM64 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111435669 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.5e-98
Identity = 192/214 (89.72%), Postives = 197/214 (92.06%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC D PV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPPPPPPAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           ELPLTSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 ELPLTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

BLAST of Lsi09G001630 vs. ExPASy TrEMBL
Match: A0A6J1I2V6 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111469090 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.2e-96
Identity = 190/214 (88.79%), Postives = 196/214 (91.59%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC DLPV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPPPPPSAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           E P TS+QTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 EPPPTSDQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

BLAST of Lsi09G001630 vs. ExPASy TrEMBL
Match: A0A6J1H2A4 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111459379 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.8e-93
Identity = 188/219 (85.84%), Postives = 196/219 (89.50%), Query Frame = 0

Query: 1   MGLPQTP-SPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQTP +PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTPAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  S-PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIK 120
           S PPILPTTSN LTLYVTTAC+VDLK DNP+DKLPKI FNEEL++RDSLCCVCLGEFEIK
Sbjct: 61  SPPPILPTTSNRLTLYVTTACRVDLKADNPMDKLPKIFFNEELRSRDSLCCVCLGEFEIK 120

Query: 121 EELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIA-------PPPPP 180
           EELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVI TTRCSD PV+        PPPPP
Sbjct: 121 EELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPPPPP 180

Query: 181 PLAGSSELPLTSNQTAEMGSYSLQHLQLQQQEGSSRSIC 211
           PLA S ELPL SNQTAEM SYSLQH QLQQQ GSS+SIC
Sbjct: 181 PLAASLELPLASNQTAEMVSYSLQHPQLQQQIGSSQSIC 219

BLAST of Lsi09G001630 vs. ExPASy TrEMBL
Match: A0A6J1DWP0 (probable E3 ubiquitin-protein ligase RHA4A OS=Momordica charantia OX=3673 GN=LOC111023829 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.4e-92
Identity = 182/210 (86.67%), Postives = 189/210 (90.00%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MGLPQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLS SSSSS
Sbjct: 1   MGLPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSFSSSSS 60

Query: 61  PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEE 120
           PPILPT+SN LTLYVTTACQVD K DNPI+KLPKI FNEEL+ RDSLCCVCLGEFEIKEE
Sbjct: 61  PPILPTSSNALTLYVTTACQVDSKADNPIEKLPKILFNEELRARDSLCCVCLGEFEIKEE 120

Query: 121 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELP 180
           LLQVPSCKHVFHIDCMNHWL +NSTCPLCRCSVI   RC D PV AP P  PL   SELP
Sbjct: 121 LLQVPSCKHVFHIDCMNHWLTTNSTCPLCRCSVIPPIRCPDPPVAAPSPTLPL--PSELP 180

Query: 181 LTSNQTAEMGSYSLQHLQLQQQEGSSRSIC 211
           LTSNQTAEM SYSL+H QLQQQEGSS+SIC
Sbjct: 181 LTSNQTAEMVSYSLRHPQLQQQEGSSQSIC 208

BLAST of Lsi09G001630 vs. ExPASy TrEMBL
Match: A0A6J1K5R3 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111490976 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.1e-92
Identity = 187/218 (85.78%), Postives = 195/218 (89.45%), Query Frame = 0

Query: 1   MGLPQT-PSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60
           MG+PQT  +PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS
Sbjct: 1   MGVPQTSAAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSS 60

Query: 61  S--PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
           S  PPILPTTSN LTLYVTTAC+VDLK +NPIDKLPKI FNEEL++RDSLCCVCLGEFEI
Sbjct: 61  SPPPPILPTTSNRLTLYVTTACRVDLKANNPIDKLPKIFFNEELRSRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPV-----IAPPPPPP 180
           KEELLQVPSCKHVFHIDCMN WL SNSTCPLCRCSVI TTRCSD PV     + PPPPPP
Sbjct: 121 KEELLQVPSCKHVFHIDCMNLWLASNSTCPLCRCSVIPTTRCSDPPVLVPPQLTPPPPPP 180

Query: 181 LAGSSELPLTSNQTAEMGSYSLQHLQLQQQEGSSRSIC 211
           LA S ELPL SNQTAEM SYSLQH QLQQQ GSS+SIC
Sbjct: 181 LAASLELPLASNQTAEMVSYSLQHPQLQQQIGSSQSIC 218

BLAST of Lsi09G001630 vs. NCBI nr
Match: XP_038907033.1 (probable E3 ubiquitin-protein ligase RHA4A [Benincasa hispida])

HSP 1 Score: 376.7 bits (966), Expect = 1.3e-100
Identity = 198/212 (93.40%), Postives = 202/212 (95.28%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEE 120
           PPILPTTSNTLTLYVTTACQVDLK DN I+KLPKIQF+EELKTRDSLCCVCLGEFEIKE+
Sbjct: 61  PPILPTTSNTLTLYVTTACQVDLKADNLIEKLPKIQFDEELKTRDSLCCVCLGEFEIKEQ 120

Query: 121 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELP 180
           LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVI TTRCSD P IA  PPPPLAGSSELP
Sbjct: 121 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVIPTTRCSDPPGIA--PPPPLAGSSELP 180

Query: 181 LTSNQTAEMGSYSLQHLQL--QQQEGSSRSIC 211
            TSNQTAEMGSYSLQHLQL  QQQEGSS+SIC
Sbjct: 181 PTSNQTAEMGSYSLQHLQLQQQQQEGSSQSIC 210

BLAST of Lsi09G001630 vs. NCBI nr
Match: XP_022928899.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata])

HSP 1 Score: 366.7 bits (940), Expect = 1.3e-97
Identity = 192/214 (89.72%), Postives = 197/214 (92.06%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC D PV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPPPPPPAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           ELPLTSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 ELPLTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

BLAST of Lsi09G001630 vs. NCBI nr
Match: XP_022970098.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita maxima])

HSP 1 Score: 362.5 bits (929), Expect = 2.5e-96
Identity = 190/214 (88.79%), Postives = 196/214 (91.59%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  ---PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEI 120
              PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEI
Sbjct: 61  SSFPPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEI 120

Query: 121 KEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSS 180
           KEELLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC DLPV+  PPPPP AGSS
Sbjct: 121 KEELLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPPPPPSAGSS 180

Query: 181 ELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           E P TS+QTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 EPPPTSDQTAEMRSYSLLHPQLQQQQEGSSQSIC 214

BLAST of Lsi09G001630 vs. NCBI nr
Match: XP_023550207.1 (probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023550208.1 probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 9.6e-96
Identity = 189/218 (86.70%), Postives = 196/218 (89.91%), Query Frame = 0

Query: 1   MGLPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS 60
           MG PQTP+PPHLYPQALQLKLYQAFIFSIPIL SIILFLLFYLFYLKRRASSLSSSSSSS
Sbjct: 1   MGQPQTPAPPHLYPQALQLKLYQAFIFSIPILVSIILFLLFYLFYLKRRASSLSSSSSSS 60

Query: 61  -------PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLG 120
                  PPILPTTSNTLTLY+TTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLG
Sbjct: 61  SSSSSSFPPILPTTSNTLTLYITTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLG 120

Query: 121 EFEIKEELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPL 180
           EFEIKEELLQVPSCKHVFHIDC+NHWL SNSTCPLCRCSVI TTRC DLPV+  PPPPP 
Sbjct: 121 EFEIKEELLQVPSCKHVFHIDCINHWLTSNSTCPLCRCSVIPTTRCFDLPVVVSPPPPPP 180

Query: 181 AGSSELPLTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
           AGSSELP TSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 181 AGSSELPPTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 218

BLAST of Lsi09G001630 vs. NCBI nr
Match: KAG6579565.1 (30S ribosomal protein S6 alpha, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-95
Identity = 189/211 (89.57%), Postives = 194/211 (91.94%), Query Frame = 0

Query: 4   PQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS--- 63
           PQTP+PPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSS   
Sbjct: 192 PQTPAPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSSSF 251

Query: 64  PPILPTTSNTLTLYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEE 123
           PPILPTTSNTLTLYVTTACQVDLK DNP+DKLPKI FNEEL+TRDSLCCVCLGEFEIKEE
Sbjct: 252 PPILPTTSNTLTLYVTTACQVDLKADNPVDKLPKILFNEELRTRDSLCCVCLGEFEIKEE 311

Query: 124 LLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVISTTRCSDLPVIAPPPPPPLAGSSELP 183
           LLQVPSCKHVFHIDCMNHWL SNSTCPLCRCSVI TTRC D PV+  PPPPP AGSSELP
Sbjct: 312 LLQVPSCKHVFHIDCMNHWLTSNSTCPLCRCSVIPTTRCFDPPVVVSPPPPPPAGSSELP 371

Query: 184 LTSNQTAEMGSYSLQHLQL-QQQEGSSRSIC 211
            TSNQTAEM SYSL H QL QQQEGSS+SIC
Sbjct: 372 PTSNQTAEMRSYSLLHPQLQQQQEGSSQSIC 402

BLAST of Lsi09G001630 vs. TAIR 10
Match: AT4G24015.1 (RING/U-box superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 108/159 (67.92%), Postives = 131/159 (82.39%), Query Frame = 0

Query: 4   PQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPI 63
           PQ+P  PHLYPQA+QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL   SS SP I
Sbjct: 4   PQSPITPHLYPQAIQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSL---SSPSPMI 63

Query: 64  LPTTSNTLT-LYVTTACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELL 123
           LP +S+  T  ++ + C +D+KV+   DKL  + FNEEL TRDSLCCVCLGEFE+KEEL+
Sbjct: 64  LPVSSSHQTSSHLPSVCLLDVKVELK-DKLHVVLFNEELGTRDSLCCVCLGEFELKEELV 123

Query: 124 QVPSCKHVFHIDCMNHWLISNSTCPLCRCSV-ISTTRCS 161
           ++P CKH+FH+DC++ WL S++TCPLCR SV IS+T+ S
Sbjct: 124 EMPLCKHIFHLDCIHLWLYSHNTCPLCRSSVSISSTKTS 158

BLAST of Lsi09G001630 vs. TAIR 10
Match: AT1G33480.1 (RING/U-box superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 9.5e-25
Identity = 59/137 (43.07%), Postives = 87/137 (63.50%), Query Frame = 0

Query: 18  QLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTT 77
           +LKLYQAFIFS+PI F+ I+  LFYL YL+R +S LSS    +  I   + +T+ L ++ 
Sbjct: 19  ELKLYQAFIFSVPICFTFIILFLFYLIYLRRSSSDLSSLGMRTTFIPGNSLSTIELGLSK 78

Query: 78  ACQVDLKVDNPIDKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMN 137
             +         + LP + F E     DS C VCLG+++  ++L Q+P CKH FH+DC++
Sbjct: 79  ELR---------EMLPIVVFKESFTVMDSQCSVCLGDYQPNDKLQQIPVCKHTFHMDCID 138

Query: 138 HWLISNSTCPLCRCSVI 155
            WL S++TCPLCR ++I
Sbjct: 139 LWLTSHTTCPLCRLALI 146

BLAST of Lsi09G001630 vs. TAIR 10
Match: AT4G10150.1 (RING/U-box superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 60/156 (38.46%), Postives = 97/156 (62.18%), Query Frame = 0

Query: 3   LPQTPSPPHLYPQALQLKLYQAFIFSIPILFSIILFLLFYLFYLKRRASS---LSSSSSS 62
           +P+T +P +   ++ +LKLYQAFIFS+PI F+ I+  + Y+ YL+R +++    SS    
Sbjct: 11  IPETYAPSN-STESEKLKLYQAFIFSVPICFTFIVLFVLYVIYLRRNSTTNVDWSSLGMR 70

Query: 63  SPPILPTTSNTLTLYVTTACQVDLKVDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIK 122
               +PT +N  T         +L +   I + LP + + E    +DS C VCLG+++ +
Sbjct: 71  GGTFVPTNNNLST--------AELGLSKDIREMLPVVIYKESFIVKDSQCSVCLGDYQAE 130

Query: 123 EELLQVPSCKHVFHIDCMNHWLISNSTCPLCRCSVI 155
           E+L Q+PSC H FH++C++ WL S++TCPLCR S+I
Sbjct: 131 EKLQQMPSCGHTFHMECIDLWLTSHTTCPLCRLSLI 157

BLAST of Lsi09G001630 vs. TAIR 10
Match: AT4G10160.1 (RING/U-box superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-15
Identity = 48/131 (36.64%), Postives = 76/131 (58.02%), Query Frame = 0

Query: 25  FIFSIPILFSIILFLLFYLFYLKRRASSLSSSSSSSPPILPTTSNTLTLYVTTACQVDLK 84
           FI  +PI   +I+ L+ Y+    RR S+ +   SS    +PT +N  T         +L 
Sbjct: 24  FIVCVPICVILIVLLVLYIM---RRNSNTNVDWSSLGGFVPTNNNLST--------AELG 83

Query: 85  VDNPI-DKLPKIQFNEELKTRDSLCCVCLGEFEIKEELLQVPSCKHVFHIDCMNHWLISN 144
           +   I + LP + + E     D+ C VCLG+++ +E+L Q+PSC H FH++C++ WL S+
Sbjct: 84  LSKDIREMLPIVIYKESFTVNDTQCSVCLGDYQAEEKLQQMPSCGHTFHMECIDLWLTSH 143

Query: 145 STCPLCRCSVI 155
           +TCPLCR S+I
Sbjct: 144 TTCPLCRLSLI 143

BLAST of Lsi09G001630 vs. TAIR 10
Match: AT1G23980.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.2e-13
Identity = 54/141 (38.30%), Postives = 80/141 (56.74%), Query Frame = 0

Query: 26  IFSIPILFSIILFL-----LFYLFYLKRRASSLSSS---SSSSPPILPTTSNTLTLYVTT 85
           I  I +L S+I F+     L   +YLK++ S+LSSS   S+ +P    + S+T    +  
Sbjct: 53  ILFIIVLLSVIFFICSILHLLVRYYLKKKRSNLSSSPNESNQNPEF--SDSDTYQRQLQQ 112

Query: 86  ACQV-DLKVDNP-IDKLPKIQFNEELKTRDSL-CCVCLGEFEIKEELLQVPSCKHVFHID 145
              + D  +D   ID LP   + E   T++   C VCL EF   ++L  +P+C H FHID
Sbjct: 113 LFHLHDSGLDQALIDALPVFLYKEIKGTKEPFDCAVCLCEFSEDDKLRLLPNCSHAFHID 172

Query: 146 CMNHWLISNSTCPLCRCSVIS 156
           C++ WL+SNSTCPLCR ++ S
Sbjct: 173 CIDTWLLSNSTCPLCRGTLFS 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84TF51.4e-4967.92Probable E3 ubiquitin-protein ligase RHA4A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q570X51.3e-2343.07RING-H2 finger protein ATL58 OS=Arabidopsis thaliana OX=3702 GN=ATL58 PE=2 SV=1[more]
Q9SN282.1e-2138.46RING-H2 finger protein ATL7 OS=Arabidopsis thaliana OX=3702 GN=ATL7 PE=2 SV=1[more]
Q9SN271.9e-1436.64E3 ubiquitin-protein ligase ATL59 OS=Arabidopsis thaliana OX=3702 GN=ATL59 PE=1 ... [more]
Q8GW383.1e-1238.30RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EM646.5e-9889.72probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1I2V61.2e-9688.79probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1H2A42.8e-9385.84probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1DWP02.4e-9286.67probable E3 ubiquitin-protein ligase RHA4A OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1K5R33.1e-9285.78probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038907033.11.3e-10093.40probable E3 ubiquitin-protein ligase RHA4A [Benincasa hispida][more]
XP_022928899.11.3e-9789.72probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata][more]
XP_022970098.12.5e-9688.79probable E3 ubiquitin-protein ligase RHA4A [Cucurbita maxima][more]
XP_023550207.19.6e-9686.70probable E3 ubiquitin-protein ligase RHA4A isoform X1 [Cucurbita pepo subsp. pep... [more]
KAG6579565.11.3e-9589.5730S ribosomal protein S6 alpha, chloroplastic, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
AT4G24015.11.0e-5067.92RING/U-box superfamily protein [more]
AT1G33480.19.5e-2543.07RING/U-box superfamily protein [more]
AT4G10150.11.5e-2238.46RING/U-box superfamily protein [more]
AT4G10160.11.4e-1536.64RING/U-box superfamily protein [more]
AT1G23980.12.2e-1338.30RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 108..149
e-value: 0.002
score: 27.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 108..150
e-value: 1.8E-11
score: 44.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 108..150
score: 11.766381
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 76..155
e-value: 9.9E-17
score: 62.4
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 4..164
NoneNo IPR availablePANTHERPTHR22765:SF187E3 UBIQUITIN-PROTEIN LIGASE RHA4A-RELATEDcoord: 4..164
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 88..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G001630.1Lsi09G001630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity