Lsi09G001480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G001480
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationchr09: 1497210 .. 1500893 (-)
RNA-Seq ExpressionLsi09G001480
SyntenyLsi09G001480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCTAAACGGACGGTGGCGGAGCTAAATTCAGTGTTTTCCATTTGTACAGTTCAATTCAATCTGGACAGAAAGAACACAACGACGACTTCAACAATTCAACGAATCCTATTCCTTTTCTGTGTTTCCCTTTTCTTTTCATTCGCTTAATTTTAGCTCTCAAATCTCTGTTCCTGTTTAGGGTTTCCCTTTCGATTTGGGTTAAGGAGTTATGCGGATGCGGATGCTTAAATGGCCGAACCTCCGTTTCGATTCATAATCAGATTCACCGGGAACTTTGTTCCTCCAATTTCTACTTACCCATTTCAATTCGAGATGAAATTGTTCATGGGTCGTCGATTTCACTTTCACTCTGCCTTCGATGTGGCTTCCGAGGCCTTTTCTGAACACCTTTCTCCTTTAATCGTCGTTATCTGACTGTTTGCTGTTTTCAATTACTGGCTTTTATCTCTCTTTTCTTATATCTTTTTCGAGATTCCTCTTTCTTTTTTCTTTCGTCTTTCTGCGTTGCGATGAGTTGCGGTTCGGATTTGATGATGTCTGGGAATTCTGAGGCTGATGATCGTGAGATTGTTGAAAAGGATCCATCAGGACGGTACATACGGGTATTTCTTTTGAATTCTCTTCTTAGGTTTTGGATATTTTGATTACTTGTTTCTCCTCAGCTGAATTGCCCTAGCTCTTAAGATCAGATAGAGGAATTCTTTTCCATATTGTTCCCTTTAACTGTTTTGGGCTGGTGTTTGGTTATTCAATTTTTAAATGTGGATGATCTTAATTTAGGGATTCAGTCGTGCTGATAGTGGATTGAATTGGGTTGTTTGGCTTTTGTAAGGGGAGAATTTGGTTGTTTTCTTTTATTGCTAATGGTTCAATCATCGATTACTTGTTTTAGATTTGTTCTTTCCCTTTTGTTTTATGGTTTTGATGTTTTGTTATTTGACTTAATCCTCATTTGAACTTTTTTTTCCTTTTTTTTTTCAGTATGATGAGATTTTGGGTAAAGGGGCATTCAAGACAGTGTATGCTTCTGCTGTCTATCAATCTTTTTCCTTTTATTGCCTTTTCTTTTCTAGTTTGTTCATTAGTTTATGTTGAGTTTTAGGGTATAATGAGATTTTAGGGTGTATTATTTGACTTGAGGTCTGTTTATGTTGTCATTAGATACAAAGCATTCGACGAAGTTGGCGGAATCGAAGTTGCTTGGAGTCAAGTGGACATTGAAGATGTTTTGCAGTCACCAGAACAACTTCAAAGATTGTATTCTGAGGTCCATCTGTTGAAGTCACTGAAACATGAAAATATCATCAAGTTCTATAGCTATTGGGTTGATGATAAACAAAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAGTAAGTTCCAAATAATCTTCTTTTTGTTTTCCATTATTTGTTGATCGTTATCTTTTTGCTACATTCATTGAGTTACTTATTCATTTATGGTATACTTTGGAATTCATCAGATATCGAAAGAAGCATAGGAAAGTTGACTTGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGAGGTTTAACATATTTGCATGGTCACAACCCTCCTATCATTCACAGGGATTTGAAATGTGATAATATATTTGTTAATGGGAATACTGGAGAAGTTAAAATTGGAGATCTAGGATTGGCGATTGTGATGCAGCAGCCTACTGCTCGAAGTGTAATTGGTAATAGTTGTTTTTTTAGCTTGATTTTTTTTTGTTGTGTGGATAGAATTCTAGATTGAAAGAGAACAATCTTCAACTGATATCTGGGCATGTGATTATTATTCAGGCACTCCAGAGTTCATGGCTCCAGAACTTTATGATGAGGACTATAATGAACTTGTTGACATATATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCATATAATGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCGGTAAGTAAATGATTTACATCTACCAAATTTTGTTCTCATGTTTCCATCAAATGAAAGTCCTGAAATCATTTATTCTTTCTTGCAGGGTATAAAGCCTGCTTCCCTTGAGAAAGTGCTGGACCCTCAAGTTAAGCAATTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCTTTCCTTGCAACTGAAAGTCCCAAGGAGCATAGTTCTGAACTTTCAAGGTTGTTGAATGAGCAGATCAAATCTGTGAATCCTCCTCTGCCTGGTTCTCATCCAATGGACACAGACCCTAACAGCAGCAAACTCTCAGCCTCAGTGGGCTCATCTGTCAAAAGCAATAATGGAATTTCTCACTTTACAACTCTAGAACTTCAGAGGCTCTCTGAGAATAATGAATTGACACTGAAAGGGGACATGACTGATCATAATACAATGTCATTCCATTTGCGTATCGCTGAACTATATGGTAACTTTCGGTCAATTCTTCCGTAATCTTCTTGAAGTTGTTTTTATTCTTTGGCCGAATGTGTTGCAATGACTTGAACATTTATTTTCATGTAGGTAAATCTAGGAATATCCATTTTGCCTTCTATCTCGACTCTGATACTTCTATGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTATCATAGCAAAACTAATTGATGAATTAATTGCCAAGTTTGTTCCTAGCTGGAAACCATGTCCAAACTATTGTCAAGAACAAAATACTTCACATAGTTCTTCTGAAACTCAAGAGGATAAGACCTTCATTCCTCCTTTCTTCTCTGAGCTAGTACTGTCATCTCCCATGGTGGCTGCTGCTGCAAGGAATAACCCGACGGATTTGACCAAGGTCGAAGATCAAGAAAACCAGCAGTCGATTATTTCGTGTGCTTCCGTGGAATACATTTACTCAACTGTCTCAGATTACAGCATAGGAAAAGGATCAGAATGTGGTGAGTTTGGCCACCCTGAATGCGACAAAGCTTATATTAGCAATGGAACAATCGATTTAGATTCAGAAGCTGTTGGATCTATTCCAACAATGATCGATTTTGCAAAACCATCTCTAATCTCCTCCTGCTCTGGAATGTCCAAAGAATTGAGTTTATCTAGCTTTTCCACTCTTTCTCTGGATGAAAGAGATCACCAAGATGAACTCAAAATGGAGATTGATGCTATTGATTTGCAGTACCACCAATGCCTGTGTGAACTGTCAAGAATGAGAGAGGAAGCAATAGAAACTGCCAAGAAGAGGTGGATGTCAAAGAAGAAAGCTACTGGTATTTGAAGCCTTCCTTTATACTTTGGATTTATAAGTTCCCTTTTTCTTTATTTTCTTGGCAAACTCTTGCTTGCAAAATGGTCATCTTTTTGTTTTTTGTTTTTTGGTTTTTAAAAACTACTGACTGGTATTCTTTGCATTCTATCCAACCAAGTCCTAACTCCTAACTTTTGACTCTGAACATTTTTAATTTATACCAATTCATTTCATAGTTTAGACATAAATTGCTACATTTGTTCCACATTCTTCCCAGAATGCAATTTTAGAGTTCAATATTTTCCTTGTATTTTTCTCACTGTCATGCAATTTTCCCTTTTTTATGATATTCAATAAATATGATATCATCTTCCATTTGTGGCAA

mRNA sequence

TGCTAAACGGACGGTGGCGGAGCTAAATTCAGTGTTTTCCATTTGTACAGTTCAATTCAATCTGGACAGAAAGAACACAACGACGACTTCAACAATTCAACGAATCCTATTCCTTTTCTGTGTTTCCCTTTTCTTTTCATTCGCTTAATTTTAGCTCTCAAATCTCTGTTCCTGTTTAGGGTTTCCCTTTCGATTTGGGTTAAGGAGTTATGCGGATGCGGATGCTTAAATGGCCGAACCTCCGTTTCGATTCATAATCAGATTCACCGGGAACTTTGTTCCTCCAATTTCTACTTACCCATTTCAATTCGAGATGAAATTGTTCATGGGTCGTCGATTTCACTTTCACTCTGCCTTCGATGTGGCTTCCGAGGCCTTTTCTGAACACCTTTCTCCTTTAATCGTCGTTATCTGACTGTTTGCTGTTTTCAATTACTGGCTTTTATCTCTCTTTTCTTATATCTTTTTCGAGATTCCTCTTTCTTTTTTCTTTCGTCTTTCTGCGTTGCGATGAGTTGCGGTTCGGATTTGATGATGTCTGGGAATTCTGAGGCTGATGATCGTGAGATTGTTGAAAAGGATCCATCAGGACGGTACATACGGGGTGTATTATTTGACTTGAGGTCTGTTTATGTTGTCATTAGATACAAAGCATTCGACGAAGTTGGCGGAATCGAAGTTGCTTGGAGTCAAGTGGACATTGAAGATGTTTTGCAGTCACCAGAACAACTTCAAAGATTGTATTCTGAGGTCCATCTGTTGAAGTCACTGAAACATGAAAATATCATCAAGTTCTATAGCTATTGGGTTGATGATAAACAAAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAAGAAGCATAGGAAAGTTGACTTGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGAGGTTTAACATATTTGCATGGTCACAACCCTCCTATCATTCACAGGGATTTGAAATGTGATAATATATTTGTTAATGGGAATACTGGAGAAGTTAAAATTGGAGATCTAGGATTGGCGATTGTGATGCAGCAGCCTACTGCTCGAAGTGTAATTGGCACTCCAGAGTTCATGGCTCCAGAACTTTATGATGAGGACTATAATGAACTTGTTGACATATATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCATATAATGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCGGGTATAAAGCCTGCTTCCCTTGAGAAAGTGCTGGACCCTCAAGTTAAGCAATTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCTTTCCTTGCAACTGAAAGTCCCAAGGAGCATAGTTCTGAACTTTCAAGGTTGTTGAATGAGCAGATCAAATCTGTGAATCCTCCTCTGCCTGGTTCTCATCCAATGGACACAGACCCTAACAGCAGCAAACTCTCAGCCTCAGTGGGCTCATCTGTCAAAAGCAATAATGGAATTTCTCACTTTACAACTCTAGAACTTCAGAGGCTCTCTGAGAATAATGAATTGACACTGAAAGGGGACATGACTGATCATAATACAATGTCATTCCATTTGCGTATCGCTGAACTATATGGTAAATCTAGGAATATCCATTTTGCCTTCTATCTCGACTCTGATACTTCTATGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTATCATAGCAAAACTAATTGATGAATTAATTGCCAAGTTTGTTCCTAGCTGGAAACCATGTCCAAACTATTGTCAAGAACAAAATACTTCACATAGTTCTTCTGAAACTCAAGAGGATAAGACCTTCATTCCTCCTTTCTTCTCTGAGCTAGTACTGTCATCTCCCATGGTGGCTGCTGCTGCAAGGAATAACCCGACGGATTTGACCAAGGTCGAAGATCAAGAAAACCAGCAGTCGATTATTTCGTGTGCTTCCGTGGAATACATTTACTCAACTGTCTCAGATTACAGCATAGGAAAAGGATCAGAATGTGGTGAGTTTGGCCACCCTGAATGCGACAAAGCTTATATTAGCAATGGAACAATCGATTTAGATTCAGAAGCTGTTGGATCTATTCCAACAATGATCGATTTTGCAAAACCATCTCTAATCTCCTCCTGCTCTGGAATGTCCAAAGAATTGAGTTTATCTAGCTTTTCCACTCTTTCTCTGGATGAAAGAGATCACCAAGATGAACTCAAAATGGAGATTGATGCTATTGATTTGCAGTACCACCAATGCCTGTGTGAACTGTCAAGAATGAGAGAGGAAGCAATAGAAACTGCCAAGAAGAGGTGGATGTCAAAGAAGAAAGCTACTGGTATTTGAAGCCTTCCTTTATACTTTGGATTTATAAGTTCCCTTTTTCTTTATTTTCTTGGCAAACTCTTGCTTGCAAAATGGTCATCTTTTTGTTTTTTGTTTTTTGGTTTTTAAAAACTACTGACTGGTATTCTTTGCATTCTATCCAACCAAGTCCTAACTCCTAACTTTTGACTCTGAACATTTTTAATTTATACCAATTCATTTCATAGTTTAGACATAAATTGCTACATTTGTTCCACATTCTTCCCAGAATGCAATTTTAGAGTTCAATATTTTCCTTGTATTTTTCTCACTGTCATGCAATTTTCCCTTTTTTATGATATTCAATAAATATGATATCATCTTCCATTTGTGGCAA

Coding sequence (CDS)

ATGAGTTGCGGTTCGGATTTGATGATGTCTGGGAATTCTGAGGCTGATGATCGTGAGATTGTTGAAAAGGATCCATCAGGACGGTACATACGGGGTGTATTATTTGACTTGAGGTCTGTTTATGTTGTCATTAGATACAAAGCATTCGACGAAGTTGGCGGAATCGAAGTTGCTTGGAGTCAAGTGGACATTGAAGATGTTTTGCAGTCACCAGAACAACTTCAAAGATTGTATTCTGAGGTCCATCTGTTGAAGTCACTGAAACATGAAAATATCATCAAGTTCTATAGCTATTGGGTTGATGATAAACAAAAGACTATCAACATGATTACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAAGAAGCATAGGAAAGTTGACTTGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGAGGTTTAACATATTTGCATGGTCACAACCCTCCTATCATTCACAGGGATTTGAAATGTGATAATATATTTGTTAATGGGAATACTGGAGAAGTTAAAATTGGAGATCTAGGATTGGCGATTGTGATGCAGCAGCCTACTGCTCGAAGTGTAATTGGCACTCCAGAGTTCATGGCTCCAGAACTTTATGATGAGGACTATAATGAACTTGTTGACATATATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCATATAATGAGTGCAAAAATTCTGCCCAAATATTCAAGAAGGTTACTTCGGGTATAAAGCCTGCTTCCCTTGAGAAAGTGCTGGACCCTCAAGTTAAGCAATTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCTTTCCTTGCAACTGAAAGTCCCAAGGAGCATAGTTCTGAACTTTCAAGGTTGTTGAATGAGCAGATCAAATCTGTGAATCCTCCTCTGCCTGGTTCTCATCCAATGGACACAGACCCTAACAGCAGCAAACTCTCAGCCTCAGTGGGCTCATCTGTCAAAAGCAATAATGGAATTTCTCACTTTACAACTCTAGAACTTCAGAGGCTCTCTGAGAATAATGAATTGACACTGAAAGGGGACATGACTGATCATAATACAATGTCATTCCATTTGCGTATCGCTGAACTATATGGTAAATCTAGGAATATCCATTTTGCCTTCTATCTCGACTCTGATACTTCTATGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTATCATAGCAAAACTAATTGATGAATTAATTGCCAAGTTTGTTCCTAGCTGGAAACCATGTCCAAACTATTGTCAAGAACAAAATACTTCACATAGTTCTTCTGAAACTCAAGAGGATAAGACCTTCATTCCTCCTTTCTTCTCTGAGCTAGTACTGTCATCTCCCATGGTGGCTGCTGCTGCAAGGAATAACCCGACGGATTTGACCAAGGTCGAAGATCAAGAAAACCAGCAGTCGATTATTTCGTGTGCTTCCGTGGAATACATTTACTCAACTGTCTCAGATTACAGCATAGGAAAAGGATCAGAATGTGGTGAGTTTGGCCACCCTGAATGCGACAAAGCTTATATTAGCAATGGAACAATCGATTTAGATTCAGAAGCTGTTGGATCTATTCCAACAATGATCGATTTTGCAAAACCATCTCTAATCTCCTCCTGCTCTGGAATGTCCAAAGAATTGAGTTTATCTAGCTTTTCCACTCTTTCTCTGGATGAAAGAGATCACCAAGATGAACTCAAAATGGAGATTGATGCTATTGATTTGCAGTACCACCAATGCCTGTGTGAACTGTCAAGAATGAGAGAGGAAGCAATAGAAACTGCCAAGAAGAGGTGGATGTCAAAGAAGAAAGCTACTGGTATTTGA

Protein sequence

MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAAARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNGTIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI
Homology
BLAST of Lsi09G001480 vs. ExPASy Swiss-Prot
Match: Q944Q0 (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=1 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 4.9e-154
Identity = 320/618 (51.78%), Postives = 399/618 (64.56%), Query Frame = 0

Query: 13  SEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPE 72
           S  ++ +  EKDPSGRYIR      R  +  + YKAFDEV GIEVAW+ V IEDV+Q P 
Sbjct: 12  SSMEEADFAEKDPSGRYIRYDDVLGRGAFKTV-YKAFDEVDGIEVAWNLVSIEDVMQMPG 71

Query: 73  QLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLK 132
           QL+RLYSEVHLLK+LKHENIIK +  WVD+K KTINMITELFTSGSLR YRKKHRKVD K
Sbjct: 72  QLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPK 131

Query: 133 AFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARS 192
           A KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTGEVKIGDLGLA V+QQPTARS
Sbjct: 132 AIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARS 191

Query: 193 VIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPA 252
           VIGTPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPYNEC+N AQI+KKVTS IKP 
Sbjct: 192 VIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQ 251

Query: 253 SLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQIKSVN 312
           SL KV DPQV+QFIEKCL+PAS R  A EL KDPFLA +  K+  S L    +   K V 
Sbjct: 252 SLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKD--SALLASSSTSSKYVR 311

Query: 313 PPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNELTLKGDMTDHNT 372
           PP     PMD D N +K       SV SN       T+ELQR++EN E  L+G+ +D  T
Sbjct: 312 PPQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVT 371

Query: 373 MSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIAKF 432
            S  LRIA+  GK R +HFAFYL+SDT+ AIA EMVE+L L++++  +IA +ID+ I + 
Sbjct: 372 ASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQL 431

Query: 433 VPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAAARNNPTDLTKVED 492
           +       +     +  +S   T ED                     A N  T  +K E+
Sbjct: 432 L------SDRTSSHHNQNSPRLTHEDH-------------------EAANQQTVNSKDEE 491

Query: 493 QENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNGTIDLDSEAVGSIP 552
               QS+ S  S +Y +     YS   G+   E G                D+E++    
Sbjct: 492 AAG-QSMKSDISADYYF----PYSANDGNAAMEAGR---------------DAESMS--- 551

Query: 553 TMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAIDLQYHQCLCELSR 612
                   S + SCS M      S+   LS+ + D+ ++LK E++ I+ Q++Q   +L +
Sbjct: 552 --------SYLDSCSMM------STIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLK 557

Query: 613 MREEAIETAKKRWMSKKK 631
           ++E+AIE AK++W++KK+
Sbjct: 612 LKEDAIENAKRKWITKKQ 557

BLAST of Lsi09G001480 vs. ExPASy Swiss-Prot
Match: Q6EU49 (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK4 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 2.2e-146
Identity = 316/633 (49.92%), Postives = 406/633 (64.14%), Query Frame = 0

Query: 14  EADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 73
           + DD E VE DP+ RYIR      R     + YKAFDEV GIEVAWSQV+I++V+QSP+ 
Sbjct: 9   KVDDAECVEVDPTRRYIRYNEVLGRGAMKTV-YKAFDEVEGIEVAWSQVEIDEVMQSPDN 68

Query: 74  LQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKA 133
           L+RLYSEVHLLKSLKHEN++KFY+YWVDD++KTIN+ITELFTSGSLRQYR+KH +VDLKA
Sbjct: 69  LERLYSEVHLLKSLKHENVMKFYNYWVDDQKKTINVITELFTSGSLRQYRQKHPRVDLKA 128

Query: 134 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSV 193
            KNWARQ+LRGL YLH H PPIIHRDLKCDNIFVNGN GEVKIGDLGLA VM  P A+SV
Sbjct: 129 IKNWARQVLRGLDYLHTHQPPIIHRDLKCDNIFVNGNHGEVKIGDLGLATVMLTPRAKSV 188

Query: 194 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPAS 253
           IGTPEFMAPELYDE+Y+ELVDIYSFGMCMLE+ T EYPY+EC N+AQIFKKV+ G+KPA+
Sbjct: 189 IGTPEFMAPELYDENYDELVDIYSFGMCMLEMFTLEYPYSECTNAAQIFKKVSKGVKPAA 248

Query: 254 LEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL--SRLLNEQIKSV 313
           L K+ + Q KQFI+KCLVPAS RL A ELL+DPFL +    ++SS L  ++  +   KSV
Sbjct: 249 LAKITNIQAKQFIDKCLVPASERLSAKELLQDPFLCS----DNSSVLVGTKFPSSLPKSV 308

Query: 314 NPPLPGSHPMDTDPNSSKLSASVGSSVKSNN-GISHFTTLELQRLSENNELTLKGDMTDH 373
           +  L   H MD D N S  +    S+ K N+ G  H + LE  R ++N EL L G+  D 
Sbjct: 309 DVSLEALH-MDVDTNESMCT----STCKRNDLGGPHRSVLEFTRTNKNTELKLTGEKLDD 368

Query: 374 NTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIA 433
           N++S  LRIA+L G +RNIHF FYLDSDT+M++A EMVEQLEL++ D T IA  ID LI 
Sbjct: 369 NSVSLVLRIADLCGHARNIHFLFYLDSDTAMSVAAEMVEQLELADCDVTFIADFIDLLIV 428

Query: 434 KFVPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSEL-----VLSSPMVAAAARNNPT 493
             VP  +   N      +S S     E         SEL     ++   M +  A  +P+
Sbjct: 429 NLVPG-QQLMNDAVMSTSSESKMGESEHVITSQQHPSELTHDYVLVEGMMHSKEANASPS 488

Query: 494 D-------LTKVEDQENQQSIISCASVEYIYSTVSDYSIG--KGSECGEFGHPECDKAYI 553
           D        T +    + +       ++    ++S+Y +   +  ECG +          
Sbjct: 489 DYIDSLLNATNLGGPNSSEGSDISVQLDGSSKSLSEYGVDEYRTLECGAY---------- 548

Query: 554 SNGTIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEI 613
             GT     + +G    + + +    I      S    L   +++S+ E  ++D L  E+
Sbjct: 549 -KGT-----DKLGCRHPLSNGSSNFAIFQMDQASHHSELVIGASVSITE--NRDVLNGEL 608

Query: 614 DAIDLQYHQCLCELSRMREEAIETAKKRWMSKK 630
             I+ QY Q   EL+RMREEA+E A+K+W+  K
Sbjct: 609 GLIEAQYEQWFRELTRMREEALEGARKKWLPDK 612

BLAST of Lsi09G001480 vs. ExPASy Swiss-Prot
Match: Q8RXE5 (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 GN=WNK10 PE=2 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 6.2e-141
Identity = 300/611 (49.10%), Postives = 372/611 (60.88%), Query Frame = 0

Query: 16  DDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQ 75
           ++ + V+KDP+GRYIR      R  +  + YKAFDEV GIEVAW+ + IEDVLQ P QL 
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTV-YKAFDEVEGIEVAWNLMSIEDVLQMPGQLD 61

Query: 76  RLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKAFK 135
           RLYSEVHLL SLKH+NIIK +  WVDD  K+INMITELFTSGSL  YRKKHRKVD KA  
Sbjct: 62  RLYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIM 121

Query: 136 NWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIG 195
           NWARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG+VKIGDLGLA VMQQPTARSVIG
Sbjct: 122 NWARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIG 181

Query: 196 TPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLE 255
           TPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSGIKP SL 
Sbjct: 182 TPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLS 241

Query: 256 KVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQIKSVNPPL 315
           KV DPQVKQFIEKCL+PA  R  A ELLKD  LA +  K+  S L+   N   K   PP 
Sbjct: 242 KVDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKD--STLTASSNTTFKPAMPPQ 301

Query: 316 PGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNELTLKGDMTDHNTMSF 375
               PMD +      S S+ SS KS+   +   T+E+QR++E+ E  L G+  D    S 
Sbjct: 302 CEYRPMDVE-YKKNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASM 361

Query: 376 HLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIAKFVPS 435
            LRIA   G++R + F F L +DT+ A+  EMVE+L+LS+ + T+IA++IDELI K    
Sbjct: 362 ALRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMK---- 421

Query: 436 WKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAAARNNPTDLTKVEDQEN 495
                                                  + A  +  N   + + +D+E 
Sbjct: 422 ---------------------------------------LKANRSLPNANSVYQSKDEEA 481

Query: 496 QQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNGTIDLDSEAVGSIPTMI 555
            +S+ S  S +Y +   S+     G  C                      EAV S+ +  
Sbjct: 482 GESMKSEISADYYHRVSSNEGSRLGCCC----------------------EAVESLLS-- 521

Query: 556 DFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAIDLQYHQCLCELSRMRE 615
                S + SCS +S + S               ++LK E++ I+ QY+Q    L RM+E
Sbjct: 542 -----SFLDSCSMVSNKQS---------------EDLKTELNVIESQYNQSCQRLLRMKE 521

Query: 616 EAIETAKKRWM 627
           EAIE AK++WM
Sbjct: 602 EAIEKAKRKWM 521

BLAST of Lsi09G001480 vs. ExPASy Swiss-Prot
Match: Q8S8Y8 (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN=WNK6 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 8.7e-135
Identity = 257/442 (58.14%), Postives = 312/442 (70.59%), Query Frame = 0

Query: 10  SGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQ 69
           S   E  D E++E DP+ RYIR      +  +  + YKAFDEV GIEVAW+QV I+DVLQ
Sbjct: 8   SALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTV-YKAFDEVDGIEVAWNQVRIDDVLQ 67

Query: 70  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKV 129
           SP  L+RLYSEV LLKSLKH NII+FY+ W+DDK KT+N+ITELFTSGSLR YRKKHRKV
Sbjct: 68  SPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKV 127

Query: 130 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 189
           ++KA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q  
Sbjct: 128 NMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQAN 187

Query: 190 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 249
           A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV+SGI
Sbjct: 188 AKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGI 247

Query: 250 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES--------------PKE 309
           KPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL                  PKE
Sbjct: 248 KPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKE 307

Query: 310 HSSELSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRL 369
            +     L++E   +  P    S  +D D N   ++ S  S  +          +E++R 
Sbjct: 308 GAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSR---------CIEVRRA 367

Query: 370 SENNELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSN 429
              N   LKG+  D  ++S  LRI +  G+ RNIHF FY + DT+  ++ EMVEQLEL++
Sbjct: 368 KRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTD 427

Query: 430 EDATIIAKLIDELIAKFVPSWK 438
           ++ T IA+LID L+   +P+WK
Sbjct: 428 QNVTFIAELIDILLVNMIPTWK 439

BLAST of Lsi09G001480 vs. ExPASy Swiss-Prot
Match: Q8LST2 (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN=WNK7 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 2.7e-128
Identity = 282/630 (44.76%), Postives = 367/630 (58.25%), Query Frame = 0

Query: 14  EADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 73
           E  D E++E DP+ RYIR      +     + +K FDEV GIEVAW+QV I+D+LQSP+ 
Sbjct: 12  EPPDPEVLEIDPTCRYIRYKEVIGKGASKTV-FKGFDEVDGIEVAWNQVRIDDLLQSPDC 71

Query: 74  LQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKA 133
           L+RLYSEV LLKSLKH+NII+FY+ W+DDK KT+N+ITELFTSGSLRQYRKKHRKV++KA
Sbjct: 72  LERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKKHRKVNMKA 131

Query: 134 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSV 193
            K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN GEVKIGDLGLA VM+Q  A+SV
Sbjct: 132 VKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVMEQANAKSV 191

Query: 194 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPAS 253
           IGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT EYPY EC+NSAQI+KKV+SGIKPAS
Sbjct: 192 IGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKVSSGIKPAS 251

Query: 254 LEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES--------------PKEHSSE 313
           L KV DP+V +FIEKCL+PAS RL A ELL D FL                  PKE S  
Sbjct: 252 LSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIVMPKEGSFG 311

Query: 314 LSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENN 373
              L++E      PP   +  M  + +      ++   + SNN  S    +E++R    N
Sbjct: 312 ERCLMSE-----GPPNARNRTMSMNLDEDN---NLPIVISSNN--SGTNCIEVRRAKRGN 371

Query: 374 ELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDAT 433
              LKG+  D N++S  LRI +  G+ RNIHF F+ + DT+  ++ EMVEQLEL++++  
Sbjct: 372 FFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVK 431

Query: 434 IIAKLIDELIAKFVPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAA 493
            IA+LID L+   +P+WK               ++   D    P                
Sbjct: 432 FIAELIDVLLVNLIPNWK---------------TDVAVDHLIHP---------------- 491

Query: 494 ARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNG 553
                        Q+NQ             S+  ++  G  S+ GE         Y    
Sbjct: 492 -------------QQNQ-------------SSKDNHQNGASSQAGESISHSLSSDY---- 551

Query: 554 TIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAI 613
                       P   D A P++ ++      E         SL+E +  + LK E++ I
Sbjct: 552 -----------CPRSDDEANPTVAATTEDQEAE------KPGSLEEEEEDERLKEELEKI 552

Query: 614 DLQYHQCLCELSRMREEAIETAKKRWMSKK 630
           + ++ + + E++R REEA    K R+  KK
Sbjct: 612 EERFREEMKEITRKREEATMETKNRFFEKK 552

BLAST of Lsi09G001480 vs. ExPASy TrEMBL
Match: A0A5A7TLP6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001600 PE=4 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 580/637 (91.05%), Postives = 599/637 (94.03%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           MSCGSDL+MS NSEADDREIVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 1   MSCGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 60

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLR
Sbjct: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLR 120

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  ESPKEHSSEL
Sbjct: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSEL 300

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SR LNEQ KSVNPPL GSHPMDTD   +KLS SV SSVKSNNGISHF+T ELQRL+ENNE
Sbjct: 301 SRSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNE 360

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKG+MTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTS+AIALEMVEQLELSNEDATI
Sbjct: 361 LTLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATI 420

Query: 421 IAKLIDELIAKFVPSWKPCPNYCQE--QNTSHSSSETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVPSWKPCPNYC+E  QNT  S SE QE+K+FI PFFSELVLSSPMV  
Sbjct: 421 IAKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMV-V 480

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           AARNN T L KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHP+C+KAYIS+
Sbjct: 481 AARNNLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 540

Query: 541 GTIDLDSEAVGS-IPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEID 600
           GTIDLD+EAVGS I TMIDFAKPSLISSCSGMSKELSLSSFSTLS++ERDHQDELKMEID
Sbjct: 541 GTIDLDAEAVGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEID 600

Query: 601 AIDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           AIDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 601 AIDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Lsi09G001480 vs. ExPASy TrEMBL
Match: A0A1S3AUL5 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103482852 PE=4 SV=1)

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 579/637 (90.89%), Postives = 598/637 (93.88%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           MSCGSDL+MS NSEADDREIVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 1   MSCGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 60

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLR
Sbjct: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLR 120

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  ESPKEHSSEL
Sbjct: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSEL 300

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SR LNEQ KSVNPPL GSHPMDTD   +KLS SV SSVKSNNGISHF+T ELQRL+ENNE
Sbjct: 301 SRSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNE 360

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKG+MTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTS+AIALEMVEQLELSNEDATI
Sbjct: 361 LTLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATI 420

Query: 421 IAKLIDELIAKFVPSWKPCPNYCQE--QNTSHSSSETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVPSWKPCPNYC+E  QNT  S SE QE+K+FI PFFSELVLSSPMV  
Sbjct: 421 IAKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMV-V 480

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           AARNN T L KVEDQENQQSIISCAS EYIYSTVSDYSIGKGSECGEFGHP+C+KAYIS+
Sbjct: 481 AARNNLTGLAKVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 540

Query: 541 GTIDLDSEAVGS-IPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEID 600
           GTIDLD+EAVGS I TMIDFAKPSLISSCSGMSKELSLSSFSTLS++ERDHQDELKMEID
Sbjct: 541 GTIDLDAEAVGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEID 600

Query: 601 AIDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           AIDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 601 AIDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Lsi09G001480 vs. ExPASy TrEMBL
Match: A0A0A0KQP1 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5G148620 PE=4 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 577/636 (90.72%), Postives = 598/636 (94.03%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           +SCGSDL+MSGNSEADDR IVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 47  LSCGSDLIMSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 106

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLR
Sbjct: 107 QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLR 166

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 167 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 226

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 227 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 286

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPAS RLPASELLKDPFLA ESPK++SSEL
Sbjct: 287 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSEL 346

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SR LNE  KSVNPPL GSHPM+TD N +KLS SV SSVKSNNGISHF+T ELQRL+ENNE
Sbjct: 347 SRSLNEHFKSVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNE 406

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTS+AIALEMVEQLELSNEDATI
Sbjct: 407 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATI 466

Query: 421 IAKLIDELIAKFVPSWKPCPNYC--QEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVPSWKPCPNYC  Q+QNT H S E QEDKTFI PFFSELVLSSPMV A
Sbjct: 467 IAKLIDELIAKFVPSWKPCPNYCEEQQQNTPH-SPEAQEDKTFISPFFSELVLSSPMV-A 526

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           AARNN T L KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHP+C+KAYIS+
Sbjct: 527 AARNNLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 586

Query: 541 GTIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDA 600
           GTIDLD+EAVGS+ T IDFAKPSLISSCS MSKELSLSSFSTLS++ERDHQDELKMEIDA
Sbjct: 587 GTIDLDAEAVGSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDA 646

Query: 601 IDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           IDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 647 IDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 679

BLAST of Lsi09G001480 vs. ExPASy TrEMBL
Match: A0A1S4DS99 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103482852 PE=4 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 552/603 (91.54%), Postives = 571/603 (94.69%), Query Frame = 0

Query: 37  LRSVYV--VIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIK 96
           LR +++  +  YKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIK
Sbjct: 6   LRRIHLDDIYGYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIK 65

Query: 97  FYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPP 156
           FYSYWVDDK KTINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPP
Sbjct: 66  FYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPP 125

Query: 157 IIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVD 216
           IIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVD
Sbjct: 126 IIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVD 185

Query: 217 IYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPAS 276
           IYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPAS
Sbjct: 186 IYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPAS 245

Query: 277 MRLPASELLKDPFLATESPKEHSSELSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASV 336
           MRLPASELLKDPFL  ESPKEHSSELSR LNEQ KSVNPPL GSHPMDTD   +KLS SV
Sbjct: 246 MRLPASELLKDPFLVAESPKEHSSELSRSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSV 305

Query: 337 GSSVKSNNGISHFTTLELQRLSENNELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFY 396
            SSVKSNNGISHF+T ELQRL+ENNELTLKG+MTDHNTMSFHLRIAELYGKSRNIHFAFY
Sbjct: 306 ASSVKSNNGISHFSTQELQRLTENNELTLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFY 365

Query: 397 LDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYCQE--QNTSHSS 456
           LDSDTS+AIALEMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYC+E  QNT  S 
Sbjct: 366 LDSDTSLAIALEMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSP 425

Query: 457 SETQEDKTFIPPFFSELVLSSPMVAAAARNNPTDLTKVEDQENQQSIISCASVEYIYSTV 516
           SE QE+K+FI PFFSELVLSSPMV  AARNN T L KVEDQENQQSIISCAS EYIYSTV
Sbjct: 426 SEAQEEKSFISPFFSELVLSSPMV-VAARNNLTGLAKVEDQENQQSIISCASAEYIYSTV 485

Query: 517 SDYSIGKGSECGEFGHPECDKAYISNGTIDLDSEAVGS-IPTMIDFAKPSLISSCSGMSK 576
           SDYSIGKGSECGEFGHP+C+KAYIS+GTIDLD+EAVGS I TMIDFAKPSLISSCSGMSK
Sbjct: 486 SDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAVGSTISTMIDFAKPSLISSCSGMSK 545

Query: 577 ELSLSSFSTLSLDERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIETAKKRWMSKKKA 635
           ELSLSSFSTLS++ERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIE+AKKRWMSKKKA
Sbjct: 546 ELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAKKRWMSKKKA 605

BLAST of Lsi09G001480 vs. ExPASy TrEMBL
Match: A0A6J1EQ95 (serine/threonine-protein kinase WNK8-like OS=Cucurbita moschata OX=3662 GN=LOC111435638 PE=4 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 1.1e-289
Identity = 527/633 (83.25%), Postives = 559/633 (88.31%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           MSCGSDL MSGNSEADDREIVEKDP+GRYIR      +  +  + Y+AFDEVGGIEVAWS
Sbjct: 1   MSCGSDL-MSGNSEADDREIVEKDPTGRYIRYDEILGKGAFKTV-YRAFDEVGGIEVAWS 60

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFY YWVDDK KTINMITELFTSGSLR
Sbjct: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYCYWVDDKNKTINMITELFTSGSLR 120

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYR+KHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTG+VKIGDLG
Sbjct: 121 QYRRKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGDVKIGDLG 180

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDE+YNELVDIYSFGMCMLEIVTCEYPY+ECKNSAQ
Sbjct: 181 LAIVMQQPTARSVIGTPEFMAPELYDEEYNELVDIYSFGMCMLEIVTCEYPYSECKNSAQ 240

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHS E 
Sbjct: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSFEP 300

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
            RL NE  KS NPP+  SH MDTD N SK S SV SSVKS+N IS F+TLELQRL+E NE
Sbjct: 301 LRLSNEPFKSENPPVLASHSMDTDHNCSKFSGSVVSSVKSDNDISQFSTLELQRLTETNE 360

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKGDMTDHNT+SFHLRIAEL GKS+NIHFAFYLDSDTS+AI LEMVEQLELSNEDAT+
Sbjct: 361 LTLKGDMTDHNTISFHLRIAELSGKSKNIHFAFYLDSDTSLAIVLEMVEQLELSNEDATV 420

Query: 421 IAKLIDELIAKFVPSWKPCPNYCQEQNTSHSS--SETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVP  KPCPNY   QNT+HSS  SETQEDKTFIPPFFSE+VLSSPMVA 
Sbjct: 421 IAKLIDELIAKFVPDSKPCPNYYHGQNTTHSSSESETQEDKTFIPPFFSEMVLSSPMVAG 480

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           A  NNPT  TKVEDQEN QSIISCAS+++ +ST+SDYS GKG+                 
Sbjct: 481 AG-NNPTHFTKVEDQENLQSIISCASLDFNHSTISDYSTGKGT----------------- 540

Query: 541 GTIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDA 600
               +D +AVGSI TMIDFA PSLISSCSGMSKE+S S+FS+LSL+ERDHQDELKMEIDA
Sbjct: 541 ----MDFDAVGSISTMIDFANPSLISSCSGMSKEMSSSNFSSLSLEERDHQDELKMEIDA 600

Query: 601 IDLQYHQCLCELSRMREEAIETAKKRWMSKKKA 632
           IDLQYHQCLCELSRMREEAIE+AKKRWMSKKKA
Sbjct: 601 IDLQYHQCLCELSRMREEAIESAKKRWMSKKKA 609

BLAST of Lsi09G001480 vs. NCBI nr
Match: XP_038874814.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 591/635 (93.07%), Postives = 609/635 (95.91%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           MSCGSDLMMSGNSEADDREIVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 60

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLR
Sbjct: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKRKTINMITELFTSGSLR 120

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNP IIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPAIIHRDLKCDNIFVNGNTGEVKIGDLG 180

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL
Sbjct: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SRLLNEQ KS NPPL  SHPMDTDPN SKLS SV SSVKSNNGISHF+TLELQRL+ENN 
Sbjct: 301 SRLLNEQFKSANPPLIDSHPMDTDPNCSKLSGSVASSVKSNNGISHFSTLELQRLTENNV 360

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKGDMTDHNTMSFHLRIAELYGKS+NIHFAFYL+SDTS+AIALEMVEQLELSNEDATI
Sbjct: 361 LTLKGDMTDHNTMSFHLRIAELYGKSKNIHFAFYLNSDTSLAIALEMVEQLELSNEDATI 420

Query: 421 IAKLIDELIAKFVPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMV-AAA 480
           IAKLIDELIAKFVPSWKPCPNYC+EQNT +SSSET E+KTFIPPFFSELVLSSPMV AAA
Sbjct: 421 IAKLIDELIAKFVPSWKPCPNYCEEQNTPNSSSETPEEKTFIPPFFSELVLSSPMVAAAA 480

Query: 481 ARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNG 540
           ARNNP DL KVEDQENQQSIISCASVEYIYSTVSDYS+GKGSECGEFGHPECDKAYIS+G
Sbjct: 481 ARNNPRDLDKVEDQENQQSIISCASVEYIYSTVSDYSMGKGSECGEFGHPECDKAYISSG 540

Query: 541 TIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAI 600
           TIDLD+E VGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLS++ERDHQDELKMEIDAI
Sbjct: 541 TIDLDAEGVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAI 600

Query: 601 DLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           DLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 601 DLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 634

BLAST of Lsi09G001480 vs. NCBI nr
Match: KAA0042657.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa] >TYK06059.1 serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 580/637 (91.05%), Postives = 599/637 (94.03%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           MSCGSDL+MS NSEADDREIVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 1   MSCGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 60

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLR
Sbjct: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLR 120

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  ESPKEHSSEL
Sbjct: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSEL 300

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SR LNEQ KSVNPPL GSHPMDTD   +KLS SV SSVKSNNGISHF+T ELQRL+ENNE
Sbjct: 301 SRSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNE 360

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKG+MTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTS+AIALEMVEQLELSNEDATI
Sbjct: 361 LTLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATI 420

Query: 421 IAKLIDELIAKFVPSWKPCPNYCQE--QNTSHSSSETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVPSWKPCPNYC+E  QNT  S SE QE+K+FI PFFSELVLSSPMV  
Sbjct: 421 IAKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMV-V 480

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           AARNN T L KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHP+C+KAYIS+
Sbjct: 481 AARNNLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 540

Query: 541 GTIDLDSEAVGS-IPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEID 600
           GTIDLD+EAVGS I TMIDFAKPSLISSCSGMSKELSLSSFSTLS++ERDHQDELKMEID
Sbjct: 541 GTIDLDAEAVGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEID 600

Query: 601 AIDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           AIDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 601 AIDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Lsi09G001480 vs. NCBI nr
Match: XP_008437436.1 (PREDICTED: serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo])

HSP 1 Score: 1124.8 bits (2908), Expect = 0.0e+00
Identity = 579/637 (90.89%), Postives = 598/637 (93.88%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           MSCGSDL+MS NSEADDREIVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 1   MSCGSDLIMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 60

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLR
Sbjct: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLR 120

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  ESPKEHSSEL
Sbjct: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSEL 300

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SR LNEQ KSVNPPL GSHPMDTD   +KLS SV SSVKSNNGISHF+T ELQRL+ENNE
Sbjct: 301 SRSLNEQFKSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNE 360

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKG+MTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTS+AIALEMVEQLELSNEDATI
Sbjct: 361 LTLKGEMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATI 420

Query: 421 IAKLIDELIAKFVPSWKPCPNYCQE--QNTSHSSSETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVPSWKPCPNYC+E  QNT  S SE QE+K+FI PFFSELVLSSPMV  
Sbjct: 421 IAKLIDELIAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMV-V 480

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           AARNN T L KVEDQENQQSIISCAS EYIYSTVSDYSIGKGSECGEFGHP+C+KAYIS+
Sbjct: 481 AARNNLTGLAKVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 540

Query: 541 GTIDLDSEAVGS-IPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEID 600
           GTIDLD+EAVGS I TMIDFAKPSLISSCSGMSKELSLSSFSTLS++ERDHQDELKMEID
Sbjct: 541 GTIDLDAEAVGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEID 600

Query: 601 AIDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           AIDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 601 AIDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Lsi09G001480 vs. NCBI nr
Match: XP_004143946.1 (serine/threonine-protein kinase WNK8 isoform X1 [Cucumis sativus] >KGN49991.1 hypothetical protein Csa_000022 [Cucumis sativus])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 577/636 (90.72%), Postives = 598/636 (94.03%), Query Frame = 0

Query: 1   MSCGSDLMMSGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWS 60
           +SCGSDL+MSGNSEADDR IVEKDPSGRYIR      +  +  + YKAFDEVGGIEVAWS
Sbjct: 47  LSCGSDLIMSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTV-YKAFDEVGGIEVAWS 106

Query: 61  QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLR 120
           QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLR
Sbjct: 107 QVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLR 166

Query: 121 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 180
           QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG
Sbjct: 167 QYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLG 226

Query: 181 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 240
           LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ
Sbjct: 227 LAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQ 286

Query: 241 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSEL 300
           IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPAS RLPASELLKDPFLA ESPK++SSEL
Sbjct: 287 IFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSEL 346

Query: 301 SRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNE 360
           SR LNE  KSVNPPL GSHPM+TD N +KLS SV SSVKSNNGISHF+T ELQRL+ENNE
Sbjct: 347 SRSLNEHFKSVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNE 406

Query: 361 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATI 420
           LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTS+AIALEMVEQLELSNEDATI
Sbjct: 407 LTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATI 466

Query: 421 IAKLIDELIAKFVPSWKPCPNYC--QEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAA 480
           IAKLIDELIAKFVPSWKPCPNYC  Q+QNT H S E QEDKTFI PFFSELVLSSPMV A
Sbjct: 467 IAKLIDELIAKFVPSWKPCPNYCEEQQQNTPH-SPEAQEDKTFISPFFSELVLSSPMV-A 526

Query: 481 AARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISN 540
           AARNN T L KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHP+C+KAYIS+
Sbjct: 527 AARNNLTGLAKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISS 586

Query: 541 GTIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDA 600
           GTIDLD+EAVGS+ T IDFAKPSLISSCS MSKELSLSSFSTLS++ERDHQDELKMEIDA
Sbjct: 587 GTIDLDAEAVGSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDA 646

Query: 601 IDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           IDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 647 IDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 679

BLAST of Lsi09G001480 vs. NCBI nr
Match: XP_038874815.1 (serine/threonine-protein kinase WNK8-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 560/590 (94.92%), Postives = 575/590 (97.46%), Query Frame = 0

Query: 46  YKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQK 105
           YKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+K
Sbjct: 17  YKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKRK 76

Query: 106 TINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNI 165
           TINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNP IIHRDLKCDNI
Sbjct: 77  TINMITELFTSGSLRQYRKKHRKVDLKAFKNWARQILRGLTYLHGHNPAIIHRDLKCDNI 136

Query: 166 FVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEI 225
           FVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEI
Sbjct: 137 FVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEI 196

Query: 226 VTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKD 285
           VTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKD
Sbjct: 197 VTCEYPYNECKNSAQIFKKVTSGIKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKD 256

Query: 286 PFLATESPKEHSSELSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGIS 345
           PFLATESPKEHSSELSRLLNEQ KS NPPL  SHPMDTDPN SKLS SV SSVKSNNGIS
Sbjct: 257 PFLATESPKEHSSELSRLLNEQFKSANPPLIDSHPMDTDPNCSKLSGSVASSVKSNNGIS 316

Query: 346 HFTTLELQRLSENNELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIAL 405
           HF+TLELQRL+ENN LTLKGDMTDHNTMSFHLRIAELYGKS+NIHFAFYL+SDTS+AIAL
Sbjct: 317 HFSTLELQRLTENNVLTLKGDMTDHNTMSFHLRIAELYGKSKNIHFAFYLNSDTSLAIAL 376

Query: 406 EMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPF 465
           EMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYC+EQNT +SSSET E+KTFIPPF
Sbjct: 377 EMVEQLELSNEDATIIAKLIDELIAKFVPSWKPCPNYCEEQNTPNSSSETPEEKTFIPPF 436

Query: 466 FSELVLSSPMV-AAAARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECG 525
           FSELVLSSPMV AAAARNNP DL KVEDQENQQSIISCASVEYIYSTVSDYS+GKGSECG
Sbjct: 437 FSELVLSSPMVAAAAARNNPRDLDKVEDQENQQSIISCASVEYIYSTVSDYSMGKGSECG 496

Query: 526 EFGHPECDKAYISNGTIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLD 585
           EFGHPECDKAYIS+GTIDLD+E VGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLS++
Sbjct: 497 EFGHPECDKAYISSGTIDLDAEGVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSME 556

Query: 586 ERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIETAKKRWMSKKKATGI 635
           ERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIE+AKKRWMSKKKATGI
Sbjct: 557 ERDHQDELKMEIDAIDLQYHQCLCELSRMREEAIESAKKRWMSKKKATGI 606

BLAST of Lsi09G001480 vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 546.2 bits (1406), Expect = 3.5e-155
Identity = 320/618 (51.78%), Postives = 399/618 (64.56%), Query Frame = 0

Query: 13  SEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPE 72
           S  ++ +  EKDPSGRYIR      R  +  + YKAFDEV GIEVAW+ V IEDV+Q P 
Sbjct: 12  SSMEEADFAEKDPSGRYIRYDDVLGRGAFKTV-YKAFDEVDGIEVAWNLVSIEDVMQMPG 71

Query: 73  QLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLK 132
           QL+RLYSEVHLLK+LKHENIIK +  WVD+K KTINMITELFTSGSLR YRKKHRKVD K
Sbjct: 72  QLERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPK 131

Query: 133 AFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARS 192
           A KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTGEVKIGDLGLA V+QQPTARS
Sbjct: 132 AIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARS 191

Query: 193 VIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPA 252
           VIGTPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPYNEC+N AQI+KKVTS IKP 
Sbjct: 192 VIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQ 251

Query: 253 SLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQIKSVN 312
           SL KV DPQV+QFIEKCL+PAS R  A EL KDPFLA +  K+  S L    +   K V 
Sbjct: 252 SLGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKD--SALLASSSTSSKYVR 311

Query: 313 PPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNELTLKGDMTDHNT 372
           PP     PMD D N +K       SV SN       T+ELQR++EN E  L+G+ +D  T
Sbjct: 312 PPQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVT 371

Query: 373 MSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIAKF 432
            S  LRIA+  GK R +HFAFYL+SDT+ AIA EMVE+L L++++  +IA +ID+ I + 
Sbjct: 372 ASMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQL 431

Query: 433 VPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAAARNNPTDLTKVED 492
           +       +     +  +S   T ED                     A N  T  +K E+
Sbjct: 432 L------SDRTSSHHNQNSPRLTHEDH-------------------EAANQQTVNSKDEE 491

Query: 493 QENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNGTIDLDSEAVGSIP 552
               QS+ S  S +Y +     YS   G+   E G                D+E++    
Sbjct: 492 AAG-QSMKSDISADYYF----PYSANDGNAAMEAGR---------------DAESMS--- 551

Query: 553 TMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAIDLQYHQCLCELSR 612
                   S + SCS M      S+   LS+ + D+ ++LK E++ I+ Q++Q   +L +
Sbjct: 552 --------SYLDSCSMM------STIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLK 557

Query: 613 MREEAIETAKKRWMSKKK 631
           ++E+AIE AK++W++KK+
Sbjct: 612 LKEDAIENAKRKWITKKQ 557

BLAST of Lsi09G001480 vs. TAIR 10
Match: AT1G64630.1 (with no lysine (K) kinase 10 )

HSP 1 Score: 502.7 bits (1293), Expect = 4.4e-142
Identity = 300/611 (49.10%), Postives = 372/611 (60.88%), Query Frame = 0

Query: 16  DDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQ 75
           ++ + V+KDP+GRYIR      R  +  + YKAFDEV GIEVAW+ + IEDVLQ P QL 
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTV-YKAFDEVEGIEVAWNLMSIEDVLQMPGQLD 61

Query: 76  RLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKAFK 135
           RLYSEVHLL SLKH+NIIK +  WVDD  K+INMITELFTSGSL  YRKKHRKVD KA  
Sbjct: 62  RLYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIM 121

Query: 136 NWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIG 195
           NWARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG+VKIGDLGLA VMQQPTARSVIG
Sbjct: 122 NWARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIG 181

Query: 196 TPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLE 255
           TPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSGIKP SL 
Sbjct: 182 TPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLS 241

Query: 256 KVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQIKSVNPPL 315
           KV DPQVKQFIEKCL+PA  R  A ELLKD  LA +  K+  S L+   N   K   PP 
Sbjct: 242 KVDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKD--STLTASSNTTFKPAMPPQ 301

Query: 316 PGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENNELTLKGDMTDHNTMSF 375
               PMD +      S S+ SS KS+   +   T+E+QR++E+ E  L G+  D    S 
Sbjct: 302 CEYRPMDVE-YKKNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASM 361

Query: 376 HLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDATIIAKLIDELIAKFVPS 435
            LRIA   G++R + F F L +DT+ A+  EMVE+L+LS+ + T+IA++IDELI K    
Sbjct: 362 ALRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMK---- 421

Query: 436 WKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAAARNNPTDLTKVEDQEN 495
                                                  + A  +  N   + + +D+E 
Sbjct: 422 ---------------------------------------LKANRSLPNANSVYQSKDEEA 481

Query: 496 QQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNGTIDLDSEAVGSIPTMI 555
            +S+ S  S +Y +   S+     G  C                      EAV S+ +  
Sbjct: 482 GESMKSEISADYYHRVSSNEGSRLGCCC----------------------EAVESLLS-- 521

Query: 556 DFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAIDLQYHQCLCELSRMRE 615
                S + SCS +S + S               ++LK E++ I+ QY+Q    L RM+E
Sbjct: 542 -----SFLDSCSMVSNKQS---------------EDLKTELNVIESQYNQSCQRLLRMKE 521

Query: 616 EAIETAKKRWM 627
           EAIE AK++WM
Sbjct: 602 EAIEKAKRKWM 521

BLAST of Lsi09G001480 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 482.3 bits (1240), Expect = 6.2e-136
Identity = 257/442 (58.14%), Postives = 312/442 (70.59%), Query Frame = 0

Query: 10  SGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQ 69
           S   E  D E++E DP+ RYIR      +  +  + YKAFDEV GIEVAW+QV I+DVLQ
Sbjct: 8   SALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTV-YKAFDEVDGIEVAWNQVRIDDVLQ 67

Query: 70  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKV 129
           SP  L+RLYSEV LLKSLKH NII+FY+ W+DDK KT+N+ITELFTSGSLR YRKKHRKV
Sbjct: 68  SPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKV 127

Query: 130 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 189
           ++KA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q  
Sbjct: 128 NMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQAN 187

Query: 190 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 249
           A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV+SGI
Sbjct: 188 AKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGI 247

Query: 250 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES--------------PKE 309
           KPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL                  PKE
Sbjct: 248 KPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKE 307

Query: 310 HSSELSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRL 369
            +     L++E   +  P    S  +D D N   ++ S  S  +          +E++R 
Sbjct: 308 GAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSR---------CIEVRRA 367

Query: 370 SENNELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSN 429
              N   LKG+  D  ++S  LRI +  G+ RNIHF FY + DT+  ++ EMVEQLEL++
Sbjct: 368 KRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTD 427

Query: 430 EDATIIAKLIDELIAKFVPSWK 438
           ++ T IA+LID L+   +P+WK
Sbjct: 428 QNVTFIAELIDILLVNMIPTWK 439

BLAST of Lsi09G001480 vs. TAIR 10
Match: AT3G18750.3 (with no lysine (K) kinase 6 )

HSP 1 Score: 482.3 bits (1240), Expect = 6.2e-136
Identity = 257/442 (58.14%), Postives = 312/442 (70.59%), Query Frame = 0

Query: 10  SGNSEADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQ 69
           S   E  D E++E DP+ RYIR      +  +  + YKAFDEV GIEVAW+QV I+DVLQ
Sbjct: 8   SALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTV-YKAFDEVDGIEVAWNQVRIDDVLQ 67

Query: 70  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKV 129
           SP  L+RLYSEV LLKSLKH NII+FY+ W+DDK KT+N+ITELFTSGSLR YRKKHRKV
Sbjct: 68  SPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKV 127

Query: 130 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 189
           ++KA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q  
Sbjct: 128 NMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQAN 187

Query: 190 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 249
           A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV+SGI
Sbjct: 188 AKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGI 247

Query: 250 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES--------------PKE 309
           KPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL                  PKE
Sbjct: 248 KPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKE 307

Query: 310 HSSELSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRL 369
            +     L++E   +  P    S  +D D N   ++ S  S  +          +E++R 
Sbjct: 308 GAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSR---------CIEVRRA 367

Query: 370 SENNELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSN 429
              N   LKG+  D  ++S  LRI +  G+ RNIHF FY + DT+  ++ EMVEQLEL++
Sbjct: 368 KRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTD 427

Query: 430 EDATIIAKLIDELIAKFVPSWK 438
           ++ T IA+LID L+   +P+WK
Sbjct: 428 QNVTFIAELIDILLVNMIPTWK 439

BLAST of Lsi09G001480 vs. TAIR 10
Match: AT1G49160.2 (Protein kinase superfamily protein )

HSP 1 Score: 460.7 bits (1184), Expect = 1.9e-129
Identity = 282/630 (44.76%), Postives = 367/630 (58.25%), Query Frame = 0

Query: 14  EADDREIVEKDPSGRYIRGVLFDLRSVYVVIRYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 73
           E  D E++E DP+ RYIR      +     + +K FDEV GIEVAW+QV I+D+LQSP+ 
Sbjct: 12  EPPDPEVLEIDPTCRYIRYKEVIGKGASKTV-FKGFDEVDGIEVAWNQVRIDDLLQSPDC 71

Query: 74  LQRLYSEVHLLKSLKHENIIKFYSYWVDDKQKTINMITELFTSGSLRQYRKKHRKVDLKA 133
           L+RLYSEV LLKSLKH+NII+FY+ W+DDK KT+N+ITELFTSGSLRQYRKKHRKV++KA
Sbjct: 72  LERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKKHRKVNMKA 131

Query: 134 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSV 193
            K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN GEVKIGDLGLA VM+Q  A+SV
Sbjct: 132 VKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVMEQANAKSV 191

Query: 194 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPAS 253
           IGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT EYPY EC+NSAQI+KKV+SGIKPAS
Sbjct: 192 IGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKVSSGIKPAS 251

Query: 254 LEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATES--------------PKEHSSE 313
           L KV DP+V +FIEKCL+PAS RL A ELL D FL                  PKE S  
Sbjct: 252 LSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIVMPKEGSFG 311

Query: 314 LSRLLNEQIKSVNPPLPGSHPMDTDPNSSKLSASVGSSVKSNNGISHFTTLELQRLSENN 373
              L++E      PP   +  M  + +      ++   + SNN  S    +E++R    N
Sbjct: 312 ERCLMSE-----GPPNARNRTMSMNLDEDN---NLPIVISSNN--SGTNCIEVRRAKRGN 371

Query: 374 ELTLKGDMTDHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSMAIALEMVEQLELSNEDAT 433
              LKG+  D N++S  LRI +  G+ RNIHF F+ + DT+  ++ EMVEQLEL++++  
Sbjct: 372 FFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVK 431

Query: 434 IIAKLIDELIAKFVPSWKPCPNYCQEQNTSHSSSETQEDKTFIPPFFSELVLSSPMVAAA 493
            IA+LID L+   +P+WK               ++   D    P                
Sbjct: 432 FIAELIDVLLVNLIPNWK---------------TDVAVDHLIHP---------------- 491

Query: 494 ARNNPTDLTKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPECDKAYISNG 553
                        Q+NQ             S+  ++  G  S+ GE         Y    
Sbjct: 492 -------------QQNQ-------------SSKDNHQNGASSQAGESISHSLSSDY---- 551

Query: 554 TIDLDSEAVGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSLDERDHQDELKMEIDAI 613
                       P   D A P++ ++      E         SL+E +  + LK E++ I
Sbjct: 552 -----------CPRSDDEANPTVAATTEDQEAE------KPGSLEEEEEDERLKEELEKI 552

Query: 614 DLQYHQCLCELSRMREEAIETAKKRWMSKK 630
           + ++ + + E++R REEA    K R+  KK
Sbjct: 612 EERFREEMKEITRKREEATMETKNRFFEKK 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944Q04.9e-15451.78Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=... [more]
Q6EU492.2e-14649.92Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX... [more]
Q8RXE56.2e-14149.10Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8S8Y88.7e-13558.14Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8LST22.7e-12844.76Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7TLP60.0e+0091.05Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AUL50.0e+0090.89Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KQP10.0e+0090.72Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5... [more]
A0A1S4DS990.0e+0091.54Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1EQ951.1e-28983.25serine/threonine-protein kinase WNK8-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_038874814.10.0e+0093.07serine/threonine-protein kinase WNK8-like isoform X1 [Benincasa hispida][more]
KAA0042657.10.0e+0091.05serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa] ... [more]
XP_008437436.10.0e+0090.89PREDICTED: serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo][more]
XP_004143946.10.0e+0090.72serine/threonine-protein kinase WNK8 isoform X1 [Cucumis sativus] >KGN49991.1 hy... [more]
XP_038874815.10.0e+0094.92serine/threonine-protein kinase WNK8-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G41990.13.5e-15551.78with no lysine (K) kinase 8 [more]
AT1G64630.14.4e-14249.10with no lysine (K) kinase 10 [more]
AT3G18750.16.2e-13658.14with no lysine (K) kinase 6 [more]
AT3G18750.36.2e-13658.14with no lysine (K) kinase 6 [more]
AT1G49160.21.9e-12944.76Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 41..288
e-value: 1.2E-49
score: 180.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 56..288
e-value: 3.9E-46
score: 157.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 20..288
score: 36.809895
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 114..295
e-value: 1.3E-51
score: 176.7
NoneNo IPR availableGENE3D3.10.20.90coord: 371..472
e-value: 4.6E-7
score: 32.1
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 13..112
e-value: 1.1E-24
score: 88.3
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 76..270
e-value: 3.7E-10
score: 37.1
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 76..270
e-value: 3.7E-10
score: 37.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 321..335
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..335
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 12..629
NoneNo IPR availablePANTHERPTHR13902:SF139WNK KINASEcoord: 12..629
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 46..288
e-value: 1.56762E-156
score: 448.598
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 375..430
e-value: 9.2E-6
score: 25.6
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 155..167
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 46..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G001480.1Lsi09G001480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity