Lsi09G001300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi09G001300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	chr09: 1253990 .. 1257939 (+)
RNA-Seq Expression	Lsi09G001300
Synteny	Lsi09G001300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTTTCCCGTTTTCCGCTTTTTTACTTTACGCCGTCCATTTCAACGTTCTACAAATGACCACAACTGCACGGCTCTTCGCCTTGCTTCCGCCGCACACTCAACTCTCTGTCCACACTCACCGTCGCCTTCTCCGGCAACAGGCTCTCCGACGACGGTGTTTCTGTGCTGGCTCCAGATCGCTCCTTACTTTCACTCCTCAGTGCAGTGATTGGCATTTTCGGATTTCGAATTTCACTGAGTGTACTGAAGTTTAGCTCCATGGAGCTGAGAAGGCTCGGATTCAAGCAATGCGGATCGTTCGTTCTACTTCTGATTTCAGGTGATATTGAATTTCATGAACTTGCGCTTTTTTGTTGGTTTAAGATGTGGATTATTGTGAAATTGTTGAGTGTTGTTCAGTTGAAAACTTGAAACTATGTTGAATTTTGTGATTTTGTGAAGGTTTGTTGTTGCTGCCTGTTATGGTGAGTTCAGTTGGAGTGAACTACGGTCAAATAGCGAACAATCTTCCTGCTCCGGAGAATGTGATCCCGTTGGTGAAATCAATTGGAGCTACGAAGGTGAAGCTGTACGATGCGAGTCCGAAGGTTCTTCGCGCATTTGCTAATACAAGCGTGGAGTTCATAGTCGGACTTGGAAACGAGTACTTAGCTAAAATGAAGAATCCGGCATGTGCTGAAGAGTGGGTGAAGAATAATGTTCAAGCGTACTTCCCTGGCACTAAAATCACTTCAATCTTCGTCGGCAATGAGGTATTGACCTTCAATGACACTTCTCTTACTGCTAATTTACTACCGGCGATGCAGAGCGTGCACACGGCGCTCGTAAATCTAGGGCTAGATAAGCAGGTGGCCGTGACGACCGCGCATTCTTTGGCAATACTTGAAACTTCTTATCCGCCTTCCGCTGGAGTCTTCCGTAGGGATTTAGTTGATTGTTTAGTCCCAATCTTGGACTTCCATGTCAAGATCGGCTCTCCGTTCTTGATCAATGCGTACCCGTACTTTGCGTACAAGGCGAATCCGAAGCAGGTTTCGCTGGATTTCGTCCTCTTCCAACCGAACCAAGGGGTTTTAGATCCCGGCTCGAATCTTCACTACGACAACATGTTGTTCGCACAGATCGACGCGGTTTACTATGCGCTTGCCGCTATTGGTTACAAGAAACTTCCTGTTCATATATCGGAGACCGGATGGCCGTCGAAGGGAGACGAAGACGAGGCCGGTGCGACTCCGGAGAATGCAAAGAAGTACAATGGGAACCTACTCAAGTTCATCTGCCAGAAGAAGGGAACGCCATTGAGGCCGAGCTCGGATCTCAACATTTATGTGTTTGCTCTGTTTAACGAGAATATGAAGCCTGGACCAACTTCGGAGAGGAATTATGGGCTCTTTAAGCCTGATGGTACGCCGGTGTATCAGCTCGGATTTTCCATTAACGACGCCGTTGGAAACGGAACTCATGGCACTTCCAGCGCGCAACCTTCGCCGCCGGGACCTCCCACTACCTCCACTGGTTATTTGGCCATTTCCTCCGCCACGGTAATTTTTTTTTCTGTTACCTTTATTGTTTCAATGATTATTGTATCTTTTTTGCTTGATTTGCGTTGGGTTCATAAAGTGACAAAGATAGAATTAGAAAACAAATATAGGGTTAAGAAAATTTTGATTTTGCAATCGTTTAAATTACAAACTTAGTCCCTATAGTTTCAAAAAGAAATTAAAATTTAGTTCTTACTATTTTATAAAGCTTTATAAATAGTTCTTATCTAAGTTCTAGCTTTTACCAAATTATAGGAATCAAATTTAGAACATAATTTTTTTTTTTTGCAATTTCCATCTCTTACATCTAAGATCTTAATTCGGGGGAGAAACTTTCTGATCACTGGAAATTGAGAAAATTGTTGTTTTTAAAAAATGGAAAATAAAGTAATGAGAGAAATGAAAGAATAGGAAATTAGAGAATGTGAATATGAATTGGAAAGTGTTTGAGTGGGGGTCATTTCTCTGATTTCATTGGTGGGAGCGCATGATACATTAGCCCATTCGTCACTCATTGGATTCTCATCAGTTAATTCTTAGGCCACTCTAACATTTTAATTTTCAGCCGTATGCATATCCTTAGTCCCTGTGTCGTGGTCCAGAACAAAATTTGTCTTTATGATTATTTCTTACCTTGGCTGCACAAGAAAAATGGGTATAATTCGGTAGTTTTCCTGAACTTAATTGTTAAAATAGACATTCCAATTTCTTTTGGGAACTCAAGCTATCGAACCAATTAGGTTGCAAAATGAGCTCAAGTTCAGAATTCCCTTTTAGGATCCTTTTATGCAATACAATGCCATTGTAGAGCTCAAATTTCCTCTTTTTCCTTGATGTATATTGAAAATTTACTTGCAACTAACCGAATCTTCAATTATTCAAAAGAAATTAAGTGACTGGATACATTAGAACATTAATGTATGTGTTTGCGTTACTGTTTGTGGAGACTGAGAAACAGTGATGATTTTTTTATGGCAGGAGAGAAGCCATAGAGTTGGATTCTTCTCAGCATGCCTGTTTCTGATGCTGTTCAAGTTGCTATTTTGAATTTAGGAGATTATTTATTTTTCAGTGTATGTTTCCGAAAGATGCTTTGGATTTATTTCATTTATCAGAATCAGCCGTTAAAAGCAAATTGGAAGATGCTAAAGAAAATAAGAGGCAGCGTCGGATTTGGGAAAAGGTAAAAATTCTGTTCCTTTCCTTGTACGCGTCTACCTTCTCTCTTTTGTTTCTCTGCCTTCCTTTTTGCCATTCTTTTGTGTTGTTTGTGACCATAACAAAAGTTATTCGGCTTCTCCTTTACTGCTACTAACAAAAGGTAGAAATTATGAATTGAAACCATTTTTTTTTTTTTTTGTAGTATCTAAAACAGATAATTTAGCCCGTTTAAGTAATGATTTTATTTTATAATATAGGCATTTTCTTTCGATTTCTATACTATGATTTTTCCATTTCTTAAAAGGATACTTGTTCTTAGTGAAATTCTTAAAATAAAGATAACTTTTAAAAGCTATCTTTTTTTTAGTTTTCAAAACTTGATTCATTGATAGAAAGTAGGTGGAACGTAGGAATAGTGATTATAAGCTTAATTTTCAAAATAACAAAAACTAAATATCAAATAGTGATGAAACGGAACTTAAGTGAGGGGAAATTCCAATACCATGTAGGCCTCGTATTCTCTTTGGCAATAATCATAAAAAATCTGGAAGATAGAAAAAGGAACCCTATTCGCCATACTTTTTTCAATTTAGGTGAAGAGTGCTGTTGTTATATTTTGGATATTTTTCTATACTTGGTATCTTTGCTTGCCTACCTTATATTTTCGGGTTTATGTGTTTTATATATTCTTTTATAGTTGTAAAAAGGAAGGTTTGATCATGCAAAACTTTGTTTGGTAATAGCTAAACATTTTTATGATATGATATAATATAAAAGCAACAATATCAATGAAAGTTTCTTGAATTTACCACTAGTCACTTAATGGAGTGGGGGCAAACAACTGACAAAGCCATGTGACAAAAGATAAAGTTCAGTTTGGAATTTTTATCATTTCCTTTTTTGTATGCATTCCATTTGGTATTCTGAGCTGGATCTGATCTGTTAATATCTTTTTTTTCTTATACTGCTTTTTTACAGGGATATATCAGTTTTTTAGTAATTAATAATTTTGATGTGAATTGGGTTCGTGGATGGACATAAAATCTGGTGGATGGACGATCCAAACTTGTTCTTTTGTAATGCCATTTGGTATCTAATTTTATTGATTTGCCTATTTTTATGTACCTCTCTCCTCTATTCTCCACTTTTGATTCTAGTTGGACTTTTGTTAAATATTCAGAAATATATTGGATCTGTCGTTTTCTTTTACAAGGATACCAATGCAATTGTGAAAATTTGAACCG

mRNA sequence

TTTTTTTTCCCGTTTTCCGCTTTTTTACTTTACGCCGTCCATTTCAACGTTCTACAAATGACCACAACTGCACGGCTCTTCGCCTTGCTTCCGCCGCACACTCAACTCTCTGTCCACACTCACCGTCGCCTTCTCCGGCAACAGGCTCTCCGACGACGGTGTTTCTGTGCTGGCTCCAGATCGCTCCTTACTTTCACTCCTCAGTGCAGTGATTGGCATTTTCGGATTTCGAATTTCACTGAGTGTACTGAAGTTTAGCTCCATGGAGCTGAGAAGGCTCGGATTCAAGCAATGCGGATCGTTCGTTCTACTTCTGATTTCAGGTTTGTTGTTGCTGCCTGTTATGGTGAGTTCAGTTGGAGTGAACTACGGTCAAATAGCGAACAATCTTCCTGCTCCGGAGAATGTGATCCCGTTGGTGAAATCAATTGGAGCTACGAAGGTGAAGCTGTACGATGCGAGTCCGAAGGTTCTTCGCGCATTTGCTAATACAAGCGTGGAGTTCATAGTCGGACTTGGAAACGAGTACTTAGCTAAAATGAAGAATCCGGCATGTGCTGAAGAGTGGGTGAAGAATAATGTTCAAGCGTACTTCCCTGGCACTAAAATCACTTCAATCTTCGTCGGCAATGAGGTATTGACCTTCAATGACACTTCTCTTACTGCTAATTTACTACCGGCGATGCAGAGCGTGCACACGGCGCTCGTAAATCTAGGGCTAGATAAGCAGGTGGCCGTGACGACCGCGCATTCTTTGGCAATACTTGAAACTTCTTATCCGCCTTCCGCTGGAGTCTTCCGTAGGGATTTAGTTGATTGTTTAGTCCCAATCTTGGACTTCCATGTCAAGATCGGCTCTCCGTTCTTGATCAATGCGTACCCGTACTTTGCGTACAAGGCGAATCCGAAGCAGGTTTCGCTGGATTTCGTCCTCTTCCAACCGAACCAAGGGGTTTTAGATCCCGGCTCGAATCTTCACTACGACAACATGTTGTTCGCACAGATCGACGCGGTTTACTATGCGCTTGCCGCTATTGGTTACAAGAAACTTCCTGTTCATATATCGGAGACCGGATGGCCGTCGAAGGGAGACGAAGACGAGGCCGGTGCGACTCCGGAGAATGCAAAGAAGTACAATGGGAACCTACTCAAGTTCATCTGCCAGAAGAAGGGAACGCCATTGAGGCCGAGCTCGGATCTCAACATTTATGTGTTTGCTCTGTTTAACGAGAATATGAAGCCTGGACCAACTTCGGAGAGGAATTATGGGCTCTTTAAGCCTGATGGTACGCCGGTGTATCAGCTCGGATTTTCCATTAACGACGCCGTTGGAAACGGAACTCATGGCACTTCCAGCGCGCAACCTTCGCCGCCGGGACCTCCCACTACCTCCACTGGTTATTTGGCCATTTCCTCCGCCACGGAGAGAAGCCATAGAGTTGGATTCTTCTCAGCATGCCTGTTTCTGATGCTGTTCAAGTTGCTATTTTGAATTTAGGAGATTATTTATTTTTCAGTGTATGTTTCCGAAAGATGCTTTGGATTTATTTCATTTATCAGAATCAGCCGTTAAAAGCAAATTGGAAGATGCTAAAGAAAATAAGAGGCAGCGTCGGATTTGGGAAAAGGGATATATCAGTTTTTTAGTAATTAATAATTTTGATGTGAATTGGGTTCGTGGATGGACATAAAATCTGGTGGATGGACGATCCAAACTTGTTCTTTTGTAATGCCATTTGGTATCTAATTTTATTGATTTGCCTATTTTTATGTACCTCTCTCCTCTATTCTCCACTTTTGATTCTAGTTGGACTTTTGTTAAATATTCAGAAATATATTGGATCTGTCGTTTTCTTTTACAAGGATACCAATGCAATTGTGAAAATTTGAACCG

Coding sequence (CDS)

ATGGAGCTGAGAAGGCTCGGATTCAAGCAATGCGGATCGTTCGTTCTACTTCTGATTTCAGGTTTGTTGTTGCTGCCTGTTATGGTGAGTTCAGTTGGAGTGAACTACGGTCAAATAGCGAACAATCTTCCTGCTCCGGAGAATGTGATCCCGTTGGTGAAATCAATTGGAGCTACGAAGGTGAAGCTGTACGATGCGAGTCCGAAGGTTCTTCGCGCATTTGCTAATACAAGCGTGGAGTTCATAGTCGGACTTGGAAACGAGTACTTAGCTAAAATGAAGAATCCGGCATGTGCTGAAGAGTGGGTGAAGAATAATGTTCAAGCGTACTTCCCTGGCACTAAAATCACTTCAATCTTCGTCGGCAATGAGGTATTGACCTTCAATGACACTTCTCTTACTGCTAATTTACTACCGGCGATGCAGAGCGTGCACACGGCGCTCGTAAATCTAGGGCTAGATAAGCAGGTGGCCGTGACGACCGCGCATTCTTTGGCAATACTTGAAACTTCTTATCCGCCTTCCGCTGGAGTCTTCCGTAGGGATTTAGTTGATTGTTTAGTCCCAATCTTGGACTTCCATGTCAAGATCGGCTCTCCGTTCTTGATCAATGCGTACCCGTACTTTGCGTACAAGGCGAATCCGAAGCAGGTTTCGCTGGATTTCGTCCTCTTCCAACCGAACCAAGGGGTTTTAGATCCCGGCTCGAATCTTCACTACGACAACATGTTGTTCGCACAGATCGACGCGGTTTACTATGCGCTTGCCGCTATTGGTTACAAGAAACTTCCTGTTCATATATCGGAGACCGGATGGCCGTCGAAGGGAGACGAAGACGAGGCCGGTGCGACTCCGGAGAATGCAAAGAAGTACAATGGGAACCTACTCAAGTTCATCTGCCAGAAGAAGGGAACGCCATTGAGGCCGAGCTCGGATCTCAACATTTATGTGTTTGCTCTGTTTAACGAGAATATGAAGCCTGGACCAACTTCGGAGAGGAATTATGGGCTCTTTAAGCCTGATGGTACGCCGGTGTATCAGCTCGGATTTTCCATTAACGACGCCGTTGGAAACGGAACTCATGGCACTTCCAGCGCGCAACCTTCGCCGCCGGGACCTCCCACTACCTCCACTGGTTATTTGGCCATTTCCTCCGCCACGGAGAGAAGCCATAGAGTTGGATTCTTCTCAGCATGCCTGTTTCTGATGCTGTTCAAGTTGCTATTTTGA

Protein sequence

MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLLF

Homology

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BLAST of Lsi09G001300 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.5e-143
Identity = 257/413 (62.23%), Postives = 318/413 (77.00%), Query Frame = 0


Query: 13  SFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLR 72
           S + L+   L++LP   +S+GVNYGQI +NLP+P +VIPL+KSIGATKVKLYDA+P++L+
Sbjct: 10  SLLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILK 69

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTS 132
           AF+NT +EFI+GLGNEYL+KMK+P+ A  W+K NV  + P T IT I +GNE+L  ND+S
Sbjct: 70  AFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSS 129

Query: 133 LTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILD 192
           LT NLLPAMQ VH+AL+  GL  Q++VTTAHSL+IL++S+PPSAG F+ DL+D L PIL+
Sbjct: 130 LTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILE 189

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FH K  SPFLINAYP+FAYK NPK+V LDFVLFQPNQG++DP +  HYDNMLFAQIDAVY
Sbjct: 190 FHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVY 249

Query: 253 YALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC--QKKGTPLRPS 312
            ALAA G+K L V ISETGWPSKGD+DE GATPENAK+YNGNL+K +   +K  TPL+P+
Sbjct: 250 SALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPN 309

Query: 313 SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAV----GNGTHGTSSA 372
           +DL+IYVFALFNEN+KPGPTSERNYGLFKPDGT  Y LGF++ND V    G GT G +S+
Sbjct: 310 NDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRGASGGGTGGGNSS 369

Query: 373 Q-----------PSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
                        SP  P + STGYLAIS++     R G   A L L++  LL
Sbjct: 370 SGGGRDKSPVFPVSPVAPDSASTGYLAISASPVTGKRKG-KGAILSLVVSMLL 421

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BLAST of Lsi09G001300 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 6.6e-136
Identity = 254/417 (60.91%), Postives = 300/417 (71.94%), Query Frame = 0


Query: 13  SFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLR 72
           S   L    L  LP++VSS+G+NYGQ+ANNLP P+NVIPL+KS+GATKVKLYDA P+ LR
Sbjct: 8   SLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALR 67

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTS 132
           AFA +  E  V LGNEYLA+M +P  A+ WVK NVQAY P TKI +I VGNEVLT N ++
Sbjct: 68  AFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 127

Query: 133 LTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILD 192
           LTA L PAMQS+H ALV+ GL+KQ+ VTTAHSLAIL+ SYPPSA  FRRDL+  L PILD
Sbjct: 128 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 187

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FHVK GSP LINAYP+FAY+ NPK VSLDFVLFQPNQG  DPGSN HYDNMLFAQ+DAVY
Sbjct: 188 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 247

Query: 253 YALAAIG--YKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKK-GTPLRP 312
           +AL A+G  YKK+P+ +SETGWPS GD  E GAT +NA+KYNGNL+K +  KK  TP+RP
Sbjct: 248 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 307

Query: 313 SSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSSAQPS 372
             DL I+VFALFNENMKPGPTSERNYGLF PDGTPVY LG   +    +G+  ++S   S
Sbjct: 308 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGS 367

Query: 373 PPG---------------PPTT---STGYLAISSATERSHRVGFFSACLFLMLFKLL 409
             G                P T   S  Y++ISSA  +     F    LF  L  ++
Sbjct: 368 SSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGGKGR---FVECVLFFFLLCII 421

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BLAST of Lsi09G001300 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 421.8 bits (1083), Expect = 9.0e-117
Identity = 202/336 (60.12%), Postives = 260/336 (77.38%), Query Frame = 0


Query: 15  VLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLRAF 74
           VLLL+   L   +    VG+NYGQIANNLP+P  V  L++S+  T+VKLYDA P VL +F
Sbjct: 12  VLLLLFLTLSERIKGQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 71

Query: 75  ANTSVEFIVGLGNEYLAKMK-NPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTSL 134
           +N+ V+F++GLGNEYL  M  +P  A++W++  ++ +   T+ITSI VGNE+   ND  L
Sbjct: 72  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 131

Query: 135 TANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILDF 194
             +LLPAM+SV+ AL NLGL+KQV VT+AHSL IL TSYPPS+G F+ + +  L P+LDF
Sbjct: 132 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 191

Query: 195 HVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYY 254
           H +I SPFLINAYP+FAYK +PK+V L++VLFQPNQG++DP +NLHYDNMLFAQ+DA+Y 
Sbjct: 192 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 251

Query: 255 ALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDL 314
           A+  +G+  + V ISETGWPSKGDE+E GA+PENA  YNGNLLK I Q+KGTP + S  +
Sbjct: 252 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 311

Query: 315 NIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLG 350
           ++YVFALFNEN+KPGP SERNYGLF PDG PVY +G
Sbjct: 312 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVG 347

[Close]

BLAST of Lsi09G001300 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.8e-83
Identity = 154/349 (44.13%), Postives = 233/349 (66.76%), Query Frame = 0


Query: 28  MVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLRAFANTSVEFIVGLGN 87
           M S +G+ YG+ A+NLP+P  V  L++ +    V++YDA+  VL+AFANT +E ++G+ N
Sbjct: 23  MASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPN 82

Query: 88  -EYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTSLTANLLPAMQSVHT 147
            + LA  +  +  + W+ NN+  Y+P TKITSI VG EV    D + T  +LPAM+++HT
Sbjct: 83  ADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNA-TGLVLPAMRNIHT 142

Query: 148 ALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILDFHVKIGSPFLINAY 207
           AL   GLDK++ ++++HSLAIL  S+PPS+  F +     L P+L+F V+  SPF+I+ Y
Sbjct: 143 ALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLY 202

Query: 208 PYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYYALAAIGYKKLPVH 267
           PY+AY+ + ++V L++ LF+ +  V+DP + L Y NM  AQ+DA+Y+AL A+ +K + V 
Sbjct: 203 PYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVM 262

Query: 268 ISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDLNIYVFALFNENMK 327
           ++E+GWPSKG   E  ATPENA  YN NL++ +    GTP +P  ++++Y+F+LFNEN K
Sbjct: 263 VTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRK 322

Query: 328 PGPTSERNYGLFKPDGTPVYQLGFS-INDAVGNGTHGTSSAQPSPPGPP 375
           PG  SERN+G+F  +GT VY L F+  N    + T+ T+   PSP   P
Sbjct: 323 PGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSP 370

[Close]

BLAST of Lsi09G001300 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.7e-81
Identity = 153/342 (44.74%), Postives = 222/342 (64.91%), Query Frame = 0


Query: 30  SSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLRAFANTSVEFIVGLGNEY 89
           S +GVNYGQ+++NLP+ +  + L+KS    KV+L+ A P V++AFANT VE ++G  N  
Sbjct: 28  SFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGD 87

Query: 90  LAKM-KNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTSLTANLLPAMQSVHTAL 149
           +  +  NP  A ++VK+NV +++P + I +I VGNEVLT  D  L + LLPAMQ+V  AL
Sbjct: 88  IPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKLISQLLPAMQNVQNAL 147

Query: 150 VNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILDFHVKIGSPFLINAYPY 209
               L  +V V+T H++A+L  SYPPS+GVF   L D +  +L F     +PF+I+ YPY
Sbjct: 148 NAASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLGDTMKALLQFQSANDAPFMISPYPY 207

Query: 210 FAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYYALAAIGYKKLPVHIS 269
           FAYK  P   +L F LFQPN G +D G+   Y NM  AQ+DAV+ AL A+G+K + + ++
Sbjct: 208 FAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEIVVA 267

Query: 270 ETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDLNIYVFALFNENMKPG 329
           ETGWP  GD +E G + +NAK Y GNL+  +  K GTPL P   ++ Y+F+L++E+ K G
Sbjct: 268 ETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDKKTG 327

Query: 330 PTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSSAQPSP 371
            +SE+ +GLFKPDG+  Y +G      + N  + T+ A P+P
Sbjct: 328 ASSEKYFGLFKPDGSTTYDVGL-----LKNTQNPTTPATPTP 364

[Close]

BLAST of Lsi09G001300 vs. ExPASy TrEMBL
Match: A0A0A0KK78 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G146960 PE=3 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.4e-228
Identity = 399/408 (97.79%), Postives = 403/408 (98.77%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK
Sbjct: 1   MELRRFGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACAEEWVKNNVQAYFPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSIND VGNGT
Sbjct: 301 QKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDGVGNGT 360

Query: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           HGTSSAQPSPPGPPTTSTGYLAISSATERSH VGFFS  LFLM+FKLL
Sbjct: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHWVGFFSMFLFLMVFKLL 408

[Close]

BLAST of Lsi09G001300 vs. ExPASy TrEMBL
Match: A0A5A7TM33 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001430 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.7e-223
Identity = 393/409 (96.09%), Postives = 399/409 (97.56%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISG-LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60
           MELRR GFKQCGS VLLLISG LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT
Sbjct: 1   MELRRFGFKQCGSLVLLLISGLLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60

Query: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSI 120
           KVKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACAEEWVKNNVQAYFPGTKITSI
Sbjct: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSI 120

Query: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180
           FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF
Sbjct: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180

Query: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLH 240
           RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNL 
Sbjct: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLR 240

Query: 241 YDNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFI 300
           YDNMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGD+DEAGATPENAKKYNGNLLKFI
Sbjct: 241 YDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDDDEAGATPENAKKYNGNLLKFI 300

Query: 301 CQKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNG 360
           CQKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF+IND VGNG
Sbjct: 301 CQKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFTINDGVGNG 360

Query: 361 THGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           THGTSSAQPSPPG PTTSTGYLAISSATERSH VGFFS  LFL +FKLL
Sbjct: 361 THGTSSAQPSPPGTPTTSTGYLAISSATERSHWVGFFSMSLFLTVFKLL 409

[Close]

BLAST of Lsi09G001300 vs. ExPASy TrEMBL
Match: A0A1S3ATQ5 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482869 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.7e-223
Identity = 393/409 (96.09%), Postives = 399/409 (97.56%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISG-LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60
           MELRR GFKQCGS VLLLISG LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT
Sbjct: 1   MELRRFGFKQCGSLVLLLISGLLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60

Query: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSI 120
           KVKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACAEEWVKNNVQAYFPGTKITSI
Sbjct: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSI 120

Query: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180
           FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF
Sbjct: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180

Query: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLH 240
           RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNL 
Sbjct: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLR 240

Query: 241 YDNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFI 300
           YDNMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGD+DEAGATPENAKKYNGNLLKFI
Sbjct: 241 YDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDDDEAGATPENAKKYNGNLLKFI 300

Query: 301 CQKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNG 360
           CQKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF+IND VGNG
Sbjct: 301 CQKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFTINDGVGNG 360

Query: 361 THGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           THGTSSAQPSPPG PTTSTGYLAISSATERSH VGFFS  LFL +FKLL
Sbjct: 361 THGTSSAQPSPPGTPTTSTGYLAISSATERSHWVGFFSMSLFLTVFKLL 409

[Close]

BLAST of Lsi09G001300 vs. ExPASy TrEMBL
Match: A0A6J1H1N2 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459311 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.0e-220
Identity = 384/411 (93.43%), Postives = 399/411 (97.08%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GF+QCGSFVLLL+SGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVK+IGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACA+EWVKNNVQAY PGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 H---GTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           H   GTS  QP+PPG PTTSTGYL+ISSA ERS  +GFFS CL+LMLFKLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSRCLGFFSVCLYLMLFKLL 411

[Close]

BLAST of Lsi09G001300 vs. ExPASy TrEMBL
Match: A0A6J1K8L2 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491117 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.2e-218
Identity = 382/411 (92.94%), Postives = 397/411 (96.59%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GF+QCGS VLLL+SGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVK+IGATK
Sbjct: 1   MELRRFGFEQCGS-VLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACA+EWVKNNVQAY PGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQVAVTTAHSLA+LETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAVLETSYPPSAGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 H---GTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           H   GTS  QP+PPG PTTS GYL+ISSA ERSH +GFF  CL+LMLFKLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSNGYLSISSAKERSHCLGFFLVCLYLMLFKLL 410

[Close]

BLAST of Lsi09G001300 vs. NCBI nr
Match: XP_011654675.1 (glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN49973.1 hypothetical protein Csa_000456 [Cucumis sativus])

HSP 1 Score: 800.4 bits (2066), Expect = 7.1e-228
Identity = 399/408 (97.79%), Postives = 403/408 (98.77%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK
Sbjct: 1   MELRRFGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACAEEWVKNNVQAYFPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSIND VGNGT
Sbjct: 301 QKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDGVGNGT 360

Query: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           HGTSSAQPSPPGPPTTSTGYLAISSATERSH VGFFS  LFLM+FKLL
Sbjct: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHWVGFFSMFLFLMVFKLL 408

[Close]

BLAST of Lsi09G001300 vs. NCBI nr
Match: XP_038906881.1 (glucan endo-1,3-beta-glucosidase 11 [Benincasa hispida])

HSP 1 Score: 798.5 bits (2061), Expect = 2.7e-227
Identity = 398/408 (97.55%), Postives = 402/408 (98.53%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GFK CGSFVLLL+SGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK
Sbjct: 1   MELRRFGFKHCGSFVLLLVSGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR
Sbjct: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           DNMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGAT ENAKKYNGNLLKFIC
Sbjct: 241 DNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATQENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 HGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           HGTSS+QPSPPGPPTTSTGYLAISSA ER+HRVGF SA LFLMLFKLL
Sbjct: 361 HGTSSSQPSPPGPPTTSTGYLAISSAMERNHRVGFLSALLFLMLFKLL 408

[Close]

BLAST of Lsi09G001300 vs. NCBI nr
Match: XP_008437454.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo] >KAA0042641.1 glucan endo-1,3-beta-glucosidase 11 [Cucumis melo var. makuwa] >TYK06042.1 glucan endo-1,3-beta-glucosidase 11 [Cucumis melo var. makuwa])

HSP 1 Score: 783.1 bits (2021), Expect = 1.2e-222
Identity = 393/409 (96.09%), Postives = 399/409 (97.56%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISG-LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60
           MELRR GFKQCGS VLLLISG LLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT
Sbjct: 1   MELRRFGFKQCGSLVLLLISGLLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGAT 60

Query: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSI 120
           KVKLYDASPKVLRAFANTSVEFIVGLGNEYL+KMKNPACAEEWVKNNVQAYFPGTKITSI
Sbjct: 61  KVKLYDASPKVLRAFANTSVEFIVGLGNEYLSKMKNPACAEEWVKNNVQAYFPGTKITSI 120

Query: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180
           FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF
Sbjct: 121 FVGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVF 180

Query: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLH 240
           RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNL 
Sbjct: 181 RRDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLR 240

Query: 241 YDNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFI 300
           YDNMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGD+DEAGATPENAKKYNGNLLKFI
Sbjct: 241 YDNMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDDDEAGATPENAKKYNGNLLKFI 300

Query: 301 CQKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNG 360
           CQKKGTPLRP+SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGF+IND VGNG
Sbjct: 301 CQKKGTPLRPNSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFTINDGVGNG 360

Query: 361 THGTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           THGTSSAQPSPPG PTTSTGYLAISSATERSH VGFFS  LFL +FKLL
Sbjct: 361 THGTSSAQPSPPGTPTTSTGYLAISSATERSHWVGFFSMSLFLTVFKLL 409

[Close]

BLAST of Lsi09G001300 vs. NCBI nr
Match: XP_023534433.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 777.7 bits (2007), Expect = 4.9e-221
Identity = 385/411 (93.67%), Postives = 400/411 (97.32%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GF+QCGSFVLLL+SGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVK+IGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMKNPACA+EWVKNNVQAY PGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKNPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 H---GTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           H   GTS  QP+PPG PTTSTGYL+ISSA ERSH +GFFS CL+LMLFKLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSHCLGFFSVCLYLMLFKLL 411

[Close]

BLAST of Lsi09G001300 vs. NCBI nr
Match: KAG6605611.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 777.7 bits (2007), Expect = 4.9e-221
Identity = 385/411 (93.67%), Postives = 401/411 (97.57%), Query Frame = 0


Query: 1   MELRRLGFKQCGSFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATK 60
           MELRR GF+QCGSFVLLL+SGLLLLPVMVSS+GVNYGQIANNLPAPENVIPLVK+IGATK
Sbjct: 1   MELRRFGFEQCGSFVLLLVSGLLLLPVMVSSIGVNYGQIANNLPAPENVIPLVKAIGATK 60

Query: 61  VKLYDASPKVLRAFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIF 120
           VKLYDASPKVLRAF+NTSVEFIVGLGNEYLAKMK+PACA+EWVKNNVQAY PGTKITSIF
Sbjct: 61  VKLYDASPKVLRAFSNTSVEFIVGLGNEYLAKMKSPACAQEWVKNNVQAYLPGTKITSIF 120

Query: 121 VGNEVLTFNDTSLTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFR 180
           VGNEVLTFNDTSL+ANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPS+GVFR
Sbjct: 121 VGNEVLTFNDTSLSANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSSGVFR 180

Query: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHY 240
           RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQG+LDPGSNLHY
Sbjct: 181 RDLVDCLVPILDFHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGILDPGSNLHY 240

Query: 241 DNMLFAQIDAVYYALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC 300
           +NMLFAQIDAVYYALAA+GYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNL+KFIC
Sbjct: 241 ENMLFAQIDAVYYALAAVGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLIKFIC 300

Query: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360
           QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT
Sbjct: 301 QKKGTPLRPSSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGT 360

Query: 361 H---GTSSAQPSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
           H   GTS  QP+PPG PTTSTGYL+ISSA ERSHR+GFFSACL LMLFKLL
Sbjct: 361 HGTSGTSDVQPTPPGFPTTSTGYLSISSAKERSHRLGFFSACLSLMLFKLL 411

[Close]

BLAST of Lsi09G001300 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.0e-144
Identity = 257/413 (62.23%), Postives = 318/413 (77.00%), Query Frame = 0


Query: 13  SFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLR 72
           S + L+   L++LP   +S+GVNYGQI +NLP+P +VIPL+KSIGATKVKLYDA+P++L+
Sbjct: 10  SLLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILK 69

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTS 132
           AF+NT +EFI+GLGNEYL+KMK+P+ A  W+K NV  + P T IT I +GNE+L  ND+S
Sbjct: 70  AFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSS 129

Query: 133 LTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILD 192
           LT NLLPAMQ VH+AL+  GL  Q++VTTAHSL+IL++S+PPSAG F+ DL+D L PIL+
Sbjct: 130 LTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILE 189

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FH K  SPFLINAYP+FAYK NPK+V LDFVLFQPNQG++DP +  HYDNMLFAQIDAVY
Sbjct: 190 FHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVY 249

Query: 253 YALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFIC--QKKGTPLRPS 312
            ALAA G+K L V ISETGWPSKGD+DE GATPENAK+YNGNL+K +   +K  TPL+P+
Sbjct: 250 SALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPN 309

Query: 313 SDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAV----GNGTHGTSSA 372
           +DL+IYVFALFNEN+KPGPTSERNYGLFKPDGT  Y LGF++ND V    G GT G +S+
Sbjct: 310 NDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRGASGGGTGGGNSS 369

Query: 373 Q-----------PSPPGPPTTSTGYLAISSATERSHRVGFFSACLFLMLFKLL 409
                        SP  P + STGYLAIS++     R G   A L L++  LL
Sbjct: 370 SGGGRDKSPVFPVSPVAPDSASTGYLAISASPVTGKRKG-KGAILSLVVSMLL 421

[Close]

BLAST of Lsi09G001300 vs. TAIR 10
Match: AT5G42100.2 (beta-1,3-glucanase_putative )

HSP 1 Score: 485.7 bits (1249), Expect = 3.6e-137
Identity = 254/414 (61.35%), Postives = 299/414 (72.22%), Query Frame = 0


Query: 13  SFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLR 72
           S   L    L  LP++VSS+G+NYGQ+ANNLP P+NVIPL+KS+GATKVKLYDA P+ LR
Sbjct: 8   SLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALR 67

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTS 132
           AFA +  E  V LGNEYLA+M +P  A+ WVK NVQAY P TKI +I VGNEVLT N ++
Sbjct: 68  AFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 127

Query: 133 LTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILD 192
           LTA L PAMQS+H ALV+ GL+KQ+ VTTAHSLAIL+ SYPPSA  FRRDL+  L PILD
Sbjct: 128 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 187

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FHVK GSP LINAYP+FAY+ NPK VSLDFVLFQPNQG  DPGSN HYDNMLFAQ+DAVY
Sbjct: 188 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 247

Query: 253 YALAAIG--YKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKK-GTPLRP 312
           +AL A+G  YKK+P+ +SETGWPS GD  E GAT +NA+KYNGNL+K +  KK  TP+RP
Sbjct: 248 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 307

Query: 313 SSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSSAQPS 372
             DL I+VFALFNENMKPGPTSERNYGLF PDGTPVY LG   +    +G+  ++S   S
Sbjct: 308 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGS 367

Query: 373 PPG---------------PPTT---STGYLAISSATERSHRVGFFSACLFLMLF 406
             G                P T   S  Y++ISSA      + F   C  L +F
Sbjct: 368 SSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGVTFSTLHFI-CCFTLTIF 420

[Close]

BLAST of Lsi09G001300 vs. TAIR 10
Match: AT5G42100.1 (beta-1,3-glucanase_putative )

HSP 1 Score: 485.3 bits (1248), Expect = 4.7e-137
Identity = 254/417 (60.91%), Postives = 300/417 (71.94%), Query Frame = 0


Query: 13  SFVLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLR 72
           S   L    L  LP++VSS+G+NYGQ+ANNLP P+NVIPL+KS+GATKVKLYDA P+ LR
Sbjct: 8   SLFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALR 67

Query: 73  AFANTSVEFIVGLGNEYLAKMKNPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTS 132
           AFA +  E  V LGNEYLA+M +P  A+ WVK NVQAY P TKI +I VGNEVLT N ++
Sbjct: 68  AFAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 127

Query: 133 LTANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILD 192
           LTA L PAMQS+H ALV+ GL+KQ+ VTTAHSLAIL+ SYPPSA  FRRDL+  L PILD
Sbjct: 128 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 187

Query: 193 FHVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVY 252
           FHVK GSP LINAYP+FAY+ NPK VSLDFVLFQPNQG  DPGSN HYDNMLFAQ+DAVY
Sbjct: 188 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 247

Query: 253 YALAAIG--YKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKK-GTPLRP 312
           +AL A+G  YKK+P+ +SETGWPS GD  E GAT +NA+KYNGNL+K +  KK  TP+RP
Sbjct: 248 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 307

Query: 313 SSDLNIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLGFSINDAVGNGTHGTSSAQPS 372
             DL I+VFALFNENMKPGPTSERNYGLF PDGTPVY LG   +    +G+  ++S   S
Sbjct: 308 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGS 367

Query: 373 PPG---------------PPTT---STGYLAISSATERSHRVGFFSACLFLMLFKLL 409
             G                P T   S  Y++ISSA  +     F    LF  L  ++
Sbjct: 368 SSGGGGNTGGSSSGGGIYQPVTGNPSPDYMSISSAGGKGR---FVECVLFFFLLCII 421

[Close]

BLAST of Lsi09G001300 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 421.8 bits (1083), Expect = 6.4e-118
Identity = 202/336 (60.12%), Postives = 260/336 (77.38%), Query Frame = 0


Query: 15  VLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLRAF 74
           VLLL+   L   +    VG+NYGQIANNLP+P  V  L++S+  T+VKLYDA P VL +F
Sbjct: 12  VLLLLFLTLSERIKGQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 71

Query: 75  ANTSVEFIVGLGNEYLAKMK-NPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTSL 134
           +N+ V+F++GLGNEYL  M  +P  A++W++  ++ +   T+ITSI VGNE+   ND  L
Sbjct: 72  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 131

Query: 135 TANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILDF 194
             +LLPAM+SV+ AL NLGL+KQV VT+AHSL IL TSYPPS+G F+ + +  L P+LDF
Sbjct: 132 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 191

Query: 195 HVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYY 254
           H +I SPFLINAYP+FAYK +PK+V L++VLFQPNQG++DP +NLHYDNMLFAQ+DA+Y 
Sbjct: 192 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 251

Query: 255 ALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDL 314
           A+  +G+  + V ISETGWPSKGDE+E GA+PENA  YNGNLLK I Q+KGTP + S  +
Sbjct: 252 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 311

Query: 315 NIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLG 350
           ++YVFALFNEN+KPGP SERNYGLF PDG PVY +G
Sbjct: 312 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVG 347

[Close]

BLAST of Lsi09G001300 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 421.8 bits (1083), Expect = 6.4e-118
Identity = 202/336 (60.12%), Postives = 260/336 (77.38%), Query Frame = 0


Query: 15  VLLLISGLLLLPVMVSSVGVNYGQIANNLPAPENVIPLVKSIGATKVKLYDASPKVLRAF 74
           VLLL+   L   +    VG+NYGQIANNLP+P  V  L++S+  T+VKLYDA P VL +F
Sbjct: 12  VLLLLFLTLSERIKGQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSF 71

Query: 75  ANTSVEFIVGLGNEYLAKMK-NPACAEEWVKNNVQAYFPGTKITSIFVGNEVLTFNDTSL 134
           +N+ V+F++GLGNEYL  M  +P  A++W++  ++ +   T+ITSI VGNE+   ND  L
Sbjct: 72  SNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVL 131

Query: 135 TANLLPAMQSVHTALVNLGLDKQVAVTTAHSLAILETSYPPSAGVFRRDLVDCLVPILDF 194
             +LLPAM+SV+ AL NLGL+KQV VT+AHSL IL TSYPPS+G F+ + +  L P+LDF
Sbjct: 132 IQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDF 191

Query: 195 HVKIGSPFLINAYPYFAYKANPKQVSLDFVLFQPNQGVLDPGSNLHYDNMLFAQIDAVYY 254
           H +I SPFLINAYP+FAYK +PK+V L++VLFQPNQG++DP +NLHYDNMLFAQ+DA+Y 
Sbjct: 192 HSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYS 251

Query: 255 ALAAIGYKKLPVHISETGWPSKGDEDEAGATPENAKKYNGNLLKFICQKKGTPLRPSSDL 314
           A+  +G+  + V ISETGWPSKGDE+E GA+PENA  YNGNLLK I Q+KGTP + S  +
Sbjct: 252 AIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPI 311

Query: 315 NIYVFALFNENMKPGPTSERNYGLFKPDGTPVYQLG 350
           ++YVFALFNEN+KPGP SERNYGLF PDG PVY +G
Sbjct: 312 DVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVG 347

The following BLAST results are available for this feature:

BLAST of Lsi09G001300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L868	1.5e-143	62.23	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9FHX5	6.6e-136	60.91	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9ZQG9	9.0e-117	60.12	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q8VYE5	3.8e-83	44.13	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q94G86	2.7e-81	44.74	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]

BLAST of Lsi09G001300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KK78	3.4e-228	97.79	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G146960 PE=3...	[more]
A0A5A7TM33	5.7e-223	96.09	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3ATQ5	5.7e-223	96.09	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482869 PE=3 SV...	[more]
A0A6J1H1N2	2.0e-220	93.43	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459311 P...	[more]
A0A6J1K8L2	3.2e-218	92.94	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491117 PE=...	[more]

BLAST of Lsi09G001300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654675.1	7.1e-228	97.79	glucan endo-1,3-beta-glucosidase 11 [Cucumis sativus] >KGN49973.1 hypothetical p...	[more]
XP_038906881.1	2.7e-227	97.55	glucan endo-1,3-beta-glucosidase 11 [Benincasa hispida]	[more]
XP_008437454.1	1.2e-222	96.09	PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo] >KAA0042641.1 gluc...	[more]
XP_023534433.1	4.9e-221	93.67	glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo]	[more]
KAG6605611.1	4.9e-221	93.67	Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of Lsi09G001300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32860.1	1.0e-144	62.23	Glycosyl hydrolase superfamily protein	[more]
AT5G42100.2	3.6e-137	61.35	beta-1,3-glucanase_putative	[more]
AT5G42100.1	4.7e-137	60.91	beta-1,3-glucanase_putative	[more]
AT2G27500.1	6.4e-118	60.12	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.2	6.4e-118	60.12	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 32..350 e-value: 6.0E-87 score: 292.2
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 30..357 e-value: 1.2E-123 score: 414.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 357..379
None	No IPR available	PANTHER	PTHR32227:SF348	GLYCOSIDE HYDROLASE FAMILY 17-RELATED	coord: 16..400
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 16..400
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 32..349

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G001300.1	Lsi09G001300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds