Lsi09G000320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi09G000320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionJ domain-containing protein
Locationchr09: 305164 .. 307485 (+)
RNA-Seq ExpressionLsi09G000320
SyntenyLsi09G000320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTGCAACAAAGACGAGGCAGCTAGAGCAAAAGAAATTGCCGAGAGAAAGTTTTCAGAAAGAAATTATTCTGCTGCAAAGAAGTTTGTTTTGAAGGCTCAAAATTTGTACCCTGGGCTTGATGGTCTCTCTCAAATGATGACAACTCTCGAAGTGTATATCTCCGCAGAGAATAAGATAAATGGGGAAACAGATTGGTATGGAATTCTCGGTGTGAACCACTTGGCTGATGACGAGACTATTAGGAAACAATATAGAAAACTGGCTCTTGTCCTTCATCCTGATAAAAACAAATCACTTGGTGCAGAGGGTGCATTTAAATTGGTTTCAGAGGCATGGAGTTTGTTATCTGACAAGGCAAAGAGGTTGGCTTATAATCAGAAGAGGGACCTGAAAGGAGGTCGACAGAAAACTCCTACCCACTCTCATAGTACTTCAGTGCCGCCAAGTGCAAATGGATTTCAAAATTTTAAGAATGTTGCTCCAAATGCAAGGAACGTTCAAACTAAGGTCCAGGCAGGGGCTACTACCCCATTACAGCCTTCTCTTAGAAAACCAGAAACCTTTTGGACTCTTTGCAATCGATGCAAGACACATTACGAGTACCTTAGGGTCTATCTAAATCACACTCTCCTTTGTCCCAATTGTCATGAAGCTTTTTTAGCTGTAGAGAAGGCTCCACCCCCAAATGTATTCAAGTCACCTAGTTGGTCTTCTCAGCAGCAGCATCAAAATTCTAGACAGCATCCTGTTAGTGGCAATGTCTATGGTACTGGAAGAAATGCTAAAAATTCTGATATTGGGCATTCTGTGGGTGTTAATTCAGTTGATAGTACAAACTTCCAGTGGGGTCCTTCCTCCAGGACAGCTGGTGTTGGTAGCAACTTCTCATCAGCTTCCGCGCAAGCTGCAAATTTGGTTCAACAGGCCAGTGAGAAAGTGAAGAGAGATCGTGATGAAACACAGTCATCAATTGAAGTGGAGAGGAGTCATTTGCCCTCTTCAAAGAAGAAAAGAACTGATGGAATTAATAACTATGGGGTCCACGTGGCAAACCAAATGGCCAGAGGAGATGGATCAGCTGGTGTTGGTTTACCTGAATTAAGGAAGAGTTATCCTGATACTCAAAAGTTTCATAGTTTTTATGGTGCATTTAACAGGACCAATAGCCAGAGGGAGTTGTCAATCTTTGAAATTCGAAACATGCTAATGGACAAGGCTCGAGCTGAAATATGTAAGAAACTTAAAGAATGGAGCTCATTGGCTGAAAAGTCAACTTTGAATAAACAATCAAAGAAGCAAAAGAGTGTGGTGAATGATGGAACTCATGATATAAAAATTAATGGTAAATCTTCTGCAAATGGTAAAAGAGGGCACGAGAGGAAGCCTGAGTCAGACTCTATAGCTGGTAAAAATACAGGCAGTGAGAATGATCCCATCTCAATTAATGTTCCAGATCCTGATTTCCATAATTTTGACTTGGACCGAGCTGAAAGTTCATTTGGGGATGATCAAGTCTGGGCATGTTATGATGATGATGATGGAATGCCTCGTTTCTATGCTCGAATTCACAAGGTGATTTCCCTAAGACCATTCAGAATGCGTATCAGTTGGCTCAATTCAAGAAGCAACACTGAGATTGGCCCGATGGACTGGATAGGTTCAGGGTTCACAAAAACCTGTGGGGATTTCAGAATTGGGAGACATGAAGTCACAAGATCATTGAACTCTTTCTCGCATAAGGTTTGCTGGGTAAAAGGTTTACGAGGTGTGATTCGGATATTTCCTCAGAAGGGTGAAGTCTGGGCTCTTTATAGAAACTGGTCTGCAGATTGGAATAAAAATACTTCAGAAGAAATGATACACAAGTATGATATGGTAGAAGTGCTTGATGATTTTAATGAAGAACAAGGTGTGTCTGTTACTCCTCTTGTTAAGGAGGTTGGTTTCAGGACAGTGTTCCGTACACACATGGACCCAAAAGAAGTGAGGAAGATCCCTAAAGAAGAGATGTTTCGCTTCTCCCATCAGGTCCCCAATTACTTACTTACGGGAGAAGAAGCTCAAAATGCTCCAAAGGGTTGTCGGGAGTTGGATCCAGCAGCAACACCTTTGGAGCTCCTCCAGATTGATACAGAATTGAAACGGGCAACGGCAAAGGAGACTGAGTTAAAAACTGAAGAAGCAATATCTCATATTAACCAGGAAACTGTAGTTAATGAGGTGGAAGACACTTCGGAAGCTAGGAAGGTAGATGTAGTAACCCAGGAAGATGAACAAACTAAGATGTCTGGTGATGTTGCCTTCCAGGGTAGCTAA

mRNA sequence

ATGGAGTGCAACAAAGACGAGGCAGCTAGAGCAAAAGAAATTGCCGAGAGAAAGTTTTCAGAAAGAAATTATTCTGCTGCAAAGAAGTTTGTTTTGAAGGCTCAAAATTTGTACCCTGGGCTTGATGGTCTCTCTCAAATGATGACAACTCTCGAAGTGTATATCTCCGCAGAGAATAAGATAAATGGGGAAACAGATTGGTATGGAATTCTCGGTGTGAACCACTTGGCTGATGACGAGACTATTAGGAAACAATATAGAAAACTGGCTCTTGTCCTTCATCCTGATAAAAACAAATCACTTGGTGCAGAGGGTGCATTTAAATTGGTTTCAGAGGCATGGAGTTTGTTATCTGACAAGGCAAAGAGGTTGGCTTATAATCAGAAGAGGGACCTGAAAGGAGGTCGACAGAAAACTCCTACCCACTCTCATAGTACTTCAGTGCCGCCAAGTGCAAATGGATTTCAAAATTTTAAGAATGTTGCTCCAAATGCAAGGAACGTTCAAACTAAGGTCCAGGCAGGGGCTACTACCCCATTACAGCCTTCTCTTAGAAAACCAGAAACCTTTTGGACTCTTTGCAATCGATGCAAGACACATTACGAGTACCTTAGGGTCTATCTAAATCACACTCTCCTTTGTCCCAATTGTCATGAAGCTTTTTTAGCTGTAGAGAAGGCTCCACCCCCAAATGTATTCAAGTCACCTAGTTGGTCTTCTCAGCAGCAGCATCAAAATTCTAGACAGCATCCTGTTAGTGGCAATGTCTATGGTACTGGAAGAAATGCTAAAAATTCTGATATTGGGCATTCTGTGGGTGTTAATTCAGTTGATAGTACAAACTTCCAGTGGGGTCCTTCCTCCAGGACAGCTGGTGTTGGTAGCAACTTCTCATCAGCTTCCGCGCAAGCTGCAAATTTGGTTCAACAGGCCAGTGAGAAAGTGAAGAGAGATCGTGATGAAACACAGTCATCAATTGAAGTGGAGAGGAGTCATTTGCCCTCTTCAAAGAAGAAAAGAACTGATGGAATTAATAACTATGGGGTCCACGTGGCAAACCAAATGGCCAGAGGAGATGGATCAGCTGGTGTTGGTTTACCTGAATTAAGGAAGAGTTATCCTGATACTCAAAAGTTTCATAGTTTTTATGGTGCATTTAACAGGACCAATAGCCAGAGGGAGTTGTCAATCTTTGAAATTCGAAACATGCTAATGGACAAGGCTCGAGCTGAAATATGTAAGAAACTTAAAGAATGGAGCTCATTGGCTGAAAAGTCAACTTTGAATAAACAATCAAAGAAGCAAAAGAGTGTGGTGAATGATGGAACTCATGATATAAAAATTAATGGTAAATCTTCTGCAAATGGTAAAAGAGGGCACGAGAGGAAGCCTGAGTCAGACTCTATAGCTGGTAAAAATACAGGCAGTGAGAATGATCCCATCTCAATTAATGTTCCAGATCCTGATTTCCATAATTTTGACTTGGACCGAGCTGAAAGTTCATTTGGGGATGATCAAGTCTGGGCATGTTATGATGATGATGATGGAATGCCTCGTTTCTATGCTCGAATTCACAAGGTGATTTCCCTAAGACCATTCAGAATGCGTATCAGTTGGCTCAATTCAAGAAGCAACACTGAGATTGGCCCGATGGACTGGATAGGTTCAGGGTTCACAAAAACCTGTGGGGATTTCAGAATTGGGAGACATGAAGTCACAAGATCATTGAACTCTTTCTCGCATAAGGTTTGCTGGGTAAAAGGTTTACGAGGTGTGATTCGGATATTTCCTCAGAAGGGTGAAGTCTGGGCTCTTTATAGAAACTGGTCTGCAGATTGGAATAAAAATACTTCAGAAGAAATGATACACAAGTATGATATGGTAGAAGTGCTTGATGATTTTAATGAAGAACAAGGTGTGTCTGTTACTCCTCTTGTTAAGGAGGTTGGTTTCAGGACAGTGTTCCGTACACACATGGACCCAAAAGAAGTGAGGAAGATCCCTAAAGAAGAGATGTTTCGCTTCTCCCATCAGGTCCCCAATTACTTACTTACGGGAGAAGAAGCTCAAAATGCTCCAAAGGGTTGTCGGGAGTTGGATCCAGCAGCAACACCTTTGGAGCTCCTCCAGATTGATACAGAATTGAAACGGGCAACGGCAAAGGAGACTGAGTTAAAAACTGAAGAAGCAATATCTCATATTAACCAGGAAACTGTAGTTAATGAGGTGGAAGACACTTCGGAAGCTAGGAAGGTAGATGTAGTAACCCAGGAAGATGAACAAACTAAGATGTCTGGTGATGTTGCCTTCCAGGGTAGCTAA

Coding sequence (CDS)

ATGGAGTGCAACAAAGACGAGGCAGCTAGAGCAAAAGAAATTGCCGAGAGAAAGTTTTCAGAAAGAAATTATTCTGCTGCAAAGAAGTTTGTTTTGAAGGCTCAAAATTTGTACCCTGGGCTTGATGGTCTCTCTCAAATGATGACAACTCTCGAAGTGTATATCTCCGCAGAGAATAAGATAAATGGGGAAACAGATTGGTATGGAATTCTCGGTGTGAACCACTTGGCTGATGACGAGACTATTAGGAAACAATATAGAAAACTGGCTCTTGTCCTTCATCCTGATAAAAACAAATCACTTGGTGCAGAGGGTGCATTTAAATTGGTTTCAGAGGCATGGAGTTTGTTATCTGACAAGGCAAAGAGGTTGGCTTATAATCAGAAGAGGGACCTGAAAGGAGGTCGACAGAAAACTCCTACCCACTCTCATAGTACTTCAGTGCCGCCAAGTGCAAATGGATTTCAAAATTTTAAGAATGTTGCTCCAAATGCAAGGAACGTTCAAACTAAGGTCCAGGCAGGGGCTACTACCCCATTACAGCCTTCTCTTAGAAAACCAGAAACCTTTTGGACTCTTTGCAATCGATGCAAGACACATTACGAGTACCTTAGGGTCTATCTAAATCACACTCTCCTTTGTCCCAATTGTCATGAAGCTTTTTTAGCTGTAGAGAAGGCTCCACCCCCAAATGTATTCAAGTCACCTAGTTGGTCTTCTCAGCAGCAGCATCAAAATTCTAGACAGCATCCTGTTAGTGGCAATGTCTATGGTACTGGAAGAAATGCTAAAAATTCTGATATTGGGCATTCTGTGGGTGTTAATTCAGTTGATAGTACAAACTTCCAGTGGGGTCCTTCCTCCAGGACAGCTGGTGTTGGTAGCAACTTCTCATCAGCTTCCGCGCAAGCTGCAAATTTGGTTCAACAGGCCAGTGAGAAAGTGAAGAGAGATCGTGATGAAACACAGTCATCAATTGAAGTGGAGAGGAGTCATTTGCCCTCTTCAAAGAAGAAAAGAACTGATGGAATTAATAACTATGGGGTCCACGTGGCAAACCAAATGGCCAGAGGAGATGGATCAGCTGGTGTTGGTTTACCTGAATTAAGGAAGAGTTATCCTGATACTCAAAAGTTTCATAGTTTTTATGGTGCATTTAACAGGACCAATAGCCAGAGGGAGTTGTCAATCTTTGAAATTCGAAACATGCTAATGGACAAGGCTCGAGCTGAAATATGTAAGAAACTTAAAGAATGGAGCTCATTGGCTGAAAAGTCAACTTTGAATAAACAATCAAAGAAGCAAAAGAGTGTGGTGAATGATGGAACTCATGATATAAAAATTAATGGTAAATCTTCTGCAAATGGTAAAAGAGGGCACGAGAGGAAGCCTGAGTCAGACTCTATAGCTGGTAAAAATACAGGCAGTGAGAATGATCCCATCTCAATTAATGTTCCAGATCCTGATTTCCATAATTTTGACTTGGACCGAGCTGAAAGTTCATTTGGGGATGATCAAGTCTGGGCATGTTATGATGATGATGATGGAATGCCTCGTTTCTATGCTCGAATTCACAAGGTGATTTCCCTAAGACCATTCAGAATGCGTATCAGTTGGCTCAATTCAAGAAGCAACACTGAGATTGGCCCGATGGACTGGATAGGTTCAGGGTTCACAAAAACCTGTGGGGATTTCAGAATTGGGAGACATGAAGTCACAAGATCATTGAACTCTTTCTCGCATAAGGTTTGCTGGGTAAAAGGTTTACGAGGTGTGATTCGGATATTTCCTCAGAAGGGTGAAGTCTGGGCTCTTTATAGAAACTGGTCTGCAGATTGGAATAAAAATACTTCAGAAGAAATGATACACAAGTATGATATGGTAGAAGTGCTTGATGATTTTAATGAAGAACAAGGTGTGTCTGTTACTCCTCTTGTTAAGGAGGTTGGTTTCAGGACAGTGTTCCGTACACACATGGACCCAAAAGAAGTGAGGAAGATCCCTAAAGAAGAGATGTTTCGCTTCTCCCATCAGGTCCCCAATTACTTACTTACGGGAGAAGAAGCTCAAAATGCTCCAAAGGGTTGTCGGGAGTTGGATCCAGCAGCAACACCTTTGGAGCTCCTCCAGATTGATACAGAATTGAAACGGGCAACGGCAAAGGAGACTGAGTTAAAAACTGAAGAAGCAATATCTCATATTAACCAGGAAACTGTAGTTAATGAGGTGGAAGACACTTCGGAAGCTAGGAAGGTAGATGTAGTAACCCAGGAAGATGAACAAACTAAGATGTCTGGTGATGTTGCCTTCCAGGGTAGCTAA

Protein sequence

MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENKINGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDKAKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPLQPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSSQQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSSASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGDGSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEWSSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSENDPISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKGEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHMDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRATAKETELKTEEAISHINQETVVNEVEDTSEARKVDVVTQEDEQTKMSGDVAFQGS
Homology
BLAST of Lsi09G000320 vs. ExPASy Swiss-Prot
Match: Q149L6 (DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.0e-13
Identity = 59/187 (31.55%), Postives = 83/187 (44.39%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYP--------------------- 60
           ME N+DEA +  +IA    S  N   A++F+ KA+ LYP                     
Sbjct: 1   MEGNRDEAEKCVQIAREALSAGNRDKAQRFLQKAEKLYPLPAARALLEIIMKNGSTAGSS 60

Query: 61  -----------------GLDGLSQMMTTLEVY----ISAENKINGETDWYGILGVNHLAD 120
                            G DG S      +VY    +     IN   ++Y +LGV   A 
Sbjct: 61  THCRKPPGSSDQSKPSCGKDGTSGAGEGGKVYTKDQVEGVLSINKCKNYYEVLGVTKDAG 120

Query: 121 DETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDKAKRLAYNQKRDLKGGRQK 146
           DE ++K YRKLAL  HPDKN + GA  AFK +  A+++LS+  KR  Y    DL G  ++
Sbjct: 121 DEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQY----DLTGSEEQ 180

BLAST of Lsi09G000320 vs. ExPASy Swiss-Prot
Match: Q9QYI4 (DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 7.8e-13
Identity = 62/190 (32.63%), Postives = 83/190 (43.68%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPG------LDGLSQM------- 60
           ME NKDEA R   IA +         A +F+ KAQ LYP       ++ L+Q        
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESLNQKPQSTGDH 60

Query: 61  --------MTTLEVYISAENKINGET------------------------DWYGILGVNH 120
                    TT +   +     NGE                         D+Y ILGV+ 
Sbjct: 61  PQPTDTTHTTTKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILGVSR 120

Query: 121 LADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDKAKRLAYNQKRDLKGG 146
            A DE ++K YRKLAL  HPDKN + GA  AFK +  A+++LS+  KR  Y+Q  D    
Sbjct: 121 SASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGD---D 180

BLAST of Lsi09G000320 vs. ExPASy Swiss-Prot
Match: Q58DR2 (DnaJ homolog subfamily B member 12 OS=Bos taurus OX=9913 GN=DNAJB12 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-12
Identity = 63/188 (33.51%), Postives = 84/188 (44.68%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPG------LDGLSQM------- 60
           ME NKDEA R   IA +         A +F+ KAQ LYP       ++ L+Q        
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVHALIESLNQKPQPAGDQ 60

Query: 61  -MTTLEVY-----------ISAENKINGET-------------------DWYGILGVNHL 120
              T   +            SA  +  GE+                   D+Y ILGV+  
Sbjct: 61  PQPTEATHTTHRKAAGANTASANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRG 120

Query: 121 ADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDKAKRLAYNQKRDLKGGR 145
           A DE ++K YRKLAL  HPDKN + GA  AFK +  A+++LS+  KR  Y+Q  D KG  
Sbjct: 121 ASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKG-- 180

BLAST of Lsi09G000320 vs. ExPASy Swiss-Prot
Match: Q9NXW2 (DnaJ homolog subfamily B member 12 OS=Homo sapiens OX=9606 GN=DNAJB12 PE=1 SV=5)

HSP 1 Score: 73.6 bits (179), Expect = 1.1e-11
Identity = 57/188 (30.32%), Postives = 81/188 (43.09%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPG------LDGLSQMMTT---- 60
           ME NKDEA R   IA +         A +F+ KAQ LYP       ++ L+Q   T    
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQ 60

Query: 61  ----------------------------------LEVYISAENKINGETDWYGILGVNHL 120
                                                 ++A  ++    D+Y ILGV+  
Sbjct: 61  PPPTDTTHATHRKAGGTDAPSANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRG 120

Query: 121 ADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDKAKRLAYNQKRDLKGGR 145
           A DE ++K YR+LAL  HPDKN + GA  AFK +  A+++LS+  KR  Y+Q  D    +
Sbjct: 121 ASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGD---DK 180

BLAST of Lsi09G000320 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-11
Identity = 54/161 (33.54%), Postives = 76/161 (47.20%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGL------------------- 60
           M+ NKD+A+R   IAE      +   A KF+  A+ L P L                   
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSLSVDELVAACDNLDSVSRNS 60

Query: 61  ---------DGLSQMMTTLEVYISAEN-----KINGETDWYGILGVNHLADDETIRKQYR 120
                    DG    + T ++  + EN      I    D+Y ILG+      + IRK YR
Sbjct: 61  SVSEKLKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDEIRKAYR 120

Query: 121 KLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDKAKRLAYNQ 129
           KL+L +HPDKNK+ G+E AFK VS+A++ LSD   R  ++Q
Sbjct: 121 KLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFDQ 161

BLAST of Lsi09G000320 vs. ExPASy TrEMBL
Match: A0A5D3C403 (Curved DNA-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00560 PE=4 SV=1)

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 694/753 (92.16%), Postives = 714/753 (94.82%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTHSHSTS PPSANGFQNFKN APNARNVQTKVQ G TTP 
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSAPPSANGFQNFKNAAPNARNVQTKVQVGPTTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 -QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSS 300
            QQQHQNSRQH VSGN YGTGRNAKN D GHSVGVNSVD+TNF W PSSR  G GSNFSS
Sbjct: 241 QQQQHQNSRQHSVSGNTYGTGRNAKNPDTGHSVGVNSVDNTNFHWSPSSRPTGTGSNFSS 300

Query: 301 ASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGD 360
           ASAQAANLVQQASEKVKRDRDETQ+S+EVERSHL SSKKKRTDGINNYGVHVANQMARGD
Sbjct: 301 ASAQAANLVQQASEKVKRDRDETQASLEVERSHLTSSKKKRTDGINNYGVHVANQMARGD 360

Query: 361 GSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEW 420
           GSAG GLPE RKSY DTQKFHSFYGAFN  NSQRELSIFEIRNMLMDKARAEI KKLKEW
Sbjct: 361 GSAGDGLPETRKSYSDTQKFHSFYGAFNNNNSQRELSIFEIRNMLMDKARAEIRKKLKEW 420

Query: 421 SSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSEND 480
            S+AEK+TLNKQSKKQKSV+NDGT DIKINGKSSANGK  H RKPESDS+AGKNTGS ND
Sbjct: 421 RSMAEKATLNKQSKKQKSVLNDGTPDIKINGKSSANGKGWHGRKPESDSLAGKNTGSAND 480

Query: 481 PISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISW 540
           PI+INVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISL+PFRMRISW
Sbjct: 481 PITINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLKPFRMRISW 540

Query: 541 LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600
           LNSRSNTEIGPMDW+GSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK
Sbjct: 541 LNSRSNTEIGPMDWVGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600

Query: 601 GEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHM 660
           GEVWALYRNWS DWNK+TSEEM+HKYDMVEVLDDFNEEQGVSV PLVK +GFRTVFRT+M
Sbjct: 601 GEVWALYRNWSVDWNKDTSEEMVHKYDMVEVLDDFNEEQGVSVAPLVKVIGFRTVFRTNM 660

Query: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRAT 720
           DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQID E  +AT
Sbjct: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDAESNQAT 720

Query: 721 AKETELKTEEAISHINQETVVNEVEDTSEARKV 753
            KET++KTEEAIS IN+E VV EVEDT EAR+V
Sbjct: 721 TKETQVKTEEAISRINEENVVYEVEDTLEARRV 753

BLAST of Lsi09G000320 vs. ExPASy TrEMBL
Match: A0A0A0KJH0 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139530 PE=4 SV=1)

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 693/755 (91.79%), Postives = 713/755 (94.44%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADD+TIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDDTIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTHSHSTS P SANGFQNFKN APNARNVQTKVQ G TTP 
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSAPASANGFQNFKNAAPNARNVQTKVQVGPTTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 --QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFS 300
             QQQHQNSRQHPVS N YGTGRNAKN D GHSVGVNSVD+TNF WGPSSRT G GSNFS
Sbjct: 241 QQQQQHQNSRQHPVSSNTYGTGRNAKNPDTGHSVGVNSVDNTNFHWGPSSRTTGTGSNFS 300

Query: 301 SASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARG 360
           SASAQAAN VQQASEKVKRDRDETQ+S+EVERSHL SSKKKRTDGINN+GVHVANQ+ RG
Sbjct: 301 SASAQAANFVQQASEKVKRDRDETQASLEVERSHLTSSKKKRTDGINNFGVHVANQIVRG 360

Query: 361 DGSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKE 420
           DGSAG GLPE RKSY D+QKFHSFYGAFNR NSQRELSIFEIRNMLMDKARAEI KKLKE
Sbjct: 361 DGSAGDGLPESRKSYSDSQKFHSFYGAFNRNNSQRELSIFEIRNMLMDKARAEIRKKLKE 420

Query: 421 WSSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSEN 480
           W S+AEK+TLNKQSKKQKSV+NDGTHDIKINGKSSANGK  H RKPESDS+AGKNTGS  
Sbjct: 421 WRSMAEKATLNKQSKKQKSVLNDGTHDIKINGKSSANGKGWHGRKPESDSLAGKNTGSAK 480

Query: 481 DPISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRIS 540
           DPI+INVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVIS +PFRMRIS
Sbjct: 481 DPITINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISRKPFRMRIS 540

Query: 541 WLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQ 600
           WLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCW KGLRGVIRIFPQ
Sbjct: 541 WLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWAKGLRGVIRIFPQ 600

Query: 601 KGEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTH 660
           KGEVWALYRNWS DWNK+TSEEM+HKYDMVEVLDDFNEEQGVSV PLVK +GFRTVFRTH
Sbjct: 601 KGEVWALYRNWSVDWNKDTSEEMVHKYDMVEVLDDFNEEQGVSVAPLVKVIGFRTVFRTH 660

Query: 661 MDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRA 720
           MDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQID E  +A
Sbjct: 661 MDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDAESNQA 720

Query: 721 TAKETELKTEEAISHINQETVVNEVEDTSEARKVD 754
           T KET +KTEEAIS IN+E VVNEVEDT EARKVD
Sbjct: 721 TTKETRVKTEEAISCINEENVVNEVEDTLEARKVD 755

BLAST of Lsi09G000320 vs. ExPASy TrEMBL
Match: A0A1S3AV00 (uncharacterized protein LOC103482955 OS=Cucumis melo OX=3656 GN=LOC103482955 PE=4 SV=1)

HSP 1 Score: 1386.3 bits (3587), Expect = 0.0e+00
Identity = 694/752 (92.29%), Postives = 712/752 (94.68%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTHSHSTS PPSANGFQNFKN APNARNVQTKVQ G TTP 
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSAPPSANGFQNFKNAAPNARNVQTKVQVGPTTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 -QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSS 300
            QQQHQNSRQH VSGN YGTGRNAKN D GHSVGVNSVD+TNF W PSSR  G GSNFSS
Sbjct: 241 QQQQHQNSRQHSVSGNTYGTGRNAKNPDTGHSVGVNSVDNTNFHWSPSSRPTGTGSNFSS 300

Query: 301 ASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGD 360
           ASAQAANLVQQASEKVKRDRDETQ S+EVERSHL SSKKKRTDGINNYGVHVANQMARGD
Sbjct: 301 ASAQAANLVQQASEKVKRDRDETQPSLEVERSHLTSSKKKRTDGINNYGVHVANQMARGD 360

Query: 361 GSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEW 420
           GSAG GLPE RKSY DTQKFHSFYGAFN  NSQRELSIFEIRNMLMDKARAEI KKLKEW
Sbjct: 361 GSAGDGLPETRKSYSDTQKFHSFYGAFNNNNSQRELSIFEIRNMLMDKARAEIRKKLKEW 420

Query: 421 SSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSEND 480
            S+AEK+TLNKQSKKQKSV+NDGT DIKINGKSSANGK  H RKPESDS+AGKNTGS ND
Sbjct: 421 RSMAEKATLNKQSKKQKSVLNDGTPDIKINGKSSANGKGWHGRKPESDSLAGKNTGSAND 480

Query: 481 PISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISW 540
           PI+INVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISL+PFRMRISW
Sbjct: 481 PITINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLKPFRMRISW 540

Query: 541 LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600
           LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK
Sbjct: 541 LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600

Query: 601 GEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHM 660
           GEVWALYRNWS DWNK+TSEEM+HKYDMVEVLDDFNEEQGVSV PLVK +GFRTVFRT+M
Sbjct: 601 GEVWALYRNWSVDWNKDTSEEMVHKYDMVEVLDDFNEEQGVSVAPLVKVIGFRTVFRTNM 660

Query: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRAT 720
           DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQID E  +AT
Sbjct: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDAESNQAT 720

Query: 721 AKETELKTEEAISHINQETVVNEVEDTSEARK 752
            KET++KTEEAIS IN+E VV EVEDT EAR+
Sbjct: 721 TKETQVKTEEAISRINEENVVYEVEDTLEARR 752

BLAST of Lsi09G000320 vs. ExPASy TrEMBL
Match: A0A6J1I7J9 (uncharacterized protein LOC111471541 OS=Cucurbita maxima OX=3661 GN=LOC111471541 PE=4 SV=1)

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 695/773 (89.91%), Postives = 728/773 (94.18%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGE DWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGEMDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTH+HSTSVPPSANGFQ+FKNVA NARNVQ+KV  GATTPL
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHTHSTSVPPSANGFQSFKNVASNARNVQSKVHVGATTPL 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPS RKP+TFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVE+APPPNV KSPSWSS
Sbjct: 181 QPSQRKPDTFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVERAPPPNVLKSPSWSS 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSSA 300
           QQQHQNSRQ+  +G+ YGTGRN+K  D GHSVG+NSVD+TNFQWGPSSRTAGVGS FSSA
Sbjct: 241 QQQHQNSRQY-TTGDAYGTGRNSKKPDSGHSVGINSVDNTNFQWGPSSRTAGVGSTFSSA 300

Query: 301 SAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGDG 360
           SA+AANL QQASEKVKRDRDETQ S EV+RS L SSKKKRTDGIN++GVH ANQMA GDG
Sbjct: 301 SARAANLGQQASEKVKRDRDETQPSTEVDRSQL-SSKKKRTDGINSFGVHAANQMASGDG 360

Query: 361 SAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEWS 420
           SAGVGL ELRKSYPDTQKFHSFY AFNR +SQRELSIFEIRNMLMDK+RAEI KKLKEWS
Sbjct: 361 SAGVGLSELRKSYPDTQKFHSFYYAFNRIDSQRELSIFEIRNMLMDKSRAEIHKKLKEWS 420

Query: 421 SLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSENDP 480
           SLAEKSTLNKQSKKQK+VVNDGTHDIKINGK SANGK  +E++PESDS+A KNTGSENDP
Sbjct: 421 SLAEKSTLNKQSKKQKNVVNDGTHDIKINGKPSANGKGWNEKEPESDSLASKNTGSENDP 480

Query: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWL 540
           ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKV+SLRPFRMR SWL
Sbjct: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVMSLRPFRMRFSWL 540

Query: 541 NSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKG 600
           NSRSN EIGPMDWIGSGFTKTCGDFRIG+HEVTRSLNSFSHKVCWVKGLRGVI+IFPQKG
Sbjct: 541 NSRSNMEIGPMDWIGSGFTKTCGDFRIGKHEVTRSLNSFSHKVCWVKGLRGVIQIFPQKG 600

Query: 601 EVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHMD 660
           EVWALYRNWSADWNK+TSEEMIHKYDMVEVLDDFNE++GVSV PLVK  GFRTVF  +M 
Sbjct: 601 EVWALYRNWSADWNKDTSEEMIHKYDMVEVLDDFNEKEGVSVAPLVKVGGFRTVFCRNMH 660

Query: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRATA 720
           PKEVRKIPKEEMFRFSHQVPNYLLTG+EAQNAPKGCRELDPAATPLELLQIDTE  + T 
Sbjct: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGDEAQNAPKGCRELDPAATPLELLQIDTESVQVTT 720

Query: 721 KETELKTEEAISHINQETVVNEVEDTSEARKVDVVTQEDEQTKMSGDVAFQGS 774
           KETE+KT   ISHINQETVVNEVEDT EARKVDVVTQEDEQTKMSGDV FQGS
Sbjct: 721 KETEVKTVGEISHINQETVVNEVEDTLEARKVDVVTQEDEQTKMSGDVTFQGS 771

BLAST of Lsi09G000320 vs. ExPASy TrEMBL
Match: A0A6J1E363 (uncharacterized protein LOC111430376 OS=Cucurbita moschata OX=3662 GN=LOC111430376 PE=4 SV=1)

HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 691/773 (89.39%), Postives = 725/773 (93.79%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTH+HSTSVPPSANGFQNFKNVA NARNVQ+KVQ GA    
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHTHSTSVPPSANGFQNFKNVASNARNVQSKVQVGA---- 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
             S RKP+TFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVE+APPPNV KSPSWSS
Sbjct: 181 --SQRKPDTFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVERAPPPNVLKSPSWSS 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSSA 300
           QQQHQNSRQ+  +G+ YGTGRN+K  D GHS G+NSVD+TNFQWGPSSRTAGVGS FSSA
Sbjct: 241 QQQHQNSRQY-TTGDAYGTGRNSKKPDSGHSGGINSVDNTNFQWGPSSRTAGVGSTFSSA 300

Query: 301 SAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGDG 360
           SA+AANL QQASEKVKRDRDETQ S EV+RS L SSKKKRTDGIN++GVH ANQMA GDG
Sbjct: 301 SARAANLGQQASEKVKRDRDETQPSTEVDRSQL-SSKKKRTDGINSFGVHAANQMASGDG 360

Query: 361 SAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEWS 420
           SAGVGL E+RKSYPDTQKFHSFY AFNRT+SQRELSIFEIRNMLMDK+RAEI KKLKEWS
Sbjct: 361 SAGVGLSEVRKSYPDTQKFHSFYYAFNRTDSQRELSIFEIRNMLMDKSRAEIHKKLKEWS 420

Query: 421 SLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSENDP 480
           SLAEKSTLNKQSKKQK+VVNDGTHDIKINGK SANGK  +E++PESDS+A KNTGSENDP
Sbjct: 421 SLAEKSTLNKQSKKQKNVVNDGTHDIKINGKPSANGKGWNEKEPESDSLASKNTGSENDP 480

Query: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWL 540
           ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKV+SLRPFRMR SWL
Sbjct: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVMSLRPFRMRFSWL 540

Query: 541 NSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKG 600
           NSRSN EIGPMDW+GSGFTKTCGDFRIG+HEVTRSLNSFSHKVCWVKGLRGVI+IFPQKG
Sbjct: 541 NSRSNMEIGPMDWVGSGFTKTCGDFRIGKHEVTRSLNSFSHKVCWVKGLRGVIQIFPQKG 600

Query: 601 EVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHMD 660
           EVWALYRNWSADWNK+TSEEMIHKYDMVEVLDDFNE++GVSV PLVK  GFRTVF  +MD
Sbjct: 601 EVWALYRNWSADWNKDTSEEMIHKYDMVEVLDDFNEKEGVSVAPLVKVGGFRTVFCRNMD 660

Query: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRATA 720
           PKEVRKIPKEEMFRFSHQVPNYLLTG+EAQNAPKGCRELDPAATPLELLQIDTE  + T 
Sbjct: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGDEAQNAPKGCRELDPAATPLELLQIDTESFQVTT 720

Query: 721 KETELKTEEAISHINQETVVNEVEDTSEARKVDVVTQEDEQTKMSGDVAFQGS 774
           KETE+KT   ISHINQETVVNEVEDT EARKVDVVTQEDEQTKMSGDV FQGS
Sbjct: 721 KETEVKTVGEISHINQETVVNEVEDTLEARKVDVVTQEDEQTKMSGDVTFQGS 765

BLAST of Lsi09G000320 vs. NCBI nr
Match: XP_038874482.1 (uncharacterized protein LOC120067126 [Benincasa hispida])

HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 727/773 (94.05%), Postives = 741/773 (95.86%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISA+NK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISADNK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGE DWYGILGVNHLADDETIR+QYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGEMDWYGILGVNHLADDETIRRQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRD KGGRQKTPTHSHSTS PPSANGFQNFKNVAPNARNVQTKVQ GATTP 
Sbjct: 121 AKRLAYNQKRDPKGGRQKTPTHSHSTSAPPSANGFQNFKNVAPNARNVQTKVQVGATTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKP+TFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPDTFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSSA 300
           QQQHQNSRQHPVSGNVY TGRN KN D GHSVGVNSVD+TNFQWGPSSRT GVGSNFSSA
Sbjct: 241 QQQHQNSRQHPVSGNVYNTGRNVKNPDTGHSVGVNSVDNTNFQWGPSSRTTGVGSNFSSA 300

Query: 301 SAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGDG 360
           SAQAANLVQQASEKVKRDRDETQ SIEVERSHLPS KKK+TDGINNYGVHVANQ+ARGDG
Sbjct: 301 SAQAANLVQQASEKVKRDRDETQPSIEVERSHLPSLKKKKTDGINNYGVHVANQVARGDG 360

Query: 361 SAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEWS 420
           SAGVGL ELRKSYPDTQK HS YG FNR NSQRELSIFEIRNMLMDKARAEI KKLKEW 
Sbjct: 361 SAGVGLTELRKSYPDTQKLHSLYGVFNRNNSQRELSIFEIRNMLMDKARAEIRKKLKEWR 420

Query: 421 SLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSENDP 480
           SLAEKSTLNKQSKK+KSVVNDGTHDIKINGKSSANGK GHERKPESDS+AGK+T +ENDP
Sbjct: 421 SLAEKSTLNKQSKKKKSVVNDGTHDIKINGKSSANGKGGHERKPESDSLAGKSTDNENDP 480

Query: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWL 540
           ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWL
Sbjct: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWL 540

Query: 541 NSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKG 600
           NSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKG
Sbjct: 541 NSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKG 600

Query: 601 EVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHMD 660
           EVWALYRNWSADWNK TSEEMIHKYDMVEVL+DFNEE GVSV PLVK  GFRTVFRTHMD
Sbjct: 601 EVWALYRNWSADWNKGTSEEMIHKYDMVEVLNDFNEE-GVSVAPLVKVAGFRTVFRTHMD 660

Query: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRATA 720
           PKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTE  RA  
Sbjct: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTESNRAMT 720

Query: 721 KETELKTEEAISHINQETVVNEVEDTSEARKVDVVTQEDEQTKMSGDVAFQGS 774
           KETE+KTEEAISHINQETVVN+VEDT EARKVDVVTQEDE TKMSGDVAFQGS
Sbjct: 721 KETEVKTEEAISHINQETVVNQVEDTLEARKVDVVTQEDEHTKMSGDVAFQGS 772

BLAST of Lsi09G000320 vs. NCBI nr
Match: KAA0042558.1 (Curved DNA-binding protein [Cucumis melo var. makuwa] >TYK05962.1 Curved DNA-binding protein [Cucumis melo var. makuwa])

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 694/753 (92.16%), Postives = 714/753 (94.82%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTHSHSTS PPSANGFQNFKN APNARNVQTKVQ G TTP 
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSAPPSANGFQNFKNAAPNARNVQTKVQVGPTTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 -QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSS 300
            QQQHQNSRQH VSGN YGTGRNAKN D GHSVGVNSVD+TNF W PSSR  G GSNFSS
Sbjct: 241 QQQQHQNSRQHSVSGNTYGTGRNAKNPDTGHSVGVNSVDNTNFHWSPSSRPTGTGSNFSS 300

Query: 301 ASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGD 360
           ASAQAANLVQQASEKVKRDRDETQ+S+EVERSHL SSKKKRTDGINNYGVHVANQMARGD
Sbjct: 301 ASAQAANLVQQASEKVKRDRDETQASLEVERSHLTSSKKKRTDGINNYGVHVANQMARGD 360

Query: 361 GSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEW 420
           GSAG GLPE RKSY DTQKFHSFYGAFN  NSQRELSIFEIRNMLMDKARAEI KKLKEW
Sbjct: 361 GSAGDGLPETRKSYSDTQKFHSFYGAFNNNNSQRELSIFEIRNMLMDKARAEIRKKLKEW 420

Query: 421 SSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSEND 480
            S+AEK+TLNKQSKKQKSV+NDGT DIKINGKSSANGK  H RKPESDS+AGKNTGS ND
Sbjct: 421 RSMAEKATLNKQSKKQKSVLNDGTPDIKINGKSSANGKGWHGRKPESDSLAGKNTGSAND 480

Query: 481 PISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISW 540
           PI+INVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISL+PFRMRISW
Sbjct: 481 PITINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLKPFRMRISW 540

Query: 541 LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600
           LNSRSNTEIGPMDW+GSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK
Sbjct: 541 LNSRSNTEIGPMDWVGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600

Query: 601 GEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHM 660
           GEVWALYRNWS DWNK+TSEEM+HKYDMVEVLDDFNEEQGVSV PLVK +GFRTVFRT+M
Sbjct: 601 GEVWALYRNWSVDWNKDTSEEMVHKYDMVEVLDDFNEEQGVSVAPLVKVIGFRTVFRTNM 660

Query: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRAT 720
           DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQID E  +AT
Sbjct: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDAESNQAT 720

Query: 721 AKETELKTEEAISHINQETVVNEVEDTSEARKV 753
            KET++KTEEAIS IN+E VV EVEDT EAR+V
Sbjct: 721 TKETQVKTEEAISRINEENVVYEVEDTLEARRV 753

BLAST of Lsi09G000320 vs. NCBI nr
Match: XP_004145984.1 (uncharacterized protein LOC101216332 [Cucumis sativus] >KGN49875.1 hypothetical protein Csa_000201 [Cucumis sativus])

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 693/755 (91.79%), Postives = 713/755 (94.44%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADD+TIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDDTIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTHSHSTS P SANGFQNFKN APNARNVQTKVQ G TTP 
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSAPASANGFQNFKNAAPNARNVQTKVQVGPTTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 --QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFS 300
             QQQHQNSRQHPVS N YGTGRNAKN D GHSVGVNSVD+TNF WGPSSRT G GSNFS
Sbjct: 241 QQQQQHQNSRQHPVSSNTYGTGRNAKNPDTGHSVGVNSVDNTNFHWGPSSRTTGTGSNFS 300

Query: 301 SASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARG 360
           SASAQAAN VQQASEKVKRDRDETQ+S+EVERSHL SSKKKRTDGINN+GVHVANQ+ RG
Sbjct: 301 SASAQAANFVQQASEKVKRDRDETQASLEVERSHLTSSKKKRTDGINNFGVHVANQIVRG 360

Query: 361 DGSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKE 420
           DGSAG GLPE RKSY D+QKFHSFYGAFNR NSQRELSIFEIRNMLMDKARAEI KKLKE
Sbjct: 361 DGSAGDGLPESRKSYSDSQKFHSFYGAFNRNNSQRELSIFEIRNMLMDKARAEIRKKLKE 420

Query: 421 WSSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSEN 480
           W S+AEK+TLNKQSKKQKSV+NDGTHDIKINGKSSANGK  H RKPESDS+AGKNTGS  
Sbjct: 421 WRSMAEKATLNKQSKKQKSVLNDGTHDIKINGKSSANGKGWHGRKPESDSLAGKNTGSAK 480

Query: 481 DPISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRIS 540
           DPI+INVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVIS +PFRMRIS
Sbjct: 481 DPITINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISRKPFRMRIS 540

Query: 541 WLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQ 600
           WLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCW KGLRGVIRIFPQ
Sbjct: 541 WLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWAKGLRGVIRIFPQ 600

Query: 601 KGEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTH 660
           KGEVWALYRNWS DWNK+TSEEM+HKYDMVEVLDDFNEEQGVSV PLVK +GFRTVFRTH
Sbjct: 601 KGEVWALYRNWSVDWNKDTSEEMVHKYDMVEVLDDFNEEQGVSVAPLVKVIGFRTVFRTH 660

Query: 661 MDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRA 720
           MDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQID E  +A
Sbjct: 661 MDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDAESNQA 720

Query: 721 TAKETELKTEEAISHINQETVVNEVEDTSEARKVD 754
           T KET +KTEEAIS IN+E VVNEVEDT EARKVD
Sbjct: 721 TTKETRVKTEEAISCINEENVVNEVEDTLEARKVD 755

BLAST of Lsi09G000320 vs. NCBI nr
Match: XP_008437584.1 (PREDICTED: uncharacterized protein LOC103482955 [Cucumis melo])

HSP 1 Score: 1386.3 bits (3587), Expect = 0.0e+00
Identity = 694/752 (92.29%), Postives = 712/752 (94.68%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTHSHSTS PPSANGFQNFKN APNARNVQTKVQ G TTP 
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSAPPSANGFQNFKNAAPNARNVQTKVQVGPTTPF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS
Sbjct: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240

Query: 241 -QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSS 300
            QQQHQNSRQH VSGN YGTGRNAKN D GHSVGVNSVD+TNF W PSSR  G GSNFSS
Sbjct: 241 QQQQHQNSRQHSVSGNTYGTGRNAKNPDTGHSVGVNSVDNTNFHWSPSSRPTGTGSNFSS 300

Query: 301 ASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGD 360
           ASAQAANLVQQASEKVKRDRDETQ S+EVERSHL SSKKKRTDGINNYGVHVANQMARGD
Sbjct: 301 ASAQAANLVQQASEKVKRDRDETQPSLEVERSHLTSSKKKRTDGINNYGVHVANQMARGD 360

Query: 361 GSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEW 420
           GSAG GLPE RKSY DTQKFHSFYGAFN  NSQRELSIFEIRNMLMDKARAEI KKLKEW
Sbjct: 361 GSAGDGLPETRKSYSDTQKFHSFYGAFNNNNSQRELSIFEIRNMLMDKARAEIRKKLKEW 420

Query: 421 SSLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSEND 480
            S+AEK+TLNKQSKKQKSV+NDGT DIKINGKSSANGK  H RKPESDS+AGKNTGS ND
Sbjct: 421 RSMAEKATLNKQSKKQKSVLNDGTPDIKINGKSSANGKGWHGRKPESDSLAGKNTGSAND 480

Query: 481 PISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISW 540
           PI+INVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISL+PFRMRISW
Sbjct: 481 PITINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLKPFRMRISW 540

Query: 541 LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600
           LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK
Sbjct: 541 LNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQK 600

Query: 601 GEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHM 660
           GEVWALYRNWS DWNK+TSEEM+HKYDMVEVLDDFNEEQGVSV PLVK +GFRTVFRT+M
Sbjct: 601 GEVWALYRNWSVDWNKDTSEEMVHKYDMVEVLDDFNEEQGVSVAPLVKVIGFRTVFRTNM 660

Query: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRAT 720
           DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQID E  +AT
Sbjct: 661 DPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDAESNQAT 720

Query: 721 AKETELKTEEAISHINQETVVNEVEDTSEARK 752
            KET++KTEEAIS IN+E VV EVEDT EAR+
Sbjct: 721 TKETQVKTEEAISRINEENVVYEVEDTLEARR 752

BLAST of Lsi09G000320 vs. NCBI nr
Match: XP_022973026.1 (uncharacterized protein LOC111471541 [Cucurbita maxima] >XP_022973027.1 uncharacterized protein LOC111471541 [Cucurbita maxima] >XP_022973028.1 uncharacterized protein LOC111471541 [Cucurbita maxima])

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 695/773 (89.91%), Postives = 728/773 (94.18%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEAARAKEIAERKF+ERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK
Sbjct: 1   MECNKDEAARAKEIAERKFTERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           INGE DWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK
Sbjct: 61  INGEMDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
           AKRLAYNQKRDLKGGRQKTPTH+HSTSVPPSANGFQ+FKNVA NARNVQ+KV  GATTPL
Sbjct: 121 AKRLAYNQKRDLKGGRQKTPTHTHSTSVPPSANGFQSFKNVASNARNVQSKVHVGATTPL 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
           QPS RKP+TFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVE+APPPNV KSPSWSS
Sbjct: 181 QPSQRKPDTFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVERAPPPNVLKSPSWSS 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPSSRTAGVGSNFSSA 300
           QQQHQNSRQ+  +G+ YGTGRN+K  D GHSVG+NSVD+TNFQWGPSSRTAGVGS FSSA
Sbjct: 241 QQQHQNSRQY-TTGDAYGTGRNSKKPDSGHSVGINSVDNTNFQWGPSSRTAGVGSTFSSA 300

Query: 301 SAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMARGDG 360
           SA+AANL QQASEKVKRDRDETQ S EV+RS L SSKKKRTDGIN++GVH ANQMA GDG
Sbjct: 301 SARAANLGQQASEKVKRDRDETQPSTEVDRSQL-SSKKKRTDGINSFGVHAANQMASGDG 360

Query: 361 SAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDKARAEICKKLKEWS 420
           SAGVGL ELRKSYPDTQKFHSFY AFNR +SQRELSIFEIRNMLMDK+RAEI KKLKEWS
Sbjct: 361 SAGVGLSELRKSYPDTQKFHSFYYAFNRIDSQRELSIFEIRNMLMDKSRAEIHKKLKEWS 420

Query: 421 SLAEKSTLNKQSKKQKSVVNDGTHDIKINGKSSANGKRGHERKPESDSIAGKNTGSENDP 480
           SLAEKSTLNKQSKKQK+VVNDGTHDIKINGK SANGK  +E++PESDS+A KNTGSENDP
Sbjct: 421 SLAEKSTLNKQSKKQKNVVNDGTHDIKINGKPSANGKGWNEKEPESDSLASKNTGSENDP 480

Query: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVISLRPFRMRISWL 540
           ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKV+SLRPFRMR SWL
Sbjct: 481 ISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHKVMSLRPFRMRFSWL 540

Query: 541 NSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKG 600
           NSRSN EIGPMDWIGSGFTKTCGDFRIG+HEVTRSLNSFSHKVCWVKGLRGVI+IFPQKG
Sbjct: 541 NSRSNMEIGPMDWIGSGFTKTCGDFRIGKHEVTRSLNSFSHKVCWVKGLRGVIQIFPQKG 600

Query: 601 EVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHMD 660
           EVWALYRNWSADWNK+TSEEMIHKYDMVEVLDDFNE++GVSV PLVK  GFRTVF  +M 
Sbjct: 601 EVWALYRNWSADWNKDTSEEMIHKYDMVEVLDDFNEKEGVSVAPLVKVGGFRTVFCRNMH 660

Query: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPLELLQIDTELKRATA 720
           PKEVRKIPKEEMFRFSHQVPNYLLTG+EAQNAPKGCRELDPAATPLELLQIDTE  + T 
Sbjct: 661 PKEVRKIPKEEMFRFSHQVPNYLLTGDEAQNAPKGCRELDPAATPLELLQIDTESVQVTT 720

Query: 721 KETELKTEEAISHINQETVVNEVEDTSEARKVDVVTQEDEQTKMSGDVAFQGS 774
           KETE+KT   ISHINQETVVNEVEDT EARKVDVVTQEDEQTKMSGDV FQGS
Sbjct: 721 KETEVKTVGEISHINQETVVNEVEDTLEARKVDVVTQEDEQTKMSGDVTFQGS 771

BLAST of Lsi09G000320 vs. TAIR 10
Match: AT5G53150.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-162
Identity = 352/801 (43.95%), Postives = 471/801 (58.80%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           MECNKDEA RA +IAERK +E++Y+ AKKF  KAQNL+P LDGL Q+   + VYIS E  
Sbjct: 1   MECNKDEAKRAMDIAERKMTEKDYTGAKKFANKAQNLFPELDGLKQLFVAINVYISGEKT 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
             GE DWYG+LGV+  A DE ++KQYRKL L+LHPDKNK  GAEGAF LV+EAW+LLSDK
Sbjct: 61  FAGEADWYGVLGVDPFASDEALKKQYRKLVLMLHPDKNKCKGAEGAFNLVAEAWALLSDK 120

Query: 121 AKRLAYNQKR--DLKGGRQKTPTHSHS-TSVPPSANGFQNFKNVAPNARNVQTKVQAGAT 180
            KR+ YN KR  D+K  +Q+ PT      S  P++NG            NV+  V + A 
Sbjct: 121 DKRILYNVKRGKDVKAAQQRFPTTQREIPSHQPTSNGIP----------NVREHVVSSAR 180

Query: 181 TPLQPSLRKP---------------------ETFWTLCNRCKTHYEYLRVYLNHTLLCPN 240
              +P+ RKP                      TFWT+CN+C T YEY RVYLN TLLCP+
Sbjct: 181 ARYKPATRKPAARMDRSRTGSPAFVYPTQESSTFWTMCNKCDTQYEYQRVYLNQTLLCPH 240

Query: 241 CHEAFLAVEKAPPPNVFKSP-SWSSQQQHQNSRQHPVSGNVYGTG-RNAKNSDIGHSVGV 300
           CH  F+A EK PP N+ K P + SS Q H++S+    + N  G+  R    + + H    
Sbjct: 241 CHHGFVAEEKTPPTNIPKPPVNISSNQHHRSSKNQASNKNSNGSSYRREPATSVNH---- 300

Query: 301 NSVDSTNFQWGPSSRTAGVGSNFSSASAQAANLVQQASEKVKR---DRDETQSSIEVERS 360
                 NFQW  SSR    GS   +A+ + AN+VQQ  +K+KR   +  E +++     S
Sbjct: 301 ------NFQW-DSSRMG--GSYSRNATNETANVVQQGQDKLKRVFWETQEREAARGFTNS 360

Query: 361 HLPSSKKKRTDGINNYGVHVANQMARGDGSAGVGLPELRKSYPDTQKFHSFYGAFNRTNS 420
            L + K+++TD  +  G                         P     H +  A  R+  
Sbjct: 361 DLGNFKRQKTDDSHMRG-------------------------PSAGSRHPYVQALLRS-- 420

Query: 421 QRELSIFEIRNMLMDKARAEICKKLKEWSSLAEKSTLNKQSKKQ--KSVVNDGTHDIKIN 480
                  +I+  LMD+ ++EI K+L    +  E      + +K   K++ +  +   +  
Sbjct: 421 -------DIKKALMDRGQSEIFKRLPMMIAKMEGKVNPTEGEKNSTKAMSSKASEVERSK 480

Query: 481 GKSSANG-KRGHERKP-ESDSIAGKNTGSENDPISINVPDPDFHNFDLDRAESSFGDDQV 540
             S+AN  +R  E  P ESD +             I VPD DFHNFDLDR+ES+F DDQ+
Sbjct: 481 MSSTANEVERSVEVIPHESDEVK-----------EIVVPDSDFHNFDLDRSESAFKDDQI 540

Query: 541 WACYDDDDGMPRFYARIHKVISLRPFRMRISWLNSRSNTEIGPMDWIGSGFTKTCGDFRI 600
           WA YDD DGMPRFYARI KVIS+ PF+++ISWLNS++ +E GP+DW+G+GF K+CGDFR 
Sbjct: 541 WAAYDDADGMPRFYARIQKVISVNPFKLKISWLNSKTTSEFGPIDWMGAGFAKSCGDFRC 600

Query: 601 GRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKGEVWALYRNWSADWNKNTSEEMIHKYDM 660
           GR+E T +LN+FSH V + KG RG++ I P+KG+VWALYRNWS +W+KNT +E+ HKY+M
Sbjct: 601 GRYESTDTLNAFSHSVDFTKGARGLLHILPKKGQVWALYRNWSPEWDKNTPDEVKHKYEM 660

Query: 661 VEVLDDFNE-EQGVSVTPLVKEVGFRTVFRTHMDPKEVRKIPKEEMFRFSHQVPNYLLTG 720
           VEVLDD+ E +Q ++V  L+K  GFR VFR   +   VRKI KEEM RFSHQVP+Y+LTG
Sbjct: 661 VEVLDDYTEDDQSLTVALLLKAEGFRVVFRRCTEKLGVRKIAKEEMLRFSHQVPHYILTG 720

Query: 721 EEAQNAPKGCRELDPAATPLELLQIDTELKRATAKETELKTEEAISHINQETVVNEVEDT 768
           +EA NAP+G  ELDPAATP      + E    +    E +  EA+   N+E+   +  + 
Sbjct: 721 KEADNAPEGFLELDPAATPCAFSSENAEADEKSEAVKENEQGEAVKE-NEESEALKENEE 726

BLAST of Lsi09G000320 vs. TAIR 10
Match: AT2G25560.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 453.0 bits (1164), Expect = 4.9e-127
Identity = 283/724 (39.09%), Postives = 402/724 (55.52%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           ME NK+EA RA+EIA+RKF   +++ A+KF LKAQ LYP LDG++QM+ T +V++SA+N 
Sbjct: 1   MEFNKEEATRAREIAKRKFLANDFAGARKFALKAQFLYPELDGIAQMVATFDVHLSAQNI 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           I G+ D YG+LG+N  ADDE +RK+YRKLA++LHPD+NKS+GAE AFK +S+AW + SDK
Sbjct: 61  IYGDVDHYGVLGLNPEADDEIVRKRYRKLAVMLHPDRNKSVGAEEAFKFLSQAWGVFSDK 120

Query: 121 AKRLAYNQKRDL---KGGRQKTPTHSHSTSVPPSANGFQ-------NFKNVAPNARNVQT 180
           AKR  Y+ KR++   KGG         ++S  P+ NGFQ       N   V  + R ++ 
Sbjct: 121 AKRADYDLKRNVGLYKGG--------GASSSRPATNGFQKVTKASGNTTKVKSSKRGIKR 180

Query: 181 KVQAGATTPLQPSLRKPE---TFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKA 240
              A A      S +K     TFWT+C  C+T YEY  VYLN  LLCPNC + F+AVE  
Sbjct: 181 ASDASAAATTSTSAQKTTADGTFWTVCRTCRTQYEYHSVYLNQNLLCPNCRKPFIAVETD 240

Query: 241 PPPNVFKSPSWSSQQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQWGPS 300
           PP          S    +   +H      + T    KN     + GV   +  +F+WG  
Sbjct: 241 PP---------GSGSIRKTFHEHQFDSLRHTTDGRKKNVPGRDNNGVYG-EYDSFEWGVF 300

Query: 301 SRTAGVGSNFSSASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNY 360
           + T          SA A     +  E V+R+  +  +      S +P  ++K        
Sbjct: 301 TGT--------KTSAHATPTGSRKDEVVRREYTKRTAG---PSSTIPPKRRK-------- 360

Query: 361 GVHVANQMARGDGSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRNMLMDK 420
              V      G   A    P                    ++   +E+S  E++N+L  K
Sbjct: 361 ---VMENAVAGGNIASCLAP--------------------KSTGVKEVSEDELKNLLKKK 420

Query: 421 ARAEICKKLKEWSSLAEKSTLNKQSKKQKSVVNDGTHDIK-INGKSSANGKRGHERKPES 480
           A++ I + L E  ++  ++  N +  +        T D+   N  SS N     +   ES
Sbjct: 421 AKSVISRNLPELCTIVAETETNGRGME--------TEDLNGFNAGSSVN-----KNAIES 480

Query: 481 DSIAGKNTGSENDPISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPRFYARIHK 540
             +      +    ++++V  PDF +FD DR E S  D+Q+WA YD  +G+PR YA IH 
Sbjct: 481 CCMNTDKELNSLGALTLDVTAPDFCDFDKDRTEKSVKDNQIWAFYDSHEGLPRSYALIHN 540

Query: 541 VISLRPFRMRISWLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSFSHKVCWV 600
           VIS+ PF++R+SWL   +N E    +W+G G  K+CG FR+ +  + RS  SFSHKV  V
Sbjct: 541 VISVDPFKVRMSWLTPVTNGEPSSTNWLGFGIPKSCGGFRVRKTLIYRSPYSFSHKVNLV 600

Query: 601 KGLRGVIRIFPQKGEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQGVSVTPLV 660
           KG  G   I+P+ G+VWALYR WS DWN  T  E + +YD+VEV++ + EE GV V PLV
Sbjct: 601 KGNHGEFLIYPRTGDVWALYRKWSPDWNYLTGVETV-EYDIVEVVEGYTEEYGVVVVPLV 650

Query: 661 KEVGFRTVFRTHMDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRELDPAATPL 711
           K  GF+ VF  H+D KE ++  ++E+ RFSH++P+YLLTG+EA  AP+GCR+LDPAATP 
Sbjct: 661 KVAGFKAVFHHHLDSKETKRFLRDEISRFSHKIPSYLLTGQEAPGAPRGCRQLDPAATPS 650

BLAST of Lsi09G000320 vs. TAIR 10
Match: AT2G05250.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 386.3 bits (991), Expect = 5.6e-107
Identity = 252/733 (34.38%), Postives = 385/733 (52.52%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           ME  ++EA R K+IAER+F+E+++++A+ + LKA++L+P L+GLSQM+ T EVY++++ +
Sbjct: 1   MEAYREEALRVKQIAERRFAEKDFTSARSYALKAKSLFPDLEGLSQMVATFEVYLASQTR 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
             G+ D+Y +LG+   A    ++KQY+K+A++LHPDKNK +GA+GAF L+SEAWS LS++
Sbjct: 61  SGGQIDYYAVLGLKPSAGKREVKKQYKKMAVLLHPDKNKCIGADGAFHLISEAWSFLSNE 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
             +  +  KR  K     T    HST   P                   T          
Sbjct: 121 FNKSTFYYKR--KKHIDSTEVQKHSTEYMPGTG----------------TGTGTAVFDRF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
            PS  + +TFWT+C  CK  YEYLR Y+N  L C NC  AF+AVE  P P    +P   +
Sbjct: 181 PPSSERLDTFWTVCTSCKVQYEYLRKYVNKRLSCKNCRGAFIAVETGPAP--VSAPFHYT 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSV------DSTNFQWGPSSRTAGVG 300
              H      P S      G  A   D    +  NS             +  S+  +   
Sbjct: 241 PPSHAPPSHAPPSHAPPSNGYGAHGYDAMSRMPTNSTYFLGHYPGQGHGYDYSTNGSYEW 300

Query: 301 SNFSSASAQAANLVQQASEKV------KRDRDETQSSI-EVERSHLPSSKKKRTDGINNY 360
           S++S  +    NL  +    V      K         I +V+     +  KK T G    
Sbjct: 301 SSYSGTTTSPGNLDLKRVSSVSNGYPYKHSNSVVSGGINKVKDGSNGTCSKKSTPG---- 360

Query: 361 GVHVANQMARGDGSAG--VGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRN--M 420
           G+   NQ++    ++   VG P  +         + F       N  R +S+ +  N   
Sbjct: 361 GLIYPNQISMSAHASANKVGRPGKKSKVFMEAAANGFV-----ENPLRSVSVSKTANTDA 420

Query: 421 LMDK-ARAEICKKLKEWSSLAEKSTLNKQSKKQKSVVNDGTHDIK--INGKSSANGKRGH 480
            MD+  +  I    + WS+ +   T     +K ++ +      ++  +   ++A      
Sbjct: 421 KMDQDYKLHIQSSTRRWSAASVLDTRKPLIQKARTDIKQRLEMMRLALEAAAAAEDATPL 480

Query: 481 ERKPESDSIAGKNTGSE-NDPISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPR 540
           + K       G  TG + N PI+  VPD DFH+FD +R+E SF   Q+WA YD+DDGMPR
Sbjct: 481 DEKTVLSCKLGDVTGRKTNGPIT--VPDSDFHDFDKNRSEESFEPRQIWAIYDEDDGMPR 540

Query: 541 FYARIHKVISLRPFRMRISWLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSF 600
            Y  + +V+S++PF++ I++L+S+++ E G M W+  GFTK+CG FRI   ++   +N F
Sbjct: 541 LYCVVREVLSVQPFKIDIAYLSSKTDIEFGSMKWVQYGFTKSCGHFRIRNSDIVDHVNIF 600

Query: 601 SHKVCWVK-GLRGVIRIFPQKGEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQ 660
           SH +   K G  G +RIFP  GE+WA+Y+NWS +W+ +T +E+ H+Y+MVE+LD++ E+ 
Sbjct: 601 SHLLKGKKTGRGGCVRIFPTAGEIWAVYKNWSLNWDGSTPDEVRHQYEMVEILDEYTEQY 660

Query: 661 GVSVTPLVKEVGFRTVFRTHMDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRE 712
           GV VTPLVK  G++TV+         + IP+ EM RFSHQVP++ L  +     P+ C +
Sbjct: 661 GVCVTPLVKLEGYKTVYHRSTREDSKKWIPRCEMLRFSHQVPSWFLK-DATSGFPENCWD 701

BLAST of Lsi09G000320 vs. TAIR 10
Match: AT2G05230.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 386.3 bits (991), Expect = 5.6e-107
Identity = 252/733 (34.38%), Postives = 385/733 (52.52%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           ME  ++EA R K+IAER+F+E+++++A+ + LKA++L+P L+GLSQM+ T EVY++++ +
Sbjct: 1   MEAYREEALRVKQIAERRFAEKDFTSARSYALKAKSLFPDLEGLSQMVATFEVYLASQTR 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
             G+ D+Y +LG+   A    ++KQY+K+A++LHPDKNK +GA+GAF L+SEAWS LS++
Sbjct: 61  SGGQIDYYAVLGLKPSAGKREVKKQYKKMAVLLHPDKNKCIGADGAFHLISEAWSFLSNE 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
             +  +  KR  K     T    HST   P                   T          
Sbjct: 121 FNKSTFYYKR--KKHIDSTEVQKHSTEYMPGTG----------------TGTGTAVFDRF 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
            PS  + +TFWT+C  CK  YEYLR Y+N  L C NC  AF+AVE  P P    +P   +
Sbjct: 181 PPSSERLDTFWTVCTSCKVQYEYLRKYVNKRLSCKNCRGAFIAVETGPAP--VSAPFHYT 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSV------DSTNFQWGPSSRTAGVG 300
              H      P S      G  A   D    +  NS             +  S+  +   
Sbjct: 241 PPSHAPPSHAPPSHAPPSNGYGAHGYDAMSRMPTNSTYFLGHYPGQGHGYDYSTNGSYEW 300

Query: 301 SNFSSASAQAANLVQQASEKV------KRDRDETQSSI-EVERSHLPSSKKKRTDGINNY 360
           S++S  +    NL  +    V      K         I +V+     +  KK T G    
Sbjct: 301 SSYSGTTTSPGNLDLKRVSSVSNGYPYKHSNSVVSGGINKVKDGSNGTCSKKSTPG---- 360

Query: 361 GVHVANQMARGDGSAG--VGLPELRKSYPDTQKFHSFYGAFNRTNSQRELSIFEIRN--M 420
           G+   NQ++    ++   VG P  +         + F       N  R +S+ +  N   
Sbjct: 361 GLIYPNQISMSAHASANKVGRPGKKSKVFMEAAANGFV-----ENPLRSVSVSKTANTDA 420

Query: 421 LMDK-ARAEICKKLKEWSSLAEKSTLNKQSKKQKSVVNDGTHDIK--INGKSSANGKRGH 480
            MD+  +  I    + WS+ +   T     +K ++ +      ++  +   ++A      
Sbjct: 421 KMDQDYKLHIQSSTRRWSAASVLDTRKPLIQKARTDIKQRLEMMRLALEAAAAAEDATPL 480

Query: 481 ERKPESDSIAGKNTGSE-NDPISINVPDPDFHNFDLDRAESSFGDDQVWACYDDDDGMPR 540
           + K       G  TG + N PI+  VPD DFH+FD +R+E SF   Q+WA YD+DDGMPR
Sbjct: 481 DEKTVLSCKLGDVTGRKTNGPIT--VPDSDFHDFDKNRSEESFEPRQIWAIYDEDDGMPR 540

Query: 541 FYARIHKVISLRPFRMRISWLNSRSNTEIGPMDWIGSGFTKTCGDFRIGRHEVTRSLNSF 600
            Y  + +V+S++PF++ I++L+S+++ E G M W+  GFTK+CG FRI   ++   +N F
Sbjct: 541 LYCVVREVLSVQPFKIDIAYLSSKTDIEFGSMKWVQYGFTKSCGHFRIRNSDIVDHVNIF 600

Query: 601 SHKVCWVK-GLRGVIRIFPQKGEVWALYRNWSADWNKNTSEEMIHKYDMVEVLDDFNEEQ 660
           SH +   K G  G +RIFP  GE+WA+Y+NWS +W+ +T +E+ H+Y+MVE+LD++ E+ 
Sbjct: 601 SHLLKGKKTGRGGCVRIFPTAGEIWAVYKNWSLNWDGSTPDEVRHQYEMVEILDEYTEQY 660

Query: 661 GVSVTPLVKEVGFRTVFRTHMDPKEVRKIPKEEMFRFSHQVPNYLLTGEEAQNAPKGCRE 712
           GV VTPLVK  G++TV+         + IP+ EM RFSHQVP++ L  +     P+ C +
Sbjct: 661 GVCVTPLVKLEGYKTVYHRSTREDSKKWIPRCEMLRFSHQVPSWFLK-DATSGFPENCWD 701

BLAST of Lsi09G000320 vs. TAIR 10
Match: AT5G27240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 291.6 bits (745), Expect = 1.9e-78
Identity = 231/747 (30.92%), Postives = 350/747 (46.85%), Query Frame = 0

Query: 1   MECNKDEAARAKEIAERKFSERNYSAAKKFVLKAQNLYPGLDGLSQMMTTLEVYISAENK 60
           M+ NK+EA RAK +AE K  E ++  A+K +LKAQ+L+ GL+ L QM+   +V+ SAE K
Sbjct: 1   MDWNKEEACRAKTLAEDKMKEGDFVGAQKLLLKAQSLFSGLESLPQMLAVCDVHNSAEKK 60

Query: 61  INGETDWYGILGVNHLADDETIRKQYRKLALVLHPDKNKSLGAEGAFKLVSEAWSLLSDK 120
           IN   +WYGIL V H ADD TI+KQ RKLAL+LHPDKN+  GAE AFKLV +A   L+DK
Sbjct: 61  INCLENWYGILQVMHFADDATIKKQVRKLALLLHPDKNQFPGAEAAFKLVWDASRFLADK 120

Query: 121 AKRLAYNQKRDLKGGRQKTPTHSHSTSVPPSANGFQNFKNVAPNARNVQTKVQAGATTPL 180
            KR  Y+ +R +                         +  +A N  N  + +Q  AT   
Sbjct: 121 DKRSQYDIRRRI-------------------------YLRLATNQLNANSGLQCAATN-- 180

Query: 181 QPSLRKPETFWTLCNRCKTHYEYLRVYLNHTLLCPNCHEAFLAVEKAPPPNVFKSPSWSS 240
                  +TFWT C  C   Y+YLR Y+N  L C  C  +++A +     N   S S + 
Sbjct: 181 ----SATDTFWTCCEHCGYRYKYLRKYVNILLNCNICQRSYMAYDTG--FNEAPSKSNTG 240

Query: 241 QQQHQNSRQHPVSGNVYGTGRNAKNSDIGHSVGVNSVDSTNFQ----WGPSSRTAGVGSN 300
           Q++ QN      S N  G    A+   +   V      +  F      G S +T    S 
Sbjct: 241 QKEVQNQGPCNTSLNTNGESIGAQPGSVAAEVDKKGTFNKKFNKRNGGGESKKTEVKNSK 300

Query: 301 FSSASAQAANLVQQASEKVKRDRDETQSSIEVERSHLPSSKKKRTDGINNYGVHVANQMA 360
             +   +         EK+K      +   EV    + +SK    + ++      +    
Sbjct: 301 IETEFVR-----NDDEEKMKSAAKLHKPHPEVTEPEIGASKSVPDESVSRSDEAPSTSKD 360

Query: 361 RGDGSAGVGLPELRKSYPDTQKFHSFYGAFNRTNSQR--ELSIFEIRNMLMDKARAEICK 420
           +     GV    +     D  K  ++    N+  S R  + S +     + D +     K
Sbjct: 361 KNKMKKGVE-ESINVDGSDFIKDKAYSKDNNKRKSPRRSQQSSYAEEEKISDNSLGPPKK 420

Query: 421 KLKEWSSLAEKSTLNKQ-----------------------SKKQKSVVNDGTHDIKINGK 480
           +L+    L  + T  K                         +K K  V+ G  +I     
Sbjct: 421 RLRSNVGLKSEQTTRKGLVGVGSSKRFDSGGGSSAPSCAFKRKAKKFVDSGYQEI----- 480

Query: 481 SSANGKRGHERKPESDSIA-----GKNTGSENDPISINVPDPDFHNFDLDRAESSFGDDQ 540
           SS   K    RK   + +        N     + I+ ++PDP+F NF+L    S FG +Q
Sbjct: 481 SSVKDKEREVRKASGEGVVMEAKIDNNHNPNENLITEDLPDPEFSNFEL--TTSCFGVNQ 540

Query: 541 VWACYDDDDGMPRFYARIHKVISLRPFRMRISWLNSRSNTEIGPMDWIGSGFTKTCGDFR 600
           VW+ YD  DGMPR YARI KV+ +  F++ I+W++   + +        +     CG F+
Sbjct: 541 VWSMYDPIDGMPRLYARIDKVL-VPEFKLWITWIDPLQDNK-------DNSIPIACGIFQ 600

Query: 601 IGRHEVTRSLNSFSHKVCWVKGLRGVIRIFPQKGEVWALYRNWSADWNKNT-SEEMIHKY 660
            G  E       FS ++  +     V+ I+P+KGE+WA++R W   W+ ++ + +  ++Y
Sbjct: 601 GGGSEEENDHLKFSCQMFHLTRNNSVV-IYPRKGEIWAIFRGWDISWSASSENHKHPYEY 660

Query: 661 DMVEVLDDFNEEQGVSVTPLVKEVGFRTVFRTHMDPKEVR-KIPKEEMFRFSHQVPNYLL 712
           D VEVL +FN+E G+ V  L K  GF ++FR       ++ +IP  +M RFSH+VP++ +
Sbjct: 661 DFVEVLSNFNDENGLGVGFLGKVEGFVSLFRQDAQDGVLQLQIPPSQMLRFSHKVPSFKM 692

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q149L66.0e-1331.55DnaJ homolog subfamily B member 14 OS=Mus musculus OX=10090 GN=Dnajb14 PE=2 SV=1[more]
Q9QYI47.8e-1332.63DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2[more]
Q58DR21.3e-1233.51DnaJ homolog subfamily B member 12 OS=Bos taurus OX=9913 GN=DNAJB12 PE=2 SV=1[more]
Q9NXW21.1e-1130.32DnaJ homolog subfamily B member 12 OS=Homo sapiens OX=9606 GN=DNAJB12 PE=1 SV=5[more]
Q9FH281.5e-1133.54Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3C4030.0e+0092.16Curved DNA-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A0A0KJH00.0e+0091.79J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139530 PE=4 SV=1[more]
A0A1S3AV000.0e+0092.29uncharacterized protein LOC103482955 OS=Cucumis melo OX=3656 GN=LOC103482955 PE=... [more]
A0A6J1I7J90.0e+0089.91uncharacterized protein LOC111471541 OS=Cucurbita maxima OX=3661 GN=LOC111471541... [more]
A0A6J1E3630.0e+0089.39uncharacterized protein LOC111430376 OS=Cucurbita moschata OX=3662 GN=LOC1114303... [more]
Match NameE-valueIdentityDescription
XP_038874482.10.0e+0094.05uncharacterized protein LOC120067126 [Benincasa hispida][more]
KAA0042558.10.0e+0092.16Curved DNA-binding protein [Cucumis melo var. makuwa] >TYK05962.1 Curved DNA-bin... [more]
XP_004145984.10.0e+0091.79uncharacterized protein LOC101216332 [Cucumis sativus] >KGN49875.1 hypothetical ... [more]
XP_008437584.10.0e+0092.29PREDICTED: uncharacterized protein LOC103482955 [Cucumis melo][more]
XP_022973026.10.0e+0089.91uncharacterized protein LOC111471541 [Cucurbita maxima] >XP_022973027.1 uncharac... [more]
Match NameE-valueIdentityDescription
AT5G53150.11.6e-16243.95DNAJ heat shock N-terminal domain-containing protein [more]
AT2G25560.14.9e-12739.09DNAJ heat shock N-terminal domain-containing protein [more]
AT2G05250.15.6e-10734.38DNAJ heat shock N-terminal domain-containing protein [more]
AT2G05230.15.6e-10734.38DNAJ heat shock N-terminal domain-containing protein [more]
AT5G27240.11.9e-7830.92DNAJ heat shock N-terminal domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 102..122
score: 43.98
coord: 68..86
score: 41.57
coord: 86..101
score: 55.23
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 65..122
e-value: 7.3E-24
score: 95.3
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 66..127
e-value: 2.4E-20
score: 72.4
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 66..130
score: 17.192774
IPR001623DnaJ domainCDDcd06257DnaJcoord: 66..119
e-value: 5.36962E-21
score: 84.9045
IPR024593Domain of unknown function DUF3444PFAMPF11926DUF3444coord: 482..688
e-value: 3.5E-75
score: 252.3
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 63..175
e-value: 7.3E-23
score: 83.2
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 58..126
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 139..153
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..153
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 754..773
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..272
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 454..468
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..339
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 233..272
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 304..343
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 424..483
NoneNo IPR availablePANTHERPTHR44137BNAC03G44070D PROTEINcoord: 1..727
NoneNo IPR availablePANTHERPTHR44137:SF32HEAT SHOCK PROTEIN-LIKEcoord: 1..727
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 107..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi09G000320.1Lsi09G000320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003677 DNA binding