Lsi08G016710 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G016710
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionBOI-related E3 ubiquitin-protein ligase 1-like
Locationchr08: 24463244 .. 24466479 (+)
RNA-Seq ExpressionLsi08G016710
SyntenyLsi08G016710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTTTTAAAAAAAAAGGAACGGAAATCCGTTTGCGTAACCACTACCACCGATTCATTCATTTCATTCTAATAAATTTTCCACTCCCACCGCACCACCTCCCTACAATTTCTCTTCCTCTCTCTCTCTCTCTCTCATATAATTCTTCATCTTCATCATCATCTTCTTCTTCTTCTCGTCGGTTACCTTACCGCTCCAGAAGCCTCCATCCATCTCTCCCCTTTCCTCTATATATTTATGTATGTATATTTCTAGATCCTATTCCTCCTCCTTCATCATTGTCCCCACCTTTGGATTCTACATACTCAATCTTCAAGGTATTCCATCTTTTATTTCTATTTTCTTTTCGTTATTACTATGTGTATATATGTATTGCTGCGAATTATGTGGATAAATCTGTGTATAATGTTGTATGGCAGGATCTGGAGATTATATTTCTTCTCATCTGACACTGCCCAAATGACTTCTTCTTGAGGTAACCCTTTCCTTCCTGTTTCAGACCCTAGTTTCATCTTTGCATCGTTCCTAAAGTCGTGCCTAATAAATCCGTAGATGATTATCTCAAGTAAACTTGTTCCTTGTCAAAATTGTTAGATTGCCTGTTCCTTCATTATGCGATTCCAGTCAAACCTGCTCATCAATCTCCCTAACTTTCCTGATTTAGAGATCCCCACTTATGATACGCTAAACCTTTTCCCATTCCCCTACCGCGTGCCCATCCCTGCTGATGTTTTTCTGATTATCTGGATATGGTTTTGTGATTGAACTACTGCTATTTCCTTCTGATCTGATCTCCCTCTAGTGTTATTTCTGAATTCGTGTTTTCCTTGAATTTGATGTTTATATTTGACTTTCTGCTATCAAGGTTGATAAGGAAAACATGATGATGGGCAGTTTCTCGTATTTTCACCTATTTGGATGGATTGTTAGTCTAAGTAAATGTTACGGATAACTGGATTTTGTAAGATTACTGTGATTGCCCATTACTGGAGTTTCAAATACTGAATTAAGTCCTGGAGTATCATACCCATGTTTCGTAATAGGATTTGTTGAATATGAGCCCTCCGTTGTTTTATTGTATTTGGGAAGAATTTAACATAAAAGCTTTTGTGGCAATGCGAAGTCTGTTCAAGAACTTGTTTAATCCGAGGTTTTTGGTATTTCTGCAAACTTGCTGACCGAATCCTCTTATTTTGTCGGATGTTTTTCCAATCAATCCCCTTCGTCCCCTCTTGGTTAAATAATTTGAAGTTGAGGTGGTTCAAGGTCTAAGCCATGTTTCTTGTTTGTGTGGCGATGCTAAAGTGAGCATAGGTCAATGGTAATTAGCATATGAACTTTTTCGTTTGAGGTTGGAGCTTTATATCTCCATACTCCACTTGTACTAAAAAGATGTTTATGTAGTGTAATAATTCGTAATATTGTCATTTTCCTTCCTCGCAGTTTTATTCCAGCACTGAAATAGATTATTAAGACCGTTGTCTCCTTTTGCAGGCAGTGAACCCTCAGTACTGTTGGTATTTTAATTTTCATATTATCTGAAGTTCATCAGTTCAGTTCTGGGGAAAATGTTGGGTGGAAACAATGGCAATGGCTTGCTTCCCGTTTTCCTGGATGAAAATAGAACTCAGTATCAAACTAATGCTTCTGGTCAGCTGCAGTTATTTGGGAATTGTGAGTTGTTATTCTGTCTCTAAGAAATCTGATTTCAACTTACTGCATTCCATACTTTTTTATTTTTTTATTATGTCTAGCCAGAGTAAATGACTTGAATAACAATACAAGTCTGATAAGTATTTGGAAGATTAATCAGAACTTGTTAATATTCTTTAGTTGTCTCGTCTCTCATAGAATTCGCAATTTATGTTGTAGTGCCTGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGTTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAATTAGAAGATTTCTCAAGGCAGCAGAAGCTTCAGATTTCCCTAAATTATGCCTGTCGAGATGATGCTGATCGTTCAGCAAGCATTAGAAACCCAAATGCTGTATCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACCACCGCAAGTGGAAGTATGACAGCTGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGTTCTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAACTTCAGGTATTTCTAATCTCCTCAGTGTTTCTAGGACTTTCTGCTCTTCTGGATTTTTTACGAGACTGCATGCTGCATAGGGAGGGATAAAACTAAAATAATTTATGATGTTTTTTCCTGGAAGATGTTTGTGGTCGAAGCCTGATTAGTTCATGAAGACCTTTGTATTACTCTTTATAGCAATTACTTCACACGATGCCTGTAAATGGGGTGCTAATGTTAAGTTTCCTCAATGTGGTTGATTCAATTGCATCTGATGTGTTAGATTTTCCATCTGCCTTGTGAATAGGAAGAACATTTGACAAAGGGTATTAGGGACATGAAGCAAAGACATATGGCTTCCTTTCTTAGTGCGATTGAGAAAGGTATTGGAAAGAAACTACATGAAAAGGATGTTGAAATTGAGAGCATGAACCGTAAAAACAGAGAACTAGTGGAGAGAATAAAGCATGTTGCCACCGAAGCTCAAAATTGGCATTGCAGAGCTAAGTATAACGAGTCAGTCGTAAATGTCCTGAAAAACAATCTTCAGCATGCTATCTTGCAGGGTGCCGACCAAGTGAAGGAGGGGTTTGGAGATAGTGAAGTGGATGATGCTGCCTCAAACATTGATCCAAACAATTACGTAAGTGTTCCAGGTGGGACTATAAAATCTTCAACCAGCAGTTTACCAGGTTTCAGGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTTATGCCTTGTAGACATTTATGTTTGTGTAAAGATTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCCGGTATCCAAGTGTACTTGTCCTAAATTATAATCTTACCAAAGCGAAAAAGAAAATCATGTTTTCCTTCTGTCTGTATGATATATACCTGCATTTTTTTCCTTCTAAATCACTAAAGTGAACGTTGAAGTTGCTCATCAAAAAATTGGTGTTTGTCGTACTCGCATGAAGAACAATTACATTCTTTTTTCA

mRNA sequence

TTTTTTTTTTTTAAAAAAAAAGGAACGGAAATCCGTTTGCGTAACCACTACCACCGATTCATTCATTTCATTCTAATAAATTTTCCACTCCCACCGCACCACCTCCCTACAATTTCTCTTCCTCTCTCTCTCTCTCTCTCATATAATTCTTCATCTTCATCATCATCTTCTTCTTCTTCTCGTCGGTTACCTTACCGCTCCAGAAGCCTCCATCCATCTCTCCCCTTTCCTCTATATATTTATGTATGTATATTTCTAGATCCTATTCCTCCTCCTTCATCATTGTCCCCACCTTTGGATTCTACATACTCAATCTTCAAGGATCTGGAGATTATATTTCTTCTCATCTGACACTGCCCAAATGACTTCTTCTTGAGGCAGTGAACCCTCAGTACTGTTGGTATTTTAATTTTCATATTATCTGAAGTTCATCAGTTCAGTTCTGGGGAAAATGTTGGGTGGAAACAATGGCAATGGCTTGCTTCCCGTTTTCCTGGATGAAAATAGAACTCAGTATCAAACTAATGCTTCTGGTCAGCTGCAGTTATTTGGGAATTTTGTCTCGTCTCTCATAGAATTCGCAATTTATGTTGTAGTGCCTGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGTTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAATTAGAAGATTTCTCAAGGCAGCAGAAGCTTCAGATTTCCCTAAATTATGCCTGTCGAGATGATGCTGATCGTTCAGCAAGCATTAGAAACCCAAATGCTGTATCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACCACCGCAAGTGGAAGTATGACAGCTGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGTTCTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAACTTCAGGAAGAACATTTGACAAAGGGTATTAGGGACATGAAGCAAAGACATATGGCTTCCTTTCTTAGTGCGATTGAGAAAGGTATTGGAAAGAAACTACATGAAAAGGATGTTGAAATTGAGAGCATGAACCGTAAAAACAGAGAACTAGTGGAGAGAATAAAGCATGTTGCCACCGAAGCTCAAAATTGGCATTGCAGAGCTAAGTATAACGAGTCAGTCGTAAATGTCCTGAAAAACAATCTTCAGCATGCTATCTTGCAGGGTGCCGACCAAGTGAAGGAGGGGTTTGGAGATAGTGAAGTGGATGATGCTGCCTCAAACATTGATCCAAACAATTACGTAAGTGTTCCAGGTGGGACTATAAAATCTTCAACCAGCAGTTTACCAGGTTTCAGGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTTATGCCTTGTAGACATTTATGTTTGTGTAAAGATTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCCGGTATCCAAGTGTACTTGTCCTAAATTATAATCTTACCAAAGCGAAAAAGAAAATCATGTTTTCCTTCTGTCTGTATGATATATACCTGCATTTTTTTCCTTCTAAATCACTAAAGTGAACGTTGAAGTTGCTCATCAAAAAATTGGTGTTTGTCGTACTCGCATGAAGAACAATTACATTCTTTTTTCA

Coding sequence (CDS)

ATGTTGGGTGGAAACAATGGCAATGGCTTGCTTCCCGTTTTCCTGGATGAAAATAGAACTCAGTATCAAACTAATGCTTCTGGTCAGCTGCAGTTATTTGGGAATTTTGTCTCGTCTCTCATAGAATTCGCAATTTATGTTGTAGTGCCTGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGTTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAATTAGAAGATTTCTCAAGGCAGCAGAAGCTTCAGATTTCCCTAAATTATGCCTGTCGAGATGATGCTGATCGTTCAGCAAGCATTAGAAACCCAAATGCTGTATCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACCACCGCAAGTGGAAGTATGACAGCTGCACCTTCCATCATCTTTTCACTTGGTGATAATATCAGTTCTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAACTTCAGGAAGAACATTTGACAAAGGGTATTAGGGACATGAAGCAAAGACATATGGCTTCCTTTCTTAGTGCGATTGAGAAAGGTATTGGAAAGAAACTACATGAAAAGGATGTTGAAATTGAGAGCATGAACCGTAAAAACAGAGAACTAGTGGAGAGAATAAAGCATGTTGCCACCGAAGCTCAAAATTGGCATTGCAGAGCTAAGTATAACGAGTCAGTCGTAAATGTCCTGAAAAACAATCTTCAGCATGCTATCTTGCAGGGTGCCGACCAAGTGAAGGAGGGGTTTGGAGATAGTGAAGTGGATGATGCTGCCTCAAACATTGATCCAAACAATTACGTAAGTGTTCCAGGTGGGACTATAAAATCTTCAACCAGCAGTTTACCAGGTTTCAGGGAGCACATGATTTGCAGAGCATGCAAGGCAAAAGAGGTTTCCATCCTACTTATGCCTTGTAGACATTTATGTTTGTGTAAAGATTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCCGGTATCCAAGTGTACTTGTCCTAA

Protein sequence

MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNYFGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNYACRDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Homology
[Close]
BLAST of Lsi08G016710 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.5e-22
Identity = 79/244 (32.38%), Postives = 122/244 (50.00%), Query Frame = 0 

Query: 127 SSVTTASGSMTAAPSIIFSLGD--NISSEIDRQKEEFDQYIKL---QEEHLTKGIRDMKQ 186
           SS    + + T   ++   LG   +ISS +++Q+ E D+++ L   Q E +   I + ++
Sbjct: 120 SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRK 179

Query: 187 RHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVV 246
           R   + + AIE+G+ K+L  K+ E E + + N  L ER+K ++ E Q W   A+ NE+  
Sbjct: 180 RQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATA 239

Query: 247 NVLKNNLQHAILQGADQVKEGFG----DSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPG 306
           N L+ NL+H + Q  D V  G G     +E DDA S        S  GG  + +     G
Sbjct: 240 NHLRTNLEHVLAQVKD-VSRGAGLEKNMNEEDDAESCCG-----SSCGGGGEETVRRRVG 299

Query: 307 F----------REHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQ 352
                      R   +CR C  +E  +LL+PCRHLCLC  C   V+ CP+C   K A + 
Sbjct: 300 LEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVH 357

[Close]
BLAST of Lsi08G016710 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-20
Identity = 67/215 (31.16%), Postives = 107/215 (49.77%), Query Frame = 0 

Query: 146 LGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVE 205
           LG ++SS + +   + D+ I    E +   I + ++      + A+E+G+ K L  KD E
Sbjct: 126 LGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDE 185

Query: 206 IESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAILQGADQVKEGFGD 265
           I  + + N  L E++K +  E Q W   A+ NE+ VN L++NLQ  +L   ++ +     
Sbjct: 186 INHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQ-VLAAVERNRWEEPP 245

Query: 266 SEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFREH---------MICRACKAKEVSILL 325
           +  DDA S    N+      G  +     L G  +           +CR+C   E S+LL
Sbjct: 246 TVADDAQSCCGSND-----EGDSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLL 305

Query: 326 MPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           +PCRH+CLC  C   +N CP+C+  KTA + V LS
Sbjct: 306 LPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

[Close]
BLAST of Lsi08G016710 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.2e-18
Identity = 74/248 (29.84%), Postives = 115/248 (46.37%), Query Frame = 0 

Query: 112 VSTGLRLSYDDDEHNSSVTTASGSM-TAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEE 171
           +ST  +  +   + N+ V   S +  +++PS+I    + +S   ++Q+ E D+++  Q E
Sbjct: 75  LSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINV--ELVSQIQNQQQSEIDRFVAQQTE 134

Query: 172 HLTKGIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNW 231
            L   I   +Q       SA++  I KKL EKD EI  +   N  L ER+K +  E Q W
Sbjct: 135 KLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQIW 194

Query: 232 HCRAKYNESVVNVLKNNLQHAILQ-----GADQVKEGFGDSEVDDAASNIDPNNYVSVPG 291
              A+ NE+  N L+ NL   + Q      A  V E   +S       +       +V G
Sbjct: 195 RDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVGG 254

Query: 292 GTIKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDG--LVNVCPVCQLIKT 351
           G                 C+ C  +E S+L++PCRHLCLC  C G  L+  CPVC ++  
Sbjct: 255 G-----------------CKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMN 303

[Close]
BLAST of Lsi08G016710 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.8e-18
Identity = 75/236 (31.78%), Postives = 111/236 (47.03%), Query Frame = 0 

Query: 122 DDEHNSSVTTASGSMTAAPSIIFSLGD-NISSEIDRQKEEFDQYIKLQEEHLTKGIRDMK 181
           D   +S    AS    A      SL D  + S+I +Q  E D+++  Q E L   +   +
Sbjct: 73  DSTFDSDALIASQKRRAIAFSPASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQ 132

Query: 182 QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 241
           +       SA++  I KKL  KD EI  M + N  L ER+K++  E Q W   A+ NE+ 
Sbjct: 133 RTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEAT 192

Query: 242 VNVLKNNLQHAILQGADQVKEGFGD---SEVDDAASNIDPNNYVSVPGGTIKSSTSSLPG 301
            N L++NL+  + Q  D   + F      E DDA S+       S  GG + +  +    
Sbjct: 193 ANNLRSNLEQVLAQVDD--LDAFRRPLVEEADDAESSCG-----SCDGGDVTAVVNG--- 252

Query: 302 FREHMICRACKAKEVSILLMPCRHLCLCKDC--DGLVNVCPVCQLIKTAGIQVYLS 352
                 C+ C     S+L++PCRHLCLC  C    L+  CPVC ++ TA + V +S
Sbjct: 253 -----GCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

[Close]
BLAST of Lsi08G016710 vs. ExPASy Swiss-Prot
Match: O62640 (Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.4e-06
Identity = 24/72 (33.33%), Postives = 37/72 (51.39%), Query Frame = 0 

Query: 280 YVSVPGGTIKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQ 339
           Y+     +  S    L   +E   C+ C  KEVSI+ +PC HL +CKDC   +  CP+C+
Sbjct: 287 YIPTENVSDLSMEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICR 346

Query: 340 LIKTAGIQVYLS 352
                 ++ +LS
Sbjct: 347 GTIKGTVRTFLS 358

[Close]
BLAST of Lsi08G016710 vs. ExPASy TrEMBL
Match: A0A6J1KC06 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492498 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.1e-172
Identity = 312/352 (88.64%), Postives = 327/352 (92.90%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVF DENR QYQTNASGQLQLFG+             VPVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGS-------------VPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNY-ACRDDADRSASIRNPNAVSTGLRLS 120
           FG+EQ+ HMLRPNKRSRE+EDFS QQKLQISLNY AC+DDADRSASIRNPNAVSTGLRLS
Sbjct: 61  FGSEQLAHMLRPNKRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLS 120

Query: 121 YDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDM 180
           YDDDEHNSSVTTASGSM AAPSIIFSLGDNI +EIDRQKEEFDQYIK+QEEHL KGIRDM
Sbjct: 121 YDDDEHNSSVTTASGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDM 180

Query: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNES 240
           KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNES
Sbjct: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNES 240

Query: 241 VVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFR 300
           VVNVLKNNLQHAI QGADQ KEGFGDSEVDDAAS+IDPNNY++V GGT+KSSTS+LPGF+
Sbjct: 241 VVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFK 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           EHMIC+ACKAKEVSILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 EHMICKACKAKEVSILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]
BLAST of Lsi08G016710 vs. ExPASy TrEMBL
Match: A0A1S3B9M9 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC103487699 PE=4 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 313/351 (89.17%), Postives = 322/351 (91.74%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVFLDE RTQYQTNASGQLQLFGN             +PVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQTNASGQLQLFGN-------------LPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNYACRDDADRSASIRNPNAVSTGLRLSY 120
           FGNEQMVHMLRPNKRSRE+EDFSRQQKLQISLNYAC+DDADRS +IRNPN VSTGLRLSY
Sbjct: 61  FGNEQMVHMLRPNKRSREIEDFSRQQKLQISLNYACQDDADRSTTIRNPNPVSTGLRLSY 120

Query: 121 DDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMK 180
           DDDEHNSSVTTASGS+TAAPSIIFSLGDNI +EIDRQKEEFDQYIKLQEEHL KGIRDMK
Sbjct: 121 DDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMK 180

Query: 181 QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240
           QRH ASFLSAIEKGIG KLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV
Sbjct: 181 QRHTASFLSAIEKGIGNKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240

Query: 241 VNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFRE 300
           VNVLKNNLQHAI QGADQ KEGFGDSEVDDAASNIDPNNY+SVPGGTIK STS     +E
Sbjct: 241 VNVLKNNLQHAISQGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKPSTS-----KE 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           +MICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQVYLS
Sbjct: 301 YMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

[Close]
BLAST of Lsi08G016710 vs. ExPASy TrEMBL
Match: A0A6J1HCD7 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111462352 PE=4 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 4.3e-171
Identity = 310/352 (88.07%), Postives = 326/352 (92.61%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVF DENR QYQTNASGQLQLFG+             VPVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGS-------------VPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNY-ACRDDADRSASIRNPNAVSTGLRLS 120
           FG+EQ+ HMLRPNKRSRE+EDFS QQKLQISLNY AC+DDADRSASIRNPNAVSTGLRLS
Sbjct: 61  FGSEQLAHMLRPNKRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLS 120

Query: 121 YDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDM 180
           YDDDEHNSSVTTASGSM  APSIIFSLGDNI +EIDRQKEEFDQYIK+QEEHL KGIRDM
Sbjct: 121 YDDDEHNSSVTTASGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDM 180

Query: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNES 240
           KQRH+ASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNES
Sbjct: 181 KQRHVASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNES 240

Query: 241 VVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFR 300
           VVNVLKNNLQHAI QGADQ KEGFGDSEVDDAAS+IDPNNY++V GGT+KSSTS+LPGF+
Sbjct: 241 VVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFK 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           EHMIC+ACKAKEVSILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 EHMICKACKAKEVSILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]
BLAST of Lsi08G016710 vs. ExPASy TrEMBL
Match: A0A6J1BT85 (BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC111005133 PE=4 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 6.2e-170
Identity = 306/352 (86.93%), Postives = 324/352 (92.05%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGG+NGN LLP+FLDENRTQYQ+NASGQLQLFGNF             PVSCGIEPVNY
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNF-------------PVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNY-ACRDDADRSASIRNPNAVSTGLRLS 120
           FG+EQM HMLRPNKRSRE+EDFSRQQKLQISLNY AC DDADRSA+IRNPNAVSTGLRLS
Sbjct: 61  FGSEQMAHMLRPNKRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLS 120

Query: 121 YDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDM 180
           YDDDEHNSSVTTASGS+TAAPSIIFSLGDNI +EIDRQKEEFDQYIK+QEEHL KGIRDM
Sbjct: 121 YDDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDM 180

Query: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNES 240
           KQRH ASFL+AIEKGIG+KLHEKDVEIE+MNRKNRELVERIK VATEAQNWH RAKYNES
Sbjct: 181 KQRHTASFLNAIEKGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNES 240

Query: 241 VVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFR 300
           VVNVLKNNLQHAI QG DQ KEGFGDSEVDDAAS++DPNNY+SVPGG +KS T +LPGF+
Sbjct: 241 VVNVLKNNLQHAISQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFK 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]
BLAST of Lsi08G016710 vs. ExPASy TrEMBL
Match: A0A0A0LNB0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348840 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 8.1e-170
Identity = 310/351 (88.32%), Postives = 321/351 (91.45%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVFLDE RTQYQ NASGQLQLFGN             +PVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQNNASGQLQLFGN-------------LPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNYACRDDADRSASIRNPNAVSTGLRLSY 120
           FGNEQMV MLRPNKRSRE EDFSRQQKLQISLNYAC+DDA+RS SIRNPNAVSTGLRLSY
Sbjct: 61  FGNEQMVPMLRPNKRSRETEDFSRQQKLQISLNYACQDDANRSTSIRNPNAVSTGLRLSY 120

Query: 121 DDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMK 180
           DDDEHNSSVTTASGS+TAAPSIIFSLGDNI +E+DRQKEEFDQYIK+QEEHL KGIRDMK
Sbjct: 121 DDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEVDRQKEEFDQYIKIQEEHLAKGIRDMK 180

Query: 181 QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240
           QRHMASFLSA+EKGI KKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV
Sbjct: 181 QRHMASFLSAVEKGIEKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240

Query: 241 VNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFRE 300
           VNVLKNNLQHAI QGADQ KEGFGDSEVDDAASNIDPNNYV+VPGGTIK STS     +E
Sbjct: 241 VNVLKNNLQHAISQGADQAKEGFGDSEVDDAASNIDPNNYVNVPGGTIKPSTS-----KE 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           +MICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQVYLS
Sbjct: 301 YMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

[Close]
BLAST of Lsi08G016710 vs. NCBI nr
Match: XP_038884634.1 (probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida])

HSP 1 Score: 639.4 bits (1648), Expect = 1.8e-179
Identity = 323/351 (92.02%), Postives = 332/351 (94.59%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGN             +PVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGN-------------LPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNYACRDDADRSASIRNPNAVSTGLRLSY 120
           FGNEQ+VHMLRPNKRSRE+EDFSRQQKLQISLNYAC+DDADRSASIRNPNAVSTGLRLSY
Sbjct: 61  FGNEQIVHMLRPNKRSREIEDFSRQQKLQISLNYACQDDADRSASIRNPNAVSTGLRLSY 120

Query: 121 DDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMK 180
           DDDEHNSSVTTASGSMTAAPSII SLGDNI +EIDRQKEEFDQYIKLQEEHL KGIRDMK
Sbjct: 121 DDDEHNSSVTTASGSMTAAPSIILSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMK 180

Query: 181 QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240
           QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV
Sbjct: 181 QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240

Query: 241 VNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFRE 300
           VNVLKNNLQHAI QGADQ KEGFGDSEVDDAASNIDPNNY+SVPGGTIKSSTS+LPGF+E
Sbjct: 241 VNVLKNNLQHAISQGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKSSTSNLPGFKE 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQV+LS
Sbjct: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVFLS 338

[Close]
BLAST of Lsi08G016710 vs. NCBI nr
Match: XP_022997618.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.1 BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_023545645.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545646.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545647.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613.6 bits (1581), Expect = 1.1e-171
Identity = 312/352 (88.64%), Postives = 327/352 (92.90%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVF DENR QYQTNASGQLQLFG+             VPVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGS-------------VPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNY-ACRDDADRSASIRNPNAVSTGLRLS 120
           FG+EQ+ HMLRPNKRSRE+EDFS QQKLQISLNY AC+DDADRSASIRNPNAVSTGLRLS
Sbjct: 61  FGSEQLAHMLRPNKRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLS 120

Query: 121 YDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDM 180
           YDDDEHNSSVTTASGSM AAPSIIFSLGDNI +EIDRQKEEFDQYIK+QEEHL KGIRDM
Sbjct: 121 YDDDEHNSSVTTASGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDM 180

Query: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNES 240
           KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNES
Sbjct: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNES 240

Query: 241 VVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFR 300
           VVNVLKNNLQHAI QGADQ KEGFGDSEVDDAAS+IDPNNY++V GGT+KSSTS+LPGF+
Sbjct: 241 VVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFK 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           EHMIC+ACKAKEVSILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 EHMICKACKAKEVSILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]
BLAST of Lsi08G016710 vs. NCBI nr
Match: XP_008444346.1 (PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo])

HSP 1 Score: 612.5 bits (1578), Expect = 2.3e-171
Identity = 313/351 (89.17%), Postives = 322/351 (91.74%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVFLDE RTQYQTNASGQLQLFGN             +PVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFLDEKRTQYQTNASGQLQLFGN-------------LPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNYACRDDADRSASIRNPNAVSTGLRLSY 120
           FGNEQMVHMLRPNKRSRE+EDFSRQQKLQISLNYAC+DDADRS +IRNPN VSTGLRLSY
Sbjct: 61  FGNEQMVHMLRPNKRSREIEDFSRQQKLQISLNYACQDDADRSTTIRNPNPVSTGLRLSY 120

Query: 121 DDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMK 180
           DDDEHNSSVTTASGS+TAAPSIIFSLGDNI +EIDRQKEEFDQYIKLQEEHL KGIRDMK
Sbjct: 121 DDDEHNSSVTTASGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMK 180

Query: 181 QRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240
           QRH ASFLSAIEKGIG KLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV
Sbjct: 181 QRHTASFLSAIEKGIGNKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESV 240

Query: 241 VNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFRE 300
           VNVLKNNLQHAI QGADQ KEGFGDSEVDDAASNIDPNNY+SVPGGTIK STS     +E
Sbjct: 241 VNVLKNNLQHAISQGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKPSTS-----KE 300

Query: 301 HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           +MICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQVYLS
Sbjct: 301 YMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVYLS 333

[Close]
BLAST of Lsi08G016710 vs. NCBI nr
Match: KAG6598221.1 (putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.1 bits (1577), Expect = 3.1e-171
Identity = 311/352 (88.35%), Postives = 326/352 (92.61%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVF DENR QYQTNASGQLQLFG+             VPVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGS-------------VPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNY-ACRDDADRSASIRNPNAVSTGLRLS 120
           FG+EQ+ HMLRPNKRSRE+EDFS QQKLQISLNY AC+DDADRSASIRNPNAVSTGLRLS
Sbjct: 61  FGSEQLAHMLRPNKRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLS 120

Query: 121 YDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDM 180
           YDDDEHNSSVTTASGSM  APSIIFSLGDNI +EIDRQKEEFDQYIK+QEEHL KGIRDM
Sbjct: 121 YDDDEHNSSVTTASGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDM 180

Query: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNES 240
           KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNES
Sbjct: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNES 240

Query: 241 VVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFR 300
           VVNVLKNNLQHAI QGADQ KEGFGDSEVDDAAS+IDPNNY++V GGT+KSSTS+LPGF+
Sbjct: 241 VVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFK 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           EHMIC+ACKAKEVSILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 EHMICKACKAKEVSILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]
BLAST of Lsi08G016710 vs. NCBI nr
Match: XP_022961638.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961640.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata])

HSP 1 Score: 610.5 bits (1573), Expect = 8.9e-171
Identity = 310/352 (88.07%), Postives = 326/352 (92.61%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVNY 60
           MLGGNNGNGLLPVF DENR QYQTNASGQLQLFG+             VPVSCGIEPVNY
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGS-------------VPVSCGIEPVNY 60

Query: 61  FGNEQMVHMLRPNKRSRELEDFSRQQKLQISLNY-ACRDDADRSASIRNPNAVSTGLRLS 120
           FG+EQ+ HMLRPNKRSRE+EDFS QQKLQISLNY AC+DDADRSASIRNPNAVSTGLRLS
Sbjct: 61  FGSEQLAHMLRPNKRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLS 120

Query: 121 YDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDM 180
           YDDDEHNSSVTTASGSM  APSIIFSLGDNI +EIDRQKEEFDQYIK+QEEHL KGIRDM
Sbjct: 121 YDDDEHNSSVTTASGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDM 180

Query: 181 KQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNES 240
           KQRH+ASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIK VATEAQNWHCRAKYNES
Sbjct: 181 KQRHVASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNES 240

Query: 241 VVNVLKNNLQHAILQGADQVKEGFGDSEVDDAASNIDPNNYVSVPGGTIKSSTSSLPGFR 300
           VVNVLKNNLQHAI QGADQ KEGFGDSEVDDAAS+IDPNNY++V GGT+KSSTS+LPGF+
Sbjct: 241 VVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFK 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           EHMIC+ACKAKEVSILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 EHMICKACKAKEVSILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

[Close]
BLAST of Lsi08G016710 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 362.5 bits (929), Expect = 3.9e-100
Identity = 199/364 (54.67%), Postives = 249/364 (68.41%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTN-ASGQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVN 60
           ML GNNGN   PVF++EN+ QYQTN  S QL L GN                 C ++PVN
Sbjct: 1   MLSGNNGNTAPPVFMNENQLQYQTNLRSNQLHLLGNMGG-------------GCTVDPVN 60

Query: 61  YFGNEQMVHMLRPNKRSRELEDFS-----RQQKLQISLNYACRDDADRSASIRNPNAVST 120
           YF N+ +V M+RPNKR RE E  S     +QQKLQ+SLNY   + + R   +   N VST
Sbjct: 61  YFANDNLVPMIRPNKRGREAESISNNIQRQQQKLQMSLNYNYNNTSVRE-EVPKENLVST 120

Query: 121 GLRLSYDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTK 180
           GLRLSYDDDEHNSSVT+ASGS+ AA  I  SL D++  ++ RQK+EFDQ+IK+Q   + K
Sbjct: 121 GLRLSYDDDEHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAK 180

Query: 181 GIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRA 240
           G+RDMKQRH+ASFL+ +EKG+ KKL EKD EI  MN+KN+ELVERIK VA EAQNWH RA
Sbjct: 181 GVRDMKQRHIASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRA 240

Query: 241 KYNESVVNVLKNNLQHA------ILQGADQVKEGFGDSEVDDAASN-IDPNNYVSVPGGT 300
           KYNESVVNVLK NLQ A      ++  ADQ KEGFGDSE+DDAAS+ IDPNN        
Sbjct: 241 KYNESVVNVLKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNN-------- 300

Query: 301 IKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQ 352
              + ++  G  + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +Q
Sbjct: 301 ---NNNNNMGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQ 339

[Close]
BLAST of Lsi08G016710 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.7e-85
Identity = 182/367 (49.59%), Postives = 237/367 (64.58%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNAS-GQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVN 60
           MLGGNN N +  V +++++ +YQ+N S  QL L G              +   C I+PVN
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLG-------------TMRAGCTIDPVN 60

Query: 61  YFGNEQMVHMLRPNK-RSRELED---FSRQQKLQISLNYAC-RDDADRSASIRNPNAVST 120
           YF N+ +  M+R N  R RE E+     RQQKLQISLNY    ++      +   N VST
Sbjct: 61  YFANDNLAPMMRLNSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVST 120

Query: 121 GLRLSYDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTK 180
           GLRLSYDDDE NSSVT+A+GS+T    +  SLGDNI  +++RQ +E DQ+IK + + + K
Sbjct: 121 GLRLSYDDDERNSSVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAK 180

Query: 181 GIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRA 240
           G+RD+KQRH+ SF++A+EK + KKL EKD EIESMN+KNRELV++IK VA EAQNWH +A
Sbjct: 181 GVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKA 240

Query: 241 KYNESVVNVLKNNLQHAILQGAD------------QVKEGFGDSEVDDAASNIDPNNYVS 300
           KYNESVVN LK NLQ  +  G D            Q+KEGFGDSE+DD A++    NY++
Sbjct: 241 KYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLN 300

Query: 301 VPGGTIKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIK 350
           +PG     ST         M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +K
Sbjct: 301 IPG---MPSTG--------MRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLK 338

[Close]
BLAST of Lsi08G016710 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.7e-85
Identity = 182/367 (49.59%), Postives = 237/367 (64.58%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNAS-GQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVN 60
           MLGGNN N +  V +++++ +YQ+N S  QL L G              +   C I+PVN
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLG-------------TMRAGCTIDPVN 60

Query: 61  YFGNEQMVHMLRPNK-RSRELED---FSRQQKLQISLNYAC-RDDADRSASIRNPNAVST 120
           YF N+ +  M+R N  R RE E+     RQQKLQISLNY    ++      +   N VST
Sbjct: 61  YFANDNLAPMMRLNSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVST 120

Query: 121 GLRLSYDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTK 180
           GLRLSYDDDE NSSVT+A+GS+T    +  SLGDNI  +++RQ +E DQ+IK + + + K
Sbjct: 121 GLRLSYDDDERNSSVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAK 180

Query: 181 GIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRA 240
           G+RD+KQRH+ SF++A+EK + KKL EKD EIESMN+KNRELV++IK VA EAQNWH +A
Sbjct: 181 GVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKA 240

Query: 241 KYNESVVNVLKNNLQHAILQGAD------------QVKEGFGDSEVDDAASNIDPNNYVS 300
           KYNESVVN LK NLQ  +  G D            Q+KEGFGDSE+DD A++    NY++
Sbjct: 241 KYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLN 300

Query: 301 VPGGTIKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIK 350
           +PG     ST         M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +K
Sbjct: 301 IPG---MPSTG--------MRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLK 338

[Close]
BLAST of Lsi08G016710 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 313.2 bits (801), Expect = 2.7e-85
Identity = 182/367 (49.59%), Postives = 237/367 (64.58%), Query Frame = 0 

Query: 1   MLGGNNGNGLLPVFLDENRTQYQTNAS-GQLQLFGNFVSSLIEFAIYVVVPVSCGIEPVN 60
           MLGGNN N +  V +++++ +YQ+N S  QL L G              +   C I+PVN
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLG-------------TMRAGCTIDPVN 60

Query: 61  YFGNEQMVHMLRPNK-RSRELED---FSRQQKLQISLNYAC-RDDADRSASIRNPNAVST 120
           YF N+ +  M+R N  R RE E+     RQQKLQISLNY    ++      +   N VST
Sbjct: 61  YFANDNLAPMMRLNSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVST 120

Query: 121 GLRLSYDDDEHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTK 180
           GLRLSYDDDE NSSVT+A+GS+T    +  SLGDNI  +++RQ +E DQ+IK + + + K
Sbjct: 121 GLRLSYDDDERNSSVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAK 180

Query: 181 GIRDMKQRHMASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRA 240
           G+RD+KQRH+ SF++A+EK + KKL EKD EIESMN+KNRELV++IK VA EAQNWH +A
Sbjct: 181 GVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKA 240

Query: 241 KYNESVVNVLKNNLQHAILQGAD------------QVKEGFGDSEVDDAASNIDPNNYVS 300
           KYNESVVN LK NLQ  +  G D            Q+KEGFGDSE+DD A++    NY++
Sbjct: 241 KYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLN 300

Query: 301 VPGGTIKSSTSSLPGFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIK 350
           +PG     ST         M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +K
Sbjct: 301 IPG---MPSTG--------MRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLK 338

[Close]
BLAST of Lsi08G016710 vs. TAIR 10
Match: AT1G10650.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.0e-84
Identity = 168/295 (56.95%), Postives = 211/295 (71.53%), Query Frame = 0 

Query: 69  MLRPNKRSRELEDFS-----RQQKLQISLNYACRDDADRSASIRNPNAVSTGLRLSYDDD 128
           M+RPNKR RE E  S     +QQKLQ+SLNY   + + R   +   N VSTGLRLSYDDD
Sbjct: 1   MIRPNKRGREAESISNNIQRQQQKLQMSLNYNYNNTSVRE-EVPKENLVSTGLRLSYDDD 60

Query: 129 EHNSSVTTASGSMTAAPSIIFSLGDNISSEIDRQKEEFDQYIKLQEEHLTKGIRDMKQRH 188
           EHNSSVT+ASGS+ AA  I  SL D++  ++ RQK+EFDQ+IK+Q   + KG+RDMKQRH
Sbjct: 61  EHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRH 120

Query: 189 MASFLSAIEKGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNV 248
           +ASFL+ +EKG+ KKL EKD EI  MN+KN+ELVERIK VA EAQNWH RAKYNESVVNV
Sbjct: 121 IASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNV 180

Query: 249 LKNNLQHA------ILQGADQVKEGFGDSEVDDAASN-IDPNNYVSVPGGTIKSSTSSLP 308
           LK NLQ A      ++  ADQ KEGFGDSE+DDAAS+ IDPNN           + ++  
Sbjct: 181 LKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNN-----------NNNNNM 240

Query: 309 GFREHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 352
           G  + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 241 GIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 283

The following BLAST results are available for this feature:
BLAST of Lsi08G016710 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4IDI62.5e-2232.38Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9LDD11.0e-2031.16Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
O818512.2e-1829.84E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q9FHE44.8e-1831.78BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
O626409.4e-0633.33Putative inhibitor of apoptosis OS=Sus scrofa OX=9823 GN=PIAP PE=2 SV=1[more]
BLAST of Lsi08G016710 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KC065.1e-17288.64BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S3B9M91.1e-17189.17BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC10348769... [more]
A0A6J1HCD74.3e-17188.07BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1BT856.2e-17086.93BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0LNB08.1e-17088.32RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348840 P... [more]
BLAST of Lsi08G016710 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038884634.11.8e-17992.02probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida][more]
XP_022997618.11.1e-17188.64BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.... [more]
XP_008444346.12.3e-17189.17PREDICTED: BOI-related E3 ubiquitin-protein ligase 1 [Cucumis melo][more]
KAG6598221.13.1e-17188.35putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrospe... [more]
XP_022961638.18.9e-17188.07BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 B... [more]
BLAST of Lsi08G016710 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G10650.13.9e-10054.67SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.12.7e-8549.59SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.22.7e-8549.59SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.32.7e-8549.59SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.23.0e-8456.95SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 199..226
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 302..339
e-value: 5.1E-7
score: 29.4
NoneNo IPR availablePANTHERPTHR42647:SF9S-RIBONUCLEASE BINDING PROTEIN SBP1-RELATEDcoord: 4..350
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 8..351
e-value: 9.3E-95
score: 316.1
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 4..350
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 290..351
e-value: 5.5E-9
score: 37.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 304..339
score: 8.963311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G016710.1Lsi08G016710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity