Lsi08G016330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G016330
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cysteine proteinases superfamily protein
Location	chr08: 24228181 .. 24228771 (-)
RNA-Seq Expression	Lsi08G016330
Synteny	Lsi08G016330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTGTCAATGACATCAAAGATCAAGGCTCATGTGGTATAATTTTGAATTTTGGTTGTGTAAAAGAGGTTGTTTGCAGGAAGTTGTTGGGCATTTGCAGACGTTGTTAAAATGAGTTGTCAATGACATCAAAGATCAAAATCCCTAGATGTTGTTAATTGTGATTTTAATGACGGAGGTTGCGAGGATTCTATAACTATGCTTTTAAATGCAAAATGATGGGATCACAACGGATAAAAACTATCCCTACTATGCCCAAAATGATTATTGCCGCCCATCGAGAGTGAGTGTGGAAGTAAATTAAACCCTAAATTAATATAAATGGAAGCTAATTAAATATATATTTTGATTTGACTAAGTGACAATTGTGGTGGTAGTTGGATACGGAATCGAGGAAGACGGAACAGATTATTGGATCATAAGTAACTCATGGAGAGTTGGATGGGAATTGGAAGGTTATATGAAGATGCAACGAGGAGTGGAGAATCCAGGAGGTGTATATGGATTGGCAATGAATTCTTCATATCCCGTAAAGCATAGCATCCACGCAGCTTCCTATTCTATTTCCATTCTTTTTAGCATTCTTTAG

mRNA sequence

ATGATGACAATTGTGGTGGTAGTTGGATACGGAATCGAGGAAGACGGAACAGATTATTGGATCATAAGTAACTCATGGAGAGTTGGATGGGAATTGGAAGGTTATATGAAGATGCAACGAGGAGTGGAGAATCCAGGAGGTGTATATGGATTGGCAATGAATTCTTCATATCCCGTAAAGCATAGCATCCACGCAGCTTCCTATTCTATTTCCATTCTTTTTAGCATTCTTTAG

Coding sequence (CDS)

ATGATGACAATTGTGGTGGTAGTTGGATACGGAATCGAGGAAGACGGAACAGATTATTGGATCATAAGTAACTCATGGAGAGTTGGATGGGAATTGGAAGGTTATATGAAGATGCAACGAGGAGTGGAGAATCCAGGAGGTGTATATGGATTGGCAATGAATTCTTCATATCCCGTAAAGCATAGCATCCACGCAGCTTCCTATTCTATTTCCATTCTTTTTAGCATTCTTTAG

Protein sequence

MMTIVVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIHAASYSISILFSIL

Homology

[Close]

BLAST of Lsi08G016330 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-12
Identity = 31/58 (53.45%), Postives = 42/58 (72.41%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHS 63
           V VVGYG   DGT YWI+ NSW   W  +GY++MQRG+ +  G+ G+AM +SYP+K+S
Sbjct: 288 VAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLKNS 345

[Close]

BLAST of Lsi08G016330 vs. ExPASy Swiss-Prot
Match: P43156 (Thiol protease SEN102 OS=Hemerocallis sp. OX=29711 GN=SEN102 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.3e-12
Identity = 31/65 (47.69%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIH 64
           V +VGYG   DGT YWI+ NSW   W   GY++MQRG+ +  G  G+AM +SYP+K S +
Sbjct: 291 VAIVGYGATRDGTKYWIVKNSWGEEWGESGYIRMQRGISDKRGKCGIAMEASYPIKTSAN 350

Query: 65  AASYS 70
             + S
Sbjct: 351 PKNSS 355

[Close]

BLAST of Lsi08G016330 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.6e-12
Identity = 31/67 (46.27%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIH 64
           V +VGYG   DGT+YWI+ NSW   W  +GY++MQR +    G+ G+AM +SYP+K+S  
Sbjct: 290 VAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIKNSSD 349

Query: 65  AASYSIS 72
             + S+S
Sbjct: 350 NPTGSLS 356

[Close]

BLAST of Lsi08G016330 vs. ExPASy Swiss-Prot
Match: Q9SUT0 (Probable cysteine protease RDL4 OS=Arabidopsis thaliana OX=3702 GN=RDL4 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 9.6e-12
Identity = 34/67 (50.75%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIH 64
           VVVVGYG  E+G DYW++ NS  + W   GYMKM R + NP G+ G+AM +SYP+K+S  
Sbjct: 299 VVVVGYG-TENGRDYWLVKNSRGITWGEAGYMKMARNIANPRGLCGIAMRASYPLKNSFS 358

Query: 65  AASYSIS 72
               SI+
Sbjct: 359 TDKSSIA 364

[Close]

BLAST of Lsi08G016330 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.6e-11
Identity = 28/58 (48.28%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHS 63
           V +VGYG   DGT YW + NSW   W  +GY++M+RG+ +  G+ G+AM +SYP+K S
Sbjct: 288 VAIVGYGTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIKKS 345

[Close]

BLAST of Lsi08G016330 vs. ExPASy TrEMBL
Match: A0A1S3BA70 (vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.2e-14
Identity = 35/56 (62.50%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVK 61
           VV +GYG  EDGTDYWI+ NSW VGW  +GY++M+RGVE P G+ G+AM +SYP+K
Sbjct: 289 VVAIGYGTTEDGTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIK 344

[Close]

BLAST of Lsi08G016330 vs. ExPASy TrEMBL
Match: A0A0A0LMU4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 5.3e-13
Identity = 33/57 (57.89%), Postives = 45/57 (78.95%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKH 62
           VV +GYG  EDGTDYW++ NSW VGW  +GY++M+RGVE   G+ G+AM +SYP+K+
Sbjct: 289 VVAIGYGTTEDGTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

[Close]

BLAST of Lsi08G016330 vs. ExPASy TrEMBL
Match: Q84M27 (Cysteine protease-3 OS=Helianthus annuus OX=4232 GN=scp3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-12
Identity = 34/58 (58.62%), Postives = 45/58 (77.59%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHS 63
           V +VGYG  +DGT+YWI+ NSW  GW  +GY++MQRGV  P G+ GLAM++SYP+K S
Sbjct: 287 VAIVGYGTTQDGTNYWIVRNSWGTGWGEQGYVRMQRGVNVPEGLCGLAMDASYPIKAS 344

[Close]

BLAST of Lsi08G016330 vs. ExPASy TrEMBL
Match: A0A6J1KIL0 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-12
Identity = 33/56 (58.93%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVK 61
           VVV+GYG  + GTDYW + NSW VGW  +GY++M+RGVE+P G+ G+AM +SYP+K
Sbjct: 289 VVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 344

[Close]

BLAST of Lsi08G016330 vs. ExPASy TrEMBL
Match: A0A7C9DPJ4 (Phospholipase A(2) OS=Opuntia streptacantha OX=393608 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-12
Identity = 36/60 (60.00%), Postives = 44/60 (73.33%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIH 64
           VVVVGYGI+EDG DYWI+ NSW  GW  +GY+K+QR      G  G+AM  SYPVKHS++
Sbjct: 41  VVVVGYGIDEDGLDYWIVRNSWGKGWGEDGYIKLQRNSHTFTGKCGIAMQPSYPVKHSLN 100

[Close]

BLAST of Lsi08G016330 vs. NCBI nr
Match: XP_038896226.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 97.1 bits (240), Expect = 7.3e-17
Identity = 43/57 (75.44%), Postives = 46/57 (80.70%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKH 62
           VVVVGYG EEDGTDYWII NSW   W LEGYMKMQRG + P GV GLAMN SYP+K+
Sbjct: 337 VVVVGYGTEEDGTDYWIIKNSWGTNWGLEGYMKMQRGAQQPEGVCGLAMNPSYPIKY 393

[Close]

BLAST of Lsi08G016330 vs. NCBI nr
Match: XP_038885798.1 (vignain-like [Benincasa hispida])

HSP 1 Score: 91.7 bits (226), Expect = 3.1e-15
Identity = 41/57 (71.93%), Postives = 43/57 (75.44%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKH 62
           VVVVGYG EEDG DYWII NSW   W LEGYMKMQRG   P  V GLAMN SYP+K+
Sbjct: 362 VVVVGYGTEEDGADYWIIKNSWGTNWGLEGYMKMQRGARQPEDVCGLAMNPSYPIKY 418

[Close]

BLAST of Lsi08G016330 vs. NCBI nr
Match: XP_038885064.1 (LOW QUALITY PROTEIN: vignain-like [Benincasa hispida])

HSP 1 Score: 87.4 bits (215), Expect = 5.8e-14
Identity = 36/56 (64.29%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVK 61
           VV +GYG  EDGTDYWI+ NSW VGW  EGY++M+RGVE P G+ G+AM +SYP+K
Sbjct: 318 VVAIGYGTTEDGTDYWIVRNSWGVGWGEEGYVRMKRGVEQPEGLCGIAMEASYPIK 373

[Close]

BLAST of Lsi08G016330 vs. NCBI nr
Match: XP_008444390.1 (PREDICTED: vignain-like [Cucumis melo])

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-13
Identity = 35/56 (62.50%), Postives = 45/56 (80.36%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVK 61
           VV +GYG  EDGTDYWI+ NSW VGW  +GY++M+RGVE P G+ G+AM +SYP+K
Sbjct: 289 VVAIGYGTTEDGTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIK 344

[Close]

BLAST of Lsi08G016330 vs. NCBI nr
Match: XP_004142960.1 (vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis sativus])

HSP 1 Score: 83.2 bits (204), Expect = 1.1e-12
Identity = 33/57 (57.89%), Postives = 45/57 (78.95%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKH 62
           VV +GYG  EDGTDYW++ NSW VGW  +GY++M+RGVE   G+ G+AM +SYP+K+
Sbjct: 289 VVAIGYGTTEDGTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

[Close]

BLAST of Lsi08G016330 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 1.0e-13
Identity = 31/58 (53.45%), Postives = 42/58 (72.41%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHS 63
           V VVGYG   DGT YWI+ NSW   W  +GY++MQRG+ +  G+ G+AM +SYP+K+S
Sbjct: 288 VAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLKNS 345

[Close]

BLAST of Lsi08G016330 vs. TAIR 10
Match: AT4G11310.1 (Papain family cysteine protease )

HSP 1 Score: 70.5 bits (171), Expect = 6.8e-13
Identity = 34/67 (50.75%), Postives = 45/67 (67.16%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIH 64
           VVVVGYG  E+G DYW++ NS  + W   GYMKM R + NP G+ G+AM +SYP+K+S  
Sbjct: 299 VVVVGYG-TENGRDYWLVKNSRGITWGEAGYMKMARNIANPRGLCGIAMRASYPLKNSFS 358

Query: 65  AASYSIS 72
               SI+
Sbjct: 359 TDKSSIA 364

[Close]

BLAST of Lsi08G016330 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 69.7 bits (169), Expect = 1.2e-12
Identity = 29/56 (51.79%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVK 61
           V++VGYG  ++G DYWI+ NSW   W ++G+M MQR  EN  GV G+ M +SYP+K
Sbjct: 280 VLIVGYG-SQNGVDYWIVKNSWGKSWGMDGFMHMQRNTENSDGVCGINMLASYPIK 334

[Close]

BLAST of Lsi08G016330 vs. TAIR 10
Match: AT4G11320.1 (Papain family cysteine protease )

HSP 1 Score: 68.9 bits (167), Expect = 2.0e-12
Identity = 34/67 (50.75%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHSIH 64
           VVVVGYG  E+G DYWI+ NS    W   GYMKM R + NP G+ G+AM +SYP+K+S  
Sbjct: 306 VVVVGYG-TENGRDYWIVKNSRGDTWGEAGYMKMARNIANPRGLCGIAMRASYPLKNSFS 365

Query: 65  AASYSIS 72
               S++
Sbjct: 366 TDKVSVA 371

[Close]

BLAST of Lsi08G016330 vs. TAIR 10
Match: AT3G19400.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 68.6 bits (166), Expect = 2.6e-12
Identity = 32/58 (55.17%), Postives = 39/58 (67.24%), Query Frame = 0


Query: 5   VVVVGYGIEEDGTDYWIISNSWRVGWELEGYMKMQRGVENPGGVYGLAMNSSYPVKHS 63
           VVVVGYG    G DYWII NSW + W   GY+K+QR +++P G  G+AM  SYP K S
Sbjct: 295 VVVVGYG-STSGEDYWIIRNSWGLNWGDSGYVKLQRNIDDPFGKCGIAMMPSYPTKSS 351

The following BLAST results are available for this feature:

BLAST of Lsi08G016330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGR9	1.5e-12	53.45	KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ...	[more]
P43156	4.3e-12	47.69	Thiol protease SEN102 OS=Hemerocallis sp. OX=29711 GN=SEN102 PE=2 SV=1	[more]
P12412	5.6e-12	46.27	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
Q9SUT0	9.6e-12	50.75	Probable cysteine protease RDL4 OS=Arabidopsis thaliana OX=3702 GN=RDL4 PE=2 SV=...	[more]
O65039	1.6e-11	48.28	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]

BLAST of Lsi08G016330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BA70	6.2e-14	62.50	vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1	[more]
A0A0A0LMU4	5.3e-13	57.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1	[more]
Q84M27	1.2e-12	58.62	Cysteine protease-3 OS=Helianthus annuus OX=4232 GN=scp3 PE=2 SV=1	[more]
A0A6J1KIL0	1.5e-12	58.93	vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1	[more]
A0A7C9DPJ4	1.5e-12	60.00	Phospholipase A(2) OS=Opuntia streptacantha OX=393608 PE=3 SV=1	[more]

BLAST of Lsi08G016330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896226.1	7.3e-17	75.44	vignain-like [Benincasa hispida]	[more]
XP_038885798.1	3.1e-15	71.93	vignain-like [Benincasa hispida]	[more]
XP_038885064.1	5.8e-14	64.29	LOW QUALITY PROTEIN: vignain-like [Benincasa hispida]	[more]
XP_008444390.1	1.3e-13	62.50	PREDICTED: vignain-like [Cucumis melo]	[more]
XP_004142960.1	1.1e-12	57.89	vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis s...	[more]

BLAST of Lsi08G016330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50260.1	1.0e-13	53.45	Cysteine proteinases superfamily protein	[more]
AT4G11310.1	6.8e-13	50.75	Papain family cysteine protease	[more]
AT1G09850.1	1.2e-12	51.79	xylem bark cysteine peptidase 3	[more]
AT4G11320.1	2.0e-12	50.75	Papain family cysteine protease	[more]
AT3G19400.1	2.6e-12	55.17	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 4..58 e-value: 1.3E-12 score: 48.3
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 2..63 e-value: 9.5E-15 score: 56.9
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 5..67
None	No IPR available	PANTHER	PTHR12411:SF533	CYSTEINE ENDOPEPTIDASE	coord: 5..67
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 5..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G016330.1	Lsi08G016330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
cellular_component	GO:0016020	membrane
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity