Lsi08G016180 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G016180 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.4e-61
Identity = 153/437 (35.01%), Postives = 242/437 (55.38%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           M  ++ I+SEELI+PS+PTP  L+ + LS +DQ+ +  + P + FY         +LD A
Sbjct: 1   MAPQMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFY---PNQLDSNLDRA 60

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPD 120
              +N L+ +L  VL+ + PLAGR N  SS ++CND+GVPF EARV+SQL++ ++    D
Sbjct: 61  QRSEN-LKRSLSTVLTQFYPLAGRININSS-VDCNDSGVPFLEARVHSQLSEAIKNVAID 120

Query: 121 DLNRLFPVEMDRFNEEL-------MAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNW 180
           +LN+  P +     EE        +AV+ + F CGG A+  CISHKIADA+++ +  N+W
Sbjct: 121 ELNQYLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSW 180

Query: 181 AAMARGVKDVFKPHVE-GAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAE 240
            A  +   D+ +P+ + G+  FPP             N V +RFVF+  KLEA++A+ A 
Sbjct: 181 TATCQEETDIVQPNFDLGSHHFPPMESIPAPEFLPDENIVMKRFVFDKEKLEALKAQLAS 240

Query: 241 NEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDA 300
           +   +  +  SR++ + A ++ +F+   + +    N +  +    VNLR +MNPP PQ A
Sbjct: 241 SATEVKNS--SRVQIVIAVIWKQFIDVTRAKFDTKNKL--VAAQAVNLRSRMNPPFPQSA 300

Query: 301 FGNYYFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIA 360
            GN     M +   E   +D++   LV  L+    KI+ E  K       +L + V  + 
Sbjct: 301 MGN--IATMAYAVAE---EDKDFSDLVGPLKTSLAKIDDEHVK-------ELQKGVTYLD 360

Query: 361 SKVVNGEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIV 420
            +    E+ S  FSS CR   YD+DFGWG+PV V    +  KNLV  +D++++D ++A +
Sbjct: 361 YEAEPQELFS--FSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWI 414

Query: 421 HLEEQYMNKLEADELFL 430
            + E  M+ L +D L L
Sbjct: 421 SMAEDEMSMLSSDFLSL 414

BLAST of Lsi08G016180 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.3e-48
Identity = 141/451 (31.26%), Postives = 232/451 (51.44%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           + +E + +S+ LI+PS+PTP  L RY LS+ DQ          +FY + DG     ++ +
Sbjct: 6   ISVETETLSKTLIKPSSPTPQSLSRYNLSYNDQNIYQTCVSVGFFYENPDG-----IEIS 65

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQF---A 120
              + +L+++L   L  Y P AG+    + +I+CND G+ F E R+  ++ D++++   +
Sbjct: 66  -TIREQLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVEVRIRCRMNDILKYELRS 125

Query: 121 QPDDLNRLFPVEMDRFNEELMA-VQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAM 180
              DL  + P  +   +E+  A VQ + F CGG+AVA  ISHK+AD  T+ S   +WAA 
Sbjct: 126 YARDL--VLPKRVTVGSEDTTAIVQLSHFDCGGLAVAFGISHKVADGGTIASFMKDWAAS 185

Query: 181 A---RGVKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAEN 240
           A        V  P +    IFP +           +N V + F+F P  +E +++K  E 
Sbjct: 186 ACYLSSSHHVPTPLLVSDSIFPRQDNIICEQFPTSKNCVEKTFIFPPEAIEKLKSKAVE- 245

Query: 241 EAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPN---DMSYLINYTVNLRPKMNPPLPQ 300
                  +P+R+E L+AF+      A K  +   N    + + +   +NLRP +   LPQ
Sbjct: 246 ---FGIEKPTRVEVLTAFLSRCATVAGKSAAKNNNCGQSLPFPVLQAINLRPILE--LPQ 305

Query: 301 DAFGN---YYFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKK--FLNEDEDLM 360
           ++ GN    YF+  I    +  N+ E    ++ +LR+E  KI     +K  ++ + E+ +
Sbjct: 306 NSVGNLVSIYFSRTI-KENDYLNEKEYTKLVINELRKEKQKIKNLSREKLTYVAQMEEFV 365

Query: 361 RTVKEIASKVVNGEIISCAFS-SICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSK- 420
           +++KE    + N   I    S S CRFP YDVDFGWG+P+WV     + KN V  +D   
Sbjct: 366 KSLKEF--DISNFLDIDAYLSDSWCRFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMDYPF 425

Query: 421 -DDDAIDAIVHLEEQYMNKLEADELFLEYAT 434
            DD  I+AIV  E++ M+  E +E  L++ +
Sbjct: 426 GDDYGIEAIVSFEQEKMSAFEKNEQLLQFVS 438

BLAST of Lsi08G016180 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 3.3e-48
Identity = 140/441 (31.75%), Postives = 231/441 (52.38%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           M  +++ +SEELI PS+PTP  L+ Y +S +DQ+ +  + P + FY         +LD A
Sbjct: 1   MAPQMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFY---PNPLDSNLDPA 60

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLA----DVVQF 120
                 L+ +L  VL+H+ PLAGR N  SS ++CND+GVPF EARV +QL+    +VV+ 
Sbjct: 61  QT-SQHLKQSLSKVLTHFYPLAGRINVNSS-VDCNDSGVPFVEARVQAQLSQAIQNVVEL 120

Query: 121 AQPDDL--NRLFPVEMDRFNEEL-MAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNW 180
            + D    +  +P      NE++ +AV+ + F CGG A+   +SHKIAD +++ +  N W
Sbjct: 121 EKLDQYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAW 180

Query: 181 AAMARGVKDVFKPHVE-GAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAE 240
            A  RG  ++  P+ +  A+ FPP   + +  +    N V +RFVF+  K+ A+R   A+
Sbjct: 181 TATCRGETEIVLPNFDLAARHFPPVDNTPSPELVPDENVVMKRFVFDKEKIGALR---AQ 240

Query: 241 NEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDA 300
             +   +   SR++ + A+++   +   + +    N   +++   VNLR +MNPPLP  A
Sbjct: 241 ASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKN--KFVVVQAVNLRSRMNPPLPHYA 300

Query: 301 FGNYYFNVMIFPSPEMFNDDENCYGLVKQLREET-NKINGEMAKKFLNEDEDLMRTVKEI 360
            GN    +      E   D  +  G ++   E+T +  N E+ K         M  + E+
Sbjct: 301 MGNIATLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDDHNHELLKG--------MTCLYEL 360

Query: 361 ASKVVNGEIISCAFSSICRFPLYDVDFGWGRPV---WVTFPALWFKNLVAFLDSKDDDAI 420
             +    E++S  F+S CR   YD+DFGWG+P+     TFP    +N    +D++  D +
Sbjct: 361 EPQ----ELLS--FTSWCRLGFYDLDFGWGKPLSACTTTFPK---RNAALLMDTRSGDGV 414

Query: 421 DAIVHLEEQYMNKLEADELFL 430
           +A + + E  M  L  + L L
Sbjct: 421 EAWLPMAEDEMAMLPVELLSL 414

BLAST of Lsi08G016180 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.8e-47
Identity = 142/457 (31.07%), Postives = 229/457 (50.11%), Query Frame = 0

Query: 3   LEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFALA 62
           +E + +S+ LI+PS+PTP  L  Y LS+ DQ     Y  A +FY + DG           
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFYSNPDGHE------IST 66

Query: 63  FKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPDDL 122
            + +L+++L   L  Y P AG+    + +I+CND G+ F + R++ ++ D++   +P+  
Sbjct: 67  IREQLQNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVDVRIHCRMNDIL---KPE-- 126

Query: 123 NRLFPVEMDRFNEELMA-------VQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAA 182
            R +  E+ R N   +        VQ + F CGGVAVA  ISHK+ADA T+ S   +WAA
Sbjct: 127 LRSYASELIRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWAA 186

Query: 183 MARGVK---DVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAE 242
               +    DV  P +    IFP +            N V +RF+F P  +E +++K  E
Sbjct: 187 STCDLSSSHDVSTPVLVSDSIFPRQDNIICGQFPASPNCVRKRFLFSPEAIERLKSKAIE 246

Query: 243 NEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPN---DMSYLINYTVNLRPKMNPPLP 302
                   +P+R+E L+AF+      A K  +   N    + + +   VNLRP +   LP
Sbjct: 247 ----FGIEKPTRVEVLTAFLCRCATVAGKSAAKNNNCGQSLPFAVIQAVNLRPLLE--LP 306

Query: 303 QDAFGNYYFNVMIFPSPEMFNDDENCYG-----LVKQLREETNKINGEMAKK--FLNEDE 362
           +++ GN    + I+ S    ND  N        ++ +LR+  +K+     +K  ++   +
Sbjct: 307 KNSVGNL---ISIYFSTIKENDTVNIEQEFTKLVIGELRKAKDKLKNLSQEKLNYVARMQ 366

Query: 363 DLMRTVKEIASK----VVNGEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVA 422
           D    +KE+       + N +I +  FSS CRFP YD+DFG G+P+WV      FKN + 
Sbjct: 367 DFANCLKELDISSFFDMENVDIDAYLFSSWCRFPFYDIDFGLGKPIWVCMFQPHFKNCII 426

Query: 423 FLDSK--DDDAIDAIVHLEEQYMNKLEADELFLEYAT 434
            +D    DD  I+A++ LE++ M   E +EL L +A+
Sbjct: 427 LMDYPFGDDYGIEALITLEQEKMPAFENNELLLSFAS 442

BLAST of Lsi08G016180 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 8.4e-44
Identity = 130/433 (30.02%), Postives = 217/433 (50.12%), Query Frame = 0

Query: 5   VDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFALAFK 64
           V+I+S+  I+PS+PTP  LRRY LS IDQ T+     +L F+ + +    E        K
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQ-TIDSEVTSLAFFYTYN-PSHESSKIGDLLK 65

Query: 65  NRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPDDLNR 124
           N L  TL   +S+Y   AGR    + +I+CND GV F E R++ ++ D+++  +    + 
Sbjct: 66  NSLSKTL---VSYY-QFAGR-LIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDL 125

Query: 125 LFPVEMDRFNEE-LMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARGVKDV 184
           + P  +   +E+ L+ VQ + F CGG+A+    SHK++D ++      +WA+ +  +   
Sbjct: 126 VLPTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWAS-STSLSTF 185

Query: 185 FKPH--VEGAKIFPPK--TMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 244
            KP   +    IFPP+   +  N S+   +  + +RFVF    +E +++K  E       
Sbjct: 186 HKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIE----YGI 245

Query: 245 TRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYFN 304
            +PSR+E ++A     FL        +P    Y I   VNLRP +   LPQ++ GN +  
Sbjct: 246 QKPSRVEVVTA-----FLCQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIFSF 305

Query: 305 VMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNGE 364
                   M   D     L+ +LR    K+   +  K     E  M+ + +   ++    
Sbjct: 306 YFCINDEGM---DNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLDQLNISS 365

Query: 365 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQYM 424
           + +   SS CRFP+YD+DFGWG+P+ V+      K+L+  +DS + D I+A++ +EE+ M
Sbjct: 366 LDTYFCSSWCRFPVYDIDFGWGKPILVSPFQPHVKDLILLMDSPEGDGIEALITMEEKKM 416

Query: 425 NKLEADELFLEYA 433
              E +E    +A
Sbjct: 426 AAFEKNEELRSFA 416

BLAST of Lsi08G016180 vs. ExPASy TrEMBL
Match: A0A5A7UYQ7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001680 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.0e-230
Identity = 391/444 (88.06%), Postives = 419/444 (94.37%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLR Y LSFIDQITVHIYAPALYFY+S D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFI+CND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAM +FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VK VFKPH+EGAKIFPPK MSY+T+MTIVRNRV+RRFVF+ SKLEAIRAKY EN+  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 TRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYFN 300
            RPSRIESL+AFVYSRFLAA+KH+S+I NDMS+L+NYTVNLRPKMNPPLP+DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNGE 360
           VMIFPSPE  NDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM TVKE+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQYM 420
           IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKD + +DAIVHLEE+YM
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLEADELFLEYATPIPTLSSPLA 444
           NKLE DE+F+ YATP+PT SSPLA
Sbjct: 421 NKLEGDEVFMNYATPLPTPSSPLA 444

BLAST of Lsi08G016180 vs. ExPASy TrEMBL
Match: A0A0A0LQS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.1e-227
Identity = 387/445 (86.97%), Postives = 420/445 (94.38%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLR Y LSFIDQITVHIYAPALYFY+S D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFI+CND GVPFREA VNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAMT+FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VK VFKPH+EGAKIFPPK MSY+++MTIVRNRV+RRFVF+ SK+EAIR KY EN+ MINQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 241 T-RPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYF 300
             +PSRIESL+AFVYSRFLAA+KH+S I NDMS+L+NYTVNLRPKMNPPLP DAFGNYYF
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKHDSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300

Query: 301 NVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNG 360
           NVMIFPSPE  NDDENCYGLVKQLREETNKI+GEMAKKFLNED++LM+TVKE+ASKVV+G
Sbjct: 301 NVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVVSG 360

Query: 361 EIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQY 420
           EI+SCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKD + IDAIVHLEE+Y
Sbjct: 361 EIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEERY 420

Query: 421 MNKLEADELFLEYATPIPTLSSPLA 444
           MNKLE DE+F++YATPIPT SS +A
Sbjct: 421 MNKLEGDEVFMKYATPIPTPSSRVA 445

BLAST of Lsi08G016180 vs. ExPASy TrEMBL
Match: A0A1S3BD01 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.2e-222
Identity = 377/424 (88.92%), Postives = 402/424 (94.81%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLR Y LSFIDQITVHIYAPALYFY+S D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFI+CND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAM +FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VK VFKPH+EGAKIFPPK MSY+T+MTIVRNRV+RRFVF+ SKLEAIRAKY EN+  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 TRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYFN 300
            RPSRIESL+AFVYSRFLAA+KH+S+I NDMS+L+NYTVNLRPKMNPPLP+DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNGE 360
           VMIFPSPE  NDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM TVKE+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQYM 420
           IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKD + +DAIVHLEE+YM
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLE 424
           NKLE
Sbjct: 421 NKLE 424

BLAST of Lsi08G016180 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.6e-191
Identity = 333/439 (75.85%), Postives = 380/439 (86.56%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           MKLE+DIISE+LI+P+TPTPPHLRRYPLSFIDQITVHIYAPALYFY+    +   D DFA
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQP---NRAGDPDFA 60

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPD 120
           + F+NRLRSTL  VLSHYPPLAGRPNY S+F++CND G  FREARVN++LADVV++A+PD
Sbjct: 61  IDFRNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPD 120

Query: 121 DLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARGV 180
           DLNRLFPVE+D+FNEELMAVQFTEFACGGVAVASCISHKIADAMT+FSLNNNWAAMARGV
Sbjct: 121 DLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGV 180

Query: 181 KDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQT 240
           K VFKPH+EGAK+FP KTM+Y+T+ TI+RNRVARRFVFE SK+EAIR KY EN A     
Sbjct: 181 KGVFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AH 240

Query: 241 RPSRIESLSAFVYSRFLAAYKHESS--IPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYF 300
           RPSR+ESL+AF+Y RFLAA K  SS  +P+D + L+NYTVNLR KM PPLP DAFGNYYF
Sbjct: 241 RPSRVESLTAFMYGRFLAALKDGSSSQVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYF 300

Query: 301 NVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNG 360
           N MIFPSP++ N D  CYGLV +LREETNK+N +  K  LNED+ LM +V+EIASK V G
Sbjct: 301 NAMIFPSPDILN-DPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKG 360

Query: 361 EIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQY 420
           E+ISCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSK+D  IDAIVHLEE +
Sbjct: 361 EMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPH 420

Query: 421 MNKLEADELFLEYATPIPT 438
           M KLEAD+LFL+YATP PT
Sbjct: 421 MKKLEADQLFLQYATPTPT 432

BLAST of Lsi08G016180 vs. ExPASy TrEMBL
Match: A0A6J1KPC8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.6e-189
Identity = 331/439 (75.40%), Postives = 375/439 (85.42%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           MKLE+DIISE+LI+P+TPTPPHLR YPLSFIDQITVHIYAPALYFYR        D DFA
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAG---DPDFA 60

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPD 120
           + F+NRLRSTL  VLSHYPPLAGRPNY S+F++CND G  FREARVN++LADVV++AQPD
Sbjct: 61  IHFRNRLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPD 120

Query: 121 DLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARGV 180
           DLNRLFPVE+D+FNEELMAVQFTEFACGGVAVASCISHKIADAMT+FSLNNNWAAMARGV
Sbjct: 121 DLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGV 180

Query: 181 KDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQT 240
           K VFKPH+EGAK+FP KT  Y+T+ TI+RNRVARRFVFE SK+EAIR KY EN A     
Sbjct: 181 KGVFKPHMEGAKLFPAKTTRYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AH 240

Query: 241 RPSRIESLSAFVYSRFLAAYKHESS--IPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYF 300
           RPSR+ESL+AF+Y RFLAA K  SS  +P+D + L+NYTVNLRPKM PPLP DAFGNYYF
Sbjct: 241 RPSRVESLTAFLYGRFLAALKEGSSSEVPSDRACLVNYTVNLRPKMEPPLPGDAFGNYYF 300

Query: 301 NVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNG 360
           N MIFPSP++ N D  C+GLV +LREETNK+N +  K  LNED+ LM +V+EIASK V G
Sbjct: 301 NAMIFPSPDILN-DPYCHGLVNRLREETNKLNAQTVKYILNEDQRLMESVEEIASKFVKG 360

Query: 361 EIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQY 420
           E+ISCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV  LDSK+   IDAIVHLEE +
Sbjct: 361 EMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTLLDSKEAGGIDAIVHLEEPH 420

Query: 421 MNKLEADELFLEYATPIPT 438
           M +LEAD+LFLEYATP PT
Sbjct: 421 MKRLEADQLFLEYATPTPT 432

BLAST of Lsi08G016180 vs. NCBI nr
Match: XP_038886173.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 813.1 bits (2099), Expect = 1.1e-231
Identity = 399/444 (89.86%), Postives = 418/444 (94.14%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLRRYPLS IDQITVHIYAP LYFYRS D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRRYPLSLIDQITVHIYAPTLYFYRSTDSGSGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYP LAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPQLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAMT+FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VKDVFKPH+EGAKIFP KTMSY+T+MT+VRNRVARRFVFE SKLEAIRAKYAEN+A INQ
Sbjct: 181 VKDVFKPHMEGAKIFPAKTMSYDTAMTLVRNRVARRFVFEQSKLEAIRAKYAENQATINQ 240

Query: 241 TRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYFN 300
            RPSRIESLSAFVYSRF+AA KH S++ ND+S+LINYTVNLRPKMNPPLP DAFGNYYFN
Sbjct: 241 IRPSRIESLSAFVYSRFIAALKHGSAMSNDVSFLINYTVNLRPKMNPPLPHDAFGNYYFN 300

Query: 301 VMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNGE 360
           VMIFPSPEM N DENCYGLVKQLREETNKINGEMAKKFLNE++DLM TVK+ ASKVVNGE
Sbjct: 301 VMIFPSPEMLNTDENCYGLVKQLREETNKINGEMAKKFLNENKDLMETVKDFASKVVNGE 360

Query: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQYM 420
           IISCAFSS CR PLYDVDFGWGRPVWVTFP LWFKNLVAFL+SKD D IDAIVHLEEQYM
Sbjct: 361 IISCAFSSTCRLPLYDVDFGWGRPVWVTFPGLWFKNLVAFLESKDGDGIDAIVHLEEQYM 420

Query: 421 NKLEADELFLEYATPIPTLSSPLA 444
           NKLEADE+FL+YA  IPT SSPLA
Sbjct: 421 NKLEADEVFLKYAATIPTPSSPLA 444

BLAST of Lsi08G016180 vs. NCBI nr
Match: KAA0061032.1 (vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 808.9 bits (2088), Expect = 2.2e-230
Identity = 391/444 (88.06%), Postives = 419/444 (94.37%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLR Y LSFIDQITVHIYAPALYFY+S D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFI+CND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAM +FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VK VFKPH+EGAKIFPPK MSY+T+MTIVRNRV+RRFVF+ SKLEAIRAKY EN+  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 TRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYFN 300
            RPSRIESL+AFVYSRFLAA+KH+S+I NDMS+L+NYTVNLRPKMNPPLP+DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNGE 360
           VMIFPSPE  NDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM TVKE+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQYM 420
           IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKD + +DAIVHLEE+YM
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLEADELFLEYATPIPTLSSPLA 444
           NKLE DE+F+ YATP+PT SSPLA
Sbjct: 421 NKLEGDEVFMNYATPLPTPSSPLA 444

BLAST of Lsi08G016180 vs. NCBI nr
Match: XP_004143048.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical protein Csa_018752 [Cucumis sativus])

HSP 1 Score: 797.3 bits (2058), Expect = 6.5e-227
Identity = 387/445 (86.97%), Postives = 420/445 (94.38%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLR Y LSFIDQITVHIYAPALYFY+S D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFI+CND GVPFREA VNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAMT+FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VK VFKPH+EGAKIFPPK MSY+++MTIVRNRV+RRFVF+ SK+EAIR KY EN+ MINQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKPMSYDSAMTIVRNRVSRRFVFKQSKVEAIRTKYTENQTMINQ 240

Query: 241 T-RPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYF 300
             +PSRIESL+AFVYSRFLAA+KH+S I NDMS+L+NYTVNLRPKMNPPLP DAFGNYYF
Sbjct: 241 NHQPSRIESLTAFVYSRFLAAFKHDSEIRNDMSFLVNYTVNLRPKMNPPLPHDAFGNYYF 300

Query: 301 NVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNG 360
           NVMIFPSPE  NDDENCYGLVKQLREETNKI+GEMAKKFLNED++LM+TVKE+ASKVV+G
Sbjct: 301 NVMIFPSPETLNDDENCYGLVKQLREETNKIDGEMAKKFLNEDKELMKTVKEVASKVVSG 360

Query: 361 EIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQY 420
           EI+SCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKD + IDAIVHLEE+Y
Sbjct: 361 EIVSCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGIDAIVHLEERY 420

Query: 421 MNKLEADELFLEYATPIPTLSSPLA 444
           MNKLE DE+F++YATPIPT SS +A
Sbjct: 421 MNKLEGDEVFMKYATPIPTPSSRVA 445

BLAST of Lsi08G016180 vs. NCBI nr
Match: XP_008445555.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 377/424 (88.92%), Postives = 402/424 (94.81%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSAD-GDGGEDLDF 60
           MKLEVDIISEELI+PSTPTPPHLR Y LSFIDQITVHIYAPALYFY+S D G GGEDLDF
Sbjct: 1   MKLEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDF 60

Query: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQP 120
           ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFI+CND GVPFREARVNSQLADV+QFAQP
Sbjct: 61  ALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQP 120

Query: 121 DDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 180
           DDLNRLFPVE+DRFNEELMAVQFTEFACGGVAVASCISHKIADAM +FSLNNNWAAMARG
Sbjct: 121 DDLNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAMARG 180

Query: 181 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQ 240
           VK VFKPH+EGAKIFPPK MSY+T+MTIVRNRV+RRFVF+ SKLEAIRAKY EN+  INQ
Sbjct: 181 VKGVFKPHMEGAKIFPPKMMSYDTAMTIVRNRVSRRFVFKQSKLEAIRAKYTENQTTINQ 240

Query: 241 TRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYFN 300
            RPSRIESL+AFVYSRFLAA+KH+S+I NDMS+L+NYTVNLRPKMNPPLP+DAFGNYYFN
Sbjct: 241 NRPSRIESLTAFVYSRFLAAFKHDSAIRNDMSFLVNYTVNLRPKMNPPLPRDAFGNYYFN 300

Query: 301 VMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNGE 360
           VMIFPSPE  NDDENCY LVKQLREETNKI+GEMAKKFLNED+DLM TVKE+ASKV NGE
Sbjct: 301 VMIFPSPETLNDDENCYDLVKQLREETNKIDGEMAKKFLNEDKDLMETVKEVASKVANGE 360

Query: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQYM 420
           IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKD + +DAIVHLEE+YM
Sbjct: 361 IISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDGEGVDAIVHLEERYM 420

Query: 421 NKLE 424
           NKLE
Sbjct: 421 NKLE 424

BLAST of Lsi08G016180 vs. NCBI nr
Match: KAG7020113.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678.7 bits (1750), Expect = 3.4e-191
Identity = 333/439 (75.85%), Postives = 381/439 (86.79%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           MKLE+DIISE+LI+P+TPTPPHLRRYPLSFIDQITVHIYAPALYFY+    +   D DFA
Sbjct: 1   MKLEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQP---NRAGDPDFA 60

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPD 120
           + F+NRLRSTL  VLSHYPPLAGRPNY S+F++CND G  FREARVN++LADVV++A+PD
Sbjct: 61  IDFRNRLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPD 120

Query: 121 DLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARGV 180
           DLNRLFPVE+D+FNEELMAVQFTEFACGGVAVASCISHKIADAMT+FSLNNNWAAMARGV
Sbjct: 121 DLNRLFPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAMARGV 180

Query: 181 KDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAENEAMINQT 240
           K VFKPH+EGAK+FP KTM+Y+T+ TI+RNRVARRFVFE SK+EAIR KY EN A     
Sbjct: 181 KGVFKPHMEGAKLFPAKTMTYDTAKTILRNRVARRFVFEGSKVEAIREKYGENGA---AH 240

Query: 241 RPSRIESLSAFVYSRFLAAYKHESS--IPNDMSYLINYTVNLRPKMNPPLPQDAFGNYYF 300
           RPSR+ESL+AF+Y RFLAA K  SS  +P+D + L+NYTVNLR KM PPLP DAFGNYYF
Sbjct: 241 RPSRVESLTAFMYGRFLAALKDGSSSEVPSDRTCLVNYTVNLRSKMEPPLPGDAFGNYYF 300

Query: 301 NVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIASKVVNG 360
           N MIFPSP++ N D  CYGLV +LREETNK+N +  K  LNED+ LM +V+EIASK V G
Sbjct: 301 NAMIFPSPDILN-DPYCYGLVDRLREETNKLNAQTVKYILNEDQSLMESVEEIASKFVKG 360

Query: 361 EIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIVHLEEQY 420
           E+ISCAF+SICRFPLYDVDFGWGRPVWVTFPALWFKNLV FLDSK+D  IDAIVHLEE +
Sbjct: 361 EMISCAFTSICRFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKEDGGIDAIVHLEEPH 420

Query: 421 MNKLEADELFLEYATPIPT 438
           MNKL+AD+LFL+YATP PT
Sbjct: 421 MNKLQADQLFLQYATPTPT 432

BLAST of Lsi08G016180 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 292.0 bits (746), Expect = 8.2e-79
Identity = 160/446 (35.87%), Postives = 264/446 (59.19%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYP----LSFIDQITVHIYAPALYFYRSADGDGGED 60
           M LE+ + S+EL++PS   P +L   P    LSF+DQ+   I+ P L+FY +      ++
Sbjct: 1   MGLEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKE 60

Query: 61  LDFALAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQF 120
                   + ++S+L ++L+ Y PLAGR   +   + CND GV F EA+ +  ++ +++ 
Sbjct: 61  R------SDHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILEN 120

Query: 121 AQPDDLNRLFPVEMDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAM 180
             P++LN+L P E    ++  + VQ T F CGG+A+   +SHK+ DA++     N+WAA 
Sbjct: 121 PNPNELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAF 180

Query: 181 ARGVKD-VFKPHVEGAKIFPP---KTMSYNTSMTIVRNRVARRFVFEPSKLEAIRAKYAE 240
           ARG  D +  P  + AK+FPP   + ++  T +T   N V RRFVF  S +E++R +++ 
Sbjct: 181 ARGQTDEIITPSFDLAKMFPPCDIENLNMATGIT-KENIVTRRFVFLRSSVESLRERFSG 240

Query: 241 NEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQDA 300
           N+    + R +R+E LS F++SRF+A+  H+        Y + + VNLR + +P +P + 
Sbjct: 241 NK----KIRATRVEVLSVFIWSRFMASTNHDDK--TGKIYTLIHPVNLRRQADPDIPDNM 300

Query: 301 FGNYYFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKEIA 360
           FGN     +  P   +  +DE    LV Q+REE  KI+    KK   ++   +  + + A
Sbjct: 301 FGNIMRFSVTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQA 360

Query: 361 SKVVNGEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDAIDAIV 420
           S  VNGEI+S +F+S+C+FP+Y+ DFGWG+P+WV    + +KNLVAF+D+K+ D I+A +
Sbjct: 361 SGFVNGEIVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWI 420

Query: 421 HLEEQYMNKLEADELFLEYATPIPTL 439
           +L++  M++ EADE  L Y +  P++
Sbjct: 421 NLDQNDMSRFEADEELLRYVSSNPSV 430

BLAST of Lsi08G016180 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.6e-64
Identity = 152/455 (33.41%), Postives = 252/455 (55.38%), Query Frame = 0

Query: 3   LEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADG-DGGEDLDFAL 62
           + VD++S ++I+PS+PTP HL+++ LS ++Q+   I+ P ++FY + +     E L    
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQM-- 60

Query: 63  AFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPDD 122
                L+ +L + L+H+ PLAGR    +  I+CND+G  F EARVNS L++++     D 
Sbjct: 61  -----LKKSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDS 120

Query: 123 LNRLFPVEMDRF--NEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAM-AR 182
           L +L P  +D       L+  Q + F CG +++  CISHK+ADA ++     +WAA+ +R
Sbjct: 121 LQQLIPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSR 180

Query: 183 G-VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRV------ARRFVFEPSKLEAIRAKYA 242
           G +K +  P  +  KIFPP   S  +   +V   +      ++RF+F+ S ++A++AK +
Sbjct: 181 GSIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKAS 240

Query: 243 ENEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQD 302
             E  +NQ  P+R+E++SA ++   + A +  S   +  S L N +V+LR +++PP  ++
Sbjct: 241 SFE--VNQ--PTRVEAVSALIWKSAMKATRTVSG-TSKPSILAN-SVSLRSRVSPPFTKN 300

Query: 303 AFGNY--YFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFL---NEDEDLMR 362
           + GN   YF        E   +      LV ++R+   +       K +   N  E +  
Sbjct: 301 SIGNLVSYFAA----KAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICS 360

Query: 363 TVKEIASKVVNGEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDD 422
             KE    + +G+     FSS CRF LY+ DFGWG+PVWV FP++  KN+V  LD+K+  
Sbjct: 361 YQKEAGDMIASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAG 420

Query: 423 AIDAIVHLEEQYMNKLEADELFLEYATPIPTLSSP 442
            I+A V+L EQ MN  E D   L++A+  P++  P
Sbjct: 421 GIEAWVNLNEQEMNLFEQDRELLQFASLNPSVIQP 437

BLAST of Lsi08G016180 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-50
Identity = 147/468 (31.41%), Postives = 236/468 (50.43%), Query Frame = 0

Query: 3   LEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFALA 62
           ++V+ I++E+I+PS+ TP  L+   LS  D I   +Y  A  FY        +DL     
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTK------DDLISPEQ 63

Query: 63  FKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNS-QLADVVQFAQPDD 122
             ++L+++L + L+ + PLAGR    +  I+CND G  F +ARVN+  L+D ++      
Sbjct: 64  SSHKLKTSLAETLTKFYPLAGRIKGVT--IDCNDEGAVFVDARVNNYPLSDFLRSPDFKT 123

Query: 123 LNRLFPVEM--DRFNE----ELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAA 182
           L +L P+++  D +       L+ V+ T F CGG+A+  CI+HKIADA ++ +   +WAA
Sbjct: 124 LQQLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 183

Query: 183 MARGVKD--VFKPHVEGAKIFPPKTMSYNTSMTIVRNRVA---RRFVFEPSKLEAIRAKY 242
           MARG     V  P    A  +PP    +   +    N+++   +RFVF  SKLE +R K 
Sbjct: 184 MARGEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKA 243

Query: 243 AENEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPPLPQ 302
           A  + +    RP R+ES++A ++  F+AA    ++   DM  LI  + NLRPK+   L +
Sbjct: 244 ASEDFV---GRPKRVESVTALLWKAFVAAASSNTN-ACDMKVLIQPS-NLRPKIPSLLAE 303

Query: 303 DAFGNYYFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTVKE 362
              GN  F+ ++              G+ +Q   +  K   ++ K    + +DL   +KE
Sbjct: 304 SLIGNIMFSSVVL-------------GISQQEEIKIEKAVRDLRK----QGDDLQNVIKE 363

Query: 363 --------IASKVVNGEIISCA-----------FSSICRFPLYDVDFGWGRPVWVT-FPA 422
                   I SK+ N  + + +            SS C+ PLY+  FGWG PVW+    A
Sbjct: 364 EGGSSSAMIGSKLANLMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVWIAGNVA 423

Query: 423 LWFKNLVAFLDSKDDDAIDAIVHLEEQYMNKLEADELFLEYATPIPTL 439
              +N+   +DSKD   I+A V L E+ M  LE +   L +A+  P++
Sbjct: 424 PMLENVTMLIDSKDGQGIEAFVTLPEENMLSLEQNTELLAFASVNPSV 441

BLAST of Lsi08G016180 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 196.1 bits (497), Expect = 6.2e-50
Identity = 142/460 (30.87%), Postives = 228/460 (49.57%), Query Frame = 0

Query: 1   MKLEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFA 60
           M ++V+ IS+E+I+PS+PTP +L+   LS  D I   +Y  A  FY   D    E     
Sbjct: 4   MTMKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHT--- 63

Query: 61  LAFKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNS-QLADVVQFAQP 120
               ++L+++L + L+ + PLAGR       ++C D G  F +ARVN+  L + ++    
Sbjct: 64  ---SHKLKTSLSETLTKFYPLAGR--ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDF 123

Query: 121 DDLNRLFPVE------MDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNW 180
           D L +L P++      +      L+ V+ T F CGG+A+  CI+HKIADA ++ +   +W
Sbjct: 124 DALQQLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSW 183

Query: 181 AAMARGVKD---VFKPHVEGAKIFPPKTMSYN---TSMTIVRNRVARRFVFEPSKLEAIR 240
           AA ARG  D   +  P   GA  +PP   ++          R+ + +RFVFE SK+E +R
Sbjct: 184 AATARGENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLR 243

Query: 241 AKYAENEAMINQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPP 300
            K A  E +    +P+R+ES++A ++  F+A+ K  +    D   L+    NLR K+   
Sbjct: 244 TKAASEETV---DQPTRVESVTALIWKCFVASSKTTTC---DHKVLVQ-LANLRSKIPSL 303

Query: 301 LPQDAFGNYYFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRT 360
           L + + GN  F+ ++         +      V+ LR++  ++   +  +  + D   M  
Sbjct: 304 LQESSIGNLMFSSVVLSIGR--GGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIG 363

Query: 361 VKEIASKVVNGEIIS------CAFSSICRFPLYDVDFGWGRPVWV---TFPALWFKNLVA 420
            K     + N   +S         SS C+ PLY+  FGW  PVWV     P L   NL  
Sbjct: 364 SKLANLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVL--GNLAM 423

Query: 421 FLDSKDDDAIDAIVHLEEQYMNKLEADELFLEYATPIPTL 439
            +DSKD   I+A V L E+ M+  E +   L +AT  P++
Sbjct: 424 LIDSKDGQGIEAFVTLPEENMSSFEQNPELLAFATMNPSV 444

BLAST of Lsi08G016180 vs. TAIR 10
Match: AT5G23970.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-47
Identity = 140/451 (31.04%), Postives = 227/451 (50.33%), Query Frame = 0

Query: 3   LEVDIISEELIRPSTPTPPHLRRYPLSFIDQITVHIYAPALYFYRSADGDGGEDLDFALA 62
           ++++++S+E+I+P++P   HL+   LS  DQ     Y  A++FY   D    ED+     
Sbjct: 1   MKLELLSKEVIKPASPN--HLQTLSLSLFDQFLPSTYVSAIFFY--DDHSNQEDI----- 60

Query: 63  FKNRLRSTLPDVLSHYPPLAGRPNYASSFINCNDAGVPFREARVNSQLADVVQFAQPDDL 122
              RL+S+L   LS + PLAG+     + ++CND G  F EAR    L+D ++     DL
Sbjct: 61  --QRLKSSLSQTLSLFYPLAGQIKDGVT-VHCNDEGALFTEARAEIFLSDFLRNPSDADL 120

Query: 123 NRLFPVEMDRFNEE---LMAVQFTEFACGGVAVASCISHKIADAMTVFSLNNNWAAMARG 182
            + F V  D  + E   L+ V+   F   G AVA  +SHKI DA ++ +   +W   ++G
Sbjct: 121 IQKFIVSPDHADPETWPLLHVKVIFFKDKGFAVAVSVSHKICDAASLSTFVCSWTKASKG 180

Query: 183 VKDVFKPHVEGAKIFPPKTMSYNTSMTIVRNRVA--RRFVFEPSKLEAIRAKYAENEAMI 242
             D   P   GA  +PP  +S      +V    +  +RFVF    +E ++ + +  + + 
Sbjct: 181 YADTVDPEFVGADFYPPADISIEFPPLLVHETKSKTKRFVFGSLMIEKLKNRASSGKRVP 240

Query: 243 NQTRPSRIESLSAFVYSRFLAAYKHESSIPNDMSYLINYTVNLRPKMNPP-LPQDAFGNY 302
             T   RIES++A +  R +    H  S      + I  T++LRP+++   LP  A GN+
Sbjct: 241 QAT---RIESITALLL-RCMTKAGHSKS-GKVKEFAITQTMDLRPRVSSSLLPHKAIGNF 300

Query: 303 YFNVMIFPSPEMFNDDENCYGLVKQLREETNKINGEMAKKFLNEDEDLMRTV---KEIAS 362
           +F  ++  S E   + E     +++ ++E N++         N+ ED   +V   + IAS
Sbjct: 301 FFLPLLKESSESKMEIEETVSKLQKTKQELNEL-------IRNDSEDAKSSVEAKERIAS 360

Query: 363 KV------VNGEIISCAFSSICRFPLYDVDFGWGRPVWVTFPALWFKNLVAFLDSKDDDA 422
            +      ++ E+ + A SS CR   Y+ +FGWG+PVWV  P    K  V  +DSKD + 
Sbjct: 361 AMLSSLCEISPEMETYAVSSWCRMSFYEANFGWGKPVWVA-PDSVDKTQVVLMDSKDSEG 420

Query: 423 IDAIVHLEEQYMNKLEADELFLEYATPIPTL 439
           ++A V L E  M K E D   L YATP P++
Sbjct: 421 VEARVTLPETDMAKYEHDSELLVYATPSPSI 426