Homology
BLAST of Lsi08G015850 vs. ExPASy Swiss-Prot
Match:
Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)
HSP 1 Score: 413.3 bits (1061), Expect = 4.1e-114
Identity = 239/585 (40.85%), Postives = 329/585 (56.24%), Query Frame = 0
Query: 6 NYKKFPKSFATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISH 65
+++ + + T Y+F L GPM YKGIYHLFYQ+NP GAV++ ++VW HS S
Sbjct: 30 SHQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSV 89
Query: 66 DLINWVHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSS 125
DL+NW+ A P+ P DINGCWSGSV+ L KPVILYTGID + QVQN+A+P N S
Sbjct: 90 DLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNIS 149
Query: 126 DPFLEKWRKSSQNPIIAT--PDGVERDKFRDPTR--LGRAQMEDGE------SPLEGRRA 185
DP+L +W K QNP++ T +G+ D+FRDPT LGR DGE S + RR
Sbjct: 150 DPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGR----DGEWRVIVGSSTDDRRG 209
Query: 186 M----EEQRCFTEVRISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMK 245
+ + + F + PL+ + WECPDF+PV + G+ G++ SS G+K+V+K
Sbjct: 210 LAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLK 269
Query: 246 ASF--NSHDYYTLGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMIS 305
S HDYYT+GSY EK+ ++ D G N R Y + +
Sbjct: 270 VSLIETLHDYYTIGSYDREKDVYVPDLGF-----VQNESAPRLDYGKYYASKTFYDDVKK 329
Query: 306 RRDGLDCSMNWMNWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWPV---------- 365
RR W+N + + E +Q+ PRKIWL ++G++L+QWP+
Sbjct: 330 RR----ILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQ 389
Query: 366 ------------------------DVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDAS 425
DVEVSF+ LE+A+ ++ W DPQ +CS+ D S
Sbjct: 390 VNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWT--DPQKICSQGDLS 449
Query: 426 VNGRVGPFGLLVLASNDLSEHTAIFFHILKAHD------RYVVLMCSDQSRLHPCRGFQF 485
V +GPFGL+VLASND+ E+T+++F I K++D +YVVLMCSDQSR
Sbjct: 450 VMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSR--------- 509
Query: 486 TSILKVFFGLQTQLNWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKG 524
SS + DK+T+GAFV +DP H+ ISLRTL+DHSIVES+GG G
Sbjct: 510 -----------------SSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGG 569
BLAST of Lsi08G015850 vs. ExPASy Swiss-Prot
Match:
Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)
HSP 1 Score: 391.7 bits (1005), Expect = 1.3e-107
Identity = 234/556 (42.09%), Postives = 307/556 (55.22%), Query Frame = 0
Query: 26 GPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPNDPFDINGCWSG 85
GPM YKGIYHLFYQ NP GAV+ + +VWAHS S DLINW A+ P+ PFDINGCWSG
Sbjct: 69 GPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSG 128
Query: 86 SVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNPIIATPD---GVE 145
S + L KPVILYTGID QVQN+A P N SDP+L +W+KS NP++A PD G+
Sbjct: 129 SATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMA-PDAVNGIN 188
Query: 146 RDKFRDPTRLGRAQMEDGESPLEGRRAMEEQRCFTEVRISF--------PLYSSRDSWTW 205
FRDPT Q + + G + T F PL+ S W
Sbjct: 189 ASSFRDPTTAWLGQ-DKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMW 248
Query: 206 ECPDFYPVMLNGTIGIDFSSGFRVG--VKYVMKASFN--SHDYYTLGSYAPEKEKFISDS 265
ECPDF+PV G+ G++ SS +K+V+K S + HDYYT+G+Y K+KF+ D+
Sbjct: 249 ECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDN 308
Query: 266 GHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWFLNFE---- 325
G + D GT G Y ++ W N + S + E
Sbjct: 309 GFKMD--GTAPRYDYGKY-------YASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWS 368
Query: 326 -MQAVPRKIWLSKAGRQLIQWPV-----------------------------------DV 385
+Q +PRKIWL ++G+QLIQWPV DV
Sbjct: 369 GIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADV 428
Query: 386 EVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFF 445
EV F+ LE+A+ ++ W DPQL+CSK + SV +GPFGL+VLAS +L E+T+++F
Sbjct: 429 EVLFKVRDLEKADVIEPSWT--DPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYF 488
Query: 446 HILKAH---DRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVDKTTY 505
I KA ++YVVLMCSDQSR SS + DKTTY
Sbjct: 489 RIFKARQNSNKYVVLMCSDQSR--------------------------SSLKEDNDKTTY 548
Query: 506 GAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNNGTQT 524
GAFVD++P H+ +SLR L+DHS+VESFGGKG+ CITSRVYP LA+ + L+AFN G Q+
Sbjct: 549 GAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQS 583
BLAST of Lsi08G015850 vs. ExPASy Swiss-Prot
Match:
Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)
HSP 1 Score: 385.2 bits (988), Expect = 1.2e-105
Identity = 228/571 (39.93%), Postives = 320/571 (56.04%), Query Frame = 0
Query: 14 FATNYNFPHLLKGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEP 73
F + N+ + GPM YKGIYHLFYQ+ P V+++++VW HS S DLINW AL P
Sbjct: 61 FQSPKNWMNDPNGPMIYKGIYHLFYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSP 120
Query: 74 NDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNP 133
++P+DINGCWSGS++ L +NKPVILYTGI++ +QVQNLA+P N SDP+L++W K QNP
Sbjct: 121 SEPYDINGCWSGSITILPQNKPVILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNP 180
Query: 134 IIA-TP---DGVERDKFRDPTRLGRAQMEDGE-----SPLEGRRAMEEQRCFTE------ 193
++A TP + + FRDP+ Q+ DG+ +G+R + FT
Sbjct: 181 LMAGTPTNNNNINASSFRDPSTAW--QLSDGKWRVIVGTQQGKRGLAV--LFTSDDFVKW 240
Query: 194 VRISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASF--NSHDYYTL 253
PL+S+ + WECPDF+PV + ++G D +S VK+V+K S ++YYT+
Sbjct: 241 NNTGNPLHSTEGNGIWECPDFFPVYVGKSLGAD-TSIIGDDVKHVLKLSLFDTQYEYYTI 300
Query: 254 GSYAPEKEKFI-------SDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLD 313
G Y EK+ ++ SD G R+D+ Y + + I +
Sbjct: 301 GRYDIEKDIYVPDEGSIESDLGLRYDYGKF--------YASKSFFDDETNRRILWGWVNE 360
Query: 314 CSMNWMNWNESWFLNFEMQAVPRKIWLSKAGRQLIQWPV--------------------- 373
S+ + + W +QA+PR + L K+G+QL+QWP+
Sbjct: 361 SSIQADDIKKGW---SGVQAIPRTVVLDKSGKQLVQWPLAEVDMLRENDVELPSQVIKGG 420
Query: 374 -------------DVEVSFEFP---YLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPF 433
DVE+SF+ P Y+EE +S T +PQ+LCS++ AS+ GR GPF
Sbjct: 421 SLVEISQITASQADVEISFKIPESNYVEELDSTCT-----NPQILCSQKGASIKGRFGPF 480
Query: 434 GLLVLASNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQL 493
GLL LAS L E+TA+FF I K ++YVVLMCSDQ+R
Sbjct: 481 GLLTLASMGLEEYTAVFFRIFKGPNKYVVLMCSDQTR----------------------- 540
Query: 494 NWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV 524
SS DK ++G FVDVDP +E +SLR L+DHSIVESF KGK+CIT+RVYPT+A+
Sbjct: 541 ---SSLNPTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARVYPTMAI 584
BLAST of Lsi08G015850 vs. ExPASy Swiss-Prot
Match:
Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)
HSP 1 Score: 374.4 bits (960), Expect = 2.1e-102
Identity = 218/543 (40.15%), Postives = 299/543 (55.06%), Query Frame = 0
Query: 14 FATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHL 73
+ T Y+F L GPM Y G YHLFYQ+NP GAV+ + +VWAHS+S DL+NW L
Sbjct: 46 YRTAYHFQPLKNWINDPNGPMRYGGFYHLFYQYNPKGAVWGN-IVWAHSVSKDLVNWTPL 105
Query: 74 NHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWR 133
+HA+ P+ P DI GCWSGS + L KP ILYTGID + HQVQN+AIP N SDP L +W+
Sbjct: 106 DHAIHPSQPSDIKGCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWK 165
Query: 134 KSSQNPII--ATPDGVERDKFRDPTR--LGR----AQMEDGESPLEGRRAMEEQRCFTE- 193
KS +NP++ + + FRDPT LG+ + + +G + + + F +
Sbjct: 166 KSPKNPLMEPTIANKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDW 225
Query: 194 VRISFPLYSSRDSWTWECPDFYPVMLNGTI--GIDFSSGFRVGVKYVMKASFN--SHDYY 253
V PL+S+ + WECPDFYPV+ + G+D S V++V+K S + HD+Y
Sbjct: 226 VEAKHPLHSAEGTGMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHY 285
Query: 254 TLGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNW 313
+GSY K+ F+ ++G F+ G L G Y ++ + W
Sbjct: 286 LIGSYDVVKDVFVPENG--FEDNGFVLRYDYGKY-------YASKTFFDDGKNRRILLGW 345
Query: 314 MNWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWPV--------------------- 373
+N + S + + + +PR+IWL ++G+QL+QWPV
Sbjct: 346 VNESSSVADDVKKGWSGIHTIPREIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGG 405
Query: 374 -------------DVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLL 433
DVE+SFE L + ES+ +W +DPQLLCS++ A V G VGPFGLL
Sbjct: 406 ERISITGVDSVQADVEISFEISDLGKVESL-RKW--IDPQLLCSQKGAGVKGGVGPFGLL 465
Query: 434 VLASNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWL 493
V AS L E+TA+FF I K D+ +VLMCSDQSR
Sbjct: 466 VFASQGLKEYTAVFFRIFKYQDKNLVLMCSDQSR-------------------------- 525
Query: 494 SSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDND 499
SS D T+YG FVDVDP HEK+SLRTL+DHS+VESFGG+G+ C+T+RVYPTLA+ +
Sbjct: 526 SSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVTARVYPTLAIHDK 549
BLAST of Lsi08G015850 vs. ExPASy Swiss-Prot
Match:
Q0J360 (Beta-fructofuranosidase, insoluble isoenzyme 7 OS=Oryza sativa subsp. japonica OX=39947 GN=CIN7 PE=2 SV=1)
HSP 1 Score: 367.9 bits (943), Expect = 2.0e-100
Identity = 217/552 (39.31%), Postives = 296/552 (53.62%), Query Frame = 0
Query: 26 GPMFYKGIYHLFYQHNPNGAVFN-SKMVWAHSISHDLINWVHLNHALEPNDPFDINGCWS 85
GPM++ G+YHLFYQ+NP+ A+++ + W HS+S DL+NW L+ AL+P PFD NGCWS
Sbjct: 57 GPMYHNGMYHLFYQYNPHSALWDIGNLSWGHSVSGDLLNWAALDTALDPTSPFDANGCWS 116
Query: 86 GSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNPIIATPDGVERD 145
GS + L P ILYTGID+S QVQN+A N SDP L +W K + NP+IA P V D
Sbjct: 117 GSATILPGALPAILYTGIDASKEQVQNVAFAKNPSDPLLREWEKPAYNPVIALPADVPGD 176
Query: 146 KFRDPT----------RLGRAQMEDGESPLEGRRAMEEQRCFTEVRISFPLYSSRDSWTW 205
KFRDP+ R+ + DG + R+ + R R + PL++SR +
Sbjct: 177 KFRDPSTAWLGRDGLWRIAVSAEVDGVASTLVYRSKDFVRW---ERNAAPLHASRAAGMV 236
Query: 206 ECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASF--NSHDYYTLGSYAPEKEKFISDSGH 265
ECPD +PV G G+D S+ GV++V+K S DYY +G+Y + F
Sbjct: 237 ECPDLFPVAERGEDGLDTSANGAGGVRHVLKLSVMDTLQDYYMVGTYDDAADAFSPAEPE 296
Query: 266 RFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWFLNFE-----M 325
R D + RR Y V + +RR W N ++S + +
Sbjct: 297 RGDDCRS---WRRLDYGHVYASKSFFDVRKNRR----VLWAWANESDSQADDVARGWSGV 356
Query: 326 QAVPRKIWLSKAGRQLIQWPV-----------------------------------DVEV 385
Q PRK+WL+K G+QL+QWP+ DVEV
Sbjct: 357 QTFPRKMWLAKDGKQLLQWPIEEIKTLRRKRAGLWQGTRLGAGAVQEIVGVASSQADVEV 416
Query: 386 SFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFFHI 445
F+ P LEEAE VD LDPQ LC ++ A+V G VGPFGLLV+AS DL EHTA+FF +
Sbjct: 417 VFKIPSLEEAERVDDPNRLLDPQKLCGEKGAAVRGGVGPFGLLVMASGDLHEHTAVFFRV 476
Query: 446 LKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVDKTTYGAFVD 505
+ HD+Y +LMC+D ++ SS R GV K YG FVD
Sbjct: 477 FRHHDKYKLLMCTDLTK--------------------------SSTRAGVYKPAYGGFVD 536
Query: 506 VD-PRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNNGTQTVVIS 524
+D H+ ISLRTL+DHS+VESFGG G+ CIT+RVYP + + LY FNNG+ V ++
Sbjct: 537 MDIDDHKTISLRTLIDHSVVESFGGGGRACITARVYPEHVATSSSHLYVFNNGSDAVKVA 572
BLAST of Lsi08G015850 vs. ExPASy TrEMBL
Match:
A0A1S3BB78 (LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS=Cucumis melo OX=3656 GN=LOC103487780 PE=3 SV=1)
HSP 1 Score: 684.1 bits (1764), Expect = 4.6e-193
Identity = 364/561 (64.88%), Postives = 397/561 (70.77%), Query Frame = 0
Query: 16 TNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNH 75
T+Y+F L GPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNH
Sbjct: 51 TSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNH 110
Query: 76 ALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKS 135
ALEP DPFDINGCWSGSVSFL KNKP ILYTGIDSSF QVQNLAIPTN SDPFLEKWRKS
Sbjct: 111 ALEPTDPFDINGCWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKS 170
Query: 136 SQNPIIATPDGVERDKFRDPTRLGRAQMED------GESPLEGRRAMEEQRCFTE-VRIS 195
QNPIIA PDG ER++FRDPT + + G++ G + F +
Sbjct: 171 PQNPIIAPPDGAERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFR 230
Query: 196 FPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLGSYAPE 255
FPLYSS+DS TWECPDFYPVMLNGT GI FSSGF VGVK+VMKASFNS D+YTLGSY PE
Sbjct: 231 FPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPE 290
Query: 256 KEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWF 315
KEKF SD G FDFRG NL R Y + RR W+N ++S
Sbjct: 291 KEKFTSDHGLGFDFRGINL-GLRYDYGKFYASKTFYDSSQKRR----ILWGWVNESDSRQ 350
Query: 316 LNFE-----MQAVPRKIWLSKAGRQLIQWP------------------------------ 375
+ +QA+PRKIWLSK GRQLIQWP
Sbjct: 351 DDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGG 410
Query: 376 ----VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLS 435
VDVEVSF P+LEEAESVDTRW LDPQ+LCSKRDAS+NGRVGPFGLLVLASNDLS
Sbjct: 411 SASQVDVEVSFALPHLEEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLS 470
Query: 436 EHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVD 495
EHTAI+FH+LKAH+RYVVLMCSD+SR SSFR GVD
Sbjct: 471 EHTAIYFHVLKAHNRYVVLMCSDESR--------------------------SSFRKGVD 530
Query: 496 KTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNN 525
KTTYGAFVD+DPR++KISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV+NDARLYAFNN
Sbjct: 531 KTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNDARLYAFNN 580
BLAST of Lsi08G015850 vs. ExPASy TrEMBL
Match:
A0A6J1HFM6 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462607 PE=3 SV=1)
HSP 1 Score: 672.9 bits (1735), Expect = 1.1e-189
Identity = 364/571 (63.75%), Postives = 395/571 (69.18%), Query Frame = 0
Query: 4 EINYKKFPKSF-ATNYNFPHL---LKGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISH 63
+IN P+ F T+Y+F L + GPM+YKGIYHLFYQHNPNGAVF+SK+VW H++S
Sbjct: 34 DINEVHIPQQFQRTSYHFQPLQNWMNGPMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSR 93
Query: 64 DLINWVHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSS 123
DLINW HLNHALEP PFDINGCWSGSVSFL NKPVILYTG+DSSF QVQNLAIP+N S
Sbjct: 94 DLINWAHLNHALEPTHPFDINGCWSGSVSFLHNNKPVILYTGLDSSFQQVQNLAIPSNFS 153
Query: 124 DPFLEKWRKSSQNPIIATPDGVERDKFRDPTRL------GRAQMEDGESPLEGRRAMEEQ 183
DPFLE W KSS NPIIA P+G+ER +FRDPT G + G++ G +
Sbjct: 154 DPFLEIWTKSSHNPIIAPPNGIERHRFRDPTTAWPGPDGGWRIVIGGQTSYGGAAMLYRS 213
Query: 184 RCFTEVRIS-FPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVG-VKYVMKASFNSH 243
F S FPLYSSRDS WECPDFYPV LNG+ GID +GFRVG VKYVMKASFNSH
Sbjct: 214 EDFVHWHQSRFPLYSSRDSGNWECPDFYPVKLNGSNGIDSLNGFRVGAVKYVMKASFNSH 273
Query: 244 DYYTLGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCS 303
DYYTLGSY PEKEKF SDSG FD RGTNL R Y + RR
Sbjct: 274 DYYTLGSYVPEKEKFTSDSGDGFDLRGTNL-GLRYDYGKFYASKTFYDSSQKRR----IL 333
Query: 304 MNWMNWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWP------------------- 363
W+N ++S N + +Q VPRKIWLSK GRQLIQWP
Sbjct: 334 WGWVNESDSREDNIKKGWAGLQVVPRKIWLSKTGRQLIQWPVKEVEKLRTNRFSLYNKEL 393
Query: 364 ---------------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPF 423
VD EVSFEFPYLEEAE VDTRW DLDPQLLCSKRDASVNGRVGPF
Sbjct: 394 RGRSTMKVLGGSASQVDAEVSFEFPYLEEAERVDTRWFDLDPQLLCSKRDASVNGRVGPF 453
Query: 424 GLLVLASNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQL 483
GLLVLASNDLSEHTAIFF +LKAH+RYVVLMCSDQSR
Sbjct: 454 GLLVLASNDLSEHTAIFFRVLKAHNRYVVLMCSDQSR----------------------- 513
Query: 484 NWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV 524
SSFR GVDKTTYGAFVDVDPRHEKISLRTLVD SIVESFGGKGKTCITSRVYPTLAV
Sbjct: 514 ---SSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVYPTLAV 573
BLAST of Lsi08G015850 vs. ExPASy TrEMBL
Match:
A0A2I4JRG1 (Cell wall invertase 4 OS=Cucumis sativus OX=3659 PE=2 SV=1)
HSP 1 Score: 672.5 bits (1734), Expect = 1.4e-189
Identity = 361/566 (63.78%), Postives = 395/566 (69.79%), Query Frame = 0
Query: 11 PKSFATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINW 70
P+ T+Y+F L GPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINW
Sbjct: 41 PQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINW 100
Query: 71 VHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLE 130
VHLNHALEP DPFDINGCWSGSVSFL +NKP ILYTGIDSS QVQNLAIP N SDPFLE
Sbjct: 101 VHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLE 160
Query: 131 KWRKSSQNPIIATPDGVERDKFRDPTRLGRAQMED------GESPLEGRRAMEEQRCFTE 190
KWRK SQNPIIA PDG+ER++FRDPT + + G++ G + F
Sbjct: 161 KWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVR 220
Query: 191 -VRISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLG 250
+ FPLYSS+DS TWECPDFYPVMLNGT GI FSSGF VGVKYVMKASFNS D+YTLG
Sbjct: 221 WHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLG 280
Query: 251 SYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNW 310
SY PEKEKF SD G FDF+G NL R Y + RR W+N
Sbjct: 281 SYVPEKEKFTSDYGPGFDFKGINL-GLRYDYGKFYASKTFYDSSQKRR----ILWGWVNE 340
Query: 311 NESWFLNFE-----MQAVPRKIWLSKAGRQLIQWP------------------------- 370
++S + +QA+PRKIWLSK GRQLIQWP
Sbjct: 341 SDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTM 400
Query: 371 ---------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLA 430
VDVEVSFE PYLEEAESVDTRW LDPQ+LCSKRDASVNGRVGPFGLLVLA
Sbjct: 401 EVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLA 460
Query: 431 SNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSF 490
SNDLSEHTAI+FHILKAH+RYVVLMCSDQSR SSF
Sbjct: 461 SNDLSEHTAIYFHILKAHNRYVVLMCSDQSR--------------------------SSF 520
Query: 491 RNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARL 525
R VDKT +GAFVD+DP ++KISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV+N+ARL
Sbjct: 521 RKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARL 575
BLAST of Lsi08G015850 vs. ExPASy TrEMBL
Match:
A0A0A0LQX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351670 PE=3 SV=1)
HSP 1 Score: 672.5 bits (1734), Expect = 1.4e-189
Identity = 361/566 (63.78%), Postives = 395/566 (69.79%), Query Frame = 0
Query: 11 PKSFATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINW 70
P+ T+Y+F L GPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINW
Sbjct: 62 PQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINW 121
Query: 71 VHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLE 130
VHLNHALEP DPFDINGCWSGSVSFL +NKP ILYTGIDSS QVQNLAIP N SDPFLE
Sbjct: 122 VHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLE 181
Query: 131 KWRKSSQNPIIATPDGVERDKFRDPTRLGRAQMED------GESPLEGRRAMEEQRCFTE 190
KWRK SQNPIIA PDG+ER++FRDPT + + G++ G + F
Sbjct: 182 KWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVR 241
Query: 191 -VRISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLG 250
+ FPLYSS+DS TWECPDFYPVMLNGT GI FSSGF VGVKYVMKASFNS D+YTLG
Sbjct: 242 WHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLG 301
Query: 251 SYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNW 310
SY PEKEKF SD G FDF+G NL R Y + RR W+N
Sbjct: 302 SYVPEKEKFTSDYGPGFDFKGINL-GLRYDYGKFYASKTFYDSSQKRR----ILWGWVNE 361
Query: 311 NESWFLNFE-----MQAVPRKIWLSKAGRQLIQWP------------------------- 370
++S + +QA+PRKIWLSK GRQLIQWP
Sbjct: 362 SDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTM 421
Query: 371 ---------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLA 430
VDVEVSFE PYLEEAESVDTRW LDPQ+LCSKRDASVNGRVGPFGLLVLA
Sbjct: 422 EVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLA 481
Query: 431 SNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSF 490
SNDLSEHTAI+FHILKAH+RYVVLMCSDQSR SSF
Sbjct: 482 SNDLSEHTAIYFHILKAHNRYVVLMCSDQSR--------------------------SSF 541
Query: 491 RNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARL 525
R VDKT +GAFVD+DP ++KISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV+N+ARL
Sbjct: 542 RKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARL 596
BLAST of Lsi08G015850 vs. ExPASy TrEMBL
Match:
A0A6J1HDH1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462607 PE=3 SV=1)
HSP 1 Score: 670.2 bits (1728), Expect = 6.9e-189
Identity = 364/574 (63.41%), Postives = 394/574 (68.64%), Query Frame = 0
Query: 4 EINYKKFPKSF-ATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHS 63
+IN P+ F T+Y+F L GPM+YKGIYHLFYQHNPNGAVF+SK+VW H+
Sbjct: 34 DINEVHIPQQFQRTSYHFQPLQNWMNDPNGPMYYKGIYHLFYQHNPNGAVFDSKIVWGHA 93
Query: 64 ISHDLINWVHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPT 123
+S DLINW HLNHALEP PFDINGCWSGSVSFL NKPVILYTG+DSSF QVQNLAIP+
Sbjct: 94 VSRDLINWAHLNHALEPTHPFDINGCWSGSVSFLHNNKPVILYTGLDSSFQQVQNLAIPS 153
Query: 124 NSSDPFLEKWRKSSQNPIIATPDGVERDKFRDPTRL------GRAQMEDGESPLEGRRAM 183
N SDPFLE W KSS NPIIA P+G+ER +FRDPT G + G++ G +
Sbjct: 154 NFSDPFLEIWTKSSHNPIIAPPNGIERHRFRDPTTAWPGPDGGWRIVIGGQTSYGGAAML 213
Query: 184 EEQRCFTEVRIS-FPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVG-VKYVMKASF 243
F S FPLYSSRDS WECPDFYPV LNG+ GID +GFRVG VKYVMKASF
Sbjct: 214 YRSEDFVHWHQSRFPLYSSRDSGNWECPDFYPVKLNGSNGIDSLNGFRVGAVKYVMKASF 273
Query: 244 NSHDYYTLGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGL 303
NSHDYYTLGSY PEKEKF SDSG FD RGTNL R Y + RR
Sbjct: 274 NSHDYYTLGSYVPEKEKFTSDSGDGFDLRGTNL-GLRYDYGKFYASKTFYDSSQKRR--- 333
Query: 304 DCSMNWMNWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWP---------------- 363
W+N ++S N + +Q VPRKIWLSK GRQLIQWP
Sbjct: 334 -ILWGWVNESDSREDNIKKGWAGLQVVPRKIWLSKTGRQLIQWPVKEVEKLRTNRFSLYN 393
Query: 364 ------------------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRV 423
VD EVSFEFPYLEEAE VDTRW DLDPQLLCSKRDASVNGRV
Sbjct: 394 KELRGRSTMKVLGGSASQVDAEVSFEFPYLEEAERVDTRWFDLDPQLLCSKRDASVNGRV 453
Query: 424 GPFGLLVLASNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQ 483
GPFGLLVLASNDLSEHTAIFF +LKAH+RYVVLMCSDQSR
Sbjct: 454 GPFGLLVLASNDLSEHTAIFFRVLKAHNRYVVLMCSDQSR-------------------- 513
Query: 484 TQLNWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPT 524
SSFR GVDKTTYGAFVDVDPRHEKISLRTLVD SIVESFGGKGKTCITSRVYPT
Sbjct: 514 ------SSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVYPT 573
BLAST of Lsi08G015850 vs. NCBI nr
Match:
XP_038884233.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Benincasa hispida])
HSP 1 Score: 700.7 bits (1807), Expect = 9.8e-198
Identity = 373/543 (68.69%), Postives = 394/543 (72.56%), Query Frame = 0
Query: 28 MFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPNDPFDINGCWSGSV 87
MFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEP+DPFDINGCWSGSV
Sbjct: 1 MFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPSDPFDINGCWSGSV 60
Query: 88 SFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNPIIATPDGVERDKFR 147
SFLLKNKP+ILYTGIDSSF QVQNLAIP N SDPFLEKWRKSSQNPIIA P+GVERDKFR
Sbjct: 61 SFLLKNKPLILYTGIDSSFQQVQNLAIPINFSDPFLEKWRKSSQNPIIAPPNGVERDKFR 120
Query: 148 DPTRLGRAQMED------GESPLEGRRAMEEQRCFTE-VRISFPLYSSRDSWTWECPDFY 207
DPT + + G + G + F + FPLYSSRDS TWECPDFY
Sbjct: 121 DPTTAWQGPDGEWRVAVGGHTSYGGAAMLYRSEDFVHWHQFRFPLYSSRDSGTWECPDFY 180
Query: 208 PVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLGSYAPEKEKFISDSGHRFDFRGTN 267
PVMLNGT GIDFSSGF GVKYVMKASFNSHDYYTLGSYA EKEKF SDS FDFRGTN
Sbjct: 181 PVMLNGTNGIDFSSGFGFGVKYVMKASFNSHDYYTLGSYATEKEKFTSDSSSGFDFRGTN 240
Query: 268 LVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWFLNFE-----MQAVPRKIW 327
L R Y + RR W+N ++S + + +QAVPRKIW
Sbjct: 241 L-GLRYDYGKFYASKTFYDSSKKRR----ILWGWVNESDSREDDIKKGWSGLQAVPRKIW 300
Query: 328 LSKAGRQLIQWP----------------------------------VDVEVSFEFPYLEE 387
LSK GRQL QWP VDVEVSFE P LEE
Sbjct: 301 LSKTGRQLTQWPVKEIEMLRRNHFSLHHKELRGRSTMEILGGSASQVDVEVSFESPNLEE 360
Query: 388 AESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFFHILKAHDRYVV 447
AESV+TRW DLDPQLLCSKRDASVNGR+GPFGLLVLASNDLSEHTAIFFHILKAH+RYVV
Sbjct: 361 AESVETRWDDLDPQLLCSKRDASVNGRIGPFGLLVLASNDLSEHTAIFFHILKAHNRYVV 420
Query: 448 LMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVDKTTYGAFVDVDPRHEKIS 507
LMCSDQSR SSFR GVDKTTYGAFVDVDP H+KIS
Sbjct: 421 LMCSDQSR--------------------------SSFRKGVDKTTYGAFVDVDPCHKKIS 480
Query: 508 LRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNNGTQTVVISSLKVWNMSNA 525
LRTLVDH+IVESFGGKGKTCITSRVYPTLAV NDARLYAFNNGTQTVVISSL+VWNMSNA
Sbjct: 481 LRTLVDHTIVESFGGKGKTCITSRVYPTLAVANDARLYAFNNGTQTVVISSLEVWNMSNA 512
BLAST of Lsi08G015850 vs. NCBI nr
Match:
XP_008444460.1 (PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Cucumis melo])
HSP 1 Score: 684.1 bits (1764), Expect = 9.5e-193
Identity = 364/561 (64.88%), Postives = 397/561 (70.77%), Query Frame = 0
Query: 16 TNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNH 75
T+Y+F L GPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHLNH
Sbjct: 51 TSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHLNH 110
Query: 76 ALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKS 135
ALEP DPFDINGCWSGSVSFL KNKP ILYTGIDSSF QVQNLAIPTN SDPFLEKWRKS
Sbjct: 111 ALEPTDPFDINGCWSGSVSFLPKNKPRILYTGIDSSFQQVQNLAIPTNYSDPFLEKWRKS 170
Query: 136 SQNPIIATPDGVERDKFRDPTRLGRAQMED------GESPLEGRRAMEEQRCFTE-VRIS 195
QNPIIA PDG ER++FRDPT + + G++ G + F +
Sbjct: 171 PQNPIIAPPDGAERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQFR 230
Query: 196 FPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLGSYAPE 255
FPLYSS+DS TWECPDFYPVMLNGT GI FSSGF VGVK+VMKASFNS D+YTLGSY PE
Sbjct: 231 FPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKHVMKASFNSRDHYTLGSYVPE 290
Query: 256 KEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWF 315
KEKF SD G FDFRG NL R Y + RR W+N ++S
Sbjct: 291 KEKFTSDHGLGFDFRGINL-GLRYDYGKFYASKTFYDSSQKRR----ILWGWVNESDSRQ 350
Query: 316 LNFE-----MQAVPRKIWLSKAGRQLIQWP------------------------------ 375
+ +QA+PRKIWLSK GRQLIQWP
Sbjct: 351 DDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVLGG 410
Query: 376 ----VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLS 435
VDVEVSF P+LEEAESVDTRW LDPQ+LCSKRDAS+NGRVGPFGLLVLASNDLS
Sbjct: 411 SASQVDVEVSFALPHLEEAESVDTRWGVLDPQVLCSKRDASMNGRVGPFGLLVLASNDLS 470
Query: 436 EHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVD 495
EHTAI+FH+LKAH+RYVVLMCSD+SR SSFR GVD
Sbjct: 471 EHTAIYFHVLKAHNRYVVLMCSDESR--------------------------SSFRKGVD 530
Query: 496 KTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNN 525
KTTYGAFVD+DPR++KISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV+NDARLYAFNN
Sbjct: 531 KTTYGAFVDIDPRYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNDARLYAFNN 580
BLAST of Lsi08G015850 vs. NCBI nr
Match:
XP_011649529.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus])
HSP 1 Score: 675.2 bits (1741), Expect = 4.4e-190
Identity = 361/563 (64.12%), Postives = 396/563 (70.34%), Query Frame = 0
Query: 11 PKSFATNYNFPHL---LKGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHL 70
P+ T+Y+F L + GPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINWVHL
Sbjct: 73 PQVQRTSYHFQPLKNWMNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINWVHL 132
Query: 71 NHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWR 130
NHALEP DPFDINGCWSGSVSFL +NKP ILYTGIDSS QVQNLAIP N SDPFLEKWR
Sbjct: 133 NHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLEKWR 192
Query: 131 KSSQNPIIATPDGVERDKFRDPTRLGRAQMED------GESPLEGRRAMEEQRCFTE-VR 190
K SQNPIIA PDG+ER++FRDPT + + G++ G + F +
Sbjct: 193 KFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVRWHQ 252
Query: 191 ISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLGSYA 250
FPLYSS+DS TWECPDFYPVMLNGT GI FSSGF VGVKYVMKASFNS D+YTLGSY
Sbjct: 253 FRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLGSYV 312
Query: 251 PEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNES 310
PEKEKF SD G FDF+G NL R Y + RR W+N ++S
Sbjct: 313 PEKEKFTSDYGPGFDFKGINL-GLRYDYGKFYASKTFYDSSQKRR----ILWGWVNESDS 372
Query: 311 WFLNFE-----MQAVPRKIWLSKAGRQLIQWP---------------------------- 370
+ +QA+PRKIWLSK GRQLIQWP
Sbjct: 373 RQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTMEVL 432
Query: 371 ------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASND 430
VDVEVSFE PYLEEAESVDTRW LDPQ+LCSKRDASVNGRVGPFGLLVLASND
Sbjct: 433 GGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLASND 492
Query: 431 LSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNG 490
LSEHTAI+FHILKAH+RYVVLMCSDQSR SSFR
Sbjct: 493 LSEHTAIYFHILKAHNRYVVLMCSDQSR--------------------------SSFRKE 552
Query: 491 VDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAF 525
VDKT +GAFVD+DP ++KISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV+N+ARLYAF
Sbjct: 553 VDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARLYAF 604
BLAST of Lsi08G015850 vs. NCBI nr
Match:
XP_022962009.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 [Cucurbita moschata])
HSP 1 Score: 672.9 bits (1735), Expect = 2.2e-189
Identity = 364/571 (63.75%), Postives = 395/571 (69.18%), Query Frame = 0
Query: 4 EINYKKFPKSF-ATNYNFPHL---LKGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISH 63
+IN P+ F T+Y+F L + GPM+YKGIYHLFYQHNPNGAVF+SK+VW H++S
Sbjct: 34 DINEVHIPQQFQRTSYHFQPLQNWMNGPMYYKGIYHLFYQHNPNGAVFDSKIVWGHAVSR 93
Query: 64 DLINWVHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSS 123
DLINW HLNHALEP PFDINGCWSGSVSFL NKPVILYTG+DSSF QVQNLAIP+N S
Sbjct: 94 DLINWAHLNHALEPTHPFDINGCWSGSVSFLHNNKPVILYTGLDSSFQQVQNLAIPSNFS 153
Query: 124 DPFLEKWRKSSQNPIIATPDGVERDKFRDPTRL------GRAQMEDGESPLEGRRAMEEQ 183
DPFLE W KSS NPIIA P+G+ER +FRDPT G + G++ G +
Sbjct: 154 DPFLEIWTKSSHNPIIAPPNGIERHRFRDPTTAWPGPDGGWRIVIGGQTSYGGAAMLYRS 213
Query: 184 RCFTEVRIS-FPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVG-VKYVMKASFNSH 243
F S FPLYSSRDS WECPDFYPV LNG+ GID +GFRVG VKYVMKASFNSH
Sbjct: 214 EDFVHWHQSRFPLYSSRDSGNWECPDFYPVKLNGSNGIDSLNGFRVGAVKYVMKASFNSH 273
Query: 244 DYYTLGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCS 303
DYYTLGSY PEKEKF SDSG FD RGTNL R Y + RR
Sbjct: 274 DYYTLGSYVPEKEKFTSDSGDGFDLRGTNL-GLRYDYGKFYASKTFYDSSQKRR----IL 333
Query: 304 MNWMNWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWP------------------- 363
W+N ++S N + +Q VPRKIWLSK GRQLIQWP
Sbjct: 334 WGWVNESDSREDNIKKGWAGLQVVPRKIWLSKTGRQLIQWPVKEVEKLRTNRFSLYNKEL 393
Query: 364 ---------------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPF 423
VD EVSFEFPYLEEAE VDTRW DLDPQLLCSKRDASVNGRVGPF
Sbjct: 394 RGRSTMKVLGGSASQVDAEVSFEFPYLEEAERVDTRWFDLDPQLLCSKRDASVNGRVGPF 453
Query: 424 GLLVLASNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQL 483
GLLVLASNDLSEHTAIFF +LKAH+RYVVLMCSDQSR
Sbjct: 454 GLLVLASNDLSEHTAIFFRVLKAHNRYVVLMCSDQSR----------------------- 513
Query: 484 NWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV 524
SSFR GVDKTTYGAFVDVDPRHEKISLRTLVD SIVESFGGKGKTCITSRVYPTLAV
Sbjct: 514 ---SSFREGVDKTTYGAFVDVDPRHEKISLRTLVDRSIVESFGGKGKTCITSRVYPTLAV 573
BLAST of Lsi08G015850 vs. NCBI nr
Match:
XP_004142928.3 (beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus] >KAE8652051.1 hypothetical protein Csa_018697 [Cucumis sativus])
HSP 1 Score: 672.5 bits (1734), Expect = 2.9e-189
Identity = 361/566 (63.78%), Postives = 395/566 (69.79%), Query Frame = 0
Query: 11 PKSFATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINW 70
P+ T+Y+F L GPMFYKGIYH FYQHNPNGAVFNSKMVWAHSIS+DLINW
Sbjct: 73 PQVQRTSYHFQPLKNWMNDPNGPMFYKGIYHFFYQHNPNGAVFNSKMVWAHSISYDLINW 132
Query: 71 VHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLE 130
VHLNHALEP DPFDINGCWSGSVSFL +NKP ILYTGIDSS QVQNLAIP N SDPFLE
Sbjct: 133 VHLNHALEPTDPFDINGCWSGSVSFLPENKPRILYTGIDSSSQQVQNLAIPANYSDPFLE 192
Query: 131 KWRKSSQNPIIATPDGVERDKFRDPTRLGRAQMED------GESPLEGRRAMEEQRCFTE 190
KWRK SQNPIIA PDG+ER++FRDPT + + G++ G + F
Sbjct: 193 KWRKFSQNPIIAPPDGLERNRFRDPTTAWQGPDGEWRVAIGGQTSYGGAAMLYRSEDFVR 252
Query: 191 -VRISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASFNSHDYYTLG 250
+ FPLYSS+DS TWECPDFYPVMLNGT GI FSSGF VGVKYVMKASFNS D+YTLG
Sbjct: 253 WHQFRFPLYSSQDSGTWECPDFYPVMLNGTNGIGFSSGFGVGVKYVMKASFNSSDHYTLG 312
Query: 251 SYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNW 310
SY PEKEKF SD G FDF+G NL R Y + RR W+N
Sbjct: 313 SYVPEKEKFTSDYGPGFDFKGINL-GLRYDYGKFYASKTFYDSSQKRR----ILWGWVNE 372
Query: 311 NESWFLNFE-----MQAVPRKIWLSKAGRQLIQWP------------------------- 370
++S + +QA+PRKIWLSK GRQLIQWP
Sbjct: 373 SDSRQDDINKGWSGLQAIPRKIWLSKTGRQLIQWPVKEIKMLRRNHFSLHHKELRGRSTM 432
Query: 371 ---------VDVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLA 430
VDVEVSFE PYLEEAESVDTRW LDPQ+LCSKRDASVNGRVGPFGLLVLA
Sbjct: 433 EVLGGSASQVDVEVSFELPYLEEAESVDTRWGVLDPQVLCSKRDASVNGRVGPFGLLVLA 492
Query: 431 SNDLSEHTAIFFHILKAHDRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSF 490
SNDLSEHTAI+FHILKAH+RYVVLMCSDQSR SSF
Sbjct: 493 SNDLSEHTAIYFHILKAHNRYVVLMCSDQSR--------------------------SSF 552
Query: 491 RNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARL 525
R VDKT +GAFVD+DP ++KISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV+N+ARL
Sbjct: 553 RKEVDKTAHGAFVDIDPCYKKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVNNNARL 607
BLAST of Lsi08G015850 vs. TAIR 10
Match:
AT1G55120.1 (beta-fructofuranosidase 5 )
HSP 1 Score: 413.3 bits (1061), Expect = 2.9e-115
Identity = 239/585 (40.85%), Postives = 329/585 (56.24%), Query Frame = 0
Query: 6 NYKKFPKSFATNYNFPHLL------KGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISH 65
+++ + + T Y+F L GPM YKGIYHLFYQ+NP GAV++ ++VW HS S
Sbjct: 30 SHQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSV 89
Query: 66 DLINWVHLNHALEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSS 125
DL+NW+ A P+ P DINGCWSGSV+ L KPVILYTGID + QVQN+A+P N S
Sbjct: 90 DLVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNIS 149
Query: 126 DPFLEKWRKSSQNPIIAT--PDGVERDKFRDPTR--LGRAQMEDGE------SPLEGRRA 185
DP+L +W K QNP++ T +G+ D+FRDPT LGR DGE S + RR
Sbjct: 150 DPYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGR----DGEWRVIVGSSTDDRRG 209
Query: 186 M----EEQRCFTEVRISFPLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMK 245
+ + + F + PL+ + WECPDF+PV + G+ G++ SS G+K+V+K
Sbjct: 210 LAILYKSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLK 269
Query: 246 ASF--NSHDYYTLGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMIS 305
S HDYYT+GSY EK+ ++ D G N R Y + +
Sbjct: 270 VSLIETLHDYYTIGSYDREKDVYVPDLGF-----VQNESAPRLDYGKYYASKTFYDDVKK 329
Query: 306 RRDGLDCSMNWMNWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWPV---------- 365
RR W+N + + E +Q+ PRKIWL ++G++L+QWP+
Sbjct: 330 RR----ILWGWVNESSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQ 389
Query: 366 ------------------------DVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDAS 425
DVEVSF+ LE+A+ ++ W DPQ +CS+ D S
Sbjct: 390 VNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVKELEKADVIEPSWT--DPQKICSQGDLS 449
Query: 426 VNGRVGPFGLLVLASNDLSEHTAIFFHILKAHD------RYVVLMCSDQSRLHPCRGFQF 485
V +GPFGL+VLASND+ E+T+++F I K++D +YVVLMCSDQSR
Sbjct: 450 VMSGLGPFGLMVLASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSR--------- 509
Query: 486 TSILKVFFGLQTQLNWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKG 524
SS + DK+T+GAFV +DP H+ ISLRTL+DHSIVES+GG G
Sbjct: 510 -----------------SSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGG 569
BLAST of Lsi08G015850 vs. TAIR 10
Match:
AT1G55120.3 (beta-fructofuranosidase 5 )
HSP 1 Score: 412.9 bits (1060), Expect = 3.8e-115
Identity = 235/561 (41.89%), Postives = 320/561 (57.04%), Query Frame = 0
Query: 24 LKGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPNDPFDINGCW 83
+ GPM YKGIYHLFYQ+NP GAV++ ++VW HS S DL+NW+ A P+ P DINGCW
Sbjct: 39 VSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCW 98
Query: 84 SGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNPIIAT--PDGV 143
SGSV+ L KPVILYTGID + QVQN+A+P N SDP+L +W K QNP++ T +G+
Sbjct: 99 SGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGI 158
Query: 144 ERDKFRDPTR--LGRAQMEDGE------SPLEGRRAM----EEQRCFTEVRISFPLYSSR 203
D+FRDPT LGR DGE S + RR + + + F + PL+
Sbjct: 159 NPDRFRDPTTAWLGR----DGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 218
Query: 204 DSWTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASF--NSHDYYTLGSYAPEKEKFI 263
+ WECPDF+PV + G+ G++ SS G+K+V+K S HDYYT+GSY EK+ ++
Sbjct: 219 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 278
Query: 264 SDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWFLNFE- 323
D G N R Y + + RR W+N + + E
Sbjct: 279 PDLGF-----VQNESAPRLDYGKYYASKTFYDDVKKRR----ILWGWVNESSPAKDDIEK 338
Query: 324 ----MQAVPRKIWLSKAGRQLIQWPV---------------------------------- 383
+Q+ PRKIWL ++G++L+QWP+
Sbjct: 339 GWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQA 398
Query: 384 DVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAI 443
DVEVSF+ LE+A+ ++ W DPQ +CS+ D SV +GPFGL+VLASND+ E+T++
Sbjct: 399 DVEVSFKVKELEKADVIEPSWT--DPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSV 458
Query: 444 FFHILKAHD------RYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGV 503
+F I K++D +YVVLMCSDQSR SS +
Sbjct: 459 YFRIFKSNDDTNKKTKYVVLMCSDQSR--------------------------SSLNDEN 518
Query: 504 DKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFN 524
DK+T+GAFV +DP H+ ISLRTL+DHSIVES+GG G+TCITSRVYP LA+ +A L+ FN
Sbjct: 519 DKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFN 558
BLAST of Lsi08G015850 vs. TAIR 10
Match:
AT1G55120.2 (beta-fructofuranosidase 5 )
HSP 1 Score: 412.5 bits (1059), Expect = 5.0e-115
Identity = 235/559 (42.04%), Postives = 319/559 (57.07%), Query Frame = 0
Query: 26 GPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPNDPFDINGCWSG 85
GPM YKGIYHLFYQ+NP GAV++ ++VW HS S DL+NW+ A P+ P DINGCWSG
Sbjct: 17 GPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDINGCWSG 76
Query: 86 SVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNPIIAT--PDGVER 145
SV+ L KPVILYTGID + QVQN+A+P N SDP+L +W K QNP++ T +G+
Sbjct: 77 SVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNAVNGINP 136
Query: 146 DKFRDPTR--LGRAQMEDGE------SPLEGRRAM----EEQRCFTEVRISFPLYSSRDS 205
D+FRDPT LGR DGE S + RR + + + F + PL+ +
Sbjct: 137 DRFRDPTTAWLGR----DGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYEDLT 196
Query: 206 WTWECPDFYPVMLNGTIGIDFSSGFRVGVKYVMKASF--NSHDYYTLGSYAPEKEKFISD 265
WECPDF+PV + G+ G++ SS G+K+V+K S HDYYT+GSY EK+ ++ D
Sbjct: 197 GMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYVPD 256
Query: 266 SGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWFLNFE--- 325
G N R Y + + RR W+N + + E
Sbjct: 257 LGF-----VQNESAPRLDYGKYYASKTFYDDVKKRR----ILWGWVNESSPAKDDIEKGW 316
Query: 326 --MQAVPRKIWLSKAGRQLIQWPV----------------------------------DV 385
+Q+ PRKIWL ++G++L+QWP+ DV
Sbjct: 317 SGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADV 376
Query: 386 EVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFF 445
EVSF+ LE+A+ ++ W DPQ +CS+ D SV +GPFGL+VLASND+ E+T+++F
Sbjct: 377 EVSFKVKELEKADVIEPSWT--DPQKICSQGDLSVMSGLGPFGLMVLASNDMEEYTSVYF 436
Query: 446 HILKAHD------RYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVDK 505
I K++D +YVVLMCSDQSR SS + DK
Sbjct: 437 RIFKSNDDTNKKTKYVVLMCSDQSR--------------------------SSLNDENDK 496
Query: 506 TTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNNG 524
+T+GAFV +DP H+ ISLRTL+DHSIVES+GG G+TCITSRVYP LA+ +A L+ FN G
Sbjct: 497 STFGAFVAIDPSHQTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKG 534
BLAST of Lsi08G015850 vs. TAIR 10
Match:
AT3G13790.2 (Glycosyl hydrolases family 32 protein )
HSP 1 Score: 392.5 bits (1007), Expect = 5.3e-109
Identity = 236/571 (41.33%), Postives = 314/571 (54.99%), Query Frame = 0
Query: 14 FATNYNF---PHLLKGPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHA 73
+ T ++F + + GPM YKGIYHLFYQ NP GAV+ + +VWAHS S DLINW A
Sbjct: 51 YRTGFHFQPPKNWMNGPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWDPHPPA 110
Query: 74 LEPNDPFDINGCWSGSVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSS 133
+ P+ PFDINGCWSGS + L KPVILYTGID QVQN+A P N SDP+L +W+KS
Sbjct: 111 IFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSP 170
Query: 134 QNPIIATPD---GVERDKFRDPTRLGRAQMEDGESPLEGRRAMEEQRCFTEVRISF---- 193
NP++A PD G+ FRDPT Q + + G + T F
Sbjct: 171 LNPLMA-PDAVNGINASSFRDPTTAWLGQ-DKKWRVIIGSKIHRRGLAITYTSKDFLKWE 230
Query: 194 ----PLYSSRDSWTWECPDFYPVMLNGTIGIDFSSGFRVG--VKYVMKASFN--SHDYYT 253
PL+ S WECPDF+PV G+ G++ SS +K+V+K S + HDYYT
Sbjct: 231 KSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYT 290
Query: 254 LGSYAPEKEKFISDSGHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWM 313
+G+Y K+KF+ D+G + D GT G Y ++ W
Sbjct: 291 IGTYDRVKDKFVPDNGFKMD--GTAPRYDYGKY-------YASKTFFDSAKNRRILWGWT 350
Query: 314 NWNESWFLNFE-----MQAVPRKIWLSKAGRQLIQWPV---------------------- 373
N + S + E +Q +PRKIWL ++G+QLIQWPV
Sbjct: 351 NESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSG 410
Query: 374 -------------DVEVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLL 433
DVEV F+ LE+A+ ++ W DPQL+CSK + SV +GPFGL+
Sbjct: 411 SRLEVYGVTAAQADVEVLFKVRDLEKADVIEPSWT--DPQLICSKMNVSVKSGLGPFGLM 470
Query: 434 VLASNDLSEHTAIFFHILKAH---DRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQL 493
VLAS +L E+T+++F I KA ++YVVLMCSDQSR
Sbjct: 471 VLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSDQSR----------------------- 530
Query: 494 NWLSSFRNGVDKTTYGAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAV 524
SS + DKTTYGAFVD++P H+ +SLR L+DHS+VESFGGKG+ CITSRVYP LA+
Sbjct: 531 ---SSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAI 580
BLAST of Lsi08G015850 vs. TAIR 10
Match:
AT3G13790.1 (Glycosyl hydrolases family 32 protein )
HSP 1 Score: 391.7 bits (1005), Expect = 9.0e-109
Identity = 234/556 (42.09%), Postives = 307/556 (55.22%), Query Frame = 0
Query: 26 GPMFYKGIYHLFYQHNPNGAVFNSKMVWAHSISHDLINWVHLNHALEPNDPFDINGCWSG 85
GPM YKGIYHLFYQ NP GAV+ + +VWAHS S DLINW A+ P+ PFDINGCWSG
Sbjct: 69 GPMIYKGIYHLFYQWNPKGAVWGN-IVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSG 128
Query: 86 SVSFLLKNKPVILYTGIDSSFHQVQNLAIPTNSSDPFLEKWRKSSQNPIIATPD---GVE 145
S + L KPVILYTGID QVQN+A P N SDP+L +W+KS NP++A PD G+
Sbjct: 129 SATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMA-PDAVNGIN 188
Query: 146 RDKFRDPTRLGRAQMEDGESPLEGRRAMEEQRCFTEVRISF--------PLYSSRDSWTW 205
FRDPT Q + + G + T F PL+ S W
Sbjct: 189 ASSFRDPTTAWLGQ-DKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMW 248
Query: 206 ECPDFYPVMLNGTIGIDFSSGFRVG--VKYVMKASFN--SHDYYTLGSYAPEKEKFISDS 265
ECPDF+PV G+ G++ SS +K+V+K S + HDYYT+G+Y K+KF+ D+
Sbjct: 249 ECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDN 308
Query: 266 GHRFDFRGTNLVQRRGGYCGVGLMNLIAEKMISRRDGLDCSMNWMNWNESWFLNFE---- 325
G + D GT G Y ++ W N + S + E
Sbjct: 309 GFKMD--GTAPRYDYGKY-------YASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWS 368
Query: 326 -MQAVPRKIWLSKAGRQLIQWPV-----------------------------------DV 385
+Q +PRKIWL ++G+QLIQWPV DV
Sbjct: 369 GIQTIPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADV 428
Query: 386 EVSFEFPYLEEAESVDTRWADLDPQLLCSKRDASVNGRVGPFGLLVLASNDLSEHTAIFF 445
EV F+ LE+A+ ++ W DPQL+CSK + SV +GPFGL+VLAS +L E+T+++F
Sbjct: 429 EVLFKVRDLEKADVIEPSWT--DPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYF 488
Query: 446 HILKAH---DRYVVLMCSDQSRLHPCRGFQFTSILKVFFGLQTQLNWLSSFRNGVDKTTY 505
I KA ++YVVLMCSDQSR SS + DKTTY
Sbjct: 489 RIFKARQNSNKYVVLMCSDQSR--------------------------SSLKEDNDKTTY 548
Query: 506 GAFVDVDPRHEKISLRTLVDHSIVESFGGKGKTCITSRVYPTLAVDNDARLYAFNNGTQT 524
GAFVD++P H+ +SLR L+DHS+VESFGGKG+ CITSRVYP LA+ + L+AFN G Q+
Sbjct: 549 GAFVDINP-HQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQS 583
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q67XZ3 | 4.1e-114 | 40.85 | Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3... | [more] |
Q43866 | 1.3e-107 | 42.09 | Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3... | [more] |
Q70XE6 | 1.2e-105 | 39.93 | Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1 | [more] |
Q43089 | 2.1e-102 | 40.15 | Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 P... | [more] |
Q0J360 | 2.0e-100 | 39.31 | Beta-fructofuranosidase, insoluble isoenzyme 7 OS=Oryza sativa subsp. japonica O... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BB78 | 4.6e-193 | 64.88 | LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWINV3-like OS... | [more] |
A0A6J1HFM6 | 1.1e-189 | 63.75 | beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 OS=Cucurbita... | [more] |
A0A2I4JRG1 | 1.4e-189 | 63.78 | Cell wall invertase 4 OS=Cucumis sativus OX=3659 PE=2 SV=1 | [more] |
A0A0A0LQX1 | 1.4e-189 | 63.78 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351670 PE=3 SV=1 | [more] |
A0A6J1HDH1 | 6.9e-189 | 63.41 | beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X1 OS=Cucurbita... | [more] |
Match Name | E-value | Identity | Description | |
XP_038884233.1 | 9.8e-198 | 68.69 | beta-fructofuranosidase, insoluble isoenzyme CWINV3-like [Benincasa hispida] | [more] |
XP_008444460.1 | 9.5e-193 | 64.88 | PREDICTED: LOW QUALITY PROTEIN: beta-fructofuranosidase, insoluble isoenzyme CWI... | [more] |
XP_011649529.2 | 4.4e-190 | 64.12 | beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X2 [Cucumis sativus] | [more] |
XP_022962009.1 | 2.2e-189 | 63.75 | beta-fructofuranosidase, insoluble isoenzyme CWINV3-like isoform X2 [Cucurbita m... | [more] |
XP_004142928.3 | 2.9e-189 | 63.78 | beta-fructofuranosidase, insoluble isoenzyme CWINV3 isoform X1 [Cucumis sativus]... | [more] |